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	<id>http://www.waiwiki.org/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Aytundra</id>
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	<updated>2026-07-27T19:52:52Z</updated>
	<subtitle>User contributions</subtitle>
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	<entry>
		<id>http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5729</id>
		<title>DomesBricksandMore</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5729"/>
		<updated>2016-10-07T21:08:04Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Universal Design Topic&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;In case a pyramid does not work.&lt;br /&gt;
&lt;br /&gt;
Or a structure needs to last a little longer.&lt;br /&gt;
&lt;br /&gt;
Or a hill needs to be built.&lt;br /&gt;
&lt;br /&gt;
How bout a dome?&lt;br /&gt;
-------------&lt;br /&gt;
&lt;br /&gt;
Here are some websites to look at:&lt;br /&gt;
&lt;br /&gt;
http://isha.sadhguru.org/blog/inside-isha/isha-yoga-center/a-labor-of-love-the-dhyanalinga-dome/&lt;br /&gt;
&lt;br /&gt;
Talks about why not to use cement.&lt;br /&gt;
&lt;br /&gt;
Talks about how to make bricks.&lt;br /&gt;
&lt;br /&gt;
The trick to solid bricks is testing it under water for 24 hours, melted bricks are not durable.&lt;br /&gt;
&lt;br /&gt;
Talks about how to make a dome.&lt;br /&gt;
&lt;br /&gt;
The trick to building one layer is to have a layer built all at once.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
(click transcript) http://www.pbs.org/wgbh/nova/ancient/great-cathedral-mystery.html&lt;br /&gt;
&lt;br /&gt;
The trick to positioning the bricks in a dome, is to have ropes from a center on the floor.&lt;br /&gt;
&lt;br /&gt;
The trick to make the bricks slope is a herring bone pattern of layering the bricks. The video actually showed a sample of how reseachers think they builted it on TV.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Tiling&lt;br /&gt;
&lt;br /&gt;
To position tiles in absolute positions; points with Euclidean drawings might help with localizing the points.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SAFETY &amp;amp; ACCESSIBILITY&lt;br /&gt;
&lt;br /&gt;
I have heard/read that design is especially important to the function and safety.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Stairs Safety - Stair height and width:&lt;br /&gt;
&lt;br /&gt;
To prevent tripping and falling, it has been suggested that the steps of a flight of stairs have enough tread (some people have longer feet)&lt;br /&gt;
&lt;br /&gt;
Tread (nosing + run) 279mm, Rise (nosing + riser) 178mm.&lt;br /&gt;
&lt;br /&gt;
(See figure 2) http://www.cmhc-schl.gc.ca/en/co/acho/acho_012.cfm&lt;br /&gt;
&lt;br /&gt;
[Of course it depends on the dimensions and physical abilities of the users; these measurements may have to change to match the range of users.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Stairs Safety - Stair hand rails:&lt;br /&gt;
&lt;br /&gt;
Handrail height 900- 965 mm.&lt;br /&gt;
&lt;br /&gt;
Handrail extension at bottom and top of the stairs.&lt;br /&gt;
&lt;br /&gt;
(See figure 1) http://www.cmhc-schl.gc.ca/en/co/acho/acho_012.cfm&lt;br /&gt;
&lt;br /&gt;
[Of course it depends on the dimensions and physical abilities of the users; these measurements may have to change to match the range of users.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Door Accessibility - Lever is better than Circular knob &lt;br /&gt;
&lt;br /&gt;
For people who have their hands full or injured or with arthritis etc..., lever handles are good for doors.&lt;br /&gt;
&lt;br /&gt;
http://www.dailymail.co.uk/news/article-2512630/Doorknobs-BANNED-Vancouver-local-government-pushes-make-houses-elderly-friendly.html&lt;br /&gt;
&lt;br /&gt;
hth&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
UNIVERSAL DESIGN CONCEPT (a concept from engineering) / Barrier Free Concept(バリアフリ)&lt;br /&gt;
- Universal design is about making the physical environment accessible to the greatest number of people.&lt;br /&gt;
&lt;br /&gt;
i.e. making ramps means that people with wheelchairs, strollers, bikes, skateboards can all have access.&lt;br /&gt;
making a building that only contains stairs will prevent access to the building.&lt;br /&gt;
&lt;br /&gt;
https://en.wikipedia.org/wiki/Universal_design&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=TransferFish&amp;diff=5672</id>
		<title>TransferFish</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=TransferFish&amp;diff=5672"/>
		<updated>2016-09-23T17:22:48Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: How to Transfer fish with minimal stress on the fish&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Regular pet stores and fish aquariums, use a net to transfer fish.&lt;br /&gt;
They dip a mesh net in the tank, corner the fish to a wall of a tank.&lt;br /&gt;
When the fish is inside, the mesh net is hoisted above the water into the air, and the fish will flap its fins, gills and tails.&lt;br /&gt;
That can be traumatic for the fish, not to mention the possibility of losing a fin or gill to the mesh of the net.&lt;br /&gt;
When the fish is returned to water in a different tank, you can see that the fish is probably recovering from the aftermath of such an ordeal.&lt;br /&gt;
The fish may behave in a jumpy startled manner if the water is disturbed in the minutes after a transfer. &lt;br /&gt;
The fish may also detect light and movement outside of the glass of the tank.&lt;br /&gt;
(It is recommended that blue clothing be worn in the presence of fish.&lt;br /&gt;
Avoid spotted pattern clothing.&lt;br /&gt;
Avoid line pattern clothing.&lt;br /&gt;
These patterns may cause fish to react in fear. In general.&lt;br /&gt;
Do research natural predators of these fish, and not wear the colours or patterns of these fish.)&lt;br /&gt;
The fish may often hide behind water filters after a transfer.&lt;br /&gt;
It may take 10 to 30 minutes, before you see the fish behaving more normally.&lt;br /&gt;
&lt;br /&gt;
There is a way to make transfers between waters less stressful.&lt;br /&gt;
When scooping fish with a net, make sure you do not damage their fins.&lt;br /&gt;
Their fins and gills can easily get caught in the net.&lt;br /&gt;
If possible transfer the net with a smaller box under the net, the box holding water from the home tank&lt;br /&gt;
and when you move the net with the box cupping the net, the fish is still in water.&lt;br /&gt;
Then in the new tank the fish can be placed with the box in the water.&lt;br /&gt;
tip the box and the net to release the fish.&lt;br /&gt;
[Imagine an octopus deciding to help humans travel across islands.&lt;br /&gt;
Humans being transferred by a net held from an octopus moving the human from land to ocean to a new land.&lt;br /&gt;
It would probably be harsh if humans have to get wet and feel like drowning each time humans are moved to another land. &lt;br /&gt;
If the octopus transferred the humans from grabbing a glass box, or with a submarine or a plane holding the air of the old land, then it is probably more of a humane way for transfer. &lt;br /&gt;
The purpose of the net is to reduce the space or the spread, just like walkway tunnels can transfer a hall of people via a bridge to an airplane reduces the spread of a crowd to a smaller space. Meanwhile the enclosure a plane or a box around it maintains the fluid that is compatible with the breathing abilities of the living thing transferred.]&lt;br /&gt;
&lt;br /&gt;
Tanks/Ponds/Environment&lt;br /&gt;
Make sure the temperature is similar.&lt;br /&gt;
Make sure the new tank or environment is better than the previous environment.&lt;br /&gt;
Allow for the water chemistry to be similar, so that the fish can acclimatize more easily.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5404</id>
		<title>DomesBricksandMore</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5404"/>
		<updated>2016-01-12T16:16:32Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Safety Accessibility, stairs, doors.&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;In case a pyramid does not work.&lt;br /&gt;
&lt;br /&gt;
Or a structure needs to last a little longer.&lt;br /&gt;
&lt;br /&gt;
Or a hill needs to be built.&lt;br /&gt;
&lt;br /&gt;
How bout a dome?&lt;br /&gt;
-------------&lt;br /&gt;
&lt;br /&gt;
Here are some websites to look at:&lt;br /&gt;
&lt;br /&gt;
http://isha.sadhguru.org/blog/inside-isha/isha-yoga-center/a-labor-of-love-the-dhyanalinga-dome/&lt;br /&gt;
&lt;br /&gt;
Talks about why not to use cement.&lt;br /&gt;
&lt;br /&gt;
Talks about how to make bricks.&lt;br /&gt;
&lt;br /&gt;
The trick to solid bricks is testing it under water for 24 hours, melted bricks are not durable.&lt;br /&gt;
&lt;br /&gt;
Talks about how to make a dome.&lt;br /&gt;
&lt;br /&gt;
The trick to building one layer is to have a layer built all at once.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
(click transcript) http://www.pbs.org/wgbh/nova/ancient/great-cathedral-mystery.html&lt;br /&gt;
&lt;br /&gt;
The trick to positioning the bricks in a dome, is to have ropes from a center on the floor.&lt;br /&gt;
&lt;br /&gt;
The trick to make the bricks slope is a herring bone pattern of layering the bricks. The video actually showed a sample of how reseachers think they builted it on TV.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Tiling&lt;br /&gt;
&lt;br /&gt;
To position tiles in absolute positions; points with Euclidean drawings might help with localizing the points.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SAFETY &amp;amp; ACCESSIBILITY&lt;br /&gt;
&lt;br /&gt;
I have heard/read that design is especially important to the function and safety.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Stairs Safety - Stair height and width:&lt;br /&gt;
&lt;br /&gt;
To prevent tripping and falling, it has been suggested that the steps of a flight of stairs have enough tread (some people have longer feet)&lt;br /&gt;
&lt;br /&gt;
Tread (nosing + run) 279mm, Rise (nosing + riser) 178mm.&lt;br /&gt;
&lt;br /&gt;
(See figure 2) http://www.cmhc-schl.gc.ca/en/co/acho/acho_012.cfm&lt;br /&gt;
&lt;br /&gt;
[Of course it depends on the dimensions and physical abilities of the users; these measurements may have to change to match the range of users.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Stairs Safety - Stair hand rails:&lt;br /&gt;
&lt;br /&gt;
Handrail height 900- 965 mm.&lt;br /&gt;
&lt;br /&gt;
Handrail extension at bottom and top of the stairs.&lt;br /&gt;
&lt;br /&gt;
(See figure 1) http://www.cmhc-schl.gc.ca/en/co/acho/acho_012.cfm&lt;br /&gt;
&lt;br /&gt;
[Of course it depends on the dimensions and physical abilities of the users; these measurements may have to change to match the range of users.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Door Accessibility - Lever is better than Circular knob &lt;br /&gt;
&lt;br /&gt;
For people who have their hands full or injured or with arthritis etc..., lever handles are good for doors.&lt;br /&gt;
&lt;br /&gt;
http://www.dailymail.co.uk/news/article-2512630/Doorknobs-BANNED-Vancouver-local-government-pushes-make-houses-elderly-friendly.html&lt;br /&gt;
&lt;br /&gt;
hth&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5363</id>
		<title>DomesBricksandMore</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5363"/>
		<updated>2016-01-08T21:55:04Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spacing&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;In case a pyramid does not work.&lt;br /&gt;
&lt;br /&gt;
Or a structure needs to last a little longer.&lt;br /&gt;
&lt;br /&gt;
Or a hill needs to be built.&lt;br /&gt;
&lt;br /&gt;
How bout a dome?&lt;br /&gt;
-------------&lt;br /&gt;
&lt;br /&gt;
Here are some websites to look at:&lt;br /&gt;
&lt;br /&gt;
http://isha.sadhguru.org/blog/inside-isha/isha-yoga-center/a-labor-of-love-the-dhyanalinga-dome/&lt;br /&gt;
&lt;br /&gt;
Talks about why not to use cement.&lt;br /&gt;
&lt;br /&gt;
Talks about how to make bricks.&lt;br /&gt;
&lt;br /&gt;
The trick to solid bricks is testing it under water for 24 hours, melted bricks are not durable.&lt;br /&gt;
&lt;br /&gt;
Talks about how to make a dome.&lt;br /&gt;
&lt;br /&gt;
The trick to building one layer is to have a layer built all at once.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
(click transcript) http://www.pbs.org/wgbh/nova/ancient/great-cathedral-mystery.html&lt;br /&gt;
&lt;br /&gt;
The trick to positioning the bricks in a dome, is to have ropes from a center on the floor.&lt;br /&gt;
&lt;br /&gt;
The trick to make the bricks slope is a herring bone pattern of layering the bricks. The video actually showed a sample of how reseachers think they builted it on TV.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Tiling&lt;br /&gt;
&lt;br /&gt;
To position tiles in absolute positions; points with Euclidean drawings might help with localizing the points.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
hth&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5362</id>
		<title>DomesBricksandMore</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=DomesBricksandMore&amp;diff=5362"/>
		<updated>2016-01-08T21:52:18Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Added Links&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;In case a pyramid does not work.&lt;br /&gt;
Or a structure needs to last a little longer.&lt;br /&gt;
Or a hill needs to be built.&lt;br /&gt;
How bout a dome?&lt;br /&gt;
&lt;br /&gt;
Here are some websites to look at:&lt;br /&gt;
http://isha.sadhguru.org/blog/inside-isha/isha-yoga-center/a-labor-of-love-the-dhyanalinga-dome/&lt;br /&gt;
Talks about why not to use cement.&lt;br /&gt;
Talks about how to make bricks.&lt;br /&gt;
The trick to solid bricks is testing it under water for 24 hours, melted bricks are not durable.&lt;br /&gt;
Talks about how to make a dome.&lt;br /&gt;
The trick to building one layer is to have a layer built all at once.&lt;br /&gt;
&lt;br /&gt;
http://www.pbs.org/wgbh/nova/ancient/great-cathedral-mystery.html&lt;br /&gt;
The trick to positioning the bricks in a dome, is to have ropes from a center on the floor.&lt;br /&gt;
The trick to make the bricks slope is a herring bone pattern of layering the bricks. The video actually showed a sample of how reseachers think they builted it on TV.&lt;br /&gt;
&lt;br /&gt;
hth&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5141</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5141"/>
		<updated>2015-11-08T02:59:56Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: +1 article piggy&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;INTRO &lt;br /&gt;
&lt;br /&gt;
The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6 - 30, 2015 | Nov 7 to 30, 2015&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Oxalate topic on WaiWiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture. &lt;br /&gt;
&lt;br /&gt;
Key Question: Does OJ increase kidney stone formation?&lt;br /&gt;
&lt;br /&gt;
Hypothesis 0: OJ does not increase kidney stone.&lt;br /&gt;
&lt;br /&gt;
Hypothesis 1: OJ decrease kidney stone formation.&lt;br /&gt;
&lt;br /&gt;
Hypothesis 2: OJ increase kidney stone formation.&lt;br /&gt;
&lt;br /&gt;
Method: Literature search.&lt;br /&gt;
&lt;br /&gt;
Article_Hypothesis 0: Unknown if science literature provides enough articles to answer the question. {current state of Aytundra Sept 27, 2015, arrgh and I have 3 more days before my self imposed due date... lol. }.&lt;br /&gt;
&lt;br /&gt;
Article_Hypothesis 1: Science literature does provide enough articles to answer the question.&lt;br /&gt;
&lt;br /&gt;
Article_Hypothesis 2: Science literature does not provide enough articles to answer the question.&lt;br /&gt;
&lt;br /&gt;
Results:&lt;br /&gt;
&lt;br /&gt;
Discussion:&lt;br /&gt;
&lt;br /&gt;
Conclusion: Three possible answers, one of them will be choosen:&lt;br /&gt;
&lt;br /&gt;
1. It is okay to drink OJ! {we will find out.}&lt;br /&gt;
&lt;br /&gt;
vs.&lt;br /&gt;
&lt;br /&gt;
2. It is not okay to drink OJ! {we will find out.}&lt;br /&gt;
&lt;br /&gt;
vs.&lt;br /&gt;
&lt;br /&gt;
3. Science literature is currently in crappy state, and we don&amp;#039;t have the answer on my silly question, so keep drinking OJ. {That would be frustrating and a waste of time, to have spent time reading all those articles.}&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
Legend:&lt;br /&gt;
&lt;br /&gt;
(~) = articles are free&lt;br /&gt;
&lt;br /&gt;
[~] = articles are locked (database access required, citation will be from abstract (as abstracts are public) on Waiwiki.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from RRM on Aytundra&amp;#039;s Diary in August 2015:&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
[~] http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from Aytundra after more database searching September 13, 2015:&lt;br /&gt;
&lt;br /&gt;
[~] Baxmann, A.C., De O G Mendonca, C.,and Heilberg, I. P.(2003). Effect of vitamin C supplements on urinary oxalate and pH in calcium stone-forming patients. &amp;#039;&amp;#039;Kidney International&amp;#039;&amp;#039;, Vol. 63 pp. 1066-1071. DOI: 10.1046/j.1523-1755.2003.00815.x &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
My numbering system to organize ideas: &amp;quot;#.#.#.#.&amp;quot; = Category of external input (Vit C input and oxalate input)| Subcategories of external input (type of Vitamin C input, and type of Oxalate input)| mechanism (Oxalate stone formed because &amp;quot;...&amp;quot;)| oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C converts to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol converts to oxalate endogenously; Ethylene_glycol poisoning (via antifreeze)as a precursor for alcohol dehydrogenase to convert glycolate into oxalate.(Ethylene_glycol-alcoholdehydrogenase_to_glycolate-sourced-oxalate). {It is interesting to note that alcohol consumption, is correlated with lower rates of kidney stones in vitamin C consuming individuals [citation required].}&lt;br /&gt;
&lt;br /&gt;
5. Vitamin C converts to oxalate exogenously (in vitro); Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
1.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
1.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
1.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
3.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
3.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.0.0.1. Excretion of urine oxalate is plant-based-sourced-oxalate?&lt;br /&gt;
&lt;br /&gt;
1.0.0.2. Excretion of urine oxalate is C13-sourced-oxalate?&lt;br /&gt;
&lt;br /&gt;
2.0.0.1.&lt;br /&gt;
&lt;br /&gt;
3.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate. &amp;lt;--- {That which oj drinkers might be scared of. But is it true? We will find out after analyzing articles.}&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
5.0.1.5. HCl added to urine sample prevents oxalate formation in stored urine sample.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro [in glass] resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 day interval&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported;then we cannot rule out 1.0.0.0.&amp;#039;s oxalate_as_source. But it is pointless, because this is an &amp;quot;in vitro&amp;quot; experiment.&lt;br /&gt;
&lt;br /&gt;
***** {In vitro means in glass, that means that these urine samples were tampered(experimented) with Vitamin C after sample collection.&lt;br /&gt;
We know from later studies (i.e. see 2003 below) that oxalate can form in the urine sample exogenously. That means we might have to be careful in reading literature.}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
&amp;quot;...Vitamin C supplementation may increase urinary oxalate excretion and the risk of calcium oxalate crystallization in calcium stone-forming patients.&amp;quot;(Baxmann, A.C., et al 2003, p.1066 abstract).&lt;br /&gt;
&lt;br /&gt;
47 stone forming patients&lt;br /&gt;
&lt;br /&gt;
Vitamin C supplement&lt;br /&gt;
&lt;br /&gt;
2003&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
***** Study controlled for oxalate levels in vitro after sampling. They had control subjects provide additional samples of urine; in which they added HCl to urine sampling cup right after collection. {This shows that in 2003, at least some literature on the topic of vitamin C and oxalate formation, are aware of, and controls for defining if oxalate is produced in vivo or in vitro.}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
--------------&lt;br /&gt;
&lt;br /&gt;
Other Animals: Vitamin C &amp;amp; Oxalate levels in cats, pigs...&lt;br /&gt;
&lt;br /&gt;
-----&lt;br /&gt;
&lt;br /&gt;
PMID: 15309947 &lt;br /&gt;
Pion, S.J. (2004) Effects of vitamin C supplementation on plasma ascorbic acid and oxalate concentrations and meat quality in swine. &amp;#039;&amp;#039;Journal of Animal Science&amp;#039;&amp;#039; 82(7).&lt;br /&gt;
{In Pion, S.J.&amp;#039;s 2014 article they found: Vitamin C supplementation elevated cortisol levels in pigs 96 hrs post 48 hrs supplementation.&lt;br /&gt;
Oxalate levels were not elevated in pigs supplemented with vitamin C. Researchers were hoping to obtain oxalate levels in the meat to have better meat quality.}&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Composting_toilet_scale_model&amp;diff=5075</id>
		<title>Composting toilet scale model</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Composting_toilet_scale_model&amp;diff=5075"/>
		<updated>2015-09-28T15:00:54Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;This page is an extension of the page on [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets].&lt;br /&gt;
&lt;br /&gt;
[[Image:001.jpg|thumb|left| miniature toilet; the big hole is for fecal matter, and the small hole for urine diverted by the yellow tube ]]&lt;br /&gt;
[[Image:003.jpg|thumb|right| &amp;#039;The Slide&amp;#039;; concrete base with 2 wooden shutters ]]&lt;br /&gt;
[[Image:002.jpg|thumb|left| ]]&lt;br /&gt;
[[Image:004.jpg|thumb|right| toilet in concrete floor placed on top of concrete slide ]]&lt;br /&gt;
[[Image:005.jpg|thumb|left| toilet in concrete floor placed on top of concrete slide ]]&lt;br /&gt;
&lt;br /&gt;
==Parts== &lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
* The big hole (fecal hole) is for collecting faeces, which goes straight down to the floor of the slide&lt;br /&gt;
* The small hole (urine hole) in the toilet is for collecting and diverting urine&lt;br /&gt;
* The yellow tube is the tube that drains the urine from the toilet to the urine collection barrel&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
==Additional Parts==&lt;br /&gt;
&lt;br /&gt;
* The urine collection barrel is placed at the downside of the slide.  &lt;br /&gt;
* The black pipes are for solar ventilation. They are metal / aluminium pipes that penetrate the cover of the slide at its downside, to suck up the relatively cold air that came in through the fecal toilet opening. The sucking draft is generated by the upwards movement of the air through the black pipes, caused by the heating of the pipes and the air inside through sunlight exposure. Relatively hot air goes up. Relatively cold air goes down. T-junctions are attached on the top-end of the ventilation pipes to let the air go out while preventing raindrops from coming in. The ventilation pipes are painted black to maximize heating by the sun. The total surface volume of the ventilation pipes (probably 3 or 4 pipes) needs to match the surface volume of the fecal hole to create optimal air draft.&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
==The Numbered Parts==&lt;br /&gt;
[[Image:007b.jpg|thumb|right| T-splitters on top of ventilation pipes ]]&lt;br /&gt;
[[Image:007.jpg|thumb|left| sideview ]]&lt;br /&gt;
[[Image:006.jpg|thumb|right| view from the front ]]&lt;br /&gt;
[[Image:009.jpg|thumb|left| bottom, back-side view ]]&lt;br /&gt;
[[Image:008.jpg|thumb|right| sideview ]]&lt;br /&gt;
[[Image:012.jpg|thumb|left| back-, sideview ]]&lt;br /&gt;
[[Image:010.jpg|thumb|right| sideview ]]&lt;br /&gt;
[[Image:014.jpg|thumb|left| view from the top, front-side ]]&lt;br /&gt;
[[Image:013.jpg|thumb|right| view from the top ]]&lt;br /&gt;
[[Image:016.jpg|thumb|left| view from the back ]]&lt;br /&gt;
[[Image:015.jpg|thumb|right| view from the back ]]&lt;br /&gt;
&lt;br /&gt;
* 1 is the concrete bottom of the slide, measured as in between side 2 and side 3, which is 100 centimeter wide (in between walls). It is about 260 centimeter long. In reality, the sides 2 and 3 will be constructed on top of the concrete base (part 1), and for stability of the entire construction, the actual width of this base may be much more than 120 cm.&lt;br /&gt;
* 2 and 3 are the concrete sides of the slide. Where connected to part 1, they are about 260 cm long. They may be 80 to 120 cm high.&lt;br /&gt;
* 4 is the back side of the concrete slide, on the upper side of the slide. It is about 63 cm high, and about 120 cm wide (100 + 10 + 10), depending on the thickness of the concrete blocks used for part 2 and 3. In reality, part 4 will be constructed on top of the concrete base (part 1), and the actual height of part 4 will therefore be closer to 50 cm.&lt;br /&gt;
* 5 is the upper part of the cover on the slide; either concrete or wood. It is about 120 cm wide (100 + 10 + 10), depending on the thickness of the concrete blocks used for part 2 and 3.&lt;br /&gt;
* 6 is the (wooden or concrete) part next to the inspection shutter that partly covers the slide. The ventilation pipes will run across 6.&lt;br /&gt;
* 7 is the inspection shutter that partly covers the slide. Isolation material needs to keep the slide air-tight (apart from the fecal hole and the ventilation pipes), to prevent unwanted air-draft&lt;br /&gt;
* 8 is the lower part of the cover on the slide; either concrete or wood&lt;br /&gt;
* 9 is the shutter at the bottom of the slide; for removal of the compost (end product). Isolation material needs to keep the slide air-tight (apart from the fecal hole and the ventilation pipes), to prevent unwanted air-draft&lt;br /&gt;
* 10 is the concrete floor of the lavatory cabin&lt;br /&gt;
* 11, 12, 13 and 14 are the walls of the lavatory cabin (13 and 14 do not exist in the pics)&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5073</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5073"/>
		<updated>2015-09-27T23:51:52Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling, Format, and  More thoughts&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;INTRO &lt;br /&gt;
&lt;br /&gt;
The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Oxalate topic on WaiWiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture. &lt;br /&gt;
&lt;br /&gt;
Key Question: Does OJ increase kidney stone formation?&lt;br /&gt;
&lt;br /&gt;
Hypothesis 0: OJ does not increase kidney stone.&lt;br /&gt;
&lt;br /&gt;
Hypothesis 1: OJ decrease kidney stone formation.&lt;br /&gt;
&lt;br /&gt;
Hypothesis 2: OJ increase kidney stone formation.&lt;br /&gt;
&lt;br /&gt;
Method: Literature search.&lt;br /&gt;
&lt;br /&gt;
Article_Hypothesis 0: Unknown if science literature provides enough articles to answer the question. {current state of Aytundra Sept 27, 2015, arrgh and I have 3 more days before my self imposed due date... lol. }.&lt;br /&gt;
&lt;br /&gt;
Article_Hypothesis 1: Science literature does provide enough articles to answer the question.&lt;br /&gt;
&lt;br /&gt;
Article_Hypothesis 2: Science literature does not provide enough articles to answer the question.&lt;br /&gt;
&lt;br /&gt;
Results:&lt;br /&gt;
&lt;br /&gt;
Discussion:&lt;br /&gt;
&lt;br /&gt;
Conclusion: Three possible answers, one of them will be choosen:&lt;br /&gt;
&lt;br /&gt;
1. It is okay to drink OJ! {we will find out.}&lt;br /&gt;
&lt;br /&gt;
vs.&lt;br /&gt;
&lt;br /&gt;
2. It is not okay to drink OJ! {we will find out.}&lt;br /&gt;
&lt;br /&gt;
vs.&lt;br /&gt;
&lt;br /&gt;
3. Science literature is currently in crappy state, and we don&amp;#039;t have the answer on my silly question, so keep drinking OJ. {That would be frustrating and a waste of time, to have spent time reading all those articles.}&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
Legend:&lt;br /&gt;
&lt;br /&gt;
(~) = articles are free&lt;br /&gt;
&lt;br /&gt;
[~] = articles are locked (database access required, citation will be from abstract (as abstracts are public) on Waiwiki.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from RRM on Aytundra&amp;#039;s Diary in August 2015:&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
[~] http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from Aytundra after more database searching September 13, 2015:&lt;br /&gt;
&lt;br /&gt;
[~] Baxmann, A.C., De O G Mendonca, C.,and Heilberg, I. P.(2003). Effect of vitamin C supplements on urinary oxalate and pH in calcium stone-forming patients. &amp;#039;&amp;#039;Kidney International&amp;#039;&amp;#039;, Vol. 63 pp. 1066-1071. DOI: 10.1046/j.1523-1755.2003.00815.x &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
My numbering system to organize ideas: &amp;quot;#.#.#.#.&amp;quot; = Category of external input (Vit C input and oxalate input)| Subcategories of external input (type of Vitamin C input, and type of Oxalate input)| mechanism (Oxalate stone formed because &amp;quot;...&amp;quot;)| oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C converts to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol converts to oxalate endogenously; Ethylene_glycol poisoning (via antifreeze)as a precursor for alcohol dehydrogenase to convert glycolate into oxalate.(Ethylene_glycol-alcoholdehydrogenase_to_glycolate-sourced-oxalate). {It is interesting to note that alcohol consumption, is correlated with lower rates of kidney stones in vitamin C consuming individuals [citation required].}&lt;br /&gt;
&lt;br /&gt;
5. Vitamin C converts to oxalate exogenously (in vitro); Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
1.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
1.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
1.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
3.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
3.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.0.0.1. Excretion of urine oxalate is plant-based-sourced-oxalate?&lt;br /&gt;
&lt;br /&gt;
1.0.0.2. Excretion of urine oxalate is C13-sourced-oxalate?&lt;br /&gt;
&lt;br /&gt;
2.0.0.1.&lt;br /&gt;
&lt;br /&gt;
3.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate. &amp;lt;--- {That which oj drinkers might be scared of. But is it true? We will find out after analyzing articles.}&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
5.0.1.5. HCl added to urine sample prevents oxalate formation in stored urine sample.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro [in glass] resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 day interval&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported;then we cannot rule out 1.0.0.0.&amp;#039;s oxalate_as_source. But it is pointless, because this is an &amp;quot;in vitro&amp;quot; experiment.&lt;br /&gt;
&lt;br /&gt;
***** {In vitro means in glass, that means that these urine samples were tampered(experimented) with Vitamin C after sample collection.&lt;br /&gt;
We know from later studies (i.e. see 2003 below) that oxalate can form in the urine sample exogenously. That means we might have to be careful in reading literature.}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
&amp;quot;...Vitamin C supplementation may increase urinary oxalate excretion and the risk of calcium oxalate crystallization in calcium stone-forming patients.&amp;quot;(Baxmann, A.C., et al 2003, p.1066 abstract).&lt;br /&gt;
&lt;br /&gt;
47 stone forming patients&lt;br /&gt;
&lt;br /&gt;
Vitamin C supplement&lt;br /&gt;
&lt;br /&gt;
2003&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
***** Study controlled for oxalate levels in vitro after sampling. They had control subjects provide additional samples of urine; in which they added HCl to urine sampling cup right after collection. {This shows that in 2003, at least some literature on the topic of vitamin C and oxalate formation, are aware of, and controls for defining if oxalate is produced in vivo or in vitro.}&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5027</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5027"/>
		<updated>2015-09-15T19:00:12Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: editing&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;INTRO &lt;br /&gt;
&lt;br /&gt;
The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
Legend:&lt;br /&gt;
&lt;br /&gt;
(~) = articles are free&lt;br /&gt;
&lt;br /&gt;
[~] = articles are locked (database access required, citation will be from abstract (as abstracts are public) on Waiwiki.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from RRM on Aytundra&amp;#039;s Diary in August 2015:&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from Aytundra after more database searching September 13, 2015:&lt;br /&gt;
&lt;br /&gt;
[~] Baxmann, A.C., De O G Mendonca, C.,and Heilberg, I. P.(2003). Effect of vitamin C supplements on urinary oxalate and pH in calcium stone-forming patients. &amp;#039;&amp;#039;Kidney International&amp;#039;&amp;#039;, Vol. 63 pp. 1066-1071. DOI: 10.1046/j.1523-1755.2003.00815.x &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
My numbering system to organize ideas: &amp;quot;#.#.#.#.&amp;quot; = Category of external input| Subcategories of external input| mechanism internal | oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C converts to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol converts to oxalate endogenously; Ethylene_glycol poisoning (via antifreeze)as a precursor for alcohol dehydrogenase to convert glycolate into oxalate.(Ethylene_glycol-alcoholdehydrogenase_to_glycolate-sourced-oxalate). {It is interesting to note that alcohol consumption, is correlated with lower rats of kidney stones in vitamin C consuming individuals [citation required].}&lt;br /&gt;
&lt;br /&gt;
5. Vitamin C converts to oxalate exogenously (in vitro); Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
1.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
1.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
1.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
3.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
3.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.0.0.2. Excretion of urine oxalate is plant-based-sourced-oxalate.&lt;br /&gt;
&lt;br /&gt;
3.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate. &amp;lt;--- {That which oj drinkers might be scared of. But is it true? We will find out after analyzing articles.}&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
5.0.1.0. HCl added to urine sample prevents oxalate formation in stored urine sample.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
&amp;quot;...Vitamin C supplementation may increase urinary oxalate excretion and the risk of calcium oxalate crystallization in calcium stone-forming patients.&amp;quot;(Baxmann, A.C., et al 2003, p.1066 abstract).&lt;br /&gt;
&lt;br /&gt;
47 stone forming patients&lt;br /&gt;
&lt;br /&gt;
Vitamin C supplement&lt;br /&gt;
&lt;br /&gt;
2003&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
***** Study controlled for oxalate levels in vitro after sampling. They had control subjects provide additional samples of urine; in which they added HCl to urine sampling cup right after collection. {This shows that in 2003, at least some literature on the topic of vitamin C and oxalate formation, are aware of, and controls for defining if oxalate is produced in vivo or in vitro.}&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5022</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5022"/>
		<updated>2015-09-13T22:57:40Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: positioning&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Going to test Wiki&amp;#039;s ability to organize sources.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
TO BEGIN&lt;br /&gt;
&lt;br /&gt;
DUMPING ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
Legend:&lt;br /&gt;
&lt;br /&gt;
(~) = articles are free&lt;br /&gt;
&lt;br /&gt;
[~] = articles are locked (database access required, citation will be from abstract (as abstracts are public) only on this Waiwiki.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from RRM on Aytundra&amp;#039;s Diary in August 2015:&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from Aytundra after more database searching September 13, 2015:&lt;br /&gt;
&lt;br /&gt;
[~] Baxmann, A.C., De O G Mendonca, C.,and Heilberg, I. P.(2003). Effect of vitamin C supplements on urinary oxalate and pH in calcium stone-forming patients. &amp;#039;&amp;#039;Kidney International&amp;#039;&amp;#039;, Vol. 63 pp. 1066-1071. DOI: 10.1046/j.1523-1755.2003.00815.x &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
My numbering system to organize ideas: &amp;quot;#.#.#.#.&amp;quot; = Category of external input| Subcategories of external input| mechanism internal | oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C converts to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol converts to oxalate endogenously; Ethylene_glycol poisoning (via antifreeze)as a precursor for alcohol dehydrogenase to convert glycolate into oxalate.(Ethylene_glycol-alcoholdehydrogenase_to_glycolate-sourced-oxalate). {It is interesting to note that alcohol consumption, is correlated with lower kidney stones in vitamin C individuals [citation required].}&lt;br /&gt;
&lt;br /&gt;
5. Vitamin C converts to oxalate exogenously (in vitro); Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
1.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
1.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
1.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
3.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
3.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.0.0.2. Excretion of urine oxalate is plant-based-sourced-oxalate.&lt;br /&gt;
&lt;br /&gt;
3.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate. &amp;lt;--- {That which oj drinkers might be scared of. But is it true? We will find out after analyzing articles.}&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
&amp;quot;...Vitamin C supplementation may increase urinary oxalate excretion and the risk of calcium oxalate crystallization in calcium stone-forming patients.&amp;quot;(Baxmann, A.C., et al 2003, p.1066 abstract).&lt;br /&gt;
&lt;br /&gt;
47 stone forming patients&lt;br /&gt;
&lt;br /&gt;
Vitamin C supplement&lt;br /&gt;
&lt;br /&gt;
2003&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
***** Study controlled for oxalate levels in vitro after sampling. They had control subjects provide additional samples of urine; in which they added HCl to urine sampling cup right after collection. {This shows that in 2003, at least some literature on the topic of vitamin C and oxalate formation, are aware of, and controls for defining if oxalate is produced in vivo or in vitro.}&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5021</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5021"/>
		<updated>2015-09-13T22:54:50Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Reorganize some sentence positioning. Added 1 article.&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Going to test Wiki&amp;#039;s ability to organize sources.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
TO BEGIN&lt;br /&gt;
&lt;br /&gt;
DUMPING ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
Legend:&lt;br /&gt;
(~) = articles are free&lt;br /&gt;
[~] = articles are locked (database access required, citation will be from abstract only on this Waiwiki.)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from RRM on Aytundra&amp;#039;s Diary in August 2015:&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Links from Aytundra after more database searching September 13, 2015:&lt;br /&gt;
&lt;br /&gt;
[~] Baxmann, A.C., De O G Mendonca, C.,and Heilberg, I. P.(2003). Effect of vitamin C supplements on urinary oxalate and pH in calcium stone-forming patients. &amp;#039;&amp;#039;Kidney International&amp;#039;&amp;#039;, Vol. 63 pp. 1066-1071. DOI: 10.1046/j.1523-1755.2003.00815.x &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
My numbering system to organize ideas: &amp;quot;#.#.#.#.&amp;quot; = Category of external input| Subcategories of external input| mechanism internal | oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C converts to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol converts to oxalate endogenously; Ethylene_glycol poisoning (via antifreeze)as a precursor for alcohol dehydrogenase to convert glycolate into oxalate.(Ethylene_glycol-alcoholdehydrogenase_to_glycolate-sourced-oxalate). {It is interesting to note that alcohol consumption, is correlated with lower kidney stones in vitamin C individuals [citation required].}&lt;br /&gt;
&lt;br /&gt;
5. Vitamin C converts to oxalate exogenously (in vitro); Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
1.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
1.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
1.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
3.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
3.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
1.0.0.2. Excretion of urine oxalate is plant-based-sourced-oxalate.&lt;br /&gt;
&lt;br /&gt;
3.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate. &amp;lt;--- {That which oj drinkers might be scared of. But is it true? We will find out after analyzing articles.}&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
1996&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
------&lt;br /&gt;
&lt;br /&gt;
&amp;quot;...Vitamin C supplementation may increase urinary oxalate excretion and the risk of calcium oxalate crystallization in calcium stone-forming patients.&amp;quot;(Baxmann, A.C., et al 2003, p.1066 abstract).&lt;br /&gt;
&lt;br /&gt;
47 stone forming patients&lt;br /&gt;
&lt;br /&gt;
Vitamin C supplement&lt;br /&gt;
&lt;br /&gt;
2003&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
***** Study controlled for oxalate levels in vitro after sampling. They had control subjects provide additional samples of urine; in which they added HCl to urine sampling cup right after collection. {This shows that in 2003, at least some literature on the topic of vitamin C and oxalate formation, are aware of, and controls for defining if oxalate is produced in vivo or in vitro.}&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5020</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5020"/>
		<updated>2015-09-06T19:38:57Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spacing&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Going to test Wiki&amp;#039;s ability to organize sources.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
TO BEGIN&lt;br /&gt;
&lt;br /&gt;
DUMPING ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
My numbering system to organize ideas: &amp;quot;#.#.#.#.&amp;quot; = Category of oxalate from urine sample external | Vitamin C, Oxalate subcategories input| mechanism internal|oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C converted to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol poisoning as precursor for alcohol dehydrogenase to convert glycolate into oxalate.(ethylene_glycol-glycolate-oxalate) antifreeze (Ethylene_glycol-alcoholdehydrogenase-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
5. Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
1.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
1.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
2.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
2.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
2.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
2.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
1.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate. &amp;lt;--- {That which oj drinkers might be scared of. But is it true? We will find out after analyzing articles.}&lt;br /&gt;
&lt;br /&gt;
1.0.0.2. Excretion of urine oxalate is plant-based-sourced-oxalate.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5019</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5019"/>
		<updated>2015-09-06T19:28:13Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Classified sources of Oxalate. Scrutinized articles. Questions for articles.&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Going to test Wiki&amp;#039;s ability to organize sources.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - Aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
TO BEGIN&lt;br /&gt;
&lt;br /&gt;
DUMPING ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
OTHER THINGS TO READ:&lt;br /&gt;
&lt;br /&gt;
This link gives exact oxalate/100 g for legumes, nuts and flours.&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
This link gives approximate oxalate/100 g for various foods.&lt;br /&gt;
&lt;br /&gt;
http://www.denvernephrology.com/wp-content/uploads/2012/12/Oxalate2008.pdf&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
CATEGORIES OF OXALATES defined by Aytundra.&lt;br /&gt;
&lt;br /&gt;
Oxalate could be in a urine sample because:&lt;br /&gt;
&lt;br /&gt;
1. Vitamin C converted to oxalate endogenously (Vitamin_C-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
2. Vitamin C mobilizes oxalate from foods in the diet (plant_based_veggie-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
3. Vitamin C mobilizes oxalate from oxalate supplements in the diet (C13_marker-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
4. Ethylene_glycol poisoning as precursor for alcohol dehydrogenase to convert glycolate into oxalate.(ethylene_glycol-glycolate-oxalate) antifreeze (Ethylene_glycol-alcoholdehydrogenase-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
5. Error in Urine Collection Procedure (Vitamin_C-procedure-sourced-oxalate).&lt;br /&gt;
&lt;br /&gt;
#.#.#.#. = Category of oxalate from urine sample external | Vitamin C, Oxalate subcategories input| mechanism internal|oxalate subcategories output &lt;br /&gt;
&lt;br /&gt;
1.1 (orange_juice-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
1.2 (supplement-based-Vitamin_C)&lt;br /&gt;
&lt;br /&gt;
2.1 (seed-plant-based-oxalate) pepper.&lt;br /&gt;
&lt;br /&gt;
2.2 (peel-plant-based-oxalate) lemon peel.&lt;br /&gt;
&lt;br /&gt;
2.3 (leaf-plant-based-oxalate) amaranth leaf.&lt;br /&gt;
&lt;br /&gt;
2.4 (stem-plant-based-oxalate).&lt;br /&gt;
&lt;br /&gt;
4.0.1.0. Ethylene glycol&amp;#039;s oxalic acid is produced from alcohol dehydrogenase.&lt;br /&gt;
&lt;br /&gt;
1.0.0.1. Excretion of urine oxalate is Vitamin-C-sourced-oxalate.&lt;br /&gt;
&lt;br /&gt;
1.0.0.1. Excretion of urine oxalate is plant-based-sourced-oxalate.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
SCRUTINIZING ARTICLES&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
Ethylene glycol combined with alcohol dehydrogenase metabolizes to glucolaldehyde to glycolate and including one to oxalate(Jacobsen D. and McMartin K.E., 1986). http://www.ncbi.nlm.nih.gov/pubmed/3537623&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** Aytundra is thinking: {Would drinking alcohol or fermented orange juice help reduce vitamin C conversion to oxalic acid? Is that why vitamin C supplements &lt;br /&gt;
bring out urinary oxalate?}&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
&amp;quot; Ascorbate added directly to urine in vitro resulted in statistically significant but modest increases in measured oxalate. Addition of 5.68 mmol./l. ascorbate increased measured urinary oxalate by 36 mumol./l., implying conversion of ascorbate to oxalate during analysis. Measurement of 24-hour urinary oxalate levels with 5 and 10 gm. ascorbate per day showed similar, modest increases,...&amp;quot; (Wandzilak T.R., D&amp;#039;Andre S.D., Davis P.A., Williams H.E., 1994).&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
1 gm&lt;br /&gt;
&lt;br /&gt;
5 gm&lt;br /&gt;
&lt;br /&gt;
10 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
1994&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
-------&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
&amp;quot;At the vitmamin C dose of 1000 mg daily, urine uric acid and oxalate were elevated.&amp;quot;(Levine M. et al, 1996)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/?page=5&lt;br /&gt;
&lt;br /&gt;
7 male adults without kidney stone history.&lt;br /&gt;
&lt;br /&gt;
1996, study was published.&lt;br /&gt;
&lt;br /&gt;
300 food items&lt;br /&gt;
&lt;br /&gt;
14 day rotation menu&lt;br /&gt;
&lt;br /&gt;
4 to 6 months hospital controlled diet.&lt;br /&gt;
&lt;br /&gt;
***The key questions that Aytundra wants to ask Levine et al. 1996: What foods did each of these 7 male adults eat from the 300 food items offered by the hospital? and of these food items eaten, What was the total oxalate content per day per individual during the course of the study?&amp;quot;&lt;br /&gt;
&lt;br /&gt;
**** Flaw: Diets of subjects were not reported.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The results showed that erroneously high analytical oxalate levels occur in the a[b]sence[sic] of preservative. In the preserved samples there was no significant increase in oxalate excretion at any stage of the protocol. (Auer B.L., Auer D., and Rodgers A.L., 1998). &amp;quot;http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&amp;quot;There were no changes in either the calcium oxalate relative supersaturation or Tiselius risk index. It is concluded that ingestion of large doses of ascorbic acid does not affect the principal risk factors associated with calcium oxalate kidney stone formation.&amp;quot; (Auer B.L., Auer D., and Rodgers A.L., 1998). http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
10 healthy males&lt;br /&gt;
&lt;br /&gt;
4 gm&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
5 days&lt;br /&gt;
&lt;br /&gt;
1998&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
***** However, study points to the protocol of sample preservation as key to understanding oxalate levels; no preservation caused high oxalate measurement, an analytical error, in their study.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
&amp;quot;The orange juice treatment was associated with higher urinary excretion of endogenously-derived oxalate, citrate, and calcium, and a higher urinary pH. Since these urinary changes were not observed during the supplemental ascorbate period, the two sources of ascorbate differentially affected key urinary components which are related to calcium oxalate nephrolithiasis.&amp;quot; (Liebman M., Weiwan C., Harvey E., and Boenisch L. 1996) http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/pdf&lt;br /&gt;
&lt;br /&gt;
6 healthy individuals&lt;br /&gt;
&lt;br /&gt;
24 hr&lt;br /&gt;
&lt;br /&gt;
175 mg unlabelled oxalate loads&lt;br /&gt;
&lt;br /&gt;
18 mg labelled oxalate loads 1,2-13C2&lt;br /&gt;
&lt;br /&gt;
vitamin C from orange juice&lt;br /&gt;
&lt;br /&gt;
vitamin C from supplemental form&lt;br /&gt;
&lt;br /&gt;
*** What did the subjects eat?&lt;br /&gt;
&lt;br /&gt;
**** Access to article is limited to Abstract. Open Access?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5018</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5018"/>
		<updated>2015-09-06T18:23:06Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Dumping Article Links on Vitamin C to Oxalate&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;The current page is a work in progress. &lt;br /&gt;
&lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
&lt;br /&gt;
Going to test Wiki&amp;#039;s ability to organize sources.&lt;br /&gt;
&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - aytundra.&lt;br /&gt;
&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
TO BEGIN&lt;br /&gt;
&lt;br /&gt;
DUMPING ARTICLE LINKS&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC39676/&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/8126804&lt;br /&gt;
&lt;br /&gt;
http://www.ncbi.nlm.nih.gov/pubmed/9589801&lt;br /&gt;
&lt;br /&gt;
http://www.nrjournal.com/article/S0271-5317%2897%2900012-2/abstract&lt;br /&gt;
&lt;br /&gt;
http://www.sciencedirect.com/science/article/pii/0005273681903126&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
http://www.medscape.com/viewarticle/556479_1&lt;br /&gt;
&lt;br /&gt;
http://www.2ndchance.info/oxalate-dogChai2005oxalatecontentfoods.pdf&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5017</id>
		<title>Oxalate</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Oxalate&amp;diff=5017"/>
		<updated>2015-09-06T18:11:43Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Intro to the page. Status = underconstruction&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;The current page is a work in progress. &lt;br /&gt;
Under construction = Sept 6, 2015 to Sept 30, 2015.&lt;br /&gt;
Just crashing this page as a place to put articles together.&lt;br /&gt;
Going to test Wiki&amp;#039;s ability to organize sources.&lt;br /&gt;
Disclaimer: Everything here is a rough draft, no real data, newbie working on Wiki(Sept 6, 2015). - aytundra.&lt;br /&gt;
Objective: Trying to piece together the articles on Vitamin C, Oxalate... to get a clearer picture.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=5014</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=5014"/>
		<updated>2015-07-03T21:58:28Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Questions for Tilapia page Broodstock&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloids, and some livestocks do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Nitrogen-fixation occurs in anaerobic(low oxygen) conditions:&lt;br /&gt;
&lt;br /&gt;
[i]Anabaena[/i]&amp;#039;s(cyanobacteria) heterocysts fixes nitrogen: http://link.springer.com/chapter/10.1007/0-306-48205-3_27&lt;br /&gt;
Anabaena growth and heterocysts: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845205/&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Heterocyst and Non-heterocysts, Nitrogen and Coral Reefs: http://www.nova.edu/ncri/11icrs/proceedings/files/mini-symposium%2019.pdf&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
===Questions===&lt;br /&gt;
&lt;br /&gt;
[quote=&amp;quot;www.waiwiki.org/index.php?title=Tilapia&amp;quot;&amp;quot;Broodstock&amp;quot;]&amp;quot;About 9 locations are available for marking. &lt;br /&gt;
By selecting 3 of these locations on each fish, a total of 84 combinations is possible. &lt;br /&gt;
(the first fish is marked 1,2,3, the second is marked 1,2,4 etc., and the last one is marked 6,7,8. &amp;quot;[/quote]&lt;br /&gt;
&lt;br /&gt;
Good Strategy using &amp;quot;combinations&amp;quot; from math; and eliminates need for multiple dyes.&lt;br /&gt;
but I am concerned about the locations of where you are marking the fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Why are you marking fish? &lt;br /&gt;
What is the purpose to identify each fish?&lt;br /&gt;
Is it on the tail or near the tail region? Pectoral fin?&lt;br /&gt;
Will the markings negatively affect fish growth and development?&lt;br /&gt;
Are the dyes non-toxic?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4883</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4883"/>
		<updated>2015-02-08T14:44:18Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spacing&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloids, and some livestocks do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Nitrogen-fixation occurs in anaerobic(low oxygen) conditions:&lt;br /&gt;
&lt;br /&gt;
[i]Anabaena[/i]&amp;#039;s(cyanobacteria) heterocysts fixes nitrogen: http://link.springer.com/chapter/10.1007/0-306-48205-3_27&lt;br /&gt;
Anabaena growth and heterocysts: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845205/&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Heterocyst and Non-heterocysts, Nitrogen and Coral Reefs: http://www.nova.edu/ncri/11icrs/proceedings/files/mini-symposium%2019.pdf&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Egg ==&lt;br /&gt;
Cooking reduced aminoacid contents in egg yolks. http://www.sciencedirect.com/science/article/pii/S0308814611006248&lt;br /&gt;
&lt;br /&gt;
Eggs treated with this method at lower temperatures could have a longer shelf life of up to 12 weeks. http://ps.oxfordjournals.org/content/91/6/1444&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4882</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4882"/>
		<updated>2015-02-08T14:43:49Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Egg&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloids, and some livestocks do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Nitrogen-fixation occurs in anaerobic(low oxygen) conditions:&lt;br /&gt;
&lt;br /&gt;
[i]Anabaena[/i]&amp;#039;s(cyanobacteria) heterocysts fixes nitrogen: http://link.springer.com/chapter/10.1007/0-306-48205-3_27&lt;br /&gt;
Anabaena growth and heterocysts: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845205/&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Heterocyst and Non-heterocysts, Nitrogen and Coral Reefs: http://www.nova.edu/ncri/11icrs/proceedings/files/mini-symposium%2019.pdf&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Egg ==&lt;br /&gt;
Cooking reduced aminoacid contents in egg yolks. http://www.sciencedirect.com/science/article/pii/S0308814611006248&lt;br /&gt;
Eggs treated with this method at lower temperatures could have a longer shelf life of up to 12 weeks. http://ps.oxfordjournals.org/content/91/6/1444&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4879</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4879"/>
		<updated>2015-02-05T17:49:22Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Heterocyst Anabaena&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloids, and some livestocks do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Nitrogen-fixation occurs in anaerobic(low oxygen) conditions:&lt;br /&gt;
&lt;br /&gt;
[i]Anabaena[/i]&amp;#039;s(cyanobacteria) heterocysts fixes nitrogen: http://link.springer.com/chapter/10.1007/0-306-48205-3_27&lt;br /&gt;
Anabaena growth and heterocysts: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845205/&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Heterocyst and Non-heterocysts, Nitrogen and Coral Reefs: http://www.nova.edu/ncri/11icrs/proceedings/files/mini-symposium%2019.pdf&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4876</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4876"/>
		<updated>2015-02-05T17:06:46Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Space formatting&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloids, and some livestocks do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Nitrogen-fixation occurs in anaerobic(low oxygen) conditions:&lt;br /&gt;
&lt;br /&gt;
[i]Anabaena[/i]&amp;#039;s(cyanobacteria) heterocysts fixes nitrogen: http://link.springer.com/chapter/10.1007/0-306-48205-3_27&lt;br /&gt;
&lt;br /&gt;
Heterocyst and Non-heterocysts, Nitrogen and Coral Reefs: http://www.nova.edu/ncri/11icrs/proceedings/files/mini-symposium%2019.pdf&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4874</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4874"/>
		<updated>2015-02-05T17:00:50Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Nitrogen-fixation and Anabaena&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloids, and some livestocks do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
Nitrogen-fixation occurs in anaerobic(low oxygen) conditions:&lt;br /&gt;
Heterocyst on Anabaena(anaerobic): http://link.springer.com/chapter/10.1007/0-306-48205-3_27&lt;br /&gt;
Heterocyst and Nitrogen and Coral Reefs: www.nova.edu/ncri/11icrs/proceedings/files/mini-symposium%2019.pdf&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4676</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4676"/>
		<updated>2015-01-23T20:23:18Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Other Silicon Sources?&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloid content, and some livestock do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Other Silicon Sources? == &lt;br /&gt;
Volcano ash, Obsidian glass, perlite water soluble, not heated diatomaceous earth (can it be recycled for soil use?), pumice stone.&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4675</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4675"/>
		<updated>2015-01-23T20:12:40Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Volcano Ash&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better. Helps maintain water content at the roots.&lt;br /&gt;
But some fungal symbionts, might contain alkaloid content, and some livestock do not tolerate alkaloids.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Volcano Ash == &lt;br /&gt;
Silicon Source?&lt;br /&gt;
Obsidian&lt;br /&gt;
Ecology of diatoms and volcano islands?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4644</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4644"/>
		<updated>2015-01-22T00:43:02Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: --&amp;gt; Fungal Symbionts&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Fungal Symbionts ==&lt;br /&gt;
Fungal symbionts on the root systems of plants can help plants grow better.&lt;br /&gt;
But some fungal symbionts, might contain alkaloid content, and some livestock do not tolerate alkaloids.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4643</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4643"/>
		<updated>2015-01-21T22:28:19Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: --&amp;gt;Vitellogenin&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Vitellogenin ==&lt;br /&gt;
What is Vitellogenin?&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin is found in:&lt;br /&gt;
&lt;br /&gt;
Insects insect&amp;#039;s yolk&lt;br /&gt;
&lt;br /&gt;
Chicken liver (of egg-laying hens)&lt;br /&gt;
&lt;br /&gt;
Rainbow Trout (female)&lt;br /&gt;
&lt;br /&gt;
Blackchin Tilapia&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin mitochondria RNA (VgR mRNA)&lt;br /&gt;
&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Chickens.&lt;br /&gt;
&lt;br /&gt;
Avian vitellogenin gene is expressed only in the liver of egg-laying hens. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Immature chicks or roosters can activate the avian vitellogenin gene by oestradiol. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Hormones activates the oestrogen response element (ERE) and not organ specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Demethylation of specific mCpGs in the promoter region occurs parallel to onset of transcription; the promoter region is hormone and expression specific. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
Another protein (protein in full text) binds with high affinity to the ERE. http://www.ncbi.nlm.nih.gov/pubmed/?term=1968660&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Pesticides.&lt;br /&gt;
&lt;br /&gt;
Pesticides (organochlorine) might increase vitellogenin content in [i]Sarotherodon melanotheron[/i] (blackchin tilapia). http://www.sciencedirect.com/science/article/pii/S0048969701010531&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Vitellogenin &amp;amp; Genistein. &lt;br /&gt;
&lt;br /&gt;
Genistein increased vitellogenin content in female rainbow trout [i]Oncorhynchus mykiss[/i], however, it might interfere with the endogenous ratio of estrogen/genistein, lowering the number of fish fertilized/hatched. http://www.sciencedirect.com/science/article/pii/S0016648000975853&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
What is Genistein? &lt;br /&gt;
&lt;br /&gt;
Genistein is a phytoestrogen, and occurs especially in soyabeans.http://www.sciencedirect.com/science/article/pii/S0278691505001912&lt;br /&gt;
{So feeding fish soya might not be a good idea?}&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4615</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4615"/>
		<updated>2015-01-20T13:02:23Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Vitellogenin&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
Insects might be a source of vitellogenin protein. Does it matter if it is &amp;quot;tilapia&amp;quot; vitellogenin protein or not?&lt;br /&gt;
- Vitellogenin (Vg)&lt;br /&gt;
- Vitellogening mitochondria RNA (VgR mRNA)&lt;br /&gt;
- Vitellogenin receptor (VgR)&lt;br /&gt;
- low-density lipoprotein receptor (LDLR)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4613</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4613"/>
		<updated>2015-01-14T20:23:48Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Brineshrimp&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
Quantity:&lt;br /&gt;
Approximately &amp;lt;5 liters of brine shrimp cysts were prepared at a time for zebrafish. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp might be a source of fats for fish. But the ratio and quality of fats might depend on what the brine shrimp was fed during the 24 hours post hatching of the shrimp. Emulsions of Vitamin C, Vitamin E were variables manipulated in their studies to enhance nutrient content of brine shrimp. http://www.sciencedirect.com/science/article/pii/S0044848601006986&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Fish size and feed type.&lt;br /&gt;
Tilapia Larvae:&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;br /&gt;
Adult Tilapia:&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Problems of Quantity: &lt;br /&gt;
How much food does a tilapia larvae need? &lt;br /&gt;
How much food of each type (algae, plankton, insects, shrimps,...etc.) does a tilapia larvae need?&lt;br /&gt;
How much food does it take to run a tilapia larvae nursery?&lt;br /&gt;
How much food does it take to run an adult tilapia farm?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4607</id>
		<title>Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4607"/>
		<updated>2015-01-14T14:54:20Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Feeding==&lt;br /&gt;
[[Image:Table_1.jpg|thumb|left| Table 1 for feeding tilapia [http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf © Alaa A. El-Dahhar]]]&lt;br /&gt;
[[Image:Chaoborus.jpg|thumb|right| Chaoborus [http://en.wikipedia.org/wiki/Glassworm#mediaviewer/File:GlasswormLateralView.JPG © Viridiflavus]]]&lt;br /&gt;
[[Image:Table_2.jpg|thumb|left| Table 2 for feeding tilapia [http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf © BFAR]]]&lt;br /&gt;
[[Image:Chironomus.jpg|thumb|right| Chironomus [http://www.thamesvalleybirds.co.uk/insect-invertebrate-macro-photographs/11156-non-biting-midge-male-probably-chironomus-plumosus.html © ladylouise62]]]&lt;br /&gt;
[[Image:Ceriodaphnia_reticulata.jpg|thumb|right| Water fleas [http://www.micromagus.net/microscopes/pondlife_cladocera.html © micromagus.net]]]&lt;br /&gt;
&lt;br /&gt;
Tilapia may survive on [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] or [http://www.waiwiki.org/index.php?title=Lemna_For_Fish duckweed] alone, but combined feeding results in higher growth rates. Feeding on algae. feeding most intensively occurs between 12.00 and 18.00, and in this time span the fish may consume over 3% of their bodyweight.[http://cabdirect.org/abstracts/20013100658.html;jsessionid=1F937711ACC14AC20505C4692DBC7CB3] Feed efficiency is highest at 2% BW.[http://www.sciencedirect.com/science/article/pii/S0044848697000392] Tilapia may also feed on insects / larvae [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2028.2004.00503.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false], such as water fleas (Ceriodaphnia &amp;lt; 1mm long) [http://link.springer.com/article/10.1007/BF00016658#page-1] (1&amp;gt; Daphnia &amp;lt;5 mm long; which cannot easily avoid predation) [http://www.int-res.com/articles/ame/25/a025p215.pdf] (or Daphniamenucoensis, which are halophylic), Chaoborus midge larva (&amp;lt; 2cm) and Chironomus midges.[http://www.nativefishlab.net/library/textpdf/17926.pdf] Superior growth is achieved by partial replacement with feed from animal origin, such as commercial fishfeed or insects (eg [http://www.waiwiki.org/index.php?title=Fruit_flies fruit flies]) and [http://www.waiwiki.org/index.php?title=Armyworm worms]. Water transparency contributes to the inclusion of insects in Nile tilapia diets.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Protein and energy of the animal-based foodstuffs are more available to Oreochromis niloticus than that of the plant-based foodstuffs.[http://www.sciencedirect.com/science/article/pii/0044848687902298] &lt;br /&gt;
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In outdoor tanks fecundity and final mean weight is higher in tilapia fed 3% bw compared to lower feeding rates.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.t01-1-00864.x/abstract] In adult tilapia, the optimal interval between feedings is 4 to 5 hours. At shorter intervals, eaten food will surpass the already filled stomach, and become waste. Fry may be fed 8 to 10 times a day.[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf] Energy retention is highest in juvenile Tilapia (av. 34 g.) fed 3 times daily. Protein retention is highest in fish fed 5 times daily.[https://evols.library.manoa.hawaii.edu/handle/10524/19120] Tilapia may fast up to a week with compensatory enhanced growth after refeeding.[http://www.tandfonline.com/doi/abs/10.1300/J028v18n03_02#.VEZlXvnV9JA]&lt;br /&gt;
&lt;br /&gt;
Table 1 (on the left) can be used by fish farmers to adjust feeding rate and frequency after determination of the hatchery constant by sampling and weighing the fish. The hatchery constant will depend on water quality, stocking density etc.[http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf]&lt;br /&gt;
Table 2 (on the left) is a schedule for the administration of commercial fish feed. Larger feed particle sizes are associated with lower fish growth rates.[http://www.sciencedirect.com/science/article/pii/S0044848610006332]&lt;br /&gt;
&lt;br /&gt;
==Protein==&lt;br /&gt;
&lt;br /&gt;
Protein requirement for maximum performance of tilapia larva is 35 -&amp;gt; 50%, decreasing with increasing fish size.[http://jn.nutrition.org/content/111/6/1001.long][http://www.pjbs.org/pjnonline/fin988.pdf][http://www.sciencedirect.com/science/article/pii/004484869290278S] In tilapia fry the feed conversion ratio and the highest protein growth rate are highest with a 45% crude protein diet, but the protein efficiency ratio and protein productive value was highest with the 25% crude protein diet. Fingerlings and advanced juveniles showed optimum growth performance with the 35% crude protein diet.[http://www.sciencedirect.com/science/article/pii/S0044848609008849] In young tilapia, out of artificial diets with dietary protein levels of 20%, 30%, 40% and 50%, best growth and food conversion efficiency was obtained with 40% dietary protein.[http://www.sciencedirect.com/science/article/pii/0044848688900075] Maximum food conversion efficiency and growth of young tilapia was obtained using the diet containing 30% protein (compared to 20%, 40% and 50% protein), decreasing with increasing protein levels.[http://www.sciencedirect.com/science/article/pii/0044848688900075]&lt;br /&gt;
Juvenile tilapia need 30-40% protein, while adult tilapia need 20-30% dietary protein for optimum performance.&lt;br /&gt;
Tilapia broodstock need 35-40% protein for optimum reproduction, spawning efficiency, and larval growth and survival.[http://www.sciencedirect.com/science/article/pii/S0044848696013658] [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1998.00206.x/abstract][http://www.sciencedirect.com/science/article/pii/S0044848602002211]. In tilapia average 62 g. weight and 18 cm long, there was no significant increase in growth rate with increasing dietary protein levels (from 25% to 30%).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Excess protein results in unionized ammonia in the environment.[http://www.sciencedirect.com/science/article/pii/S0044848609008849]&lt;br /&gt;
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Duckweeds grown in enriched water may contain 30 to 40% protein.[http://pubs.acs.org/doi/pdf/10.1021/jf60230a040] Wolffia arrhiza (dry weight) duckweed may contain up to 40% protein.[http://www.ncbi.nlm.nih.gov/pubmed/16232450] Lemna paucicostata dry mass protein contents may range from 26% to 45%.[http://www.sciencedirect.com/science/article/pii/0304377088900794] Lemna minor grown in swine lagoon wastewater contained 32% protein.[http://www.sciencedirect.com/science/article/pii/S0960852412012291]&lt;br /&gt;
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Green [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] (Chlorella vulgaris, Scenedesmus obliquus) and blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) may accumalate protein from 8 to 54% as available nitrogen increases. [http://www.sciencedirect.com/science/article/pii/S0031942200803040] Spirulina platensis contains 50 to 65% protein (10% is non-protein nitrogen)[http://pubs.acs.org/doi/abs/10.1021/jf00105a022], or even up to 71.9% protein.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2012.05303.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] At high salinities, protein levels in Spirulina decrease while carbohydrates increase as an adaptation for efficient osmoregulation. [http://link.springer.com/chapter/10.1007/0-306-46855-7_18]&lt;br /&gt;
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Insect true protein contents may be reasonably estimated via crude protein (nitrogen x 6.25).[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Crude protein content of feeder insects (housefly, fly larvae, cockroach nymphs, tebo worms) may range from 15.5 to 19.7% [http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Total crude protein in dry matter is 32.5% in earth worms and 64.4% in adult crickets (water content &amp;gt;50%)&lt;br /&gt;
Protein contents of Armyworm (Spodoptera exempta) moths are similar in gregarious and solitary moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756]&lt;br /&gt;
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Silkworm (Bombyx mori) pupae are a rich source of high quality protein.[http://www.sciencedirect.com/science/article/pii/S0278691506000147] In silkworm larvae, two storage proteins may account for 60% of total fat body protein (80% of the soluble protein) in females, compared to only 20% of the total fat body protein in males.[http://www.sciencedirect.com/science/article/pii/0020179080900244] &lt;br /&gt;
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Mealworms (Tenebrio molitor) from commercial source contained 22.3% protein.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae but contained 76% protein on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847] Mealworms contain 53% (Tenebrio molitor) and 45% (Zophobas morio; &amp;#039;Superworm&amp;#039;) protein in dry matter.[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0051145] (Egg production in Tenebrio molitor is 640 eggs / female / year, and 1500 eggs / female / year in Zophobas morio) Yellow mealworms (Tenebrio molitor; 4.8-182.7 mg) fed on wheat flour and brewers yeast contained 24.3-27.6% protein.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] A 40% protein fish meal diet with up to 40% replacement of fish meal with mealworm (Tenebrio molitor) meal does not affect growth of catfish. Catfish fed diets with up to 80% replacement of fish meal with the worm meal still displayed good growth and feed utilization efficiency. [http://onlinelibrary.wiley.com/doi/10.1046/j.1355-557x.2001.00024.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Cuticle larval / pupal mealworm proteins are more water-soluble at low temperatures (4 to 10°C) and become more hydrophobic at 25°C.[http://www.sciencedirect.com/science/article/pii/S0965174802000450] These cuticle proteins (as in the locust) lack acidic amino acid residues, methionine, cysteine and tryptophan.[http://www.sciencedirect.com/science/article/pii/096517489400048M]&lt;br /&gt;
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The digestibility coefficients for protein in Oreochromis niloticus are: soybean meal (91%), fish meal (86%), ground corn (83%), wheat middlings (75%), poultry-offal meal (74%), and brewers grain (63%).[http://www.sciencedirect.com/science/article/pii/0044848687902298]&lt;br /&gt;
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Protease activity in Tilapia is relatively low, in comparison to Grass carp (Ctenopharyngododn idellus), Common carp (Cyprinus carpiol), silver carp (Hypophthaimichthys molitrix) and bighead carp (Aristichys nobilis Richardson).[http://en.cnki.com.cn/Article_en/CJFDTOTAL-BEAR199302007.htm] Alkaline protease activity in Oreochromis niloticus is significantly inhibited by the ingestion of soybean meal, corn gluten meal and wheat bran, in comparison to the sensitivity to protease inhibitors in seabream and sole.[http://www.sciencedirect.com/science/article/pii/S0305049199000243]&lt;br /&gt;
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==Fat==&lt;br /&gt;
[[Image:Oreochromis_niloticus_1.jpg|thumb|left| Oreochromis niloticus [http://www.scandfish.com/ig/gallery.asp?action=viewimage&amp;amp;imageid=726&amp;amp;text=&amp;amp;categoryid=24&amp;amp;box=&amp;amp;shownew=]]]&lt;br /&gt;
[[Image:Oreochromis_niloticus_2.jpg|thumb|left| Oreochromis niloticus [http://www.fao.org/fishery/culturedspecies/Oreochromis_niloticus/en]]]&lt;br /&gt;
[[Image:Oreochromis_niloticus_3.jpg|thumb|left| Oreochromis niloticus [http://www.fishing-khaolak.com/fish_species/freshwater_species/nile_tilapia.html]]]&lt;br /&gt;
[[Image:Oreochromis_mossambicus_1.jpg|thumb|left| Oreochromis mossambicus [http://www.scandfish.com/ig/gallery.asp?action=viewimage&amp;amp;categoryid=24&amp;amp;text=&amp;amp;imageid=936&amp;amp;box=&amp;amp;shownew=]]]&lt;br /&gt;
[[Image:Oreochromis_mossambicus_4.jpg|thumb|left| Oreochromis mossambicus [http://www.fishing-khaolak.com/fish_species/freshwater_species/mozambique_tilapia.html]]]&lt;br /&gt;
[[Image:Oreochromis_mossambicus_2.jpg|thumb|left| Oreochromis mossambicus [http://mitofish.aori.u-tokyo.ac.jp/species/detail.html?genus=Oreochromis&amp;amp;species=mossambicus]]]&lt;br /&gt;
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Tilapia do well on fat versus carbohydrates as a source of energy.[http://www.ncbi.nlm.nih.gov/pubmed/22004562] Excess dietary lipids, however, are readily stored in the carcass and the viscera, but are not utilized for improving growth or food utilization efficiency.[http://www.sciencedirect.com/science/article/pii/004484869190224U]&lt;br /&gt;
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Larvae of mealworms, crickets, waxworms and fruit flies (Drosophila melanogaster) on average have 30% of dry matter higher fat content than adult species.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Newly emerged Armyworm moths may (still) contain high levels of (glyceride [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1993.tb00462.x/abstract]) lipid (largely in the abdominal), the quantity depends on the larval feeding conditions. Both the Armyworm larvae and young adult moths have the capacity to accumulate lipid reserves in excess. Big female moths contain relatively much fat. The moths are able to supplement their lipid reserves following carbohydrate uptake.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1986.tb00433.x/abstract] Gregarious larva have 2.5 to 6.1 higher abdominal glyceride contents than solitaria phase moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756] The stored fat may serve as fuel for long migratory flights.[http://www.sciencedirect.com/science/article/pii/0305049188901484] In feeder insects (house fly, fly larvae, cockroach nymphs, tebo worms), crude fat may range from 1.9% to 29.4%.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Yellow mealworms (4.8-182.7 mg) fed on wheat flour and brewers yeast contained 12.0-12.5% fat.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] Mealworms (Tenebrio molitor) from commercial source contained 15% fat.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae and contained 6.4% fat on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847]&lt;br /&gt;
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Energy content for silkworms is 674 kcal/kg and 2741 kcal/kg for waxworms.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10031/abstract]&lt;br /&gt;
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In regard to gross energy, the digestibilities in Oreochromis niloticus are: animal oil (93%), fish meal (80%), ground corn (76%), poultry-offal meal (59%), wheat middlings (58%), soybean meal (56%), and brewers grain (30%).[http://www.sciencedirect.com/science/article/pii/0044848687902298] Replacing sunflower oil (69% omega-6; 0% omega-3) with perilla oil (50 to 60% ALA/omega-3) changed fatty acid profiles in Nile tilapia, which stabilized after 20 days under the new diet. Total omega-3 in muscle tissue fat increased from 6.4% to 18.2% (almost 3-fold), particularly due to a 9.4 fold increase in ALA levels.[http://www.ncbi.nlm.nih.gov/pubmed/24262550] Palm oil may replace soybean oil in feeds for Oreochromis niloticus fingerlings without any negative effect on growth or body composition.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1996.tb00988.x/abstract]&lt;br /&gt;
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Spirulina may contain 7% [http://www.ejbiotechnology.info/content/vol9/issue4/full/5/] to 14% fat (of dry mass)[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1]. At low nitrogen (N) levels, green algae (Chlorella vulgaris and Scenedesmus obliquus) contain 45% lipids of biomass, containing mainly 16:0 and 18:1 fatty acids. At high N levels, total lipids dropped to 20% of the dry weight, with elevated levels of polyunsaturated C16 and C18 fatty acids [[http://www.sciencedirect.com/science/article/pii/S0031942200803040] during the initial stages of growth. Towards the end of growth the main lipids contained mainly saturated fatty acids (mostly 18:1 and 16:0).[http://www.sciencedirect.com/science/article/pii/S0031942200803052] In blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) lipid composition was reported not to be affected by N concentrations [http://www.sciencedirect.com/science/article/pii/S0031942200803040], but by sodium-nitrate as well as by temperature.[http://www.znaturforsch.com/ac/v59c/s59c0055.pdf] The fatty acids 18:3n−3, 18:2n−6, 18:0, 18:1 and 16:0 dominate in microalgae (eg Chlorella vulgaris, Scenedesmus abundans, Monoraphidium minitum), but are highest in Chlorella vulgaris. This difference is not reflected in their predator, Brachionus calyciflorus, and in Tilapia feeding on Brachionus calyciflorus. The Tilapia zillii larvae contained relatively much 22:6n−3, which is indicative for the capacity to elongate and desaturate dietary linoleic and linolenic acid.[http://www.sciencedirect.com/science/article/pii/S0044848699000137]&lt;br /&gt;
&lt;br /&gt;
==Carbohydrates==&lt;br /&gt;
&lt;br /&gt;
Tilapia is relatively well equipped (due to alpha-Amylases[http://www.ncbi.nlm.nih.gov/pubmed/11250550] produced by Aeromonas, Bacteroidaceae and Clostridium strains[http://www.ncbi.nlm.nih.gov/pubmed/9081303]) for utilizing carbohydrates.[http://www.ncbi.nlm.nih.gov/pubmed/23168215] Tilapia can utilize up to 46% dietary starch without growth retardation.[http://www.researchgate.net/publication/240652906_Effects_of_dietary_carbohydrate_level_on_growth_and_body_composition_of_juvenile_tilapia_Oreochromis_niloticusxO._aureus] Tilapias do better on starch than on glucose (even when the glucose diet is supplemented with chromic oxide) [http://www.ncbi.nlm.nih.gov/pubmed/7722702] Starch consists of amylose and amylopectin. Amylopectin is more susceptible to enzymatic cleavage. A high-dietary amylose-amylopectin ratio (&amp;gt; 0.24; as in corn starch) decreases starch digestibility and postprandial peak blood glucose and triglycerides, and inhibits feed efficiency and growth in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Amylopectin accounts for about 75% of the starch weight in reserve starch (in storage organs) [http://www.sciencedirect.com/science/article/pii/S1360138598013429] and typically more than 90% in transitory starch (in photosynthetic organs)[http://www.ncbi.nlm.nih.gov/pubmed/12068097/]. Spirodela polyrrhiza amylose content was 21%[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 25% in potatoes[http://link.springer.com/article/10.1007/BF02849767#page-1], 7-44% in barley[http://ajcn.nutrition.org/content/59/5/1075.short] and 25–28 in wheat.[http://www.sciencedirect.com/science/article/pii/S0924224405003572] Feeds with smaller granule size have higher starch digestibility than those with bigger granules. Spirodela polyrrhiza granules range from 1 to 8 μ [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 2-55 μ in wheat starch [http://link.springer.com/article/10.1023/A:1022255309597#page-1], 7 to 22 µ in corn starch and 8 to 110 µ in potatoes [http://www.karger.com/Article/Abstract/196856].&lt;br /&gt;
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Oreochromis niloticus weight gain, feed conversion, specific growth rate and protein efficiency ratio were improved with diets containing date (15%, 30% and 45%) as compared with the starch diet (0% date diet). The diet containing 30% date was superior to all other test diets in terms of all the above growth parameters, while body fat was reduced.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.00872.x/abstract] &lt;br /&gt;
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In tilapia, polysaccharides have antibacterial properties, increasing resistance to infections.[http://www.ncbi.nlm.nih.gov/pubmed/9280393] Spirulina platensis contains 13.6% carbohydrate, principally glucose along with rhamnose, mannose, xylose, galactose and two unusual sugars (2-O-methyl-L-rhamnose and 3-O-methyl-L-rhamnose).[http://www.sciencedirect.com/science/article/pii/S0031942200846981] Spirulina platensis contains various polysaccharides [http://www.morevollara.com/assets/downloads/two-Immunostimulatory-Activity-Pugh-2001.pdf], including starch.[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1] The amount of starch is low due to the high activity of α and β amylase in Spirulina.[http://www.ijpba.info/ijpba/index.php/ijpba/article/viewFile/894/607] In Spirulina platensis, the polysaccharide SP-4 consists of L-rhamnose, L-fucose, L-arabinose, D-glucose, D-glucoseamine and D-galacturonic acid. It enhances proliferation of lymphocytes.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GZHX199701006.htm] The polysaccharide PSP in Spirulina platensis is composed of L-fucose, D-mannose, D-galactose, D-glucose and glucuronic acid.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-DLYY503.001.htm] Another polysaccharide in Spirulina platensis contains glucose, principally, with small amounts of sulphate; a glucan backbone with glucosyl sidechains.[http://www.sciencedirect.com/science/article/pii/0308814689900198] Some Spirulina polysaccharides have antiviral properties.[http://online.liebertpub.com/doi/abs/10.1089/aid.1996.12.1463][https://www.jstage.jst.go.jp/article/cpb/49/1/49_1_108/_article] Spirulina platensis also secretes polysaccharides.[http://link.springer.com/article/10.1007/BF00138541#page-1] The cell wall of Spirulina platensis lacks cellulose (and is therefore readily digested).[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1]&lt;br /&gt;
&lt;br /&gt;
The cell walls of green algae (Chlorophyta) contain cellulose (and pectose, the outer layer) and carbohydrates are stored as starch. Mealworms (Tenebrio molitor) from commercial source contained 3.6% carbohydrates.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503]&lt;br /&gt;
&lt;br /&gt;
==Chito-oligosaccharides==&lt;br /&gt;
[[Image:scheme.jpg|thumb|left| Feeding scheme ]]&lt;br /&gt;
In tilapia stomach, intestine, and serum, chitinases are present. [http://www.ncbi.nlm.nih.gov/pubmed/17126584] Chitinases are enzymes required to enzymatically decompose chitin. Chitin is an amino polysaccharide; a long chain of many monosaccharide (N-acetylglucosamine) units. A chito-oligosaccharide is a short chain of just a few linked units of N-acetylglucosamine. Chitin may be split into chito-oligosaccharides. Chitin plus other composite materials (eg glycoproteins, calcium carbonate) forms the exoskeletons of insects and the flexible body wall of larva / caterpillars (eg silkworms[http://www.sciencedirect.com/science/article/pii/S0144861705005412] and fruit flies[http://www.ncbi.nlm.nih.gov/pubmed/807670]). Apparent digestibility coefficient of chitin ingested by Oreochromis niloticus from crayfish exoskeleton meal was 69.3%.[http://www.sciencedirect.com/science/article/pii/S0044848604007148] The peritrophic matrix of the tobacco hornworm (Manduca sexta) may contain 40% chitin.[http://www.sciencedirect.com/science/article/pii/0965174895000534] (Peritrophic matrices usually contain 3 to 13% chitin[http://jeb.biologists.org/content/206/24/4393.full]) Wild-caught earthworms may contain 51% chitin of dry matter.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Crustaceans may contain 12 to 20% chitin.[http://business.highbeam.com/435986/article-1G1-328419079/chitin-extraction-crustacean-shells-using-biological] Arthropod species (eg crustaceans, arachnids) with a chitin content of over 50% (of total weight) are eliminated unchanged by Tilapia zillii [http://www.sciencedirect.com/science/article/pii/0044848678900157] Insects may contain 0.3 to 5% chitin. (1.2 to 14% of dry matter)[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Chitin is also an ingredient of cell walls in algae.[http://www.ncbi.nlm.nih.gov/pubmed/3910139][http://www.ncbi.nlm.nih.gov/pubmed/23559820] Dietary intake of chito-oligosaccharides can improve intestinal health, and improve tilapia resistance to infection.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Dietary intake of chito-oligosaccharides at up to 4 g/kg may considerably improve growth, survival and immune response.[http://wenku.baidu.com/view/6da4fdd250e2524de5187ec7.html] Dietary intake of chito-oligosaccharides at 3 to 5 g/kg for juvenile GIFT tilapia may increase growth performance, nonspecific immunity and promote blood lipid metabolism.[http://www.agrpaper.com/applied-research-of-chito-oligosaccharide-on-gift-tilapia-oreochromis-niloticus.htm]&lt;br /&gt;
&lt;br /&gt;
==Vitamins &amp;amp; Minerals==&lt;br /&gt;
Oxalic acid is an anti-nutrient, reducing nutrient conversion in tilapia. High calcium available to duckweeds results in higher duckweed oxalic acid contents.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2004.00923.x/pdf] Lower duckweed-calcium may be compensated for by calcium-carbonate in the water, as freshwater fish take up Ca2+ predominantly through the gills [http://www.sbz.org.br/revista/artigos/66268.pdf], or by higher calcium in worms fed to tilapia. In mealworms supplemented with Ca from CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; (4 to 12%), the Ca content of the mealworms increased linearly with the Ca content of the substrate during the first 24 hours and decreased after over one week, especially at the higher levels of Ca supplementation. The Ca in these mealworms was 76% as bioavailable. [http://www.bioone.org/doi/abs/10.1638/1042-7260(2000)031%5B0512:ITCCOM%5D2.0.CO%3B2] Ca concentrations of silkworms also increased in a linear fashion with increasing levels of dietary Ca. Gut-loading diets for mealworms should be supplemented to contain 9% calcium, iron (51 mg/kg) and manganese (31 mg/kg). Gut-loading diets for silkworms should be supplemented to contain 23 g calcium per kg feed.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10082/abstract]&lt;br /&gt;
&lt;br /&gt;
Insects (mealworms, waxworms, crickets, fruitflies) on average have low calcium concentrations (0.11%), but sufficient concentrations of Cu, Fe, Mg, P, and Zn to meet known requirements of domestic birds and mammals. Supermealworms (Zophobas morio) and waxworms contain deficient levels of manganese. Earthworms meet all dietary mineral requirements. [http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract]&lt;br /&gt;
&lt;br /&gt;
Intestinal microorganisms in Tilapia nilotica produce at least 11.2 ng of vitamin B12 per g of body weight per day. In 16-weeks without Dietary B12, fish weight increased 8-fold (normal growth), and liver-stored B12 did not decrease.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1982)111%3C485%3AISADNO%3E2.0.CO%3B2#.VCvqCfl_s10] Magnesium absorption from the gastrointestinal tract may be highly efficient in Oreochromis mossambicus.[http://www.ncbi.nlm.nih.gov/pubmed/24194247]&lt;br /&gt;
&lt;br /&gt;
==Supplements==&lt;br /&gt;
[[Image:Allspice.jpg|thumb|right| Allspice [http://en.wikipedia.org/wiki/Allspice#mediaviewer/File:AllspiceBowl.JPG © Jonathunder]]]&lt;br /&gt;
[[Image:Babysbreath.jpg|thumb|right| Baby&amp;#039;s breath [http://en.wikipedia.org/wiki/Gypsophila_paniculata#mediaviewer/File:Gypsophila_paniculata.jpg © PiPi]]]&lt;br /&gt;
&lt;br /&gt;
* Saponin-rich plants such as Quillaja saponaria (Soap bark tree), yucca and Sapindus saponaria (wingleaf soapberry, western soapberry, jaboncillo) may increase growth, increase the nce male to female ratio in tilapia [http://www.ncbi.nlm.nih.gov/pubmed/22444893] and increase serum cholesterol in males.[http://www.sciencedirect.com/science/article/pii/S1532045602001679] Gypsophila paniculata (baby&amp;#039;s breath) and Quillaja saponaria (both high in saponins) may induce release of LH (Luteinizing hormone)[http://www.ncbi.nlm.nih.gov/pubmed/15792626] Supplementing Tilapia fry with 700 mg/kg extracted Quillaja saponin extract resulted in significantly increased growth, higher number of males, higher LH release and higher serum cholesterol.[http://www.ncbi.nlm.nih.gov/pubmed/12458187] Saponin supplementation diminishes egg production by females.[http://www.ncbi.nlm.nih.gov/pubmed/11423383] Intestinal magnesium uptake in tilapia is stimulated by chloride and inhibited by saponins (which may induce intravesicular magnesium accumulation.[http://www.ncbi.nlm.nih.gov/pubmed/8952951] In tilapia, saponins selectively permeabilize the plasma membrane.[http://www.ncbi.nlm.nih.gov/pubmed/24194248]&lt;br /&gt;
* Allspice Pimenta dioica (10 g/kg fish) acts as a growth promoter to improve feed utilization and weight gain in Mozambique Tilapia fry and acts an antimicrobial agent to enhance disease resistance during first feeding of fry.[http://www.ncbi.nlm.nih.gov/pubmed/25229484] Allspice (aka Jamaica pepper, myrtle pepper, pimenta, pimento, English pepper, newspice) is dried unripe berries of Pimenta dioica.&lt;br /&gt;
* Honey bee pollen (2.5% (w/v)) increases growth performance parameters, feed efficiency ratio and immunological, hematological and biochemical parameters in young Nile tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25086230]&lt;br /&gt;
* A supplemental mixture (at 0.5 to 2% w/w) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle may increase lysozyme, superoxide dismutase and peroxidase activity, decrease malondialdehyde levels and reduce mortality in Aeromonas hydrophila-challenged tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24925761] 20 days administration of 1% Astragalus root (Radix astragalin seu Hedysari) plus Angelica Root (Angelicae Sinensis) at a ratio of 5:1 (w/w) increased body weight of carp relative to controls.[http://www.ncbi.nlm.nih.gov/pubmed/15123322] Another study found no effect on growth by an mixture of Astragalus membranaceus, orange peel, hawthorn, pilose asiabell root indigowoad root, taraxacum and malt.[http://www.ncbi.nlm.nih.gov/pubmed/18378466] Astragalus (aka milkvetch, locoweed) belongs to the legume family Fabaceae. Dietary Astragalus polysaccharides supplementation may improve growth performance and immune parameters of tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24657260] Calycosin is a major isoflavonoid extracted from astragalus. It has shown to have proangiogenic effects.[http://www.ncbi.nlm.nih.gov/pubmed/21909574] Two isomeres extracted from Astragalus membranceus may stimulate proliferation in human fetal lung diploid fibroblast cells.[http://www.ncbi.nlm.nih.gov/pubmed/14659591] &lt;br /&gt;
* Survival in Streptococcus agalactiae challenged tilapia is increased by inclusion of 0.1% Sophora flavescens (root extract) in the diet.[http://www.ncbi.nlm.nih.gov/pubmed/23092731] Sophora flavescens (member of the Fabaceae family) roots contain quinolizidine alkaloids, including matrine, oxymatrine and lupeol. Matrine inhibits cell proliferation [http://www.ncbi.nlm.nih.gov/pubmed/24653570] (including proliferation of cancer cells)[http://www.ncbi.nlm.nih.gov/pubmed/24137393][http://www.ncbi.nlm.nih.gov/pubmed/24137358] Matrine prevents cardiac arrhythmias by inhibiting ouabain-induced calcium overload in guinea pigs.[http://www.ncbi.nlm.nih.gov/pubmed/24680622] Matrine has neurotoxic properties in Zebrafish (Danio rerio) larvae.[http://www.ncbi.nlm.nih.gov/pubmed/24911943]&lt;br /&gt;
&lt;br /&gt;
==Dissolved oxygen==&lt;br /&gt;
Fish take up oxygen through the limited gill surface area. At low dissolved oxygen (DO), more metabolic energy is used by the ventilatory system to maintain O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; uptake, which limits weight gain and growth.[http://www.sciencedirect.com/science/article/pii/0044848694902364] Feed intake and growth at 5.6 ppm (mg/L) DO is significantly higher than at 3.0 ppm, particularly in big fish.[http://www.sciencedirect.com/science/article/pii/S0044848608000021] For fish over 200 grams, feed intake continuous to increase over 3.0 ppm DO (with 5.5 ppm being the incipient DO), but digestibility is higher at suboptimal DO.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2011.02882.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
DO levels in ponds increase during the night due to vertical circulation. Organic materials consume oxygen during decomposition.[http://deepblue.lib.umich.edu/bitstream/handle/2027.42/27066/0000056.pdf?sequence=1] DO levels sharply decline after feeding. Dissolved oxygen levels should be maintained above 5.0 ppm for best growth. At DO levels between 3.0–5.0 ppm feeding should be reduced, and feeding should be stopped at DO levels below 3.0 ppm. Low DO increases the toxicity of ammonia (NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; unionized ammonia &amp;gt; 2.0 mg/L is lethal for tilapia, growth is inhibited below 1.0 mg/L). &lt;br /&gt;
[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf]&lt;br /&gt;
Under recirculated water conditions, mean body weight of Oreochromis aureus was higher at 6.5 ppm DO (mg/L), but food conversion ratio was best at 3.7 ppm DO.[http://www.sciencedirect.com/science/article/pii/0144860995000135]&lt;br /&gt;
Intermediate densities of phytoplankton produce higher dissolved oxygen concentrations. Dissolved oxygen levels may be raised by increasing algal growth, not necessarily by reducing algal biomass.[http://www.sciencedirect.com/science/article/pii/0044848688903572] DO concentrations at dawn is not related to manure input.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract] Low DO increases mortality due to Streptococcus infection.[http://www.sciencedirect.com/science/article/pii/S004484860000346X]&lt;br /&gt;
&lt;br /&gt;
==Salinity==&lt;br /&gt;
[[Image:Saisola_soda_3.jpg|thumb|left| Salsola soda [http://www.invasive.org/browse/detail.cfm?imgnum=5374910 © Joseph M. DiTomaso]]]&lt;br /&gt;
[[Image:Saisola_soda_2.jpg|thumb|right| Salsola soda [http://www.magicgardenseeds.com/SAL02 © MagicGardenSeeds]]]&lt;br /&gt;
[[Image:Saisola_kali_3.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_3.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Saisola_kali_2.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_2.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Limestone_1.jpg|thumb|left| Limestone [http://en.wikipedia.org/wiki/Limestone#mediaviewer/File:Limestone_Formation_In_Waitomo.jpg © Ingolfson]]]&lt;br /&gt;
[[Image:Limestone_2.jpg|thumb|right| Limestone [http://www.arthurharrisson.com/exploitation%20of%20limestone.html © A. Harrisson]]]&lt;br /&gt;
[[Image:Coquina.jpg|thumb|left| Coquina [http://en.wikipedia.org/wiki/Coquina#mediaviewer/File:Washington_Oaks_Gardens_coquina01.jpg © Ebyabe]]]&lt;br /&gt;
[[Image:Coquina_2.jpg|thumb|right| Coquina [http://coquina-zine.com/?page_id=724]]]&lt;br /&gt;
[[Image:Chalk.JPG|thumb|left| Chalk [http://en.wikipedia.org/wiki/File:Seale_Chalk_Pit_02.JPG © Lewis Hulbert]]]&lt;br /&gt;
[[Image:Lacustrine_tufa.jpg|thumb|left| Lacustrine tufa [https://www.pinterest.com/pin/207024914092886147/ © Susanne Janecke]]]&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis niloticus&amp;#039;&amp;#039; may grow better in 7.5 ppt than in 0 ppt salinity [http://www.sciencedirect.com/science/article/pii/S0044848605000888] and may fairly tolerate salinity up to 10 ppt [http://www.sciencedirect.com/science/article/pii/0044848685901024] Tilapia may tolerate 0.5% salinity (5 ppt).[http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf] &lt;br /&gt;
At 8 g / L (8 ppt) salinity adult Oreochromis niloticus may be stocked at 40 fish / m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;. At 15 g/L (15 ppt) salinity total production is more than halfed.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2006.01605.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Hatching success for eggs spawned by yearling females may be similar in freshwater (30.9%), 10 ppt (32.7%) and 15 ppt (36.9%), and considerably higher for eggs spawned at 5 ppt (51.6%). [http://www.sciencedirect.com/science/article/pii/0044848685900882] Energy required by Oreochromis niloticus for osmoregulation is the least in the absence of an osmotic gradient (11.6‰ salinity) [http://www.nrcresearchpress.com/doi/abs/10.1139/f69-277#.VEY_ufnV9JA] In freshwater, heating water to temperatures above 27°C is not justifiable, while at 18 or 36 ppt salinity, heating water to 32°C can maximize growth rates without lowering growth efficiency.[http://www.sciencedirect.com/science/article/pii/004484869390392C] An increase in salinity inhibits growth, compensated by higher temperatures. Juvenile Oreochromis niloticus may be grown similarly in 28°C and freshwater as in 32°C and 8 ppt salinity.[http://www.sciencedirect.com/science/article/pii/S0044848696013609]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis mossambicus&amp;#039;&amp;#039; tend to have lower growth rates than Oreochromis niloticus.&lt;br /&gt;
Oreochromis mossambicus is euryhaline and grows more rapidly in brackish water. (50% seawater)[http://www.ncbi.nlm.nih.gov/pubmed/11248987] Seawater salinity is 35 ppt. Oreochromis mossambicus reared in salt water grows significantly larger than fish reared in fresh water. [http://www.sciencedirect.com/science/article/pii/S1096495903002458] This may be due to salt secretion by highly activated chloride cells in branchial and opercular epithelia [http://www.bioone.org/doi/abs/10.2108/zsj.17.149] and/or a decrease in the resting metabolic rate (which decreased with increasing fish size).[http://www.sciencedirect.com/science/article/pii/0044848695010130] In freshwater, Oreochromis mossambicus prefers 32°C, and higher temperatures with increasing salinity.[http://onlinelibrary.wiley.com/doi/10.1111/j.1752-1688.1986.tb01876.x/abstract] Increasing dietary energy concentration increased mortality in Oreochromis mossambicus. The optimum protein to energy ratio for rapid growth, efficient feed conversion and maximum retention of protein and energy appears to be approximately 23.8 mg of protein per kJ energy.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1995.tb00935.x/abstract] Addition of 17α-methyltestosterone enhances its growth beyond that produced by masculinization alone.[http://www.sciencedirect.com/science/article/pii/0044848693903472] Of the administered 17α-methyltestosterone, less than 1% remains after 100 hours following withdrawal.[http://www.sciencedirect.com/science/article/pii/0044848683900959] Oreochromis mossambicus may cope with either high osmolality (564 mOsm/L), 10 pH or 40°C for several weeks.[http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.1989.tb00440.x/abstract]&lt;br /&gt;
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&amp;#039;&amp;#039;Oreochromis mossambicus&amp;#039;&amp;#039; (♂) &amp;#039;&amp;#039;× Oreochromis niloticus&amp;#039;&amp;#039; (♀) may produce 100% male offspring (&amp;#039;red hybrid tilapia&amp;#039;) [http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0471048267.html](p.363), but spawning frequency may be very low [http://www.sciencedirect.com/science/article/pii/S0044848605000888] and infiltration of parental broodstock by hybrids may be difficult in mass production.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1994.tb00743.x/abstract] (Thai) Red Tilapia has sexual growth dimorphism in which males grow significantly faster than females. Sex reversal may be induced by fluoxymesterone in 4 days old fry.[http://www.ist.cmu.ac.th/researchunit/pcrnc/paper/bio/8.pdf] In 12 ppt salinity, 5 to 12 days old Red Hybrid fry (stocked at 5,660 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;) supplemented with 60 mg 17αethynyltestosterone (ET)/kg diet for at least 7-14 days produces nearly all-male populations (94.3-98.1%).[http://www.tandfonline.com/doi/abs/10.1300/J028v02n01_02#.VKb0-iuG_QM] &lt;br /&gt;
Oreochromis mossambicus (♀) × Oreochromis niloticus (♂) hybrids are relatively salinity tolerant. During 2 months, fingerling growth was not significantly different in 0 ppt, 17 ppt and 32 ppt salinity.[http://onlinelibrary.wiley.com/doi/10.1111/j.1749-7345.2001.tb00930.x/abstract] Median Lethal Salinity (50% survival in 96 h after transfer from freshwater) ranged from 17.2‰ at 30 days post-hatching to 26.7‰ at 60 days post-hatching.[http://www.sciencedirect.com/science/article/pii/0044848685902200] This may be due to Morphological changes in the oesophageal epithelium.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1988.tb05352.x/abstract] The hybrid is superior to Oreochromis mossambicus at all salinities and to Oreochromis niloticus at salinities above 10 ppt.[http://www.sciencedirect.com/science/article/pii/S0044848605000888] This hybrid may be deprived of food during one week in a 8 weeks period without negatively affecting growth.[http://www.sciencedirect.com/science/article/pii/S0044848600003537] Due to starvation, the percentage of polyunsaturated fatty acids (PUFA) increases significantly in these hybrids.[http://www.sciencedirect.com/science/article/pii/S0044848697000355] Growth rate, conversion efficiency and maximum food consumption in Red Hybrid Tilapia may be optimal at 28°C [http://europepmc.org/abstract/MED/11767691] and in 0 to 15 ppt salinity.[http://new.medigraphic.com/cgi-bin/resumenMainI.cgi?IDARTICULO=5732]&lt;br /&gt;
&lt;br /&gt;
==Alkalinity &amp;amp; pH==&lt;br /&gt;
&lt;br /&gt;
Alkalinity is the &amp;quot;buffering&amp;quot; capacity of water (as containing carbonates and bicarbonates) to resist a change in pH (pH fluctuations). In general, culture waters with pH below 7.0 and total alkalinity below 50 mg /L will get benefits by liming.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] Liming is the application of high calcium-carbonate (and/or high magnesium-carbonate) rocks to the water. To increase pH, add sodium carbonate (Na&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;) or calcium carbonate (CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;). Sodium carbonate is more effective due to its higher solubility. Sodium carbonate may be extracted (as Barilla) from the ash of plants growing in sodium-rich soils, such as Halogeton sativus (aka Salsola sativa), Salsola soda and Salsola kali. The harvested plants are dried and subsequently burned. From this ash, the (water soluble) sodium carbonate is extracted with water. This solution is boiled dry, resulting in &amp;quot;barilla&amp;quot;.[http://en.wikipedia.org/wiki/Barilla] Calcium carbonate is the main component in snailshells and eggshells, and present in rocks such as limestone, chalk, marble, travertine and tufa. Limestone (mainly calcite and aragonite) is partially soluble in acid. Calcite water solubility decreases as temperature increases. Coquina is deposited shells (from molluscs etc) containing calcite and phosphate, and may be used as a fertilizer. It is soft and hardens by drying. Chalk is a porous sedimentary calcite rock, formed under water. Travertine forms from geothermal springs. Tufa (mainly calcite, plus some aragonite) is a soft, highly porous variety of limestone. It may mainly form in wetlands, at springs, waterfall cascades (fluvial tufas), and at the periphery of lakes (lacustrine tufa).&lt;br /&gt;
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To increase alkalinity, one may add sodium bicarbonate, coquina or limestone rocks, so that the water will dissolve large amounts of calcium and magnesium.[http://www.sbz.org.br/revista/artigos/66268.pdf] In Ghana, coquina may be found in the Akuse region (10 km south-east of Kpong)[http://cdn.intechweb.org/pdfs/26948.pdf], and along the Gulf of Guinea coast. Large limestone deposits can be found in Nauli (near Axim), Buipe, Bongo-Da (Northern) and Oterkpalu (near Koforidua), and minor deposits at Daboya (Northern), Anyaboni, Dedeso and Asuboni (next to Bawdua) in Eastern, the Ejura scarp (Ejurachem scarp in Ashanti), Kintampo and Prang (Brong Ahafo). [https://books.google.nl/books?id=U49Q8BgiarkC&amp;amp;pg=PA60&amp;amp;lpg=PA60&amp;amp;dq=bongo+da+limestone&amp;amp;source=bl&amp;amp;ots=YgBrXqgFIa&amp;amp;sig=2smEC4l_ctAclKe7sdS_IN9HBUY&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=tUm2VLbPNcvzap-ZgbAD&amp;amp;ved=0CDEQ6AEwBjgK#v=onepage&amp;amp;q=bongo%20da%20limestone&amp;amp;f=false]. To create sodium bicarbonate, sodium carbonate may be dissolved in water and treated with carbon dioxide. Solid sodium bicarbonate will precipitate. To increase the alkalinity of 10.000 L water with 10 ppm, add 168 g. of sodium bicarbonate.[http://www.expertpool.biz/ph_alkalinity.htm] Adding sodium carbonate or calcium carbonate decreases the level of free ammonium ions.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
In waters with algae (eg Spirulina), the algae will take up CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; for photosynthesis. As a result, less CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; reacts with H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O to form carbonic acid. This turns HCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt; (bicarbonate) and CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-2&amp;lt;/sup&amp;gt; (carbonate) ions into the&lt;br /&gt;
major forms of inorganic carbon, increasing alkalinity (and DO).[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2000.00493.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; may also be withdrawn (which results in an increase in pH) by adding (bi)carbonates. (Example formula: Na&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O =&amp;gt; 2NaHCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;)[http://books.google.nl/books?id=Sz4RYEyH5DsC&amp;amp;dq=Pond+aquaculture+water+quality+management&amp;amp;lr=&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] &lt;br /&gt;
&lt;br /&gt;
The appropriate levels for aquaculture are 20-150 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L alkalinity and 7.4-8.2 pH.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
Adding sodium carbonate to water with 20 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity, increasing alkalinity up to 77 meq/L, does not affect &amp;#039;&amp;#039;Oreochromis niloticus&amp;#039;&amp;#039; growth rates. Calcium carbonate is better for tilapia growth, as increasing water hardness as well.[http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/download/8510/8510] Total hardness refers to the content of soluble Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; and Mg&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; in the water. Tilapia need to absorb these ions from the gills for several physiological functions. When total alkalinity is much greater than hardness, water pH may reach 11 in the mid-afternoon due to photosynthesis, because more free CO&amp;lt;sup&amp;gt;2-&amp;lt;/sup&amp;gt;&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; remains in solution.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] Acidifying processes decrease alkalinity, but not hardness of the water.&lt;br /&gt;
&lt;br /&gt;
Fish stocked at higher densities may show better survival rates when total water alkalinity is increased from 30 to 80 or 130 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; per L.[http://onlinelibrary.wiley.com/doi/10.1111/j.1749-7345.2007.00118.x/abstract] Water alkalinity of 35 to 53 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L water may be optimum for larval tilapia growth.[http://www.ablimno.org.br/acta/pdf/acta_limnologica_contents1604E_files/Art4_16(4).pdf] For actual optimum alkalinity levels for Oreochromis niloticus growth, the total hardness/total alkalinity ratio seems to be key and needs to be greater than 1.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] Growth of male sex reversed juvenile Oreochromis niloticus is higher at 146 mg/L calciumcarbonate than at 82 mg/L (the difference is actually in calciumcarbonate). A simultaneous increase in water hardness and alkalinity (due to the application of CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;) induces better growth than only an increase in alkalinity. The best conditions for Oreochromis niloticus growth are 7.4-8.2 pH; total alkalinity &amp;gt; 50 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L; calcium hardness &amp;gt; 140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;/L and free CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; &amp;lt; 7 mg/L, [http://eduem.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6263] though free CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; in water, even when in high concentrations, seems to be harmless to fish when there are suitable concentrations of dissolved oxygen in water.[http://onlinelibrary.wiley.com/doi/10.1111/j.1749-7345.2000.tb00889.x/abstract] To lower a too high pH, one may add CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; (or duckweed) to high-DO water. &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis mossambicus&amp;#039;&amp;#039; may thrive in water with 245 mg/L bicarbonates [http://www.tandfonline.com/doi/abs/10.1080/07438140709354036#.VKriGyuG_QM] or 289 mg/L total alkalinity and 8.8 to 9.4 pH [http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1981)110%3C239%3ADAAOPD%3E2.0.CO%3B2#.VKwPHyuG_QM][http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1977.tb05129.x/abstract][http://www.sciencedirect.com/science/article/pii/0883292796000339] or 7.9 to 8.6 pH (dominant fork length is 24 cm)[http://aciar.gov.au/files/node/329/pr98_pdf_69082.pdf#page=66], and may cope with 10 pH for several weeks.[http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.1989.tb00440.x/abstract]&lt;br /&gt;
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==Water flow==&lt;br /&gt;
A flow rate of 0.5 L/min per kg biomass appears to be desirable for intensive culture of tilapia.[http://www.sciencedirect.com/science/article/pii/004484869190073G]&lt;br /&gt;
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==Stocking density==&lt;br /&gt;
Tilapia needs to be stocked by age group to prevent cannabalism. The risk of cannabalism increases with increasing size differences.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA465&amp;amp;dq=algae+tilapia&amp;amp;ots=BVjAbOF-zV&amp;amp;sig=aEIMIY5ekRp5adhtpwsOIqPQTMQ#v=onepage&amp;amp;q=algae%20tilapia&amp;amp;f=false] Given reasonable assumptions about production costs, the optimal final density of Oreochromis niloticus in a tank-based flow-through system is 73.7 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.ncbi.nlm.nih.gov/pubmed/23915501]&lt;br /&gt;
Combined net yield of both caged and open-pond tilapia was highest in the treatment with 50 large fish (av. 141 g.) per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.sciencedirect.com/science/article/pii/S0044848696013774]&lt;br /&gt;
Broodstock may be best stocked at 4 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1999.00311.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
Red tilapia stocked in a pulsed-flow aquaculture system at densities of 10 and 20 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; had significantly higher individual weights than fish stocked at 30, 50, and 70 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;, due to limiting water quality; ammonia concentrations may be high (4 mg/L), and dissolved oxygen low (2 mg/L).[http://www.tandfonline.com/doi/abs/10.1300/J028v05n01_08#.VEY9SvnV9JA] Oreochromis niloticus fry may grow optimally when stocked at 5 fry / L and fed at 30% bodyweight / day.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2002.00700.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Oreochromis niloticus fingerlings may be stocked at a density of 42.6/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; with a production of 18–20 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; in 210 days at a water flow rate of 1 L/min/kg fish biomass.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1989.tb00440.x/abstract] With dissolved oxygen levels below 2 ppm (or even below 1 ppm) most of the time, stocking 3 fish per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; was more profitable due to increased mortality and lower growth rates at higher stocking densities (6 and 9 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;), despite similar water qualities.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/487.pdf]&lt;br /&gt;
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==Growth==&lt;br /&gt;
Tilapia nilotica fingerlings grow optimally on diets containing 30-40% protein, 12-15% lipids, and 30-40% digestible carbohydrate.[http://ir.kagoshima-u.ac.jp/bitstream/10232/15661/1/AN00181784_v6-1_p56-71.pdf]&lt;br /&gt;
Where extra protein (from 25% to 30%) did not increase growth in young tilapia (av. 18 cm long, 62 g. weight), increasing feeding levels to 2% of bodyweight / day did.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
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juvenile Oreochromis niloticus grow better at 26 and 30°C, than at 22 and 34°C.[http://www.sciencedirect.com/science/article/pii/S0306456507001118] Feed efficiency and protein efficiency ratio are better and juvenile fish grow better at 28°C than at 22°C and 34°C. Liver gluconeogenesis and lipogenesis are increased by low temperature (22°C).[http://www.ncbi.nlm.nih.gov/pubmed/24556257] Growth and feed utilization efficiency is similar in genetically improved farmed tilapia (GIFT), genetically male Nile tilapia (GMNT) and conventional Nile tilapia (CNT).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2007.01679.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Large Oreochromis niloticus (av. 288 g.) grow less in recirculating aquaculture systems compared to flow-through systems. The opposite is true for small fish (av. 81 g.)[http://www.sciencedirect.com/science/article/pii/S0044848609008254]&lt;br /&gt;
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The use of tuna by-product meal as a total replacement for fish meal into tilapia diets increases oxidative stress.[http://www.ncbi.nlm.nih.gov/pubmed/25139749]&lt;br /&gt;
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Higher energy conservation is achieved when fish are exposed to longer rather than shorter photoperiod cycles.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00450.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
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If reproduction can be delayed in the females, average growth rates comparable to those of an all-male population may be achieved.&lt;br /&gt;
[http://www.sciencedirect.com/science/article/pii/004484869390034V]&lt;br /&gt;
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==Strains==&lt;br /&gt;
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Compared to Oreochromis niloticus strains from Egypt and Senegal (bred from wild collected stocks), and ‘Israel’, ‘Singapore’, ‘Taiwan’ and ‘Thailand’ (strains maintained for aquaculture purposes), a strain bred in Ghana from wild collected stocks showed a significantly lower body weight at 210 days.&lt;br /&gt;
[http://www.sciencedirect.com/science/article/pii/004484869390034V]&lt;br /&gt;
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Triploidy may be induced in Oreochromis niloticus by heat shock. No differences in growth between triploids and diploid controls may be observed at age of maturation, but triploids are significant heavier (P &amp;lt; 0.01) than control fish at the end of the experiment. Triploid males exceeded body weights of respective controls on average by 66 ± 17%, whereas triploid females displayed an even higher increase in body weight compared to diploid ones (95 ± 27%).[http://www.sciencedirect.com/science/article/pii/0044848695011048]&lt;br /&gt;
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==Masculinization==&lt;br /&gt;
Estrogen synthesis is crucial for ovarian differentiation, and transcription of the aromatase gene can be proposed as a key step in that process in fish. Treatments with an aromatase inhibitor (ATD, 1,4,6- androstatriene-3-17-dione) result in 75.3% masculinization of an all-female (XX) population in tilapia (dosage 150 mg/kg of food). The effectiveness of the aromatase inhibition by ATD is demonstrated by the marked decrease of the gonadal aromatase activity in treated animals versus control.[http://www.ncbi.nlm.nih.gov/pubmed/10471475]&lt;br /&gt;
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Aromatase activity as a key factor in sexual differentiation in Oreochromis niloticus.[http://www.ncbi.nlm.nih.gov/pubmed/11424212] The most sensitive time to aromatase inhibitors lies in the first week (between 7 and 14 days post hatch). Treatment with the aromatase inhibitor Fadrozole (nonsteroidal) showed a dose-dependent increase in the percentage of males from 0 to 200 mg per kg. At higher doses (200 to 500 mg / kg), the percentage of males remained more or less constant (92.5-96.0%).[http://www.ncbi.nlm.nih.gov/pubmed/10861549]&lt;br /&gt;
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The masculinizing actions of 17alpha-methyltestosterone (MT) are most potent at up to day 20 of age.[http://www.ncbi.nlm.nih.gov/pubmed/11694764] Treating female tilapia Oreochromis niloticus with methyltestosterone (at a dose of 50 mcg/g diet) resulted in 100% masculinization.[http://www.ncbi.nlm.nih.gov/pubmed/16115634] Fry may be fed a commercial diet (SRT-95) with 30 ppm α-methyltestosterone for 21 days to yield 99% males.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA183&amp;amp;dq=stocking+density+oreochromis+niloticus+flow-through&amp;amp;ots=BVjC9QG1uV&amp;amp;sig=oZEoAvtMefOYOjPjqXQ-tbd_z_w#v=onepage&amp;amp;q&amp;amp;f=false] Numerous papers have reported that adding 17α-methyltestosterone at 60 mg/kg to the diet for several weeks induces mono-sex male populations. During growing-out season, muscle testosterone concentration in monosex tilapia was sharply decreased to 2.625 ± 0.303 ng/g at the harvesting date. [http://www.ejabf.eg.net/pdf/Vol-17-n-2/Issue%206.pdf]&lt;br /&gt;
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Treatment with tamoxifen and letrozole (200mg/kg feed) to fingerlings of O. niloticus for 60 days brought about 98.5% masculinization.&lt;br /&gt;
Treatment with 17α methyltestosterone (35 mg/kg feed) to fingerlings of O. mossambicus after 8 days post hatch for 60 days obtained 100% sex reversed males with excellent growth.[http://www.ncbi.nlm.nih.gov/pubmed/23063432]&lt;br /&gt;
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During the restricted developmental period temperature is of great influence. The critical period for elevated-temperature-induced masculinization lies between days 10 and 15 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] Higher temperatures (during 5 days) before they are 5 days old induces deformities. Masculinization is induced at elevated temperatures (28 to 32°C) during 5 days after 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577] The advantage of producing faster growing males of Nile tilapia at high temperature would hardly compensate the loss of production incurred during the masculinising treatment.[http://www.sciencedirect.com/science/article/pii/S004484860000452X] High-temperature (almost 36.90 °C) treatments yield a significantly higher proportion of males (64.20–80%) with lower survival rates (60–81%). The sex ratio of progenies reared at temperature below 36°C never deviated significantly from the balanced sex ratio.[http://www.sciencedirect.com/science/article/pii/S0306456507001118]&lt;br /&gt;
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==Feminization==&lt;br /&gt;
The critical period for low-temperature-induced feminization lies between days 5 and 10 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] The period of maximal feminizing action of 17beta-estradiol (E(2)) upon sex ratio is before 10 days posthatching in tilapia (Oreochromis mossambicus).[http://www.ncbi.nlm.nih.gov/pubmed/11694764]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. Higher temperatures (during 5 days) before they are 5 days old induces deformities. Gonadal feminization is induced at lower temperatures (20°C) for 5 days before 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577]&lt;br /&gt;
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==Parasites==&lt;br /&gt;
Tilapia may be infected with: &lt;br /&gt;
&lt;br /&gt;
* Nematodes / roundworms, such as Contracaecum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Contracaecum multipapillatum [http://www.ncbi.nlm.nih.gov/pubmed/24573461]), Paracamallanus cyathopharynx, Procamallanus laevionchus [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Rhabdochona esseniae [http://www.ncbi.nlm.nih.gov/pubmed/7596571], Rhabdochona paski [http://www.ncbi.nlm.nih.gov/pubmed/23595492], Gnathostoma spinigerum, Gnathostoma doloresi and Gnathostoma hispidum.[http://www.ajtmh.org/content/58/3/316.long] Human gnathostomiasis infection is related to ingestion of &amp;quot;ceviche&amp;quot;.[http://www.ncbi.nlm.nih.gov/pubmed/2588073]&lt;br /&gt;
* Cestodes (aka tapeworms), such as Amirthalingamia sp. (eg Amirthalingamia macracantha; higher prevalence and intensity in wet season [http://www.ncbi.nlm.nih.gov/pubmed/21972071]), and Cyclustera sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Cyclustera magna [http://www.ncbi.nlm.nih.gov/pubmed/15357392]) &lt;br /&gt;
* Trematodes / flukes (encysted metacercariae)[http://www.ncbi.nlm.nih.gov/pubmed/19795754], such as Clonorchis sinensis (human liver trematode)[http://www.ncbi.nlm.nih.gov/pubmed/22471793], Tylodelphys sp.[http://www.ncbi.nlm.nih.gov/pubmed/24407916], Stictodora tridactyla (in brackish-water fish)[http://www.ncbi.nlm.nih.gov/pubmed/2332641], Ribeiroia marini [http://www.ncbi.nlm.nih.gov/pubmed/24996], Clinostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] ((eg Clinostomum Complanatum, Clinostomum tilapiae, Euclinostomum hetereostomum [http://www.ncbi.nlm.nih.gov/pubmed/23113215]), Diplostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Neascus sp, Acanthostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Bolbophorus sp.[http://www.ncbi.nlm.nih.gov/pubmed/21972071], Haplorchis yokogawai [http://www.ncbi.nlm.nih.gov/pubmed/21073146], Haplorchis pumilio, Haplorchis taichui, Centrocestus formosanus, Stellantchas musfalcatus, Echinochasmus japonicus [http://www.ncbi.nlm.nih.gov/pubmed/18564743], Heterophyes heterophyes, Heterophyes aequalis, Pygidiopsis genata, Stictodora sp., Phagicola sp.[http://www.ncbi.nlm.nih.gov/pubmed/18339484] (eg Phagicola ascolonga[http://www.ncbi.nlm.nih.gov/pubmed/21073146]) and Prohemostomumn vivax.[http://www.ncbi.nlm.nih.gov/pubmed/18383799] These trematodes loose their viability after 48 hours at -5°C.[http://www.ncbi.nlm.nih.gov/pubmed/19795748] The conditions of gas supersaturation (gas pressure &amp;gt; 111.2% saturation; N supersaturation, O2 undersaturation) may result in heavy monogenetic trematode infections in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/9234554] In Thailand, fish-borne zoonotic trematode (FZT) metacercarial infections were found in Nile tilapia from cage (2.5%) and pond aquaculture systems (10%) and in wild caught fish.(53%) [http://www.ncbi.nlm.nih.gov/pubmed/24029716] In China, tilapia from nursery and grow-out ponds were sampled from monoculture, polyculture and integrated aquaculture systems, revealing a 1.5% prevalence of FZT infections (Heterophyidae and Echinostomatidae); lower than in wild caught fish. Integrated systems using animal manure and latrine wastes as fertilizer did not show a higher prevalence.[http://www.ncbi.nlm.nih.gov/pubmed/24018184] In Vietnam, the overall FZT prevalence in tilapia from wastewater ponds was 2.0%, but much higher in tilapia from farm ponds. [http://www.ncbi.nlm.nih.gov/pubmed/22471793]&lt;br /&gt;
* Monogenea (very small flatworms), such as Dactylogyrus minutus [http://www.ncbi.nlm.nih.gov/pubmed/24407916], Neobenedenia melleni [http://www.ncbi.nlm.nih.gov/pubmed/7707197][http://www.ncbi.nlm.nih.gov/pubmed/1597779], Enterogyrus cichlidarum (associated with overcrowding [http://www.ncbi.nlm.nih.gov/pubmed/7472888] and infection intensity increases with (tilapia) host size[http://www.ncbi.nlm.nih.gov/pubmed/2242958]), Gyrodactylus malalai (ectoparasite)[http://www.ncbi.nlm.nih.gov/pubmed/22807048], Gyrodactylus ergensi [http://www.ncbi.nlm.nih.gov/pubmed/19838735], Scutogyrus longicornis [http://www.ncbi.nlm.nih.gov/pubmed/25054495] and of the genus Cichlidogyrus [http://www.ncbi.nlm.nih.gov/pubmed/22436463], such as Cichlidogyrus tilapiae, Cichlidogyrus sclerosus [http://www.ncbi.nlm.nih.gov/pubmed/21791155], Cichlidogyrus dossoui [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Cichlidogyrus halli [[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Cichlidogyrus thurstonae [http://www.ncbi.nlm.nih.gov/pubmed/14707506], Cichlidogyrus berradae, Cichlidogyrus revesati, Cichlidogyrus legendrei and Cichlidogyrus lemoallei.[http://www.ncbi.nlm.nih.gov/pubmed/14535345] The highest parasite number was recorded in reservoir-dwelling, and lowest in stream-dwelling tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21791155] Gyrodactylus niloticus infection may particularly become lethal (in tilapia) when followed by exposure to the bacterial pathogen Streptococcus iniae.[http://www.ncbi.nlm.nih.gov/pubmed/17394525] Low stocking density and low water temperature may result in lower monogenea parasitism rate.[http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Acanthocephala (thorny-headed worms), such as Acanthogyrus (Acanthosentis) tilapiae.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] and Polyacanthorhynchus kenyensis [http://www.ncbi.nlm.nih.gov/pubmed/12015820] In Kenya, infection rates in wild Tilapia zillii ranged from 4.1 to 77.7%.[http://www.ncbi.nlm.nih.gov/pubmed/9332980] &lt;br /&gt;
* Ciliatea (&amp;#039;small flagella&amp;#039;), such as Epistylis sp.[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Paratrichodina africana [http://www.ncbi.nlm.nih.gov/pubmed/23731856], Trichodina compacta [http://www.ncbi.nlm.nih.gov/pubmed/21755153], Trichodina magna [[http://www.ncbi.nlm.nih.gov/pubmed/25054495] and Ichthyophthirius multifiliis.[http://www.ncbi.nlm.nih.gov/pubmed/22311586] Ichthyophthirius multifiliis load increases susceptibility and mortality of tilapia to Streptococcus iniae (Gram-positive bacteria) infection.[http://www.ncbi.nlm.nih.gov/pubmed/19750806] &lt;br /&gt;
* Dinophyceae (Dinoflagellata; &amp;quot;red tide&amp;quot;), such as Piscinoodinium pillulare.[http://www.ncbi.nlm.nih.gov/pubmed/25054495] (a dominant parasite in Brasilian tilapia ponds)[http://www.ncbi.nlm.nih.gov/pubmed/21755153] &lt;br /&gt;
* Myxosporea, such as Henneguya suprabranchiae [http://www.ncbi.nlm.nih.gov/pubmed/18516619], Myxobolus heterosporus [http://www.ncbi.nlm.nih.gov/pubmed/15819436] (induces total destruction of the intestine structure [http://www.ncbi.nlm.nih.gov/pubmed/12911060]), Myxobolus nounensis [http://www.ncbi.nlm.nih.gov/pubmed/11031757], Myxobolus dossoui (host size effect), Myxobolus zillii (seasonal pattern) [http://www.ncbi.nlm.nih.gov/pubmed/11383569], Myxobolus microcapsularis [http://www.int-res.com/articles/dao/44/d044p217.pdf], Myxobolus agolus, Myxobolus brachysporus, Myxobolus clarii, Myxobolus cichlidarum, Myxobolus tilapiae and Myxobolus camerounensis.[http://www.ncbi.nlm.nih.gov/pubmed/19069850]&lt;br /&gt;
* Conoidasida (unicellular), such as Goussia cichlidarum.[http://www.ncbi.nlm.nih.gov/pubmed/17885764]&lt;br /&gt;
* Phyllopharyngea (single-cell parasites), such as Chilodonella hexasticha.[http://www.ncbi.nlm.nih.gov/pubmed/22902259]&lt;br /&gt;
* Trhypochthoniellus longisetus longisetus, a mite.[http://www.ncbi.nlm.nih.gov/pubmed/21553570]&lt;br /&gt;
* Lamproglena sp. (Lernaeidae; small crustaceans) [http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Pentasomida (tongue worms; tiny crustaceans), such as Leiperia cincinnalis [http://www.ncbi.nlm.nih.gov/pubmed/9809320], Subtriquetra wedli [http://www.ncbi.nlm.nih.gov/pubmed/10192834] and Subtriquetra riley.[http://www.ncbi.nlm.nih.gov/pubmed/9809320]&lt;br /&gt;
* Anodontites trapesialis (the larval stage of this mussel).[http://www.ncbi.nlm.nih.gov/pubmed/12048579]&lt;br /&gt;
&lt;br /&gt;
No parasite vaccines exist.[http://www.ncbi.nlm.nih.gov/pubmed/15757476] Tilapia are relatively resistant to parasitic infections.[http://www.ncbi.nlm.nih.gov/pubmed/18537032] The prevalence of heterophyid encysted metacercariae infection decreases as fish size increases.[http://www.ncbi.nlm.nih.gov/pubmed/20644958] When dissolved oxygen, temperature, pH, nitrite and ammonia are within normal rate parasites may cause little harm to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19500461] Parasitic infection by Acanthogyrus tilapiae provokes an aggressive host response.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] Tilapia may produce an induced humoral immune response against Cichlidogyrus sp.[http://www.ncbi.nlm.nih.gov/pubmed/18564741] Oreochromis mossambicus initiated a strong immune protection by direct exposure with even a small number of parasites.[http://www.ncbi.nlm.nih.gov/pubmed/21739314] Gyrodactylus numbers fluctuate independently of temperature. In anticipation of immune defenses reaching the fish surface through mucus, Gyrodactylids (Monogenea) worms progressively move away from fins with high mucus cell density to those with low density.[http://www.ncbi.nlm.nih.gov/pubmed/21840127] Tilapia may also develop resistance to Neobenedenia sp. (Monogenea) infection.[http://www.ncbi.nlm.nih.gov/pubmed/21381523]&lt;br /&gt;
&lt;br /&gt;
Freshwater mollusks in tilapia ponds, such as Melania tuberculata, Melanoides turricula, Pomacea flagellata, Haitia cubensis and Anodontiles luteola, may host various parasites that are harmful to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21250483] The Biomphalaria sp. snails are often found in tilapia ponds (fresh water reservoirs are their natural habitat[http://www.ncbi.nlm.nih.gov/pubmed/11100444]) and are intermediates for Schistosoma mansoni.[http://www.scielo.br/scielo.php?script=sci_arttext&amp;amp;pid=S0074-02762001000900008&amp;amp;lng=en&amp;amp;nrm=iso&amp;amp;tlng=en] Biomphalaria cf. havanensis is an intermediate host for Diplostomum ompactum, causing higher levels of infections in cultured tilapia than wild tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19452167] The snail Bulinus truncatus is an intermediate host for Clinostomum tilapiae, Euclinostomum heterostomum, Bolbophorus levantinus and Neascus-type metacercariae. The snail Melanoides tuberculata is an intermediate host for Centrocestus sp. and Haplorchis sp. The snail Melanopsis costata is an intermediate host for Pygidiopsis genata, Phagicola longa.[http://www.ncbi.nlm.nih.gov/pubmed/12911062] The snail Thiara granifera is commonly infected with Haplorchis pumilio.[http://www.ncbi.nlm.nih.gov/pubmed/12015833] Dogs, cats and pigs may also be intermediate hosts for metacercariae.[http://www.ncbi.nlm.nih.gov/pubmed/18564743]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4598</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4598"/>
		<updated>2015-01-12T19:23:06Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: running thoughts&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia (Oreochromis Mossambicus) fed with these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;br /&gt;
Food, growth and dpf (days post fertilization) for Tilapia larvae?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4585</id>
		<title>Research Sandbox</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Research_Sandbox&amp;diff=4585"/>
		<updated>2015-01-09T19:11:13Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Brine Shrimp&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Brine shrimp ==&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp [i]Artemia [/i] spp.&lt;br /&gt;
&lt;br /&gt;
Brine shrimp are small crustaceans.&lt;br /&gt;
&lt;br /&gt;
Freshly prepared brine shrimp cysts (eggs) may be used to feed fish.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts and Zebrafish ([i]Danio rerio[/i]):&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts fed to adult zebrafish [i]Danio rerio[/i] encourages mating.&lt;br /&gt;
When brine shrimp were administered a few days before breeding,  the belly enlarges noticeably in adult female zebrafish.&lt;br /&gt;
Belly enlargement means that the male and female zebrafish are ready to be placed together in a small tank for mating.&lt;br /&gt;
Brine shrimp cysts can be fed occasionally to flake-fed fish as a live-feed for a broader nutrient range, so it is not only for breeding purposes.&lt;br /&gt;
&lt;br /&gt;
Zebrafish when consuming brine shrimp cysts will bite into it, releasing the reddish pink orangy gold contents, along with the salt. They do not eat the shell, so a few hours later you may have to check the tank and scoop out the waste. It is a much enjoyed treat by zebrafish. While feeding brine shrimp to fish, it might be nice to check the water quality and water salinity more frequently to maintain a nice tank for the fish. Note: Brine shrimp cysts should be given to fish size that can tolerate the size, tiny larval zebrafish were not given brine shrimp.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Brine shrimp cysts for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Brine shrimp may be fed to tilapia larvae.&lt;br /&gt;
Brine shrimp that consumed yeast, yeast expressing a tilapia vitellogenin protein (rVtg), contained vitellogenin; and larval tilapia fed these vitellogenin enriched brine shrimp grew better than without the vitellogenin enrichment; Brine shrimp without vitellogenin protein were also an acceptable feed for tilapia larvae http://www.ncbi.nlm.nih.gov/pubmed/?term=15735086.&lt;br /&gt;
This means that vitellogenin protein might be important to tilapia larval fish development.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp for Tilapia ([i]Oreochromis[/i])?:&lt;br /&gt;
&lt;br /&gt;
Adult Brine Shrimp fed with some dinoflagellates can sometimes contain dinoflagellate toxins such as [i]Gambierdiscus toxicus[/i]; and Tilapia fed with these toxin filled brine shrimp displayed behavioural abnormalities. http://link.springer.com/article/10.1007/BF00005871#page-1&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Are Brine Shrimp Cysts better than Adult Brine Shrimp for Tilapia feed?&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4537</id>
		<title>Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4537"/>
		<updated>2015-01-06T18:36:36Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Feeding==&lt;br /&gt;
[[Image:Table_1.jpg|thumb|left| Table 1 for feeding tilapia [http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf © Alaa A. El-Dahhar]]]&lt;br /&gt;
[[Image:Chaoborus.jpg|thumb|right| Chaoborus [http://en.wikipedia.org/wiki/Glassworm#mediaviewer/File:GlasswormLateralView.JPG © Viridiflavus]]]&lt;br /&gt;
[[Image:Table_2.jpg|thumb|left| Table 2 for feeding tilapia [http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf © BFAR]]]&lt;br /&gt;
[[Image:Chironomus.jpg|thumb|right| Chironomus [http://www.thamesvalleybirds.co.uk/insect-invertebrate-macro-photographs/11156-non-biting-midge-male-probably-chironomus-plumosus.html © ladylouise62]]]&lt;br /&gt;
[[Image:Ceriodaphnia_reticulata.jpg|thumb|right| Water fleas [http://www.micromagus.net/microscopes/pondlife_cladocera.html © micromagus.net]]]&lt;br /&gt;
&lt;br /&gt;
Tilapia may survive on [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] or [http://www.waiwiki.org/index.php?title=Lemna_For_Fish duckweed] alone, but combined feeding results in higher growth rates. Feeding on algae. feeding most intensively occurs between 12.00 and 18.00, and in this time span the fish may consume over 3% of their bodyweight.[http://cabdirect.org/abstracts/20013100658.html;jsessionid=1F937711ACC14AC20505C4692DBC7CB3] Feed efficiency is highest at 2% BW.[http://www.sciencedirect.com/science/article/pii/S0044848697000392] Tilapia may also feed on insects / larvae [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2028.2004.00503.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false], such as water fleas (Ceriodaphnia &amp;lt; 1mm long) [http://link.springer.com/article/10.1007/BF00016658#page-1] (1&amp;gt; Daphnia &amp;lt;5 mm long; which cannot easily avoid predation) [http://www.int-res.com/articles/ame/25/a025p215.pdf] (or Daphniamenucoensis, which are halophylic), Chaoborus midge larva (&amp;lt; 2cm) and Chironomus midges.[http://www.nativefishlab.net/library/textpdf/17926.pdf] Superior growth is achieved by partial replacement with feed from animal origin, such as commercial fishfeed or insects (eg [http://www.waiwiki.org/index.php?title=Fruit_flies fruit flies]) and [http://www.waiwiki.org/index.php?title=Armyworm worms]. Water transparency contributes to the inclusion of insects in Nile tilapia diets.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Protein and energy of the animal-based foodstuffs are more available to Oreochromis niloticus than that of the plant-based foodstuffs.[http://www.sciencedirect.com/science/article/pii/0044848687902298] &lt;br /&gt;
&lt;br /&gt;
In outdoor tanks fecundity and final mean weight is higher in tilapia fed 3% bw compared to lower feeding rates.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.t01-1-00864.x/abstract] In adult tilapia, the optimal interval between feedings is 4 to 5 hours. At shorter intervals, eaten food will surpass the already filled stomach, and become waste. Fry may be fed 8 to 10 times a day.[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf] Energy retention is highest in juvenile Tilapia (av. 34 g.) fed 3 times daily. Protein retention is highest in fish fed 5 times daily.[https://evols.library.manoa.hawaii.edu/handle/10524/19120] Tilapia may fast up to a week with compensatory enhanced growth after refeeding.[http://www.tandfonline.com/doi/abs/10.1300/J028v18n03_02#.VEZlXvnV9JA]&lt;br /&gt;
&lt;br /&gt;
Table 1 (on the left) can be used by fish farmers to adjust feeding rate and frequency after determination of the hatchery constant by sampling and weighing the fish. The hatchery constant will depend on water quality, stocking density etc.[http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf]&lt;br /&gt;
Table 2 (on the left) is a schedule for the administration of commercial fish feed. Larger feed particle sizes are associated with lower fish growth rates.[http://www.sciencedirect.com/science/article/pii/S0044848610006332]&lt;br /&gt;
&lt;br /&gt;
==Protein==&lt;br /&gt;
&lt;br /&gt;
Protein requirement for maximum performance of tilapia larva is 35 -&amp;gt; 50%, decreasing with increasing fish size.[http://jn.nutrition.org/content/111/6/1001.long][http://www.pjbs.org/pjnonline/fin988.pdf][http://www.sciencedirect.com/science/article/pii/004484869290278S] In tilapia fry the feed conversion ratio and the highest protein growth rate are highest with a 45% crude protein diet, but the protein efficiency ratio and protein productive value was highest with the 25% crude protein diet. Fingerlings and advanced juveniles showed optimum growth performance with the 35% crude protein diet.[http://www.sciencedirect.com/science/article/pii/S0044848609008849] In young tilapia, out of artificial diets with dietary protein levels of 20%, 30%, 40% and 50%, best growth and food conversion efficiency was obtained with 40% dietary protein.[http://www.sciencedirect.com/science/article/pii/0044848688900075] Maximum food conversion efficiency and growth of young tilapia was obtained using the diet containing 30% protein (compared to 20%, 40% and 50% protein), decreasing with increasing protein levels.[http://www.sciencedirect.com/science/article/pii/0044848688900075]&lt;br /&gt;
Juvenile tilapia need 30-40% protein, while adult tilapia need 20-30% dietary protein for optimum performance.&lt;br /&gt;
Tilapia broodstock need 35-40% protein for optimum reproduction, spawning efficiency, and larval growth and survival.[http://www.sciencedirect.com/science/article/pii/S0044848696013658] [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1998.00206.x/abstract][http://www.sciencedirect.com/science/article/pii/S0044848602002211]. In tilapia average 62 g. weight and 18 cm long, there was no significant increase in growth rate with increasing dietary protein levels (from 25% to 30%).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Excess protein results in unionized ammonia in the environment.[http://www.sciencedirect.com/science/article/pii/S0044848609008849]&lt;br /&gt;
&lt;br /&gt;
Duckweeds grown in enriched water may contain 30 to 40% protein.[http://pubs.acs.org/doi/pdf/10.1021/jf60230a040] Wolffia arrhiza (dry weight) duckweed may contain up to 40% protein.[http://www.ncbi.nlm.nih.gov/pubmed/16232450] Lemna paucicostata dry mass protein contents may range from 26% to 45%.[http://www.sciencedirect.com/science/article/pii/0304377088900794] Lemna minor grown in swine lagoon wastewater contained 32% protein.[http://www.sciencedirect.com/science/article/pii/S0960852412012291]&lt;br /&gt;
&lt;br /&gt;
Green [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] (Chlorella vulgaris, Scenedesmus obliquus) and blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) may accumalate protein from 8 to 54% as available nitrogen increases. [http://www.sciencedirect.com/science/article/pii/S0031942200803040] Spirulina platensis contains 50 to 65% protein (10% is non-protein nitrogen)[http://pubs.acs.org/doi/abs/10.1021/jf00105a022], or even up to 71.9% protein.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2012.05303.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
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Insect true protein contents may be reasonably estimated via crude protein (nitrogen x 6.25).[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Crude protein content of feeder insects (housefly, fly larvae, cockroach nymphs, tebo worms) may range from 15.5 to 19.7% [http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Total crude protein in dry matter is 32.5% in earth worms and 64.4% in adult crickets (water content &amp;gt;50%)&lt;br /&gt;
Protein contents of Armyworm (Spodoptera exempta) moths are similar in gregarious and solitary moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756]&lt;br /&gt;
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Silkworm (Bombyx mori) pupae are a rich source of high quality protein.[http://www.sciencedirect.com/science/article/pii/S0278691506000147] In silkworm larvae, two storage proteins may account for 60% of total fat body protein (80% of the soluble protein) in females, compared to only 20% of the total fat body protein in males.[http://www.sciencedirect.com/science/article/pii/0020179080900244] &lt;br /&gt;
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Mealworms (Tenebrio molitor) from commercial source contained 22.3% protein.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae but contained 76% protein on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847] Mealworms contain 53% (Tenebrio molitor) and 45% (Zophobas morio; &amp;#039;Superworm&amp;#039;) protein in dry matter.[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0051145] (Egg production in Tenebrio molitor is 640 eggs / female / year, and 1500 eggs / female / year in Zophobas morio) Yellow mealworms (Tenebrio molitor; 4.8-182.7 mg) fed on wheat flour and brewers yeast contained 24.3-27.6% protein.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] A 40% protein fish meal diet with up to 40% replacement of fish meal with mealworm (Tenebrio molitor) meal does not affect growth of catfish. Catfish fed diets with up to 80% replacement of fish meal with the worm meal still displayed good growth and feed utilization efficiency. [http://onlinelibrary.wiley.com/doi/10.1046/j.1355-557x.2001.00024.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Cuticle larval / pupal mealworm proteins are more water-soluble at low temperatures (4 to 10°C) and become more hydrophobic at 25°C.[http://www.sciencedirect.com/science/article/pii/S0965174802000450] These cuticle proteins (as in the locust) lack acidic amino acid residues, methionine, cysteine and tryptophan.[http://www.sciencedirect.com/science/article/pii/096517489400048M]&lt;br /&gt;
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The digestibility coefficients for protein in Oreochromis niloticus are: soybean meal (91%), fish meal (86%), ground corn (83%), wheat middlings (75%), poultry-offal meal (74%), and brewers grain (63%).[http://www.sciencedirect.com/science/article/pii/0044848687902298]&lt;br /&gt;
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Protease activity in Tilapia is relatively low, in comparison to Grass carp (Ctenopharyngododn idellus), Common carp (Cyprinus carpiol), silver carp (Hypophthaimichthys molitrix) and bighead carp (Aristichys nobilis Richardson).[http://en.cnki.com.cn/Article_en/CJFDTOTAL-BEAR199302007.htm] Alkaline protease activity in Oreochromis niloticus is significantly inhibited by the ingestion of soybean meal, corn gluten meal and wheat bran, in comparison to the sensitivity to protease inhibitors in seabream and sole.[http://www.sciencedirect.com/science/article/pii/S0305049199000243]&lt;br /&gt;
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==Fat==&lt;br /&gt;
[[Image:Oreochromis_niloticus_1.jpg|thumb|left| Oreochromis niloticus [http://www.scandfish.com/ig/gallery.asp?action=viewimage&amp;amp;imageid=726&amp;amp;text=&amp;amp;categoryid=24&amp;amp;box=&amp;amp;shownew=]]]&lt;br /&gt;
[[Image:Oreochromis_niloticus_2.jpg|thumb|left| Oreochromis niloticus [http://www.fao.org/fishery/culturedspecies/Oreochromis_niloticus/en]]]&lt;br /&gt;
[[Image:Oreochromis_niloticus_3.jpg|thumb|left| Oreochromis niloticus [http://www.fishing-khaolak.com/fish_species/freshwater_species/nile_tilapia.html]]]&lt;br /&gt;
[[Image:Oreochromis_mossambicus_1.jpg|thumb|left| Oreochromis mossambicus [http://www.scandfish.com/ig/gallery.asp?action=viewimage&amp;amp;categoryid=24&amp;amp;text=&amp;amp;imageid=936&amp;amp;box=&amp;amp;shownew=]]]&lt;br /&gt;
[[Image:Oreochromis_mossambicus_4.jpg|thumb|left| Oreochromis mossambicus [http://www.fishing-khaolak.com/fish_species/freshwater_species/mozambique_tilapia.html]]]&lt;br /&gt;
[[Image:Oreochromis_mossambicus_2.jpg|thumb|left| Oreochromis mossambicus [http://mitofish.aori.u-tokyo.ac.jp/species/detail.html?genus=Oreochromis&amp;amp;species=mossambicus]]]&lt;br /&gt;
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Tilapia do well on fat versus carbohydrates as a source of energy.[http://www.ncbi.nlm.nih.gov/pubmed/22004562] Excess dietary lipids, however, are readily stored in the carcass and the viscera, but are not utilized for improving growth or food utilization efficiency.[http://www.sciencedirect.com/science/article/pii/004484869190224U]&lt;br /&gt;
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Larvae of mealworms, crickets, waxworms and fruit flies (Drosophila melanogaster) on average have 30% of dry matter higher fat content than adult species.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Newly emerged Armyworm moths may (still) contain high levels of (glyceride [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1993.tb00462.x/abstract]) lipid (largely in the abdominal), the quantity depends on the larval feeding conditions. Both the Armyworm larvae and young adult moths have the capacity to accumulate lipid reserves in excess. Big female moths contain relatively much fat. The moths are able to supplement their lipid reserves following carbohydrate uptake.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1986.tb00433.x/abstract] Gregarious larva have 2.5 to 6.1 higher abdominal glyceride contents than solitaria phase moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756] The stored fat may serve as fuel for long migratory flights.[http://www.sciencedirect.com/science/article/pii/0305049188901484] In feeder insects (house fly, fly larvae, cockroach nymphs, tebo worms), crude fat may range from 1.9% to 29.4%.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Yellow mealworms (4.8-182.7 mg) fed on wheat flour and brewers yeast contained 12.0-12.5% fat.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] Mealworms (Tenebrio molitor) from commercial source contained 15% fat.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae and contained 6.4% fat on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847]&lt;br /&gt;
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Energy content for silkworms is 674 kcal/kg and 2741 kcal/kg for waxworms.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10031/abstract]&lt;br /&gt;
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In regard to gross energy, the digestibilities in Oreochromis niloticus are: animal oil (93%), fish meal (80%), ground corn (76%), poultry-offal meal (59%), wheat middlings (58%), soybean meal (56%), and brewers grain (30%).[http://www.sciencedirect.com/science/article/pii/0044848687902298] Replacing sunflower oil (69% omega-6; 0% omega-3) with perilla oil (50 to 60% ALA/omega-3) changed fatty acid profiles in Nile tilapia, which stabilized after 20 days under the new diet. Total omega-3 in muscle tissue fat increased from 6.4% to 18.2% (almost 3-fold), particularly due to a 9.4 fold increase in ALA levels.[http://www.ncbi.nlm.nih.gov/pubmed/24262550] Palm oil may replace soybean oil in feeds for Oreochromis niloticus fingerlings without any negative effect on growth or body composition.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1996.tb00988.x/abstract]&lt;br /&gt;
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Spirulina may contain 7% [http://www.ejbiotechnology.info/content/vol9/issue4/full/5/] to 14% fat (of dry mass)[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1]. At low nitrogen (N) levels, green algae (Chlorella vulgaris and Scenedesmus obliquus) contain 45% lipids of biomass, containing mainly 16:0 and 18:1 fatty acids. At high N levels, total lipids dropped to 20% of the dry weight, with elevated levels of polyunsaturated C16 and C18 fatty acids [[http://www.sciencedirect.com/science/article/pii/S0031942200803040] during the initial stages of growth. Towards the end of growth the main lipids contained mainly saturated fatty acids (mostly 18:1 and 16:0).[http://www.sciencedirect.com/science/article/pii/S0031942200803052] In blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) lipid composition was reported not to be affected by N concentrations [http://www.sciencedirect.com/science/article/pii/S0031942200803040], but by sodium-nitrate as well as by temperature.[http://www.znaturforsch.com/ac/v59c/s59c0055.pdf] The fatty acids 18:3n−3, 18:2n−6, 18:0, 18:1 and 16:0 dominate in microalgae (eg Chlorella vulgaris, Scenedesmus abundans, Monoraphidium minitum), but are highest in Chlorella vulgaris. This difference is not reflected in their predator, Brachionus calyciflorus, and in Tilapia feeding on Brachionus calyciflorus. The Tilapia zillii larvae contained relatively much 22:6n−3, which is indicative for the capacity to elongate and desaturate dietary linoleic and linolenic acid.[http://www.sciencedirect.com/science/article/pii/S0044848699000137]&lt;br /&gt;
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==Carbohydrates==&lt;br /&gt;
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Tilapia is relatively well equipped (due to alpha-Amylases[http://www.ncbi.nlm.nih.gov/pubmed/11250550] produced by Aeromonas, Bacteroidaceae and Clostridium strains[http://www.ncbi.nlm.nih.gov/pubmed/9081303]) for utilizing carbohydrates.[http://www.ncbi.nlm.nih.gov/pubmed/23168215] Tilapia can utilize up to 46% dietary starch without growth retardation.[http://www.researchgate.net/publication/240652906_Effects_of_dietary_carbohydrate_level_on_growth_and_body_composition_of_juvenile_tilapia_Oreochromis_niloticusxO._aureus] Tilapias do better on starch than on glucose (even when the glucose diet is supplemented with chromic oxide) [http://www.ncbi.nlm.nih.gov/pubmed/7722702] Starch consists of amylose and amylopectin. Amylopectin is more susceptible to enzymatic cleavage. A high-dietary amylose-amylopectin ratio (&amp;gt; 0.24; as in corn starch) decreases starch digestibility and postprandial peak blood glucose and triglycerides, and inhibits feed efficiency and growth in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Amylopectin accounts for about 75% of the starch weight in reserve starch (in storage organs) [http://www.sciencedirect.com/science/article/pii/S1360138598013429] and typically more than 90% in transitory starch (in photosynthetic organs)[http://www.ncbi.nlm.nih.gov/pubmed/12068097/]. Spirodela polyrrhiza amylose content was 21%[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 25% in potatoes[http://link.springer.com/article/10.1007/BF02849767#page-1], 7-44% in barley[http://ajcn.nutrition.org/content/59/5/1075.short] and 25–28 in wheat.[http://www.sciencedirect.com/science/article/pii/S0924224405003572] Feeds with smaller granule size have higher starch digestibility than those with bigger granules. Spirodela polyrrhiza granules range from 1 to 8 μ [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 2-55 μ in wheat starch [http://link.springer.com/article/10.1023/A:1022255309597#page-1], 7 to 22 µ in corn starch and 8 to 110 µ in potatoes [http://www.karger.com/Article/Abstract/196856].&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus weight gain, feed conversion, specific growth rate and protein efficiency ratio were improved with diets containing date (15%, 30% and 45%) as compared with the starch diet (0% date diet). The diet containing 30% date was superior to all other test diets in terms of all the above growth parameters, while body fat was reduced.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.00872.x/abstract] &lt;br /&gt;
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In tilapia, polysaccharides have antibacterial properties, increasing resistance to infections.[http://www.ncbi.nlm.nih.gov/pubmed/9280393] Spirulina platensis contains 13.6% carbohydrate, principally glucose along with rhamnose, mannose, xylose, galactose and two unusual sugars (2-O-methyl-L-rhamnose and 3-O-methyl-L-rhamnose).[http://www.sciencedirect.com/science/article/pii/S0031942200846981] Spirulina platensis contains various polysaccharides [http://www.morevollara.com/assets/downloads/two-Immunostimulatory-Activity-Pugh-2001.pdf], including starch.[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1] The amount of starch is low due to the high activity of α and β amylase in Spirulina.[http://www.ijpba.info/ijpba/index.php/ijpba/article/viewFile/894/607] In Spirulina platensis, the polysaccharide SP-4 consists of L-rhamnose, L-fucose, L-arabinose, D-glucose, D-glucoseamine and D-galacturonic acid. It enhances proliferation of lymphocytes.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GZHX199701006.htm] The polysaccharide PSP in Spirulina platensis is composed of L-fucose, D-mannose, D-galactose, D-glucose and glucuronic acid.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-DLYY503.001.htm] Another polysaccharide in Spirulina platensis contains glucose, principally, with small amounts of sulphate; a glucan backbone with glucosyl sidechains.[http://www.sciencedirect.com/science/article/pii/0308814689900198] Some Spirulina polysaccharides have antiviral properties.[http://online.liebertpub.com/doi/abs/10.1089/aid.1996.12.1463][https://www.jstage.jst.go.jp/article/cpb/49/1/49_1_108/_article] Spirulina platensis also secretes polysaccharides.[http://link.springer.com/article/10.1007/BF00138541#page-1] The cell wall of Spirulina platensis lacks cellulose (and is therefore readily digested).[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1]&lt;br /&gt;
&lt;br /&gt;
The cell walls of green algae (Chlorophyta) contain cellulose (and pectose, the outer layer) and carbohydrates are stored as starch. Mealworms (Tenebrio molitor) from commercial source contained 3.6% carbohydrates.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503]&lt;br /&gt;
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==Chito-oligosaccharides==&lt;br /&gt;
&lt;br /&gt;
In tilapia stomach, intestine, and serum, chitinases are present. [http://www.ncbi.nlm.nih.gov/pubmed/17126584] Chitinases are enzymes required to enzymatically decompose chitin. Chitin is an amino polysaccharide; a long chain of many monosaccharide (N-acetylglucosamine) units. A chito-oligosaccharide is a short chain of just a few linked units of N-acetylglucosamine. Chitin may be split into chito-oligosaccharides. Chitin plus other composite materials (eg glycoproteins, calcium carbonate) forms the exoskeletons of insects and the flexible body wall of larva / caterpillars (eg silkworms[http://www.sciencedirect.com/science/article/pii/S0144861705005412] and fruit flies[http://www.ncbi.nlm.nih.gov/pubmed/807670]). Apparent digestibility coefficient of chitin ingested by Oreochromis niloticus from crayfish exoskeleton meal was 69.3%.[http://www.sciencedirect.com/science/article/pii/S0044848604007148] The peritrophic matrix of the tobacco hornworm (Manduca sexta) may contain 40% chitin.[http://www.sciencedirect.com/science/article/pii/0965174895000534] (Peritrophic matrices usually contain 3 to 13% chitin[http://jeb.biologists.org/content/206/24/4393.full]) Wild-caught earthworms may contain 51% chitin of dry matter.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Crustaceans may contain 12 to 20% chitin.[http://business.highbeam.com/435986/article-1G1-328419079/chitin-extraction-crustacean-shells-using-biological] Arthropod species (eg crustaceans, arachnids) with a chitin content of over 50% (of total weight) are eliminated unchanged by Tilapia zillii [http://www.sciencedirect.com/science/article/pii/0044848678900157] Insects may contain 0.3 to 5% chitin. (1.2 to 14% of dry matter)[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Chitin is also an ingredient of cell walls in algae.[http://www.ncbi.nlm.nih.gov/pubmed/3910139][http://www.ncbi.nlm.nih.gov/pubmed/23559820] Dietary intake of chito-oligosaccharides can improve intestinal health, and improve tilapia resistance to infection.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Dietary intake of chito-oligosaccharides at up to 4 g/kg may considerably improve growth, survival and immune response.[http://wenku.baidu.com/view/6da4fdd250e2524de5187ec7.html] Dietary intake of chito-oligosaccharides at 3 to 5 g/kg for juvenile GIFT tilapia may increase growth performance, nonspecific immunity and promote blood lipid metabolism.[http://www.agrpaper.com/applied-research-of-chito-oligosaccharide-on-gift-tilapia-oreochromis-niloticus.htm]&lt;br /&gt;
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==Vitamins &amp;amp; Minerals==&lt;br /&gt;
Oxalic acid is an anti-nutrient, reducing nutrient conversion in tilapia. High calcium available to duckweeds results in higher duckweed oxalic acid contents.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2004.00923.x/pdf] Lower duckweed-calcium may be compensated for by calcium-carbonate in the water, as freshwater fish take up Ca2+ predominantly through the gills [http://www.sbz.org.br/revista/artigos/66268.pdf], or by higher calcium in worms fed to tilapia. In mealworms supplemented with Ca from CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; (4 to 12%), the Ca content of the mealworms increased linearly with the Ca content of the substrate during the first 24 hours and decreased after over one week, especially at the higher levels of Ca supplementation. The Ca in these mealworms was 76% as bioavailable. [http://www.bioone.org/doi/abs/10.1638/1042-7260(2000)031%5B0512:ITCCOM%5D2.0.CO%3B2] Ca concentrations of silkworms also increased in a linear fashion with increasing levels of dietary Ca. Gut-loading diets for mealworms should be supplemented to contain 9% calcium, iron (51 mg/kg) and manganese (31 mg/kg). Gut-loading diets for silkworms should be supplemented to contain 23 g calcium per kg feed.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10082/abstract]&lt;br /&gt;
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Insects (mealworms, waxworms, crickets, fruitflies) on average have low calcium concentrations (0.11%), but sufficient concentrations of Cu, Fe, Mg, P, and Zn to meet known requirements of domestic birds and mammals. Supermealworms (Zophobas morio) and waxworms contain deficient levels of manganese. Earthworms meet all dietary mineral requirements. [http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract]&lt;br /&gt;
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Intestinal microorganisms in Tilapia nilotica produce at least 11.2 ng of vitamin B12 per g of body weight per day. In 16-weeks without Dietary B12, fish weight increased 8-fold (normal growth), and liver-stored B12 did not decrease.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1982)111%3C485%3AISADNO%3E2.0.CO%3B2#.VCvqCfl_s10] Magnesium absorption from the gastrointestinal tract may be highly efficient in Oreochromis mossambicus.[http://www.ncbi.nlm.nih.gov/pubmed/24194247]&lt;br /&gt;
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==Supplements==&lt;br /&gt;
[[Image:Allspice.jpg|thumb|right| Allspice [http://en.wikipedia.org/wiki/Allspice#mediaviewer/File:AllspiceBowl.JPG © Jonathunder]]]&lt;br /&gt;
[[Image:Babysbreath.jpg|thumb|right| Baby&amp;#039;s breath [http://en.wikipedia.org/wiki/Gypsophila_paniculata#mediaviewer/File:Gypsophila_paniculata.jpg © PiPi]]]&lt;br /&gt;
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* Saponin-rich plants such as Quillaja saponaria (Soap bark tree), yucca and Sapindus saponaria (wingleaf soapberry, western soapberry, jaboncillo) may increase growth, increase the nce male to female ratio in tilapia [http://www.ncbi.nlm.nih.gov/pubmed/22444893] and increase serum cholesterol in males.[http://www.sciencedirect.com/science/article/pii/S1532045602001679] Gypsophila paniculata (baby&amp;#039;s breath) and Quillaja saponaria (both high in saponins) may induce release of LH (Luteinizing hormone)[http://www.ncbi.nlm.nih.gov/pubmed/15792626] Supplementing Tilapia fry with 700 mg/kg extracted Quillaja saponin extract resulted in significantly increased growth, higher number of males, higher LH release and higher serum cholesterol.[http://www.ncbi.nlm.nih.gov/pubmed/12458187] Saponin supplementation diminishes egg production by females.[http://www.ncbi.nlm.nih.gov/pubmed/11423383] Intestinal magnesium uptake in tilapia is stimulated by chloride and inhibited by saponins (which may induce intravesicular magnesium accumulation.[http://www.ncbi.nlm.nih.gov/pubmed/8952951] In tilapia, saponins selectively permeabilize the plasma membrane.[http://www.ncbi.nlm.nih.gov/pubmed/24194248]&lt;br /&gt;
* Allspice Pimenta dioica (10 g/kg fish) acts as a growth promoter to improve feed utilization and weight gain in Mozambique Tilapia fry and acts an antimicrobial agent to enhance disease resistance during first feeding of fry.[http://www.ncbi.nlm.nih.gov/pubmed/25229484] Allspice (aka Jamaica pepper, myrtle pepper, pimenta, pimento, English pepper, newspice) is dried unripe berries of Pimenta dioica.&lt;br /&gt;
* Honey bee pollen (2.5% (w/v)) increases growth performance parameters, feed efficiency ratio and immunological, hematological and biochemical parameters in young Nile tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25086230]&lt;br /&gt;
* A supplemental mixture (at 0.5 to 2% w/w) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle may increase lysozyme, superoxide dismutase and peroxidase activity, decrease malondialdehyde levels and reduce mortality in Aeromonas hydrophila-challenged tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24925761] 20 days administration of 1% Astragalus root (Radix astragalin seu Hedysari) plus Angelica Root (Angelicae Sinensis) at a ratio of 5:1 (w/w) increased body weight of carp relative to controls.[http://www.ncbi.nlm.nih.gov/pubmed/15123322] Another study found no effect on growth by an mixture of Astragalus membranaceus, orange peel, hawthorn, pilose asiabell root indigowoad root, taraxacum and malt.[http://www.ncbi.nlm.nih.gov/pubmed/18378466] Astragalus (aka milkvetch, locoweed) belongs to the legume family Fabaceae. Dietary Astragalus polysaccharides supplementation may improve growth performance and immune parameters of tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24657260] Calycosin is a major isoflavonoid extracted from astragalus. It has shown to have proangiogenic effects.[http://www.ncbi.nlm.nih.gov/pubmed/21909574] Two isomeres extracted from Astragalus membranceus may stimulate proliferation in human fetal lung diploid fibroblast cells.[http://www.ncbi.nlm.nih.gov/pubmed/14659591] &lt;br /&gt;
* Survival in Streptococcus agalactiae challenged tilapia is increased by inclusion of 0.1% Sophora flavescens (root extract) in the diet.[http://www.ncbi.nlm.nih.gov/pubmed/23092731] Sophora flavescens (member of the Fabaceae family) roots contain quinolizidine alkaloids, including matrine, oxymatrine and lupeol. Matrine inhibits cell proliferation [http://www.ncbi.nlm.nih.gov/pubmed/24653570] (including proliferation of cancer cells)[http://www.ncbi.nlm.nih.gov/pubmed/24137393][http://www.ncbi.nlm.nih.gov/pubmed/24137358] Matrine prevents cardiac arrhythmias by inhibiting ouabain-induced calcium overload in guinea pigs.[http://www.ncbi.nlm.nih.gov/pubmed/24680622] Matrine has neurotoxic properties in Zebrafish (Danio rerio) larvae.[http://www.ncbi.nlm.nih.gov/pubmed/24911943]&lt;br /&gt;
&lt;br /&gt;
==Dissolved oxygen==&lt;br /&gt;
Fish take up oxygen through the limited gill surface area. At low dissolved oxygen (DO), more metabolic energy is used by the ventilatory system to maintain O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; uptake, which limits weight gain and growth.[http://www.sciencedirect.com/science/article/pii/0044848694902364] Feed intake and growth at 5.6 ppm (mg/L) DO is significantly higher than at 3.0 ppm, particularly in big fish.[http://www.sciencedirect.com/science/article/pii/S0044848608000021] For fish over 200 grams, feed intake continuous to increase over 3.0 ppm DO (with 5.5 ppm being the incipient DO), but digestibility is higher at suboptimal DO.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2011.02882.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
DO levels in ponds increase during the night due to vertical circulation. Organic materials consume oxygen during decomposition.[http://deepblue.lib.umich.edu/bitstream/handle/2027.42/27066/0000056.pdf?sequence=1] DO levels sharply decline after feeding. Dissolved oxygen levels should be maintained above 5.0 ppm for best growth. At DO levels between 3.0–5.0 ppm feeding should be reduced, and feeding should be stopped at DO levels below 3.0 ppm. Low DO increases the toxicity of ammonia (NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; unionized ammonia &amp;gt; 2.0 mg/L is lethal for tilapia, growth is inhibited below 1.0 mg/L). &lt;br /&gt;
[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf]&lt;br /&gt;
Under recirculated water conditions, mean body weight of Oreochromis aureus was higher at 6.5 ppm DO (mg/L), but food conversion ratio was best at 3.7 ppm DO.[http://www.sciencedirect.com/science/article/pii/0144860995000135]&lt;br /&gt;
Intermediate densities of phytoplankton produce higher dissolved oxygen concentrations. Dissolved oxygen levels may be raised by increasing algal growth, not necessarily by reducing algal biomass.[http://www.sciencedirect.com/science/article/pii/0044848688903572] DO concentrations at dawn is not related to manure input.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract] Low DO increases mortality due to Streptococcus infection.[http://www.sciencedirect.com/science/article/pii/S004484860000346X]&lt;br /&gt;
&lt;br /&gt;
==Salinity==&lt;br /&gt;
[[Image:Saisola_soda_3.jpg|thumb|left| Salsola soda [http://www.invasive.org/browse/detail.cfm?imgnum=5374910 © Joseph M. DiTomaso]]]&lt;br /&gt;
[[Image:Saisola_soda_2.jpg|thumb|right| Salsola soda [http://www.magicgardenseeds.com/SAL02 © MagicGardenSeeds]]]&lt;br /&gt;
[[Image:Saisola_kali_3.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_3.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Saisola_kali_2.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_2.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis niloticus&amp;#039;&amp;#039; may grow better in 7.5 ppt than in 0 ppt salinity [http://www.sciencedirect.com/science/article/pii/S0044848605000888] and may fairly tolerate salinity up to 10 ppt [http://www.sciencedirect.com/science/article/pii/0044848685901024] Tilapia may tolerate 0.5% salinity (5 ppt).[http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf] &lt;br /&gt;
At 8 g / L (8 ppt) salinity adult Oreochromis niloticus may be stocked at 40 fish / m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;. At 15 g/L (15 ppt) salinity total production is more than halfed.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2006.01605.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Hatching success for eggs spawned by yearling females may be similar in freshwater (30.9%), 10 ppt (32.7%) and 15 ppt (36.9%), and considerably higher for eggs spawned at 5 ppt (51.6%). [http://www.sciencedirect.com/science/article/pii/0044848685900882] Energy required by Oreochromis niloticus for osmoregulation is the least in the absence of an osmotic gradient (11.6‰ salinity) [http://www.nrcresearchpress.com/doi/abs/10.1139/f69-277#.VEY_ufnV9JA] In freshwater, heating water to temperatures above 27°C is not justifiable, while at 18 or 36 ppt salinity, heating water to 32°C can maximize growth rates without lowering growth efficiency.[http://www.sciencedirect.com/science/article/pii/004484869390392C] An increase in salinity inhibits growth, compensated by higher temperatures. Juvenile Oreochromis niloticus may be grown similarly in 28°C and freshwater as in 32°C and 8 ppt salinity.[http://www.sciencedirect.com/science/article/pii/S0044848696013609]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis mossambicus&amp;#039;&amp;#039; tend to have lower growth rates than Oreochromis niloticus.&lt;br /&gt;
Oreochromis mossambicus is euryhaline and grows more rapidly in brackish water. (50% seawater)[http://www.ncbi.nlm.nih.gov/pubmed/11248987] Seawater salinity is 35 ppt. Oreochromis mossambicus reared in salt water grows significantly larger than fish reared in fresh water. [http://www.sciencedirect.com/science/article/pii/S1096495903002458] This may be due to salt secretion by highly activated chloride cells in branchial and opercular epithelia [http://www.bioone.org/doi/abs/10.2108/zsj.17.149] and/or a decrease in the resting metabolic rate (which decreased with increasing fish size).[http://www.sciencedirect.com/science/article/pii/0044848695010130] In freshwater, Oreochromis mossambicus prefers 32°C, and higher temperatures with increasing salinity.[http://onlinelibrary.wiley.com/doi/10.1111/j.1752-1688.1986.tb01876.x/abstract] Increasing dietary energy concentration increased mortality in Oreochromis mossambicus. The optimum protein to energy ratio for rapid growth, efficient feed conversion and maximum retention of protein and energy appears to be approximately 23.8 mg of protein per kJ energy.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1995.tb00935.x/abstract] Addition of 17α-methyltestosterone enhances its growth beyond that produced by masculinization alone.[http://www.sciencedirect.com/science/article/pii/0044848693903472] Of the administered 17α-methyltestosterone, less than 1% remains after 100 hours following withdrawal.[http://www.sciencedirect.com/science/article/pii/0044848683900959] Oreochromis mossambicus may cope with either high osmolality (564 mOsm/L), 10 pH or 40°C for several weeks.[http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.1989.tb00440.x/abstract]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis mossambicus&amp;#039;&amp;#039; (♂) &amp;#039;&amp;#039;× Oreochromis niloticus&amp;#039;&amp;#039; (♀) may produce 100% male offspring (&amp;#039;red hybrid tilapia&amp;#039;) [http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0471048267.html](p.363), but spawning frequency may be very low [http://www.sciencedirect.com/science/article/pii/S0044848605000888] and infiltration of parental broodstock by hybrids may be difficult in mass production.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1994.tb00743.x/abstract] (Thai) Red Tilapia has sexual growth dimorphism in which males grow significantly faster than females. Sex reversal may be induced by fluoxymesterone in 4 days old fry.[http://www.ist.cmu.ac.th/researchunit/pcrnc/paper/bio/8.pdf] In 12 ppt salinity, 5 to 12 days old Red Hybrid fry (stocked at 5,660 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;) supplemented with 60 mg 17αethynyltestosterone (ET)/kg diet for at least 7-14 days produces nearly all-male populations (94.3-98.1%).[http://www.tandfonline.com/doi/abs/10.1300/J028v02n01_02#.VKb0-iuG_QM] &lt;br /&gt;
Oreochromis mossambicus (♀) × Oreochromis niloticus (♂) hybrids are relatively salinity tolerant. During 2 months, fingerling growth was not significantly different in 0 ppt, 17 ppt and 32 ppt salinity.[http://onlinelibrary.wiley.com/doi/10.1111/j.1749-7345.2001.tb00930.x/abstract] Median Lethal Salinity (50% survival in 96 h after transfer from freshwater) ranged from 17.2‰ at 30 days post-hatching to 26.7‰ at 60 days post-hatching.[http://www.sciencedirect.com/science/article/pii/0044848685902200] This may be due to Morphological changes in the oesophageal epithelium.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1988.tb05352.x/abstract] The hybrid is superior to Oreochromis mossambicus at all salinities and to Oreochromis niloticus at salinities above 10 ppt.[http://www.sciencedirect.com/science/article/pii/S0044848605000888] This hybrid may be deprived of food during one week in a 8 weeks period without negatively affecting growth.[http://www.sciencedirect.com/science/article/pii/S0044848600003537] Due to starvation, the percentage of polyunsaturated fatty acids (PUFA) increases significantly in these hybrids.[http://www.sciencedirect.com/science/article/pii/S0044848697000355] Growth rate, conversion efficiency and maximum food consumption in Red Hybrid Tilapia may be optimal at 28°C [http://europepmc.org/abstract/MED/11767691] and in 0 to 15 ppt salinity.[http://new.medigraphic.com/cgi-bin/resumenMainI.cgi?IDARTICULO=5732]&lt;br /&gt;
&lt;br /&gt;
==Alkalinity &amp;amp; pH==&lt;br /&gt;
&lt;br /&gt;
Alkalinity is the &amp;quot;buffering&amp;quot; capacity of water (as containing carbonates and bicarbonates) to resist a change in pH (pH fluctuations). In general, culture waters with pH below 7.0 and total alkalinity below 50 mg /L will get benefits by liming.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] Liming is the application of high calcium-carbonate (and/or high magnesium-carbonate) rocks to the water. To increase pH, add sodium carbonate (Na&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;) or calcium carbonate (CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;). Sodium carbonate is more effective due to its higher solubility. Sodium carbonate may be extracted (as Barilla) from the ash of plants growing in sodium-rich soils, such as Halogeton sativus (aka Salsola sativa), Salsola soda and Salsola kali. The harvested plants are dried and subsequently burned. From this ash, the (water soluble) sodium carbonate is extracted with water. This solution is boiled dry, resulting in &amp;quot;barilla&amp;quot;.[http://en.wikipedia.org/wiki/Barilla] Calcium carbonate is the main component in snailshells and eggshells, and present in rocks (particularly limestone, chalk, marble and travertine). To increase alkalinity, one may add sodium bicarbonate, or limestone rocks, so that the water will dissolve large amounts of calcium and magnesium.[http://www.sbz.org.br/revista/artigos/66268.pdf] To create sodium bicarbonate, sodium carbonate may be dissolved in water and treated with carbon dioxide. Solid sodium bicarbonate will precipitate. To increase the alkalinity of 10.000 L water with 10 ppm, add 168 g. of sodium bicarbonate.[http://www.expertpool.biz/ph_alkalinity.htm] Adding sodium carbonate or calcium carbonate decreases the level of free ammonium ions.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
In waters with algae (eg Spirulina), the algae will take up CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; for photosynthesis. As a result, less CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; reacts with H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O to form carbonic acid. This turns HCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt; (bicarbonate) and CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-2&amp;lt;/sup&amp;gt; (carbonate) ions into the&lt;br /&gt;
major forms of inorganic carbon, increasing alkalinity (and DO).[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2000.00493.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; may also be withdrawn (which results in an increas in pH) by adding (bi)carbonates. (Example formula: Na&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; + H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O =&amp;gt; 2NaHCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;)[http://books.google.nl/books?id=Sz4RYEyH5DsC&amp;amp;dq=Pond+aquaculture+water+quality+management&amp;amp;lr=&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] &lt;br /&gt;
&lt;br /&gt;
The appropriate levels for aquaculture are 20-150 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L alkalinity and 7.4-8.2 pH.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
Adding sodium carbonate to water with 20 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity, increasing alkalinity up to 77 meq/L, does not affect &amp;#039;&amp;#039;Oreochromis niloticus&amp;#039;&amp;#039; growth rates. Calcium carbonate is better for tilapia growth, as increasing water hardness as well.[http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/download/8510/8510] Total hardness refers to the content of soluble Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; and Mg&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; in the water. Tilapia need to absorb these ions from the gills for several physiological functions. When total alkalinity is much greater than hardness, water pH may reach 11 in the mid-afternoon due to photosynthesis, because more free CO&amp;lt;sup&amp;gt;2-&amp;lt;/sup&amp;gt;&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; remains in solution.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] Acidifying processes decrease alkalinity, but not hardness of the water.&lt;br /&gt;
&lt;br /&gt;
Fish stocked at higher densities may show better survival rates when total water alkalinity is increased from 30 to 80 or 130 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; per L.[http://onlinelibrary.wiley.com/doi/10.1111/j.1749-7345.2007.00118.x/abstract] Water alkalinity of 35 to 53 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L water may be optimum for larval tilapia growth.[http://www.ablimno.org.br/acta/pdf/acta_limnologica_contents1604E_files/Art4_16(4).pdf] For actual optimum alkalinity levels for Oreochromis niloticus growth, the total hardness/total alkalinity ratio seems to be key and needs to be greater than 1.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] Growth of male sex reversed juvenile Oreochromis niloticus is higher at 146 mg/L calciumcarbonate than at 82 mg/L (the difference is actually in calciumcarbonate). A simultaneous increase in water hardness and alkalinity (due to the application of CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;) induces better growth than only an increase in alkalinity. The best conditions for Oreochromis niloticus growth are 7.4-8.2 pH; total alkalinity &amp;gt; 50 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L; calcium hardness &amp;gt; 140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;/L and free CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; &amp;lt; 7 mg/L, [http://eduem.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6263] though free CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; in water, even when in high concentrations, seems to be harmless to fish when there are suitable concentrations of dissolved oxygen in water.[http://onlinelibrary.wiley.com/doi/10.1111/j.1749-7345.2000.tb00889.x/abstract] To lower a too high pH, one may add CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; (or duckweed) to high-DO water. &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Oreochromis mossambicus&amp;#039;&amp;#039; may thrive in water with 245 mg/L bicarbonates [http://www.tandfonline.com/doi/abs/10.1080/07438140709354036#.VKriGyuG_QM] or 289 mg/L total alkalinity and 8.8 to 9.4 pH [http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1981)110%3C239%3ADAAOPD%3E2.0.CO%3B2#.VKwPHyuG_QM][http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1977.tb05129.x/abstract][http://www.sciencedirect.com/science/article/pii/0883292796000339] or 7.9 to 8.6 pH (dominant fork length is 24 cm)[http://aciar.gov.au/files/node/329/pr98_pdf_69082.pdf#page=66], and may cope with 10 pH for several weeks.[http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.1989.tb00440.x/abstract]&lt;br /&gt;
&lt;br /&gt;
==Water flow==&lt;br /&gt;
A flow rate of 0.5 L/min per kg biomass appears to be desirable for intensive culture of tilapia.[http://www.sciencedirect.com/science/article/pii/004484869190073G]&lt;br /&gt;
&lt;br /&gt;
==Stocking density==&lt;br /&gt;
Tilapia needs to be stocked by age group to prevent cannabalism. The risk of cannabalism increases with increasing size differences.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA465&amp;amp;dq=algae+tilapia&amp;amp;ots=BVjAbOF-zV&amp;amp;sig=aEIMIY5ekRp5adhtpwsOIqPQTMQ#v=onepage&amp;amp;q=algae%20tilapia&amp;amp;f=false] Given reasonable assumptions about production costs, the optimal final density of Oreochromis niloticus in a tank-based flow-through system is 73.7 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.ncbi.nlm.nih.gov/pubmed/23915501]&lt;br /&gt;
Combined net yield of both caged and open-pond tilapia was highest in the treatment with 50 large fish (av. 141 g.) per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.sciencedirect.com/science/article/pii/S0044848696013774]&lt;br /&gt;
Broodstock may be best stocked at 4 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1999.00311.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
Red tilapia stocked in a pulsed-flow aquaculture system at densities of 10 and 20 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; had significantly higher individual weights than fish stocked at 30, 50, and 70 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;, due to limiting water quality; ammonia concentrations may be high (4 mg/L), and dissolved oxygen low (2 mg/L).[http://www.tandfonline.com/doi/abs/10.1300/J028v05n01_08#.VEY9SvnV9JA] Oreochromis niloticus fry may grow optimally when stocked at 5 fry / L and fed at 30% bodyweight / day.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2002.00700.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Oreochromis niloticus fingerlings may be stocked at a density of 42.6/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; with a production of 18–20 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; in 210 days at a water flow rate of 1 L/min/kg fish biomass.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1989.tb00440.x/abstract] With dissolved oxygen levels below 2 ppm (or even below 1 ppm) most of the time, stocking 3 fish per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; was more profitable due to increased mortality and lower growth rates at higher stocking densities (6 and 9 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;), despite similar water qualities.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/487.pdf]&lt;br /&gt;
&lt;br /&gt;
==Growth==&lt;br /&gt;
Tilapia nilotica fingerlings grow optimally on diets containing 30-40% protein, 12-15% lipids, and 30-40% digestible carbohydrate.[http://ir.kagoshima-u.ac.jp/bitstream/10232/15661/1/AN00181784_v6-1_p56-71.pdf]&lt;br /&gt;
Where extra protein (from 25% to 30%) did not increase growth in young tilapia (av. 18 cm long, 62 g. weight), increasing feeding levels to 2% of bodyweight / day did.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
juvenile Oreochromis niloticus grow better at 26 and 30°C, than at 22 and 34°C.[http://www.sciencedirect.com/science/article/pii/S0306456507001118] Feed efficiency and protein efficiency ratio are better and juvenile fish grow better at 28°C than at 22°C and 34°C. Liver gluconeogenesis and lipogenesis are increased by low temperature (22°C).[http://www.ncbi.nlm.nih.gov/pubmed/24556257] Growth and feed utilization efficiency is similar in genetically improved farmed tilapia (GIFT), genetically male Nile tilapia (GMNT) and conventional Nile tilapia (CNT).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2007.01679.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Large Oreochromis niloticus (av. 288 g.) grow less in recirculating aquaculture systems compared to flow-through systems. The opposite is true for small fish (av. 81 g.)[http://www.sciencedirect.com/science/article/pii/S0044848609008254]&lt;br /&gt;
&lt;br /&gt;
The use of tuna by-product meal as a total replacement for fish meal into tilapia diets increases oxidative stress.[http://www.ncbi.nlm.nih.gov/pubmed/25139749]&lt;br /&gt;
&lt;br /&gt;
Higher energy conservation is achieved when fish are exposed to longer rather than shorter photoperiod cycles.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00450.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
If reproduction can be delayed in the females, average growth rates comparable to those of an all-male population may be achieved.&lt;br /&gt;
[http://www.sciencedirect.com/science/article/pii/004484869390034V]&lt;br /&gt;
&lt;br /&gt;
==Strains==&lt;br /&gt;
&lt;br /&gt;
Compared to Oreochromis niloticus strains from Egypt and Senegal (bred from wild collected stocks), and ‘Israel’, ‘Singapore’, ‘Taiwan’ and ‘Thailand’ (strains maintained for aquaculture purposes), a strain bred in Ghana from wild collected stocks showed a significantly lower body weight at 210 days.&lt;br /&gt;
[http://www.sciencedirect.com/science/article/pii/004484869390034V]&lt;br /&gt;
&lt;br /&gt;
Triploidy may be induced in Oreochromis niloticus by heat shock. No differences in growth between triploids and diploid controls may be observed at age of maturation, but triploids are significant heavier (P &amp;lt; 0.01) than control fish at the end of the experiment. Triploid males exceeded body weights of respective controls on average by 66 ± 17%, whereas triploid females displayed an even higher increase in body weight compared to diploid ones (95 ± 27%).[http://www.sciencedirect.com/science/article/pii/0044848695011048]&lt;br /&gt;
&lt;br /&gt;
==Masculinization==&lt;br /&gt;
Estrogen synthesis is crucial for ovarian differentiation, and transcription of the aromatase gene can be proposed as a key step in that process in fish. Treatments with an aromatase inhibitor (ATD, 1,4,6- androstatriene-3-17-dione) result in 75.3% masculinization of an all-female (XX) population in tilapia (dosage 150 mg/kg of food). The effectiveness of the aromatase inhibition by ATD is demonstrated by the marked decrease of the gonadal aromatase activity in treated animals versus control.[http://www.ncbi.nlm.nih.gov/pubmed/10471475]&lt;br /&gt;
&lt;br /&gt;
Aromatase activity as a key factor in sexual differentiation in Oreochromis niloticus.[http://www.ncbi.nlm.nih.gov/pubmed/11424212] The most sensitive time to aromatase inhibitors lies in the first week (between 7 and 14 days post hatch). Treatment with the aromatase inhibitor Fadrozole (nonsteroidal) showed a dose-dependent increase in the percentage of males from 0 to 200 mg per kg. At higher doses (200 to 500 mg / kg), the percentage of males remained more or less constant (92.5-96.0%).[http://www.ncbi.nlm.nih.gov/pubmed/10861549]&lt;br /&gt;
&lt;br /&gt;
The masculinizing actions of 17alpha-methyltestosterone (MT) are most potent at up to day 20 of age.[http://www.ncbi.nlm.nih.gov/pubmed/11694764] Treating female tilapia Oreochromis niloticus with methyltestosterone (at a dose of 50 mcg/g diet) resulted in 100% masculinization.[http://www.ncbi.nlm.nih.gov/pubmed/16115634] Fry may be fed a commercial diet (SRT-95) with 30 ppm α-methyltestosterone for 21 days to yield 99% males.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA183&amp;amp;dq=stocking+density+oreochromis+niloticus+flow-through&amp;amp;ots=BVjC9QG1uV&amp;amp;sig=oZEoAvtMefOYOjPjqXQ-tbd_z_w#v=onepage&amp;amp;q&amp;amp;f=false] Numerous papers have reported that adding 17α-methyltestosterone at 60 mg/kg to the diet for several weeks induces mono-sex male populations. During growing-out season, muscle testosterone concentration in monosex tilapia was sharply decreased to 2.625 ± 0.303 ng/g at the harvesting date. [http://www.ejabf.eg.net/pdf/Vol-17-n-2/Issue%206.pdf]&lt;br /&gt;
&lt;br /&gt;
Treatment with tamoxifen and letrozole (200mg/kg feed) to fingerlings of O. niloticus for 60 days brought about 98.5% masculinization.&lt;br /&gt;
Treatment with 17α methyltestosterone (35 mg/kg feed) to fingerlings of O. mossambicus after 8 days post hatch for 60 days obtained 100% sex reversed males with excellent growth.[http://www.ncbi.nlm.nih.gov/pubmed/23063432]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. The critical period for elevated-temperature-induced masculinization lies between days 10 and 15 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] Higher temperatures (during 5 days) before they are 5 days old induces deformities. Masculinization is induced at elevated temperatures (28 to 32°C) during 5 days after 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577] The advantage of producing faster growing males of Nile tilapia at high temperature would hardly compensate the loss of production incurred during the masculinising treatment.[http://www.sciencedirect.com/science/article/pii/S004484860000452X] High-temperature (almost 36.90 °C) treatments yield a significantly higher proportion of males (64.20–80%) with lower survival rates (60–81%). The sex ratio of progenies reared at temperature below 36°C never deviated significantly from the balanced sex ratio.[http://www.sciencedirect.com/science/article/pii/S0306456507001118]&lt;br /&gt;
&lt;br /&gt;
==Feminization==&lt;br /&gt;
The critical period for low-temperature-induced feminization lies between days 5 and 10 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] The period of maximal feminizing action of 17beta-estradiol (E(2)) upon sex ratio is before 10 days posthatching in tilapia (Oreochromis mossambicus).[http://www.ncbi.nlm.nih.gov/pubmed/11694764]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. Higher temperatures (during 5 days) before they are 5 days old induces deformities. Gonadal feminization is induced at lower temperatures (20°C) for 5 days before 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577]&lt;br /&gt;
&lt;br /&gt;
==Parasites==&lt;br /&gt;
Tilapia may be infected with: &lt;br /&gt;
&lt;br /&gt;
* Nematodes / roundworms, such as Contracaecum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Contracaecum multipapillatum [http://www.ncbi.nlm.nih.gov/pubmed/24573461]), Paracamallanus cyathopharynx, Procamallanus laevionchus [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Rhabdochona esseniae [http://www.ncbi.nlm.nih.gov/pubmed/7596571], Rhabdochona paski [http://www.ncbi.nlm.nih.gov/pubmed/23595492], Gnathostoma spinigerum, Gnathostoma doloresi and Gnathostoma hispidum.[http://www.ajtmh.org/content/58/3/316.long] Human gnathostomiasis infection is related to ingestion of &amp;quot;ceviche&amp;quot;.[http://www.ncbi.nlm.nih.gov/pubmed/2588073]&lt;br /&gt;
* Cestodes (aka tapeworms), such as Amirthalingamia sp. (eg Amirthalingamia macracantha; higher prevalence and intensity in wet season [http://www.ncbi.nlm.nih.gov/pubmed/21972071]), and Cyclustera sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Cyclustera magna [http://www.ncbi.nlm.nih.gov/pubmed/15357392]) &lt;br /&gt;
* Trematodes / flukes (encysted metacercariae)[http://www.ncbi.nlm.nih.gov/pubmed/19795754], such as Clonorchis sinensis (human liver trematode)[http://www.ncbi.nlm.nih.gov/pubmed/22471793], Tylodelphys sp.[http://www.ncbi.nlm.nih.gov/pubmed/24407916], Stictodora tridactyla (in brackish-water fish)[http://www.ncbi.nlm.nih.gov/pubmed/2332641], Ribeiroia marini [http://www.ncbi.nlm.nih.gov/pubmed/24996], Clinostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] ((eg Clinostomum Complanatum, Clinostomum tilapiae, Euclinostomum hetereostomum [http://www.ncbi.nlm.nih.gov/pubmed/23113215]), Diplostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Neascus sp, Acanthostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Bolbophorus sp.[http://www.ncbi.nlm.nih.gov/pubmed/21972071], Haplorchis yokogawai [http://www.ncbi.nlm.nih.gov/pubmed/21073146], Haplorchis pumilio, Haplorchis taichui, Centrocestus formosanus, Stellantchas musfalcatus, Echinochasmus japonicus [http://www.ncbi.nlm.nih.gov/pubmed/18564743], Heterophyes heterophyes, Heterophyes aequalis, Pygidiopsis genata, Stictodora sp., Phagicola sp.[http://www.ncbi.nlm.nih.gov/pubmed/18339484] (eg Phagicola ascolonga[http://www.ncbi.nlm.nih.gov/pubmed/21073146]) and Prohemostomumn vivax.[http://www.ncbi.nlm.nih.gov/pubmed/18383799] These trematodes loose their viability after 48 hours at -5°C.[http://www.ncbi.nlm.nih.gov/pubmed/19795748] The conditions of gas supersaturation (gas pressure &amp;gt; 111.2% saturation; N supersaturation, O2 undersaturation) may result in heavy monogenetic trematode infections in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/9234554] In Thailand, fish-borne zoonotic trematode (FZT) metacercarial infections were found in Nile tilapia from cage (2.5%) and pond aquaculture systems (10%) and in wild caught fish.(53%) [http://www.ncbi.nlm.nih.gov/pubmed/24029716] In China, tilapia from nursery and grow-out ponds were sampled from monoculture, polyculture and integrated aquaculture systems, revealing a 1.5% prevalence of FZT infections (Heterophyidae and Echinostomatidae); lower than in wild caught fish. Integrated systems using animal manure and latrine wastes as fertilizer did not show a higher prevalence.[http://www.ncbi.nlm.nih.gov/pubmed/24018184] In Vietnam, the overall FZT prevalence in tilapia from wastewater ponds was 2.0%, but much higher in tilapia from farm ponds. [http://www.ncbi.nlm.nih.gov/pubmed/22471793]&lt;br /&gt;
* Monogenea (very small flatworms), such as Dactylogyrus minutus [http://www.ncbi.nlm.nih.gov/pubmed/24407916], Neobenedenia melleni [http://www.ncbi.nlm.nih.gov/pubmed/7707197][http://www.ncbi.nlm.nih.gov/pubmed/1597779], Enterogyrus cichlidarum (associated with overcrowding [http://www.ncbi.nlm.nih.gov/pubmed/7472888] and infection intensity increases with (tilapia) host size[http://www.ncbi.nlm.nih.gov/pubmed/2242958]), Gyrodactylus malalai (ectoparasite)[http://www.ncbi.nlm.nih.gov/pubmed/22807048], Gyrodactylus ergensi [http://www.ncbi.nlm.nih.gov/pubmed/19838735], Scutogyrus longicornis [http://www.ncbi.nlm.nih.gov/pubmed/25054495] and of the genus Cichlidogyrus [http://www.ncbi.nlm.nih.gov/pubmed/22436463], such as Cichlidogyrus tilapiae, Cichlidogyrus sclerosus [http://www.ncbi.nlm.nih.gov/pubmed/21791155], Cichlidogyrus dossoui [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Cichlidogyrus halli [[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Cichlidogyrus thurstonae [http://www.ncbi.nlm.nih.gov/pubmed/14707506], Cichlidogyrus berradae, Cichlidogyrus revesati, Cichlidogyrus legendrei and Cichlidogyrus lemoallei.[http://www.ncbi.nlm.nih.gov/pubmed/14535345] The highest parasite number was recorded in reservoir-dwelling, and lowest in stream-dwelling tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21791155] Gyrodactylus niloticus infection may particularly become lethal (in tilapia) when followed by exposure to the bacterial pathogen Streptococcus iniae.[http://www.ncbi.nlm.nih.gov/pubmed/17394525] Low stocking density and low water temperature may result in lower monogenea parasitism rate.[http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Acanthocephala (thorny-headed worms), such as Acanthogyrus (Acanthosentis) tilapiae.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] and Polyacanthorhynchus kenyensis [http://www.ncbi.nlm.nih.gov/pubmed/12015820] In Kenya, infection rates in wild Tilapia zillii ranged from 4.1 to 77.7%.[http://www.ncbi.nlm.nih.gov/pubmed/9332980] &lt;br /&gt;
* Ciliatea (&amp;#039;small flagella&amp;#039;), such as Epistylis sp.[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Paratrichodina africana [http://www.ncbi.nlm.nih.gov/pubmed/23731856], Trichodina compacta [http://www.ncbi.nlm.nih.gov/pubmed/21755153], Trichodina magna [[http://www.ncbi.nlm.nih.gov/pubmed/25054495] and Ichthyophthirius multifiliis.[http://www.ncbi.nlm.nih.gov/pubmed/22311586] Ichthyophthirius multifiliis load increases susceptibility and mortality of tilapia to Streptococcus iniae (Gram-positive bacteria) infection.[http://www.ncbi.nlm.nih.gov/pubmed/19750806] &lt;br /&gt;
* Dinophyceae (Dinoflagellata; &amp;quot;red tide&amp;quot;), such as Piscinoodinium pillulare.[http://www.ncbi.nlm.nih.gov/pubmed/25054495] (a dominant parasite in Brasilian tilapia ponds)[http://www.ncbi.nlm.nih.gov/pubmed/21755153] &lt;br /&gt;
* Myxosporea, such as Henneguya suprabranchiae [http://www.ncbi.nlm.nih.gov/pubmed/18516619], Myxobolus heterosporus [http://www.ncbi.nlm.nih.gov/pubmed/15819436] (induces total destruction of the intestine structure [http://www.ncbi.nlm.nih.gov/pubmed/12911060]), Myxobolus nounensis [http://www.ncbi.nlm.nih.gov/pubmed/11031757], Myxobolus dossoui (host size effect), Myxobolus zillii (seasonal pattern) [http://www.ncbi.nlm.nih.gov/pubmed/11383569], Myxobolus microcapsularis [http://www.int-res.com/articles/dao/44/d044p217.pdf], Myxobolus agolus, Myxobolus brachysporus, Myxobolus clarii, Myxobolus cichlidarum, Myxobolus tilapiae and Myxobolus camerounensis.[http://www.ncbi.nlm.nih.gov/pubmed/19069850]&lt;br /&gt;
* Conoidasida (unicellular), such as Goussia cichlidarum.[http://www.ncbi.nlm.nih.gov/pubmed/17885764]&lt;br /&gt;
* Phyllopharyngea (single-cell parasites), such as Chilodonella hexasticha.[http://www.ncbi.nlm.nih.gov/pubmed/22902259]&lt;br /&gt;
* Trhypochthoniellus longisetus longisetus, a mite.[http://www.ncbi.nlm.nih.gov/pubmed/21553570]&lt;br /&gt;
* Lamproglena sp. (Lernaeidae; small crustaceans) [http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Pentasomida (tongue worms; tiny crustaceans), such as Leiperia cincinnalis [http://www.ncbi.nlm.nih.gov/pubmed/9809320], Subtriquetra wedli [http://www.ncbi.nlm.nih.gov/pubmed/10192834] and Subtriquetra riley.[http://www.ncbi.nlm.nih.gov/pubmed/9809320]&lt;br /&gt;
* Anodontites trapesialis (the larval stage of this mussel).[http://www.ncbi.nlm.nih.gov/pubmed/12048579]&lt;br /&gt;
&lt;br /&gt;
No parasite vaccines exist.[http://www.ncbi.nlm.nih.gov/pubmed/15757476] Tilapia are relatively resistant to parasitic infections.[http://www.ncbi.nlm.nih.gov/pubmed/18537032] The prevalence of heterophyid encysted metacercariae infection decreases as fish size increases.[http://www.ncbi.nlm.nih.gov/pubmed/20644958] When dissolved oxygen, temperature, pH, nitrite and ammonia are within normal rate parasites may cause little harm to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19500461] Parasitic infection by Acanthogyrus tilapiae provokes an aggressive host response.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] Tilapia may produce an induced humoral immune response against Cichlidogyrus sp.[http://www.ncbi.nlm.nih.gov/pubmed/18564741] Oreochromis mossambicus initiated a strong immune protection by direct exposure with even a small number of parasites.[http://www.ncbi.nlm.nih.gov/pubmed/21739314] Gyrodactylus numbers fluctuate independently of temperature. In anticipation of immune defenses reaching the fish surface through mucus, Gyrodactylids (Monogenea) worms progressively move away from fins with high mucus cell density to those with low density.[http://www.ncbi.nlm.nih.gov/pubmed/21840127] Tilapia may also develop resistance to Neobenedenia sp. (Monogenea) infection.[http://www.ncbi.nlm.nih.gov/pubmed/21381523]&lt;br /&gt;
&lt;br /&gt;
Freshwater mollusks in tilapia ponds, such as Melania tuberculata, Melanoides turricula, Pomacea flagellata, Haitia cubensis and Anodontiles luteola, may host various parasites that are harmful to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21250483] The Biomphalaria sp. snails are often found in tilapia ponds (fresh water reservoirs are their natural habitat[http://www.ncbi.nlm.nih.gov/pubmed/11100444]) and are intermediates for Schistosoma mansoni.[http://www.scielo.br/scielo.php?script=sci_arttext&amp;amp;pid=S0074-02762001000900008&amp;amp;lng=en&amp;amp;nrm=iso&amp;amp;tlng=en] Biomphalaria cf. havanensis is an intermediate host for Diplostomum ompactum, causing higher levels of infections in cultured tilapia than wild tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19452167] The snail Bulinus truncatus is an intermediate host for Clinostomum tilapiae, Euclinostomum heterostomum, Bolbophorus levantinus and Neascus-type metacercariae. The snail Melanoides tuberculata is an intermediate host for Centrocestus sp. and Haplorchis sp. The snail Melanopsis costata is an intermediate host for Pygidiopsis genata, Phagicola longa.[http://www.ncbi.nlm.nih.gov/pubmed/12911062] The snail Thiara granifera is commonly infected with Haplorchis pumilio.[http://www.ncbi.nlm.nih.gov/pubmed/12015833] Dogs, cats and pigs may also be intermediate hosts for metacercariae.[http://www.ncbi.nlm.nih.gov/pubmed/18564743]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Talk:Tilapia&amp;diff=4528</id>
		<title>Talk:Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Talk:Tilapia&amp;diff=4528"/>
		<updated>2015-01-05T19:44:59Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Editing Style Hours &amp;amp; Notation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Editing Style&lt;br /&gt;
&lt;br /&gt;
A style should always be selected to serve your main audience (humans, computers, robots...).&lt;br /&gt;
&lt;br /&gt;
Which number style would be best for the Tilapia page?&lt;br /&gt;
&lt;br /&gt;
a) 10.000 L (For readers familiar with European notation.)&lt;br /&gt;
&lt;br /&gt;
b) 10,000 L (For readers familiar with American notation.)&lt;br /&gt;
&lt;br /&gt;
c) 10000 L (For electronic devices that cannot read commas and decimals.)&lt;br /&gt;
&lt;br /&gt;
d) 10.000 L, 10,000 L, and 10000 L (are all acceptable formats, as we intend to keep the original source&amp;#039;s format.)&lt;br /&gt;
&lt;br /&gt;
If your audience prefers to read 10.000 L, then that should be kept.&lt;br /&gt;
&lt;br /&gt;
If they can interpret the number as 10000 L and not 10 L.&lt;br /&gt;
&lt;br /&gt;
 &lt;br /&gt;
Hours: Which style?&lt;br /&gt;
&lt;br /&gt;
a) 12.00&lt;br /&gt;
&lt;br /&gt;
b) 12:00&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Talk:Tilapia&amp;diff=4523</id>
		<title>Talk:Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Talk:Tilapia&amp;diff=4523"/>
		<updated>2015-01-04T19:58:30Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Editing Style Talk ( Numbers and Notations )&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Editing Style&lt;br /&gt;
&lt;br /&gt;
A style should always be selected to serve your main audience (humans, computers, robots...).&lt;br /&gt;
&lt;br /&gt;
Which number style would be best for the Tilapia page?&lt;br /&gt;
&lt;br /&gt;
a) 10.000 L (For readers familiar with European notation.)&lt;br /&gt;
&lt;br /&gt;
b) 10,000 L (For readers familiar with American notation.)&lt;br /&gt;
&lt;br /&gt;
c) 10000 L (For electronic devices that cannot read commas and decimals.)&lt;br /&gt;
&lt;br /&gt;
d) 10.000 L, 10,000 L, and 10000 L (are all acceptable formats, as we intend to keep the original source&amp;#039;s format.)&lt;br /&gt;
&lt;br /&gt;
If your audience prefers to read 10.000 L, then that should be kept.&lt;br /&gt;
&lt;br /&gt;
If they can interpret the number as 10000 L and not 10 L.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4486</id>
		<title>Composting toilet</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4486"/>
		<updated>2014-12-31T19:42:17Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Notation,&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[Image:Urine_diversion.jpg|thumb|left| urine diversion ]]&lt;br /&gt;
[[Image:Composting_toilet.jpg|thumb|right| [http://en.wikipedia.org/wiki/Composting_toilet#mediaviewer/File:Clivus_Multrum_Composting_toilet_with_urine_diversion-dehydration.svg Clivus Multrum] adapted for solar ventilation and urine re-use&lt;br /&gt;
- 1. Humus compartment 2. Ventilation inflow 3. WC 4. Urinal 5. Urine collection 6. Manual paddles 7. Solar heating of ventilation outflow ]]&lt;br /&gt;
[[Image:Urine_diversion_2.jpg|thumb|left| urine diversion - squatting [http://en.wikipedia.org/wiki/Urine-diverting_dry_toilet#mediaviewer/File:Inside_of_the_UDDT_(5332645379).jpg]]]&lt;br /&gt;
&lt;br /&gt;
It is estimated that the excreta from 80 persons could fertilize a hectare. A single adult eats 250 kg of cereals per year, which may be grown on less than 250 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;, fertilised by ~50% of the urine of that person, mixed with the waste water used by that person.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf]&lt;br /&gt;
&lt;br /&gt;
Composting toilets aerobically decompose waste material, by aerobic microorganisms in a highly aerated, warm (32°C - 49°C) and moist (humidity &amp;gt;80%) environment, with ample carbon (sawdust as the sole agent will lead to clogging[http://www.lismore.nsw.gov.au/infocouncil/Open/2013/OC_13082013_ATT_EXCLUDED_WEB.HTM]). During composting, mineralization, and humification occur simultaneously and are the main processes leading to degradation of the fresh organic matter. Organic matter humification in composting occurs when lignin and its degradation products are degraded by the microorganisms.[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] Optimally, the volume of the resulting compost may be about 10% of input.[http://en.wikipedia.org/wiki/Composting_toilet] &lt;br /&gt;
&lt;br /&gt;
Continuous composting toilets are single container toilets that receive excrement which decomposes as it moves slowly through the container and is removed as compost from the end-product chamber. The container is permanently fitted under the toilet seat, and never has to be fully emptied as the compost can be gradually removed when it reaches the end-product chamber. [http://www.yourhome.gov.au/water/waterless-toilets] To make the fresh material move down the slope, the slope needs to be slippery, eg bathroom tiles. Natural zeolite may be used as medium for the sorption of ammonia from wastewater and subsequently as nitrogen releaser in cultures of Spirulina platensis.[http://www.sciencedirect.com/science/article/pii/S0960852413019627]&lt;br /&gt;
&lt;br /&gt;
In a non-urine-diverting system, 90% of what goes into a composting toilet is water. Compost piles need to be damp to work well, but most composting toilets suffer from too much water. (ideally 45 to 70% moisture content [http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets])&lt;br /&gt;
Ammonia may evaporate as nitrogen in a gas form, which is vented out along with carbon dioxide and water vapor.[http://www.google.com/patents/US4096592]&lt;br /&gt;
Evaporation is the primary way a composting toilet gets rid of excess water. Warmth and air flow through the unit assist the evaporation process. Every composting toilet has a vertical vent pipe to carry off moisture. Air flows across the drying trays, around and through the pile, then up the vent to the outside of the building.&lt;br /&gt;
In a cold environment, the low-grade heat produced by composting is supposed to provide sufficient updraft to carry vapor up the vent.[http://buttecreekwatershed.org/BMP/test2/Book6last.htm] In tropical conditions, passive solar heating may create an air draft.&lt;br /&gt;
&lt;br /&gt;
If operation conditions for thermophilic composting are adequate (moisture content 50 to 60%, carbon to nitrogen ratio 30 to 35 and mixing with bulking material), the temperature will rise to between 50 and 65 °C (WHO 2006). Such temperatures will effectively inactivate pathogens (WHO 2006).[http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets] If moisture content is too high, anaerobic organisms will thrive, creating undesirable odors from anaerobic digestion.&lt;br /&gt;
&lt;br /&gt;
==Carbon / Nitrogen ratio==&lt;br /&gt;
Dry material, which contains carbon (such as dry leaves or grasses) increases composting properties. It regulates moisture and the carbon to nitrogen ratio (C/N) and enhances the composting process. The optimal carbon to nitrogen ratio for thermophilic composting is 20 to 35 (WHO 2006). The C/N ratio of humanure (135 to 270 g / person / day) is about 5 to 10 (5%-7% nitrogen) The C/N ratio of grass clippings may be 12-19 and they may contain 2.4% nitrogen. The C/N ratio of leaves may be 48 and they may contain 0.9% nitrogen.[http://www.weblife.org/humanure/chapter3_7.html] Thus 200 g humanure plus 225 g leaves may render a combined C/N ratio of 30.&lt;br /&gt;
The C/N ratio of Panicum texanum is 32.9:1.[http://www.researchgate.net/publication/24186263_Nematode_Population_Fluctuations_during_Decomposition_of_Specific_Organic_Amendments] Thus 200 g humanure plus 1379 to 1724 g. [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Panicum Panicum texanum] may render a combined C/N ratio of 30.&lt;br /&gt;
&lt;br /&gt;
Crude protein in the leaves of Giant cane ([http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Arundinaria_gigantea Arundinaria gigantea]) is relatively low in young leaves.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=8259377&amp;amp;fileId=S1742170510000566] The C/N ratio in Giant cane leaves may be 19.4 (2.4% nitrogen and 45.8% carbon).[http://www.ars.usda.gov/SP2UserFiles/ad_hoc/19320000PosterGallery/AFGC2004_Bamboo_Final.pdf] Bamboo shoots may contain 0.6% nitrogen. Overall bamboo litter (culm, branches, leaves) may contain 1.4% nitrogen.[http://www.sciencedirect.com/science/article/pii/S0378112704006693] The carbon concentration in decomposing bamboo culms may be 50%, irrespective of decomposition level. Mean nitrogen concentration may be 1%.(going up from ~0.3% as decomposition advances) [https://www.deepdyve.com/lp/springer-journals/characteristics-of-culm-structure-and-carbon-and-nitrogen-pP0nfb7X0z/7] Bamboo biochar and wood biochar have the same C/N ratio[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] The C/N ratio of softwood may be 640, of hardwood may be 560 and that of sawdust 500, which goes down as it rots (down to 200).[http://www.weblife.org/humanure/chapter3_7.html] That is because the level of carbon (and Na, K) goes down faster than the level of nitrogen (and P).[http://link.springer.com/article/10.1007/BF00346060#page-1] Nitrogen is usually retained until 50% of the carbon remains.[http://link.springer.com/article/10.1007/s10021-004-0026-x#page-1] For the supply of carbon, one may process wood into sawdust, or one may let the chopped wood decompose naturally (with a lower C/N ratio). &lt;br /&gt;
&lt;br /&gt;
For a resulting combined C/N ratio of 30:&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 21 g                       bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 11 g                       bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 63 g  partially decomposed bamboo wood    (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 24 g  partially decomposed bamboo culms   (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 252 g partially decomposed bamboo culms   (if C/N=50) &lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 200 g partially decomposed bamboo culms   (if C/N=50)&lt;br /&gt;
&lt;br /&gt;
Fruit wastes may have a C/N ratio of 20-50.[http://www.norganics.com/applications/cnratio.pdf] Mango waste (without stone) may have a C/N ratio of 50.[http://www.researchgate.net/profile/DrLakshmi_priya_Thyagarajan/publication/225038970_Bioconversion_of_Mango_Waste_Blended_with_Poultry_Waste_and_Cow_Dung_into_Useful_Manure_by_Aerobic_Composting/links/0fcfd4fbfa557c43e1000000]&lt;br /&gt;
&lt;br /&gt;
==Urine==&lt;br /&gt;
[[Image:Urine-nutrients.jpg|thumb|left| Urine composition - a [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_3.jpg|thumb|right| Urine composition - c [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_2.jpg|thumb|left| Urine composition - b [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
Excessive ammonia from urine inhibits the microbial processes in the chamber. With urine diversion, less bulking agent is needed and the C/N ratio is naturally enhanced.&lt;br /&gt;
The C/N ratio of urine (1-2 L / person / day) is about 0.8 and its nitrogen content is ~15-18%.[http://www.weblife.org/humanure/chapter3_7.html]&lt;br /&gt;
Urine can contain up to 90 percent of the nitrogen, up to 50 percent of the phosphorus, and up to 70 percent of the potassium present in human excreta.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf] It may contain 9.3 g/L urea, 1.9 g/L chloride, 1.2 g/L sodium, 0.8 g/L potassium, 0.5 g/L phosphorus and 0.2 g/L ammonia.[http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf] &lt;br /&gt;
In healthy individuals it is usually pathogen free, although undiluted it may contain inorganic salts and organic compounds at levels toxic to plants. Ureum decomposes into ammonia, which smells.&lt;br /&gt;
&lt;br /&gt;
When applying urine to pastures, 14 to 28% of the nitrogen may evaporate in 2 weeks. 50% of that will be lost within the first 24 hours.[http://www.publish.csiro.au/?paper=AR9820097] The level of nitrogen volatilized as ammonia increases with increasing temperature.[http://link.springer.com/article/10.1007/BF00335848] Urine can be used as a source of complementary nitrogen in high carbon compost. When diluted with water at a 1:8 ratio, it can be used as a fertilizer [http://www.liquidgoldbook.com/], comparable to commercial fertilizers.[http://modernfarmer.com/2014/01/human-pee-proven-fertilizer-future/] &lt;br /&gt;
&lt;br /&gt;
Though carbon is its principal required nutrient, [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia#Spirulina Spirulina platensis] may be grown on human urine [http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] &lt;br /&gt;
At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g/L.[http://link.springer.com/article/10.1007%2FBF02179776#page-1] Since human urine typically contains about 9.3 gram urea per L [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf], 1 L of urine may be added to 5.2 L of clean water (or 161 ml of urine per L clean water).&lt;br /&gt;
Best cellular growth may occur at 500 mg/L of urea.[http://www.sciencedirect.com/science/article/pii/S0960852403002359] (53 ml urine per L clean water) Growth may be optimal when the urea feeding rate is slowly increased [http://www.sciencedirect.com/science/article/pii/S0044848604005952] and at 29°C. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] Best results are obtained by continuous urea addition in exponentially increasing amounts, at 30°C.[http://www.sciencedirect.com/science/article/pii/S0961953402000545] Intermittent addition of urea yields results similar to those obtained by the continuous feeding.[http://www.sciencedirect.com/science/article/pii/S0144860904000263] Spirulina platensis may use several nitrogen sources; most effectively sodium nitrate, ammonium nitrate and urea.[http://link.springer.com/article/10.1023/A:1011925022941#page-1] Spirulina platensis grows better on urea than on potassium nitrate.[http://link.springer.com/article/10.1385/ABAB:112:3:143#page-1] Nitrate may yield a higher dry weight.[http://www.sciencedirect.com/science/article/pii/S0167299104803203] In combination with bicarbonates, urea may be more effective than ammonium and nitrate.[http://docsdrive.com/pdfs/academicjournals/ajpp/0000/47496-47496.pdf] Spirulina, when grown with urea as the nitrogen source, is characterized&lt;br /&gt;
by a higher methionine and arginine content.[http://link.springer.com/article/10.1007/BF00444067#page-1] Urea is the best source for increasing γ-linolenic acid contents in Spirulina [http://link.springer.com/article/10.1007/BF00218450#page-1] and production is elevated in the dark.[http://link.springer.com/article/10.1007/BF02920244#page-1] Though Spirulina platensis may tolerate high levels of urea better [http://link.springer.com/article/10.1007/s10295-005-0025-8#page-1], it is ammonium resistant at high pH.[http://pcp.oxfordjournals.org/content/32/7/953.short] The uptake of ammonia in Spirulina platensis (supporting optimal growth) is pH dependent with an optimum at pH 9.3, proceeding at the same rates in the light and in the dark.[http://pcp.oxfordjournals.org/content/30/2/303.short] High productivity is achieved when ammonia is maintained at up to 50% of the total nitrogen.[http://www.sciencedirect.com/science/article/pii/S0960852410018213] The uptake of nitrate is light energy dependent, and the reduction of nitrite consumes energy.[http://www.sciencedirect.com/science/article/pii/S0306261910005623]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4484</id>
		<title>Composting toilet</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4484"/>
		<updated>2014-12-31T19:10:07Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[Image:Urine_diversion.jpg|thumb|left| urine diversion ]]&lt;br /&gt;
[[Image:Composting_toilet.jpg|thumb|right| [http://en.wikipedia.org/wiki/Composting_toilet#mediaviewer/File:Clivus_Multrum_Composting_toilet_with_urine_diversion-dehydration.svg Clivus Multrum] adapted for solar ventilation and urine re-use&lt;br /&gt;
- 1. Humus compartment 2. Ventilation inflow 3. WC 4. Urinal 5. Urine collection 6. Manual paddles 7. Solar heating of ventilation outflow ]]&lt;br /&gt;
[[Image:Urine_diversion_2.jpg|thumb|left| urine diversion - squatting [http://en.wikipedia.org/wiki/Urine-diverting_dry_toilet#mediaviewer/File:Inside_of_the_UDDT_(5332645379).jpg]]]&lt;br /&gt;
&lt;br /&gt;
It is estimated that the excreta from 80 persons could fertilize a hectare. A single adult eats 250 kg of cereals per year, which may be grown on less than 250 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;, fertilised by ~50% of the urine of that person, mixed with the waste water used by that person.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf]&lt;br /&gt;
&lt;br /&gt;
Composting toilets aerobically decompose waste material, by aerobic microorganisms in a highly aerated, warm (32°-49°) and moist (humidity &amp;gt;80%) environment, with ample carbon (sawdust as the sole agent will lead to clogging[http://www.lismore.nsw.gov.au/infocouncil/Open/2013/OC_13082013_ATT_EXCLUDED_WEB.HTM]). During composting, mineralization, and humification occur simultaneously and are the main processes leading to degradation of the fresh organic matter. Organic matter humification in composting occurs when lignin and its degradation products are degraded by the microorganisms.[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] Optimally, the volume of the resulting compost may be about 10% of input.[http://en.wikipedia.org/wiki/Composting_toilet] &lt;br /&gt;
&lt;br /&gt;
Continuous composting toilets are single container toilets that receive excrement which decomposes as it moves slowly through the container and is removed as compost from the end-product chamber. The container is permanently fitted under the toilet seat, and never has to be fully emptied as the compost can be gradually removed when it reaches the end-product chamber. [http://www.yourhome.gov.au/water/waterless-toilets] To make the fresh material move down the slope, the slope needs to be slippery, eg bathroom tiles. Natural zeolite may be used as medium for the sorption of ammonia from wastewater and subsequently as nitrogen releaser in cultures of Spirulina platensis.[http://www.sciencedirect.com/science/article/pii/S0960852413019627]&lt;br /&gt;
&lt;br /&gt;
In a non-urine-diverting system, 90% of what goes into a composting toilet is water. Compost piles need to be damp to work well, but most composting toilets suffer from too much water. (ideally 45 to 70% moisture content [http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets])&lt;br /&gt;
Ammonia may evaporate as nitrogen in a gas form, which is vented out along with carbon dioxide and water vapor.[http://www.google.com/patents/US4096592]&lt;br /&gt;
Evaporation is the primary way a composting toilet gets rid of excess water. Warmth and air flow through the unit assist the evaporation process. Every composting toilet has a vertical vent pipe to carry off moisture. Air flows across the drying trays, around and through the pile, then up the vent to the outside of the building.&lt;br /&gt;
In a cold environment, the low-grade heat produced by composting is supposed to provide sufficient updraft to carry vapor up the vent.[http://buttecreekwatershed.org/BMP/test2/Book6last.htm] In tropical conditions, passive solar heating may create an air draft.&lt;br /&gt;
&lt;br /&gt;
If operation conditions for thermophilic composting are adequate (moisture content 50 to 60%, carbon to nitrogen ratio 30 to 35 and mixing with bulking material), the temperature will rise to between 50 and 65 °C (WHO 2006). Such temperatures will effectively inactivate pathogens (WHO 2006).[http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets] If moisture content is too high, anaerobic organisms will thrive, creating undesirable odors from anaerobic digestion.&lt;br /&gt;
&lt;br /&gt;
==Carbon / Nitrogen ratio==&lt;br /&gt;
Dry material, which contains carbon (such as dry leaves or grasses) increases composting properties. It regulates moisture and the carbon to nitrogen ratio (C/N) and enhances the composting process. The optimal carbon to nitrogen ratio for thermophilic composting is 20 to 35 (WHO 2006). The C/N ratio of humanure (135 to 270 g / person / day) is about 5 to 10 (5%-7% nitrogen) The C/N ratio of grass clippings may be 12-19 and they may contain 2.4% nitrogen. The C/N ratio of leaves may be 48 and they may contain 0.9% nitrogen.[http://www.weblife.org/humanure/chapter3_7.html] Thus 200 g humanure plus 225 g leaves may render a combined C/N ratio of 30.&lt;br /&gt;
The C/N ratio of Panicum texanum is 32.9:1.[http://www.researchgate.net/publication/24186263_Nematode_Population_Fluctuations_during_Decomposition_of_Specific_Organic_Amendments] Thus 200 g humanure plus 1379 to 1724 g. [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Panicum Panicum texanum] may render a combined C/N ratio of 30.&lt;br /&gt;
&lt;br /&gt;
Crude protein in the leaves of Giant cane ([http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Arundinaria_gigantea Arundinaria gigantea]) is relatively low in young leaves.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=8259377&amp;amp;fileId=S1742170510000566] The C/N ratio in Giant cane leaves may be 19.4 (2.4% nitrogen and 45.8% carbon).[http://www.ars.usda.gov/SP2UserFiles/ad_hoc/19320000PosterGallery/AFGC2004_Bamboo_Final.pdf] Bamboo shoots may contain 0.6% nitrogen. Overall bamboo litter (culm, branches, leaves) may contain 1.4% nitrogen.[http://www.sciencedirect.com/science/article/pii/S0378112704006693] The carbon concentration in decomposing bamboo culms may be 50%, irrespective of decomposition level. Mean nitrogen concentration may be 1%.(going up from ~0.3% as decomposition advances) [https://www.deepdyve.com/lp/springer-journals/characteristics-of-culm-structure-and-carbon-and-nitrogen-pP0nfb7X0z/7] Bamboo biochar and wood biochar have the same C/N ratio[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] The C/N ratio of softwood may be 640, of hardwood may be 560 and that of sawdust 500, which goes down as it rots (down to 200).[http://www.weblife.org/humanure/chapter3_7.html] That is because the level of carbon (and Na, K) goes down faster than the level of nitrogen (and P).[http://link.springer.com/article/10.1007/BF00346060#page-1] Nitrogen is usually retained until 50% of the carbon remains.[http://link.springer.com/article/10.1007/s10021-004-0026-x#page-1] For the supply of carbon, one may process wood into sawdust, or one may let the chopped wood decompose naturally (with a lower C/N ratio). &lt;br /&gt;
&lt;br /&gt;
For a resulting combined C/N ratio of 30:&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 21 g                       bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 11 g                       bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 63 g  partially decomposed bamboo wood    (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 24 g  partially decomposed bamboo culms   (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 252 g partially decomposed bamboo culms   (if C/N=50) &lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 200 g partially decomposed bamboo culms   (if C/N=50)&lt;br /&gt;
&lt;br /&gt;
Fruit wastes may have a C/N ratio of 20-50.[http://www.norganics.com/applications/cnratio.pdf] Mango waste (without stone) may have a C/N ratio of 50.[http://www.researchgate.net/profile/DrLakshmi_priya_Thyagarajan/publication/225038970_Bioconversion_of_Mango_Waste_Blended_with_Poultry_Waste_and_Cow_Dung_into_Useful_Manure_by_Aerobic_Composting/links/0fcfd4fbfa557c43e1000000]&lt;br /&gt;
&lt;br /&gt;
==Urine==&lt;br /&gt;
[[Image:Urine-nutrients.jpg|thumb|left| Urine composition - a [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_3.jpg|thumb|right| Urine composition - c [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_2.jpg|thumb|left| Urine composition - b [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
Excessive ammonia from urine inhibits the microbial processes in the chamber. With urine diversion, less bulking agent is needed and the C/N ratio is naturally enhanced.&lt;br /&gt;
The C/N ratio of urine (1-2 L / person / day) is about 0.8 and its nitrogen content is ~15-18%.[http://www.weblife.org/humanure/chapter3_7.html]&lt;br /&gt;
Urine can contain up to 90 percent of the nitrogen, up to 50 percent of the phosphorus, and up to 70 percent of the potassium present in human excreta.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf] It may contain 9.3 g/L urea, 1.9 g/L chloride, 1.2 g/L sodium, 0.8 g/L potassium, 0.5 g/L phosphorus and 0.2 g/L ammonia.[http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf] &lt;br /&gt;
In healthy individuals it is usually pathogen free, although undiluted it may contain inorganic salts and organic compounds at levels toxic to plants. Ureum decomposes into ammonia, which smells.&lt;br /&gt;
&lt;br /&gt;
When applying urine to pastures, 14 to 28% of the nitrogen may evaporate in 2 weeks. 50% of that will be lost within the first 24 hours.[http://www.publish.csiro.au/?paper=AR9820097] The level of nitrogen volatilized as ammonia increases with increasing temperature.[http://link.springer.com/article/10.1007/BF00335848] Urine can be used as a source of complementary nitrogen in high carbon compost. When diluted with water at a 1:8 ratio, it can be used as a fertilizer [http://www.liquidgoldbook.com/], comparable to commercial fertilizers.[http://modernfarmer.com/2014/01/human-pee-proven-fertilizer-future/] &lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Algae_for_Tilapia#Spirulina Spirulina platensis] may be grown on human urine [http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] &lt;br /&gt;
At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g/L.[http://link.springer.com/article/10.1007%2FBF02179776#page-1] Since human urine typically contains about 9.3 gram urea per L [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf], 1 L of urine may be added to 5.2 L of clean water (or 161 ml of urine per L clean water).&lt;br /&gt;
Best cellular growth may occur at 500 mg/L of urea.[http://www.sciencedirect.com/science/article/pii/S0960852403002359] (53 ml urine per L clean water) Growth may be optimal when the urea feeding rate is slowly increased [http://www.sciencedirect.com/science/article/pii/S0044848604005952] and at 29°C. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] Best results are obtained by continuous urea addition in exponentially increasing amount, at 30°C.[http://www.sciencedirect.com/science/article/pii/S0961953402000545] Intermittent addition of urea yields results similar to those obtained by the continuous feeding.[http://www.sciencedirect.com/science/article/pii/S0144860904000263] Spirulina platensis may use several nitrogen sources; most effectively sodium nitrate, ammonium nitrate and urea.[http://link.springer.com/article/10.1023/A:1011925022941#page-1] Spirulina platensis grows better on urea than on potassium nitrate.[http://link.springer.com/article/10.1385/ABAB:112:3:143#page-1] Nitrate may yield a higher dry weight.[http://www.sciencedirect.com/science/article/pii/S0167299104803203] In combination with bicarbonates, urea may be more effective than ammonium and nitrate.[http://docsdrive.com/pdfs/academicjournals/ajpp/0000/47496-47496.pdf] Spirulina, when grown with urea as the nitrogen source, is characterized&lt;br /&gt;
by a higher methionine and arginine content.[http://link.springer.com/article/10.1007/BF00444067#page-1] Urea is the best source for increasing gamma-linolenic acid contents in Spirulina.[http://link.springer.com/article/10.1007/BF00218450#page-1] Though Spirulina platensis may tolerate high levels of urea better [http://link.springer.com/article/10.1007/s10295-005-0025-8#page-1], it is ammonium resistant at high pH.[http://pcp.oxfordjournals.org/content/32/7/953.short] The uptake of ammonia in Spirulina platensis (supporting optimal growth) is pH dependent with an optimum at pH 9.3, proceeding at the same rates in the light and in the dark.[http://pcp.oxfordjournals.org/content/30/2/303.short] High productivity is achieved when ammonia is maintained at up to 50% of the total nitrogen.[http://www.sciencedirect.com/science/article/pii/S0960852410018213] The uptake of nitrate is light energy dependent, and the reduction of nitrite consumes energy.[http://www.sciencedirect.com/science/article/pii/S0306261910005623]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4474</id>
		<title>Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4474"/>
		<updated>2014-12-31T16:54:44Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling,&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Feeding==&lt;br /&gt;
[[Image:Table_1.jpg|thumb|left| Table 1 for feeding tilapia [http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf © Alaa A. El-Dahhar]]]&lt;br /&gt;
[[Image:Chaoborus.jpg|thumb|right| Chaoborus [http://en.wikipedia.org/wiki/Glassworm#mediaviewer/File:GlasswormLateralView.JPG © Viridiflavus]]]&lt;br /&gt;
[[Image:Table_2.jpg|thumb|left| Table 2 for feeding tilapia [http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf © BFAR]]]&lt;br /&gt;
[[Image:Chironomus.jpg|thumb|right| Chironomus [http://www.thamesvalleybirds.co.uk/insect-invertebrate-macro-photographs/11156-non-biting-midge-male-probably-chironomus-plumosus.html © ladylouise62]]]&lt;br /&gt;
[[Image:Ceriodaphnia_reticulata.jpg|thumb|right| Water fleas [http://www.micromagus.net/microscopes/pondlife_cladocera.html © micromagus.net]]]&lt;br /&gt;
&lt;br /&gt;
Tilapia may survive on [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] or [http://www.waiwiki.org/index.php?title=Lemna_For_Fish duckweed] alone, but combined feeding results in higher growth rates. Feeding on algae. feeding most intensively occurs between 12.00 and 18.00, and in this time span the fish may consume over 3% of their bodyweight.[http://cabdirect.org/abstracts/20013100658.html;jsessionid=1F937711ACC14AC20505C4692DBC7CB3] Feed efficiency is highest at 2% BW.[http://www.sciencedirect.com/science/article/pii/S0044848697000392] Tilapia may also feed on insects / larvae [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2028.2004.00503.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false], such as water fleas (Ceriodaphnia &amp;lt; 1mm long) [http://link.springer.com/article/10.1007/BF00016658#page-1] (1&amp;gt; Daphnia &amp;lt;5 mm long; which cannot easily avoid predation) [http://www.int-res.com/articles/ame/25/a025p215.pdf] (or Daphniamenucoensis, which are halophylic), Chaoborus midge larva (&amp;lt; 2cm) and Chironomus midges.[http://www.nativefishlab.net/library/textpdf/17926.pdf] Superior growth is achieved by partial replacement with feed from animal origin, such as commercial fishfeed or insects (eg [http://www.waiwiki.org/index.php?title=Fruit_flies fruit flies]) and [http://www.waiwiki.org/index.php?title=Armyworm worms]. Water transparency contributes to the inclusion of insects in Nile tilapia diets.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Protein and energy of the animal-based foodstuffs are more available to Oreochromis niloticus than that of the plant-based foodstuffs.[http://www.sciencedirect.com/science/article/pii/0044848687902298] &lt;br /&gt;
&lt;br /&gt;
In outdoor tanks fecundity and final mean weight is higher in tilapia fed 3% bw compared to lower feeding rates.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.t01-1-00864.x/abstract] In adult tilapia, the optimal interval between feedings is 4 to 5 hours. At shorter intervals, eaten food will surpass the already filled stomach, and become waste. Fry may be fed 8 to 10 times a day.[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf] Energy retention is highest in juvenile Tilapia (av. 34 g.) fed 3 times daily. Protein retention is highest in fish fed 5 times daily.[https://evols.library.manoa.hawaii.edu/handle/10524/19120] Tilapia may fast up to a week with compensatory enhanced growth after refeeding.[http://www.tandfonline.com/doi/abs/10.1300/J028v18n03_02#.VEZlXvnV9JA]&lt;br /&gt;
&lt;br /&gt;
Table 1 (on the left) can be used by fish farmers to adjust feeding rate and frequency after determination of the hatchery constant by sampling and weighing the fish. The hatchery constant will depend on water quality, stocking density etc.[http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf]&lt;br /&gt;
Table 2 (on the left) is a schedule for the administration of commercial fish feed. Larger feed particle sizes are associated with lower fish growth rates.[http://www.sciencedirect.com/science/article/pii/S0044848610006332]&lt;br /&gt;
&lt;br /&gt;
==Protein==&lt;br /&gt;
&lt;br /&gt;
Protein requirement for maximum performance of tilapia larva is 35 -&amp;gt; 50%, decreasing with increasing fish size.[http://jn.nutrition.org/content/111/6/1001.long][http://www.pjbs.org/pjnonline/fin988.pdf][http://www.sciencedirect.com/science/article/pii/004484869290278S] In tilapia fry the feed conversion ratio and the highest protein growth rate are highest with a 45% crude protein diet, but the protein efficiency ratio and protein productive value was highest with the 25% crude protein diet. Fingerlings and advanced juveniles showed optimum growth performance with the 35% crude protein diet.[http://www.sciencedirect.com/science/article/pii/S0044848609008849] In young tilapia, out of artificial diets with dietary protein levels of 20%, 30%, 40% and 50%, best growth and food conversion efficiency was obtained with 40% dietary protein.[http://www.sciencedirect.com/science/article/pii/0044848688900075] Maximum food conversion efficiency and growth of young tilapia was obtained using the diet containing 30% protein (compared to 20%, 40% and 50% protein), decreasing with increasing protein levels.[http://www.sciencedirect.com/science/article/pii/0044848688900075]&lt;br /&gt;
Juvenile tilapia need 30-40% protein, while adult tilapia need 20-30% dietary protein for optimum performance.&lt;br /&gt;
Tilapia broodstock need 35-40% protein for optimum reproduction, spawning efficiency, and larval growth and survival.[http://www.sciencedirect.com/science/article/pii/S0044848696013658] [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1998.00206.x/abstract][http://www.sciencedirect.com/science/article/pii/S0044848602002211]. In tilapia average 62 g. weight and 18 cm long, there was no significant increase in growth rate with increasing dietary protein levels (from 25% to 30%).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Excess protein results in unionized ammonia in the environment.[http://www.sciencedirect.com/science/article/pii/S0044848609008849]&lt;br /&gt;
&lt;br /&gt;
Duckweeds grown in enriched water may contain 30 to 40% protein.[http://pubs.acs.org/doi/pdf/10.1021/jf60230a040] Wolffia arrhiza (dry weight) duckweed may contain up to 40% protein.[http://www.ncbi.nlm.nih.gov/pubmed/16232450] Lemna paucicostata dry mass protein contents may range from 26% to 45%.[http://www.sciencedirect.com/science/article/pii/0304377088900794] Lemna minor grown in swine lagoon wastewater contained 32% protein.[http://www.sciencedirect.com/science/article/pii/S0960852412012291]&lt;br /&gt;
&lt;br /&gt;
Green [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] (Chlorella vulgaris, Scenedesmus obliquus) and blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) may accumalate protein from 8 to 54% as available nitrogen increases. [http://www.sciencedirect.com/science/article/pii/S0031942200803040] Spirulina platensis contains 50 to 65% protein (10% is non-protein nitrogen)[http://pubs.acs.org/doi/abs/10.1021/jf00105a022], or even up to 71.9% protein.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2012.05303.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
Insect true protein contents may be reasonably estimated via crude protein (nitrogen x 6.25).[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Crude protein content of feeder insects (housefly, fly larvae, cockroach nymphs, tebo worms) may range from 15.5 to 19.7% [http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Total crude protein in dry matter is 32.5% in earth worms and 64.4% in adult crickets (water content &amp;gt;50%)&lt;br /&gt;
Protein contents of Armyworm (Spodoptera exempta) moths are similar in gregarious and solitary moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756]&lt;br /&gt;
&lt;br /&gt;
Silkworm (Bombyx mori) pupae are a rich source of high quality protein.[http://www.sciencedirect.com/science/article/pii/S0278691506000147] In silkworm larvae, two storage proteins may account for 60% of total fat body protein (80% of the soluble protein) in females, compared to only 20% of the total fat body protein in males.[http://www.sciencedirect.com/science/article/pii/0020179080900244] &lt;br /&gt;
&lt;br /&gt;
Mealworms (Tenebrio molitor) from commercial source contained 22.3% protein.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae but contained 76% protein on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847] Mealworms contain 53% (Tenebrio molitor) and 45% (Zophobas morio; &amp;#039;Superworm&amp;#039;) protein in dry matter.[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0051145] (Egg production in Tenebrio molitor is 640 eggs / female / year, and 1500 eggs / female / year in Zophobas morio) Yellow mealworms (Tenebrio molitor; 4.8-182.7 mg) fed on wheat flour and brewers yeast contained 24.3-27.6% protein.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] A 40% protein fish meal diet with up to 40% replacement of fish meal with mealworm (Tenebrio molitor) meal does not affect growth of catfish. Catfish fed diets with up to 80% replacement of fish meal with the worm meal still displayed good growth and feed utilization efficiency. [http://onlinelibrary.wiley.com/doi/10.1046/j.1355-557x.2001.00024.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Cuticle larval / pupal mealworm proteins are more water-soluble at low temperatures (4 to 10°C) and become more hydrophobic at 25°C.[http://www.sciencedirect.com/science/article/pii/S0965174802000450] These cuticle proteins (as in the locust) lack acidic amino acid residues, methionine, cysteine and tryptophan.[http://www.sciencedirect.com/science/article/pii/096517489400048M]&lt;br /&gt;
&lt;br /&gt;
The digestibility coefficients for protein in Oreochromis niloticus are: soybean meal (91%), fish meal (86%), ground corn (83%), wheat middlings (75%), poultry-offal meal (74%), and brewers grain (63%).[http://www.sciencedirect.com/science/article/pii/0044848687902298]&lt;br /&gt;
&lt;br /&gt;
Protease activity in Tilapia is relatively low, in comparison to Grass carp (Ctenopharyngododn idellus), Common carp (Cyprinus carpiol), silver carp (Hypophthaimichthys molitrix) and bighead carp (Aristichys nobilis Richardson).[http://en.cnki.com.cn/Article_en/CJFDTOTAL-BEAR199302007.htm] Alkaline protease activity in Oreochromis niloticus is significantly inhibited by the ingestion of soybean meal, corn gluten meal and wheat bran, in comparison to the sensitivity to protease inhibitors in seabream and sole.[http://www.sciencedirect.com/science/article/pii/S0305049199000243]&lt;br /&gt;
&lt;br /&gt;
==Fat==&lt;br /&gt;
&lt;br /&gt;
Tilapia do well on fat versus carbohydrates as a source of energy.[http://www.ncbi.nlm.nih.gov/pubmed/22004562] Excess dietary lipids, however, are readily stored in the carcass and the viscera, but are not utilized for improving growth or food utilization efficiency.[http://www.sciencedirect.com/science/article/pii/004484869190224U]&lt;br /&gt;
&lt;br /&gt;
Larvae of mealworms, crickets, waxworms and fruit flies (Drosophila melanogaster) on average have 30% of dry matter higher fat content than adult species.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Newly emerged Armyworm moths may (still) contain high levels of (glyceride [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1993.tb00462.x/abstract]) lipid (largely in the abdominal), the quantity depends on the larval feeding conditions. Both the Armyworm larvae and young adult moths have the capacity to accumulate lipid reserves in excess. Big female moths contain relatively much fat. The moths are able to supplement their lipid reserves following carbohydrate uptake.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1986.tb00433.x/abstract] Gregarious larva have 2.5 to 6.1 higher abdominal glyceride contents than solitaria phase moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756] The stored fat may serve as fuel for long migratory flights.[http://www.sciencedirect.com/science/article/pii/0305049188901484] In feeder insects (house fly, fly larvae, cockroach nymphs, tebo worms), crude fat may range from 1.9% to 29.4%.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Yellow mealworms (4.8-182.7 mg) fed on wheat flour and brewers yeast contained 12.0-12.5% fat.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] Mealworms (Tenebrio molitor) from commercial source contained 15% fat.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae and contained 6.4% fat on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847]&lt;br /&gt;
&lt;br /&gt;
Energy content for silkworms is 674 kcal/kg and 2741 kcal/kg for waxworms.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10031/abstract]&lt;br /&gt;
&lt;br /&gt;
In regard to gross energy, the digestibilities in Oreochromis niloticus are: animal oil (93%), fish meal (80%), ground corn (76%), poultry-offal meal (59%), wheat middlings (58%), soybean meal (56%), and brewers grain (30%).[http://www.sciencedirect.com/science/article/pii/0044848687902298] Replacing sunflower oil (69% omega-6; 0% omega-3) with perilla oil (50 to 60% ALA/omega-3) changed fatty acid profiles in Nile tilapia, which stabilized after 20 days under the new diet. Total omega-3 in muscle tissue fat increased from 6.4% to 18.2% (almost 3-fold), particularly due to a 9.4 fold increase in ALA levels.[http://www.ncbi.nlm.nih.gov/pubmed/24262550] Palm oil may replace soybean oil in feeds for Oreochromis niloticus fingerlings without any negative effect on growth or body composition.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1996.tb00988.x/abstract]&lt;br /&gt;
&lt;br /&gt;
Spirulina may contain 7% [http://www.ejbiotechnology.info/content/vol9/issue4/full/5/] to 14% fat (of dry mass)[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1]. At low nitrogen (N) levels, green algae (Chlorella vulgaris and Scenedesmus obliquus) contain 45% lipids of biomass, containing mainly 16:0 and 18:1 fatty acids. At high N levels, total lipids dropped to 20% of the dry weight, with elevated levels of polyunsaturated C16 and C18 fatty acids [[http://www.sciencedirect.com/science/article/pii/S0031942200803040] during the initial stages of growth. Towards the end of growth the main lipids contained mainly saturated fatty acids (mostly 18:1 and 16:0).[http://www.sciencedirect.com/science/article/pii/S0031942200803052] In blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) lipid composition was reported not to be affected by N concentrations [http://www.sciencedirect.com/science/article/pii/S0031942200803040], but by sodium-nitrate as well as by temperature.[http://www.znaturforsch.com/ac/v59c/s59c0055.pdf] The fatty acids 18:3n−3, 18:2n−6, 18:0, 18:1 and 16:0 dominate in microalgae (eg Chlorella vulgaris, Scenedesmus abundans, Monoraphidium minitum), but are highest in Chlorella vulgaris. This difference is not reflected in their predator, Brachionus calyciflorus, and in Tilapia feeding on Brachionus calyciflorus. The Tilapia zillii larvae contained relatively much 22:6n−3, which is indicative for the capacity to elongate and desaturate dietary linoleic and linolenic acid.[http://www.sciencedirect.com/science/article/pii/S0044848699000137]&lt;br /&gt;
&lt;br /&gt;
==Carbohydrates==&lt;br /&gt;
&lt;br /&gt;
Tilapia is relatively well equipped (due to alpha-Amylases[http://www.ncbi.nlm.nih.gov/pubmed/11250550] produced by Aeromonas, Bacteroidaceae and Clostridium strains[http://www.ncbi.nlm.nih.gov/pubmed/9081303]) for utilizing carbohydrates.[http://www.ncbi.nlm.nih.gov/pubmed/23168215] Tilapia can utilize up to 46% dietary starch without growth retardation.[http://www.researchgate.net/publication/240652906_Effects_of_dietary_carbohydrate_level_on_growth_and_body_composition_of_juvenile_tilapia_Oreochromis_niloticusxO._aureus] Tilapias do better on starch than on glucose (even when the glucose diet is supplemented with chromic oxide) [http://www.ncbi.nlm.nih.gov/pubmed/7722702] Starch consists of amylose and amylopectin. Amylopectin is more susceptible to enzymatic cleavage. A high-dietary amylose-amylopectin ratio (&amp;gt; 0.24; as in corn starch) decreases starch digestibility and postprandial peak blood glucose and triglycerides, and inhibits feed efficiency and growth in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Amylopectin accounts for about 75% of the starch weight in reserve starch (in storage organs) [http://www.sciencedirect.com/science/article/pii/S1360138598013429] and typically more than 90% in transitory starch (in photosynthetic organs)[http://www.ncbi.nlm.nih.gov/pubmed/12068097/]. Spirodela polyrrhiza amylose content was 21%[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 25% in potatoes[http://link.springer.com/article/10.1007/BF02849767#page-1], 7-44% in barley[http://ajcn.nutrition.org/content/59/5/1075.short] and 25–28 in wheat.[http://www.sciencedirect.com/science/article/pii/S0924224405003572] Feeds with smaller granule size have higher starch digestibility than those with bigger granules. Spirodela polyrrhiza granules range from 1 to 8 μ [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 2-55 μ in wheat starch [http://link.springer.com/article/10.1023/A:1022255309597#page-1], 7 to 22 µ in corn starch and 8 to 110 µ in potatoes [http://www.karger.com/Article/Abstract/196856].&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus weight gain, feed conversion, specific growth rate and protein efficiency ratio were improved with diets containing date (15%, 30% and 45%) as compared with the starch diet (0% date diet). The diet containing 30% date was superior to all other test diets in terms of all the above growth parameters, while body fat was reduced.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.00872.x/abstract] &lt;br /&gt;
&lt;br /&gt;
In tilapia, polysaccharides have antibacterial properties, increasing resistance to infections.[http://www.ncbi.nlm.nih.gov/pubmed/9280393] Spirulina platensis contains 13.6% carbohydrate, principally glucose along with rhamnose, mannose, xylose, galactose and two unusual sugars (2-O-methyl-L-rhamnose and 3-O-methyl-L-rhamnose).[http://www.sciencedirect.com/science/article/pii/S0031942200846981] Spirulina platensis contains various polysaccharides [http://www.morevollara.com/assets/downloads/two-Immunostimulatory-Activity-Pugh-2001.pdf], including starch.[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1] The amount of starch is low due to the high activity of α and β amylase in Spirulina.[http://www.ijpba.info/ijpba/index.php/ijpba/article/viewFile/894/607] In Spirulina platensis, the polysaccharide SP-4 consists of L-rhamnose, L-fucose, L-arabinose, D-glucose, D-glucoseamine and D-galacturonic acid. It enhances proliferation of lymphocytes.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GZHX199701006.htm] The polysaccharide PSP in Spirulina platensis is composed of L-fucose, D-mannose, D-galactose, D-glucose and glucuronic acid.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-DLYY503.001.htm] Another polysaccharide in Spirulina platensis containes glucose, principally, with small amounts of sulphate; a glucan backbone with glucosyl sidechains.[http://www.sciencedirect.com/science/article/pii/0308814689900198] Some Spirulina polysaccharides have antiviral properties.[http://online.liebertpub.com/doi/abs/10.1089/aid.1996.12.1463][https://www.jstage.jst.go.jp/article/cpb/49/1/49_1_108/_article] Spirulina platensis also secretes polysaccharides.[http://link.springer.com/article/10.1007/BF00138541#page-1] The cell wall of Spirulina platensis lacks cellulose (and is therefore readily digested).[http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8?LI=true#page-1]&lt;br /&gt;
&lt;br /&gt;
The cell walls of green algae (Chlorophyta) contain cellulose (and pectose, the outer layer) and carbohydrates are stored as starch. Mealworms (Tenebrio molitor) from commercial source contained 3.6% carbohydrates.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503]&lt;br /&gt;
&lt;br /&gt;
==Chito-oligosaccharides==&lt;br /&gt;
&lt;br /&gt;
In tilapia stomach, intestine, and serum, chitinases are present. [http://www.ncbi.nlm.nih.gov/pubmed/17126584] Chitinases are enzymes required to enzymatically decompose chitin. Chitin is an amino polysaccharide; a long chain of many monosaccharide (N-acetylglucosamine) units. A chito-oligosaccharide is a short chain of just a few linked units of N-acetylglucosamine. Chitin may be split into chito-oligosaccharides. Chitin plus other composite materials (eg glycoproteins, calcium carbonate) forms the exoskeletons of insects and the flexible body wall of larva / caterpillars (eg silkworms[http://www.sciencedirect.com/science/article/pii/S0144861705005412] and fruit flies[http://www.ncbi.nlm.nih.gov/pubmed/807670]). Apparent digestibility coefficient of chitin ingested by Oreochromis niloticus from crayfish exoskeleton meal was 69.3%.[http://www.sciencedirect.com/science/article/pii/S0044848604007148] The peritrophic matrix of the tobacco hornworm (Manduca sexta) may contain 40% chitin.[http://www.sciencedirect.com/science/article/pii/0965174895000534] (Peritrophic matrices usually contain 3 to 13% chitin[http://jeb.biologists.org/content/206/24/4393.full]) Wild-caught earthworms may contain 51% chitin of dry matter.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Crustaceans may contain 12 to 20% chitin.[http://business.highbeam.com/435986/article-1G1-328419079/chitin-extraction-crustacean-shells-using-biological] Arthropod species (eg crustaceans, arachnids) with a chitin content of over 50% (of total weight) are eliminated unchanged by Tilapia zillii [http://www.sciencedirect.com/science/article/pii/0044848678900157] Insects may contain 0.3 to 5% chitin. (1.2 to 14% of dry matter)[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Chitin is also an ingredient of cell walls in algae.[http://www.ncbi.nlm.nih.gov/pubmed/3910139][http://www.ncbi.nlm.nih.gov/pubmed/23559820] Dietary intake of chito-oligosaccharides can improve intestinal health, and improve tilapia resistance to infection.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Dietary intake of chito-oligosaccharides at up to 4 g/kg may considerably improve growth, survival and immune response.[http://wenku.baidu.com/view/6da4fdd250e2524de5187ec7.html] Dietary intake of chito-oligosaccharides at 3 to 5 g/kg for juvenile GIFT tilapia may increase growth performance, nonspecific immunity and promote blood lipid metabolism.[http://www.agrpaper.com/applied-research-of-chito-oligosaccharide-on-gift-tilapia-oreochromis-niloticus.htm]&lt;br /&gt;
&lt;br /&gt;
==Vitamins &amp;amp; Minerals==&lt;br /&gt;
Oxalic acid is an anti-nutrient, reducing nutrient conversion in tilapia. High calcium available to duckweeds results in higher duckweed oxalic acid contents.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2004.00923.x/pdf] Lower duckweed-calcium may be compensated for by higher calcium in worms fed to tilapia. In mealworms supplemented with Ca from CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; (4 to 12%), the Ca content of the mealworms increased linearly with the Ca content of the substrate during the first 24 hours and decreased after over one week, especially at the higher levels of Ca supplementation. The Ca in these mealworms was 76% as bioavailable. [http://www.bioone.org/doi/abs/10.1638/1042-7260(2000)031%5B0512:ITCCOM%5D2.0.CO%3B2] Ca concentrations of silkworms also increased in a linear fashion with increasing levels of dietary Ca. Gut-loading diets for mealworms should be supplemented to contain 9% calcium, iron (51 mg/kg) and manganese (31 mg/kg). Gut-loading diets for silkworms should be supplemented to contain 23 g calcium per kg feed.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10082/abstract]&lt;br /&gt;
&lt;br /&gt;
Insects (mealworms, waxworms, crickets, fruitflies) on average have low calcium concentrations (0.11%), but sufficient concentrations of Cu, Fe, Mg, P, and Zn to meet known requirements of domestic birds and mammals. Supermealworms (Zophobas morio) and waxworms contain deficient levels of manganese. Earthworms meet all dietary mineral requirements. [http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract]&lt;br /&gt;
&lt;br /&gt;
Intestinal microorganisms in Tilapia nilotica produce at least 11.2 ng of vitamin B12 per g of body weight per day. In 16-weeks without Dietary B12, fish weight increased 8-fold (normal growth), and liver-stored B12 did not decrease.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1982)111%3C485%3AISADNO%3E2.0.CO%3B2#.VCvqCfl_s10] Magnesium absorption from the gastrointestinal tract may be highly efficient in Oreochromis mossambicus.[http://www.ncbi.nlm.nih.gov/pubmed/24194247]&lt;br /&gt;
&lt;br /&gt;
==Supplements==&lt;br /&gt;
[[Image:Allspice.jpg|thumb|right| Allspice [http://en.wikipedia.org/wiki/Allspice#mediaviewer/File:AllspiceBowl.JPG © Jonathunder]]]&lt;br /&gt;
[[Image:Babysbreath.jpg|thumb|right| Baby&amp;#039;s breath [http://en.wikipedia.org/wiki/Gypsophila_paniculata#mediaviewer/File:Gypsophila_paniculata.jpg © PiPi]]]&lt;br /&gt;
&lt;br /&gt;
* Saponin-rich plants such as Quillaja saponaria (Soap bark tree), yucca and Sapindus saponaria (wingleaf soapberry, western soapberry, jaboncillo) may increase growth, increase the nce male to female ratio in tilapia [http://www.ncbi.nlm.nih.gov/pubmed/22444893] and increase serum cholesterol in males.[http://www.sciencedirect.com/science/article/pii/S1532045602001679] Gypsophila paniculata (baby&amp;#039;s breath) and Quillaja saponaria (both high in saponins) may induce release of LH (Luteinizing hormone)[http://www.ncbi.nlm.nih.gov/pubmed/15792626] Supplementing Tilapia fry with 700 mg/kg extracted Quillaja saponin extract resulted in significantly increased growth, higher number of males, higher LH release and higher serum cholesterol.[http://www.ncbi.nlm.nih.gov/pubmed/12458187] Saponin supplementation diminishes egg production by females.[http://www.ncbi.nlm.nih.gov/pubmed/11423383] Intestinal magnesium uptake in tilapia is stimulated by chloride and inhibited by saponins (which may induce intravesicular magnesium accumulation.[http://www.ncbi.nlm.nih.gov/pubmed/8952951] In tilapia, saponins selectively permeabilize the plasma membrane.[http://www.ncbi.nlm.nih.gov/pubmed/24194248]&lt;br /&gt;
* Allspice Pimenta dioica (10 g/kg fish) acts as a growth promoter to improve feed utilization and weight gain in Mozambique Tilapia fry and acts an antimicrobial agent to enhance disease resistance during first feeding of fry.[http://www.ncbi.nlm.nih.gov/pubmed/25229484] Allspice (aka Jamaica pepper, myrtle pepper, pimenta, pimento, English pepper, newspice) is dried unripe berries of Pimenta dioica.&lt;br /&gt;
* Honey bee pollen (2.5% (w/v)) increases growth performance parameters, feed efficiency ratio and immunological, hematological and biochemical parameters in young Nile tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25086230]&lt;br /&gt;
* A supplemental mixture (at 0.5 to 2% w/w) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle may increase lysozyme, superoxide dismutase and peroxidase activity, decrease malondialdehyde levels and reduce mortality in Aeromonas hydrophila-challenged tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24925761] 20 days administration of 1% Astragalus root (Radix astragalin seu Hedysari) plus Angelica Root (Angelicae Sinensis) at a ratio of 5:1 (w/w) increased body weight of carp relative to controls.[http://www.ncbi.nlm.nih.gov/pubmed/15123322] Another study found no effect on growth by an mixture of Astragalus membranaceus, orange peel, hawthorn, pilose asiabell root indigowoad root, taraxacum and malt.[http://www.ncbi.nlm.nih.gov/pubmed/18378466] Astragalus (aka milkvetch, locoweed) belongs to the legume family Fabaceae. Dietary Astragalus polysaccharides supplementation may improve growth performance and immune parameters of tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24657260] Calycosin is a major isoflavonoid extracted from astragalus. It has shown to have proangiogenic effects.[http://www.ncbi.nlm.nih.gov/pubmed/21909574] Two isomeres extracted from Astragalus membranceus may stimulate proliferation in human fetal lung diploid fibroblast cells.[http://www.ncbi.nlm.nih.gov/pubmed/14659591] &lt;br /&gt;
* Survival in Streptococcus agalactiae challenged tilapia is increased by inclusion of 0.1% Sophora flavescens (root extract) in the diet.[http://www.ncbi.nlm.nih.gov/pubmed/23092731] Sophora flavescens (member of the Fabaceae family) roots contain quinolizidine alkaloids, including matrine, oxymatrine and lupeol. Matrine inhibits cell proliferation [http://www.ncbi.nlm.nih.gov/pubmed/24653570] (including proliferation of cancer cells)[http://www.ncbi.nlm.nih.gov/pubmed/24137393][http://www.ncbi.nlm.nih.gov/pubmed/24137358] Matrine prevents cardiac arrhythmias by inhibiting ouabain-induced calcium overload in guinea pigs.[http://www.ncbi.nlm.nih.gov/pubmed/24680622] Matrine has neurotoxic properties in Zebrafish (Danio rerio) larvae.[http://www.ncbi.nlm.nih.gov/pubmed/24911943]&lt;br /&gt;
&lt;br /&gt;
==Dissolved oxygen==&lt;br /&gt;
Fish take up oxygen through the limited gill surface area. At low dissolved oxygen (DO), more metabolic energy is used by the ventilatory system to maintain O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; uptake, which limits weight gain and growth.[http://www.sciencedirect.com/science/article/pii/0044848694902364] Feed intake and growth at 5.6 ppm (mg/L) DO is significantly higher than at 3.0 ppm, particularly in big fish.[http://www.sciencedirect.com/science/article/pii/S0044848608000021] For fish over 200 grams, feed intake continuous to increase over 3.0 ppm DO (with 5.5 ppm being the incipient DO), but digestibility is higher at suboptimal DO.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2011.02882.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
DO levels in ponds increase during the night due to vertical circulation. Organic materials consume oxygen during decomposition.[http://deepblue.lib.umich.edu/bitstream/handle/2027.42/27066/0000056.pdf?sequence=1] DO levels sharply decline after feeding. Dissolved oxygen levels should be maintained above 5.0 ppm for best growth. At DO levels between 3.0–5.0 ppm feeding should be reduced, and feeding should be stopped at DO levels below 3.0 ppm. Low DO increases the toxicity of ammonia (NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; unionized ammonia &amp;gt; 2.0 mg/L is lethal for tilapia, growth is inhibited below 1.0 mg/L). &lt;br /&gt;
[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf]&lt;br /&gt;
Under recirculated water conditions, mean body weight of Oreochromis aureus was higher at 6.5 ppm DO (mg/L), but food conversion ratio was best at 3.7 ppm DO.[http://www.sciencedirect.com/science/article/pii/0144860995000135]&lt;br /&gt;
Intermediate densities of phytoplankton produce higher dissolved oxygen concentrations. Dissolved oxygen levels may be raised by increasing algal growth, not necessarily by reducing algal biomass.[http://www.sciencedirect.com/science/article/pii/0044848688903572] DO concentrations at dawn is not related to manure input.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract] Low DO increases mortality due to Streptococcus infection.[http://www.sciencedirect.com/science/article/pii/S004484860000346X]&lt;br /&gt;
&lt;br /&gt;
==Salinity==&lt;br /&gt;
[[Image:Saisola_soda_3.jpg|thumb|left| Salsola soda [http://www.invasive.org/browse/detail.cfm?imgnum=5374910 © Joseph M. DiTomaso]]]&lt;br /&gt;
[[Image:Saisola_soda_2.jpg|thumb|right| Salsola soda [http://www.magicgardenseeds.com/SAL02 © MagicGardenSeeds]]]&lt;br /&gt;
[[Image:Saisola_kali_3.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_3.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Saisola_kali_2.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_2.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus may grow better in 7.5 ppt than in 0 ppt salinity [http://www.sciencedirect.com/science/article/pii/S0044848605000888] and may fairly tolerate salinity up to 10 ppt [http://www.sciencedirect.com/science/article/pii/0044848685901024] Tilapia may tolerate 0.5% salinity (5 ppt).[http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf] &lt;br /&gt;
At 8 g / L (8 ppt) salinity adult Oreochromis niloticus may be stocked at 40 fish / m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;. At 15 g/L (15 ppt) salinity total production is more than halfed.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2006.01605.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
Energy required by Oreochromis niloticus for osmoregulation is the least in the absence of an osmotic gradient (11.6‰ salinity) [http://www.nrcresearchpress.com/doi/abs/10.1139/f69-277#.VEY_ufnV9JA] In freshwater, heating water to temperatures above 27°C is not justifiable, while at 18 or 36 ppt salinity, heating water to 32°C can maximize growth rates without lowering growth efficiency.[http://www.sciencedirect.com/science/article/pii/004484869390392C] &lt;br /&gt;
&lt;br /&gt;
Oreochromis mossambicus tend to have lower growth rates than Oreochromis niloticus.&lt;br /&gt;
Oreochromis mossambicus is euryhaline and grows more rapidly in brackish water. (50% seawater)[http://www.ncbi.nlm.nih.gov/pubmed/11248987] Seawater salinity is 35 ppt.&lt;br /&gt;
Oreochromis mossambicus reared in salt water grows significantly larger than fish reared in fresh water. [http://www.sciencedirect.com/science/article/pii/S1096495903002458] This may be due to a decrease in the resting metabolic rate (which decreased with increasing fish size).[http://www.sciencedirect.com/science/article/pii/0044848695010130] Increasing dietary energy concentration increased mortality in Oreochromis mossambicus. The optimum protein to energy ratio for rapid growth, efficient feed conversion and maximum retention of protein and energy appears to be approximately 23.8 mg of protein per kJ energy.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1995.tb00935.x/abstract]&lt;br /&gt;
&lt;br /&gt;
Oreochromis mossambicus (♂) × Oreochromis niloticus (♀) may produce 100% male offspring (&amp;#039;red hybrid tilapia&amp;#039;) [http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0471048267.html](p.363), but spawning frequency may be very low [http://www.sciencedirect.com/science/article/pii/S0044848605000888] and infiltration of parental broodstock by hybrids may be difficult in mass production.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1994.tb00743.x/abstract]&lt;br /&gt;
Oreochromis mossambicus (♀) × Oreochromis niloticus (♂) hybrids are relatively salinity tolerant. Median Lethal Salinity (50% survival in 96 h after transfer from freshwater) ranged from 17.2‰ at 30 days post-hatching to 26.7‰ at 60 days post-hatching.[http://www.sciencedirect.com/science/article/pii/0044848685902200] This may be due to Morphological changes in the oesophageal epithelium.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1988.tb05352.x/abstract] The hybrid is superior to Oreochromis mossambicus at all salinities and to Oreochromis niloticus at salinities above 10 ppt.[http://www.sciencedirect.com/science/article/pii/S0044848605000888] This hybrid may be deprived of food during one week in a 8 weeks period without negatively affecting growth.[http://www.sciencedirect.com/science/article/pii/S0044848600003537] Due to starvation, the percentage of polyunsaturated fatty acids (PUFA) increases significantly in these hybrids.[http://www.sciencedirect.com/science/article/pii/S0044848697000355] Growth rate, conversion efficiency and maximum food consumption in Red Hybrid Tilapia may be optimal at 28°C.[http://europepmc.org/abstract/MED/11767691]&lt;br /&gt;
&lt;br /&gt;
==Alkalinity &amp;amp; pH==&lt;br /&gt;
&lt;br /&gt;
Alkalinity is the &amp;quot;buffering&amp;quot; capacity of water to resist a change in pH (pH fluctuations). To increase pH, add sodium carbonate (Na&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;) or calcium carbonate (CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;). Sodium carbonate may be extracted (as Barilla) from the ash of plants growing in sodium-rich soils, such as Halogeton sativus (aka Salsola sativa), Salsola soda and Salsola kali. The harvested plants are dried and subsequently burned. From this ash, the (water soluble) sodium carbonate is extracted with water. This solution is boiled dry, resulting in &amp;quot;barilla&amp;quot;.[http://en.wikipedia.org/wiki/Barilla] Calcium carbonate is the main component in snailshells and eggshells, and present in rocks (particularly limestone, chalk, marble and travertine).&lt;br /&gt;
&lt;br /&gt;
To increase alkalinity, one may add sodium bicarbonate. To create sodium bicarbonate, sodium carbonate may be dissolved in water and treated with carbon dioxide. Solid sodium bicarbonate will precipitate.&lt;br /&gt;
To increase the alkalinity of 10.000 L water with 10 ppm, add 168 g. of sodium bicarbonate.[http://www.expertpool.biz/ph_alkalinity.htm]&lt;br /&gt;
Adding sodium carbonate or calcium carbonate decreases the level of free ammonium ions.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
The appropriate levels for aquaculture are 20-150 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L alkalinity and 7.4-8.2 pH.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
In general, culture waters with pH below 7.0 and total alkalinity below 50 mg /L will get benefits by liming.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] The withdrawal of CO2 by microalgae turns carbonate and bicarbonate ions into the major forms of inorganic carbon in water and increases its alkalinity. Sodium carbonate is more effective than calcium carbonate to increase water alkalinity. Adding sodium carbonate to water with 20 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity, increasing alkalinity up to 77 meq/L, does not affect Oreochromis niloticus growth rates. Calcium carbonate is better for tilapia growth, as increasing water hardness as well.[http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/download/8510/8510] Water alkalinity of 35 to 53 mg calcium carbonate / L water is optimum for larval tilapia growth.[http://www.ablimno.org.br/acta/pdf/acta_limnologica_contents1604E_files/Art4_16(4).pdf] For optimum Oreochromis niloticus growth, the total hardness/total alkalinity ratio needs to be greater than 1.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] The best conditions for Oreochromis niloticus growth are 7.4-8.2 pH; total alkalinity &amp;gt; 50 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L; calcium hardness &amp;gt; 140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;/L and free CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; &amp;lt; 7 mg/L.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
Total hardness refers to the content of soluble Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; and Mg&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; in the water. Tilapia need to absorb these ions from the gills for several physiological functions. When total alkalinity is much greater than hardness, water pH may reach 11 in the mid-afternoon due to photosynthesis, because more free CO&amp;lt;sup&amp;gt;2-&amp;lt;/sup&amp;gt;&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; remains in solution.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
==Water flow==&lt;br /&gt;
A flow rate of 0.5 L/min per kg biomass appears to be desirable for intensive culture of tilapia.[http://www.sciencedirect.com/science/article/pii/004484869190073G]&lt;br /&gt;
&lt;br /&gt;
==Stocking density==&lt;br /&gt;
Tilapia needs to be stocked by age group to prevent cannabalism. The risk of cannabalism increases with increasing size differences.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA465&amp;amp;dq=algae+tilapia&amp;amp;ots=BVjAbOF-zV&amp;amp;sig=aEIMIY5ekRp5adhtpwsOIqPQTMQ#v=onepage&amp;amp;q=algae%20tilapia&amp;amp;f=false] Given reasonable assumptions about production costs, the optimal final density of Oreochromis niloticus in a tank-based flow-through system is 73.7 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.ncbi.nlm.nih.gov/pubmed/23915501]&lt;br /&gt;
Combined net yield of both caged and open-pond tilapia was highest in the treatment with 50 large fish (av. 141 g.) per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.sciencedirect.com/science/article/pii/S0044848696013774]&lt;br /&gt;
Broodstock may be best stocked at 4 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1999.00311.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
Red tilapia stocked in a pulsed-flow aquaculture system at densities of 10 and 20 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; had significantly higher individual weights than fish stocked at 30, 50, and 70 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;, due to limiting water quality; ammonia concentrations may be high (4 mg/L), and dissolved oxygen low (2 mg/L).[http://www.tandfonline.com/doi/abs/10.1300/J028v05n01_08#.VEY9SvnV9JA] Oreochromis niloticus fry may grow optimally when stocked at 5 fry / L and fed at 30% bodyweight / day.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2002.00700.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Oreochromis niloticus fingerlings may be stocked at a density of 42.6/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; with a production of 18–20 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; in 210 days at a water flow rate of 1 L/min/kg fish biomass.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1989.tb00440.x/abstract] With dissolved oxygen levels below 2 ppm (or even below 1 ppm) most of the time, stocking 3 fish per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; was more profitable due to increased mortality and lower growth rates at higher stocking densities (6 and 9 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;), despite similar water qualities.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/487.pdf]&lt;br /&gt;
&lt;br /&gt;
==Growth==&lt;br /&gt;
Tilapia nilotica fingerlings grow optimally on diets containing 30-40% protein, 12-15% lipids, and 30-40% digestible carbohydrate.[http://ir.kagoshima-u.ac.jp/bitstream/10232/15661/1/AN00181784_v6-1_p56-71.pdf]&lt;br /&gt;
Where extra protein (from 25% to 30%) did not increase growth in young tilapia (av. 18 cm long, 62 g. weight), increasing feeding levels to 2% of bodyweight / day did.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
juvenile Oreochromis niloticus grow better at 26 and 30°C, than at 22 and 34°C.[http://www.sciencedirect.com/science/article/pii/S0306456507001118] Feed efficiency and protein efficiency ratio are better and juvenile fish grow better at 28°C than at 22°C and 34°C. Liver gluconeogenesis and lipogenesis are increased by low temperature (22°C).[http://www.ncbi.nlm.nih.gov/pubmed/24556257] Growth and feed utilization efficiency is similar in genetically improved farmed tilapia (GIFT), genetically male Nile tilapia (GMNT) and conventional Nile tilapia (CNT).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2007.01679.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Large Oreochromis niloticus (av. 288 g.) grow less in recirculating aquaculture systems compared to flow-through systems. The opposite is true for small fish (av. 81 g.)[http://www.sciencedirect.com/science/article/pii/S0044848609008254]&lt;br /&gt;
&lt;br /&gt;
The use of tuna by-product meal as a total replacement for fish meal into tilapia diets increases oxidative stress.[http://www.ncbi.nlm.nih.gov/pubmed/25139749]&lt;br /&gt;
&lt;br /&gt;
Higher energy conservation is achieved when fish are exposed to longer rather than shorter photoperiod cycles.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00450.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
If reproduction can be delayed in the females, average growth rates comparable to those of an all-male population may be achieved.&lt;br /&gt;
[http://www.sciencedirect.com/science/article/pii/004484869390034V]&lt;br /&gt;
&lt;br /&gt;
==Strains==&lt;br /&gt;
&lt;br /&gt;
Compared to Oreochromis niloticus strains from Egypt and Senegal (bred from wild collected stocks), and ‘Israel’, ‘Singapore’, ‘Taiwan’ and ‘Thailand’ (strains maintained for aquaculture purposes), a strain bred in Ghana from wild collected stocks showed a significantly lower body weight at 210 days.&lt;br /&gt;
[http://www.sciencedirect.com/science/article/pii/004484869390034V]&lt;br /&gt;
&lt;br /&gt;
==Masculinization==&lt;br /&gt;
Estrogen synthesis is crucial for ovarian differentiation, and transcription of the aromatase gene can be proposed as a key step in that process in fish. Treatments with an aromatase inhibitor (ATD, 1,4,6- androstatriene-3-17-dione) result in 75.3% masculinization of an all-female (XX) population in tilapia (dosage 150 mg/kg of food). The effectiveness of the aromatase inhibition by ATD is demonstrated by the marked decrease of the gonadal aromatase activity in treated animals versus control.[http://www.ncbi.nlm.nih.gov/pubmed/10471475]&lt;br /&gt;
&lt;br /&gt;
Aromatase activity as a key factor in sexual differentiation in Oreochromis niloticus.[http://www.ncbi.nlm.nih.gov/pubmed/11424212] The most sensitive time to aromatase inhibitors lies in the first week (between 7 and 14 days post hatch). Treatment with the aromatase inhibitor Fadrozole (nonsteroidal) showed a dose-dependent increase in the percentage of males from 0 to 200 mg per kg. At higher doses (200 to 500 mg / kg), the percentage of males remained more or less constant (92.5-96.0%).[http://www.ncbi.nlm.nih.gov/pubmed/10861549]&lt;br /&gt;
&lt;br /&gt;
The masculinizing actions of 17alpha-methyltestosterone (MT) are most potent at up to day 20 of age.[http://www.ncbi.nlm.nih.gov/pubmed/11694764]&lt;br /&gt;
&lt;br /&gt;
Treating female tilapia Oreochromis niloticus with methyltestosterone (at a dose of 50 mcg/g diet) resulted in 100% masculinization.[http://www.ncbi.nlm.nih.gov/pubmed/16115634] Fry may be fed a commercial diet (SRT-95) with 30 ppm α-methyltestosterone for 21 days to yield 99% males.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA183&amp;amp;dq=stocking+density+oreochromis+niloticus+flow-through&amp;amp;ots=BVjC9QG1uV&amp;amp;sig=oZEoAvtMefOYOjPjqXQ-tbd_z_w#v=onepage&amp;amp;q&amp;amp;f=false]&lt;br /&gt;
&lt;br /&gt;
Treatment with tamoxifen and letrozole (200mg/kg feed) to fingerlings of O. niloticus for 60 days brought about 98.5% masculinization.&lt;br /&gt;
Treatment with 17α methyltestosterone (35 mg/kg feed) to fingerlings of O. mossambicus after 8 days post hatch for 60 days obtained 100% sex reversed males with excellent growth.[http://www.ncbi.nlm.nih.gov/pubmed/23063432]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. The critical period for elevated-temperature-induced masculinization lies between days 10 and 15 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] Higher temperatures (during 5 days) before they are 5 days old induces deformities. Masculinization is induced at elevated temperatures (28 to 32°C) during 5 days after 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577] The advantage of producing faster growing males of Nile tilapia at high temperature would hardly compensate the loss of production incurred during the masculinising treatment.[http://www.sciencedirect.com/science/article/pii/S004484860000452X] High-temperature (almost 36.90 °C) treatments yield a significantly higher proportion of males (64.20–80%) with lower survival rates (60–81%). The sex ratio of progenies reared at temperature below 36°C never deviated significantly from the balanced sex ratio.[http://www.sciencedirect.com/science/article/pii/S0306456507001118]&lt;br /&gt;
&lt;br /&gt;
==Feminization==&lt;br /&gt;
The critical period for low-temperature-induced feminization lies between days 5 and 10 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] The period of maximal feminizing action of 17beta-estradiol (E(2)) upon sex ratio is before 10 days posthatching in tilapia (Oreochromis mossambicus).[http://www.ncbi.nlm.nih.gov/pubmed/11694764]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. Higher temperatures (during 5 days) before they are 5 days old induces deformities. Gonadal feminization is induced at lower temperatures (20°C) for 5 days before 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577]&lt;br /&gt;
&lt;br /&gt;
==Parasites==&lt;br /&gt;
Tilapia may be infected with: &lt;br /&gt;
&lt;br /&gt;
* Nematodes / roundworms, such as Contracaecum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Contracaecum multipapillatum [http://www.ncbi.nlm.nih.gov/pubmed/24573461]), Paracamallanus cyathopharynx, Procamallanus laevionchus [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Rhabdochona esseniae [http://www.ncbi.nlm.nih.gov/pubmed/7596571], Rhabdochona paski [http://www.ncbi.nlm.nih.gov/pubmed/23595492], Gnathostoma spinigerum, Gnathostoma doloresi and Gnathostoma hispidum.[http://www.ajtmh.org/content/58/3/316.long] Human gnathostomiasis infection is related to ingestion of &amp;quot;ceviche&amp;quot;.[http://www.ncbi.nlm.nih.gov/pubmed/2588073]&lt;br /&gt;
* Cestodes (aka tapeworms), such as Amirthalingamia sp. (eg Amirthalingamia macracantha; higher prevalence and intensity in wet season [http://www.ncbi.nlm.nih.gov/pubmed/21972071]), and Cyclustera sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Cyclustera magna [http://www.ncbi.nlm.nih.gov/pubmed/15357392]) &lt;br /&gt;
* Trematodes / flukes (encysted metacercariae)[http://www.ncbi.nlm.nih.gov/pubmed/19795754], such as Clonorchis sinensis (human liver trematode)[http://www.ncbi.nlm.nih.gov/pubmed/22471793], Tylodelphys sp.[http://www.ncbi.nlm.nih.gov/pubmed/24407916], Stictodora tridactyla (in brackish-water fish)[http://www.ncbi.nlm.nih.gov/pubmed/2332641], Ribeiroia marini [http://www.ncbi.nlm.nih.gov/pubmed/24996], Clinostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] ((eg Clinostomum Complanatum, Clinostomum tilapiae, Euclinostomum hetereostomum [http://www.ncbi.nlm.nih.gov/pubmed/23113215]), Diplostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Neascus sp, Acanthostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Bolbophorus sp.[http://www.ncbi.nlm.nih.gov/pubmed/21972071], Haplorchis yokogawai [http://www.ncbi.nlm.nih.gov/pubmed/21073146], Haplorchis pumilio, Haplorchis taichui, Centrocestus formosanus, Stellantchas musfalcatus, Echinochasmus japonicus [http://www.ncbi.nlm.nih.gov/pubmed/18564743], Heterophyes heterophyes, Heterophyes aequalis, Pygidiopsis genata, Stictodora sp., Phagicola sp.[http://www.ncbi.nlm.nih.gov/pubmed/18339484] (eg Phagicola ascolonga[http://www.ncbi.nlm.nih.gov/pubmed/21073146]) and Prohemostomumn vivax.[http://www.ncbi.nlm.nih.gov/pubmed/18383799] These trematodes loose their viability after 48 hours at -5°C.[http://www.ncbi.nlm.nih.gov/pubmed/19795748] The conditions of gas supersaturation (gas pressure &amp;gt; 111.2% saturation; N supersaturation, O2 undersaturation) may result in heavy monogenetic trematode infections in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/9234554] In Thailand, fish-borne zoonotic trematode (FZT) metacercarial infections were found in Nile tilapia from cage (2.5%) and pond aquaculture systems (10%) and in wild caught fish.(53%) [http://www.ncbi.nlm.nih.gov/pubmed/24029716] In China, tilapia from nursery and grow-out ponds were sampled from monoculture, polyculture and integrated aquaculture systems, revealing a 1.5% prevalence of FZT infections (Heterophyidae and Echinostomatidae); lower than in wild caught fish. Integrated systems using animal manure and latrine wastes as fertilizer did not show a higher prevalence.[http://www.ncbi.nlm.nih.gov/pubmed/24018184] In Vietnam, the overall FZT prevalence in tilapia from wastewater ponds was 2.0%, but much higher in tilapia from farm ponds. [http://www.ncbi.nlm.nih.gov/pubmed/22471793]&lt;br /&gt;
* Monogenea (very small flatworms), such as Dactylogyrus minutus [http://www.ncbi.nlm.nih.gov/pubmed/24407916], Neobenedenia melleni [http://www.ncbi.nlm.nih.gov/pubmed/7707197][http://www.ncbi.nlm.nih.gov/pubmed/1597779], Enterogyrus cichlidarum (associated with overcrowding [http://www.ncbi.nlm.nih.gov/pubmed/7472888] and infection intensity increases with (tilapia) host size[http://www.ncbi.nlm.nih.gov/pubmed/2242958]), Gyrodactylus malalai (ectoparasite)[http://www.ncbi.nlm.nih.gov/pubmed/22807048], Gyrodactylus ergensi [http://www.ncbi.nlm.nih.gov/pubmed/19838735], Scutogyrus longicornis [http://www.ncbi.nlm.nih.gov/pubmed/25054495] and of the genus Cichlidogyrus [http://www.ncbi.nlm.nih.gov/pubmed/22436463], such as Cichlidogyrus tilapiae, Cichlidogyrus sclerosus [http://www.ncbi.nlm.nih.gov/pubmed/21791155], Cichlidogyrus dossoui [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Cichlidogyrus halli [[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Cichlidogyrus thurstonae [http://www.ncbi.nlm.nih.gov/pubmed/14707506], Cichlidogyrus berradae, Cichlidogyrus revesati, Cichlidogyrus legendrei and Cichlidogyrus lemoallei.[http://www.ncbi.nlm.nih.gov/pubmed/14535345] The highest parasite number was recorded in reservoir-dwelling, and lowest in stream-dwelling tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21791155] Gyrodactylus niloticus infection may particularly become lethal (in tilapia) when followed by exposure to the bacterial pathogen Streptococcus iniae.[http://www.ncbi.nlm.nih.gov/pubmed/17394525] Low stocking density and low water temperature may result in lower monogenea parasitism rate.[http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Acanthocephala (thorny-headed worms), such as Acanthogyrus (Acanthosentis) tilapiae.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] and Polyacanthorhynchus kenyensis [http://www.ncbi.nlm.nih.gov/pubmed/12015820] In Kenya, infection rates in wild Tilapia zillii ranged from 4.1 to 77.7%.[http://www.ncbi.nlm.nih.gov/pubmed/9332980] &lt;br /&gt;
* Ciliatea (&amp;#039;small flagella&amp;#039;), such as Epistylis sp.[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Paratrichodina africana [http://www.ncbi.nlm.nih.gov/pubmed/23731856], Trichodina compacta [http://www.ncbi.nlm.nih.gov/pubmed/21755153], Trichodina magna [[http://www.ncbi.nlm.nih.gov/pubmed/25054495] and Ichthyophthirius multifiliis.[http://www.ncbi.nlm.nih.gov/pubmed/22311586] Ichthyophthirius multifiliis load increases susceptibility and mortality of tilapia to Streptococcus iniae (Gram-positive bacteria) infection.[http://www.ncbi.nlm.nih.gov/pubmed/19750806] &lt;br /&gt;
* Dinophyceae (Dinoflagellata; &amp;quot;red tide&amp;quot;), such as Piscinoodinium pillulare.[http://www.ncbi.nlm.nih.gov/pubmed/25054495] (a dominant parasite in Brasilian tilapia ponds)[http://www.ncbi.nlm.nih.gov/pubmed/21755153] &lt;br /&gt;
* Myxosporea, such as Henneguya suprabranchiae [http://www.ncbi.nlm.nih.gov/pubmed/18516619], Myxobolus heterosporus [http://www.ncbi.nlm.nih.gov/pubmed/15819436] (induces total destruction of the intestine structure [http://www.ncbi.nlm.nih.gov/pubmed/12911060]), Myxobolus nounensis [http://www.ncbi.nlm.nih.gov/pubmed/11031757], Myxobolus dossoui (host size effect), Myxobolus zillii (seasonal pattern) [http://www.ncbi.nlm.nih.gov/pubmed/11383569], Myxobolus microcapsularis [http://www.int-res.com/articles/dao/44/d044p217.pdf], Myxobolus agolus, Myxobolus brachysporus, Myxobolus clarii, Myxobolus cichlidarum, Myxobolus tilapiae and Myxobolus camerounensis.[http://www.ncbi.nlm.nih.gov/pubmed/19069850]&lt;br /&gt;
* Conoidasida (unicellular), such as Goussia cichlidarum.[http://www.ncbi.nlm.nih.gov/pubmed/17885764]&lt;br /&gt;
* Phyllopharyngea (single-cell parasites), such as Chilodonella hexasticha.[http://www.ncbi.nlm.nih.gov/pubmed/22902259]&lt;br /&gt;
* Trhypochthoniellus longisetus longisetus, a mite.[http://www.ncbi.nlm.nih.gov/pubmed/21553570]&lt;br /&gt;
* Lamproglena sp. (Lernaeidae; small crustaceans) [http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Pentasomida (tongue worms; tiny crustaceans), such as Leiperia cincinnalis [http://www.ncbi.nlm.nih.gov/pubmed/9809320], Subtriquetra wedli [http://www.ncbi.nlm.nih.gov/pubmed/10192834] and Subtriquetra riley.[http://www.ncbi.nlm.nih.gov/pubmed/9809320]&lt;br /&gt;
* Anodontites trapesialis (the larval stage of this mussel).[http://www.ncbi.nlm.nih.gov/pubmed/12048579]&lt;br /&gt;
&lt;br /&gt;
No parasite vaccines exist.[http://www.ncbi.nlm.nih.gov/pubmed/15757476] Tilapia are relatively resistant to parasitic infections.[http://www.ncbi.nlm.nih.gov/pubmed/18537032] The prevalence of heterophyid encysted metacercariae infection decreases as fish size increases.[http://www.ncbi.nlm.nih.gov/pubmed/20644958] When dissolved oxygen, temperature, pH, nitrite and ammonia are within normal rate parasites may cause little harm to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19500461] Parasitic infection by Acanthogyrus tilapiae provokes an aggressive host response.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] Tilapia may produce an induced humoral immune response against Cichlidogyrus sp.[http://www.ncbi.nlm.nih.gov/pubmed/18564741] Oreochromis mossambicus initiated a strong immune protection by direct exposure with even a small number of parasites.[http://www.ncbi.nlm.nih.gov/pubmed/21739314] Gyrodactylus numbers fluctuate independently of temperature. In anticipation of immune defenses reaching the fish surface through mucus, Gyrodactylids (Monogenea) worms progressively move away from fins with high mucus cell density to those with low density.[http://www.ncbi.nlm.nih.gov/pubmed/21840127] Tilapia may also develop resistance to Neobenedenia sp. (Monogenea) infection.[http://www.ncbi.nlm.nih.gov/pubmed/21381523]&lt;br /&gt;
&lt;br /&gt;
Freshwater mollusks in tilapia ponds, such as Melania tuberculata, Melanoides turricula, Pomacea flagellata, Haitia cubensis and Anodontiles luteola, may host various parasites that are harmful to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21250483] The Biomphalaria sp. snails are often found in tilapia ponds (fresh water reservoirs are their natural habitat[http://www.ncbi.nlm.nih.gov/pubmed/11100444]) and are intermediates for Schistosoma mansoni.[http://www.scielo.br/scielo.php?script=sci_arttext&amp;amp;pid=S0074-02762001000900008&amp;amp;lng=en&amp;amp;nrm=iso&amp;amp;tlng=en] Biomphalaria cf. havanensis is an intermediate host for Diplostomum ompactum, causing higher levels of infections in cultured tilapia than wild tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19452167] The snail Bulinus truncatus is an intermediate host for Clinostomum tilapiae, Euclinostomum heterostomum, Bolbophorus levantinus and Neascus-type metacercariae. The snail Melanoides tuberculata is an intermediate host for Centrocestus sp. and Haplorchis sp. The snail Melanopsis costata is an intermediate host for Pygidiopsis genata, Phagicola longa.[http://www.ncbi.nlm.nih.gov/pubmed/12911062] The snail Thiara granifera is commonly infected with Haplorchis pumilio.[http://www.ncbi.nlm.nih.gov/pubmed/12015833] Dogs, cats and pigs may also be intermediate hosts for metacercariae.[http://www.ncbi.nlm.nih.gov/pubmed/18564743]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Algae_for_Tilapia&amp;diff=4464</id>
		<title>Algae for Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Algae_for_Tilapia&amp;diff=4464"/>
		<updated>2014-12-31T04:36:18Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling, mgl/L --&amp;gt; mg/L ?&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* Plankton are organisms (animal or plant) that live in the water and cannot swim against a current; zooplankton and phytoplankton.&lt;br /&gt;
* Algae includes brown and green algae (planktonic or attached; single-celled or multicellular), and diatoms (planktonic, attached or motile; single-celled or colonial). &lt;br /&gt;
* Blue-green algae (planktonic or attached; single-celled or colonial) are bacteria with photosynthetic capacity; cyanobacteria (cyano = blue).&lt;br /&gt;
&lt;br /&gt;
==Feeding==&lt;br /&gt;
[[Image:Ceriodaphnia_reticulata.jpg|thumb|right| Ceriodaphnia [http://www.micromagus.net/microscopes/pondlife_cladocera.html © micromagus.net]]]&lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Tilapia Tilapia] start feeding shortly before dawn and feed continually (but most intensively between 12:00 and 18:00[http://cabdirect.org/abstracts/20013100658.html;jsessionid=1F937711ACC14AC20505C4692DBC7CB3]) until about dusk. They do not feed during the night.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-7998.1973.tb07513.x/abstract][http://link.springer.com/chapter/10.1007/978-94-009-7281-0_13#page-1] Tilapia (Oreochromis niloticus) may effectively control algal blooms in eutrophic waters.[http://link.springer.com/article/10.1007/s10750-006-0023-5#page-1] Planktonic plants and &lt;br /&gt;
animals make up the bulk of the diet under natural conditions.[http://www.nativefishlab.net/library/textpdf/17926.pdf] Zooplankton (animal) does not exceed 1.5% of total stomach contents [http://www.cabdirect.org/abstracts/20023142580.html], with predation pressure being the greatest on Ceriodaphnia reticulata (small water fleas that feed on small algae)   [http://link.springer.com/article/10.1007/BF00016658#page-1], due to low escape probabilities for (Cerio)daphnia compared to copepods (small crustaceans).[http://www.nrcresearchpress.com/doi/abs/10.1139/f78-215#.VF5gm_mG_QM]&lt;br /&gt;
Phytoplankton (plant plankton) may contribute over 30% by volume of stomach contents of Oreochromis niloticus.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Small Tilapia rendalli consumed more diatoms than larger individuals.[http://aquaticcommons.org/9103/] Oreochromis niloticus feeds mainly on zooplankton until 5 cm long, and mainly on phytoplankton as it grows older. In small fish (&amp;lt;75 g.) Bacillariophyceae are the main phytoplankter, and in larger fish Chlorophyceae are the main phytoplankter.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/500.pdf] Tilapia (Sarotherodon melanotheron) shift from visually feeding on zooplankton when juveniles to mostly filter feeding on phytoplankton when adults [http://www.sciencedirect.com/science/article/pii/S0144860908000666], consuming less Microcystis and Scenedesmus as they grow bigger.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2003.00917.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Blue-green algae (colonies of photosynthetic bacteria; cyanobacteria) are common components of the Tilapia diet. In the stomach of Tilapia nilotica the cells of blue-green algae are lysed by high concentrations of acid (pH 1.4–1.9). After lysis, cell contents are digested in the intestine (by pepsinogen, a pancreatic α-amylase, trypsin, chymotrypsin and esterase activity). Acid is secreted in relation to feeding. Acid is not secreted by stressed fish.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-7998.1973.tb07514.x/abstract] Filtration rates of algae increases linearly as water temperature increases from 17°C to 32°C.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2003.00830.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
The main species of algae found in Oreochromis niloticus stomach belonged to Cyanophyta (2nd most ammonium tolerant[http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae]), Chlorophyta (most ammonium tolerant[http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae]), Bacillariophyta and Euglenophyta.[http://www.cabdirect.org/abstracts/20023142580.html] The most frequent species represented in Oreochromis niloticus stomach are Anabaena sp., Merismopedia eleganse, Microcystis aeruginose, Nodularia harveyana and Oscillatoria sp. (Cyanophyta), Cerasterias sp., Chlorella spp., Crucigenia sp., Pediastrum spp., Scenedesmus spp. and Tetraedron sp. (Chlorophyta), Amphora ovalis, Cocconeis placentula, Cymatopleura solea, Cymbella cistula, Gyrosigma attenuatum, Melosira granulata, Navicula spp., Nitzschia spp., Pinnularia spp., Serurella sp. and Synedra sp. (Bacillariophyta) and Euglena and Phacus spp. (Euglenophyta).[http://www.cabdirect.org/abstracts/20023142580.html] The most frequent genera represented in fish (Oreochromis niloticus) stomach in all sizes were Anabaena, Anabaenopsis, Coleospharium, Merismopedia, Microcystis, Nodularia, Oscillatoria, Spirulina (Cyanobacteria), Actinostrum, Chlorella, Closterium, Coelastrum, Eudrina, Pandorina, Pediastrum, Scenedesmus, Shereoderia, Staurastrum (Chlorophyceae), Amphora, Cocconeis, Cymatopleura, Cymbella, Gyrosigma, Melosira, Navicula, Pinnularia, Synedra (Bacillariophyceae) and Euglena and Phacus (Euglenophyceae).[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/500.pdf] Botryococcus sp. (green algae)[http://link.springer.com/article/10.1007/BF00008157#page-1][http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1987.tb05767.x/abstract] and Melosira (a diatom; Bacillariophyceae)[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1993.tb00396.x/abstract] may also be major food items in the diet of Oreochromis niloticus.&lt;br /&gt;
&lt;br /&gt;
==Selective feeding==&lt;br /&gt;
[[Image:Uroglenopsis.jpg|thumb|right| Uroglenopsis [http://cfb.unh.edu/phycokey/Choices/Chrysophyceae/colonial_chrysophyceae/flagellated/UROGLENOPSIS/Uroglenopsis_Image_page.html © John DuFresene]]]&lt;br /&gt;
[[Image:Spirulina_platensis.jpg|thumb|left| Spirulina platensis [http://mcc.nies.go.jp/images/100images/nies-0046.jpg © http://mcc.nies.go.jp/]]]&lt;br /&gt;
[[Image:Ceratium.jpg|thumb|right| Ceratium [http://protist.i.hosei.ac.jp/pdb/images/Mastigophora/Ceratium/furca_7.html © Y. Tsukii]]]&lt;br /&gt;
&lt;br /&gt;
Tilapia feed selectively on large algae, mainly cyanobacteria and diatoms [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2427.2005.01407.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] and select more for diatoms than for the blue-green algae (cyanobacteria).[http://oceandocs.net/bitstream/1834/1160/1/9290640789-P93103.pdf]&lt;br /&gt;
Oreochromis niloticus may select only Cyanophyta and Euglenophyta (unicellular flagellate protozoa), with occasional Bacillariophyta (diatoms).[http://www.cabdirect.org/abstracts/20013148595.html;jsessionid=0F8AC1DEC89977E92FA074A1E5D3E898] &lt;br /&gt;
Oreochromis niloticus may select Cyanophyta and sometimes select Bacillariophyta and Euglenophyta.[http://www.cabdirect.org/abstracts/20023142580.html] &lt;br /&gt;
Blue tilapias (4.3 to 18.7 cm long) selectively feed on particles larger than 25 μm.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1984)113%3C397%3APAPCIO%3E2.0.CO%3B2#.VCvJePl_s10] &lt;br /&gt;
Nile tilapia is particularly effective in filtering the larger particle size taxa.[http://www.sciencedirect.com/science/article/pii/S0044848602001333] &lt;br /&gt;
Larger phytoplankton (plant plankton) are being filtered proportionally more than the smaller phytoplankton, and cyanobacteria (in 100% of fish[http://www.sciencedirect.com/science/article/pii/0044848678900157]) more than green algae (Scenedesmus, Ankistrodesmus, Tetraedron).[http://www.sciencedirect.com/science/article/pii/S0044848602006142] &lt;br /&gt;
Tilapia aurea (Blue tilapia) favour Uroglenopsis sp. (cells forming hollow spherical colonies, &amp;gt; 400 μm diameter), Ceratium sp.(dinoflagellate (the least ammonium tolerant [http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae]), 310 μm long, 58 μm wide; optimum salinity 10 ppt[http://link.springer.com/article/10.1007/BF00346767#page-1]) and Keratella sp. over (small-sized algae) Rhodomonas sp., Chrysochromulina sp., Chlamydomonas sp., Cyclotella sp. and (zooplankter) Diaptomus sp.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1984)113%3C397%3APAPCIO%3E2.0.CO%3B2#.VCvJePl_s10] Oreochromis niloticus juveniles prefer Elodea canadensis over Potamogeton pectinatus and Spirodela polyrhiza. Myriophyllum spicatum was the least preferred.[http://agris.fao.org/agris-search/search.do?recordID=CZ2005000021]&lt;br /&gt;
&lt;br /&gt;
Diatoms are microscopic unicellular algae, all consisting of the same basic parts; nucleus, cytoplasm, plasma membrane, and the cell wall.[http://www.modernmicroscopy.com/main.asp?article=89&amp;amp;print=true&amp;amp;pix=true] Oreochromis niloticus prefer to prey on the large size diatoms, such as Melosira granulata, Synedra ulna, Cyclotella ocellata and Cyclotella operculata.[http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] &lt;br /&gt;
Astrionella sp. and Rhopalodia vermicularis (bacillariophytes) and Peridinium sp. (dinophyte) were selected by both wild and pond-cultured Oreochromis niloticus. Lake fish particularly selected Astrionella sp., Aulacoseira (Melosira) nyassensis, Navicula sp. (which grow on sediment[http://www.scribd.com/doc/41415598/The-Epipelic-Diatom-Community-at-the-Downstream-Rivers-of-Torong-Galeh-and-Legi-Semarang-Indonesia]) and Rhopalodia vermicularis (bacillariophytes). Pond fish particularly selected Ankistrodesmus falcatus (chlorophyte), Lyngbya circumcreta (cyanophyte) and Nitzschia acicularis.[http://www.tandfonline.com/doi/abs/10.2989/16085914.2013.784698#.VFu___mG_QM] Oreochromis niloticus may prefer Spirulina laxisma and Nitzschia accicularis the most. The least preferred food items may be Lyngbya circumcreta, Microcystis aeruginosa and Pediastrum simplex.[http://www.oceandocs.org/handle/1834/1160]&lt;br /&gt;
&lt;br /&gt;
==Algal growth==&lt;br /&gt;
The favourable pH range for plankton growth ranges between 6.5 and 7.5. Acidic conditions favor chlorophytes (green algae) while alkaline condition fosters nitrogen-fixing cyanobacteria.[http://books.google.nl/books?id=u-NNxcC8Fn0C&amp;amp;dq=%22Royal+Academy+of+Overseas+Sciences%22&amp;amp;ie=ISO-8859-1&amp;amp;source=gbs_gdata&amp;amp;redir_esc=y] High salinity favors green algae (plus Spirulina) while low salinity fosters cyanobacteria. In phytoplankton-based recirculating systems, algae may rather be limited by dissolved organic carbon availability rather than by nitrogen or phosphorus.[http://www.sciencedirect.com/science/article/pii/S0144860908000666] Chlorella vulgaris has higher lipid contents than Scenedesmus abundans and Monoraphidium minitum, which was also reflected in their predator, Brachionus calyciflorus. [http://www.sciencedirect.com/science/article/pii/S0044848699000137] Organic matter of manure (eg chicken manure [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract]) hardly contributes directly to fish growth; only indirectly through carbon incorporated in algae.[http://www.sciencedirect.com/science/article/pii/0044848690901154] Most fertilized fish ponds with dirty green water have more than 242 mg phytoplankton biomass per L water.[http://ppmq.ars.usda.gov/research/publications/publications.htm?seq_no_115=148778] Diatoms are less dependent on trace elements than other algae.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
&lt;br /&gt;
==Brachionus plicatilis==&lt;br /&gt;
[[Image:Brachionus_plicatilis.jpg|thumb|left| Brachionus plicatilis ]]&lt;br /&gt;
[[Image:Akashiwo_sanguinea.jpg|thumb|right| Akashiwo sanguinea [http://www.serc.si.edu/labs/phytoplankton/guide/addtl_collections/Cape%20Cod/GymsanCC.aspx © SERC]]]&lt;br /&gt;
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Brachionus plicatilis is an important food source for larval fish. Brachionus plicatilis is also the most successful species of zooplankton used to control unicellular algal contaminants in Spirulina mass cultures even under conditions adverse to the growth of Spirulina (very low bicarbonate).[http://onlinelibrary.wiley.com/doi/10.1002/bit.260300205/abstract]&lt;br /&gt;
Brachionus plicatilis is a rotifer / zooplankton associated with saline, alkaline waters.[http://www.avto.aslo.info/lo/toc/vol_18/issue_1/0053.pdf] Brachionus plicatilis may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] It may be 123 to 292 μm long and 114 to 199 μm wide. Compared to temperature and salinity, strain is the most important factor determining size.[http://www.sciencedirect.com/science/article/pii/0044848684903004] Maximum reproduction occurs between 30 and 34 C. Reproduction rate is similar in 25% and 33% seawater. Brachionus plicatilis may thrive in 0.5 to 30 ppt salinity (brackish) and may be 99 to 281 μm long and may weigh 0.18 μg. In contrast to female Brachionus rubens, fecund female Brachionus plicatilis do not attach to a substrate. [http://link.springer.com/article/10.1007/BF00354834#page-1] Adult Brachionus plicatilis may be 213 to 315 μm long. Optimum salinity may be 18 ppt. The most common feed types are single-celled algae.[http://nsgl.gso.uri.edu/hawau/hawauw91001/hawauw91001_part5.pdf] Brachionus plicatilis may (together with Nitzschia spp) survive in 10 pH, high calcium carbonate and 91 ppt salinity.[http://link.springer.com/article/10.1007/BF00025190#page-1]&lt;br /&gt;
&lt;br /&gt;
Brachionus plicatilis may be mass cultured on Akashiwo sanguinea (Gymnodinium splendens / nelsoni), an unarmored (naked) dinoflagellate. [http://link.springer.com/article/10.1007/BF00354834#page-1] Akashiwo sanguinea is a mixotroph, as it is both photosynthetic and feeds on cyanobacteria and nanociliate populations (tiny  protozoans)[http://link.springer.com/article/10.1007%2FBF00350065], which may be algivores.[http://link.springer.com/article/10.1007/BF00877447#page-1]&lt;br /&gt;
It may form subsurface chlorophyll maximum layers in the water, which is consumed by larval fish.[http://www.aslo.org/lo/toc/vol_23/issue_2/0219.html] This may be in response to a relative nitrate deficiency during the light period.[http://link.springer.com/article/10.1007%2FBF00388169] Dinoflagellates prefer calm water column situations; small-scale turbulence may diminish dinoflagellate growth and cell division, but may also increase cell size.[http://digital.csic.es/handle/10261/40987] Akashiwo sanguinea may thrive in brackish conditions.[http://link.springer.com/article/10.1007/s12040-011-0073-6#page-1] Coastal Akashiwo sanguinea bloom events may be supported by elevated urea.[http://www.sciencedirect.com/science/article/pii/S156898830800108X]&lt;br /&gt;
&lt;br /&gt;
==Keratella==&lt;br /&gt;
[[Image:Keratella.jpg|thumb|left| Keratella [http://www.plingfactory.de/Science/Atlas/KennkartenTiere/Rotifers/01RotEng/source/Keratella%20quadrata.html © Michael Plewka]]]&lt;br /&gt;
&lt;br /&gt;
Young Oreochromis niloticus mainly feed on zooplankton until ~5 cm long.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/500.pdf] &lt;br /&gt;
Keratella sp. are relatively small (~100 μm long) &amp;#039;wheel animals&amp;#039; / rotifers / zooplankton. Growth may be optimal at 24°C temperature and 20 ppt salinity. Fecundity may be optimal at 0 ppt salinity.[http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=SSHGB2_2013_v25n5_1205] &lt;br /&gt;
&lt;br /&gt;
Keratella cochlearis may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Keratella cochlearis and Keratella quadrata are euryhaline and perennial (not seasonal).[https://bora.uib.no/handle/1956/2511]&lt;br /&gt;
Keratella cochlearis, Keratella americana and Keratella tropica are considered euryhaline (able to adapt to a wide range of salinities). Keratella hispida may thrive on 30 ppt.[http://link.springer.com/article/10.1007%2FBF00006515#page-1] &lt;br /&gt;
Keratella tropicana may occur in shallow waters at 43 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf]&lt;br /&gt;
&lt;br /&gt;
==Peridinium cinctum==&lt;br /&gt;
[[Image:Peridinium_cinctum.jpg|thumb|right| Peridinium cinctum [http://www.photomacrography.net/forum/viewtopic.php?p=99321&amp;amp;sid=7c740085cef2cf3d31b31c57693122ad © Jacek]]]&lt;br /&gt;
&lt;br /&gt;
Peridinium species are large phytoplankton (30-70 μm diameter armoured dinoflagellates) that less efficiently take up nutrients than smaller species. At their advantage is the capacity to modify their position in the water column (in accordance with light and nutrient availability), that they are (because of their large size) inedible by most zooplankton, and their extremely low phosphorus requirements (C:P molar ratio is 460:1).[http://link.springer.com/article/10.1007/s10750-012-1145-6#page-1] They may produce 2 to 3 times more organic matter than other alga.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=GkoJTvXNuggC&amp;amp;oi=fnd&amp;amp;pg=PA167&amp;amp;dq=Peridinium+cinctum+salinity&amp;amp;ots=1XAXxWCTsT&amp;amp;sig=nbgtovZiLf9cFuxG03EkuQKeokc#v=onepage&amp;amp;q=Peridinium%20cinctum%20salinity&amp;amp;f=false]&lt;br /&gt;
&lt;br /&gt;
Tilapia (galilaea) favourably consume Peridinium cinctum.[http://www.sciencedirect.com/science/article/pii/0044848676900478] Feeding rates of Tilapia galilaea and Tilapia aurea reach maximum values for zooplankton (animal plankton) and Peridinium cinctum.[http://www.nrcresearchpress.com/doi/abs/10.1139/f88-082#.VCwiZfl_s10] Fingerlings fed experimentally on chlorophytes had lower growth rates compared with mosquito larvae, zooplankton and fresh Peridinium.[http://www.sciencedirect.com/science/article/pii/0044848680900393] Growth rates of age-1 Tilapia galilaea on Peridinium may be 1–2% mean weight per day.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2427.1979.tb01483.x/abstract] &lt;br /&gt;
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When phosphorus is low, Peridinium has an advantage over other alga.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=GkoJTvXNuggC&amp;amp;oi=fnd&amp;amp;pg=PA167&amp;amp;dq=Peridinium+cinctum+salinity&amp;amp;ots=1XAXxWCTsT&amp;amp;sig=nbgtovZiLf9cFuxG03EkuQKeokc#v=onepage&amp;amp;q=Peridinium%20cinctum%20salinity&amp;amp;f=false] Peridinium aciculiferum may particularly produce toxins when deprived of phosphorus and in stationary phase.[https://lup.lub.lu.se/search/publication/146608] Blooms of Peridinium gatunense (biomass &amp;gt; 100 g / m2) may change the color of the water to coffee brown in distinct patches and are associated with excessive oxygen super-saturation, and high phosphorus (&amp;gt; 0.05 mg/L) and nitrogen (&amp;gt; 1 mg/L).[http://link.springer.com/chapter/10.1007/978-94-017-8944-8_11#page-1] Peridinium gatunense and Microcystis sp. are highly competitive.[http://www.ncbi.nlm.nih.gov/pubmed/12401172] Peridinium ponticum may thrive in 17 ppt salinity.[http://www.sciencedirect.com/science/article/pii/S0025322702003481] Peridinium cinctum is common below 20 ppt salinity.[http://link.springer.com/article/10.1007/BF00025173#page-1]&lt;br /&gt;
&lt;br /&gt;
==Cocconeis placentula==&lt;br /&gt;
[[Image:Cocconeis_placentula.jpg|thumb|left| Cocconeis placentula [https://www.flickr.com/photos/microagua/2634071221/ © Proyecto Agua]]]&lt;br /&gt;
[[Image:Cocconeis_placentula_2.jpg|thumb|right| Cocconeis placentula [http://nivalis.jp/kibun/microscope/gallery/cocconeis/cocconeis.html © 珪藻ギャラリートップへ]]]&lt;br /&gt;
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Oreochromis niloticus preferentially selects diatoms over cyanobacteria.[http://oceandocs.net/bitstream/1834/1160/1/9290640789-P93103.pdf]&lt;br /&gt;
&amp;#039;&amp;#039;Cocconeis placentula&amp;#039;&amp;#039; is frequently represented in Oreochromis niloticus stomach.[http://www.cabdirect.org/abstracts/20023142580.html]&lt;br /&gt;
Cocconeis placentula is a diatom that may be 9-68 µm long and 7-32 µm wide [http://westerndiatoms.colorado.edu/taxa/species/cocconeis_placentula], or up to 98 µm long and 40 µm wide.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13160100.html]&lt;br /&gt;
Cocconeis placentula thrives in moderately saline and moderately alkaline, shallow waters.[http://in-africa.org/wp-content/uploads/2012/12/Owen-et-al-1982-Nature-Holocene-L-Turkana1.pdf] &lt;br /&gt;
Cocconeis placentula readily attaches to substrates due to the secretion of adhesive substances.[http://botanika.bf.jcu.cz/thesis/pdf/Mares%20_bc06.pdf]&lt;br /&gt;
Cocconeis placentula is very common in (brackish / freshwater[http://files.figshare.com/1704702/Table_S6.pdf]) eutrophic [http://www.researchgate.net/publication/237052676_The_Epipelic_Diatom_Community_at_the_Downstream_Rivers_of_Torong_Galeh_and_Legi_Semarang_(Indonesia)], benthic habitats, where it (solitary) attaches to rocks, macrophytes and algae. It is tolerant of moderate organic pollution, with var. euglypta being possibly more tolerant.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13160100.html] Cocconeis placentula may thrive in shallow waters at 6-29°C and 6.9-7.7 pH, with 0-2.5 ppt salinity, 3.0-4.1 meq/L alkalinity, 0.0-0.45 mg/L NH&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;, 0.0-0.09 mg/L phosphates.[http://www.imp-du.com/downloadRadovi/Jasprica_Hafner%20-%20diatoms.pdf] Cocconeis placentula may prefer elevated total phosphates.[http://botanika.bf.jcu.cz/thesis/pdf/Mares%20_bc06.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cocconeis placentula var. euglypta&amp;#039;&amp;#039; is a bit smaller, but also attached solitary, and may survive in lakes with 40 ppt salinity [http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] or brackish / freshwater.[http://files.figshare.com/1704702/Table_S6.pdf]&lt;br /&gt;
&lt;br /&gt;
==Cyclotella==&lt;br /&gt;
[[Image:Ciclotella_ocellata.jpg|thumb|right| Cyclotella ocellata [http://www.redorbit.com/images/pic/28990/diatom-species-cyclotella-ocellata-is-the-most-common-planktonic-diatom-in-lake/ © RedOrbit]]]&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus prefer to prey on the large size diatoms, such as &amp;#039;&amp;#039;Cyclotella ocellata&amp;#039;&amp;#039; and Cyclotella operculata, which are Centrophycidae.[http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] &lt;br /&gt;
&amp;#039;&amp;#039;Cyclotella ocellata&amp;#039;&amp;#039; is cylinder-shaped and may have a diameter of 6 to 25 µm [http://www.planktonforum.eu/index.php?id=33&amp;amp;no_cache=1&amp;amp;L=1&amp;amp;tx_pydb_pi1%5Bzellform%5D=&amp;amp;tx_pydb_pi1%5Bdetails%5D=2021&amp;amp;tx_pydb_pi1%5Bstart%5D=60&amp;amp;tx_pydb_pi1%5Bcur%5D=12&amp;amp;cHash=b812acb971a1b23c82036790bfe9a281] up to 40 µm. (incl. &amp;#039;&amp;#039;C. krammeri&amp;#039;&amp;#039; and &amp;#039;&amp;#039;C. rossii&amp;#039;&amp;#039;) [http://www.bgbm.org/sites/default/files/documents/cediatom117Knie%2BHuebener.pdf] and may be 6.3-16.5 µm long.[http://westerndiatoms.colorado.edu/taxa/species/cyclotella_rossii] Cyclotella ocellata is sometimes considered to be planktonic (floating in the water)[ftp://ftp.ncdc.noaa.gov/pub/data/paleo/paleolimnology/lakelevels/former_ussr/lake_descriptions/textfiles/doodn.txt] or epiphytic (growing on plants). It may be considered oligosaline (0.5 to 5 ppt salinity) [http://books.google.nl/books?id=-1j-4Gx_WRoC&amp;amp;dq=Cyclotella+ocellata+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]p37), with a brackish-freshwater salinity optimum. [http://books.google.nl/books?id=SpuPKw7zZGAC&amp;amp;dq=Cyclotella+ocellata+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]p194) Cyclotella ocellata may dominate in cold waters with low salinity and very little nutrients [https://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=2&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CC0QFjAB&amp;amp;url=http%3A%2F%2Ffegi.ru%2Felibrary%2Felibrary%2Fdoc_download%2F261-morphogenesis-in-cyclotella-ocellata-complex-from-lake-elgygytgyn-chukchi-peninsula-during-the-pl&amp;amp;ei=zTprVPf2Ks7-sATFh4DoDg&amp;amp;usg=AFQjCNHOgJ8dtBLbC-_R5iDc_GRS7rDKjA&amp;amp;sig2=ovEJkM3_4hpvi9x_YjmQuA], but may also thrive in eutrophic waters [http://onlinelibrary.wiley.com/doi/10.1002/iroh.199900041/abstract], at high salinity and turbidity (in shallow waters) [http://www.schweizerbart.de/papers/algol_stud/detail/123/74435/Phytoplankton_seasonality_of_a_shallow_turbid_lake] and at higher temperatures (in diluted estuaries).[https://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=1&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CCYQFjAA&amp;amp;url=http%3A%2F%2Fwscholars.com%2Findex.php%2Fajhe%2Farticle%2Fview%2F80&amp;amp;ei=b1lrVOLmJZPSaJmQgvAI&amp;amp;usg=AFQjCNF6ywGC-0FvelqAu1dLC4vaTylVYQ&amp;amp;sig2=PBdO0SJqZSB84lqXKvuTMA&amp;amp;bvm=bv.79908130,d.d2s] Alkalinity range is 80 to 190 CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; mg/L.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cyclotella operculata&amp;#039;&amp;#039; (5-43 µm diameter, &amp;lt;3 µm depth [http://craticula.ncl.ac.uk/EADiatomKey/html/taxon11.html]) is considered planktonic [ftp://ftp.ncdc.noaa.gov/pub/data/paleo/paleolimnology/lakelevels/former_ussr/lake_descriptions/textfiles/rudush.txt] and may thrive at 7.9 to 8.4 pH (Cl 64 mg/L ; NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; 0.28 mg/L ; P 0.23 mg/L).[http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1] Cyclotella in general thrive in the oligo-mesotrophic range (&amp;#039;deficient&amp;#039; to moderate levels of dissolved nutrients, and high DO) [http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf], but also Cyclotella operculata may dominate in eutrophic (soft and hard) waters, at 27-33°C, 40-67 mg/L alkalinity, 0.5-2.5 mg/L phosphates and 0.2-0.8 mg/L nitrates.[https://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=1&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CCgQFjAA&amp;amp;url=http%3A%2F%2Fwww.scirp.org%2Fjournal%2FPaperDownload.aspx%3FpaperID%3D4797&amp;amp;ei=k2VrVNjBJdLgapjogPAN&amp;amp;usg=AFQjCNGYT8Zpy2bMf8WRU5xXznzJY69JNw&amp;amp;sig2=2UTCg64Xwe6Cg2vWmpBPmQ&amp;amp;bvm=bv.79908130,d.d2s]&lt;br /&gt;
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&amp;#039;&amp;#039;Cyclotella meneghiniana&amp;#039;&amp;#039; (15 µm diameter; benthic[http://www.diatomloir.eu/Diatodouces/araphid.html]) is favoured by high light penetration [https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] and most abundant at high nitrate (4.6 mg/L). (ph 7.7 to 8.0)[http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1] Cyclotella meneghiniana may thrive in shallow water (&amp;lt;50 cm) at 908-1100 mg/L Na; 22-33 mg/L K; 7-8 mg/L Ca; 4-6 mg/L Mg; 3-4 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 0.4 mg/L Fe; 45-48 SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 674-830 mg/L Cl and 25.5-26.5 meq/L alkalinity (carbonates plus bicarbonates).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
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&amp;#039;&amp;#039;Cyclotella pseudostellagera&amp;#039;&amp;#039; (8 µm diameter; euryhaline[http://www.diatomloir.eu/Diatodouces/araphid.html]) may thrive at low Cl (7 mg/L) and high P (59 mg/L, as PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;), 023 mg/L nitrate, 0.26 mg/L NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1]&lt;br /&gt;
&lt;br /&gt;
==Cymbella lanceolata==&lt;br /&gt;
[[Image:Cymbella_lanceolata.jpg|thumb|left| Cymbella lanceolata [http://www.photomacrography.net/forum/viewtopic.php?p=97973&amp;amp;sid=a34b38350a76c560037bf71cfbbe06c6 © Chris_M]]]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cymbella lanceolata&amp;#039;&amp;#039; (aka Frustulia lanceolata, Navicula lanceolata) may be 60-220 µm long and 18-32 µm wide, is alkaliphilous (at pH &amp;gt; 7), not motile [http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13220330.html] and is widely distributed (Arctic, China to Brazil).[http://www.algaebase.org/search/species/detail/?species_id=31225] It is a shallow-water species [http://books.google.nl/books?id=ECwlAQAAIAAJ&amp;amp;dq=Cymbella+lanceolata+alkalinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]pE7) and grows solitary or attached (cell-to-cell) in bushy colonies in brackish / freshwater.[http://files.figshare.com/1704702/Table_S6.pdf] Cymbella lanceolata may thrive in shallow waters at 6-29°C and 6.9-7.7 pH, with 0-2.5 ppt salinity, 3.0-4.1 meq/L alkalinity, 0.0-0.45 mg/L ammonium, 0.0-0.09 mg/L phosphates.[http://www.imp-du.com/downloadRadovi/Jasprica_Hafner%20-%20diatoms.pdf]&lt;br /&gt;
&lt;br /&gt;
==Diatoma vulgaris==&lt;br /&gt;
[[Image:Diatoma_vulgare_colony.jpg|thumb|right| Diatoma vulgare [http://www.alamy.com/stock-photo-Diatoma-vulgare-Diatoma-vulgare-with-phase-contrast-MRI-9524969.html © Alamy]]]&lt;br /&gt;
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&amp;#039;&amp;#039;Diatoma vulgaris&amp;#039;&amp;#039; is 15-60 µm long and 8-12 µm wide and may develop in fresh and brackish water. It forms zig-zag colonies attached to the substrate by a mucilage pad. [http://westerndiatoms.colorado.edu/taxa/species/Diatoma_vulgaris] Diatoma vulgaris is associated with Melosira species and may develop optimally in running water.[http://archive.org/stream/ecologyofalgaesy00tryo/ecologyofalgaesy00tryo_djvu.txt] Diatoma elongatum may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Diatoma vulgaris is considered to be mesotrophic (associated with moderate levels of suspended nutrients).[http://www.researchgate.net/publication/237052676_The_Epipelic_Diatom_Community_at_the_Downstream_Rivers_of_Torong_Galeh_and_Legi_Semarang_(Indonesia)] Alkalinity range is 90 to 200 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Diatoma bottnica&amp;#039;&amp;#039; may be 57 µm long and form attached zig-zag colonies in brackish water.[http://files.figshare.com/1704702/Table_S6.pdf]&lt;br /&gt;
&lt;br /&gt;
==Melosira==&lt;br /&gt;
[[Image:Melosira.jpg|thumb|left| Melosira sp. [http://www.savingwater.co.za/tag/melosira/]]]&lt;br /&gt;
[[Image:Aulacoseira_granulata.jpg|thumb|right| Aulacoseira granulata [http://planktonnet.awi.de/index.php?contenttype=image_details&amp;amp;itemid=16791#content © Barbara Meyer]]]&lt;br /&gt;
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Oreochromis niloticus prefer to prey on the large size diatoms, such as Melosira granulata (&amp;#039;&amp;#039;Aulacoseira granulata&amp;#039;&amp;#039;).[http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] One single cell may be 22 to 37 µm long and 3.5 to 5 µm in diameter.[http://nordicmicroalgae.org/taxon/Aulacoseira%20granulata%20var.%20angustissima] Melosira granulata is often found in turbid water.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] Melosira sp. are meroplanktonic, meaning that they are part of plankton only during the larval stage of their life and spend their adult lives in between the plankton on the reef, which they may enter due to wind-induced mixing.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] Though Melosira granulata may also thrive in waters with relatively little nutrients [http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf], Melosira are considered to have high-nutrient requirements and may occur in completely mixed shallow eutrophic waters. Melosira sp in general are characteristic for low salinity and low alkalinity waters.[http://link.springer.com/chapter/10.1007/978-94-009-3095-7_3#page-1] Melosira granulata may thrive at 7 pH, 110 mg/L bicarbonate alkalinity and 260 mg/L hardness [http://www.researchgate.net/publication/256841993_OCCURRENCE_OF_PHYTOPLANKTON_IN_THE_WATER_BODIES_OF_MIRAJ_TAHASIL_OF_MAHARASHTRA/file/60b7d523e97e84f9aa.pdf] / at 7 to 8 pH [http://www.tandfonline.com/doi/abs/10.1080/00288330.1980.9515855#.VFkfo_mG_QM] / 7.2 to 8.5 pH (total bicarbonate alkalinity: 80 to 170 mg/L) [http://www.moef.nic.in/sites/default/files/nlcp/Indian%20Case%20Studies/Q-4.pdf] / 7.9 to 8.4 pH (Cl 64 mg/L ; NH&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; 0.28 mg/L ; P 0.23 mg/L) [http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1] / at 8 to 9 pH.[http://www.jstor.org/discover/10.2307/1662?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21105124407973] Melosira granulata is associated with freshwater lakes [http://books.google.nl/books?id=bz6UWRbwzHwC&amp;amp;dq=Diatoms+shallow+saline+alkaline+lakes&amp;amp;lr=&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s], but Melosira granulata may also be considered euryhaline (may thrive in a wide range of salinities).[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Melosira+granulata+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s][http://link.springer.com/article/10.1007/BF00025173#page-1] Melosira granulata is more abundant at higher temperatures. [http://www.sciencedirect.com/science/article/pii/S0272771409004636] Dominance of Melosira granulata requires [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae high levels of silicon] and high loadings of phosphorus and nutrient fluxes.[http://www.tandfonline.com/doi/abs/10.1080/0269249X.2003.9705580#.VIfvDjGG810] The alkalinity range may be 30 to 165 mg/L CaCo&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] Melosira granulata is less seasonal than offshore populations of Melosira victoriae and Melosira nyassensis.[http://link.springer.com/article/10.1007/BF00027237#page-1] &lt;br /&gt;
&lt;br /&gt;
Melosira / Aulacoseira (and cyclotella) species are Centrophycidae; centric diatoms as opposed to the other class: the pennate genera. The principal limiting resources of Melosira are light and phosphorus (and silica).[http://link.springer.com/chapter/10.1007/978-3-642-84077-7_20#page-1] &amp;#039;&amp;#039;Melosira distans&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Melosira ambigua&amp;#039;&amp;#039; need much light and have low phosphorus requirements and &amp;#039;&amp;#039;Melosira nyassensis&amp;#039;&amp;#039; needs the least light and the most phosphorus. Melosira granulata (which may adjust to light availability) and &amp;#039;&amp;#039;Melosira agassizii&amp;#039;&amp;#039; are intermediate.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] &amp;#039;&amp;#039;Melosira italica v. valida&amp;#039;&amp;#039; develops optimally in running water (reophilous).[http://pleistocenecoalition.com/vanlandingham/VanLandingham_2006.pdf] &amp;#039;&amp;#039;Melosira italica v. Italica&amp;#039;&amp;#039; is perennial and periphytic, associated with terrestrial habitats, but often suspended in the water.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&lt;br /&gt;
==Navicula==&lt;br /&gt;
[[Image:Anomoeoneis_sphaerophora.jpg|thumb|left| Anomoeoneis sphaerophora [http://s252.photobucket.com/user/Glenodin/media/Anomoeoneis-sphaerophora-2.jpg.html © Glenodin]]]&lt;br /&gt;
[[Image:Navicula_radiosa.jpg|thumb|right| Navicula radiosa [http://westerndiatoms.colorado.edu/taxa/species_image/navicula_radiosa/sem_fragment/4/4 © DUS]]]&lt;br /&gt;
&lt;br /&gt;
Navicula species may be preferentially selected by Oreochromis niloticus.[http://oceandocs.net/bitstream/1834/1160/1/9290640789-P93103.pdf][http://www.tandfonline.com/doi/abs/10.2989/16085914.2013.784698#.VGNeivmG811]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula radiosa&amp;#039;&amp;#039; (aka Schizonema radiosum) may be 40-120 μm long and 8-12 μm wide [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/navicula-radiosa-kutzing-1844], or 52-105 µm long and 8.8-11.2 µm wide.[http://westerndiatoms.colorado.edu/taxa/species/navicula_radiosa] It thrives at 21 to 27°C. It is often found in littoral waters and is indifferent of pH (&amp;gt;7 pH) and cationity.[http://content.lib.utah.edu/utils/getfile/collection/etd2/id/2138/filename/580.pdf] Navicula radiosa may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Navicula radiosa may attach to substrates, but not readily.[http://hrcak.srce.hr/file/5634]&lt;br /&gt;
&lt;br /&gt;
Most other navicula are small. Navicula species are considered to be planktonic; floating suspended in the water.[http://www.researchgate.net/publication/237052676_The_Epipelic_Diatom_Community_at_the_Downstream_Rivers_of_Torong_Galeh_and_Legi_Semarang_(Indonesia)]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula radiosa var. radiosa&amp;#039;&amp;#039; is 38-45 µm long, may be periphytic, current indifferent and confined to freshwater.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] &lt;br /&gt;
&amp;#039;&amp;#039;Navicula placentula&amp;#039;&amp;#039; (30-50 μm long and 12-20 μm [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/placoneis-placentula-ehrenberg-mereschkowsky-1903], or 23-28 µm long) may occur suspended in shallow waters.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
Navicula gottlandica var. gottlandica (36-44 µm long, 8-10 µm wide) may thrive in freshwater and brackish water. The temperature tolerance range is 0°-25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] &lt;br /&gt;
&amp;#039;&amp;#039;Navicula atomus, Navicula cari, Navicula cocconeiformis&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Navicula graciloides&amp;#039;&amp;#039; may grow in shallow lakes with 40 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula placentula f. rostrata&amp;#039;&amp;#039; is 37 µm long and 13.5 µm wide.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula arenaria&amp;#039;&amp;#039; may be 30-80 µm long [http://dbmuseblade.colorado.edu/DiatomTwo/dscb_site/species.php?g=Navicula&amp;amp;s=arenaria] and tolerate 8 ppt to 45 ppt salinity.[http://link.springer.com/article/10.1007/BF00395587#page-1] &lt;br /&gt;
&amp;#039;&amp;#039;Navicula halophila&amp;#039;&amp;#039; (aka Craticula halophila f. robusta, aka Navicula cuspidata var. halophila) may be 20-90 (140) µm long and 8-18 µm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/a-g/craticula-halophila-grunow-mann-in-round-crawford-mann-1990] Navicula halophila may grow in lakes with &amp;gt; 10 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;pg=PA203&amp;amp;lpg=PA203&amp;amp;dq=Navicula+halophila+salinity&amp;amp;source=bl&amp;amp;ots=EZrWpQjOfp&amp;amp;sig=fNBKrN4F547R3x4knyc1KTtTgp8&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=QjlqVLapOI3uaqzOgNgN&amp;amp;ved=0CCYQ6AEwAA#v=onepage&amp;amp;q=Navicula%20halophila%20salinity&amp;amp;f=false]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula cryptocephala var. veneta&amp;#039;&amp;#039; (14-18 µm long and 5 µm wide) may occur over a broad range of salt concentrations in running water with pH over 7. It is periphytic or suspended in the (eutrophic) water. Never dominant. Range of tolerance is 0-25°C.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Navicula cryptocephala&amp;#039;&amp;#039; (20-40 μm long [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/navicula-cryptocephala-kutzing-1844], or 21-34 µm long, 5.2-6.5 µm wide[http://westerndiatoms.colorado.edu/taxa/species/navicula_cryptocephala]) may grow at 15 ppt salinity [http://link.springer.com/article/10.1007/BF00396925#page-1], needs pH over 7 and nitrogen in the form of ammonia or amino acids. It is eutrophic and common in both flowing and standing water. Navicula cryptocephala is perennial and may tolerate between 0 and 25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] It is favoured by high light penetration [https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] and is considered eurytopic (the ability to exist in a wide range of chemical environments) and may thrive at 0.15 to 4.6 mg/L nitrate, 7 to 65 mg/L chloride, 0.13 to 0.59 mg/L total phosphorus (as PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;) and 0.00 to 0.28 mg/L NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; [http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1], and in shallow water (&amp;lt;50 cm) at 1100 mg/L Na; 33 mg/L K; 8 mg/L Ca; 4 mg/L Mg; 3 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 45 SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 830 mg/L Cl and 26.5 meq/L alkalinity (carbonates plus bicarbonates).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Anomoeoneis sphaerophora&amp;#039;&amp;#039; (aka &amp;#039;&amp;#039;Navicula sphaerophora&amp;#039;&amp;#039;) may be 40-80 μm long [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/a-g/anomoeoneis-sphaerophora-ehrenberg-pfitzer-1871] or 25-200 µm long and 12-60 µm wide.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13070020.html] It is officially a benthic freshwater specie [http://www.algaebase.org/search/species/detail/?species_id=o2d66a17cc9854d7d&amp;amp;sk=0&amp;amp;from=results], but may be associated with Spirulina platensis dominance (and Nitzschia sigma) in highly alkaline, saline waters [http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] (8.3 - 10.6 pH) [http://books.google.nl/books?id=Rm08AAAAIAAJ&amp;amp;dq=Anomoeoneis+sphaerophora+spirulina+benthic&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]page280. Together with Nitzschia sp. 1 (12% of diatoms present) and Nitzschia sigma (8%) it may predominate (54%) in 18 ppt salinity, 284 mg/L potassium, 54 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 554 meq/L alkalinity, 120 mg/L phosphates and 9.8 pH. Together with Nitzschia sp. 1 (35% of diatoms present) and Nitzschia sigma (13%) it may dominate (43%) in 2 ppt salinity, 53 mg/L potassium, 10.6 mg/L calcium, 32 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 70 meq/L alkalinity, 11.2 mg/L phosphates and 10.3 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Anomoeoneis sphaerophora var. rostrata&amp;#039;&amp;#039; may dominate in &amp;lt;50 cm shallow waters at 9.6 pH and 25.5-130 meq/L (carbonates + bicarbonates) alkalinity (908-4810 mg/L Na; 22-45 mg/L K; 7-8 mg/L Ca; 1-6 mg/L Mg; 0.4 mg/l Fe; 0.7-3 SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 45-68 mg/L SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 674-2680 mg/L Cl).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Anomoeoneis sphaerophora var. sphaerophora&amp;#039;&amp;#039; is benthic and may dominate in 13 to 64 cm shallow fresh and brackish waters at pH 9.7 to 10.1 and 23.1ºC to 34.2ºC ; conductivity &amp;gt; 2870 µS/cm, and may also thrive in waters with lower pH (5.0-10.4) and lower conductivity (318-12070 µS/cm), and at 23.0-36.1°C [http://base.repositorio.unesp.br/bitstream/handle/11449/73904/2-s2.0-84875037147.pdf?sequence=1]&lt;br /&gt;
&lt;br /&gt;
==Nitzschia==&lt;br /&gt;
[[Image:Nitzschia_sp1.jpg|thumb|right| Nitzschia sp. 1 [http://dl.uncw.edu/digilib/biology/protists/taxonomy%20and%20systematics/meps_diatoms/site_05/st5_nitzschia_01.jpg]]]&lt;br /&gt;
[[Image:Nitzschia_accicularis.jpg|thumb|left| Nitzschia accicularis [http://www.serc.si.edu/labs/phytoplankton/guide/diatoms/nitzacic.aspx © SERC]]]&lt;br /&gt;
[[Image:Nitzschia_hungarica.jpg|thumb|right| Nitzschia hungarica [http://www.diatomloir.eu/Site%20Diatom/Saumatdeux.html]]]&lt;br /&gt;
[[Image:Nitzschia_sigma.jpg|thumb|left| Nitzschia sigma [http://www.mikroskopie-forum.de/index.php?topic=15303.0 © Frank Fox]]]&lt;br /&gt;
[[Image:Nitzschia_vermicularis_2.jpg|thumb|right| Nitzschia vermicularis [http://macroclub.ru/gallery/showphoto.php?photo=100656&amp;amp;cat=528 © A. Mikhaltsov]]]&lt;br /&gt;
[[Image:Nitzschia_sigmoidea.jpg|thumb|left| Nitzschia sigmoidea [http://www.diatomloir.eu/Site%20Diatom/Saumatdeux.html]]]&lt;br /&gt;
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Oreochromis niloticus may prefer Spirulina and &amp;#039;&amp;#039;Nitzschia acicularis&amp;#039;&amp;#039; the most.[http://www.oceandocs.org/handle/1834/1160] Nitzschia acicularis (aka synedra acicularis) may be 30-50 µm long.[http://eprints.uni-kiel.de/644/] / 30-100 µm long and 3-4 µm wide [http://westerndiatoms.colorado.edu/taxa/species/nitzschia_acicularis] or 30-150 µm long and 2,2-5 µm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-acicularis-kutzing-smith-1853] Cell volume may be 280 μm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://link.springer.com/article/10.1007/s00442-004-1708-y#page-1] / may vary from 248 μm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; to 800 μm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;[http://eol.org/pages/910724/overview]. Nitzschia acicularis is solitary motile in brackish / freshwater.[http://files.figshare.com/1704702/Table_S6.pdf] Nitzschia acicularis may tolerate high salinity.[http://www.akademiai.com/content/uph511t78463q663/] Alkalinity range is 80 to 220 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] Nitzschia acicularis may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Nitzschia acicularis may thrive in water with 8.3-8.6 pH, 4.5-7.0 DO, 125-309 total alkalinity, 70-220 mg/L Cl, 10-45 mg/L sulphate, 2-4 mg/L phosphate, 6.9-12.6 mg/L Mg, 7.5-10.5 mg/L Ca, 4.1-0.0 mg/L nitrate, 2.5-5.0 mg/L ammonium and 17-18.5ºC[http://www.jeb.co.in/journal_issues/200601_jan06/paper_06.pdf] Nitzschia acicularis may be found simultaneously in its planktonic and attached forms. Most intensive development of Nitzschia acicularis may primarily be in shallow waters in the river delta areas. It is typically found in rivers, ponds, and sometimes streams and bogs, but is very rarely found in lakes.[http://www.plankton.jp/PBE/issue/vol46_1/vol46_1_018.pdf]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia&amp;#039;&amp;#039; are Pennatophycidae (Bacillariophyta [http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf]), which are &amp;#039;&amp;#039;epipelic&amp;#039;&amp;#039; diatoms; benthic (=not free floating) diatoms growing on sediment.[http://www.scribd.com/doc/41415598/The-Epipelic-Diatom-Community-at-the-Downstream-Rivers-of-Torong-Galeh-and-Legi-Semarang-Indonesia] Upper ammonium tolerance of diatoms may be 3600 μM.[http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae] Nitzschia species are not dominant at temperatures below 23°C.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] Many Nitzschia species stop growing at pH values of 8.7 to 9.1, and data suggest that smaller species have a higher upper pH limit for growth than larger species.[http://cat.inist.fr/?aModele=afficheN&amp;amp;cpsidt=15924847] Most Nitzschia may survive in 10 pH.[http://ofmpub.epa.gov/storpubl/storet_wme_pkg.Display_Station?p_station_id=REFRC2&amp;amp;p_org_id=MDEQ_WQ_WQX] Nitzschia are intermediates along the silicon:phosphorus gradient; with relatively balanced silicon and phosphorus requirements.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] Nitzschia species do very [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae poorly in low SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;] waters.[[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia sigma&amp;#039;&amp;#039; may be 388 µm long [http://files.figshare.com/1704702/Table_S6.pdf] 35-1000 µm long [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf] / 30-200 µm long and 4-13 µm wide [http://westerndiatoms.colorado.edu/taxa/species/nitzschia_sigma1], or 40-180 µm long and 4-8 µm wide[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-sigma-kutzing-smith-1853], is periphytic, thrives at pH &amp;gt;7, and may occur over a wide range of salt concentrations. [http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] It is associated with fresh- [http://files.figshare.com/1704702/Table_S6.pdf], saline, alkaline [http://qspace.library.queensu.ca/handle/1974/500], and even hypersaline waters.[http://link.springer.com/chapter/10.1007/978-3-642-70290-7_17#page-1] It may tolerate up to 45 ppt salinity.[http://link.springer.com/article/10.1007/BF00395587#page-1] Nitzschia sigma is capable of free movement [http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.1950.tb07503.x/abstract], benthic and typically found in estuarine (brackish) waters. It has its optimum at 25°C or higher.[http://link.springer.com/article/10.1007/BF00395586#page-1] It is tolerant of pollution. [http://link.springer.com/article/10.1007/PL00012144#page-1] Nitzschia sigma grows well when exposed to 380–450 μM water ammonium concentrations.[http://www.tandfonline.com/doi/abs/10.1080/09670269810001736673#.VGizfvmG810]&lt;br /&gt;
* Together with Nitzschia sp. 1 (35% of diatoms present) and Amonoeoneis sphaerophora (43%) it may grow well (13%) in 2 ppt salinity, 53 mg/L potassium, 10.6 mg/L calcium, 32 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 70 meq/L alkalinity, 11.2 mg/L phosphates and 10.3 pH.&lt;br /&gt;
* Together with Nitzschia sp. 1 (12% of diatoms present) and Amonoeoneis sphaerophora (54%) it may grow well (8%) in 18 ppt salinity, 284 mg/L potassium, 54 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 554 meq/L alkalinity, 120 mg/L phosphates and 9.8 pH.&lt;br /&gt;
* It may dominate (48% of diatoms present) in 5.8 ppt salinity, 69 mg/L potassium, 5.7 mg/L calcium, 34 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 163 meq/L alkalinity, 15.5 mg/L phosphates and at 10.5 pH. &lt;br /&gt;
* It may dominate (45% of diatoms present) in 1.9 ppt salinity, 54 mg/L potassium, 12 mg/L calcium, 28 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 30 meq/L alkalinity, 5.2 mg/L phosphates and at 10.5 pH.&lt;br /&gt;
* It may dominate (41% of diatoms present) in 3.8 ppt salinity, 284 mg/L potassium, 177 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 107 meq/L alkalinity, 9.2 mg/L phosphates and at 9.4 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia sigmoidea&amp;#039;&amp;#039; may attach to substrates [http://hrcak.srce.hr/file/5634], be motile, suspended in brackish water or freshwater and may be 314 µm [http://files.figshare.com/1704702/Table_S6.pdf] 90-500 µm [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf] / 75-250 µm [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf] / 282-350 µm long and 12,5-26 µm wide. It may be periphytic, eutrophic, current indifferent, alkaliphilous and characteristic of the zone where oxidation of biodegradable compounds is complete. It is perennial and tolerates 0 to 25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia sp. 1&amp;#039;&amp;#039; may attach to substrates [http://hrcak.srce.hr/file/5634] and concentrations occur when input water has [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae high Si:P ratios] (warm, wet climate).[http://qspace.library.queensu.ca/handle/1974/500] Nitzschia sp. 1 may be 179 µm long and 6 µm wide.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] Nitzschia sp. 1 (only 85 µm long) is benthonic and only dominant in extremely shallow (saline, alkaline) waters.[http://www.avto.aslo.info/lo/toc/vol_18/issue_1/0053.pdf] Similar to Amonoeoneis sphaerophora and Nitzschia sigma, it may do very well (35% of diatoms present) in 2 ppt salinity, 53 mg/L potassium, 10.6 mg/L calcium, 32 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;,70 meq/L alkalinity, 11.2 mg/L phosphates and 10.3 pH. Equally so, it may also grow well (12% of diatoms present) in 18 ppt salinity, 284 mg/L potassium, 54 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 554 meq/L alkalinity, 120 mg/L phosphates and 9.8 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia hungarica&amp;#039;&amp;#039; (aka Tryblionella hungarica; 35-130 µm long [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-hungarica-grunow-1862]) is benthonic and only dominant in extremely shallow (saline, alkaline) waters.[http://www.avto.aslo.info/lo/toc/vol_18/issue_1/0053.pdf] Nitzschia hungarica is motile, current indifferent, epilythic (growing on sediment), thrives in brackish (/ fresh[http://files.figshare.com/1704702/Table_S6.pdf]) and running water with pH &amp;gt;7.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] It may do well (29% of diatoms present) in 3.7 ppt salinity, 174 mg/L potassium, 8.7 calcium, 1500 mg/L sulphates, 11.8 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 87.4 meq/L alkalinity and 19 mg/L phosphates and 9.0 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia vermicularis&amp;#039;&amp;#039; may be motile in brackish waters and may be 220 µm long and 18 µm wide[http://macroclub.ru/gallery/showphoto.php?photo=100656&amp;amp;cat=528], 75-250 µm [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf], 170 µm [http://files.figshare.com/1704702/Table_S6.pdf] or 140 µm long, and 6 µm wide. It is periphytic, pH indifferent, current indifferent, and thrives in fresh water, tolerating small amounts of salt. It is characteristic of the zone where oxidation of biodegradable compounds is complete.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia palea&amp;#039;&amp;#039; (aka Synedra palea; 12-42 µm long[http://westerndiatoms.colorado.edu/taxa/species/nitzschia_palea], or 15-70 µm long and 2,5-5 µm wide[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-palea-kutzing-smith-1856], or 19-53 µm long, 3.8-5 µm wide) may be the most resistant and most tolerant of all diatoms. It is solitary motile [http://files.figshare.com/1704702/Table_S6.pdf], eurytopic (indifferent of chemical conditions), euryhaline (indifferent of salinity) and eurythermic (indifferent of temperature) and grows in polluted water (but containing moderate dissolved oxygen) forming a brown surface layer on rocks of rapids, as well as in quiet water on shallow silt banks.[http://archive.org/stream/ecologyofalgaesy00tryo/ecologyofalgaesy00tryo_djvu.txt] Nitzschia palea may occur in shallow waters [https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] at 43 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] Domination is reed-associated. Nitzschia palea may dominate in &amp;lt;50 cm shallow waters at 9.6 pH and 25.5-130 meq/L (carbonates + bicarbonates) alkalinity (908-4810 mg/L Na; 22-45 mg/L K; 7-8 mg/L Ca; 1-6 mg/L Mg; 0.4 mg/l Fe; 0.7-3 SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 45-68 mg/L SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 674-2680 mg/L Cl).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
Nitzschia palea may dominate in 13 to 44 cm shallow fresh water at pH 7.0 to 8.5, and at 23 to 32°C (Optimum range: 22.8-29.5°C); conductivity 430-&lt;br /&gt;
2110 µS/cm.[http://base.repositorio.unesp.br/bitstream/handle/11449/73904/2-s2.0-84875037147.pdf?sequence=1]&lt;br /&gt;
Nitzschia palea has its maximum rate of photosynthesis at 33°C and is strongly inhibited at 40°C.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
Nitzschia palea is considered eurytopic (the ability to exist in a wide range of chemical environments) and may thrive at 0.15 to 4.6 mg/L nitrate, 7 to 65 mg/L chloride, 0.13 to 0.59 mg/L total phosphorus (as PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;) and 0.00 to 0.28 mg/L NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;. [http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1]&lt;br /&gt;
Nitzschia palea is eutrophic, pH indifferent, current indifferent and may be suspended in the water or attached to substrates.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia frustulum&amp;#039;&amp;#039; (18 µm long) is an estuarine, benthic diatom [http://onlinelibrary.wiley.com/doi/10.1046/j.1529-8817.2000.99148.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] that is solitary motile [http://files.figshare.com/1704702/Table_S6.pdf] and develops optimally in stagnant waters (limnophilous).[http://www.jstor.org/discover/10.2307/2433656?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21105171827623] Nitzschia frustulum (aka Synedra minutissima / perpusilla / quadrangula) is both benthonic and associated with Spirulina platensis blooms. Nitzschia frustulum is a nitrogen heterotroph and dense populations of Spirulina platensis release organic nitrogen. In the presence of Spirulina platensis (so that the diatom can enter the plankton), Nitzschia frustulum is the dominant diatom in saline lakes with alkalinity 80 &amp;gt; mEq/L, but also survives in freshwater.[http://qspace.library.queensu.ca/handle/1974/500] Nitzschia frustulum may grow in waters with 3 to 100 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] It may be predominant in the range of 3.9 to 4.8 ppt salinity, 164-188 meq/L alkalinity and 10.1-10.4 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia frustulum var. frustulum&amp;#039;&amp;#039; (aka Synedra frustulum) may be 5-60 μm long and 2-4,5 μm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-frustulum-var.-frustulum-kutzing-grunow-in-cleve-grunow-1880]&lt;br /&gt;
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&amp;#039;&amp;#039;Nitzschia americana&amp;#039;&amp;#039; grows optimally at 26 ppt salinity.[http://plankt.oxfordjournals.org/content/8/1/215.abstract] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia archibaldii&amp;#039;&amp;#039; may thrive at 7.1 to 8.2 pH, 0 to 15 mg/L carbonates plus 110 to 170 mg/L bicarbonates alkalinity and 188 to 260 mg/L hardness.[http://www.researchgate.net/publication/256841993_OCCURRENCE_OF_PHYTOPLANKTON_IN_THE_WATER_BODIES_OF_MIRAJ_TAHASIL_OF_MAHARASHTRA/file/60b7d523e97e84f9aa.pdf] It may be 23 µm long and grow in marine / brackish water. It is solitary motile.[http://files.figshare.com/1704702/Table_S6.pdf]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia bolivianais&amp;#039;&amp;#039; is epilithic; growing on rocks.[http://www.bioone.org/doi/abs/10.1635/0097-3157(2007)156%5B123:EDBFCF%5D2.0.CO%3B2]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia closterium&amp;#039;&amp;#039; exhibits optimum photosynthetic activity at 10 ppt [http://link.springer.com/article/10.1007/BF00346767#page-1], but it may tolerate salinity over 35‰ (ppt)[http://m.m.aslo.info/lo/toc/vol_8/issue_2/0263.pdf], even up to 100 ppt.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia denticulata&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Nitzschia dissipata&amp;#039;&amp;#039; (solitary motile[http://files.figshare.com/1704702/Table_S6.pdf], 11.9-72 µm long[http://westerndiatoms.colorado.edu/taxa/species/nitzschia_dissipata]) in particular (and Nitzschia accicularis to a lesser extent) may thrive at 7.2 to 8.5 pH (total bicarbonate alkalinity: 80 to 170 mg/L).[http://www.moef.nic.in/sites/default/files/nlcp/Indian%20Case%20Studies/Q-4.pdf] Nitzschia dissipata is benthic and typically found in estuarine (brackish) waters. It has its optimum at 25°C or higher.[http://link.springer.com/article/10.1007/BF00395586#page-1]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia fonticola&amp;#039;&amp;#039; (10-55 µm long [http://westerndiatoms.colorado.edu/taxa/species/nitzschia_fonticola] or 18-31 µm long) is euryhaline and may thrive in 7 to 50 ppt.[http://www.cambridge.org/nl/academic/subjects/life-sciences/plant-science/diatoms-applications-environmental-and-earth-sciences?format=PB] Unlike most diatoms, Nitzschia fonticola is a nitrogen heterotroph (requiring organic nitrogen).[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia granulata&amp;#039;&amp;#039; may grow in shallow waters at 43 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia intermedia&amp;#039;&amp;#039; is solitary motile, thrives in brackish or freshwater and may be 80 µm [http://files.figshare.com/1704702/Table_S6.pdf] or 132 µm long and 7 µm wide.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&amp;#039;&amp;#039;Nltzschia linearis&amp;#039;&amp;#039; (related to Nitzschia palea) is truly rheobiontic (especially found in running water).[http://archive.org/stream/ecologyofalgaesy00tryo/ecologyofalgaesy00tryo_djvu.txt], 34-228 µm long [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf], 60-150 µm long and 4-6 µm wide[http://westerndiatoms.colorado.edu/taxa/species/nitzschia_linearis], or 106-157 µm long and 5.4-6.5 µm wide, periphytic, eutrophic, and thrives in running freshwater (at 7 pH or above) but able to tolerate small amounts of salt (&amp;lt; 500 mg/L). Tolerates 0 to 30°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia ovalis&amp;#039;&amp;#039; may grow at salinities from 5–40‰ (ppt).[http://link.springer.com/article/10.1007%2FBF00397604#page-1]  &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia vitrea&amp;#039;&amp;#039; may grow in 3 to 100 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
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Some (&amp;#039;&amp;#039;Pseudo&amp;#039;&amp;#039;)Nitzschia species produce domoic acid, a neurotoxin that may cause amnesic shellfish poisoning in humans. Nitzschia pungens [http://www.nrcresearchpress.com/doi/abs/10.1139/f89-156#.VFpLUvmG_QM], Nitzschia pseudodelicatissima [http://www.int-res.com/articles/meps/67/m067p177.pdf]) and particularly Pseudo-nitzschia species in high pH conditions [http://cat.inist.fr/?aModele=afficheN&amp;amp;cpsidt=15924847], such as the cosmopolites Pseudo-nitzschiaseriata delicatissima and Pseudo-nitzschiaseriata pseudodelicatissima, but most notably the cosmopolites Pseudo-nitzschiaseriata australis and Pseudo-nitzschiaseriata multiseries [http://www.sciencedirect.com/science/article/pii/S1568988302000148] may produce domoic acid in the presence of light.[http://www.nrcresearchpress.com/doi/abs/10.1139/f91-137#.VFpO6PmG_QM] Pseudo-Nitzschia may tolerate up to 45 ppt salinity.[http://www.academia.edu/211282/Effects_of_salinity_on_Pseudo-nitzschia_species_Bacillariophyceae_growth_and_distribution]&lt;br /&gt;
&lt;br /&gt;
==Rhopalodia==&lt;br /&gt;
[[Image:Rhopalodia_gibba.jpg|thumb|left| Rhopalodia gibba [http://www.diatomloir.eu/Diatodouces/Canalraphe.html © diatomloir.eu]]]&lt;br /&gt;
[[Image:Rhopalodia_vermicularis.jpg|thumb|right| Rhopalodia vermicularis [http://www.lenaturaliste.net/forum/viewtopic.php?f=90&amp;amp;t=14237&amp;amp;p=77151 © Jean Peynichou]]]&lt;br /&gt;
&lt;br /&gt;
Rhopalodia spp. are littoral diatoms, thriving in brackish waters.[http://link.springer.com/chapter/10.1007/978-3-642-79066-9_15#page-1] Rhopalodia sp are epipelic algae; growing on sediment.[http://www.academia.edu/5264297/QUALITATIVE_AND_QUANTITATIVE_STUDY_OF_EPIPELIC_ALGAE_AND_RELATED_ENIVERNOMENTAL_PARAMETERS_IN_AL-HILLA_RIVER_IRAQ] Rhopalodia is endemic to East Africa [http://books.google.nl/books?id=aCjIB5YRtnkC&amp;amp;dq=Rhopalodia+vermicularis&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]. &lt;br /&gt;
&lt;br /&gt;
Despite their low abundance in pond water, both wild and pond-cultured Oreochromis niloticus preferentially selected &amp;#039;&amp;#039;Rhopalodia vermicularis&amp;#039;&amp;#039;.[http://www.tandfonline.com/doi/abs/10.2989/16085914.2013.784698#.VFu___mG_QM] Rhopalodia vermicularis may be 125 μm long and 14 μm wide. [http://planktonnet.awi.de/index.php?contenttype=image_details&amp;amp;itemid=60625#content] and dominate in shallow waters.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] Rhopalodia vermicularis thrives in moderately saline and moderately alkaline, shallow waters. [http://in-africa.org/wp-content/uploads/2012/12/Owen-et-al-1982-Nature-Holocene-L-Turkana1.pdf]. Rhopalodia vermicularis grows in fresh to hyperalkaline lakes; 7 to 9.5 pH and 10 to 100 meq/L alkalinity (sodium bicarbonate or calcium- plus magnesium bicarbonate), at 21 to 27°C. [http://content.lib.utah.edu/utils/getfile/collection/etd2/id/2138/filename/580.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Rhopalodia gibba&amp;#039;&amp;#039; (aka Navicula gibba) may be 22-300 μm long and 18-30 μm wide [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/rhopalodia-gibba-ehrenberg-muller-1895], 75-205 µm long and 8-11 µm wide.[http://westerndiatoms.colorado.edu/taxa/species/rhopalodia_gibba], grow at pH 10 and low alkalinity; 9.[http://ofmpub.epa.gov/storpubl/storet_wme_pkg.Display_Station?p_station_id=REFRC2&amp;amp;p_org_id=MDEQ_WQ_WQX] Rhopalodia is epiphytic [http://westerndiatoms.colorado.edu/taxa/species/rhopalodia_gibba], periphytic, solitary, and usually occurs in high pH fresh- or brackish water.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13690050.html] Rhopalodia gibba may occur in alkaline freshwater (&amp;lt; 500 mg salt/L), is eutrophic and may tolerate heavy pollution with nitrogen in the form of amino acids or ammonia. It is both periphytic and suspended in water. It occurs in lakes, rivers and streams and is current indifferent.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] Rhopalodia gibba may also grow in 20 to 50 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] Rhopalodia gibba thrives in shallow water, positively correlating with total phosphates.[http://botanika.bf.jcu.cz/thesis/pdf/Mares%20_bc06.pdf] Nitrogen limitation may favor Rhopalodia gibba relative to other diatoms, presumably due to the ability of their cyanobacterial endosymbionts to fix atmospheric nitrogen. As ambient nitrogen levels decrease, the number of endosymbionts increases, but only in the presence of sufficient phosphorus. [http://nau.edu/Centers-Institutes/Ecoss/_Forms/Publications/deyoe-lowe-and-marks-1992/] Together with Synedra ulna, it may grow in 1.2 ppt salinity, 10 mg/L potassium, 56.4 mg/L calcium, 21 mg/L SiO2, 5.7 meq/L alkalinity, 1.9 mg/L phosphates and 8.6 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Rhopalodia gibberula&amp;#039;&amp;#039; (aka Epithemia gibberula / Eunotia gibberula) may be 25-80 µm long and 7-14 µm wide [http://dbmuseblade.colorado.edu/DiatomTwo/dscb_site/species.php?g=Rhopalodia&amp;amp;s=gibberula]), may grow in marine and freshwater [http://www.marbef.org/data/aphia.php?p=taxdetails&amp;amp;id=149568] and may dominate in hypersaline waters.[http://link.springer.com/chapter/10.1007/978-3-642-70290-7_17#page-1] Rhopalodia gibberula may thrive in 3 to 50 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Rhopalodia gracilis&amp;#039;&amp;#039; may dominate in shallow waters.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
&lt;br /&gt;
==Synedra Ulna==&lt;br /&gt;
[[Image:Synedra_ulna.jpg|thumb|right| Synedra ulna [http://www.diatomloir.eu/Diatodouces/diatomun/Synedra%20ulna.jpg © diatomloir.eu]]]&lt;br /&gt;
Oreochromis niloticus prefer to prey on the large size diatoms, such as &amp;#039;&amp;#039;Syndra ulna&amp;#039;&amp;#039; [http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] (aka Fragilaria ulna). It may be 27-600 μm long and 3,5-9 μm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/a-g/fragilaria-ulna-nitzsch-lange-bertalot-1980]&lt;br /&gt;
&amp;#039;&amp;#039;Synedra ulna&amp;#039;&amp;#039; may be considered a benthic freshwater diatom that thrives in shallow estuarine environments close to freshwater sources. [http://books.google.nl/books?id=HrNwdRj1ehoC&amp;amp;dq=Synedra+ulna+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] Synedra ulna readily attaches to substrates by mucilage stalks or pads.[http://hrcak.srce.hr/file/5634] It may form a bushy, attached colony in brackish / freshwater [http://files.figshare.com/1704702/Table_S6.pdf], in shallow, saline, turbid water [http://www.schweizerbart.de/papers/algol_stud/detail/123/74435/Phytoplankton_seasonality_of_a_shallow_turbid_lake], or in shallow, oligotrophic lakes,[http://www.imp-du.com/downloadRadovi/Jasprica_Hafner%20-%20diatoms.pdf].&lt;br /&gt;
Synedra ulna is benthic (rocks, stones, plants) and may also be suspended in the water. It prefers dirty, flowing water with high nitrate, and may be most abundant at 22 to 39°C (optimum 25°C). It may tolerate 5.7-9.0 pH (optimum 6.4-8.3 pH).[http://books.google.nl/books?id=HrNwdRj1ehoC&amp;amp;dq=Synedra+ulna+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s](page 877)&lt;br /&gt;
Synedra ulna is perennial, suspended in the water, current indifferent, able to tolerate small amounts of salt and develops optimally at ph &amp;gt;7. It is eutrophic, characteristic of the zone where oxidation of biodegradable compounds is complete, or oxidation is still occurring. It develops over the range of 0 to 25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] Synedra ulna may dominate in saline, alkaline waters.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Synedra+ulna+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] Alkalinity range is 37 to 170 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] Synedra ulna may thrive in water with 7.0-8.6 pH, 1.0-7.0 DO, 125-550 total alkalinity, 70-247 mg/L Cl, 10-60 mg/L sulphate, 2-5.6 mg/L phosphate, 6.9-23.5 mg/L Mg, 7.5-27.5 mg/L Ca, 1.6-9.0 mg/L nitrate, 2.5-10.2 mg/L ammonium and 17-34.5ºC[http://www.jeb.co.in/journal_issues/200601_jan06/paper_06.pdf] Synedra ulna has [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae high silicon] and low phosphorus requirements.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] Together with Rhopalodia gibba, it may grow in 1.2 ppt salinity, 10 mg/L potassium, 56.4 mg/L calcium, 21 mg/L SiO2, 5.7 meq/L alkalinity, 1.9 mg/L phosphates and 8.6 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
==Anabaena==&lt;br /&gt;
[[Image:Anabaena_cylindrica.jpg|thumb|left| Anabaena cylindrica [http://cyanobacteria.myspecies.info/sites/cyanobacteria.myspecies.info/files/Anabaena_cf._cylindrica_AUTH_0699_63x%20%2820%29.jpg © snkonsta]]]&lt;br /&gt;
Anabaena sp. are large filamentous and neurotoxin-producing cyanobacteria (blue-green algae) that exist as plankton.&lt;br /&gt;
Feeding mostly on Anabaena spiroides, Oreochromis niloticus (mean total weight: 31.5 g) may ingest 5.1% body mass equivalent (wet weight basis).[http://repository.seafdec.org.ph/handle/10862/1848]&lt;br /&gt;
Ingestion rates are higher on Anabaena cylindrica than on Microcystis aeruginosa.[http://link.springer.com/article/10.1007/BF00002160#page-1] Anabaena flos-aquae is much better assimilated (83%) than green algae. Gross growth efficiency of fish fed Anabaena flos-aquae was 46% in comparison to fish fed Chlamydomonas (22%) and Ankistrodesmus falcatus; 24%.[http://link.springer.com/article/10.1007/BF00379861#page-1] Tilapia grow much better on Anabaena than on Clamydomonas (green algae) [http://link.springer.com/article/10.1007/BF00000362#page-1] Nile Tilapia fed ration equal 3% of the total fish body weight, containing 1.5% Anabaena had higher growth and were relatively protected against A. hyrophila infection.[http://www.scopemed.org/?jft=31&amp;amp;ft=31-1378827209] Anabaena circinalis may cause off-flavor in fish flesh.[http://www.cabdirect.org/abstracts/20133297816.html] In competition experiments between Anabaeana and toxic Microcystis aeruginosa, dominancy is mainly determined by the initial biomass ratio.[http://www.researchgate.net/publication/236246459_Competition_between_toxic_Microcystis_aeruginosa_and_nontoxic_Microcystis_wesenbergii_with_Anabaena_PCC7120] Anabaena circinalis and Anabaena spiroides thrive in salinity less than 3 ppt.[http://researchonline.nd.edu.au/era_sci__article/3/] Anabaena tortulosa is relatively tolerant of salinity up to 15 ppt.[http://www.cdsewer.org/BdAct/Presentations/USU_Bioassay_Report_DRAFT_4B.pdf] Anabaena spp. (and Anabaenopsis) are common in alkaline saline lakes. [http://www.researchgate.net/publication/6971813_Toxic_cyanobacteria_and_their_toxins_in_standing_waters_of_Kenya_implications_for_water_resource_use] Salt tolerance in cyanobacteria is increased by nitrogenous compounds (inhibiting Na&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; influx), but by nitrate more so than by ammonium or glutamine.[http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&amp;amp;action=reflink&amp;amp;origin=wiley&amp;amp;version=1%2E0&amp;amp;coi=1%3ACAS%3A528%3ADyaL28Xjt1an&amp;amp;md5=25a98907beddc7831f788b4c5b39f7bf]&lt;br /&gt;
&lt;br /&gt;
==Spirulina==&lt;br /&gt;
[[Image:Spirulina.jpg|thumb|left| Spirulina platensis [http://naturalactives.com/micro-algae-fights-skin-ageing/]]]&lt;br /&gt;
[[Image:Spirulina_3.jpg|thumb|right| Arthrospira platensis [http://www.sbs.utexas.edu/utex/algaeDetail.aspx?algaeID=4383 © UT-Austin]]]&lt;br /&gt;
[[Image:Spirulina_2.jpg|thumb|left| Spirulina platensis [http://imworld.ru/priroda/spirulina-samoe-poleznoe-rastenie-na-zemle.html]]]&lt;br /&gt;
[[Image:Spirulina_4.jpg|thumb|right| Arthrospira platensis [http://fmp.conncoll.edu/Silicasecchidisk/LucidKeys/Carolina_Key/html/Arthrospira_Main.html]]]&lt;br /&gt;
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Spirulina (Oscillatoria, Arthrospira) are blue-green algae/cyanobacteria and a rich source of protein and potassium.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.2003.tb09615.x/abstract] Spirulina platensis contains 50 to 65% protein (10% is non-protein nitrogen)[http://pubs.acs.org/doi/abs/10.1021/jf00105a022], is high in vitamin B1 and B6 and is 85 to 95% assimilated. It may contain up to 14% lipids (of dry mass). [http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8#page-1] Spirulina platensis growth is optimum below 10.5 pH.[http://www.sciencedirect.com/science/article/pii/S0044848603001236] Spirulina platensis is naturally found in tropical regions inhabiting alkaline lakes (pH 9.4 to 11 ; HCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; &amp;gt; 51 meq/L [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283708/?page=5]) with high concentration of NaCl and bicarbonates [http://www.scielo.br/scielo.php?pid=S1517-83822008000100022&amp;amp;script=sci_arttext&amp;amp;tlng=pt], but may adapt to very different habitats, including freshwater.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283708/?page=2] In highly alkaline conditions (&amp;gt;50 meq/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity; 9.8-11 pH), at higher salt levels (2.5 to 30 g. salt / L; mostly carbonates and bicarbonates), Spirulina platensis becomes predominant (with Anabaenopsis, Oscillatoria, Synechocystis), or even the only significant organism (&amp;gt; 30 g. salt / L ; 10.5 &amp;lt; pH &amp;gt; 11) Optimum salinity may be between 20 and 70 ppt. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283708/?page=3] (seawater salinity is 35 ppt) Oreochromis niloticus may fairly tolerate salinity up to 10 ppt [http://www.sciencedirect.com/science/article/pii/0044848685901024] Tilapia may tolerate 0.5% salinity (5 ppt).[http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf] &lt;br /&gt;
At 8 g / L salinity adult Oreochromis niloticus may be stocked at 40 fish / m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;. At 15 g/L salinity total production is more than halved.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2006.01605.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
Oreochromis niloticus may also (barely) survive in lakes with ~27‰ salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] Oreochromis niloticus need 7.4-8.2 pH; 50-140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity and 140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;/L hardness.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
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Spirulina platensis (small biomass) may be grown on [http://www.waiwiki.org/index.php?title=Composting_toilet#Urine human urine].[http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L [http://link.springer.com/article/10.1007%2FBF02179776#page-1], the best growth temperature being 29°C [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] at an appropriate slowly increasing urea feeding rate.[http://www.sciencedirect.com/science/article/pii/S0044848604005952] &lt;br /&gt;
Regarding phosphorus, maximum biomass production at high light intensity was observed at 250 mg/L K&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;HPO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;.[http://link.springer.com/article/10.1007/s11274-012-1076-4#page-1] The optimum nitrogen:phosphorus ratio is 7:1 at optimum light intensity.[http://link.springer.com/article/10.1007/BF00016689#page-1] (in human urine N/P = 8.7:1[http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf]) Spirulina is a thermophilic microorganism with an optimal growth temperature of 35 to 37°C.[http://atarazanas.sci.uma.es/docs/tesisuma/1664380x.pdf] The temperature range for optimum Spirulina growth is 30 to 35°C.[http://link.springer.com/article/10.1023/A:1009233230706#page-1] At 35°C there is a negative effect on biomass production but a positive effect on the production of protein, lipids and phenolics.[http://www.sciencedirect.com/science/article/pii/S0960852405004761] Spirulina may be cultivated in water with 16 g/L baking soda, 2 g/L Potassium nitrate, 1 g/L sea salt, 0.1 g/L potassium phosphate and 0.01 g/L iron sulphate. [http://en.wikipedia.org/wiki/Spirulina_(dietary_supplement)] In an open raceway system dissolved oxygen may reach 30 mg/L at 28°C. Above 25 mg/L Spirulina growth is inhibited.[http://www.sciencedirect.com/science/article/pii/S0044848603001236] During a period of nitrogen starvation, Spirulina platensis may survive and grow unaffected based on/due to prior storage of nitrogen in pigment proteins (C-Phycocyanin).[http://link.springer.com/article/10.1007/BF00403211#page-1]&lt;br /&gt;
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Tilapia fed solely on raw Spirulina keep normal reproduction throughout three generations.[http://www.sciencedirect.com/science/article/pii/S0044848603008226]&lt;br /&gt;
Larval tilapia that were fed solely raw Spirulina at a feeding rate of 30% (on a dry basis) of bodyweight in the first 3 weeks, 10% in weeks 4–6, and 3% in weeks 7–10, kept growing without any abnormality[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00388.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false], at a slightly lower rate than fish fed fishmeal. Fish fed Spirulina had higher protein, polar lipid, linoleic acid and γ-linolenic acid contents and lower ash and omega-3 contents than fish fed the commercial diet.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00386.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Elastic modulus of flesh of the Spirulina-fed fish was significantly higher and viscosity lower.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2003.00653.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Larval Tilapia (Oreochromis niloticus &amp;lt; 3.4 cm) prefer consuming Spirulina platensis over Euglena gracilis (flagellate protists), and is also more readily assimilated. Tilapia prefer both species over Chlorella vulgaris (green algae, 45% dry matter protein), which is hardly ingested by larval tilapia.[http://www.sciencedirect.com/science/article/pii/S0044848604002595] Tilapia prefer Spirulina over Navicula (a diatom).[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA465&amp;amp;dq=algae+tilapia&amp;amp;ots=BVjAbOF-zV&amp;amp;sig=aEIMIY5ekRp5adhtpwsOIqPQTMQ#v=onepage&amp;amp;q=algae%20tilapia&amp;amp;f=false] In Tilapia aurea fed Spirulina platensis, food conversion was 2.0.[http://www.sciencedirect.com/science/article/pii/004484867690140X] Dietary Spirulina incorporation increases antioxidant activity in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25231739] When live Spirulina partly replaces commercial feed, fish mortality due to Aeromonas hydrophila infection decreases with an increase in the Spirulina level in the diet.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2009.02195.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
==Microcystis==&lt;br /&gt;
In lakes where Microcystis spp. are the most abundant, Microcystis sp. may account for more than 80% of ingested phytoplankton.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Various cyanobacteria may produce toxins (eg Microcystis aeruginosa). Average digestion rate of Microcystis aeruginosa (cyanobacteria producing neurotoxins and hepatotoxins) by Tilapia fingerlings is 58 to 78% at 25℃.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-SCKX200506012.htm] In Oreochromis niloticus a high rate of defaecation of undigested Microcystis may occur.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1998.tb00104.x/abstract]&lt;br /&gt;
&lt;br /&gt;
Microcystis aeruginosa may survive in a wide range of salinities.[http://link.springer.com/chapter/10.1007/978-94-009-3095-7_3#page-1] Oreochromis mossambicus exposed to extracts from Microcystis aeruginosa showed significant plasma hypocalcaemia.[http://jeb.biologists.org/content/199/6/1319.short] Chronic exposure to toxin producing cyanobacteria (Microcystis aeruginosa) may not (115 days trial[http://www.sciencedirect.com/science/article/pii/S0041010109001925], 90 days trial[http://www.sciencedirect.com/science/article/pii/S004484860600648X]) negatively affect growth, feed conversion efficiency, health or mortality in Oreochromis niloticus. Microcystins do accumulate in muscle tissue (and particularly in the liver), which may exceed the upper limit of the tolerable daily intake of microcystins suggested by the WHO (0.04 μg/kg body weight/d).[http://www.sciencedirect.com/science/article/pii/S004484860600648X] Accumulation of microcystis increases lipoxidation (a biomarker of oxygen-mediated toxicity) in the liver.[http://www.sciencedirect.com/science/article/pii/S0166445X0600021X] Microcystin concentrations in the liver and intestine may gradually decrease as a result of the capacity in Tilapia to depurate and excrete microcystins into the bile and surrounding water, to avoid toxicity.[http://www.sciencedirect.com/science/article/pii/S0147651305000266] During the depuration period, muscle tissue may contain the highest concentration of microcystins, which may exceed the tolerable limit for humans (even in combined diets).[http://www.sciencedirect.com/science/article/pii/S0166445X04002279] Microcystis wesenbergii is non-toxic, less competitive, but more suited to low phosphorus environments than Microcystis aeruginosa.[http://www.researchgate.net/publication/236246459_Competition_between_toxic_Microcystis_aeruginosa_and_nontoxic_Microcystis_wesenbergii_with_Anabaena_PCC7120] Microcystis flos-aquae is more dominant in salinity above 3 ppt, whereas Microcystis aeruginosa is dominant in salinity up to 3 ppt.[http://researchonline.nd.edu.au/era_sci__article/3/]&lt;br /&gt;
&lt;br /&gt;
==Periphyton==&lt;br /&gt;
Periphyton is a mixture of algae, cyanobacteria, heterotrophic microbes and detritus (mainly scraps of macrophytes and mud [http://www.cabdirect.org/abstracts/20023142580.html]) attached to submerged surfaces embedded in a slimy mucopolysaccharide matrix.[http://link.springer.com/article/10.1023/A:1022639805031#page-1] Oreochromis niloticus graze actively, with little selectivity, on the periphyton established on artificial substrates in cages. Both animal and plant material are removed to an equal extent, but less smaller particles are ingested.[http://www.sciencedirect.com/science/article/pii/S0044848699003658] Surface-grazing on periphyton is greater than on Microcystis aeruginosa. For tilapia, filter-feeding may be a relatively unimportant method of ingesting algae.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1993.tb00573.x/abstract] Tilapia mossambica feeds almost exclusively on periphytic detrital aggregate. (Assimilation efficiencies: organic matter 63%, protein 77%, carbohydrate 63%) [http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1981)110%3C239%3ADAAOPD%3E2.0.CO%3B2#.VCusqPl_s10] Feed conversion ratio of dry matter periphyton may be 2.81. Fish growth may be sustained on periphyton despite 55% (dry matter) ash content.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2003.00802.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] The fresh microbial mat was 81% digestible by Nile tilapia, comparing favorably with commercial catfish feed in digestibility by Nile tilapia. The dried form was significantly less digestible.[http://www.tandfonline.com/doi/abs/10.1577/1548-8640(1989)051%3C0083%3ADOACFF%3E2.3.CO%3B2#.VCu6hvl_s10] Tilapia are equipped with a &amp;#039;stomach bypass&amp;#039; to be able to bypass or regurgitate unwanted materials.[http://www.researchgate.net/publication/229614655_A_study_of_the_histology_of_the_digestive_tract_of_the_Nile_tilapia]&lt;br /&gt;
&lt;br /&gt;
==Algae meal==&lt;br /&gt;
Under most unnatural feeding conditions tilapia are unable to sufficiently ingest high volumes of algae. They may need constant grazing to fulfill their nutrient requirements.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1995.tb01868.x/abstract] Tilapia (Sarotherodon niloticus) grow better on fishmeal (fish meal may contain 74% protein and 8% lipids.[http://link.springer.com/article/10.1007/s10499-008-9207-5#page-1]) than on a 25% protein green algae meal (Cladophora glomerata). Weight gain decreased as the level of algal protein increased as replacement of fish meal. Protein digestibility was highest on a 5:1 ratio (fishmeal : green algae meal).[http://www.sciencedirect.com/science/article/pii/0044848683901485] Protein synthesis (with normal sulfur and carbon content) by green algae during the night may match protein synthesis during the day (in Dunaliella tertiolecta).[http://m.aslo.info/lo/toc/vol_29/issue_4/0731.pdf] Protein derived from algae does not promote adequate growth in Rainbow trout.[http://www.sciencedirect.com/science/article/pii/0044848678900972] Fish fed 5% ulva meal (Green algae; Ulva rigida) showed increased growth, feed conversion ratio and protein efficiency ratio.[http://link.springer.com/article/10.1007/s10499-008-9207-5#page-1] Ulva meal may replace soy bean meal to the extent of 20% without negatively affecting growth of male larval tilapia. Feed conversion ratio increased with increasing ulva meal content.[http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0426.2007.01017.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Green algae meal (Hydrodictyon reticulatum) may replace meal to the extent of 25% without negatively affecting growth of Oreochromis niloticus and Tilapia zillii fingerlings.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1985.tb04034.x/abstract] Spirulina maxima meal protein can replace up to 40% of the fish meal protein in Oreochromis mossambicus fry diets without negatively affecting growth.[http://www.researchgate.net/publication/230241662_Effect_of_the_use_of_the_microalga_Spirulina_maxima_as_fish_meal_replacement_in_diets_for_tilapia_Oreochromis_mossambicus_(Peters)_fry]&lt;br /&gt;
&lt;br /&gt;
==Off-flavor==&lt;br /&gt;
Cyanobacteria causing off-flavor (by geosmin and 2-methylisoborneol, MIB in fish flesh) are Oscillatoria tenuis, Anabaena circinalis and Pseudanabaena catenata. Tilapia flesh derived from cage culture provided a lower possibility of off-flavor contamination than those from earthen ponds.[http://www.cabdirect.org/abstracts/20133297816.html]&lt;br /&gt;
Geosmin (1,10-trans-dimethyl-trans-9-decaol) in fresh tilapia at concentrations ranging from 7.55 to 9.85 μg/kg of fish flesh is detected as &amp;#039;muddy flavor&amp;#039; by sensory evaluation. In brackish fish, only geosmin levels below 2.6 μg/kg remain undetected. The highest geosmin concentrations are contained in the intestines, followed by abdominal, skin and muscle tissues, subsequently. It takes at least 16 days in static clean water to eliminate the muddy flavor from geosmin absorbed through the skin.[http://www.tandfonline.com/doi/abs/10.1300/J030v09n02_04#.VHywhYuG810]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Algae_for_Tilapia&amp;diff=4449</id>
		<title>Algae for Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Algae_for_Tilapia&amp;diff=4449"/>
		<updated>2014-12-29T14:14:34Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;* Plankton are organisms (animal or plant) that live in the water and cannot swim against a current; zooplankton and phytoplankton.&lt;br /&gt;
* Algae includes brown and green algae (planktonic or attached; single-celled or multicellular), and diatoms (planktonic, attached or motile; single-celled or colonial). &lt;br /&gt;
* Blue-green algae (planktonic or attached; single-celled or colonial) are bacteria with photosynthetic capacity; cyanobacteria (cyano = blue).&lt;br /&gt;
&lt;br /&gt;
==Feeding==&lt;br /&gt;
[[Image:Ceriodaphnia_reticulata.jpg|thumb|right| Ceriodaphnia [http://www.micromagus.net/microscopes/pondlife_cladocera.html © micromagus.net]]]&lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Tilapia Tilapia] start feeding shortly before dawn and feed continually (but most intensively between 12:00 and 18:00[http://cabdirect.org/abstracts/20013100658.html;jsessionid=1F937711ACC14AC20505C4692DBC7CB3]) until about dusk. They do not feed during the night.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-7998.1973.tb07513.x/abstract][http://link.springer.com/chapter/10.1007/978-94-009-7281-0_13#page-1] Tilapia (Oreochromis niloticus) may effectively control algal blooms in eutrophic waters.[http://link.springer.com/article/10.1007/s10750-006-0023-5#page-1] Planktonic plants and &lt;br /&gt;
animals make up the bulk of the diet under natural conditions.[http://www.nativefishlab.net/library/textpdf/17926.pdf] Zooplankton (animal) does not exceed 1.5% of total stomach contents [http://www.cabdirect.org/abstracts/20023142580.html], with predation pressure being the greatest on Ceriodaphnia reticulata (small water fleas that feed on small algae)   [http://link.springer.com/article/10.1007/BF00016658#page-1], due to low escape probabilities for (Cerio)daphnia compared to copepods (small crustaceans).[http://www.nrcresearchpress.com/doi/abs/10.1139/f78-215#.VF5gm_mG_QM]&lt;br /&gt;
Phytoplankton (plant plankton) may contribute over 30% by volume of stomach contents of Oreochromis niloticus.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Small Tilapia rendalli consumed more diatoms than larger individuals.[http://aquaticcommons.org/9103/] Oreochromis niloticus feeds mainly on zooplankton until 5 cm long, and mainly on phytoplankton as it grows older. In small fish (&amp;lt;75 g.) Bacillariophyceae are the main phytoplankter, and in larger fish Chlorophyceae are the main phytoplankter.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/500.pdf] Tilapia (Sarotherodon melanotheron) shift from visually feeding on zooplankton when juveniles to mostly filter feeding on phytoplankton when adults [http://www.sciencedirect.com/science/article/pii/S0144860908000666], consuming less Microcystis and Scenedesmus as they grow bigger.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2003.00917.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Blue-green algae (colonies of photosynthetic bacteria; cyanobacteria) are common components of the Tilapia diet. In the stomach of Tilapia nilotica the cells of blue-green algae are lysed by high concentrations of acid (pH 1.4–1.9). After lysis, cell contents are digested in the intestine (by pepsinogen, a pancreatic α-amylase, trypsin, chymotrypsin and esterase activity). Acid is secreted in relation to feeding. Acid is not secreted by stressed fish.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-7998.1973.tb07514.x/abstract] Filtration rates of algae increases linearly as water temperature increases from 17°C to 32°C.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2003.00830.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
The main species of algae found in Oreochromis niloticus stomach belonged to Cyanophyta (2nd most ammonium tolerant[http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae]), Chlorophyta (most ammonium tolerant[http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae]), Bacillariophyta and Euglenophyta.[http://www.cabdirect.org/abstracts/20023142580.html] The most frequent species represented in Oreochromis niloticus stomach are Anabaena sp., Merismopedia eleganse, Microcystis aeruginose, Nodularia harveyana and Oscillatoria sp. (Cyanophyta), Cerasterias sp., Chlorella spp., Crucigenia sp., Pediastrum spp., Scenedesmus spp. and Tetraedron sp. (Chlorophyta), Amphora ovalis, Cocconeis placentula, Cymatopleura solea, Cymbella cistula, Gyrosigma attenuatum, Melosira granulata, Navicula spp., Nitzschia spp., Pinnularia spp., Serurella sp. and Synedra sp. (Bacillariophyta) and Euglena and Phacus spp. (Euglenophyta).[http://www.cabdirect.org/abstracts/20023142580.html] The most frequent genera represented in fish (Oreochromis niloticus) stomach in all sizes were Anabaena, Anabaenopsis, Coleospharium, Merismopedia, Microcystis, Nodularia, Oscillatoria, Spirulina (Cyanobacteria), Actinostrum, Chlorella, Closterium, Coelastrum, Eudrina, Pandorina, Pediastrum, Scenedesmus, Shereoderia, Staurastrum (Chlorophyceae), Amphora, Cocconeis, Cymatopleura, Cymbella, Gyrosigma, Melosira, Navicula, Pinnularia, Synedra (Bacillariophyceae) and Euglena and Phacus (Euglenophyceae).[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/500.pdf] Botryococcus sp. (green algae)[http://link.springer.com/article/10.1007/BF00008157#page-1][http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1987.tb05767.x/abstract] and Melosira (a diatom; Bacillariophyceae)[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1993.tb00396.x/abstract] may also be major food items in the diet of Oreochromis niloticus.&lt;br /&gt;
&lt;br /&gt;
==Selective feeding==&lt;br /&gt;
[[Image:Uroglenopsis.jpg|thumb|right| Uroglenopsis [http://cfb.unh.edu/phycokey/Choices/Chrysophyceae/colonial_chrysophyceae/flagellated/UROGLENOPSIS/Uroglenopsis_Image_page.html © John DuFresene]]]&lt;br /&gt;
[[Image:Spirulina_platensis.jpg|thumb|left| Spirulina platensis [http://mcc.nies.go.jp/images/100images/nies-0046.jpg © http://mcc.nies.go.jp/]]]&lt;br /&gt;
[[Image:Ceratium.jpg|thumb|right| Ceratium [http://protist.i.hosei.ac.jp/pdb/images/Mastigophora/Ceratium/furca_7.html © Y. Tsukii]]]&lt;br /&gt;
&lt;br /&gt;
Tilapia feed selectively on large algae, mainly cyanobacteria and diatoms [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2427.2005.01407.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] and select more for diatoms than for the blue-green algae (cyanobacteria).[http://oceandocs.net/bitstream/1834/1160/1/9290640789-P93103.pdf]&lt;br /&gt;
Oreochromis niloticus may select only Cyanophyta and Euglenophyta (unicellular flagellate protozoa), with occasional Bacillariophyta (diatoms).[http://www.cabdirect.org/abstracts/20013148595.html;jsessionid=0F8AC1DEC89977E92FA074A1E5D3E898] &lt;br /&gt;
Oreochromis niloticus may select Cyanophyta and sometimes select Bacillariophyta and Euglenophyta.[http://www.cabdirect.org/abstracts/20023142580.html] &lt;br /&gt;
Blue tilapias (4.3 to 18.7 cm long) selectively feed on particles larger than 25 μm.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1984)113%3C397%3APAPCIO%3E2.0.CO%3B2#.VCvJePl_s10] &lt;br /&gt;
Nile tilapia is particularly effective in filtering the larger particle size taxa.[http://www.sciencedirect.com/science/article/pii/S0044848602001333] &lt;br /&gt;
Larger phytoplankton (plant plankton) are being filtered proportionally more than the smaller phytoplankton, and cyanobacteria (in 100% of fish[http://www.sciencedirect.com/science/article/pii/0044848678900157]) more than green algae (Scenedesmus, Ankistrodesmus, Tetraedron).[http://www.sciencedirect.com/science/article/pii/S0044848602006142] &lt;br /&gt;
Tilapia aurea (Blue tilapia) favour Uroglenopsis sp. (cells forming hollow spherical colonies, &amp;gt; 400 μm diameter), Ceratium sp.(dinoflagellate (the least ammonium tolerant [http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae]), 310 μm long, 58 μm wide; optimum salinity 10 ppt[http://link.springer.com/article/10.1007/BF00346767#page-1]) and Keratella sp. over (small-sized algae) Rhodomonas sp., Chrysochromulina sp., Chlamydomonas sp., Cyclotella sp. and (zooplankter) Diaptomus sp.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1984)113%3C397%3APAPCIO%3E2.0.CO%3B2#.VCvJePl_s10] Oreochromis niloticus juveniles prefer Elodea canadensis over Potamogeton pectinatus and Spirodela polyrhiza. Myriophyllum spicatum was the least preferred.[http://agris.fao.org/agris-search/search.do?recordID=CZ2005000021]&lt;br /&gt;
&lt;br /&gt;
Diatoms are microscopic unicellular algae, all consisting of the same basic parts; nucleus, cytoplasm, plasma membrane, and the cell wall.[http://www.modernmicroscopy.com/main.asp?article=89&amp;amp;print=true&amp;amp;pix=true] Oreochromis niloticus prefer to prey on the large size diatoms, such as Melosira granulata, Synedra ulna, Cyclotella ocellata and Cyclotella operculata.[http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] &lt;br /&gt;
Astrionella sp. and Rhopalodia vermicularis (bacillariophytes) and Peridinium sp. (dinophyte) were selected by both wild and pond-cultured Oreochromis niloticus. Lake fish particularly selected Astrionella sp., Aulacoseira (Melosira) nyassensis, Navicula sp. (which grow on sediment[http://www.scribd.com/doc/41415598/The-Epipelic-Diatom-Community-at-the-Downstream-Rivers-of-Torong-Galeh-and-Legi-Semarang-Indonesia]) and Rhopalodia vermicularis (bacillariophytes). Pond fish particularly selected Ankistrodesmus falcatus (chlorophyte), Lyngbya circumcreta (cyanophyte) and Nitzschia acicularis.[http://www.tandfonline.com/doi/abs/10.2989/16085914.2013.784698#.VFu___mG_QM] Oreochromis niloticus may prefer Spirulina laxisma and Nitzschia accicularis the most. The least preferred food items may be Lyngbya circumcreta, Microcystis aeruginosa and Pediastrum simplex.[http://www.oceandocs.org/handle/1834/1160]&lt;br /&gt;
&lt;br /&gt;
==Algal growth==&lt;br /&gt;
The favourable pH range for plankton growth ranges between 6.5 and 7.5. Acidic conditions favor chlorophytes (green algae) while alkaline condition fosters nitrogen-fixing cyanobacteria.[http://books.google.nl/books?id=u-NNxcC8Fn0C&amp;amp;dq=%22Royal+Academy+of+Overseas+Sciences%22&amp;amp;ie=ISO-8859-1&amp;amp;source=gbs_gdata&amp;amp;redir_esc=y] High salinity favors green algae (plus Spirulina) while low salinity fosters cyanobacteria. In phytoplankton-based recirculating systems, algae may rather be limited by dissolved organic carbon availability rather than by nitrogen or phosphorus.[http://www.sciencedirect.com/science/article/pii/S0144860908000666] Chlorella vulgaris has higher lipid contents than Scenedesmus abundans and Monoraphidium minitum, which was also reflected in their predator, Brachionus calyciflorus. [http://www.sciencedirect.com/science/article/pii/S0044848699000137] Organic matter of manure (eg chicken manure [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract]) hardly contributes directly to fish growth; only indirectly through carbon incorporated in algae.[http://www.sciencedirect.com/science/article/pii/0044848690901154] Most fertilized fish ponds with dirty green water have more than 242 mg phytoplankton biomass per L water.[http://ppmq.ars.usda.gov/research/publications/publications.htm?seq_no_115=148778] Diatoms are less dependent on trace elements than other algae.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
&lt;br /&gt;
==Brachionus plicatilis==&lt;br /&gt;
[[Image:Brachionus_plicatilis.jpg|thumb|left| Brachionus plicatilis ]]&lt;br /&gt;
[[Image:Akashiwo_sanguinea.jpg|thumb|right| Akashiwo sanguinea [http://www.serc.si.edu/labs/phytoplankton/guide/addtl_collections/Cape%20Cod/GymsanCC.aspx © SERC]]]&lt;br /&gt;
&lt;br /&gt;
Brachionus plicatilis is an important food source for larval fish. Brachionus plicatilis is also the most successful species of zooplankton used to control unicellular algal contaminants in Spirulina mass cultures even under conditions adverse to the growth of Spirulina (very low bicarbonate).[http://onlinelibrary.wiley.com/doi/10.1002/bit.260300205/abstract]&lt;br /&gt;
Brachionus plicatilis is a rotifer / zooplankton associated with saline, alkaline waters.[http://www.avto.aslo.info/lo/toc/vol_18/issue_1/0053.pdf] Brachionus plicatilis may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] It may be 123 to 292 μm long and 114 to 199 μm wide. Compared to temperature and salinity, strain is the most important factor determining size.[http://www.sciencedirect.com/science/article/pii/0044848684903004] Maximum reproduction occurs between 30 and 34 C. Reproduction rate is similar in 25% and 33% seawater. Brachionus plicatilis may thrive in 0.5 to 30 ppt salinity (brackish) and may be 99 to 281 μm long and may weigh 0.18 μg. In contrast to female Brachionus rubens, fecund female Brachionus plicatilis do not attach to a substrate. [http://link.springer.com/article/10.1007/BF00354834#page-1] Adult Brachionus plicatilis may be 213 to 315 μm long. Optimum salinity may be 18 ppt. The most common feed types are single-celled algae.[http://nsgl.gso.uri.edu/hawau/hawauw91001/hawauw91001_part5.pdf] Brachionus plicatilis may (together with Nitzschia spp) survive in 10 pH, high calcium carbonate and 91 ppt salinity.[http://link.springer.com/article/10.1007/BF00025190#page-1]&lt;br /&gt;
&lt;br /&gt;
Brachionus plicatilis may be mass cultured on Akashiwo sanguinea (Gymnodinium splendens / nelsoni), an unarmored (naked) dinoflagellate. [http://link.springer.com/article/10.1007/BF00354834#page-1] Akashiwo sanguinea is a mixotroph, as it is both photosynthetic and feeds on cyanobacteria and nanociliate populations (tiny  protozoans)[http://link.springer.com/article/10.1007%2FBF00350065], which may be algivores.[http://link.springer.com/article/10.1007/BF00877447#page-1]&lt;br /&gt;
It may form subsurface chlorophyll maximum layers in the water, which is consumed by larval fish.[http://www.aslo.org/lo/toc/vol_23/issue_2/0219.html] This may be in response to a relative nitrate deficiency during the light period.[http://link.springer.com/article/10.1007%2FBF00388169] Dinoflagellates prefer calm water column situations; small-scale turbulence may diminish dinoflagellate growth and cell division, but may also increase cell size.[http://digital.csic.es/handle/10261/40987] Akashiwo sanguinea may thrive in brackish conditions.[http://link.springer.com/article/10.1007/s12040-011-0073-6#page-1] Coastal Akashiwo sanguinea bloom events may be supported by elevated urea.[http://www.sciencedirect.com/science/article/pii/S156898830800108X]&lt;br /&gt;
&lt;br /&gt;
==Keratella==&lt;br /&gt;
[[Image:Keratella.jpg|thumb|left| Keratella [http://www.plingfactory.de/Science/Atlas/KennkartenTiere/Rotifers/01RotEng/source/Keratella%20quadrata.html © Michael Plewka]]]&lt;br /&gt;
&lt;br /&gt;
Young Oreochromis niloticus mainly feed on zooplankton until ~5 cm long.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/500.pdf] &lt;br /&gt;
Keratella sp. are relatively small (~100 μm long) &amp;#039;wheel animals&amp;#039; / rotifers / zooplankton. Growth may be optimal at 24°C temperature and 20 ppt salinity. Fecundity may be optimal at 0 ppt salinity.[http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=SSHGB2_2013_v25n5_1205] &lt;br /&gt;
&lt;br /&gt;
Keratella cochlearis may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Keratella cochlearis and Keratella quadrata are euryhaline and perennial (not seasonal).[https://bora.uib.no/handle/1956/2511]&lt;br /&gt;
Keratella cochlearis, Keratella americana and Keratella tropica are considered euryhaline (able to adapt to a wide range of salinities). Keratella hispida may thrive on 30 ppt.[http://link.springer.com/article/10.1007%2FBF00006515#page-1] &lt;br /&gt;
Keratella tropicana may occur in shallow waters at 43 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf]&lt;br /&gt;
&lt;br /&gt;
==Peridinium cinctum==&lt;br /&gt;
[[Image:Peridinium_cinctum.jpg|thumb|right| Peridinium cinctum [http://www.photomacrography.net/forum/viewtopic.php?p=99321&amp;amp;sid=7c740085cef2cf3d31b31c57693122ad © Jacek]]]&lt;br /&gt;
&lt;br /&gt;
Peridinium species are large phytoplankton (30-70 μm diameter armoured dinoflagellates) that less efficiently take up nutrients than smaller species. At their advantage is the capacity to modify their position in the water column (in accordance with light and nutrient availability), that they are (because of their large size) inedible by most zooplankton, and their extremely low phosphorus requirements (C:P molar ratio is 460:1).[http://link.springer.com/article/10.1007/s10750-012-1145-6#page-1] They may produce 2 to 3 times more organic matter than other alga.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=GkoJTvXNuggC&amp;amp;oi=fnd&amp;amp;pg=PA167&amp;amp;dq=Peridinium+cinctum+salinity&amp;amp;ots=1XAXxWCTsT&amp;amp;sig=nbgtovZiLf9cFuxG03EkuQKeokc#v=onepage&amp;amp;q=Peridinium%20cinctum%20salinity&amp;amp;f=false]&lt;br /&gt;
&lt;br /&gt;
Tilapia (galilaea) favourably consume Peridinium cinctum.[http://www.sciencedirect.com/science/article/pii/0044848676900478] Feeding rates of Tilapia galilaea and Tilapia aurea reach maximum values for zooplankton (animal plankton) and Peridinium cinctum.[http://www.nrcresearchpress.com/doi/abs/10.1139/f88-082#.VCwiZfl_s10] Fingerlings fed experimentally on chlorophytes had lower growth rates compared with mosquito larvae, zooplankton and fresh Peridinium.[http://www.sciencedirect.com/science/article/pii/0044848680900393] Growth rates of age-1 Tilapia galilaea on Peridinium may be 1–2% mean weight per day.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2427.1979.tb01483.x/abstract] &lt;br /&gt;
&lt;br /&gt;
When phosphorus is low, Peridinium has an advantage over other alga.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=GkoJTvXNuggC&amp;amp;oi=fnd&amp;amp;pg=PA167&amp;amp;dq=Peridinium+cinctum+salinity&amp;amp;ots=1XAXxWCTsT&amp;amp;sig=nbgtovZiLf9cFuxG03EkuQKeokc#v=onepage&amp;amp;q=Peridinium%20cinctum%20salinity&amp;amp;f=false] Peridinium aciculiferum may particularly produce toxins when deprived of phosphorus and in stationary phase.[https://lup.lub.lu.se/search/publication/146608] Blooms of Peridinium gatunense (biomass &amp;gt; 100 g / m2) may change the color of the water to coffee brown in distinct patches and are associated with excessive oxygen super-saturation, and high phosphorus (&amp;gt; 0.05 mg/L) and nitrogen (&amp;gt; 1 mg/L).[http://link.springer.com/chapter/10.1007/978-94-017-8944-8_11#page-1] Peridinium gatunense and Microcystis sp. are highly competitive.[http://www.ncbi.nlm.nih.gov/pubmed/12401172] Peridinium ponticum may thrive in 17 ppt salinity.[http://www.sciencedirect.com/science/article/pii/S0025322702003481] Peridinium cinctum is common below 20 ppt salinity.[http://link.springer.com/article/10.1007/BF00025173#page-1]&lt;br /&gt;
&lt;br /&gt;
==Cocconeis placentula==&lt;br /&gt;
[[Image:Cocconeis_placentula.jpg|thumb|left| Cocconeis placentula [https://www.flickr.com/photos/microagua/2634071221/ © Proyecto Agua]]]&lt;br /&gt;
[[Image:Cocconeis_placentula_2.jpg|thumb|right| Cocconeis placentula [http://nivalis.jp/kibun/microscope/gallery/cocconeis/cocconeis.html © 珪藻ギャラリートップへ]]]&lt;br /&gt;
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Oreochromis niloticus preferentially selects diatoms over cyanobacteria.[http://oceandocs.net/bitstream/1834/1160/1/9290640789-P93103.pdf]&lt;br /&gt;
&amp;#039;&amp;#039;Cocconeis placentula&amp;#039;&amp;#039; is frequently represented in Oreochromis niloticus stomach.[http://www.cabdirect.org/abstracts/20023142580.html]&lt;br /&gt;
Cocconeis placentula is a diatom that may be 9-68 µm long and 7-32 µm wide [http://westerndiatoms.colorado.edu/taxa/species/cocconeis_placentula], or up to 98 µm long and 40 µm wide.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13160100.html]&lt;br /&gt;
Cocconeis placentula thrives in moderately saline and moderately alkaline, shallow waters.[http://in-africa.org/wp-content/uploads/2012/12/Owen-et-al-1982-Nature-Holocene-L-Turkana1.pdf] &lt;br /&gt;
Cocconeis placentula readily attaches to substrates due to the secretion of adhesive substances.[http://botanika.bf.jcu.cz/thesis/pdf/Mares%20_bc06.pdf]&lt;br /&gt;
Cocconeis placentula is very common in (brackish / freshwater[http://files.figshare.com/1704702/Table_S6.pdf]) eutrophic [http://www.researchgate.net/publication/237052676_The_Epipelic_Diatom_Community_at_the_Downstream_Rivers_of_Torong_Galeh_and_Legi_Semarang_(Indonesia)], benthic habitats, where it (solitary) attaches to rocks, macrophytes and algae. It is tolerant of moderate organic pollution, with var. euglypta being possibly more tolerant.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13160100.html] Cocconeis placentula may thrive in shallow waters at 6-29°C and 6.9-7.7 pH, with 0-2.5 ppt salinity, 3.0-4.1 meq/L alkalinity, 0.0-0.45 mg/L NH&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;, 0.0-0.09 mg/L phosphates.[http://www.imp-du.com/downloadRadovi/Jasprica_Hafner%20-%20diatoms.pdf] Cocconeis placentula may prefer elevated total phosphates.[http://botanika.bf.jcu.cz/thesis/pdf/Mares%20_bc06.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cocconeis placentula var. euglypta&amp;#039;&amp;#039; is a bit smaller, but also attached solitary, and may survive in lakes with 40 ppt salinity [http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] or brackish / freshwater.[http://files.figshare.com/1704702/Table_S6.pdf]&lt;br /&gt;
&lt;br /&gt;
==Cyclotella==&lt;br /&gt;
[[Image:Ciclotella_ocellata.jpg|thumb|right| Cyclotella ocellata [http://www.redorbit.com/images/pic/28990/diatom-species-cyclotella-ocellata-is-the-most-common-planktonic-diatom-in-lake/ © RedOrbit]]]&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus prefer to prey on the large size diatoms, such as &amp;#039;&amp;#039;Cyclotella ocellata&amp;#039;&amp;#039; and Cyclotella operculata, which are Centrophycidae.[http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] &lt;br /&gt;
&amp;#039;&amp;#039;Cyclotella ocellata&amp;#039;&amp;#039; is cylinder-shaped and may have a diameter of 6 to 25 µm [http://www.planktonforum.eu/index.php?id=33&amp;amp;no_cache=1&amp;amp;L=1&amp;amp;tx_pydb_pi1%5Bzellform%5D=&amp;amp;tx_pydb_pi1%5Bdetails%5D=2021&amp;amp;tx_pydb_pi1%5Bstart%5D=60&amp;amp;tx_pydb_pi1%5Bcur%5D=12&amp;amp;cHash=b812acb971a1b23c82036790bfe9a281] up to 40 µm. (incl. &amp;#039;&amp;#039;C. krammeri&amp;#039;&amp;#039; and &amp;#039;&amp;#039;C. rossii&amp;#039;&amp;#039;) [http://www.bgbm.org/sites/default/files/documents/cediatom117Knie%2BHuebener.pdf] and may be 6.3-16.5 µm long.[http://westerndiatoms.colorado.edu/taxa/species/cyclotella_rossii] Cyclotella ocellata is sometimes considered to be planktonic (floating in the water)[ftp://ftp.ncdc.noaa.gov/pub/data/paleo/paleolimnology/lakelevels/former_ussr/lake_descriptions/textfiles/doodn.txt] or epiphytic (growing on plants). It may be considered oligosaline (0.5 to 5 ppt salinity) [http://books.google.nl/books?id=-1j-4Gx_WRoC&amp;amp;dq=Cyclotella+ocellata+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]p37), with a brackish-freshwater salinity optimum. [http://books.google.nl/books?id=SpuPKw7zZGAC&amp;amp;dq=Cyclotella+ocellata+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]p194) Cyclotella ocellata may dominate in cold waters with low salinity and very little nutrients [https://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=2&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CC0QFjAB&amp;amp;url=http%3A%2F%2Ffegi.ru%2Felibrary%2Felibrary%2Fdoc_download%2F261-morphogenesis-in-cyclotella-ocellata-complex-from-lake-elgygytgyn-chukchi-peninsula-during-the-pl&amp;amp;ei=zTprVPf2Ks7-sATFh4DoDg&amp;amp;usg=AFQjCNHOgJ8dtBLbC-_R5iDc_GRS7rDKjA&amp;amp;sig2=ovEJkM3_4hpvi9x_YjmQuA], but may also thrive in eutrophic waters [http://onlinelibrary.wiley.com/doi/10.1002/iroh.199900041/abstract], at high salinity and turbidity (in shallow waters) [http://www.schweizerbart.de/papers/algol_stud/detail/123/74435/Phytoplankton_seasonality_of_a_shallow_turbid_lake] and at higher temperatures (in diluted estuaries).[https://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=1&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CCYQFjAA&amp;amp;url=http%3A%2F%2Fwscholars.com%2Findex.php%2Fajhe%2Farticle%2Fview%2F80&amp;amp;ei=b1lrVOLmJZPSaJmQgvAI&amp;amp;usg=AFQjCNF6ywGC-0FvelqAu1dLC4vaTylVYQ&amp;amp;sig2=PBdO0SJqZSB84lqXKvuTMA&amp;amp;bvm=bv.79908130,d.d2s] Alkalinity range is 80 to 190 CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; mgl/L.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cyclotella operculata&amp;#039;&amp;#039; (5-43 µm diameter, &amp;lt;3 µm depth [http://craticula.ncl.ac.uk/EADiatomKey/html/taxon11.html]) is considered planktonic [ftp://ftp.ncdc.noaa.gov/pub/data/paleo/paleolimnology/lakelevels/former_ussr/lake_descriptions/textfiles/rudush.txt] and may thrive at 7.9 to 8.4 pH (Cl 64 mg/L ; NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; 0.28 mg/L ; P 0.23 mg/L).[http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1] Cyclotella in general thrive in the oligo-mesotrophic range (&amp;#039;deficient&amp;#039; to moderate levels of dissolved nutrients, and high DO) [http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf], but also Cyclotella operculata may dominate in eutrophic (soft and hard) waters, at 27-33°C, 40-67 mg/L alkalinity, 0.5-2.5 mg/L phosphates and 0.2-0.8 mg/L nitrates.[https://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=1&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CCgQFjAA&amp;amp;url=http%3A%2F%2Fwww.scirp.org%2Fjournal%2FPaperDownload.aspx%3FpaperID%3D4797&amp;amp;ei=k2VrVNjBJdLgapjogPAN&amp;amp;usg=AFQjCNGYT8Zpy2bMf8WRU5xXznzJY69JNw&amp;amp;sig2=2UTCg64Xwe6Cg2vWmpBPmQ&amp;amp;bvm=bv.79908130,d.d2s]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cyclotella meneghiniana&amp;#039;&amp;#039; (15 µm diameter; benthic[http://www.diatomloir.eu/Diatodouces/araphid.html]) is favoured by high light penetration [https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] and most abundant at high nitrate (4.6 mg/L). (ph 7.7 to 8.0)[http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1] Cyclotella meneghiniana may thrive in shallow water (&amp;lt;50 cm) at 908-1100 mg/L Na; 22-33 mg/L K; 7-8 mg/L Ca; 4-6 mg/L Mg; 3-4 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 0.4 mg/L Fe; 45-48 SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 674-830 mg/L Cl and 25.5-26.5 meq/L alkalinity (carbonates plus bicarbonates).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cyclotella pseudostellagera&amp;#039;&amp;#039; (8 µm diameter; euryhaline[http://www.diatomloir.eu/Diatodouces/araphid.html]) may thrive at low Cl (7 mg/L) and high P (59 mg/L, as PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;), 023 mg/L nitrate, 0.26 mg/L NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1]&lt;br /&gt;
&lt;br /&gt;
==Cymbella lanceolata==&lt;br /&gt;
[[Image:Cymbella_lanceolata.jpg|thumb|left| Cymbella lanceolata [http://www.photomacrography.net/forum/viewtopic.php?p=97973&amp;amp;sid=a34b38350a76c560037bf71cfbbe06c6 © Chris_M]]]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Cymbella lanceolata&amp;#039;&amp;#039; (aka Frustulia lanceolata, Navicula lanceolata) may be 60-220 µm long and 18-32 µm wide, is alkaliphilous (at pH &amp;gt; 7), not motile [http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13220330.html] and is widely distributed (Arctic, China to Brazil).[http://www.algaebase.org/search/species/detail/?species_id=31225] It is a shallow-water species [http://books.google.nl/books?id=ECwlAQAAIAAJ&amp;amp;dq=Cymbella+lanceolata+alkalinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]pE7) and grows solitary or attached (cell-to-cell) in bushy colonies in brackish / freshwater.[http://files.figshare.com/1704702/Table_S6.pdf] Cymbella lanceolata may thrive in shallow waters at 6-29°C and 6.9-7.7 pH, with 0-2.5 ppt salinity, 3.0-4.1 meq/L alkalinity, 0.0-0.45 mg/L ammonium, 0.0-0.09 mg/L phosphates.[http://www.imp-du.com/downloadRadovi/Jasprica_Hafner%20-%20diatoms.pdf]&lt;br /&gt;
&lt;br /&gt;
==Diatoma vulgaris==&lt;br /&gt;
[[Image:Diatoma_vulgare_colony.jpg|thumb|right| Diatoma vulgare [http://www.alamy.com/stock-photo-Diatoma-vulgare-Diatoma-vulgare-with-phase-contrast-MRI-9524969.html © Alamy]]]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Diatoma vulgaris&amp;#039;&amp;#039; is 15-60 µm long and 8-12 µm wide and may develop in fresh and brackish water. It forms zig-zag colonies attached to the substrate by a mucilage pad. [http://westerndiatoms.colorado.edu/taxa/species/Diatoma_vulgaris] Diatoma vulgaris is associated with Melosira species and may develop optimally in running water.[http://archive.org/stream/ecologyofalgaesy00tryo/ecologyofalgaesy00tryo_djvu.txt] Diatoma elongatum may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Diatoma vulgaris is considered to be mesotrophic (associated with moderate levels of suspended nutrients).[http://www.researchgate.net/publication/237052676_The_Epipelic_Diatom_Community_at_the_Downstream_Rivers_of_Torong_Galeh_and_Legi_Semarang_(Indonesia)] Alkalinity range is 90 to 200 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Diatoma bottnica&amp;#039;&amp;#039; may be 57 µm long and form attached zig-zag colonies in brackish water.[http://files.figshare.com/1704702/Table_S6.pdf]&lt;br /&gt;
&lt;br /&gt;
==Melosira==&lt;br /&gt;
[[Image:Melosira.jpg|thumb|left| Melosira sp. [http://www.savingwater.co.za/tag/melosira/]]]&lt;br /&gt;
[[Image:Aulacoseira_granulata.jpg|thumb|right| Aulacoseira granulata [http://planktonnet.awi.de/index.php?contenttype=image_details&amp;amp;itemid=16791#content © Barbara Meyer]]]&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus prefer to prey on the large size diatoms, such as Melosira granulata (&amp;#039;&amp;#039;Aulacoseira granulata&amp;#039;&amp;#039;).[http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] One single cell may be 22 to 37 µm long and 3.5 to 5 µm in diameter.[http://nordicmicroalgae.org/taxon/Aulacoseira%20granulata%20var.%20angustissima] Melosira granulata is often found in turbid water.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] Melosira sp. are meroplanktonic, meaning that they are part of plankton only during the larval stage of their life and spend their adult lives in between the plankton on the reef, which they may enter due to wind-induced mixing.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] Though Melosira granulata may also thrive in waters with relatively little nutrients [http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf], Melosira are considered to have high-nutrient requirements and may occur in completely mixed shallow eutrophic waters. Melosira sp in general are characteristic for low salinity and low alkalinity waters.[http://link.springer.com/chapter/10.1007/978-94-009-3095-7_3#page-1] Melosira granulata may thrive at 7 pH, 110 mg/L bicarbonate alkalinity and 260 mg/L hardness [http://www.researchgate.net/publication/256841993_OCCURRENCE_OF_PHYTOPLANKTON_IN_THE_WATER_BODIES_OF_MIRAJ_TAHASIL_OF_MAHARASHTRA/file/60b7d523e97e84f9aa.pdf] / at 7 to 8 pH [http://www.tandfonline.com/doi/abs/10.1080/00288330.1980.9515855#.VFkfo_mG_QM] / 7.2 to 8.5 pH (total bicarbonate alkalinity: 80 to 170 mg/L) [http://www.moef.nic.in/sites/default/files/nlcp/Indian%20Case%20Studies/Q-4.pdf] / 7.9 to 8.4 pH (Cl 64 mg/L ; NH&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; 0.28 mg/L ; P 0.23 mg/L) [http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1] / at 8 to 9 pH.[http://www.jstor.org/discover/10.2307/1662?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21105124407973] Melosira granulata is associated with freshwater lakes [http://books.google.nl/books?id=bz6UWRbwzHwC&amp;amp;dq=Diatoms+shallow+saline+alkaline+lakes&amp;amp;lr=&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s], but Melosira granulata may also be considered euryhaline (may thrive in a wide range of salinities).[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Melosira+granulata+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s][http://link.springer.com/article/10.1007/BF00025173#page-1] Melosira granulata is more abundant at higher temperatures. [http://www.sciencedirect.com/science/article/pii/S0272771409004636] Dominance of Melosira granulata requires [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae high levels of silicon] and high loadings of phosphorus and nutrient fluxes.[http://www.tandfonline.com/doi/abs/10.1080/0269249X.2003.9705580#.VIfvDjGG810] The alkalinity range may be 30 to 165 mg/L CaCo&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] Melosira granulata is less seasonal than offshore populations of Melosira victoriae and Melosira nyassensis.[http://link.springer.com/article/10.1007/BF00027237#page-1] &lt;br /&gt;
&lt;br /&gt;
Melosira / Aulacoseira (and cyclotella) species are Centrophycidae; centric diatoms as opposed to the other class: the pennate genera. The principal limiting resources of Melosira are light and phosphorus (and silica).[http://link.springer.com/chapter/10.1007/978-3-642-84077-7_20#page-1] &amp;#039;&amp;#039;Melosira distans&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Melosira ambigua&amp;#039;&amp;#039; need much light and have low phosphorus requirements and &amp;#039;&amp;#039;Melosira nyassensis&amp;#039;&amp;#039; needs the least light and the most phosphorus. Melosira granulata (which may adjust to light availability) and &amp;#039;&amp;#039;Melosira agassizii&amp;#039;&amp;#039; are intermediate.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] &amp;#039;&amp;#039;Melosira italica v. valida&amp;#039;&amp;#039; develops optimally in running water (reophilous).[http://pleistocenecoalition.com/vanlandingham/VanLandingham_2006.pdf] &amp;#039;&amp;#039;Melosira italica v. Italica&amp;#039;&amp;#039; is perennial and periphytic, associated with terrestrial habitats, but often suspended in the water.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&lt;br /&gt;
==Navicula==&lt;br /&gt;
[[Image:Anomoeoneis_sphaerophora.jpg|thumb|left| Anomoeoneis sphaerophora [http://s252.photobucket.com/user/Glenodin/media/Anomoeoneis-sphaerophora-2.jpg.html © Glenodin]]]&lt;br /&gt;
[[Image:Navicula_radiosa.jpg|thumb|right| Navicula radiosa [http://westerndiatoms.colorado.edu/taxa/species_image/navicula_radiosa/sem_fragment/4/4 © DUS]]]&lt;br /&gt;
&lt;br /&gt;
Navicula species may be preferentially selected by Oreochromis niloticus.[http://oceandocs.net/bitstream/1834/1160/1/9290640789-P93103.pdf][http://www.tandfonline.com/doi/abs/10.2989/16085914.2013.784698#.VGNeivmG811]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula radiosa&amp;#039;&amp;#039; (aka Schizonema radiosum) may be 40-120 μm long and 8-12 μm wide [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/navicula-radiosa-kutzing-1844], or 52-105 µm long and 8.8-11.2 µm wide.[http://westerndiatoms.colorado.edu/taxa/species/navicula_radiosa] It thrives at 21 to 27°C. It is often found in littoral waters and is indifferent of pH (&amp;gt;7 pH) and cationity.[http://content.lib.utah.edu/utils/getfile/collection/etd2/id/2138/filename/580.pdf] Navicula radiosa may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Navicula radiosa may attach to substrates, but not readily.[http://hrcak.srce.hr/file/5634]&lt;br /&gt;
&lt;br /&gt;
Most other navicula are small. Navicula species are considered to be planktonic; floating suspended in the water.[http://www.researchgate.net/publication/237052676_The_Epipelic_Diatom_Community_at_the_Downstream_Rivers_of_Torong_Galeh_and_Legi_Semarang_(Indonesia)]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula radiosa var. radiosa&amp;#039;&amp;#039; is 38-45 µm long, may be periphytic, current indifferent and confined to freshwater.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] &lt;br /&gt;
&amp;#039;&amp;#039;Navicula placentula&amp;#039;&amp;#039; (30-50 μm long and 12-20 μm [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/placoneis-placentula-ehrenberg-mereschkowsky-1903], or 23-28 µm long) may occur suspended in shallow waters.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
Navicula gottlandica var. gottlandica (36-44 µm long, 8-10 µm wide) may thrive in freshwater and brackish water. The temperature tolerance range is 0°-25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] &lt;br /&gt;
&amp;#039;&amp;#039;Navicula atomus, Navicula cari, Navicula cocconeiformis&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Navicula graciloides&amp;#039;&amp;#039; may grow in shallow lakes with 40 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula placentula f. rostrata&amp;#039;&amp;#039; is 37 µm long and 13.5 µm wide.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula arenaria&amp;#039;&amp;#039; may be 30-80 µm long [http://dbmuseblade.colorado.edu/DiatomTwo/dscb_site/species.php?g=Navicula&amp;amp;s=arenaria] and tolerate 8 ppt to 45 ppt salinity.[http://link.springer.com/article/10.1007/BF00395587#page-1] &lt;br /&gt;
&amp;#039;&amp;#039;Navicula halophila&amp;#039;&amp;#039; (aka Craticula halophila f. robusta, aka Navicula cuspidata var. halophila) may be 20-90 (140) µm long and 8-18 µm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/a-g/craticula-halophila-grunow-mann-in-round-crawford-mann-1990] Navicula halophila may grow in lakes with &amp;gt; 10 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;pg=PA203&amp;amp;lpg=PA203&amp;amp;dq=Navicula+halophila+salinity&amp;amp;source=bl&amp;amp;ots=EZrWpQjOfp&amp;amp;sig=fNBKrN4F547R3x4knyc1KTtTgp8&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=QjlqVLapOI3uaqzOgNgN&amp;amp;ved=0CCYQ6AEwAA#v=onepage&amp;amp;q=Navicula%20halophila%20salinity&amp;amp;f=false]&lt;br /&gt;
&amp;#039;&amp;#039;Navicula cryptocephala var. veneta&amp;#039;&amp;#039; (14-18 µm long and 5 µm wide) may occur over a broad range of salt concentrations in running water with pH over 7. It is periphytic or suspended in the (eutrophic) water. Never dominant. Range of tolerance is 0-25°C.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Navicula cryptocephala&amp;#039;&amp;#039; (20-40 μm long [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/navicula-cryptocephala-kutzing-1844], or 21-34 µm long, 5.2-6.5 µm wide[http://westerndiatoms.colorado.edu/taxa/species/navicula_cryptocephala]) may grow at 15 ppt salinity [http://link.springer.com/article/10.1007/BF00396925#page-1], needs pH over 7 and nitrogen in the form of ammonia or amino acids. It is eutrophic and common in both flowing and standing water. Navicula cryptocephala is perennial and may tolerate between 0 and 25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] It is favoured by high light penetration [https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] and is considered eurytopic (the ability to exist in a wide range of chemical environments) and may thrive at 0.15 to 4.6 mg/L nitrate, 7 to 65 mg/L chloride, 0.13 to 0.59 mg/L total phosphorus (as PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;) and 0.00 to 0.28 mg/L NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; [http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1], and in shallow water (&amp;lt;50 cm) at 1100 mg/L Na; 33 mg/L K; 8 mg/L Ca; 4 mg/L Mg; 3 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 45 SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 830 mg/L Cl and 26.5 meq/L alkalinity (carbonates plus bicarbonates).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Anomoeoneis sphaerophora&amp;#039;&amp;#039; (aka &amp;#039;&amp;#039;Navicula sphaerophora&amp;#039;&amp;#039;) may be 40-80 μm long [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/a-g/anomoeoneis-sphaerophora-ehrenberg-pfitzer-1871] or 25-200 µm long and 12-60 µm wide.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13070020.html] It is officially a benthic freshwater specie [http://www.algaebase.org/search/species/detail/?species_id=o2d66a17cc9854d7d&amp;amp;sk=0&amp;amp;from=results], but may be associated with Spirulina platensis dominance (and Nitzschia sigma) in highly alkaline, saline waters [http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] (8.3 - 10.6 pH) [http://books.google.nl/books?id=Rm08AAAAIAAJ&amp;amp;dq=Anomoeoneis+sphaerophora+spirulina+benthic&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]page280. Together with Nitzschia sp. 1 (12% of diatoms present) and Nitzschia sigma (8%) it may predominate (54%) in 18 ppt salinity, 284 mg/L potassium, 54 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 554 meq/L alkalinity, 120 mg/L phosphates and 9.8 pH. Together with Nitzschia sp. 1 (35% of diatoms present) and Nitzschia sigma (13%) it may dominate (43%) in 2 ppt salinity, 53 mg/L potassium, 10.6 mg/L calcium, 32 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 70 meq/L alkalinity, 11.2 mg/L phosphates and 10.3 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Anomoeoneis sphaerophora var. rostrata&amp;#039;&amp;#039; may dominate in &amp;lt;50 cm shallow waters at 9.6 pH and 25.5-130 meq/L (carbonates + bicarbonates) alkalinity (908-4810 mg/L Na; 22-45 mg/L K; 7-8 mg/L Ca; 1-6 mg/L Mg; 0.4 mg/l Fe; 0.7-3 SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 45-68 mg/L SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 674-2680 mg/L Cl).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Anomoeoneis sphaerophora var. sphaerophora&amp;#039;&amp;#039; is benthic and may dominate in 13 to 64 cm shallow fresh and brackish waters at pH 9.7 to 10.1 and 23.1ºC to 34.2ºC ; conductivity &amp;gt; 2870 µS/cm, and may also thrive in waters with lower pH (5.0-10.4) and lower conductivity (318-12070 µS/cm), and at 23.0-36.1°C [http://base.repositorio.unesp.br/bitstream/handle/11449/73904/2-s2.0-84875037147.pdf?sequence=1]&lt;br /&gt;
&lt;br /&gt;
==Nitzschia==&lt;br /&gt;
[[Image:Nitzschia_sp1.jpg|thumb|right| Nitzschia sp. 1 [http://dl.uncw.edu/digilib/biology/protists/taxonomy%20and%20systematics/meps_diatoms/site_05/st5_nitzschia_01.jpg]]]&lt;br /&gt;
[[Image:Nitzschia_accicularis.jpg|thumb|left| Nitzschia accicularis [http://www.serc.si.edu/labs/phytoplankton/guide/diatoms/nitzacic.aspx © SERC]]]&lt;br /&gt;
[[Image:Nitzschia_hungarica.jpg|thumb|right| Nitzschia hungarica [http://www.diatomloir.eu/Site%20Diatom/Saumatdeux.html]]]&lt;br /&gt;
[[Image:Nitzschia_sigma.jpg|thumb|left| Nitzschia sigma [http://www.mikroskopie-forum.de/index.php?topic=15303.0 © Frank Fox]]]&lt;br /&gt;
[[Image:Nitzschia_vermicularis_2.jpg|thumb|right| Nitzschia vermicularis [http://macroclub.ru/gallery/showphoto.php?photo=100656&amp;amp;cat=528 © A. Mikhaltsov]]]&lt;br /&gt;
[[Image:Nitzschia_sigmoidea.jpg|thumb|left| Nitzschia sigmoidea [http://www.diatomloir.eu/Site%20Diatom/Saumatdeux.html]]]&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus may prefer Spirulina and &amp;#039;&amp;#039;Nitzschia acicularis&amp;#039;&amp;#039; the most.[http://www.oceandocs.org/handle/1834/1160] Nitzschia acicularis (aka synedra acicularis) may be 30-50 µm long.[http://eprints.uni-kiel.de/644/] / 30-100 µm long and 3-4 µm wide [http://westerndiatoms.colorado.edu/taxa/species/nitzschia_acicularis] or 30-150 µm long and 2,2-5 µm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-acicularis-kutzing-smith-1853] Cell volume may be 280 μm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://link.springer.com/article/10.1007/s00442-004-1708-y#page-1] / may vary from 248 μm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; to 800 μm&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;[http://eol.org/pages/910724/overview]. Nitzschia acicularis is solitary motile in brackish / freshwater.[http://files.figshare.com/1704702/Table_S6.pdf] Nitzschia acicularis may tolerate high salinity.[http://www.akademiai.com/content/uph511t78463q663/] Alkalinity range is 80 to 220 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] Nitzschia acicularis may thrive in water with pH fluctuating from ~7 to 9.5 pH. (total P &amp;gt; 48 µg/L; nitrate-nitrogen &amp;gt; 80 µg/L)[http://www.maxwellsci.com/print/rjees/v4-511-524.pdf] Nitzschia acicularis may thrive in water with 8.3-8.6 pH, 4.5-7.0 DO, 125-309 total alkalinity, 70-220 mg/L Cl, 10-45 mg/L sulphate, 2-4 mg/L phosphate, 6.9-12.6 mg/L Mg, 7.5-10.5 mg/L Ca, 4.1-0.0 mg/L nitrate, 2.5-5.0 mg/L ammonium and 17-18.5ºC[http://www.jeb.co.in/journal_issues/200601_jan06/paper_06.pdf] Nitzschia acicularis may be found simultaneously in its planktonic and attached forms. Most intensive development of Nitzschia acicularis may primarily be in shallow waters in the river delta areas. It is typically found in rivers, ponds, and sometimes streams and bogs, but is very rarely found in lakes.[http://www.plankton.jp/PBE/issue/vol46_1/vol46_1_018.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia&amp;#039;&amp;#039; are Pennatophycidae (Bacillariophyta [http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf]), which are &amp;#039;&amp;#039;epipelic&amp;#039;&amp;#039; diatoms; benthic (=not free floating) diatoms growing on sediment.[http://www.scribd.com/doc/41415598/The-Epipelic-Diatom-Community-at-the-Downstream-Rivers-of-Torong-Galeh-and-Legi-Semarang-Indonesia] Upper ammonium tolerance of diatoms may be 3600 μM.[http://www.researchgate.net/publication/260240696_Acclimation_and_toxicity_of_high_ammonium_concentrations_to_unicellular_algae] Nitzschia species are not dominant at temperatures below 23°C.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] Many Nitzschia species stop growing at pH values of 8.7 to 9.1, and data suggest that smaller species have a higher upper pH limit for growth than larger species.[http://cat.inist.fr/?aModele=afficheN&amp;amp;cpsidt=15924847] Most Nitzschia may survive in 10 pH.[http://ofmpub.epa.gov/storpubl/storet_wme_pkg.Display_Station?p_station_id=REFRC2&amp;amp;p_org_id=MDEQ_WQ_WQX] Nitzschia are intermediates along the silicon:phosphorus gradient; with relatively balanced silicon and phosphorus requirements.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] Nitzschia species do very [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae poorly in low SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;] waters.[[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia sigma&amp;#039;&amp;#039; may be 388 µm long [http://files.figshare.com/1704702/Table_S6.pdf] 35-1000 µm long [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf] / 30-200 µm long and 4-13 µm wide [http://westerndiatoms.colorado.edu/taxa/species/nitzschia_sigma1], or 40-180 µm long and 4-8 µm wide[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-sigma-kutzing-smith-1853], is periphytic, thrives at pH &amp;gt;7, and may occur over a wide range of salt concentrations. [http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] It is associated with fresh- [http://files.figshare.com/1704702/Table_S6.pdf], saline, alkaline [http://qspace.library.queensu.ca/handle/1974/500], and even hypersaline waters.[http://link.springer.com/chapter/10.1007/978-3-642-70290-7_17#page-1] It may tolerate up to 45 ppt salinity.[http://link.springer.com/article/10.1007/BF00395587#page-1] Nitzschia sigma is capable of free movement [http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.1950.tb07503.x/abstract], benthic and typically found in estuarine (brackish) waters. It has its optimum at 25°C or higher.[http://link.springer.com/article/10.1007/BF00395586#page-1] It is tolerant of pollution. [http://link.springer.com/article/10.1007/PL00012144#page-1] Nitzschia sigma grows well when exposed to 380–450 μM water ammonium concentrations.[http://www.tandfonline.com/doi/abs/10.1080/09670269810001736673#.VGizfvmG810]&lt;br /&gt;
* Together with Nitzschia sp. 1 (35% of diatoms present) and Amonoeoneis sphaerophora (43%) it may grow well (13%) in 2 ppt salinity, 53 mg/L potassium, 10.6 mg/L calcium, 32 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 70 meq/L alkalinity, 11.2 mg/L phosphates and 10.3 pH.&lt;br /&gt;
* Together with Nitzschia sp. 1 (12% of diatoms present) and Amonoeoneis sphaerophora (54%) it may grow well (8%) in 18 ppt salinity, 284 mg/L potassium, 54 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 554 meq/L alkalinity, 120 mg/L phosphates and 9.8 pH.&lt;br /&gt;
* It may dominate (48% of diatoms present) in 5.8 ppt salinity, 69 mg/L potassium, 5.7 mg/L calcium, 34 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 163 meq/L alkalinity, 15.5 mg/L phosphates and at 10.5 pH. &lt;br /&gt;
* It may dominate (45% of diatoms present) in 1.9 ppt salinity, 54 mg/L potassium, 12 mg/L calcium, 28 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 30 meq/L alkalinity, 5.2 mg/L phosphates and at 10.5 pH.&lt;br /&gt;
* It may dominate (41% of diatoms present) in 3.8 ppt salinity, 284 mg/L potassium, 177 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 107 meq/L alkalinity, 9.2 mg/L phosphates and at 9.4 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia sigmoidea&amp;#039;&amp;#039; may attach to substrates [http://hrcak.srce.hr/file/5634], be motile, suspended in brackish water or freshwater and may be 314 µm [http://files.figshare.com/1704702/Table_S6.pdf] 90-500 µm [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf] / 75-250 µm [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf] / 282-350 µm long and 12,5-26 µm wide. It may be periphytic, eutrophic, current indifferent, alkaliphilous and characteristic of the zone where oxidation of biodegradable compounds is complete. It is perennial and tolerates 0 to 25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia sp. 1&amp;#039;&amp;#039; may attach to substrates [http://hrcak.srce.hr/file/5634] and concentrations occur when input water has [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae high Si:P ratios] (warm, wet climate).[http://qspace.library.queensu.ca/handle/1974/500] Nitzschia sp. 1 may be 179 µm long and 6 µm wide.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] Nitzschia sp. 1 (only 85 µm long) is benthonic and only dominant in extremely shallow (saline, alkaline) waters.[http://www.avto.aslo.info/lo/toc/vol_18/issue_1/0053.pdf] Similar to Amonoeoneis sphaerophora and Nitzschia sigma, it may do very well (35% of diatoms present) in 2 ppt salinity, 53 mg/L potassium, 10.6 mg/L calcium, 32 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;,70 meq/L alkalinity, 11.2 mg/L phosphates and 10.3 pH. Equally so, it may also grow well (12% of diatoms present) in 18 ppt salinity, 284 mg/L potassium, 54 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 554 meq/L alkalinity, 120 mg/L phosphates and 9.8 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia hungarica&amp;#039;&amp;#039; (aka Tryblionella hungarica; 35-130 µm long [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-hungarica-grunow-1862]) is benthonic and only dominant in extremely shallow (saline, alkaline) waters.[http://www.avto.aslo.info/lo/toc/vol_18/issue_1/0053.pdf] Nitzschia hungarica is motile, current indifferent, epilythic (growing on sediment), thrives in brackish (/ fresh[http://files.figshare.com/1704702/Table_S6.pdf]) and running water with pH &amp;gt;7.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] It may do well (29% of diatoms present) in 3.7 ppt salinity, 174 mg/L potassium, 8.7 calcium, 1500 mg/L sulphates, 11.8 mg/L SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;, 87.4 meq/L alkalinity and 19 mg/L phosphates and 9.0 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia vermicularis&amp;#039;&amp;#039; may be motile in brackish waters and may be 220 µm long and 18 µm wide[http://macroclub.ru/gallery/showphoto.php?photo=100656&amp;amp;cat=528], 75-250 µm [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf], 170 µm [http://files.figshare.com/1704702/Table_S6.pdf] or 140 µm long, and 6 µm wide. It is periphytic, pH indifferent, current indifferent, and thrives in fresh water, tolerating small amounts of salt. It is characteristic of the zone where oxidation of biodegradable compounds is complete.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia palea&amp;#039;&amp;#039; (aka Synedra palea; 12-42 µm long[http://westerndiatoms.colorado.edu/taxa/species/nitzschia_palea], or 15-70 µm long and 2,5-5 µm wide[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-palea-kutzing-smith-1856], or 19-53 µm long, 3.8-5 µm wide) may be the most resistant and most tolerant of all diatoms. It is solitary motile [http://files.figshare.com/1704702/Table_S6.pdf], eurytopic (indifferent of chemical conditions), euryhaline (indifferent of salinity) and eurythermic (indifferent of temperature) and grows in polluted water (but containing moderate dissolved oxygen) forming a brown surface layer on rocks of rapids, as well as in quiet water on shallow silt banks.[http://archive.org/stream/ecologyofalgaesy00tryo/ecologyofalgaesy00tryo_djvu.txt] Nitzschia palea may occur in shallow waters [https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] at 43 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] Domination is reed-associated. Nitzschia palea may dominate in &amp;lt;50 cm shallow waters at 9.6 pH and 25.5-130 meq/L (carbonates + bicarbonates) alkalinity (908-4810 mg/L Na; 22-45 mg/L K; 7-8 mg/L Ca; 1-6 mg/L Mg; 0.4 mg/l Fe; 0.7-3 SiO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;; 45-68 mg/L SO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;; 674-2680 mg/L Cl).[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
Nitzschia palea may dominate in 13 to 44 cm shallow fresh water at pH 7.0 to 8.5, and at 23 to 32°C (Optimum range: 22.8-29.5°C); conductivity 430-&lt;br /&gt;
2110 µS/cm.[http://base.repositorio.unesp.br/bitstream/handle/11449/73904/2-s2.0-84875037147.pdf?sequence=1]&lt;br /&gt;
Nitzschia palea has its maximum rate of photosynthesis at 33°C and is strongly inhibited at 40°C.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] &lt;br /&gt;
Nitzschia palea is considered eurytopic (the ability to exist in a wide range of chemical environments) and may thrive at 0.15 to 4.6 mg/L nitrate, 7 to 65 mg/L chloride, 0.13 to 0.59 mg/L total phosphorus (as PO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;&amp;lt;sup&amp;gt;-&amp;lt;/sup&amp;gt;) and 0.00 to 0.28 mg/L NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;. [http://kb.osu.edu/dspace/bitstream/handle/1811/22696/V080N3_108.pdf;jsessionid=ACE618356C8ED9A8B0B25FD9C9629D10?sequence=1]&lt;br /&gt;
Nitzschia palea is eutrophic, pH indifferent, current indifferent and may be suspended in the water or attached to substrates.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia frustulum&amp;#039;&amp;#039; (18 µm long) is an estuarine, benthic diatom [http://onlinelibrary.wiley.com/doi/10.1046/j.1529-8817.2000.99148.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] that is solitary motile [http://files.figshare.com/1704702/Table_S6.pdf] and develops optimally in stagnant waters (limnophilous).[http://www.jstor.org/discover/10.2307/2433656?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21105171827623] Nitzschia frustulum (aka Synedra minutissima / perpusilla / quadrangula) is both benthonic and associated with Spirulina platensis blooms. Nitzschia frustulum is a nitrogen heterotroph and dense populations of Spirulina platensis release organic nitrogen. In the presence of Spirulina platensis (so that the diatom can enter the plankton), Nitzschia frustulum is the dominant diatom in saline lakes with alkalinity 80 &amp;gt; mEq/L, but also survives in freshwater.[http://qspace.library.queensu.ca/handle/1974/500] Nitzschia frustulum may grow in waters with 3 to 100 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] It may be predominant in the range of 3.9 to 4.8 ppt salinity, 164-188 meq/L alkalinity and 10.1-10.4 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia frustulum var. frustulum&amp;#039;&amp;#039; (aka Synedra frustulum) may be 5-60 μm long and 2-4,5 μm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/nitzschia-frustulum-var.-frustulum-kutzing-grunow-in-cleve-grunow-1880]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia americana&amp;#039;&amp;#039; grows optimally at 26 ppt salinity.[http://plankt.oxfordjournals.org/content/8/1/215.abstract] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia archibaldii&amp;#039;&amp;#039; may thrive at 7.1 to 8.2 pH, 0 to 15 mg/L carbonates plus 110 to 170 mg/L bicarbonates alkalinity and 188 to 260 mg/L hardness.[http://www.researchgate.net/publication/256841993_OCCURRENCE_OF_PHYTOPLANKTON_IN_THE_WATER_BODIES_OF_MIRAJ_TAHASIL_OF_MAHARASHTRA/file/60b7d523e97e84f9aa.pdf] It may be 23 µm long and grow in marine / brackish water. It is solitary motile.[http://files.figshare.com/1704702/Table_S6.pdf]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia bolivianais&amp;#039;&amp;#039; is epilithic; growing on rocks.[http://www.bioone.org/doi/abs/10.1635/0097-3157(2007)156%5B123:EDBFCF%5D2.0.CO%3B2]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia closterium&amp;#039;&amp;#039; exhibits optimum photosynthetic activity at 10 ppt [http://link.springer.com/article/10.1007/BF00346767#page-1], but it may tolerate salinity over 35‰ (ppt)[http://m.m.aslo.info/lo/toc/vol_8/issue_2/0263.pdf], even up to 100 ppt.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia denticulata&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Nitzschia dissipata&amp;#039;&amp;#039; (solitary motile[http://files.figshare.com/1704702/Table_S6.pdf], 11.9-72 µm long[http://westerndiatoms.colorado.edu/taxa/species/nitzschia_dissipata]) in particular (and Nitzschia accicularis to a lesser extent) may thrive at 7.2 to 8.5 pH (total bicarbonate alkalinity: 80 to 170 mg/L).[http://www.moef.nic.in/sites/default/files/nlcp/Indian%20Case%20Studies/Q-4.pdf] Nitzschia dissipata is benthic and typically found in estuarine (brackish) waters. It has its optimum at 25°C or higher.[http://link.springer.com/article/10.1007/BF00395586#page-1]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia fonticola&amp;#039;&amp;#039; (10-55 µm long [http://westerndiatoms.colorado.edu/taxa/species/nitzschia_fonticola] or 18-31 µm long) is euryhaline and may thrive in 7 to 50 ppt.[http://www.cambridge.org/nl/academic/subjects/life-sciences/plant-science/diatoms-applications-environmental-and-earth-sciences?format=PB] Unlike most diatoms, Nitzschia fonticola is a nitrogen heterotroph (requiring organic nitrogen).[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia granulata&amp;#039;&amp;#039; may grow in shallow waters at 43 ppt salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia intermedia&amp;#039;&amp;#039; is solitary motile, thrives in brackish or freshwater and may be 80 µm [http://files.figshare.com/1704702/Table_S6.pdf] or 132 µm long and 7 µm wide.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&amp;#039;&amp;#039;Nltzschia linearis&amp;#039;&amp;#039; (related to Nitzschia palea) is truly rheobiontic (especially found in running water).[http://archive.org/stream/ecologyofalgaesy00tryo/ecologyofalgaesy00tryo_djvu.txt], 34-228 µm long [http://journals.tubitak.gov.tr/botany/issues/bot-06-30-5/bot-30-5-4-0512-4.pdf], 60-150 µm long and 4-6 µm wide[http://westerndiatoms.colorado.edu/taxa/species/nitzschia_linearis], or 106-157 µm long and 5.4-6.5 µm wide, periphytic, eutrophic, and thrives in running freshwater (at 7 pH or above) but able to tolerate small amounts of salt (&amp;lt; 500 mg/L). Tolerates 0 to 30°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd]&lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia ovalis&amp;#039;&amp;#039; may grow at salinities from 5–40‰ (ppt).[http://link.springer.com/article/10.1007%2FBF00397604#page-1]  &lt;br /&gt;
&amp;#039;&amp;#039;Nitzschia vitrea&amp;#039;&amp;#039; may grow in 3 to 100 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
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Some (&amp;#039;&amp;#039;Pseudo&amp;#039;&amp;#039;)Nitzschia species produce domoic acid, a neurotoxin that may cause amnesic shellfish poisoning in humans. Nitzschia pungens [http://www.nrcresearchpress.com/doi/abs/10.1139/f89-156#.VFpLUvmG_QM], Nitzschia pseudodelicatissima [http://www.int-res.com/articles/meps/67/m067p177.pdf]) and particularly Pseudo-nitzschia species in high pH conditions [http://cat.inist.fr/?aModele=afficheN&amp;amp;cpsidt=15924847], such as the cosmopolites Pseudo-nitzschiaseriata delicatissima and Pseudo-nitzschiaseriata pseudodelicatissima, but most notably the cosmopolites Pseudo-nitzschiaseriata australis and Pseudo-nitzschiaseriata multiseries [http://www.sciencedirect.com/science/article/pii/S1568988302000148] may produce domoic acid in the presence of light.[http://www.nrcresearchpress.com/doi/abs/10.1139/f91-137#.VFpO6PmG_QM] Pseudo-Nitzschia may tolerate up to 45 ppt salinity.[http://www.academia.edu/211282/Effects_of_salinity_on_Pseudo-nitzschia_species_Bacillariophyceae_growth_and_distribution]&lt;br /&gt;
&lt;br /&gt;
==Rhopalodia==&lt;br /&gt;
[[Image:Rhopalodia_gibba.jpg|thumb|left| Rhopalodia gibba [http://www.diatomloir.eu/Diatodouces/Canalraphe.html © diatomloir.eu]]]&lt;br /&gt;
[[Image:Rhopalodia_vermicularis.jpg|thumb|right| Rhopalodia vermicularis [http://www.lenaturaliste.net/forum/viewtopic.php?f=90&amp;amp;t=14237&amp;amp;p=77151 © Jean Peynichou]]]&lt;br /&gt;
&lt;br /&gt;
Rhopalodia spp. are littoral diatoms, thriving in brackish waters.[http://link.springer.com/chapter/10.1007/978-3-642-79066-9_15#page-1] Rhopalodia sp are epipelic algae; growing on sediment.[http://www.academia.edu/5264297/QUALITATIVE_AND_QUANTITATIVE_STUDY_OF_EPIPELIC_ALGAE_AND_RELATED_ENIVERNOMENTAL_PARAMETERS_IN_AL-HILLA_RIVER_IRAQ] Rhopalodia is endemic to East Africa [http://books.google.nl/books?id=aCjIB5YRtnkC&amp;amp;dq=Rhopalodia+vermicularis&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]. &lt;br /&gt;
&lt;br /&gt;
Despite their low abundance in pond water, both wild and pond-cultured Oreochromis niloticus preferentially selected &amp;#039;&amp;#039;Rhopalodia vermicularis&amp;#039;&amp;#039;.[http://www.tandfonline.com/doi/abs/10.2989/16085914.2013.784698#.VFu___mG_QM] Rhopalodia vermicularis may be 125 μm long and 14 μm wide. [http://planktonnet.awi.de/index.php?contenttype=image_details&amp;amp;itemid=60625#content] and dominate in shallow waters.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf] Rhopalodia vermicularis thrives in moderately saline and moderately alkaline, shallow waters. [http://in-africa.org/wp-content/uploads/2012/12/Owen-et-al-1982-Nature-Holocene-L-Turkana1.pdf]. Rhopalodia vermicularis grows in fresh to hyperalkaline lakes; 7 to 9.5 pH and 10 to 100 meq/L alkalinity (sodium bicarbonate or calcium- plus magnesium bicarbonate), at 21 to 27°C. [http://content.lib.utah.edu/utils/getfile/collection/etd2/id/2138/filename/580.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Rhopalodia gibba&amp;#039;&amp;#039; (aka Navicula gibba) may be 22-300 μm long and 18-30 μm wide [http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/h-z/rhopalodia-gibba-ehrenberg-muller-1895], 75-205 µm long and 8-11 µm wide.[http://westerndiatoms.colorado.edu/taxa/species/rhopalodia_gibba], grow at pH 10 and low alkalinity; 9.[http://ofmpub.epa.gov/storpubl/storet_wme_pkg.Display_Station?p_station_id=REFRC2&amp;amp;p_org_id=MDEQ_WQ_WQX] Rhopalodia is epiphytic [http://westerndiatoms.colorado.edu/taxa/species/rhopalodia_gibba], periphytic, solitary, and usually occurs in high pH fresh- or brackish water.[http://craticula.ncl.ac.uk/EADiatomKey/html/taxon13690050.html] Rhopalodia gibba may occur in alkaline freshwater (&amp;lt; 500 mg salt/L), is eutrophic and may tolerate heavy pollution with nitrogen in the form of amino acids or ammonia. It is both periphytic and suspended in water. It occurs in lakes, rivers and streams and is current indifferent.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] Rhopalodia gibba may also grow in 20 to 50 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] Rhopalodia gibba thrives in shallow water, positively correlating with total phosphates.[http://botanika.bf.jcu.cz/thesis/pdf/Mares%20_bc06.pdf] Nitrogen limitation may favor Rhopalodia gibba relative to other diatoms, presumably due to the ability of their cyanobacterial endosymbionts to fix atmospheric nitrogen. As ambient nitrogen levels decrease, the number of endosymbionts increases, but only in the presence of sufficient phosphorus. [http://nau.edu/Centers-Institutes/Ecoss/_Forms/Publications/deyoe-lowe-and-marks-1992/] Together with Synedra ulna, it may grow in 1.2 ppt salinity, 10 mg/L potassium, 56.4 mg/L calcium, 21 mg/L SiO2, 5.7 meq/L alkalinity, 1.9 mg/L phosphates and 8.6 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Rhopalodia gibberula&amp;#039;&amp;#039; (aka Epithemia gibberula / Eunotia gibberula) may be 25-80 µm long and 7-14 µm wide [http://dbmuseblade.colorado.edu/DiatomTwo/dscb_site/species.php?g=Rhopalodia&amp;amp;s=gibberula]), may grow in marine and freshwater [http://www.marbef.org/data/aphia.php?p=taxdetails&amp;amp;id=149568] and may dominate in hypersaline waters.[http://link.springer.com/chapter/10.1007/978-3-642-70290-7_17#page-1] Rhopalodia gibberula may thrive in 3 to 50 ppt salinity.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Navicula+halophila+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Rhopalodia gracilis&amp;#039;&amp;#039; may dominate in shallow waters.[https://qmro.qmul.ac.uk/jspui/bitstream/123456789/1619/1/OWENQuaternaryDiatomaceous1981.pdf]&lt;br /&gt;
&lt;br /&gt;
==Synedra Ulna==&lt;br /&gt;
[[Image:Synedra_ulna.jpg|thumb|right| Synedra ulna [http://www.diatomloir.eu/Diatodouces/diatomun/Synedra%20ulna.jpg © diatomloir.eu]]]&lt;br /&gt;
Oreochromis niloticus prefer to prey on the large size diatoms, such as &amp;#039;&amp;#039;Syndra ulna&amp;#039;&amp;#039; [http://www.academia.edu/5470071/DEMONSTRATION_OF_THE_PREDATION_EFFECT_OF_OREOCHROMIS_NILOTICUS_ON_THE_WATER_QUALITY_AND_SPECIES_COMPOSITION_OF_PLANKTON_ASSEMBLAGES] (aka Fragilaria ulna). It may be 27-600 μm long and 3,5-9 μm wide.[http://www.isprambiente.gov.it/it/banche-dati/acque-interne-e-marino-costiere-1/atlante-delle-diatomee-bentoniche-dei-corsi-dacqua-italiani/specie/a-g/fragilaria-ulna-nitzsch-lange-bertalot-1980]&lt;br /&gt;
&amp;#039;&amp;#039;Synedra ulna&amp;#039;&amp;#039; may be considered a benthic freshwater diatom that thrives in shallow estuarine environments close to freshwater sources. [http://books.google.nl/books?id=HrNwdRj1ehoC&amp;amp;dq=Synedra+ulna+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] Synedra ulna readily attaches to substrates by mucilage stalks or pads.[http://hrcak.srce.hr/file/5634] It may form a bushy, attached colony in brackish / freshwater [http://files.figshare.com/1704702/Table_S6.pdf], in shallow, saline, turbid water [http://www.schweizerbart.de/papers/algol_stud/detail/123/74435/Phytoplankton_seasonality_of_a_shallow_turbid_lake], or in shallow, oligotrophic lakes,[http://www.imp-du.com/downloadRadovi/Jasprica_Hafner%20-%20diatoms.pdf].&lt;br /&gt;
Synedra ulna is benthic (rocks, stones, plants) and may also be suspended in the water. It prefers dirty, flowing water with high nitrate, and may be most abundant at 22 to 39°C (optimum 25°C). It may tolerate 5.7-9.0 pH (optimum 6.4-8.3 pH).[http://books.google.nl/books?id=HrNwdRj1ehoC&amp;amp;dq=Synedra+ulna+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s](page 877)&lt;br /&gt;
Synedra ulna is perennial, suspended in the water, current indifferent, able to tolerate small amounts of salt and develops optimally at ph &amp;gt;7. It is eutrophic, characteristic of the zone where oxidation of biodegradable compounds is complete, or oxidation is still occurring. It develops over the range of 0 to 25°C.[http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=9004&amp;amp;context=rtd] Synedra ulna may dominate in saline, alkaline waters.[http://books.google.nl/books?id=NOdvPFm6SyoC&amp;amp;dq=Synedra+ulna+salinity&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] Alkalinity range is 37 to 170 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;.[http://www.limnetica.com/Limnetica/Limne24/L24a133_diatoms_spanish_reservoirs.pdf] Synedra ulna may thrive in water with 7.0-8.6 pH, 1.0-7.0 DO, 125-550 total alkalinity, 70-247 mg/L Cl, 10-60 mg/L sulphate, 2-5.6 mg/L phosphate, 6.9-23.5 mg/L Mg, 7.5-27.5 mg/L Ca, 1.6-9.0 mg/L nitrate, 2.5-10.2 mg/L ammonium and 17-34.5ºC[http://www.jeb.co.in/journal_issues/200601_jan06/paper_06.pdf] Synedra ulna has [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae high silicon] and low phosphorus requirements.[http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf] Together with Rhopalodia gibba, it may grow in 1.2 ppt salinity, 10 mg/L potassium, 56.4 mg/L calcium, 21 mg/L SiO2, 5.7 meq/L alkalinity, 1.9 mg/L phosphates and 8.6 pH.[http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf]&lt;br /&gt;
&lt;br /&gt;
==Anabaena==&lt;br /&gt;
[[Image:Anabaena_cylindrica.jpg|thumb|left| Anabaena cylindrica [http://cyanobacteria.myspecies.info/sites/cyanobacteria.myspecies.info/files/Anabaena_cf._cylindrica_AUTH_0699_63x%20%2820%29.jpg © snkonsta]]]&lt;br /&gt;
Anabaena sp. are large filamentous and neurotoxin-producing cyanobacteria (blue-green algae) that exist as plankton.&lt;br /&gt;
Feeding mostly on Anabaena spiroides, Oreochromis niloticus (mean total weight: 31.5 g) may ingest 5.1% body mass equivalent (wet weight basis).[http://repository.seafdec.org.ph/handle/10862/1848]&lt;br /&gt;
Ingestion rates are higher on Anabaena cylindrica than on Microcystis aeruginosa.[http://link.springer.com/article/10.1007/BF00002160#page-1] Anabaena flos-aquae is much better assimilated (83%) than green algae. Gross growth efficiency of fish fed Anabaena flos-aquae was 46% in comparison to fish fed Chlamydomonas (22%) and Ankistrodesmus falcatus; 24%.[http://link.springer.com/article/10.1007/BF00379861#page-1] Tilapia grow much better on Anabaena than on Clamydomonas (green algae) [http://link.springer.com/article/10.1007/BF00000362#page-1] Nile Tilapia fed ration equal 3% of the total fish body weight, containing 1.5% Anabaena had higher growth and were relatively protected against A. hyrophila infection.[http://www.scopemed.org/?jft=31&amp;amp;ft=31-1378827209] Anabaena circinalis may cause off-flavor in fish flesh.[http://www.cabdirect.org/abstracts/20133297816.html] In competition experiments between Anabaeana and toxic Microcystis aeruginosa, dominancy is mainly determined by the initial biomass ratio.[http://www.researchgate.net/publication/236246459_Competition_between_toxic_Microcystis_aeruginosa_and_nontoxic_Microcystis_wesenbergii_with_Anabaena_PCC7120] Anabaena circinalis and Anabaena spiroides thrive in salinity less than 3 ppt.[http://researchonline.nd.edu.au/era_sci__article/3/] Anabaena tortulosa is relatively tolerant of salinity up to 15 ppt.[http://www.cdsewer.org/BdAct/Presentations/USU_Bioassay_Report_DRAFT_4B.pdf] Anabaena spp. (and Anabaenopsis) are common in alkaline saline lakes. [http://www.researchgate.net/publication/6971813_Toxic_cyanobacteria_and_their_toxins_in_standing_waters_of_Kenya_implications_for_water_resource_use] Salt tolerance in cyanobacteria is increased by nitrogenous compounds (inhibiting Na&amp;lt;sup&amp;gt;+&amp;lt;/sup&amp;gt; influx), but by nitrate more so than by ammonium or glutamine.[http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&amp;amp;action=reflink&amp;amp;origin=wiley&amp;amp;version=1%2E0&amp;amp;coi=1%3ACAS%3A528%3ADyaL28Xjt1an&amp;amp;md5=25a98907beddc7831f788b4c5b39f7bf]&lt;br /&gt;
&lt;br /&gt;
==Spirulina==&lt;br /&gt;
[[Image:Spirulina.jpg|thumb|left| Spirulina platensis [http://naturalactives.com/micro-algae-fights-skin-ageing/]]]&lt;br /&gt;
[[Image:Spirulina_3.jpg|thumb|right| Arthrospira platensis [http://www.sbs.utexas.edu/utex/algaeDetail.aspx?algaeID=4383 © UT-Austin]]]&lt;br /&gt;
[[Image:Spirulina_2.jpg|thumb|left| Spirulina platensis [http://imworld.ru/priroda/spirulina-samoe-poleznoe-rastenie-na-zemle.html]]]&lt;br /&gt;
[[Image:Spirulina_4.jpg|thumb|right| Arthrospira platensis [http://fmp.conncoll.edu/Silicasecchidisk/LucidKeys/Carolina_Key/html/Arthrospira_Main.html]]]&lt;br /&gt;
&lt;br /&gt;
Spirulina (Oscillatoria, Arthrospira) are blue-green algae/cyanobacteria and a rich source of protein and potassium.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.2003.tb09615.x/abstract] Spirulina platensis contains 50 to 65% protein (10% is non-protein nitrogen)[http://pubs.acs.org/doi/abs/10.1021/jf00105a022], is high in vitamin B1 and B6 and is 85 to 95% assimilated. It may contain up to 14% lipids (of dry mass). [http://link.springer.com/article/10.1023%2FB%3ACONC.0000039141.98247.e8#page-1] Spirulina platensis growth is optimum below 10.5 pH.[http://www.sciencedirect.com/science/article/pii/S0044848603001236] Spirulina platensis is naturally found in tropical regions inhabiting alkaline lakes (pH 9.4 to 11 ; HCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; + CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; &amp;gt; 51 meq/L [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283708/?page=5]) with high concentration of NaCl and bicarbonates [http://www.scielo.br/scielo.php?pid=S1517-83822008000100022&amp;amp;script=sci_arttext&amp;amp;tlng=pt], but may adapt to very different habitats, including freshwater.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283708/?page=2] In highly alkaline conditions (&amp;gt;50 meq/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity; 9.8-11 pH), at higher salt levels (2.5 to 30 g. salt / L; mostly carbonates and bicarbonates), Spirulina platensis becomes predominant (with Anabaenopsis, Oscillatoria, Synechocystis), or even the only significant organism (&amp;gt; 30 g. salt / L ; 10.5 &amp;lt; pH &amp;gt; 11) Optimum salinity may be between 20 and 70 ppt. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283708/?page=3] (seawater salinity is 35 ppt) Oreochromis niloticus may fairly tolerate salinity up to 10 ppt [http://www.sciencedirect.com/science/article/pii/0044848685901024] Tilapia may tolerate 0.5% salinity (5 ppt).[http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf] &lt;br /&gt;
At 8 g / L salinity adult Oreochromis niloticus may be stocked at 40 fish / m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;. At 15 g/L salinity total production is more than halved.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2006.01605.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
Oreochromis niloticus may also (barely) survive in lakes with ~27‰ salinity.[http://sites.wetlands.org/reports/ris/1EG003_RIS.pdf] Oreochromis niloticus need 7.4-8.2 pH; 50-140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity and 140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;/L hardness.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
Spirulina platensis (small biomass) may be grown on [http://www.waiwiki.org/index.php?title=Composting_toilet#Urine human urine].[http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L [http://link.springer.com/article/10.1007%2FBF02179776#page-1], the best growth temperature being 29°C [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] at an appropriate slowly increasing urea feeding rate.[http://www.sciencedirect.com/science/article/pii/S0044848604005952] &lt;br /&gt;
Regarding phosphorus, maximum biomass production at high light intensity was observed at 250 mg/L K&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;HPO&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt;.[http://link.springer.com/article/10.1007/s11274-012-1076-4#page-1] The optimum nitrogen:phosphorus ratio is 7:1 at optimum light intensity.[http://link.springer.com/article/10.1007/BF00016689#page-1] The temperature range for optimum Spirulina growth is 30 to 35°C.[http://link.springer.com/article/10.1023/A:1009233230706#page-1] At 35°C there is a negative effect on biomass production but a positive effect on the production of protein, lipids and phenolics.[http://www.sciencedirect.com/science/article/pii/S0960852405004761] Spirulina may be cultivated in water with 16 g/L baking soda, 2 g/L Potassium nitrate, 1 g/L sea salt, 0.1 g/L potassium phosphate and 0.01 g/L iron sulphate. [http://en.wikipedia.org/wiki/Spirulina_(dietary_supplement)] In an open raceway system dissolved oxygen may reach 30 mg/L at 28°C. Above 25 mg/L Spirulina growth is inhibited.[http://www.sciencedirect.com/science/article/pii/S0044848603001236] During a period of nitrogen starvation, Spirulina platensis may survive and grow unaffected based on/due to prior storage of nitrogen in pigment proteins (C-Phycocyanin).[http://link.springer.com/article/10.1007/BF00403211#page-1]&lt;br /&gt;
&lt;br /&gt;
Tilapia fed solely on raw Spirulina keep normal reproduction throughout three generations.[http://www.sciencedirect.com/science/article/pii/S0044848603008226]&lt;br /&gt;
Larval tilapia that were fed solely raw Spirulina at a feeding rate of 30% (on a dry basis) of bodyweight in the first 3 weeks, 10% in weeks 4–6, and 3% in weeks 7–10, kept growing without any abnormality[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00388.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false], at a slightly lower rate than fish fed fishmeal. Fish fed Spirulina had higher protein, polar lipid, linoleic acid and γ-linolenic acid contents and lower ash and omega-3 contents than fish fed the commercial diet.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2002.00386.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Elastic modulus of flesh of the Spirulina-fed fish was significantly higher and viscosity lower.[http://onlinelibrary.wiley.com/doi/10.1046/j.1444-2906.2003.00653.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Larval Tilapia (Oreochromis niloticus &amp;lt; 3.4 cm) prefer consuming Spirulina platensis over Euglena gracilis (flagellate protists), and is also more readily assimilated. Tilapia prefer both species over Chlorella vulgaris (green algae, 45% dry matter protein), which is hardly ingested by larval tilapia.[http://www.sciencedirect.com/science/article/pii/S0044848604002595] Tilapia prefer Spirulina over Navicula (a diatom).[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA465&amp;amp;dq=algae+tilapia&amp;amp;ots=BVjAbOF-zV&amp;amp;sig=aEIMIY5ekRp5adhtpwsOIqPQTMQ#v=onepage&amp;amp;q=algae%20tilapia&amp;amp;f=false] In Tilapia aurea fed Spirulina platensis, food conversion was 2.0.[http://www.sciencedirect.com/science/article/pii/004484867690140X] Dietary Spirulina incorporation increases antioxidant activity in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25231739] When live Spirulina partly replaces commercial feed, fish mortality due to Aeromonas hydrophila infection decreases with an increase in the Spirulina level in the diet.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2009.02195.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
==Microcystis==&lt;br /&gt;
In lakes where Microcystis spp. are the most abundant, Microcystis sp. may account for more than 80% of ingested phytoplankton.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Various cyanobacteria may produce toxins (eg Microcystis aeruginosa). Average digestion rate of Microcystis aeruginosa (cyanobacteria producing neurotoxins and hepatotoxins) by Tilapia fingerlings is 58 to 78% at 25℃.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-SCKX200506012.htm] In Oreochromis niloticus a high rate of defaecation of undigested Microcystis may occur.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1998.tb00104.x/abstract]&lt;br /&gt;
&lt;br /&gt;
Microcystis aeruginosa may survive in a wide range of salinities.[http://link.springer.com/chapter/10.1007/978-94-009-3095-7_3#page-1] Oreochromis mossambicus exposed to extracts from Microcystis aeruginosa showed significant plasma hypocalcaemia.[http://jeb.biologists.org/content/199/6/1319.short] Chronic exposure to toxin producing cyanobacteria (Microcystis aeruginosa) may not (115 days trial[http://www.sciencedirect.com/science/article/pii/S0041010109001925], 90 days trial[http://www.sciencedirect.com/science/article/pii/S004484860600648X]) negatively affect growth, feed conversion efficiency, health or mortality in Oreochromis niloticus. Microcystins do accumulate in muscle tissue (and particularly in the liver), which may exceed the upper limit of the tolerable daily intake of microcystins suggested by the WHO (0.04 μg/kg body weight/d).[http://www.sciencedirect.com/science/article/pii/S004484860600648X] Accumulation of microcystis increases lipoxidation (a biomarker of oxygen-mediated toxicity) in the liver.[http://www.sciencedirect.com/science/article/pii/S0166445X0600021X] Microcystin concentrations in the liver and intestine may gradually decrease as a result of the capacity in Tilapia to depurate and excrete microcystins into the bile and surrounding water, to avoid toxicity.[http://www.sciencedirect.com/science/article/pii/S0147651305000266] During the depuration period, muscle tissue may contain the highest concentration of microcystins, which may exceed the tolerable limit for humans (even in combined diets).[http://www.sciencedirect.com/science/article/pii/S0166445X04002279] Microcystis wesenbergii is non-toxic, less competitive, but more suited to low phosphorus environments than Microcystis aeruginosa.[http://www.researchgate.net/publication/236246459_Competition_between_toxic_Microcystis_aeruginosa_and_nontoxic_Microcystis_wesenbergii_with_Anabaena_PCC7120] Microcystis flos-aquae is more dominant in salinity above 3 ppt, whereas Microcystis_aeruginosa is dominant in salinity up to 3 ppt.[http://researchonline.nd.edu.au/era_sci__article/3/]&lt;br /&gt;
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==Periphyton==&lt;br /&gt;
Periphyton is a mixture of algae, cyanobacteria, heterotrophic microbes and detritus (mainly scraps of macrophytes and mud [http://www.cabdirect.org/abstracts/20023142580.html]) attached to submerged surfaces embedded in a slimy mucopolysaccharide matrix.[http://link.springer.com/article/10.1023/A:1022639805031#page-1] Oreochromis niloticus graze actively, with little selectivity, on the periphyton established on artificial substrates in cages. Both animal and plant material are removed to an equal extent, but less smaller particles are ingested.[http://www.sciencedirect.com/science/article/pii/S0044848699003658] Surface-grazing on periphyton is greater than on Microcystis aeruginosa. For tilapia, filter-feeding may be a relatively unimportant method of ingesting algae.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1993.tb00573.x/abstract] Tilapia mossambica feeds almost exclusively on periphytic detrital aggregate. (Assimilation efficiencies: organic matter 63%, protein 77%, carbohydrate 63%) [http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1981)110%3C239%3ADAAOPD%3E2.0.CO%3B2#.VCusqPl_s10] Feed conversion ratio of dry matter periphyton may be 2.81. Fish growth may be sustained on periphyton despite 55% (dry matter) ash content.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2003.00802.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] The fresh microbial mat was 81% digestible by Nile tilapia, comparing favorably with commercial catfish feed in digestibility by Nile tilapia. The dried form was significantly less digestible.[http://www.tandfonline.com/doi/abs/10.1577/1548-8640(1989)051%3C0083%3ADOACFF%3E2.3.CO%3B2#.VCu6hvl_s10] Tilapia are equipped with a &amp;#039;stomach bypass&amp;#039; to be able to bypass or regurgitate unwanted materials.[http://www.researchgate.net/publication/229614655_A_study_of_the_histology_of_the_digestive_tract_of_the_Nile_tilapia]&lt;br /&gt;
&lt;br /&gt;
==Algae meal==&lt;br /&gt;
Under most unnatural feeding conditions tilapia are unable to sufficiently ingest high volumes of algae. They may need constant grazing to fulfill their nutrient requirements.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1995.tb01868.x/abstract] Tilapia (Sarotherodon niloticus) grow better on fishmeal (fish meal may contain 74% protein and 8% lipids.[http://link.springer.com/article/10.1007/s10499-008-9207-5#page-1]) than on a 25% protein green algae meal (Cladophora glomerata). Weight gain decreased as the level of algal protein increased as replacement of fish meal. Protein digestibility was highest on a 5:1 ratio (fishmeal : green algae meal).[http://www.sciencedirect.com/science/article/pii/0044848683901485] Protein synthesis (with normal sulfur and carbon content) by green algae during the night may match protein synthesis during the day (in Dunaliella tertiolecta).[http://m.aslo.info/lo/toc/vol_29/issue_4/0731.pdf] Protein derived from algae does not promote adequate growth in Rainbow trout.[http://www.sciencedirect.com/science/article/pii/0044848678900972] Fish fed 5% ulva meal (Green algae; Ulva rigida) showed increased growth, feed conversion ratio and protein efficiency ratio.[http://link.springer.com/article/10.1007/s10499-008-9207-5#page-1] Ulva meal may replace soy bean meal to the extent of 20% without negatively affecting growth of male larval tilapia. Feed conversion ratio increased with increasing ulva meal content.[http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0426.2007.01017.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Green algae meal (Hydrodictyon reticulatum) may replace meal to the extent of 25% without negatively affecting growth of Oreochromis niloticus and Tilapia zillii fingerlings.[http://onlinelibrary.wiley.com/doi/10.1111/j.1095-8649.1985.tb04034.x/abstract] Spirulina maxima meal protein can replace up to 40% of the fish meal protein in Oreochromis mossambicus fry diets without negatively affecting growth.[http://www.researchgate.net/publication/230241662_Effect_of_the_use_of_the_microalga_Spirulina_maxima_as_fish_meal_replacement_in_diets_for_tilapia_Oreochromis_mossambicus_(Peters)_fry]&lt;br /&gt;
&lt;br /&gt;
==Off-flavor==&lt;br /&gt;
Cyanobacteria causing off-flavor (by geosmin and 2-methylisoborneol, MIB in fish flesh) are Oscillatoria tenuis, Anabaena circinalis and Pseudanabaena catenata. Tilapia flesh derived from cage culture provided a lower possibility of off-flavor contamination than those from earthen ponds.[http://www.cabdirect.org/abstracts/20133297816.html]&lt;br /&gt;
Geosmin (1,10-trans-dimethyl-trans-9-decaol) in fresh tilapia at concentrations ranging from 7.55 to 9.85 μg/kg of fish flesh is detected as &amp;#039;muddy flavor&amp;#039; by sensory evaluation. In brackish fish, only geosmin levels below 2.6 μg/kg remain undetected. The highest geosmin concentrations are contained in the intestines, followed by abdominal, skin and muscle tissues, susbsequently. It takes at least 16 days in static clean water to eliminate the muddy flavor from geosmin absorbed through the skin.[http://www.tandfonline.com/doi/abs/10.1300/J030v09n02_04#.VHywhYuG810]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4444</id>
		<title>Composting toilet</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4444"/>
		<updated>2014-12-28T17:33:38Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[Image:Urine_diversion.jpg|thumb|left| urine diversion ]]&lt;br /&gt;
[[Image:Composting_toilet.jpg|thumb|right| [http://en.wikipedia.org/wiki/Composting_toilet#mediaviewer/File:Clivus_Multrum_Composting_toilet_with_urine_diversion-dehydration.svg Clivus Multrum] adapted for solar ventilation and urine re-use&lt;br /&gt;
- 1. Humus compartment 2. Ventilation inflow 3. WC 4. Urinal 5. Urine collection 6. Manual paddles 7. Solar heating of ventilation outflow ]]&lt;br /&gt;
[[Image:Urine_diversion_2.jpg|thumb|left| urine diversion - squatting [http://en.wikipedia.org/wiki/Urine-diverting_dry_toilet#mediaviewer/File:Inside_of_the_UDDT_(5332645379).jpg]]]&lt;br /&gt;
&lt;br /&gt;
It is estimated that the excreta from 80 persons could fertilize a hectare. A single adult eats 250 kg of cereals per year, which may be grown on less than 250 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;, fertilised by ~50% of the urine of that person, mixed with the waste water used by that person.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf]&lt;br /&gt;
&lt;br /&gt;
Composting toilets aerobically decompose waste material, by aerobic microorganisms in a highly aerated, warm (32°-49°) and moist (humidity &amp;gt;80%) environment, with ample carbon (sawdust as the sole agent will lead to clogging[http://www.lismore.nsw.gov.au/infocouncil/Open/2013/OC_13082013_ATT_EXCLUDED_WEB.HTM]). During composting, mineralization, and humification occur simultaneously and are the main processes leading to degradation of the fresh organic matter. Organic matter humification in composting occurs when lignin and its degradation products are degraded by the microorganisms.[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] Optimally, the volume of the resulting compost may be about 10% of input.[http://en.wikipedia.org/wiki/Composting_toilet] &lt;br /&gt;
&lt;br /&gt;
Continuous composting toilets are single container toilets that receive excrement which decomposes as it moves slowly through the container and is removed as compost from the end-product chamber. The container is permanently fitted under the toilet seat, and never has to be fully emptied as the compost can be gradually removed when it reaches the end-product chamber. [http://www.yourhome.gov.au/water/waterless-toilets] To make the fresh material move down the slope, the slope needs to be slippery, eg bathroom tiles. Natural zeolite may be used as medium for the sorption of ammonia from wastewater and subsequently as nitrogen releaser in cultures of Spirulina platensis.[http://www.sciencedirect.com/science/article/pii/S0960852413019627]&lt;br /&gt;
&lt;br /&gt;
In a non-urine-diverting system, 90% of what goes into a composting toilet is water. Compost piles need to be damp to work well, but most composting toilets suffer from too much water. (ideally 45 to 70% moisture content [http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets])&lt;br /&gt;
Ammonia may evaporate as nitrogen in a gas form, which is vented out along with carbon dioxide and water vapor.[http://www.google.com/patents/US4096592]&lt;br /&gt;
Evaporation is the primary way a composting toilet gets rid of excess water. Warmth and air flow through the unit assist the evaporation process. Every composting toilet has a vertical vent pipe to carry off moisture. Air flows across the drying trays, around and through the pile, then up the vent to the outside of the building.&lt;br /&gt;
In a cold environment, the low-grade heat produced by composting is supposed to provide sufficient updraft to carry vapor up the vent.[http://buttecreekwatershed.org/BMP/test2/Book6last.htm] In tropical conditions, passive solar heating may create an air draft.&lt;br /&gt;
&lt;br /&gt;
If operation conditions for thermophilic composting are adequate (moisture content 50 to 60%, carbon to nitrogen ratio 30 to 35 and mixing with bulking material), the temperature will rise to between 50 and 65 °C (WHO 2006). Such temperatures will effectively inactivate pathogens (WHO 2006).[http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets] If moisture content is too high, anaerobic organisms will thrive, creating undesirable odors from anaerobic digestion.&lt;br /&gt;
&lt;br /&gt;
==Carbon / Nitrogen ratio==&lt;br /&gt;
Dry material, which contains carbon (such as dry leaves or grasses) increases composting properties. It regulates moisture and the carbon to nitrogen ratio (C/N) and enhances the composting process. The optimal carbon to nitrogen ratio for thermophilic composting is 20 to 35 (WHO 2006). The C/N ratio of humanure (135 to 270 g / person / day) is about 5 to 10 (5%-7% nitrogen) The C/N ratio of grass clippings may be 12-19 and they may contain 2.4% nitrogen. The C/N ratio of leaves may be 48 and they may contain 0.9% nitrogen.[http://www.weblife.org/humanure/chapter3_7.html] Thus 200 g humanure plus 225 g leaves may render a combined C/N ratio of 30.&lt;br /&gt;
The C/N ratio of Panicum texanum is 32.9:1.[http://www.researchgate.net/publication/24186263_Nematode_Population_Fluctuations_during_Decomposition_of_Specific_Organic_Amendments] Thus 200 g humanure plus 1379 to 1724 g. [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Panicum Panicum texanum] may render a combined C/N ratio of 30.&lt;br /&gt;
&lt;br /&gt;
Crude protein in the leaves of Giant cane ([http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Arundinaria_gigantea Arundinaria gigantea]) is relatively low in young leaves.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=8259377&amp;amp;fileId=S1742170510000566] The C/N ratio in Giant cane leaves may be 19.4 (2.4% nitrogen and 45.8% carbon).[http://www.ars.usda.gov/SP2UserFiles/ad_hoc/19320000PosterGallery/AFGC2004_Bamboo_Final.pdf] Bamboo shoots may contain 0.6% nitrogen. Overall bamboo litter (culm, branches, leaves) may contain 1.4% nitrogen.[http://www.sciencedirect.com/science/article/pii/S0378112704006693] The carbon concentration in decomposing bamboo culms may be 50%, irrespective of decomposition level. Mean nitrogen concentration may be 1%.(going up from ~0.3% as decomposition advances) [https://www.deepdyve.com/lp/springer-journals/characteristics-of-culm-structure-and-carbon-and-nitrogen-pP0nfb7X0z/7] Bamboo biochar and wood biochar have the same C/N ratio[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] The C/N ratio of softwood may be 640, of hardwood may be 560 and that of sawdust 500, which goes down as it rots (down to 200).[http://www.weblife.org/humanure/chapter3_7.html] That is because the level of carbon (and Na, K) goes down faster than the level of nitrogen (and P).[http://link.springer.com/article/10.1007/BF00346060#page-1] Nitrogen is usually retained until 50% of the carbon remains.[http://link.springer.com/article/10.1007/s10021-004-0026-x#page-1] For the supply of carbon, one may process wood into sawdust, or one may let the chopped wood decompose naturally (with a lower C/N ratio). &lt;br /&gt;
&lt;br /&gt;
For a resulting combined C/N ratio of 30:&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 21 g                       bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 11 g                       bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 63 g  partially decomposed bamboo wood    (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 24 g  partially decomposed bamboo culms   (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 252 g partially decomposed bamboo culms   (if C/N=50) &lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum   (C/N=32.9) + 200 g partially decomposed bamboo culms   (if C/N=50)&lt;br /&gt;
&lt;br /&gt;
Fruit wastes may have a C/N ratio of 20-50.[http://www.norganics.com/applications/cnratio.pdf] Mango waste (without stone) may have a C/N ratio of 50.[http://www.researchgate.net/profile/DrLakshmi_priya_Thyagarajan/publication/225038970_Bioconversion_of_Mango_Waste_Blended_with_Poultry_Waste_and_Cow_Dung_into_Useful_Manure_by_Aerobic_Composting/links/0fcfd4fbfa557c43e1000000]&lt;br /&gt;
&lt;br /&gt;
==Urine==&lt;br /&gt;
[[Image:Urine-nutrients.jpg|thumb|left| Urine composition - a [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_3.jpg|thumb|right| Urine composition - c [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_2.jpg|thumb|left| Urine composition - b [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
Excessive ammonia from urine inhibits the microbial processes in the chamber. With urine diversion, less bulking agent is needed and the C/N ratio is naturally enhanced.&lt;br /&gt;
The C/N ratio of urine (1-2 L / person / day) is about 0.8 and its nitrogen content is ~15-18%.[http://www.weblife.org/humanure/chapter3_7.html]&lt;br /&gt;
Urine can contain up to 90 percent of the nitrogen, up to 50 percent of the phosphorus, and up to 70 percent of the potassium present in human excreta.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf] It may contain 9.3 g/L urea, 1.9 g/L chloride, 1.2 g/L sodium, 0.8 g/L potassium and 0.2 g/L ammonia.[http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf] &lt;br /&gt;
In healthy individuals it is usually pathogen free, although undiluted it may contain inorganic salts and organic compounds at levels toxic to plants. Ureum decomposes into ammonia, which smells.&lt;br /&gt;
&lt;br /&gt;
When applying urine to pastures, 14 to 28% of the nitrogen may evaporate in 2 weeks. 50% of that will be lost within the first 24 hours.[http://www.publish.csiro.au/?paper=AR9820097] The level of nitrogen volatilized as ammonia increases with increasing temperature.[http://link.springer.com/article/10.1007/BF00335848] Urine can be used as a source of complementary nitrogen in high carbon compost. When diluted with water at a 1:8 ratio, it can be used as a fertilizer [http://www.liquidgoldbook.com/], comparable to commercial fertilizers.[http://modernfarmer.com/2014/01/human-pee-proven-fertilizer-future/] &lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Algae_for_Tilapia#Spirulina Spirulina platensis] may be grown on human urine [http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] &lt;br /&gt;
At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L.[http://link.springer.com/article/10.1007%2FBF02179776#page-1] Since human urine typically contains about 9.3 gram urea per L [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf], 1 L of urine may be added to 5.2 L of clean water (or 161 ml of urine per L clean water).&lt;br /&gt;
Spirulina platensis may use several nitrogen sources; most effectively sodium nitrate, ammonium nitrate and urea.[http://link.springer.com/article/10.1023/A:1011925022941#page-1] At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L [http://link.springer.com/article/10.1007%2FBF02179776#page-1]. Best cellular growth may occur at 500 mg/l of urea.[http://www.sciencedirect.com/science/article/pii/S0960852403002359] Growth may be optimally when the urea feeding rate is slowly increased [http://www.sciencedirect.com/science/article/pii/S0044848604005952] and at 29°C. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] Best results are obtained by continuous urea addition in exponentially increasing amount, at 30°C.[http://www.sciencedirect.com/science/article/pii/S0961953402000545] Intermittent addition of urea yields results similar to those obtained by the continuous feeding.[http://www.sciencedirect.com/science/article/pii/S0144860904000263] Spirulina platensis grows better on urea than on potassium nitrate.[http://link.springer.com/article/10.1385/ABAB:112:3:143#page-1]&lt;br /&gt;
Though Spirulina platensis may tolerate high levels of urea better [http://link.springer.com/article/10.1007/s10295-005-0025-8#page-1], it is ammonium resistant at high pH.[http://pcp.oxfordjournals.org/content/32/7/953.short] The uptake of ammonia in Spirulina platensis (supporting optimal growth) is pH dependent with an optimum at pH 9.3, proceeding at the same rates in the light and in the dark.[http://pcp.oxfordjournals.org/content/30/2/303.short] High productivity is achieved when ammonia is maintained at up to 50% of the total nitrogen.[http://www.sciencedirect.com/science/article/pii/S0960852410018213]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4439</id>
		<title>Composting toilet</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4439"/>
		<updated>2014-12-28T16:42:35Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[Image:Urine_diversion.jpg|thumb|left| urine diversion ]]&lt;br /&gt;
[[Image:Composting_toilet.jpg|thumb|right| [http://en.wikipedia.org/wiki/Composting_toilet#mediaviewer/File:Clivus_Multrum_Composting_toilet_with_urine_diversion-dehydration.svg Clivus Multrum] adapted for solar ventilation and urine re-use&lt;br /&gt;
- 1. Humus compartment 2. Ventilation inflow 3. WC 4. Urinal 5. Urine collection 6. Manual paddles 7. Solar heating of ventilation outflow ]]&lt;br /&gt;
[[Image:Urine_diversion_2.jpg|thumb|left| urine diversion - squatting [http://en.wikipedia.org/wiki/Urine-diverting_dry_toilet#mediaviewer/File:Inside_of_the_UDDT_(5332645379).jpg]]]&lt;br /&gt;
&lt;br /&gt;
It is estimated that the excreta from 80 persons could fertilize a hectare. A single adult eats 250 kg of cereals per year, which may be grown on less than 250 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;, fertilised by ~50% of the urine of that person, mixed with the waste water used by that person.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf]&lt;br /&gt;
&lt;br /&gt;
Composting toilets aerobically decompose waste material, by aerobic microorganisms in a highly aerated, warm (32°-49°) and moist (humidity &amp;gt;80%) environment, with ample carbon (sawdust as the sole agent will lead to clogging[http://www.lismore.nsw.gov.au/infocouncil/Open/2013/OC_13082013_ATT_EXCLUDED_WEB.HTM]). During composting, mineralization, and humification occur simultaneously and are the main processes leading to degradation of the fresh organic matter. Organic matter humification in composting occurs when lignin and its degradation products are degraded by the microorganisms.[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] Optimally, the volume of the resulting compost may be about 10% of input.[http://en.wikipedia.org/wiki/Composting_toilet] &lt;br /&gt;
&lt;br /&gt;
Continuous composting toilets are single container toilets that receive excrement which decomposes as it moves slowly through the container and is removed as compost from the end-product chamber. The container is permanently fitted under the toilet seat, and never has to be fully emptied as the compost can be gradually removed when it reaches the end-product chamber. [http://www.yourhome.gov.au/water/waterless-toilets] To make the fresh material move down the slope, the slope needs to be slippery, eg bathroom tiles. Natural zeolite may be used as medium for the sorption of ammonia from wastewater and subsequently as nitrogen releaser in cultures of Spirulina platensis.[http://www.sciencedirect.com/science/article/pii/S0960852413019627]&lt;br /&gt;
&lt;br /&gt;
In a non-urine-diverting system, 90% of what goes into a composting toilet is water. Compost piles need to be damp to work well, but most composting toilets suffer from too much water. (ideally 45 to 70% moisture content [http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets])&lt;br /&gt;
Ammonia may evaporate as nitrogen in a gas form, which is vented out along with carbon dioxide and water vapor.[http://www.google.com/patents/US4096592]&lt;br /&gt;
Evaporation is the primary way a composting toilet gets rid of excess water. Warmth and air flow through the unit assist the evaporation process. Every composting toilet has a vertical vent pipe to carry off moisture. Air flows across the drying trays, around and through the pile, then up the vent to the outside of the building.&lt;br /&gt;
In a cold environment, the low-grade heat produced by composting is supposed to provide sufficient updraft to carry vapor up the vent.[http://buttecreekwatershed.org/BMP/test2/Book6last.htm] In tropical conditions, passive solar heating may create an air draft.&lt;br /&gt;
&lt;br /&gt;
If operation conditions for thermophilic composting are adequate (moisture content 50 to 60%, carbon to nitrogen ratio 30 to 35 and mixing with bulking material), the temperature will rise to between 50 and 65 °C (WHO 2006). Such temperatures will effectively inactivate pathogens (WHO 2006).[http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets] If moisture content is too high, anaerobic organisms will thrive, creating undesirable odors from anaerobic digestion.&lt;br /&gt;
&lt;br /&gt;
==Carbon / Nitrogen ratio==&lt;br /&gt;
Dry material, which contains carbon (such as dry leaves or grasses) increases composting properties. It regulates moisture and the carbon to nitrogen ratio (C/N) and enhances the composting process. The optimal carbon to nitrogen ratio for thermophilic composting is 20 to 35 (WHO 2006). The C/N ratio of humanure (135 to 270 g / person / day) is about 5 to 10 (5%-7% nitrogen) The C/N ratio of grass clippings may be 12-19 and they may contain 2.4% nitrogen. The C/N ratio of leaves may be 48 and they may contain 0.9% nitrogen.[http://www.weblife.org/humanure/chapter3_7.html] Thus 200 g humanure plus 225 g leaves may render a combined C/N ratio of 30.&lt;br /&gt;
The C/N ratio of Panicum texanum is 32.9:1.[http://www.researchgate.net/publication/24186263_Nematode_Population_Fluctuations_during_Decomposition_of_Specific_Organic_Amendments] Thus 200 g humanure plus 1379 to 1724 g. [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Panicum Panicum texanum] may render a combined C/N ratio of 30.&lt;br /&gt;
&lt;br /&gt;
Crude protein in the leaves of Giant cane ([http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Arundinaria_gigantea Arundinaria gigantea]) is relatively low in young leaves.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=8259377&amp;amp;fileId=S1742170510000566] The C/N ratio in Giant cane leaves may be 19.4 (2.4% nitrogen and 45.8% carbon).[http://www.ars.usda.gov/SP2UserFiles/ad_hoc/19320000PosterGallery/AFGC2004_Bamboo_Final.pdf] Bamboo shoots may contain 0.6% nitrogen. Overall bamboo litter (culm, branches, leaves) may contain 1.4% nitrogen.[http://www.sciencedirect.com/science/article/pii/S0378112704006693] The carbon concentration in decomposing bamboo culms may be 50%, irrespective of decomposition level. Mean nitrogen concentration may be 1%.(going up from ~0.3% as decomposition advances) [https://www.deepdyve.com/lp/springer-journals/characteristics-of-culm-structure-and-carbon-and-nitrogen-pP0nfb7X0z/7] Bamboo biochar and wood biochar have the same C/N ratio[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] The C/N ratio of softwood may be 640, of hardwood may be 560 and that of sawdust 500, which goes down as it rots (down to 200).[http://www.weblife.org/humanure/chapter3_7.html] That is because the level of carbon (and Na, K) goes down faster than the level of nitrogen (and P).[http://link.springer.com/article/10.1007/BF00346060#page-1] Nitrogen is usually retained until 50% of the carbon remains.[http://link.springer.com/article/10.1007/s10021-004-0026-x#page-1] For the supply of carbon, one may process wood into sawdust, or one may let the chopped wood decompose naturally (with a lower C/N ratio). &lt;br /&gt;
&lt;br /&gt;
For a resulting combined C/N ratio of 30:&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 21 g bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum (C/N=32.9) + 11 g bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 63 g partially decomposed bamboo wood (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum (C/N=32.9) + 24 g partially decomposed bamboo culms (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 252 g partially decomposed bamboo culms (if C/N=50) &lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum (C/N=32.9) + 200 g partially decomposed bamboo culms (if C/N=50)&lt;br /&gt;
&lt;br /&gt;
Fruit wastes may have a C/N ratio of 20-50.[http://www.norganics.com/applications/cnratio.pdf] Mango waste {without stone) may have a C/N ratio of 50.[http://www.researchgate.net/profile/DrLakshmi_priya_Thyagarajan/publication/225038970_Bioconversion_of_Mango_Waste_Blended_with_Poultry_Waste_and_Cow_Dung_into_Useful_Manure_by_Aerobic_Composting/links/0fcfd4fbfa557c43e1000000]&lt;br /&gt;
&lt;br /&gt;
==Urine==&lt;br /&gt;
[[Image:Urine-nutrients.jpg|thumb|left| Urine composition - a [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_3.jpg|thumb|right| Urine composition - c [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_2.jpg|thumb|left| Urine composition - b [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
Excessive ammonia from urine inhibits the microbial processes in the chamber. With urine diversion, less bulking agent is needed and the C/N ratio is naturally enhanced.&lt;br /&gt;
The C/N ratio of urine (1-2 L / person / day) is about 0.8 and its nitrogen content is ~15-18%.[http://www.weblife.org/humanure/chapter3_7.html]&lt;br /&gt;
Urine can contain up to 90 percent of the nitrogen, up to 50 percent of the phosphorus, and up to 70 percent of the potassium present in human excreta.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf] It may contain 9.3 g/L urea, 1.9 g/L chloride, 1.2 g/L sodium, 0.8 g/L potassium and 0.2 g/L ammonia.[http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf] &lt;br /&gt;
In healthy individuals it is usually pathogen free, although undiluted it may contain inorganic salts and organic compounds at levels toxic to plants. Ureum decomposes into ammonia, which smells.&lt;br /&gt;
&lt;br /&gt;
When applying urine to pastures, 14 to 28% of the nitrogen may evaporate in 2 weeks. 50% of that will be lost within the first 24 hours.[http://www.publish.csiro.au/?paper=AR9820097] The level of nitrogen volatilized as ammonia increases with increasing temperature.[http://link.springer.com/article/10.1007/BF00335848] Urine can be used as a source of complementary nitrogen in high carbon compost. When diluted with water at a 1:8 ratio, it can be used as a fertilizer [http://www.liquidgoldbook.com/], comparable to commercial fertilizers.[http://modernfarmer.com/2014/01/human-pee-proven-fertilizer-future/] &lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Algae_for_Tilapia#Spirulina Spirulina platensis] may be grown on human urine [http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] &lt;br /&gt;
At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L.[http://link.springer.com/article/10.1007%2FBF02179776#page-1] Since human urine typically contains about 9.3 gram urea per L [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf], 1 L of urine may be added to 5.2 L of clean water (or 161 ml of urine per L clean water).&lt;br /&gt;
Spirulina platensis may use several nitrogen sources; most effectively sodium nitrate, ammonium nitrate and urea.[http://link.springer.com/article/10.1023/A:1011925022941#page-1] At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L [http://link.springer.com/article/10.1007%2FBF02179776#page-1]. Best cellular growth may occur at 500 mg/l of urea.[http://www.sciencedirect.com/science/article/pii/S0960852403002359] Growth may be optimally when the urea feeding rate is slowly increased [http://www.sciencedirect.com/science/article/pii/S0044848604005952] and at 29°C. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] Best results are obtained by continuous urea addition in exponentially increasing amount, at 30°C.[http://www.sciencedirect.com/science/article/pii/S0961953402000545] Intermittent addition of urea yields results similar to those obtained by the continuous feeding.[http://www.sciencedirect.com/science/article/pii/S0144860904000263] Spirulina platensis grows better on urea than on potassium nitrate.[http://link.springer.com/article/10.1385/ABAB:112:3:143#page-1]&lt;br /&gt;
Though Spirulina platensis may tolerate high levels of urea better [http://link.springer.com/article/10.1007/s10295-005-0025-8#page-1], it is ammonium resistant at high pH.[http://pcp.oxfordjournals.org/content/32/7/953.short] The uptake of ammonia in Spirulina platensis (supporting optimal growth) is pH dependent with an optimum at pH 9.3, proceeding at the same rates in the light and in the dark.[http://pcp.oxfordjournals.org/content/30/2/303.short] High productivity is achieved when ammonia is maintained at up to 50% of the total nitrogen.[http://www.sciencedirect.com/science/article/pii/S0960852410018213]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Mango&amp;diff=4429</id>
		<title>Mango</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Mango&amp;diff=4429"/>
		<updated>2014-12-27T14:14:45Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling, Spacing&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Comparable to oranges and clementines, mangoes are among the most all-round fruits, regarding nutrients. Mangoes are cultivated in (sub)tropical regions all over the world.&lt;br /&gt;
&lt;br /&gt;
=Low-fibre, sweet mango species=&lt;br /&gt;
If you use a juice extractor like the [http://www.thekitchn.com/product-review-hurom-slow-juic-131492 Hurom] / [http://versapers.fr/ Versapers] (masticating slow juicer), the stringy fibres from species like Tommy Atkins or Haden will clog your juicer (more so than pineapples). The chamber will fill up with long fibres that simply never get discarded, so that you need to empty and clean it after every few mangoes. With mangoes like Kent (#11 on the list), you may endlessly (&amp;gt;100 mangoes) continue juicing without having to empty or clean the chamber in between, because the short fibres are immediately discarded as pulp (as with apples, pears, clementines).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Anwar Ratol==&lt;br /&gt;
[[Image:Anwar_Ratol.jpg|thumb|right| Anwar Ratol [http://freshfruitpakistan.blogspot.nl/2013/04/anwar-ratol-top-variety-of-mango.html © H. Khalid]]]&lt;br /&gt;
&lt;br /&gt;
* #1 (aka Anwer Rathol, Anwar Retaul or Anwar Rataul)&lt;br /&gt;
* Small size (av. 180 g), medium thick skin&lt;br /&gt;
* Matures from green to pale bright yellow &lt;br /&gt;
* Very sweet (TSS 26-28%)&lt;br /&gt;
* Fibreless flesh, medium juicy&lt;br /&gt;
* Originally from Rataul, in Bagpat district, in the province of Uttar Pradesh, India. &lt;br /&gt;
* Harvested from June to July (India and Punjab in Pakistan)&lt;br /&gt;
* Keeps well in storage&lt;br /&gt;
&lt;br /&gt;
==Alphonso==&lt;br /&gt;
[[Image:Alphonso.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #2 (aka Alphanso, Bombay)&lt;br /&gt;
* Small, roundish / oblong (av. 200 g.)&lt;br /&gt;
* Smooth, (from green to) pale greenish-yellow to deep orange peel (deepening with ripening)&lt;br /&gt;
* Smooth, firm flesh. Intense reddish-orange colour&lt;br /&gt;
* Sweet, somewhat sour (17% sugar; sugar/acid ratio: 39; TSS 21-23%) and flavoured&lt;br /&gt;
* Virtually without fibre (1%), medium juicy&lt;br /&gt;
* Originally from Western India&lt;br /&gt;
* Main production in Maharashtra and Gujarat&lt;br /&gt;
* Highest quality in Sindhudurg, Ratnagiri (Natwarlal plantation), Raigad and Thane districts&lt;br /&gt;
* Harvested from early April to early July (India) and July (some districts in Sindh, Pakistan)&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
* Tolerates high humidity&lt;br /&gt;
* Good keeping quality (up to 4 weeks at 11° C)&lt;br /&gt;
&lt;br /&gt;
==Dashehari==&lt;br /&gt;
[[Image:Dashehari.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #3 (aka Dasheri, Doshehri, Aman Dusehri or Dussehri)&lt;br /&gt;
* Small, oblong (av. 175 g)&lt;br /&gt;
* Matures from light green to light yellow, thin skin&lt;br /&gt;
* Sweet (TSS 21-22%), lemon yellow flesh&lt;br /&gt;
* Fibreless, medium juicy&lt;br /&gt;
* Very small stone&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Dasheri, Uttar Pradesh, India.&lt;br /&gt;
* Harvested from early June to July, mainly Malihabad, Uttar Pradesh (and Punjab)&lt;br /&gt;
&lt;br /&gt;
==Bagan Pali==&lt;br /&gt;
[[Image:Bagan_Pali.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #4 (aka Begun Palli, Began Phali, Banganapalli or Baganpali)&lt;br /&gt;
* Large (av. 550 g.), oval&lt;br /&gt;
* Sweet (TSS 18-20%)&lt;br /&gt;
* Fibreless, little juicy, yellow flesh &lt;br /&gt;
* Matures from dark green to yellow light green or yellow-orange&lt;br /&gt;
* Thin, smooth, shiny skin&lt;br /&gt;
* Originally from Sindh, Pakistan and/or Banganapalle in Andhra Pradesh, India.&lt;br /&gt;
* Harvested from July to August (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Sindhri==&lt;br /&gt;
[[Image:Sindhri.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #5 (aka Sindhry)&lt;br /&gt;
* Medium to large (av. 300 g., av. 400 g in Pakistan)&lt;br /&gt;
* Elongated with pointy curve&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 36; TSS 15-17%), flavoured&lt;br /&gt;
* Little fibre (3% - 5%), little juicy&lt;br /&gt;
* From green to deep matt lemon yellow thin peel (somewhat wrinkly) when ripe&lt;br /&gt;
* Deep yellow-orange flesh; soft and melting&lt;br /&gt;
* Originally from Mir Pur Khaas in Sindh&lt;br /&gt;
* Harvested from late May to July (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Chaunsa==&lt;br /&gt;
[[Image:Chaunsa.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #6 (aka Sammar Bahisht Chausa)&lt;br /&gt;
* Large (av. 350 g.), oblong&lt;br /&gt;
* Sweet (18% sugar; sugar/acid ratio: 58; TSS 18-20%)&lt;br /&gt;
* Soft, succulent flesh &lt;br /&gt;
* Medium fibre, large stone, medium juicy (delicious for eating, too stringy for the slow juicer)&lt;br /&gt;
* Thin, pale, matt yellow-greenish peel (wrinkly and slightly deeper when ripe)&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Pakistan&lt;br /&gt;
* Harvested mainly in the Rahim Yar Khan and Multan (Sahiwal) districts of Punjab in Pakistan&lt;br /&gt;
* Harvested from June to early September (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Maya==&lt;br /&gt;
&lt;br /&gt;
* #7&lt;br /&gt;
* Medium, round (av. 300 g.)&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 42; TSS 16-17%) and flavoured&lt;br /&gt;
* Red blush peel (with yellow tinge when ripe)&lt;br /&gt;
* Smooth, deep/pale yellow flesh (soft when ripe)&lt;br /&gt;
* Medium fibre, very juicy&lt;br /&gt;
* Harvested from mid-June to early July (Gambia)&lt;br /&gt;
&lt;br /&gt;
==Gopalbogh==&lt;br /&gt;
&lt;br /&gt;
* #8 (aka Gopal Bhogue)&lt;br /&gt;
* Small size (average 190 g.)&lt;br /&gt;
* Yellow-green peel, with small seeds&lt;br /&gt;
* Virtually without fibre&lt;br /&gt;
* Very sweet (21% sugar)&lt;br /&gt;
* Good quality mainly grown in Rajshahi region, especially in Chapainawabganj district (Bangladesh).&lt;br /&gt;
* Harvest from mid May to mid June (Bangladesh)&lt;br /&gt;
&lt;br /&gt;
==Sweet Elena==&lt;br /&gt;
[[Image:Sweet_Elena.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #9&lt;br /&gt;
* Large sized, ovoid (av. 350 g.)&lt;br /&gt;
* Thin, orange peel&lt;br /&gt;
* Yellow to orange flesh&lt;br /&gt;
* Little fibre&lt;br /&gt;
* Sweet (19% sugar)&lt;br /&gt;
* Originally (as a carabao variety) from Sta. Cruz, Zambales (Philippines)&lt;br /&gt;
* Harvest from March to April (Locloc, Palauig in northern Zambales, Philippines)&lt;br /&gt;
&lt;br /&gt;
==Ataulfo==&lt;br /&gt;
[[Image:Ataulfo.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #10 (aka Adaulfo, Adolfo, Champagne, Manila or honey mango, related to Alphonso)&lt;br /&gt;
* Small, oblong shape (av. 225 g.)&lt;br /&gt;
* With ripening, peel turns from pale green to gold-yellow (yellow to orange and wrinkles when fully ripe)&lt;br /&gt;
* Deep yellow flesh (gold when ripe), little fibre and thin pit&lt;br /&gt;
* Smooth, sweet (15% sugar), and buttery / creamy flesh&lt;br /&gt;
* Spicy flavour &lt;br /&gt;
* Best temperature is 28°C, tolerates 1100 to 3000 mm annual rainfall &lt;br /&gt;
* Originally from Chiapas, Mexico&lt;br /&gt;
* Harvest from mid-October to late December (Ecuador); February to August (Ghana)&lt;br /&gt;
* May be stored in the fridge for 2 weeks&lt;br /&gt;
&lt;br /&gt;
==Kent==&lt;br /&gt;
[[Image:Kent.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #11&lt;br /&gt;
* Large, rounded oval (500 to 800 g.)&lt;br /&gt;
* Smooth, waxy thick peel (green with some yellow and red; yellow increases with ripening)&lt;br /&gt;
* Soft, melting, juicy flesh (from deep yellow to deep orange when fully ripe)&lt;br /&gt;
* Limited fibre (dry weight: 0.4 - 2%), small seed&lt;br /&gt;
* Sweet (13% sugar; 14ºBrix [http://www.m.elewa.org/JAPS/2013/17.3/5.pdf])&lt;br /&gt;
* Originally from Mexico / Florida&lt;br /&gt;
* Harvest from January to March and May to August (Ghana)&lt;br /&gt;
* Large tree (up to 20 m.)&lt;br /&gt;
* No storage keeping below 13°C&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
=Mango Cultivation=&lt;br /&gt;
==Conditions==&lt;br /&gt;
The best soil for mango trees has a pH between 5.5 and 7.5 (high pH may cause leaf tip burn). The temperature range for growing mangoes is 15 to 40°C. Mango does best within a temperature range of 24 to 27°C. The Indian race is in general intolerant of humidity (susceptible to anthracnose), the Philippine race tolerates excess moisture. The life span of each tree is approximately 100 years. Economic bearing life is 30 to 50 years.&lt;br /&gt;
Mango trees need deep soil (up to 2 m) for their root systems. Grafted saplings yield a more superior quality of fruit. Fruits are borne by mango trees from 4 to 5 years after planting. Full bearing starts at 6 to 7 years.&lt;br /&gt;
Grafted mango plants start bearing blossoms one to two years after planting, provided that there is no high humidity or intense rain during the flowering period. Mango is successfully cultivated in areas with annual precipitation of 300-2500 mm. For successful cultivation, rainfall distribution is essential, as a dry spell of 3 to 4 months is required for flowering, fruit set and fruit development. Without flowering there will be no fruit set.&lt;br /&gt;
Soils with high clay content or frequent water logging are not suitable. &lt;br /&gt;
Ideal soil for mango is deep red loam with loose gravel.[https://books.google.nl/books?id=QEVHhCKBT-IC&amp;amp;dq=annual+precipitation+mango+cultivation&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
A dry spell of 4 months between flowering and fruit harvest is ideal.[https://books.google.nl/books?id=u3lwAgAAQBAJ&amp;amp;dq=annual+precipitation+mango+cultivation&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s]&lt;br /&gt;
&lt;br /&gt;
==Rootstock Propagation==&lt;br /&gt;
[[Image:Mango_grafting.jpg|thumb|left| [http://www.muftisays.com/blog/ummi+taalib/2718_20-03-2012/shaikhmurid-relationship.html © ummi taalib]]] &lt;br /&gt;
[[Image:Grafted_seedling.jpg|thumb|centre| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
[[Image:Grafted_seedlings.jpg|thumb|right| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
Trees grown from mango seeds (and naturally, sexually propagated) will be very different from the tree those mangoes came from (such &amp;#039;wild mangoes&amp;#039; usually taste bad). That is why rootstocks (raised from seeds) are asexually propagated. Matured seedlings (about 60 cm tall) are grafted by inarching; a bud from a tree producing good quality mangoes is artificially attached to the rootstock. This will create a new tree with two parts: the lower (wild) part and the upper part, which is an exact copy of the tree you took this bud from. Any bud growing from the lower part needs to be pruned off immediately. The upper part needs to grow and produce branches, and eventually fruits. During the first month after planting, the young trees need to be irrigated every 3 days. During the next 2 months, they need to be irrigated once a week. After that they should not be watered for a couple of months. Each year they need a dry season of a few months. Young plants need 10 to 30 kg organic manure per plant. Don&amp;#039;t use a chemical fertilizer on young plants. Once the tree is 2 years old, you may start using a controlled release fertilizer (with higher nitrogen), like 12-5-9 or 12-8-34 (= ratios N:P:K); 1 tbsp. in 1 L warm water / meter tree height. Or just manure.  &lt;br /&gt;
&lt;br /&gt;
Rootstock branches should always be pruned off. To prevent viral infections, only prune using a very sharp, sterilized knife (sterilize on the spot with a propane torch).&lt;br /&gt;
&lt;br /&gt;
==Production technology== &lt;br /&gt;
Space mango trees about 12 meters apart. Adult mango trees need about 500-750 mm of water per year. Irrigation at one week intervals results in the greatest yield, compared to 2 week intervals or no irrigation at all.[http://www.scielo.br/scielo.php?pid=S1677-04202007000400004&amp;amp;script=sci_arttext] Mango trees need dry and wet spells alternately (also kills various pathogens). Don&amp;#039;t fertilize mature trees during fruit bearing, and not too much irrigation. If during the blooming season the soil contains much nutrients (fertilizer) and water, the tree will grow, but not flower and bear fruits. &lt;br /&gt;
You may either:&lt;br /&gt;
* A: Apply 80 to 100 kg manure per year (eg humanure from [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets]), after all fruits are picked. Or:&lt;br /&gt;
* B: Apply 3-4 kg SSP, 2-3 kg Potassium Sulphate and 2-3 kg Urea before flowering. Plus 2-3 kg Urea in 2 doses after fruit setting. Or:&lt;br /&gt;
* C: Use a controlled release fertilizer once a year, such as 4-4-8 (= ratios N:P:K), after all fruits are picked (1 tbsp. in 1 L warm water / meter tree height).&lt;br /&gt;
 &lt;br /&gt;
Fruit size is most strongly affected by the initial fruit dry mass after cell division.[http://treephys.oxfordjournals.org/content/25/5/583.full.pdf] Early water stress influences final fruit size through an effect on the cell number. The critical period for fruit development is during the first 6 to 8 weeks when cell division and cell wall synthesis occur. Maintaining soil moisture at &amp;gt;60% of capacity improves fruit quality and yield. As fruit approaches maturity, water needs to be withheld, to increase sugar content.[https://books.google.nl/books?id=u3lwAgAAQBAJ&amp;amp;dq=annual+precipitation+mango+cultivation&amp;amp;hl=nl&amp;amp;source=gbs_navlinks_s] A water shortage close to harvest (eg 1.5 weeks before harvest) has no effect on fruit size.[http://www.scielo.br/scielo.php?pid=S1677-04202007000400004&amp;amp;script=sci_arttext] Increased levels of (water-soluble) fructose induced by late water-stress may be due to its contribution to osmotic adjustment.[http://www.sciencedirect.com/science/article/pii/S0168945297001052]&lt;br /&gt;
&lt;br /&gt;
One should aim at horizontal canopies instead of vertical canopies. Fruit growth is mainly affected by the availability of carbohydrates. Fruit size and dry matter contents are lower in fruits positioned lower in the canopy. Leaf nitrogen on an area basis reflects photosynthetic capacity, which is linearly related to the fraction of intercepted light. Shaded leaves have the lowest assimilation of carbon. Lower carbon assimilation results in lower dry mass (incl.sugars) contents.[http://www.scielo.br/scielo.php?pid=S1677-04202007000400004&amp;amp;script=sci_arttext] &lt;br /&gt;
&lt;br /&gt;
An increase in leaf-to-fruit ratio has a positive effect on sweetness [http://www.jhortscib.org/Vol73/73_3/12.htm] and a negative one on sourness [http://www.publish.csiro.au/paper/AR05159.htm]. Light exposure and leaf to fruit ratio determine the assimilation of sugars into the fruit. Excess nitrogen increases this ratio too much, increasing susceptibility to disease. Fruit growth rate, sucrose concentration, structural dry matter and total dry matter (structural-to-total dry matter ratio is not affected[http://onlinelibrary.wiley.com/doi/10.1002/jsfa.1968/abstract]) is greater when increasing the leaf:fruit ratio (ranging from 10 to 150 leaves per fruit). Water and dry matter accumulation are strongly positively correlated, and most marked at ratios &amp;gt; 100.[http://www.actahort.org/books/645/645_54.htm] Low assimilated supply increases the ratios of calcium (ratio &amp;lt; 60[http://www.actahort.org/books/464/464_3.htm]), glucose, fructose, malic and citric acid to structural dry weight, but not glucose concentration. Irrigation strongly affects fructose concentration and starch breakdown.[http://onlinelibrary.wiley.com/doi/10.1002/jsfa.1968/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Starch breakdown mainly leads to an increase in sucrose concentration rather than an increase in glucose concentration.[http://www.scielo.br/scielo.php?pid=S1677-04202007000400004&amp;amp;script=sci_arttext]  &lt;br /&gt;
&lt;br /&gt;
Peel-light exposure also converts green to red colouring of the peel by stimulating anthocyanin production.[http://www.sciencedirect.com/science/article/pii/S0925521497000392]&lt;br /&gt;
&lt;br /&gt;
 &lt;br /&gt;
After fruit harvest, prune off diseased, dried, broken branches and those touching the ground. Every 3 to 4 years about 15 to 20% of old wood should be removed.&lt;br /&gt;
Picking should be done when the fruit is fully developed and mature. Natural drop of the fruit is the main indication that the fruit is ready for picking. Expected yields vary from 40 to 100 kg per tree.&lt;br /&gt;
&lt;br /&gt;
One cannot rear any animals (except for carnivores) on land with mango trees, as mango leaves are deadly poisonous.&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
.&lt;br /&gt;
&lt;br /&gt;
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&lt;br /&gt;
=Bugs=&lt;br /&gt;
&lt;br /&gt;
==Aphids==&lt;br /&gt;
[[Image:Propylea_quatuordecimpunctata.jpg|thumb|right| Predatory ladybird [http://www.colpolon.biol.uni.wroc.pl/propylea%20quatuordecimpunctata.htm]]]&lt;br /&gt;
[[Image:Aphids_on_mango.png|thumb|left| Aphids [http://www.infonet-biovision.org/default/ct/124/crops#_1804_1202 © Varela]]]&lt;br /&gt;
[[Image:Mallada_sp_2.jpg|thumb|right| Mallada sp. © Texas University]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Aphids (plant lice; Toxoptera odinae) are small (1-4 mm), living in clusters. They pierce plant tissue and suck the sap of the leaves, which may cause the leaves to bend, twist or roll up. Aphids also transmit plant viruses. Some species produce winged offspring, &amp;quot;alates&amp;quot;, that may readily disperse. Dry (and warm) weather stimulates increases in aphid numbers. Some species of ants (eg Dolichoderus cuspidatus and Formica aerate) protect and &amp;#039;farm&amp;#039; aphids, feeding on the honeydew released by the aphids&amp;#039; alimentary (gut) canals. Insects secreting honeydew may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould] &lt;br /&gt;
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[u]Control[/u]: Plant flowering plants at the boarders to attract beneficial insects. Plant &amp;#039;trap crops&amp;#039; (dill, nasturtiums, timothy grass) to monitor aphid numbers. Check the trap crops every 3 days. Infested trap crops need to be burned. Aphid&amp;#039;s natural enemies include predatory ladybirds (eg Propylea quatuordecimpunctata), hooverfly larvae, parasitic wasps, aphid midge larvae, crab spiders (Thomisidae) and lacewings (Neuroptera). Exposing aphid populations to natural predators may be successful particularly when its hot, because once bitten they release an alarm pheromone and the others all jump off the plant. Jumping aphids may experience a heat shock (&amp;gt; 35°C) since the ground may be much warmer than the shady tree. This heat shock usually sterilizes the aphids (killing the bacteria on aphids that provide nutrients essential for aphid reproduction). [http://www.sciencedaily.com/releases/2007/04/070419172046.htm]&lt;br /&gt;
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[u]Crab spiders (Thomisidae)[/u]; Crab spiders are ambush hunters (they don&amp;#039;t build webs) that not just eat aphids, but may also hunt ants that farm aphids. Some of these crab spiders, Xysticus sp, may however not just eat ants, but also predatory beetles (that eat aphids).&lt;br /&gt;
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[u]Green lacewings (Chrysopidae)[/u]; One may buy millions of lacewings (as captive-bred eggs) for biological pest control, as reared for that purpose in many countries. A female lacewing may plant over 100 eggs on plants infested with aphids. These eggs hang on a thin 1 cm long stalk, most often under a leaf. The larvae of most species of lacewings are specialised predators that eat aphids (and coccids such as mango scales, and caterpillars). These larvae sway their heads from left to right, and back, until they strike something they can eat. They have mouthparts that can pierce and suck the aphids. Many (adult) lacewing species also feed on nectar and pollen, but particularly Chrysopidae (eg Mallada signata, Mallada boninensis) are mainly predatory (one adult may eat 15 aphids per day). One may attract Chrysopidae by planting crops that attract them (mainly Asteraceae and Apiaceae), such as calliopsis (Coreopsis), cosmos (Cosmos), sunflowers (Helianthus), dandelion (Taraxacum), dill (Anethum) and angelica (Angelica). &lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option and immediate action is required: Use Folido 50% EC at the rate of 0.45 L per 450 L water / acre. (also kills natural aphid enemies)&lt;br /&gt;
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==Fruit Flies==&lt;br /&gt;
[[Image:Fruit_fly_maggots.jpg|thumb|centre| Maggots [http://www.infonet-biovision.org/default/ct/124/crops © A. M. Varela]]]&lt;br /&gt;
[[Image:Bactrocera_invadens.jpg|thumb|left| B. invadens [https://www.flickr.com/photos/iaea_imagebank/6776806998/ © IAEA]]]&lt;br /&gt;
[[Image:Fopius_arisanus.jpg|thumb|right| F. arisanus [http://www.tephritid.com/digital.php?act=page&amp;amp;pid=28&amp;amp;id=25 © tephritid.com]]]&lt;br /&gt;
[[Image:Ceratitis_capitata.jpg|thumb|left| C. capitata [http://josedotinzulza.edublogs.org/2013/04/21/how-much-damage-can-make-a-fly/ © José Inzulza]]]&lt;br /&gt;
[[Image:Diachasmimorpha_longicaudata.jpg|thumb|right| D. longicaudata [http://www.oocities.org/brisbane_parawasps/FruitFlyParasitoid.htm]]]&lt;br /&gt;
[[Image:Ceratitis_fasciventris.jpg|thumb|left| C. fasciventris [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Oecophylla_longinoda.jpg|thumb|right| O. longinoda [http://www.alexanderwild.com/Ants/Taxonomic-List-of-Ant-Genera/Oecophylla/i-tMfJXKG © A.Wild]]]&lt;br /&gt;
[[Image:Ceratitis_cosyra.jpg|thumb|left| C. cosyra [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Jackson_trap.jpg|thumb|right| Jackson trap]]&lt;br /&gt;
[[Image:Ceratitis_rosa.jpg|thumb|left| C. rosa [http://wzciq.wenzhou.gov.cn/art/2011/1/12/art_6811_63595.html © wzciq]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis eating fly [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
In 2013, the export of mangoes from Ghana and 27 other African countries was banned from major international markets mainly due to fruit fly infestation. The European Union imposed a ban from May 1 2014, on import of mangoes from India, also due to fruit fly infestation. &lt;br /&gt;
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The fruit flies (aka peacock flies; 4-7 mm long) that may form a very severe mango cultivation pest are not the Drosophilae (2-4 mm long), but the colourful Tephritidae family (&amp;gt; 5000 species). These attack mango fruits throughout the season and lay their eggs right under the (affected) skin of mature green or ripe fruits. They have three generations and multiply very rapidly. Within one or two days the eggs hatch into white maggots. The maggots feed on the fruit, which starts to rot. After 4 to 17 days, the maggots make holes in the skin and leave the fruit to pupate. &lt;br /&gt;
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The main fruit fly pests of mango in Africa are Ceratitis capitata (Medfly), C. fasciventris, C. rosa and C. cosyra (the most destructive). C. cosyra is however rapidly displaced by Bactrocera invadens (endemic to Sri Lanka)[http://www.ncbi.nlm.nih.gov/pubmed/19610411][http://www.ncbi.nlm.nih.gov/pubmed/22251685], being predominantly a low-land pest.[http://www.ncbi.nlm.nih.gov/pubmed/16923206] B. invadens populations quickly increase with the onset of the raining season[http://www.ncbi.nlm.nih.gov/pubmed/19449630] and also lay eggs in young fruits. Fruit fly populations peak in the late dry season.[http://www.ncbi.nlm.nih.gov/pubmed/17598527]&lt;br /&gt;
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[u]Control[/u]: Collect all the fallen and affected fruits and bury them deep (&amp;gt;50 cm) into the soil. Or you can use them to breed maggots in a sealed room for fish feed. Natural enemies of tephritids include Diapriidae (tiny wasps; &amp;lt;8mm), Braconidae (also parasitoid wasps) and Oecophylla longinoda (Latreille; a weaver ant). &lt;br /&gt;
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C. cosyra, but particularly C. fasciventris and Medfly may be effectively controlled by using the host-marking pheromone.[http://www.ncbi.nlm.nih.gov/pubmed/23356072] B. invadens [http://www.ncbi.nlm.nih.gov/pubmed/24293246], the Mediterranean fruit fly (C. capitata) and the Mexican fruit fly (Anastrepha ludens) may be effectively controlled by the &amp;#039;sterile insect technique&amp;#039; (releasing overwhelming numbers of sterile insects). The effects of sterile flies are greater than those for parasitoid wasps, but they are complementary.[http://www.ncbi.nlm.nih.gov/pubmed/15568340] &lt;br /&gt;
Medfly eggs and instars (right under the peel) may be killed by immersing Ataulfo mangoes for 95 min in warm water at 47°C, also positively modifying pH (producing more palatable fruits), but can also produce a loss of firmness and weight (5%).[http://www.ncbi.nlm.nih.gov/pubmed/23356057] B. invadens is no more heat tolerant than Medfly.[http://www.ncbi.nlm.nih.gov/pubmed/21404834] &lt;br /&gt;
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[u]Bait traps[/u]; &lt;br /&gt;
Pheromone traps (85% methyl-eugenol, 5% Naled (insecticide), 10% saturated sugar) can be used for trapping male flies (Jackson trap). A three-component attractant (ammonium acetate, putrescine, and trimethylamine lures) may be used to trap female population of Medfly. (No wind: 28 m. range).[http://www.ncbi.nlm.nih.gov/pubmed/21061993] Malathion-bait sprays and Phloxine B (a xanthene dye; D&amp;amp;C Red #28) are equally effective.[http://www.ncbi.nlm.nih.gov/pubmed/11777044]&lt;br /&gt;
Of six commercial food-based attractants, Nulure captured the greatest proportion of females: 74% compared with 51-68%. But Mazoferm E802 (with or without Spinosad) and Torula yeast captured 2.4-2.6 times more females and 3.4-4.0 times more males than Nulure (also more effective than GF-120, Hymlure and Biolure).[http://www.ncbi.nlm.nih.gov/pubmed/24665714]&lt;br /&gt;
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[u]Fopius arisanus (Sonan)[/u]; F. arisanus is a tiny but most effective[http://www.ncbi.nlm.nih.gov/pubmed/12241567] and the most dominant parasitoid wasp. As parasitism increases, fruit fly infestation decreases.[http://www.ncbi.nlm.nih.gov/pubmed/17598524] Of 3 commonly used bait sprays, which all suppress fruit fly populations, Spinosad and Phloxine B bait sprays are less harmful to these wasps than Malathion bait spray. [http://www.ncbi.nlm.nih.gov/pubmed/11561838]   &lt;br /&gt;
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[u]Diachasmimorpha longicaudata[/u]; D. longicaudata (longtailed fruit fly wasp) is the most important parasitoid wasp used as part of integrated pest management programs against fruit flies such as B. invadens and Ceratitis species. They lay one or more (when hosts are scarce) eggs in fruit fly larva.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014816/] Introduction of D. longicaudata poses minimal competitive risk to F. arisanus.[http://www.ncbi.nlm.nih.gov/pubmed/12241567]&lt;br /&gt;
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[u]Oecophylla longinoda[/u]; O. longinoda is a predator weaver ant that may eat whatever insect or bug they may catch, including caterpillars, beetles, honey bees, driver ants (Dorylus nigricans Ill.) and larvae. O. longinoda is a natural enemy of fruit flies as it eats their larvae. This weaver ant is fiercely territorial and will keep other species of ants (like aphids-farming black ants) at bay. The ants continuously patrol the tree that they live in and may also catch egg-laying female fruit flies in the act, and eat them. B. invadens and C. spp numbers on Kent mangoes may be greatly reduced by Oecophylla longinoda.[http://www.ncbi.nlm.nih.gov/pubmed/17598527] &lt;br /&gt;
Weaver ants, however, also feed on honeydew. They may farm a wide range of honeydew-producing Homoptera (bugs with sucking mouthparts, including scales, aphids and mealybugs), but are mostly associated with Coccids (Saissetia; no virus vector). Only when other Coccids are scarce or unavailable, O. longinoda may farm aphids and mealybugs (unarmoured scale insects that are virus vectors). Mainly in small trees and shrubs, the mechanical damage caused by Coccids is related to the size of the attendant ant colony. Dense populations of O. longinoda are supported by interplanting with clove.[http://www.cabdirect.org/abstracts/19540500227.html;jsessionid=36A454B839F0DEEADFA83F99A7206E6D]&lt;br /&gt;
For the protection of their harvested honeydew, these weaver ants weave a translucent silken tent around living leaves. They use the silk produced by their larvae. It is claimed that pheromones produced by the weaver ants repel egglaying fruit flies. A study in Senegal, however, found that pheromones produced by O. longinoda did hardly repel egglaying females of B. invadens.[http://www.ddrn.dk/filer/forum/File/Abstract_Christina_Abel.pdf]&lt;br /&gt;
On the other hand, trees colonized by O. longinoda (in Ghana) had only 6 to 10% fly infestation, compared to 3% fly infestation in trees treated with Cypermethrin plus Dimethoate. (1614 mg per tree)[http://ugspace.ug.edu.gh/handle/123456789/2387] &lt;br /&gt;
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[u]Praying mantids[/u] may also catch and eat fruit flies. Praying mantids are opportunist predators and do not farm any insects or other bugs. Particularly young mantids may not yet be too large to prey on fruit flies (tephredids). The younger and/or smaller the praying mantids are, the smaller the insects (and any other living creature they may catch) they prey on. Praying mantid nymphs initially feed on aphids and fruit flies (drosophila), and as they grow, they skip to larger preys, such as tephritids, and eventually moths and even much bigger preys.&lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option, GF-120 (Dow chemical) may greatly (81-89% after 7-10 weeks) reduce the number of B. invadens pupae per kg mango.[http://www.ncbi.nlm.nih.gov/pubmed/19449630] Or use Dioptries 80% at the rate of 1 L in 450 L water or Malathion 57% at the rate of 0.5 L to 450 L water / acre. Broad-spectrum insecticides are most damaging to fruit flies&amp;#039; natural enemies such as parasitoid wasps.[http://www.ncbi.nlm.nih.gov/pubmed/19886446] &lt;br /&gt;
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==Long-horn Beetles==&lt;br /&gt;
[[Image:Stem_borer.jpg|thumb|left| Stem Borer [http://www.bitterrootrestoration.com/mango/stem-borer-batocera-rufomaculata.html ©]]]&lt;br /&gt;
[[Image:Dastarcus_helophoroides.jpg|thumb|right| D. helophoroides[https://www.ffpri.affrc.go.jp/labs/seibut/bcg/bcg00284.html ©]]]&lt;br /&gt;
[[Image:Batocera_rufomaculata_2.jpg|thumb|left| Batocera rufomaculata [http://agritech.tnau.ac.in/crop_protection/crop_prot_crop_insectpest%20_Mango_pest&amp;amp;disease.html © TNAU]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Batocera_rubus.jpg|thumb|left| Batocera rubus [http://163.20.112.34/~afu/77v.htm © ]]]&lt;br /&gt;
[[Image:Batocera_baby.jpg|thumb|left| baby Batocera [https://www.flickr.com/photos/bondingtool/14291758294 © Samantha - thebondingtool.com]]]&lt;br /&gt;
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* [u]The (red-spotted) Stem Borer (Batocera rufomaculata)[/u], is a large long-horned beetle (up to 5 cm long body). It cuts incisions on the bark and lays its eggs (up to 200) inside the cuts or cracks of the (damaged) tree bark, trunk or cavities (eg exposed roots). The beetles feed on the bark of living trees and eat the green of young shoots. The grub of the stem borer pupates and feeds inside the stem of mango trees (and other fruit trees, including durian, figs, avocado, jackfruit and cashew nuts), creating a tunnel towards the surface (up to 2 cm in diameter), eventually becoming a beetle. Sap (from the tree) and frass (or coarse sawdust, from the larvae) may drip from the holes. Older larvae may also tunnel deeper in the trunk or branches. This causes drying of branches, and the tree may die. The incidence of infestation of trunk borer is highly influenced by maximum temperature. Infestation is highest when temperature and humidity are high.[http://www.ncbi.nlm.nih.gov/pubmed/24498801] For reproduction, it needs to lay its eggs in a stem that is at least 7 cm in diameter. The Stem Borer has been observed in the west coast of Africa, Madagascar, Mauritius, Réunion, Seychelles, Turkey, Israel, Syria, Iraq, Iran, Pakistan, India, Bangladesh, Sri Lanka, China, Nepal, Thailand, Indonesia, Malaysia and the West Indies. Adults may live up to 4 months. Full grown larva may be 8 to 10 cm long, and may live up to a year, causing a lot of damage. Larval and prepupal + pupal stage lasts about 280 and 24-29 days.[http://www.actahort.org/books/787/787_41.htm]&lt;br /&gt;
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* [u]The (yellow-spotted) Mango Longhorned Beetle (Batocera rubus)[/u] is also a wood borer and about equally large as the Stem Borer. Full grown larvae are 6 to 8 cm long. It attacks various fruit trees, including mango, breadfruit and figs, but also bonsay trees, rubber trees and freshly felled timber. Batocera rubus has been observed in Pakistan, India, Bangladesh, China, Cambodia, Indonesia, Malaysia, Phillipines, Thailand, Taiwan and Vietnam.&lt;br /&gt;
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[u]Natural enemies[/u]; Tiny parasitoid wasps such as Oobius agrili sp.n. (1 mm long), Spathius agrili, Avetianella batocera, Tetrastichus planipennisi and Avetianella xystrocerae sp.n (1.5 mm long) have been shown effective against (the larvae of) some specific wood-boring beetles (Agrilus planipennis, Agrilus sexsignatus and Xystrocera globosa)[http://www.emeraldashborer.info/files/Zhang%20et%20al%202005%20Oobius_Leah.pdf][http://www.fs.fed.us/nrs/pubs/other/2013/2013_McManus_FHTET-13-01.pdf]. Batocera rufus (the mango longhorn beetle), is parasitized (its eggs) by  Louricia ovivora, gen. et sp. n.(a moth), and Ooencyrtus batocerae, sp. n.(a wasp) [http://cabdirect.org/abstracts/19360501471.html;jsessionid=A9E2E64A372836E8E24F2B4E97287DC9] Oobius batocera is an opportunistic parasitic wasp that parasitizes various insect eggs, including beetle eggs. [http://www.nhm.ac.uk/research-curation/research/projects/chalcidoids/database/synonyms.dsml?FamilyCode=EE&amp;amp;ValFamTrib=&amp;amp;VALGENUS=Oobius&amp;amp;VALSPECIES=batocerae&amp;amp;VALAUTHOR=(Ferri%E8re)&amp;amp;VALDATE=1936&amp;amp;ValidAuthBracket=false&amp;amp;HOMCODE=0&amp;amp;&amp;amp;listPageURL=listChalcids.dsml%3FSuperfamily%3DChalcidoidea%26Family%3DEncyrtidae%26Genus%3DOobius] &lt;br /&gt;
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There are also various mites that parasitize Batocera longhorned beetles.[http://www.jstor.org/discover/10.2307/3225250?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21103843600301] Tetrapolipus diastocerae n. sp., Tetrapolipus afrobatocerae n. sp., Tetrapolipus ramarajui n. sp. and Tetrapolipus seemani n. sp. are described. Tetrapolipus afrobatocerae has been observed in Africa, Tetrapolipus hunteri in Australia.[http://www.researchgate.net/publication/233099056_The_Genus_Tetrapolipus_(Acari_Podapolipidae)_Parasites_of_Tropical_and_Semitropical_Cerambycidae_(Coleoptera)_New_Distribution_Records_and_Descriptions_of_Four_New_Species] Tetrapolipus sulawesiensis is a mite that parasitizes Batocera herculus.[http://d.wanfangdata.com.cn/periodical_dwfl200202010.aspx] &lt;br /&gt;
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[u]Dastarcus helophoroides[/u]; This predator/parasitoid beetle (originally from China and Japan) is an important natural enemy of longhorned beetles. In the larval stage they attack various long horn beetles, including Batocera horsfieldi and Anoplophora glabripennis. Populations are attracted specifically by the frass of their original host. Eggs are laid twice a year, near the host entrance hole, frass-extrusion holes or larval tunnel walls, guided by semiochemicals. The freshly hatched (at about 21°C) first instars (0.7 mm in length) have legs, and actively move to the host. They bite and paralyze the host, and then suck hemolymph and degenerated tissues from the dead or paralyzed prey. Adults can live over 4 years. [http://link.springer.com/article/10.1007%2Fs10526-009-9224-y#page-1] Mass production is feasible if hatched larvae are fed cerambycid larvae until they are about 8 mm in length, and subsequently reared on artificial diet (silkworm pupa powder, dry yeasts, yeast extract, sucrose, peptone, squid liver oil, preservatives and distilled water). The full grown larvae are about 18 mm in length.[http://link.springer.com/article/10.1023%2FA%3A1009936609401#page-1] Adults don&amp;#039;t readily disperse; they need to be released on each tree separately. And even if you release them at 1.2 m height, their effectiveness decreases with increasing height in the tree. Yet their effectiveness may be very high; up to 85% host mortality. [https://www.ffpri.affrc.go.jp/labs/kanko/401-1.pdf]&lt;br /&gt;
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[u]Control[/u]; The stem borer is active during the night and attracted by light, so that light traps may be effective. Bacillus thuringiensis is a bacterium commonly used as a biological pesticide, and naturally present in the gut of various caterpillars. This bacterium is toxic to many insects. A specific strain of this bacterium, Bacillus thuringiensis ZQ-89 is also toxic to adult long-horned beetles.[http://www.ncbi.nlm.nih.gov/pubmed/21332894] Prevent the stem borer from laying eggs in the bark by applying a thick layer of coaltar on the stem. Or one may paint (at 0.5%) adult mango trees with monocrotophos or phosalone.[http://www.cabdirect.org/abstracts/19790379463.html] One may also specifically apply the insecticides to the potentially affected sites (cracks and cavities), and to twigs and young shoots to deter feeding by adults. One may poke with a hard wire to physically damage, and/or inject insecticides where larvae are suspected. Chloroform, ethyl acetate, Metasystox [demeton-S-methyl] and a mixture of petroleum and kerosene oil (at 5 ml/bore) and ethylene dibromide (at 3 ml/bore) gave 100% mortality of B. rufomaculata larvae.[http://www.cabdirect.org/abstracts/19881107545.html] Plaster the holes with wet clay, aluminium phosphide tablets (3 g/hole) and dichlorvos (0.1% spray).[http://www.cabdirect.org/abstracts/19790379463.html] Among the chemical treatments, Imidacloprid 17.8% SL, Thiamethoxame 25% WG was found best in management of mango stem borer.[http://www.gifre.org/admin/papers/gjbahs/management-vol-2-4-gjbahs.pdf]&lt;br /&gt;
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==Mango Hoppers==&lt;br /&gt;
[[Image:Idioscopus_niveosparsus.jpg|thumb|left| I. niveosparsus [http://163.20.112.34/~afu/641x.htm ©]]]&lt;br /&gt;
[[Image:Mallada_basalis.jpg|thumb|right| Mallada sp. [http://3insect.blogspot.nl/2012/06/mallada-basalis.html © Chen KunTsan]]]&lt;br /&gt;
[[Image:Idioscopus_clypealis.jpg|thumb|left| I. clypealis [http://163.20.112.34/~afu/641y.htm ©]]]&lt;br /&gt;
[[Image:Mallada_signata_3.jpg|thumb|right| Mallada signata [http://www.oocities.org/brisbane_lacewings/Chrysopidae.htm © Keith Power]]]&lt;br /&gt;
[[Image:Idioscopus_nitidilus.jpg|thumb|left| I. nitidilus [http://tech.groups.yahoo.com/group/pestnet/message/6414 ©]]]&lt;br /&gt;
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Mango hoppers:&lt;br /&gt;
* Idioscoynio chypeabis&lt;br /&gt;
* Idioscopus niveosparsus&lt;br /&gt;
* Idioscopus clypealis&lt;br /&gt;
* Idioscopus nitidulus&lt;br /&gt;
* Amritodus atkinsoni aka mango leafhopper&lt;br /&gt;
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Mango hopper nymphs as well as adults puncture and suck the sap of tender parts of the mango plant. Heavy drainage will cause the leaves to curl and dry. Infested flowers will shrivel and turn brown. Hoppers also secrete honeydew, which may cause sooty mould, inhibiting the leaves from obtaining energy from daylight. Sufficient spacing of trees and pruning of overlapping branches are essential in preventing hopper infestation, as shade (and high humidity) favour hopper multiplication.&lt;br /&gt;
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[u]Natural enemies[/u]:&lt;br /&gt;
Parasites: Aprostocetus sp., Gonatocerus sp. and Polynema sp. parasitize eggs of Idioscopus clypealis and Idioscopus nitidulus.[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
A preparation of the fungus Beauveria bassiana is also somewhat effective against mango hoppers. Chrysopa lacciperda [Plesiochrysa lacciperda] and Mallada boninensis predate on on nymphs of I. clypealis, I. nitidulus and Amritodus atkinsoni.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9]&lt;br /&gt;
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[u]Mallada boninensis[/u]; This green lacewing is an effective [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=5&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFQQFjAE&amp;amp;url=http%3A%2F%2Fwww.researchjournal.co.in%2Fonline%2FIJAS%2FIJAS%25203(2)%2F3_A-164-166.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNGZ9IidKvjO6s1zNs0HvbUMpKKPBg] generalist predator, feeding on mealy bugs (Mani and Krishnamoorthi, 1987), white flies (Selvakumaran et aI., 1996), bollworms and aphids (Kabissa et al., 1996) and on nymphs of Aphis gossypii, Aphis craccivora and Rhopalosiphum maidi [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=3&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEAQFjAC&amp;amp;url=http%3A%2F%2Fwww.ets-staffing.com%2Fcurrentbiotica%2Fjournals6-issueiii%2Fcb-6-3-short-notes-3.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNETNWfEOrZznoJjj0ZrFFOINJ2liw], blackflies, psylla and leaf miner. Green lacewings are recommended for the integrated pest management programme (Nehare et al., 2004). M. boninensis feeds well on Corcyra cephalonica eggs[http://cabdirect.org/abstracts/20113350526.html;jsessionid=69EA3BE15508EF9C029CE688C342FD4D] (laboratory host) C. cephalonica can be mass reared on Jowar along with groundnut, streptomycin, vitamin complex, Na and K salts.&lt;br /&gt;
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[u]Control[/u]: &lt;br /&gt;
Spray Imidacloprid (0.005%; 0.3 ml/L water), acephate 75SP (1.5 g/L water), Etofenprox (0.03%), Phosalone (1.5 ml/L water), Carbaryl (0.15%; 3 g/L water), Monocrotophos (0.04%), Phosphamidon (0.05%) or Methyl Parathion (0.05%). &lt;br /&gt;
* Spray 3 times; First spray should be given during the early stage of flower formation. The second spray should be given at a flower size of 6 to 8 cm, still before blooming. The third spray should be given after fruit setting, when the fruits are the size of a pea. Or:&lt;br /&gt;
* First spray should be given during the early stage of flower formation. The second spray should be given 2 weeks later. Or:&lt;br /&gt;
* Spray 3 times; First spray of imidacloprid (0.005%; 0.3 ml/L water) should be given during the early stage of flower formation. The second spray, thiamethoxam (0.005%; 0.2 g./L water) or acephate 75SP (1.5 g/L water) should be given at after fruit setting. If hopper infestation persists, the third spray, of carbaryl (0.15%; 3 g/L water) should be given prior to fruit maturation.&lt;br /&gt;
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Imidacloprid residues persist in peel for 60 days and in pulp for 50 days. Mature Dashehari fruits at harvest (after 85 days of spraying) were free from imidacloprid residues.[http://www.ncbi.nlm.nih.gov/pubmed/23196371]&lt;br /&gt;
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==Mango Midges==&lt;br /&gt;
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[u]Inflorescence Midge[/u]&lt;br /&gt;
[[Image:Platygaster_sp.jpg|thumb|right| Platygaster sp. [http://liuzhen.000space.com/insect/organism.php?id=1367&amp;amp;type=list]]]&lt;br /&gt;
[[Image:Inflorescence_midge.jpg|thumb|left| [http://www.bitterrootrestoration.com/mango/inflorescence-midge-erosomyia-indica.html Erosomyia indica ©]]]&lt;br /&gt;
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This midge (Erosomyia indica) lays eggs in new inflorescence (preventing flower and fruit set) and new leaves around it, and then the larvae will eat their way through. The maggots penetrate and feed on the tender parts of the plant, such as shoots, buds and flower buds. The flowers will dry and fall off. For pupation, the mature larvae will drop into the soil. The adult midge lives only one day and doesn&amp;#039;t cause any damage. Maggots from eggs laid on new fruits will eat their way into the fruit, which will turn yellow and drop.[http://www.actahort.org/books/231/231_15.htm] The inflorescence midge is an important pest in India in particular.&lt;br /&gt;
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Infloresence midge is parasitized by Platygaster sp., Eupelmus sp. and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19881115612.html;jsessionid=E4CDBFFBEE75C82EDA1BA2CA7B5F1DD2], which all also parasitize Gall Midges. The inflorescence midge is also parasitized by Aprostocetus sp.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9] and Mirufens longifunculata. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false]&lt;br /&gt;
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Another mango midge, Procystiphora mangiferae is parasitized by Pirene sp. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], which also parasitizes mango gall midges.&lt;br /&gt;
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Chemicals: If you have no other option, Parathion, Dimethoate, Phosphamidon and Endosulfan much reduced infestations on mango shoots.[http://www.cabdirect.org/abstracts/19740517480.html;jsessionid=59B14F930CB8EDFD2F1B50F1B04C7692] The most effective insecticide for control appeared to be phosphamidon (Dimecron), whether mixed with diazinon or not, as a foliar spray. [http://www.cabi.org/isc/abstract/19770549886]&lt;br /&gt;
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[u]Gall midges[/u]&lt;br /&gt;
[[Image:Mango_galls.jpg|thumb|left| [http://www.infonet-biovision.org/default/ct/124/crops © A.M. Varela]]]&lt;br /&gt;
[[Image:Eupelmus_urozonus.jpg|thumb|right| Eupelmus urozonus [http://www.biolib.cz/en/image/id150459/ © Miroslav Fiala]]]&lt;br /&gt;
[[Image:Mango_galls_P_matteiana.jpg|thumb|left| P. matteiana galls [http://www.pests.blogfa.com/8907.aspx © RJ Gagné]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Gall_midget_exit_holes.jpg|thumb|left| Exit holes [http://biostor.org/reference/55262 © RJ Gagné]]]&lt;br /&gt;
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There are various mango gall flies:&lt;br /&gt;
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- Erosomyia mangifereae or Procontarinia frugivora has been observed in the Philippines, India, West Indies and Brazil.&lt;br /&gt;
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- Asynapta sp. in West Indies.&lt;br /&gt;
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- Dasyneura mangifereae in Hawaii.&lt;br /&gt;
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- P. allahabadensis, P. biharana, P. brunneigallicola, P. viridigallicola, P. echinogalliperda, P. keshopurensis, P. mangifoliae, P. tenuispatha, P. amraeomyia and Dasyneura amaramanjarae have been observed in India (and some in Pakistan).&lt;br /&gt;
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- P. mangicola in China and Japan.&lt;br /&gt;
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- P. matteiana (1.5 mm long) in India, Kenya, Mauritius, Java and Réunion[http://biostor.org/reference/55262][http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=4&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFEQFjAD&amp;amp;url=http%3A%2F%2Fwww.geocities.ws%2Fmdce2005%2FMDCE2005%2F27-98_102.pdf&amp;amp;ei=HS5zU-P3DomXyQOd_IHgCA&amp;amp;usg=AFQjCNHlZtUS8qYFE2lCVSqh3ow0K4ukLg&amp;amp;sig2=vW-1UFdjzeE3l58kmc8eMg&amp;amp;bvm=bv.66699033,d.bGQ]. &lt;br /&gt;
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The tiny (1-2 mm) mango gall flies lay their eggs on the surface of young leaves, buds, shoots and flowers. After hatching, the maggots will eat their way into the plant tissue. This will show as galls (3-4mm) on the leaves. Once mature, they drop to the ground to pupate. The holes that they left behind are susceptible to fungal infections. Infested fruits initially show small (1mm) brownish lesions, which grow as the fruit grows. In young fruits, the exit holes are usually near the point of attachment, on the bottom side. Most infested fruits fall to the ground before ripening. Optimal conditions for reproduction is 26°C and a relative humidity of 70 to 80%. Infestation particularly occurs at bud-burst stage, at fruit set and on tender leaves of new flushes.[http://openagricola.nal.usda.gov/Record/IND92003672]&lt;br /&gt;
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[u]Natural enemies[/u]; Parasitic insects such as Platygaster sp., Eupelmus sp., Tetrastichus sp., Systasis dasyneurae [http://www.cabdirect.org/abstracts/19881115612.html], Pirene sp. and the predator ants Formica rufa (aka red wood ant, native to Europe and North America), Oecophylla longinoda (weaver ant native to West and Central Africa) and Oecophylla smaragdina (native to South-east Asia) and Camponotus sp. (aka carpenter ant, native to forests world wide).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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[u]Baits[/u]; Coriander oil attracts Erosomyia. D. amaramanjarae is attracted by a mixture of glycine, sodium hydroxide, ammonium carbonate and strong ammonia solution. Bordeaux mixture is a repellent. &lt;br /&gt;
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[u]Chemicals[/u]; Various systemic insecticides are equally effective: Dimecron (phosphamidon), Anthio (formothion) and Metasystox (demeton-S-methyl). Most effective is a mixture of phosphamidon (0.03%) and diazinon (0.03%).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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==Mango Moths==&lt;br /&gt;
[[Image:Indarbela_quadrinotata.jpg|thumb|left| Indarbela quadrinotata [http://www.agriplaza.in/orange-protection.htm#indarbela © Agriplaza]]]&lt;br /&gt;
[[Image:Podagrionella_habitus.jpg|thumb|right| Podagrionella [http://www.waspweb.org/Chalcidoidea/Torymidae/Toryminae/Podagrionini/Podagrionella/index.htm © S. van Noort]]]&lt;br /&gt;
[[Image:Indarbela_quadrinotata_2.jpg|thumb|left| Indarbela quadrinotata [http://www.ku.ac.th/e-magazine/september43/bark_feeders/ © KUAC]]]&lt;br /&gt;
[[Image:Mantis_religiosa_5.jpg|thumb|right| Mantid eating large moth [http://www.agefotostock.com/en/Stock-Images/Rights-Managed/K60-589612 © Oriol Cabrero]]]&lt;br /&gt;
[[Image:Eudocima_homaena_2.jpg|thumb|left| Eudocima homaena [http://gaga.biodiv.tw/9702bx/049.htm © GaGa]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Eudocima_homaena_4.jpg|thumb|left| Eudocima homaena [http://blog.xuite.net/mbbrgs/twblog1/137791571-%E9%91%B2%E8%90%BD%E8%91%89%E5%A4%9C%E8%9B%BE++Eudocima+homaena++(Hubner,+1823)]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Eudocima_homaena_1.jpg|thumb|left| Eudocima homaena [http://yunol.com.tw/gallery3/index.php/insect/Eudocima-homaena]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-l5.php © PathPiva]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella_2.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-1.php © PathPiva]]]&lt;br /&gt;
[[Image:Orthaga_exvinacea.jpg|thumb|left| Orthaga exvinacea [http://www.nbaii.res.in/insectpests/images/Orthaga-exvinacea7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Orthaga_euadrusalis.jpg|thumb|left| Orthaga euadrusalis [http://www.jpmoth.org/~dmoth/62_Pyralidae/6003_Epipaschiinae/60031001_Orthaga_euadrusalis_1865/Orthaga%20eudrusalis%200208287801.jpg]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_2.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42845]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42848]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_3.jpg|thumb|left| Citripestis eutraphera [http://www.boldsystems.org/index.php/Taxbrowser_Taxonpage?taxid=375866 © ANIC/BIO]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_2.jpg|thumb|left| P. jocosatrix [http://www.nbaii.res.in/insectpests/images/Penicillaria-jocosatrix7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Aleiodes_indiscretus.jpg|thumb|right| Aleiodes indiscretus [http://commons.wikimedia.org/wiki/File:Aleiodes_indiscretus_wasp_parasitizing_gypsy_moth_caterpillar.jpg © Agricultural Research Service]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_3.jpg|thumb|left| P. jocosatrix [http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar © DAFF]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix.jpg|thumb|left| P. jocosatrix [http://www.flickr.com/photos/bettaman/3973171117/ ©]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Caterpillar.jpg|thumb|left| Deanolis sublimbalis [http://www.padil.gov.au/pests-and-diseases/pest/main/136263/43328 © S. Eyres]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Moth.jpg|thumb|left| Deanolis sublimbalis [http://www.ces.csiro.au/aicn/name_s/b_1295.htm © DAFF]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Chlumetia_transversa_2.jpg|thumb|left| C. transversa [http://www.nbaii.res.in/insectpests/Chlumetia-transversa.php © NBAII]]]&lt;br /&gt;
[[Image:Chlumetia_transversa.jpg|thumb|left| C. transversa [http://www.inaturalist.org/observations/572035 © T Bonebrake]]]&lt;br /&gt;
[[Image:Goryphus_basilaris.jpg|thumb|right| Goryphus basilaris [http://www.hkwildlife.net/viewthread.php?tid=49119 © Daydream]]]&lt;br /&gt;
[[Image:Parasa_lepida.jpg|thumb|left| P. lepida [http://fanseab.exblog.jp/9707580]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Parasa_lepida_2.jpg|thumb|left| P. lepida [http://www.isan.clubs.chula.ac.th/para_norkhai/?transaction=post_view.php&amp;amp;cat_main=all&amp;amp;id_main=301&amp;amp;star=270&amp;amp;pg=28 © ปิ่นลม]]]&lt;br /&gt;
[[Image:Parus_major.jpg|thumb|right| Parus major [http://en.wikipedia.org/wiki/Parus_major#mediaviewer/File:Parus_major_2_Luc_Viatour.jpg © Luc Viatour / www.Lucnix.be]]]&lt;br /&gt;
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[u]Bark Eating Caterpillar[/u]&lt;br /&gt;
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The Bark Eating Caterpillar (&amp;#039;&amp;#039;Indarbela quadrinotata&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Indarbela tetraonis&amp;#039;&amp;#039;) aka Mango Bark Feeder has been observed in India, Bangladesh and Sri Lanka. The moth has a wingspan of maximally 2 cm. This wood boring insect may attack a wide variety of trees, including mango, guava, jujube, pomegranate and apple trees. In plantations, infestation generally starts with the onset of premonsoon rains. The main symptom is plastered dark-brown masses on tree trunks and large branches. These webbed masses consist of chewed on wood remainders / frass, excrements and silken thread. Eggs are laid in clusters on the bark of trees. Initially, the newly hatched larvae occur in groups browsing on the bark. Then they migrate and lodge in shoots and buds. As the larvae grow, they start tunneling into the wood, up to 25 cm deep. This tunnel is kept free of frass. The frass and excreta from the tunnels are used for constructing the extended tunnel mouth (sleeve). As the larvae get bigger, they make the tunnel wider. The larvae hide in these tunnels during the day and come out at night to eat the surrounding bark of the tree. The rate of feeding is faster during the hottest months. The larva minimally eats 16 mg / day, at 12.5 degrees Celsius. The maximum is 166 mg/day at 35 degrees Celsius. Similarly, food consumption is maximum at 96% RH and minimum at 56% RH.&lt;br /&gt;
When large patches of bark are eaten away, the tree gets increasingly exposed to various bacteria, fungi etc. And if substantial areas of bark have been eaten, the tree will eventually die. Prolonged water stress decreases the resistance of the host tree. The larvae pupate inside the tunnel and the emerging moth leaves its &amp;quot;pupal skin&amp;quot; at the mouth of the borer hole. The pupa is 1.5 cm long. The larval stage lasts for about 8 months. The pupal period lasts for about 9 days. It has a little more than one generation per year.  [http://www.tropecol.com/pdf/open/PDF_49_1/09%20Shashidharan.pdf]&lt;br /&gt;
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Natural enemies; Two fungi; Aspergillus candidus and Beauveria bassiana have been reported to cause mortality of the Bark Eating Caterpillar in the field. Laboratory trials have indicated 100% larval mortality by these fungi. The Bark Eating Caterpillar is parasitized by Podagrionella indarbelae (&amp;#039;metallic&amp;#039; parasitic wasps), which may be fed the eggs of I. tetraonis.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf] Podagrionella is found in tropical African countries, Senegal, Algeria, Malawi, France, Spain, India, Thailand, Indonesia, The Philippines, Australia&lt;br /&gt;
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Control; prevent overcrowding by sufficient spacing in between trees. One may insert a sharp metallic probe/spike into each tunnel/hole to kill the larvae. One may seal the tunnel entrance using tar or wax. Of the various insecticides screened against this insect, monocrotophos (0.1 %), quinalphos (0.1 %) and fenvalerate (0.08%) gave best results.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf]&lt;br /&gt;
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[u]Fruit-piercing Moths[/u] &lt;br /&gt;
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&amp;#039;&amp;#039;Eudocima&amp;#039;&amp;#039; (aka Othreis) are very large moths (up to 10 cm wingspan) with orange abdomen. Adult moths have special sclerotised structures at the tip of their proboscis with which they are&lt;br /&gt;
capable of drilling a hole through the hard rind of fruits, and suck out the juices.[http://www.jbiopest.com/users/LW8/efiles/Ramkumar_V32.pdf] The resulting wound is used by various bugs and diseases to attack the fruit (including mango, banana, grapes, orange, clementine, kiwi, lychee, pineapple, peach, apricot, papaya apple and pear). Eudocima moths look like dead leaves when in hiding mode. Until they show their bright orange hindwings with broad black margins and a large black spot in the middle. Mature larvae are 4 to 5 cm long. These moths have been observed in virtually any tropical country, except for the Americas. The adults fly (very well), feed and mate during the night.  &lt;br /&gt;
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The most widespread Eudocima specie is [http://www.waiwiki.org/index.php?title=Eudocima#Eudocima_phalonia &amp;#039;&amp;#039;Eudocima fullonia&amp;#039;&amp;#039;] (aka Eudocima phalonia)[http://www.hindawi.com/journals/tswj/2011/753484/], followed by [http://www.waiwiki.org/index.php?title=Eudocima#Eudocima_materna &amp;#039;&amp;#039;Eudocima materna&amp;#039;&amp;#039;] and &amp;#039;&amp;#039;Eudocima homaena&amp;#039;&amp;#039;.&lt;br /&gt;
Eudocima homaena occurs in Costa Rica, Thailand, India, Sri Lanka, Hong Kong, China [http://www.gbif.org/occurrence/search?taxon_key=6132915&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false] and Chinese Taipei [http://www.gbif.org/occurrence/search?taxon_key=1772634&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=0]. Eudocima larvae do not feed on mangoes, nor the mango trees. Instead, the larvae feed on very specific Fabaceae and Menispermaceae. Variation in the alkaloids associated with certain menisperm genera (eg quaternary alkaloids in Tinospora[https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2007-969933]) may explain the very specific (or even exclusive) moth–host plant relationships.[http://www.publish.csiro.au/?paper=SB9960227] In Australia the larvae of Eudocima salaminia feed almost exclusively on the forest vine, Stephania japonica.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563]&lt;br /&gt;
Eudocima homaena larva may feed on Cocculus hirsutus, Tinospora acuminata, Diploclisia glaucescens [http://www.jbiopest.com/users/LW8/efiles/Ramkumar_V32.pdf], Achyranthes cocculus, Cissampelas pareira [http://www.aapmhe.in/index.php/pmhe/article/download/42/41], and on specific Cyclea (not Cyclea peltata[http://www.aapmhe.in/index.php/pmhe/article/download/42/41]), Menispermum and Tiliacora species [http://www.en.inforapid.org/index/index.php?search=Cocculus], but not on other Tinospora species, Stephania species, nor on Erythrina indica.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41]&lt;br /&gt;
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Control; Regularly check the orchard during the night using a flashlight. The adult Eudocima moths have large eyes that glow red when illuminated. Adult [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] may eat large moths such as Fruit-piercing moths. The female Praying mantis lays a few hundred eggs in a cluster (ootheca) attached to an object like a branch or trunk. Large species of mantids may have a length of up to 12 cm. Their colour is usually adapted to the environment they naturally live in. They have about 2 generations per year. Young mantids look like adults, but don&amp;#039;t have wings. Adult females often have no wings either, or reduced wings.&lt;br /&gt;
Eudocima are parasitized by Euplectrus [http://link.springer.com/article/10.1023/B:BICO.0000036439.74117.2f#page-1][http://www.aapmhe.in/index.php/pmhe/article/view/42] and Trichogramma.[http://images.peabody.yale.edu/lepsoc/jls/2010s/2011/2011-65-1-053.pdf] Bats may be major predators.[http://www.cabdirect.org/abstracts/19370500524.html]&lt;br /&gt;
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[u]Honeydew Moth[/u]&lt;br /&gt;
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The Honeydew Moth (&amp;#039;&amp;#039;Cryptoblabes gnidiella&amp;#039;&amp;#039; aka Albinia casazzar aka Albinia wockiana) aka Christmasberry Moth survives only in warm climates (all over the world). It attacks a large variety of crops, including mangoes, banana, oranges, clementines, grapes, loquats, pomegranates, avocado, coffee, maize and rice. The caterpillars (1 cm long) mainly feed superficially on fruits, but may also feed on leaves, flower, shoots and bark. The newly hatched larvae and the adults, however, solely feed on honeydew. The adults are active during the night. Females mate 1 to 4 times during their entire life. One Honeydew Moth may lay up to 100 eggs within 2 days after mating, on fruit or leaves (averagely 150 in total, during lifetime). These eggs hatch within one week (or longer, in colder regions). It has several generations per year, depending on the climate. Adult moths are good flyers (1 to 2 cm wingspan) and may rapidly spread to poorly maintained orchards (infested with honeydew).&lt;br /&gt;
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Natural enemies; Scolothrips sexmaculatus and Orius spp. prey on the eggs and early-instar larvae. The Honeydew Moth is parasitized by Phanerotoma sp.[http://www.cabdirect.org/abstracts/19740514736.html] and Trichogramma Platneri (both parasitoid wasps).&lt;br /&gt;
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Trichogramma are extremely tiny wasps (&amp;lt; 0.5 mm). They insert their eggs into the eggs of the moth. The larvae feed, grow and pupate inside the host egg, which converts the colour of the host egg from white to black. The emerging wasp eats its way out and is ready to mate. The eggs from unmated females will produce only males, whereas the eggs from mated females will produce both males and females. Adult Trichogramma feed on nectar from flowers. One female wasp may parasitize 50 moth eggs during her life. The optimum conditions for Trichogramma are 20 to 27 degrees Celsius and 60% RH. Most insecticides kill Trichogramma.&lt;br /&gt;
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Control; This moth is attracted by honeydew secreted by aphids, scale insects and other honeydew secreting bugs. It is therefore most effectively controlled by controlling all honeydew secreting bugs. Its instars are highly susceptible to Bacillus thuringiensis.&lt;br /&gt;
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[u]Leaf Webber[/u]&lt;br /&gt;
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These brown moths (&amp;#039;&amp;#039;Orthaga euadrusalis&amp;#039;&amp;#039; and &amp;#039;&amp;#039;Orthaga exvinacea&amp;#039;&amp;#039;) are a minor mango (and Cashew nut) pest. They have been observed in India and Indonesia. Females mate only one time during their whole life.[http://www.ncbi.nlm.nih.gov/pubmed/20136014] The leaf webber lays eggs on young leaves and buds and creates a web around pods, leaves and flowers for its larvae to pupate in. Such a webbed cluster may contain several larvae. The Leaf Webber causes relatively very little damage, as the larvae merely scrape and eat from the surface of the leaves.&lt;br /&gt;
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Control: Application of Serratia marcescens (a bacterium), Aspergillus flavus (a fungus) and Beauveria bassiana (a fungus) are all very effective.[http://www.cabdirect.org/abstracts/19810586732.html] The leaf webber is parasitized by Brachymeria lasus, Hormius sp., Pediobius bruchicida and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html] and also by Trichogramma chilonis and Trichogramma pretiosum.[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=37933]&lt;br /&gt;
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[u]Mango Fruit Borer[/u];&lt;br /&gt;
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The Mango fruit borer (&amp;#039;&amp;#039;Citripestis eutraphera&amp;#039;&amp;#039;; a moth) has been observed in Indonesia, India and Australia. Its lays its eggs (&amp;lt; 1 mm) on fruit or stalk. The larvae feed on mango pulp (and cashews) and cause premature fruit drop, mainly in young fruit. The larvae hatch in 2 days. They are initially white, then red-violet when young, turning to dark blue as they grow. Hatched larvae initially feed on the peel of the fruit. Then they bore into into the fruit and feed for 12 to 15 days before they pupate in the fallen fruit, or in the soil. Often there is more than one larva in a fruit with a total of 15 recorded in one fruit.[http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42846].&lt;br /&gt;
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Control: Its natural enemies are parasitoid wasps (Aleiodes sp. and Euplectrus sp.) and a parasitoid fly (Blepharella lateralis).&lt;br /&gt;
[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=9416] To protect the stems, cover them with a cloth or Jute and paste charcoal over it. Fostoxin tablets can also be placed and sealed in the holes made by the borers.&lt;br /&gt;
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[u]Mango Shoot Borer[/u]&lt;br /&gt;
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Mango Shoot Borer (&amp;#039;&amp;#039;Penicillaria jocosatrix&amp;#039;&amp;#039;) or Velvet mango slug; P. jocosatrix (wingspan 2 - 2.5 cm) has been observed in Indonesia, The Philippines, Malaysia, Vietnam, Guam, Hawaii, Thailand and Australia. Its larvae; the caterpillar of this moth (up to 3 cm long) causes damage to shoots, stems, inflorescence, flowers and fruits of the mango tree. Pestalotiopsis anacardiacearum sp. nov. (fungal leaf rot) is found on dead mango leaves associated with P. jocosatrix (the Mango Shoot Borer).[http://biotaxa.org/Phytotaxa/article/view/phytotaxa.99.2.1] Eggs hatch in 3-5 days. Larval development takes 8-10 days. Mature larvae pupate in the soil and the moth emerges 16-20 days later.[http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar]&lt;br /&gt;
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Natural enemies: Euplectrus sp. (a parasitoid wasp) and Blepharella lateralis (a parasitoid fly) may effectively decrease Mango shoot borer infestation [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], and are also effective against the Mango fruit borer.&lt;br /&gt;
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[u]Mango Seed Borer[/u]&lt;br /&gt;
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The Mango Seed Borer (&amp;#039;&amp;#039;Deanolis sublimbalis&amp;#039;&amp;#039; aka &amp;#039;&amp;#039;Autocharis albizonalis&amp;#039;&amp;#039;) is the Red banded mango caterpillar. It is a mango pest in Australia, Burma, The Philippines, Malaysia, Vietnam, China, Indonesia and Papua New Guinea. Severe infestation may cause up to 50% yield reduction. This grey snout moth (wingspan 2 cm) lays its eggs on the top of the mango. Its larvae (one or more per fruit) develop within the fruit in 2 to 3 weeks. Initially they feed on the pulp, and then on the seed. The fruit will split, rot and drop. The mature larvae will pupate in the soil.&lt;br /&gt;
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Natural enemies: Rychium attrisimum are wasps that feed on the larvae of the Mango Seed Borer as they leave the fruit.[http://www.planthealthaustralia.com.au/wp-content/uploads/2013/03/Red-banded-mango-caterpillar-CP.pdf] Trichogramma chilonis and Trichogramma chilotreae are tiny parasitoid wasps that parasitize the eggs of the Mango Seed Borer and hundreds of other moths. They are so small that with the naked eye you cannot see what they are. They have a wingspan of 0.5 mm. They live about 2 weeks, and may lay several eggs in a single moth egg.&lt;br /&gt;
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Chemicals: If you have no other option, Deltamethrin and Cyfluthrin are most effective.&lt;br /&gt;
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[u]Mango Tip Borer[/u]&lt;br /&gt;
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Mango Tip / Shoot / Bark / Trunk / Twig Borer (&amp;#039;&amp;#039;Chlumetia transversa&amp;#039;&amp;#039;) aka aka Shoot Boring Caterpillar is a serious mango (and litchi) pest. The major symptom is drying of the tip of young shoots. Female moths lay their eggs on tender leaves. Newly hatched larvae bore into these leaves. As they grow they bore into young shoots near the growing point, and subsequently tunnel down. The leaves of affected shoots will droop. It has four generations each year, and in colder regions overwinters as pupae in young branches and bark. Generations are overlapped.[http://europepmc.org/abstract/CBA/367089] Full grown caterpillars are 2 to 2.5 cm long.&lt;br /&gt;
C. transversa has been observed in Indonesia, The Philippines, Malaysia, Thailand, Taiwan, China, India, Pakistan, Sri Lanka and Australia. &lt;br /&gt;
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The Mango Tip Borer is parasitised by Bracon greeni, Meteorus sp. and Goryphus sp [http://www.cabdirect.org/abstracts/19810586735.html], which are all parasitoid wasps. Its larvae are also parasitized by the fly Megaselia chlumetiae sp. nov. Its larvae enter the host and develop by consuming the host&amp;#039;s internal tissues. Pupariation normally takes place within the host&amp;#039;s empty skin.[http://www.cabdirect.org/abstracts/19921163681.html]&lt;br /&gt;
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Control; If you have no other option, spraying 2.5% deltamethrin EC for three times may be most effective (87% control efficiency) and the least harmful to the tree. Four insecticides tested; 80% dichlorvos EC(dilution of 1250), 47.5% chlorpyrifos EC(dilution of 1000), 2.5% deltamethrin EC (dilution of 2500) and 2% abamectin EC(dilution of 2000) were applied on mango trees during 2 months. Second best was 2% abamectin EC at a dilution of 2000. The other two insecticides were less effective, which might be due to the development of resistance in pests against these insecticides on account of longer repeated applications.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GXNY200910010.htm]&lt;br /&gt;
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[u]Nettle Caterpillar[/u]&lt;br /&gt;
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The Nettle Caterpillar, aka the Blue-striped Nettle Grub (&amp;#039;&amp;#039;Parasa lepida&amp;#039;&amp;#039; aka Latoia lepida; 2 to 3 cm long) has been observed in China, Pakistan, India, Sri Lanka, Bangladesh, Vietnam, Thailand, Malaysia, Japan and especially Indonesia. Parasa lepida may infest various fruit trees, including mango, cherry, persimmon, Indian almond and rose apple, and also coffee, tea, cocoa and palms, with a preference for coconut trees. The caterpillar of this species have spines containing a poison that causes serious irritation and inflammation in humans upon contact. It feeds on the leaves and shoots of the mango tree. Initially, defoliation will be localized, affecting only one or a few trees. Thus the infestation is easily identified. Subsequently, as new generations of this pest emerge, the infested area may rapidly expand. The female moth lays eggs on mango leaves. The cocoons are laid side by side. The cocoons are so hard and stout that they remain on the trunks for 5 to 6 years after spinning. Cocoons from which adults have successfully emerged have clear emergence holes. Newly hatched caterpillars will feed on the underside of the epidermis, stripping it off the leaflets. They often begin where the eggs were laid; at the tip. Then they eat the edges of the leaflet and eat large areas of the lamina. When they have finished developing, the whole leaflet will have been consumed systematically from tip to base, leaving only the midrib. On this midrib, the notched indentations are the only visible remainders left by the caterpillars. [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=38935]&lt;br /&gt;
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Parasa lepida is predated on by Agriosphodrus dohrni (The Assassin Bug, native to China, India, Indonesia). This 2 cm long Assasin Bug may be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
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The great tit (Parus major) is a predator of Parasa lepida cocoons in Japan. The most effective parasites are Apanteles parasae (a parasitoid wasp) and Chaetexorista javana (a parasitoid fly).[http://www.cabdirect.org/abstracts/19820596771.html]&lt;br /&gt;
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Salt spray from the sea and the resulting plant stress (considerable damage) may negatively affect the Parasa lepida moth.[http://www.bioone.org/doi/pdf/10.3956/0031-0603-83.3.193]&lt;br /&gt;
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==Mango Scales==&lt;br /&gt;
[[Image:Coccus_viridis.jpg|thumb|left| Coccus viridis [http://www.insectimages.org/browse/detail.cfm?imgnum=5385208 © J.W. Lotz]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| M. signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
[[Image:Coccus_hesperidum.jpg|thumb|left| Coccus hesperidum [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/09Cocc_hesperidum/4hesperidumHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Ceroplastes_floridensis.jpg|thumb|left| Ceroplastes floridensis [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/03Cero_floridensis/4floridensisHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Chilocorus_kuwanae.jpg|thumb|right| C. kuwanae [http://animal.memozee.com/view.php?tid=2&amp;amp;did=10472 © Jinsuk Kim]]]&lt;br /&gt;
[[Image:Aulacaspis_tubercularis.jpg|thumb|left| Aulacaspis tubercularis[http://gaga.biodiv.tw/new23/9409/076.htm]]]&lt;br /&gt;
[[Image:Azya_orbigera.jpg|thumb|right| A. orbigera [http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php © E Arcaya]]]&lt;br /&gt;
[[Image:Drosicha_mangiferae.jpg|thumb|left| D. mangiferae [http://www.jugantor.com/last-page/2014/04/19/89802 ©]]]&lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri.jpg|thumb|right| C. montrouzieri[http://www.ozanimals.com/Insect/Mealybug-Ladybird/Cryptolaemus/montrouzieri.html ©]]]&lt;br /&gt;
[[Image:Rastrococcus_iceryoides.jpg|thumb|left| R. iceryoides [http://www.nbaii.res.in/insectpests/Rastrococcus-iceryoides.php © NBAII]]] &lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri_larva.jpg|thumb|right| C. montrouzieri larva [http://www.insectimages.org/ © J.W. Lotz, FDACS Bugwood.org]]]&lt;br /&gt;
[[Image:Paracoccus_marginatus.jpg|thumb|left| Papaya mealybug [http://www.ablturismo.com/avispa-enviada-por-correo-salva-cultivos-en-la-india/ ©]]]&lt;br /&gt;
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The main mango scales are:&lt;br /&gt;
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* Coccids ; Coccus viridus (soft green scales) and Coccus hesperidum (soft brown scales)&lt;br /&gt;
* wax scales ; Ceroplastes spp.&lt;br /&gt;
* armoured scales ; Aulacaspis tubercularis, Chloropulvinaria polygonata (the Mango Green Shield Scale) and Aspidiotus destructor.&lt;br /&gt;
* Mealybugs (pseudococcidae) ; Drosicha mangiferae, Rastrococcus iceryoides (&amp;quot;Mango mealybugs&amp;quot;) and Paracoccus marginatus (&amp;quot;Papaya mealybug&amp;quot;, which is also a mango pest)&lt;br /&gt;
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Mango scales suck the sap from the leaves, and as a result, the leaves start drying. This may cause branches to die, blemished fruits and premature dropping. Scales are small insects (1 to 7 mm), generally immobile; as if shells are glued to the plant. Only the newly hatched crawlers (emerging from under a big scale) move to their feeding site. Soft scales produce honeydew, which may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould]. Armoured scales don&amp;#039;t excrete honeydew. The papaya mealybug has been observed in Mexico, Florida, Antigua, Cuba, Dominican Republic, Guadeloupe, Haiti, St Kitts, Martinique, Puerto Rico, St Martin, St Barthélémy, Virgin Islands.[http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=6&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEIQFjAF&amp;amp;url=http%3A%2F%2Fwww.cabi.org%2FISC%2FFullTextPDF%2F2013%2F20133231104.pdf&amp;amp;ei=VeZ8U_eFNaqX1AXh_YHYDA&amp;amp;usg=AFQjCNGWK-GGi3IRhAfSNBg6nah3XhoKWw&amp;amp;bvm=bv.67229260,d.d2k]&lt;br /&gt;
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[u]Natural enemies[/u]; Natural enemies of scales include parasitic wasps, ladybird beetles and lacewings, which are killed by broad-spectrum pesticides. Because of their honeydew secretion, scales may be protected / farmed by specific ants (eg arboreal ants (Azteca instabilis) farming soft green scales).[http://www.ncbi.nlm.nih.gov/pubmed/18559179] Some fungal pathogens effect this symbiotic interaction.[http://www.ncbi.nlm.nih.gov/pubmed/23905728] Parasitoid insects use the honeydew secreted by coccids (and aphids etc) as an infochemical to locate their hosts.[http://www.ncbi.nlm.nih.gov/pubmed/15112724] R. iceryoides is parasitized by Dinocarsis sp., Microterys flavus, Metastenus concinnus and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
Of the parasitoids Acerophagus papayae and Anagyrus loecki, A. papayae is the most effective in decimating the papaya mealybug. (Meyerdirk)&lt;br /&gt;
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[u]Lacewings[/u]; Most lacewings (also) feed on plant material, such as pollen. Particularly green lacewings such as Mallada boninensis, Mallada astur and Mallada signata are mainly predatory and very effective. They are relatively small (length up to 1.5 cm, including wings). Adults are active during the night, and may be attracted by flashlight. Eggs are attached to leaves and hatch within 4 days. The larval period lasts 12 days, and the pupal period lasts about 9 days. Adults live just one month. One may commercially rear millions of green lacewings for biological control of mango pests.&lt;br /&gt;
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[u]Cryptolaemus montrouzieri[/u] (aka Mealybug Ladybird) is a (4 mm long) predatory ladybird that eats mealybugs, soft scales (Coccidae) and armoured scales (Diaspididae). Originally from Australia, introduced in Florida and commercially available as a mealybug control agent. It needs warm, humid weather. Survival temperature range is 15-35°C. Life cycle on average is 30 days under optimum conditions: 23°C and 60-70% humidity. Optimal temperature for adults is 28°C. They are active between 16°C and 33°C, and 70 to 80% RH. Longevity of females is 70 days max. It produces 4 generations per year. Development is accelerated at higher temperatures. At 25°C it may lay a total of 200 to 700 eggs (av. 400), at the rate of averagely 9 eggs per day. They preferrably lay their eggs in the egg mass of mealybugs. The eggs hatch in 5 to 6 days. There are 4 larval stages, and the entire larval period lasts about 12 to 17 days. At pupation stage they are about 13 mm long. Adults emerge after 7 to 10 days. These beetles prefer high concentrations of mealybugs to predate on, and will fly away when concentrations of mealybugs are lower.[http://www.arabscientist.org/english/page/11/] During its larval development, one C. montrouzieri may eat 2400 eggs of C. polygonata.[http://www.cabdirect.org/abstracts/19981112241.html] Unfortunately, the filaments produced by its larvae look very similar to mealybugs. Initially release 1 or 2 new active beetles per square meter in the morning, before it gets hot. Subsequently regularly release 2 to 3 beetles per tree. The beetles will be killed by pesticides, but tolerate copper-fungicides.&lt;br /&gt;
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[u]Chilocorus kuwanae[/u]; This ladybird beetle is a primary predator of wax scales (Ceroplastes sp.). This ladybird beetle is attracted by (spraying) methyl jasmonate, mainly due to the terpenoid compound alpha-pinene [http://www.ncbi.nlm.nih.gov/pubmed/19825299]&lt;br /&gt;
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[u]Microweisea coccidivora[/u]; a tiny (1mm long) predatory ladybird beetle from Florida and South Carolina (USA) that feeds particularly on armoured scales.&lt;br /&gt;
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[u]Azya orbigera[/u]; The larvae of this ladybird beetle predate on Coccus viridis. These larvae are relatively immune to ant attacks (ants that farm coccids), due to their sticky waxy filaments. Also, the presence of ants reduces A. orbigera larvae getting parasitized.[http://www.ncbi.nlm.nih.gov/pubmed/18348805] A. orbigera has been observed in Colombia, Guyana, Venezuela, Bélize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, México, USA, Trinidad and the West Indies.[http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php]&lt;br /&gt;
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[u]Sumnius cardoni[/u] (a predatory ladybird) predates on nymphs of D. mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19810586735.html] &lt;br /&gt;
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[u]Coccodiplosis sp[/u] (a parasitic gall midge)[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9], Cybocephalus sp. (a predatory beetle) and Scymnus coccivora (a predatory ladybird) predate on R. iceryoides (mealybug). &lt;br /&gt;
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[u]Cryptochetum sp.[/u] (parasitic flies) parasitize the 1st- and 2nd-instar nymphs of the Drosicha mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
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[u]Coccophagus scutellaris[/u] parasitizes Coccus hesperidum by laying eggs in its gut.[http://www.ncbi.nlm.nih.gov/pubmed/747455] When parasited and parasiteless hosts are available, the female of Coccophagus deposits more eggs on the latter.[http://www.ncbi.nlm.nih.gov/pubmed/7283545]&lt;br /&gt;
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[u]Thomsonisca pakistanensis[/u] is a parasitic wasp that most commonly parasitizes Aspidiotus destructor. A. destructor is also parasitized by Aneristus ceroplastae (tiny parasitic wasp), Comperiella bifasciata (parasitic wasp also effective against yellow scales; Aonidiella citrina[http://www.publish.csiro.au/paper/ZO9710053.htm]), Chartocerus sp. and Chrysonotomyia sp. (both also parasitic wasps) [http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
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[u]Aphytis sp[/u]; This tiny (2 to 3 mm long) parasitic wasp parasitizes Aulacaspis tubercularis (up to 46% parasitism in a test).[http://www.actahort.org/books/509/509_96.htm]&lt;br /&gt;
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[u]Lecanicillium lecanii[/u]; This fungus kills various coccids, including Coccus hesperidum.[http://www.ncbi.nlm.nih.gov/pubmed/20863711] Also Ceroplastes sp. may be killed by Lecanicillium lecanii. Adding body materials of life coccids to a multi-generation culture (medium), significantly keeps the vigor and higher virulence of this fungus.[http://www.ncbi.nlm.nih.gov/pubmed/20387464]&lt;br /&gt;
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[u]Control[/u]: Collect the affected leaves and burn them. Spraying chlorogenic acid (a phenol naturally present in coffee beans) stimulates locomotory activity of the green scale Coccus viridis, thus minimizing their feeding. [http://www.ncbi.nlm.nih.gov/pubmed/20857759]&lt;br /&gt;
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If you have no other option: Use Metasystox 25% EC at the rate of 0.3 L in 450 L water or Fotidal 50 EC at the rate of 0.5 L in 450 L water / acre. Or spray mineral oil at low concentrations (not damaging the trees) after fruit picking, but not during flowering. Don&amp;#039;t spray during droughts or excessive heat.&lt;br /&gt;
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==Weevils==&lt;br /&gt;
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[u]The Mango Stone Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_mangiferae.jpg|thumb|left| Mango Stone Weevil [http://en.wikipedia.org/wiki/Sternochetus_mangiferae#mediaviewer/File:Mango_seed_weevil_2.jpg © P.Jeganathan]]]&lt;br /&gt;
[[Image:Oecophylla_smaragdina.jpg|thumb|right| Stone Weevil captured by O. smaragdina [http://www.cabi.org/isc/datasheet/16434 © Renkang Peng]]]&lt;br /&gt;
[[Image:Mantis_religiosa.jpg|thumb|right| Praying mantis [http://www.statesymbolsusa.org/Connecticut/insect_praying_mantis.html © Andy Williams / CritterZone]]]&lt;br /&gt;
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The Stone Weevil (Sternochetus/Curculio/Rhynchaenus/Cryptorhynchus mangiferae) aka Mango Seed / Nut Weevil is a short weevil; 7 mm to 1 cm long and 4 mm wide. It possesses a tough exoskeleton. Weevils are a type of beetle. The scales of the Stone Weevil may be of various colours; shades of black, grey, red or yellow. The Stone Weevil has been observed in many mango-producing countries all over the world (including Ghana, Nigeria, Kenya, Mozambique, Tanzania, Uganda, Zambia, India, Bangladesh, Indonesia, Thailand, Malaysia, Australia, USA)[http://www.cabi.org/isc/datasheet/16434]. Adult weevils can hardly fly, and this pest spreads by fruit transports. At dusk and during the night, the adults feed on leaves and young shoots. The female lays her eggs on and just under the surface of the peel of young (about 3 cm long) mangoes. Each female can lay up to 15 eggs per day and may lay several eggs on one fruit. The Stone Weevil prefers the sweetest (high-sugar) varieties of mango. After laying an egg, the weevil will make an incision in the fruit to let the sap from the fruit cover the egg, and to make it stick to the fruit. The amber-coloured sap will harden out, and remain as a protective resin mark over the egg. Newly emerged grubs are white and slender, and have no legs. They bore their way through the pulp towards the seed, within 2 days. Larvae and pupae develop inside the fruit. Pupation occurs in the seed. No external symptoms of attack are readily visible on infested fruits. Once matured (which takes up to 2 months), they eat their way out. About 25% of adult the weevils over-winter in the seed. They can live 2 years. One generation is produced each year.&lt;br /&gt;
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Natural enemies; The Stone Weevil is effectively predated on by Weaver ants such as Oecophylla smaragdina and Oecophylla longinoda.[http://iiste.org/Journals/index.php/JBAH/article/view/12284] Weaver ants, however, may also farm (for the produced honeydew) and protect aphids and various mango scales against their natural enemies. &lt;br /&gt;
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Weevils are also predated on by [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids], which may eat anything they can catch, including flies, beetles and moths. Adults readily mate in captivity and have one generation per season. Rearing mantids requires rearing of other insects, such as aphids and drosophilae (for nymphs), tephritids (for young mantids) and moths (for adult mantids) in large quantities. Females lay their eggs in a sticky cluster of several cm long. Nymphs (very tiny praying mantids) emerge from eggs. The developing nymphs need to be separated and fed aphids/drosophilae, or they will become cannibalistic.&lt;br /&gt;
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Control; Collect and destroy all fallen fruits, seeds, leaves, twigs etc. If you have no other option, spraying Fenthion (0.01%) on the trees at the time of oviposition is found to be effective. Fenthion, however, is extremely toxic to beneficial insects [http://www.actahort.org/books/509/509_96.htm], so that applying Fenthion may lead to secondary infestation of Mealybugs. A single spray of tiametoksam (Actara™ SC 240g/ℓ a.i.) at the roots during flower set may be effective for seasonal control.[http://etd.uovs.ac.za/ETD-db/theses/available/etd-09182009-083002/unrestricted/LouwCE.pdf] In Alphonso and Bagan Pali mangoes, a single spray (at dusk) of Monocrotophos 36EC 1ml per 1 litre of water was found to be very effective (97.5% to 100% mortality) [http://issuu.com/kisanadmin/docs/mango1/16] In a laboratory test, Beauveria bassiana was somewhat effective (30% mortality in 14 days), but in an orchard setting there was no effect at all.[http://www.cabdirect.org/abstracts/19971101160.html]&lt;br /&gt;
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[u]The Mango Pulp Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_frigidus.jpg|thumb|left| Mango Pulp Weevil [http://www.thaibugs.com/?page_id=284 © Thaibugs]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
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The Mango Pulp Weevil (Sternochetus frigidus or Cryptorrhynchus gravis) has been observed in Bangladesh, India, Indonesia, Malaysia, Myanmar,&lt;br /&gt;
Pakistan, Papua New Guinea, Philippines, Singapore and Thailand. This female weevil lays eggs (25-60 eggs / week) on mango fruits (minimum 6 cm diameter). Newly hatched larvae tunnel into the fruit pulp. The larvae form a chamber (constructed of frass) next to the seed, from which they eat their way through the pulp. Pupation occurs within these chambers. Matured weevils leave the ripe fruit through an exit hole in the peel. Prior to this exit, nothing shows that the fruit is infected. They are fully matured after 6 weeks; able to mate, repeatedly. One complete life cycle varies from 34-35 days. More than half of adult weevils hibernate in seeds. Mango Pulp Weevils are very poor flyers (maximum 90 cm horizontally).[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Thrips==&lt;br /&gt;
[[Image:Frankliniella_occidentalis.jpg|thumb|left| F. occidentalis [http://conabio.inaturalist.org/taxa/243761-Frankliniella © Th0th]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Thrips, or thunderflies, thunderbugs, storm flies, thunderblights, storm bugs, corn flies or corn lice may feed on many plants. Frankliniella bispinosa, Frankliniella kelliae (Florida, Caribbeans) and Frankliniella occidentalis (California, Israel) also feed on mango. They feed on young leaves, shoots and flowers, causing discoloration and deformation.&lt;br /&gt;
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Thrips are tiny (1 mm long on average; varying from 0.5 mm to 1.4 cm). They are not good flyers, but may readily be carried by the wind. Given the right conditions, they may rapidly multiply and form large swarms. To inspect trees for thrips infestation, just hold a bucket and shake a branch, and some thrips will fall off the infested branch into the bucket.&lt;br /&gt;
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Control: Natural enemies of thrips are Green lacewings (eg Mallada boninensis, Mallada signata), Orius insidiosus, Anystis agilis and Hypoaspis aculifer. Biological insecticides such as Beauveria bassiana and Verticillium lecanii may be effective.&lt;br /&gt;
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[u]Orius insidiosus[/u] is a 3 mm long pirate bug (aka insidious flower bug). They feed on thrips, mites, small caterpillars and various insect (including aphids&amp;#039;) eggs and larvae, and also pollen. They prefer eating thrips over aphids.[http://www.ncbi.nlm.nih.gov/pubmed/18845007] Orius may kill several thrips per day, beyond their need for nutrients. Adults fly well, and may readily disperse, looking for prey. O. insidiosus may be mass reared on [http://www.waiwiki.org/index.php?title=Armyworm armyworms]. They may occasionally sting people, which may hurt, but is completely harmless. Eggs are laid and embedded in the plant tissue of the fruit, stem, leave stalks and big veins at the underside of leaves. From eggs to adults, Orius goes through 5 larval stages. This takes about 16 to 18 days at 25°C. They are predatory at each stage (except as pupae). Adults live 3 to 4 weeks. As Orius albidipennis lays more eggs and has a shorter lifecycle, it may be a better biocontrol agent than Orius insidiosus.[http://eurekamag.com/research/030/621/030621437.php] Release at 2 to 3 Orius per square meter.&lt;br /&gt;
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==Whiteflies==&lt;br /&gt;
[[Image:Trialeurodes_vaporariorum.jpg|thumb|left| Trialeurodes vaporariorum [http://en.wikipedia.org/wiki/File:Weisse-Fliege.jpg © Gaucho]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| Orius insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Paraleyrodes_bondar.jpg|thumb|left| Paraleyrodes bondar [http://manateeornprod.wordpress.com/2012/02/22/new-whitefly-in-florida/ © UF/IFAS]]]&lt;br /&gt;
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Whiteflies (incl. Aleurodicus rugioperculatus, Aleurodicus dispersus, Aleurothrixus floccosus, Aleyrodes proletella, Bemisia tabaci, Paraleyrodes bondar, Siphoninus phillyreae, Trialeurodes vaporariorum) attack various crops, including mango, orange, tomato and watermelon. &lt;br /&gt;
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Whiteflies suck the saps from leaves, causing direct damage. They are also vectors of various diseases, including viruses. Additionally they may produce a lot of honeydew, which may lead to sooty mould. The density of the whitefly is positively correlated with maximum temperature and negatively correlated with relative humidity.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Natural enemies[/u]; Various animals predate on whiteflies, including green lacewings, Orius insidiosus and ladybird beetles. The minute parasitic wasps Encarsia guadeloupae and Encarsia haitiensi very effectively cause reduction in the population of Aleurodicus despersus.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Control[/u]&lt;br /&gt;
Dissolve 1 kg of sugar per 1 L of water. Poor in plastic transparent bottle. Drill holes in the upper part (above water level); small enough for whiteflies to enter, but not for bees. Put the lid on. Hang 2 to 3 bottles in each tree at flower set. Renew weekly.&lt;br /&gt;
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=Integrated Pest Management=&lt;br /&gt;
Integrated Pest Management (IPM) integrates proper orchard managment (such as sufficient spacing of trees, pruning off overlapping branches, the use of bait traps and removing affected fruits and plant parts) and the use of a variety of anti-bugs (natural enemies of mango bugs), so that they are complementary and sufficiently reduce all pest populations, minimizing the use of pesticides.&lt;br /&gt;
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Below is an example of the complementary use of anti-bugs, for both predation and parasitism.&lt;br /&gt;
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Preliminary requirements: A sealed, ventilated room. Constant supply of mangoes, or grapes, kiwi, lychee, pineapple, peach, apricot, papaya, apple or pear, to feed and rear Drosophilae (2-4mm long &amp;#039;fruit flies&amp;#039;), Tephritids (4-7 mm long &amp;#039;true&amp;#039; fruit flies) and Eudocima sp. (fruit piercing moths; up to 10 cm wingspan). One section needs daylight exposure, for growing Fabacae / Menispermacae for the Eudocima sp. to lay their eggs, and for their larvae to feed on the leaves. This sealed room should rather be too large than too small to prevent a lack of food for the reared predators. Any surplus of eggs and/or larvae may be sold as fish feed.&lt;br /&gt;
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* Rearing [http://www.waiwiki.org/index.php?title=Praying_mantis Praying Mantids] to predate on moths, caterpillars, weevils and young Longicorn beetles by adults, and on fruit flies, hoppers and midgets by young mantids. These mantids may be reared on fruit flies  (Drosophila and Tephretids) and specific moths. Young mantids should be separated after hatching, in boxes. Nymphs should be fed Drosophilae. Young mantids should be fed Tephretids. Adult mantids are fed Eudocima moths or Armyworm moths.&lt;br /&gt;
* Rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings] (Mallada signata, Mallada boninensis) to predate on aphids, scales, hoppers and thrips in the field. These lacewings may be reared on Eudocima sp. eggs collected from the Fabacae / Menispermacae in the sealed room, or on Armyworm eggs. &lt;br /&gt;
* Rearing the parasitic wasps Fopius arisanus and/or Diachasmimorpha longicaudata to parasitize Tephritids (fruit flies). Tephritid eggs should be collected from fruits in the sealed room. To prevent the fruit fly eggs from drying out, the eggs should be kept on a moist substrate. To prevent molding, a constant air current should be provided during the time required for parasitoid development.&lt;br /&gt;
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The second phase may be more labour intensive. To rear Tetrastichus sp. and Euplectrus sp. or Trichogramma sp. for the purpose of parasitizing scales, moths, midges and longicorn beetles, one most collect eggs from all species in the field. Instead, one may also rear subject pests.&lt;br /&gt;
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As an alternative for green lacewings, one may use the combination of Cryptolaemus montrouzieri (mass reared on Plancoccus citri on potatoes or squash [http://www.arabscientist.org/english/page/11/], for predating on scales) and Orius insidiosus (mass reared on [http://www.waiwiki.org/index.php?title=Armyworm Armyworms] (which are best reared on Bermudagrass), for predating on aphids, thrips and small caterpillars). This allows for a more targeted approach (if there is only 1 pest, eg scales). This may also be a better combination (together with Praying mantids) because adult Praying mantids prefer to predate on lacewings (15 mm long) over the much smaller C. montrouzieri (4 mm) and O. orius (3 mm).&lt;br /&gt;
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=Diseases=&lt;br /&gt;
Bugs cause damage to the mango tree, which makes the tree more susceptible to disease. Many bugs are also disease vectors; they transport the viruses, fungi etc. to the mango tree. Successfully combatting mango bugs, is therefore key to prevention and control of mango diseases. Using chemicals to fight mango diseases and/or bugs, however, may actually result in killing natural enemies of the bugs that spread disease.&lt;br /&gt;
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==Anthracnose==&lt;br /&gt;
[[Image:Anthracnose.jpg|thumb|left| Anthracnose [http://www.indiancropdiseases.com/mango.aspx]]]&lt;br /&gt;
[[Image:Anthracnose-2.jpg|thumb|right| Anthracnose [http://hawaiiplantdisease.net/cpg/displayimage.php?pid=397 © Nelson]]]&lt;br /&gt;
Anthracnose is caused by the fungi Colletotrichum acutatum [http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2003.93.5.579] and Glomerella cingulata [http://www.idosi.org/wjas/wjas4(6)/8.pdf][http://www.jofamericanscience.org/journals/am-sci/am0608/46_3350am0608_361_367.pdf]. It particularly affects young shoots, flowers and fruits. The disease is stimulated by high humidity, frequent rains or mists, and a temperature range of 24 to 32°C. The results of this disease are black spots on panicles, leaf spot, blossom blight, withered tip, twig blight and fruit rot. Particulary young and tender shoots and foliage are affected, causing die back of young branches. Wounded twigs may also be infected and may sometimes die off. Fruits develop black spots and rotting and young fruits may shrivel and prematurely drop. If the entire inflorescence is destroyed, no fruits will set. &lt;br /&gt;
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[u]Where?[/u] It widely occurs in the orchard and in storage, Anthracnose has been reported in Argentina, Florida, Hawaii, India, Pakistan, Philippines, Sri Lanka, South Africa and Trinidad.&lt;br /&gt;
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[u]Control[/u]: Burn all fallen leaves. Prune affected twigs and burn them. Apply Bordeaux paste on cut ends. To prevent blossom infection, spray twice at 15 days interval during flowering with Carbendazirn (Bavistin 0.1%). To prevent foliar infection, spray copper fungicides (0.3%) twice a week. Apply a teaspoon of dish detergent per spray load to make the copper sulfate stick. Prevent the copper sulfate from dripping on the ground to prevent contamination of the soil and the roots of the tree.&lt;br /&gt;
Picked mangoes should be submerged for 15 minutes in hot water (52°C) with Carbendazim (0.1%). One may also dip the mangoes in 500 ppm Benomyl and 900 ppm Thiobendazole. C. acutatum is also effectively inhibited by the synthetic strigolactone GR24.[http://www.ncbi.nlm.nih.gov/pubmed/21688170]&lt;br /&gt;
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==Bacterial Canker==&lt;br /&gt;
Bacterial black spot (bacterial canker) is a bacterial disease caused by Xanthomonas campestris pv. mangiferaeindicae. It affects many varieties. Initially, irregular small water soaked lesions will appear on the leaves. These will extend into patches of dead tissue. The leaves will turn yellow and die. The lesions will also appear on the the stalk of the leaves, twigs and fruits. The fruits will then turn brown and black, and burst open. The released fluid is filled with bacteria that will contaminate the rest of the tree or other fruits in storage.&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Immediately spray Agrimycin-100 (0.01%) or Streptocycline (0.01%), 3 times at 10 days interval. Subsequently spray Copper Oxychloride (0.3%) or Carbendazim (Bavistin 0.1%) once a month.&lt;br /&gt;
&lt;br /&gt;
==Die Back==&lt;br /&gt;
Die back (Botryodiplodia (Lasiodiplodia) theobromae aka Physalospora disrupta/quercuum/rhodina/glandicola) may occur any time of the year. It is characterized by advancing discoloration and darkening of the bark. It extends along the veins of the leaves, causing browning and upwards rolling of the margins. Eventually the leaves shrivel and fall. Twigs and branches dry and defoliate, and may drain a yellow-brown gummy fluid. It may look as if the tree has been exposed to a bush fire.&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Prune diseased twigs 10 cm below the affected area. Paste the cut ends with Copper Oxychloride (0.3%) and also spray Copper Oxychloride (0.3%) on affected trees.&lt;br /&gt;
&lt;br /&gt;
==Diplodia Stem-end Rot==&lt;br /&gt;
This fungus (Lasiodiplodia theobromae) may enter the mango where the skin or stem has been injured mechanically. It will form a black circle around the pedicel.&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Prevent mechanical injuries. After picking, submerge mangoes for 15 minutes in hot water (53°C) with Carbendazirn (0.1%).&lt;br /&gt;
&lt;br /&gt;
==Mango Black Blight==&lt;br /&gt;
Black blight is caused by a fungus (Capnodium mangiferum).&lt;br /&gt;
&lt;br /&gt;
==Mango Blight==&lt;br /&gt;
Mango blight is caused by Erwinia bacteria (Enterobacteriaceae). Many spots appear on the leaves which cause a reduction in growth and yield. &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Use Dithane M 45 at the rate of 1.7g/L (450 L water per acre).&lt;br /&gt;
&lt;br /&gt;
==Mango Malformation==&lt;br /&gt;
Mango malformation (Fusarium mangiferae) has been reported in Australia, Bangladesh, Brazil, Central America, Cuba, Egypt, India, Israel, Malaysia, Mexico, Pakistan, South Africa, Sudan, Switzerland, UAE and the USA. It is a disease caused by fusarium species [http://www.mycologia.org/content/94/4/722.full][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.6.456] (F. subglutinans from Egypt, Florida, Israel, Malaysia and South Africa, F. sterilihyphosum from Brazil and South Africa, and Fusarium sp. nov. and F. proliferatum from Malaysia)[http://www.ncbi.nlm.nih.gov/pubmed/18943188][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-96-0667 Free Full Text]. The disease is stimulated by relatively cool conditions; 10 to 27°C, and merely survive hotter conditions. In this disease the leaves and inflorescence of the mango tree are badly deformed and gradually dry up. There is no fruit setting and hence no production. &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Various fungicides, including Captan, Benomyl and Thiram have been reported to be effective to some extent. &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Mango Scabs==&lt;br /&gt;
&lt;br /&gt;
Mango Scabs (Denticularia mangiferae aka Sphaceloma mangiferae aka Elsinoë mangiferae) survives on young tissues (young leaves, shoots, flower, fruits) of the mango tree only. Adult tissue is not susceptible. Its spores (and their germination) requires rain splash and free water to produce new infections.[http://www.cerambycoidea.com/titles/affa2004.pdf] Moisture conditions stimulate its growth. Though this parasite is not a fungus but in a persistent parasitic relationship with the mango tree, it produces symptoms that look somewhat familiar to those of anthracnose (a fungus) infections. It starts with small light- or dark brown or grey spots on the underside of leaves and fruit. These spots get bigger and darker with time. In the center of this lesion a cracked texture will appear. If the host tissue survives, lesions may develop into scar tissue. When infestation is severe, there is loss of leaves and fruit.&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]; Remove and destroy all fallen leaves, twigs, branches etc. Spray copper hydroxide or copper oxychloride on a very regular basis, particularly in the rainy season. Without chemical control, losses as high as 90% have been observed.[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
&lt;br /&gt;
==Phoma Blight==&lt;br /&gt;
Phoma blight (Phoma glomerata) is a fungus that affects the fibre in the top layer of old leaves. It produces small, irregular, angular lesions. As the lesions grown, the colour changes from yellow-brown to cinnamon. Many small spots may form overwhelming patches and result defoliation of affected leaves. Phoma glomerate very effectively produces an enzyme that converts the remainders (glucose) of fibre (from the leaves) into it the main structural component (N-acetyl-D-glucosamine)[http://www.ncbi.nlm.nih.gov/pubmed/15553782] in its cell walls, thus enabling its own growth.&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Immediately spray Benomyl (0.2%), and 20 days later spray 0.3% Miltox (Copper Oxychloride plus Zineb). Quadris (azoxystrobin) combined with thymol at a non-fungitoxic concentration produces much higher growth inhibition of Phoma glomerata than the fungicide alone.[http://www.ncbi.nlm.nih.gov/pubmed/22408641]&lt;br /&gt;
&lt;br /&gt;
==Powdery Mildew==&lt;br /&gt;
Powdery mildew (Oidium mangiferae) affects virtually all mango varieties. Characteristically, a white superficial powdery fungal grows on the inflorescence and tender leaves, stalk of panicles, flowers and young fruits. The affected flowers and fruits will pre-maturely drop, or fruit setting does not occur at all. The disease is initiated and developed by warm (&amp;gt;17°C) dry weather, but during the flowering stimulated by prolonged rains or mists and by cool nights. The disease has been reported in Brazil, India, Jamaica, Pakistan, Sri Lanka, South Africa and the U.S.A. &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Alternately spray wettable sulphur (0.2% ; 2 g Sulfex/L), Tridemorph (O.1% ; 1 ml Calixin/L) and Bavistin (0.1%) at 15 days interval. Start spraying when the panicle (flower cluster) starts to emerge. Or spray with Carbendazim (0.1%) or Tridemefon (0.05%) or wettable sulphur (0.3%) alone (3 days in a row).&lt;br /&gt;
&lt;br /&gt;
[u]Control-2[/u]: Similar to A. quisqualis AQ10 Biofungicide in commercial use for biocontrol of powdery mildew, Phoma glomerata (see [http://www.waiwiki.org/index.php?title=Mango#Phoma_Blight Phoma Blight] above) may act as mycoparasite of powdery mildew. Phoma glomerata can colonize and suppress development of powdery mildew.[http://www.ncbi.nlm.nih.gov/pubmed/10618259] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91841/ Full free text]&lt;br /&gt;
&lt;br /&gt;
==Red Rust==&lt;br /&gt;
Red rust (Cephaleuros virescens aka C. parasiticus) initially causes round and slightly elevated green-grey spots mainly on leaves. They turn into rusty red spots and may form bigger patches. This reduces the photosynthetic capacity of the leaves. In severely infected trees, twigs will remain stunted, the bark and twigs will get thick and the leaves will dry up and drop off. &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Spray Copper Oxychloride (0.3%) 3 times.&lt;br /&gt;
&lt;br /&gt;
==Sooty Mould==&lt;br /&gt;
Sooty mould (Meliola mangiferae) may be the result of too many insects in your orchard. Scale insects, mealy bug and hoppers secrete honey dew. The honey dew sticks to the leaves, feeding fungal growth. This results in a black sooty mould over the entire surface of leaves and twigs, affecting the photosynthetic capacity of the leaves. Uncontrolled, the tree may completely turn black.&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Prune and burn the affected branches. Spray Wettasulf (0.2%) with+ Metacid (0.1 %) and gum acacia (0.3%). Ants feed on the honeydew excreted by soft scales, preventing accumulation of sooty moulds, but they also protect the scales from natural enemies.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Silica_in_Poaceae&amp;diff=4428</id>
		<title>Silica in Poaceae</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Silica_in_Poaceae&amp;diff=4428"/>
		<updated>2014-12-24T16:48:10Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Silicon for algae==&lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Tilapia Tilapia] naturally thrive on diatoms and Spirulina, which are [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae].&lt;br /&gt;
Diatoms are the world’s largest contributors to biosilicification.[http://www.academia.edu/8565023/SILICON_METABOLISM_IN_DIATOMS_IMPLICATIONS_FOR_GROWTH_1] Most diatom species have an obligate requirement for silicon for cell wall formation.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259041/] Particularly diatoms that thrive in saline, alkaline conditions generally require high silicon:phosphorus ratios, including Anomoeoneis sphaerophora, Rhopalodia species, Melosira granulata [http://www.tandfonline.com/doi/abs/10.1080/0269249X.2003.9705580#.VIgW0zGG811], Nitzschia species [http://tube.aslo.net/lo/toc/vol_18/issue_1/0053.pdf] and Synedra ulna [http://www.aslo.org/lo/toc/vol_31/issue_6/1169.pdf]. Compost may be used for supplying nutrients for the cultivation of Spirulina and diatoms. Compost may be derived from composting toilets. Such [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets] frequently need the addition of dried plant material (sawdust, dried grasses or -leaves) as a carbon source. Silicon is the second most abundant element in soil. To add silicon along with the carbon, the used plant material should be silicon accumulating.&lt;br /&gt;
&lt;br /&gt;
==Silica in plants==&lt;br /&gt;
Plants may take up silicon from the soil if the pH is below 9. Biogenic silica is known to alleviate plant stressors such as high temperatures and drought.[https://www.jstage.jst.go.jp/article/pps1998/1/2/1_2_96/_article] There may be no relationship between soil type and plant Si concentrations, but with temperature and precipitation.[http://www.sciencedirect.com/science/article/pii/S0961953411001516] Water use per biomass is lowest in regions with a high rainfall to evaporation ratio. Silicification in non-grass species is probably less dependent on soil silica availability [http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0706.2012.20057.x/abstract;jsessionid=70C8056AF4CF43FA866C262AAF3D6DC0.f02t03] and water availability.[http://www.sciencedirect.com/science/article/pii/S0925857405001412].&lt;br /&gt;
&lt;br /&gt;
==Grasses==&lt;br /&gt;
Leaf silica concentration in tall grass species increases with watering [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204439/] and with age.[http://www.abz.org.br/files.php?file=documentos/R1172_1_630921223.pdf] Blade silica content is higher after the grass has been defoliated.[http://www.pnas.org/content/80/3/790.short] Silica levels are lowest in the stem fraction. The level of silica in grasses may co-depend on the level of silica in the soil, availability of water [http://www.sciencedirect.com/science/article/pii/S0367253013000777], the pH of the soil and temperature. Grasses adapted to hot climates and atmospheres low in carbon dioxide (C4 grasses), usually have lower silica contents than C3 grasses (cool-season grasses). There is no consistent relationship between silica content and annual precipitation.[http://pubs.aic.ca/doi/pdf/10.4141/cjps67-009] Grasses may also solubilize silicate from clay. [http://www.pnas.org/content/80/3/790.full.pdf] In perennial grasses, the main source of silica is from monosilicic acid (soluble silica) in the water. Silica levels in grasses are highly influenced by the monosilicic acid contents of the soil. As a result, clay soils result in higher silica contents in grasses than sand.[http://www.reap-canada.com/online_library/feedstock_biomass/24%20Strategies%20to.pdf]&lt;br /&gt;
&lt;br /&gt;
Wheat (&amp;#039;&amp;#039;Triticum spp.&amp;#039;&amp;#039;) are grass species and may contain 0.3 to 10% silicon.[http://books.google.nl/books?id=or98NnK10iYC&amp;amp;pg=PA277&amp;amp;dq=Avena+sativa+Panicum+texanum+Lolium+perenne+silica&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=XyZ2VJGJKseS7AaRroGoAw&amp;amp;ved=0CCsQ6AEwAA#v=onepage&amp;amp;q=Avena%20sativa%20Panicum%20texanum%20Lolium%20perenne%20silica&amp;amp;f=false] &amp;#039;&amp;#039;Triticum aestivum&amp;#039;&amp;#039; may contain 2.5%, &amp;#039;&amp;#039;Triticum boeoticum&amp;#039;&amp;#039; 2.5%, &amp;#039;&amp;#039;Triticum dicoccoides&amp;#039;&amp;#039; 1.2% and &amp;#039;&amp;#039;Triticum percicumx&amp;#039;&amp;#039; 1.6% silica. Rice (&amp;#039;&amp;#039;Oryza sativa&amp;#039;&amp;#039;) may contain 4.2% silica. [http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html] &amp;#039;&amp;#039;Pennisetum purpureum&amp;#039;&amp;#039; (Elephant grass, a Panacea) may contain 0.85% silica [http://www.biomedcentral.com/content/pdf/1754-6834-7-10.pdf] Particularly &amp;#039;Millet-Paniceae&amp;#039; may be effective silica accumulators, such as some Brachiaria and Panicum species.&lt;br /&gt;
&lt;br /&gt;
==Brachiaria==&lt;br /&gt;
&lt;br /&gt;
[[Image:Brachiaria_decumbens.jpg|thumb|left| Brachiaria decumbens [http://www.agrolink.com.br/agricultura/problemas/busca/capim-braquiaria_103.html]]]&lt;br /&gt;
[[Image:Brachiaria_decumbens_2.jpg|thumb|left| Brachiaria decumbens [http://www.marangatu.com.br/produtos/ybiete/brachiaria-decumbens/]]]&lt;br /&gt;
&lt;br /&gt;
Brachiaria species (&amp;#039;Signal grass&amp;#039;) are C&amp;lt;sub&amp;gt;4&amp;lt;/sub&amp;gt; (&amp;#039;Millet&amp;#039;) grasses that grow fast.[http://link.springer.com/article/10.1007%2Fs11104-010-0472-5#page-2] &amp;#039;&amp;#039;Brachiaria brizantha&amp;#039;&amp;#039; may contain 1.38% silicon [http://www.biomedcentral.com/content/pdf/1754-6834-7-10.pdf] Brachiaria brizantha adapts poorly to poorly drained soils.[http://www.tropicalforages.info/key/Forages/Media/Html/Brachiaria_brizantha.htm] &lt;br /&gt;
&lt;br /&gt;
Particularly &amp;#039;&amp;#039;Brachiaria decumbens&amp;#039;&amp;#039; (aka Urochloa decumbens, aka Surinam grass / Signal grass) may be considered silicon accumulating (3.6% in adult leaves[http://www.abz.org.br/files.php?file=documentos/R1172_1_630921223.pdf]) and resistant to droughts.[http://www.scielo.br/scielo.php?pid=S0103-90162003000400022&amp;amp;script=sci_arttext] It occurs in Burundi, Rwanda, Democratic Republic of Congo, Kenia, Tanzania and Uganda.[http://grassworld.myspecies.info/sites/grassworld.myspecies.info/files/UV_2.pdf] Silica levels in Brachiaria decumbens are highest in mature leaves, particularly on the upper epidermis of the grass leaf blades.[http://connection.ebscohost.com/c/articles/54568182/silicon-distribution-accumulation-shoot-tissue-tropical-forage-grass-brachiaria-brizantha] &lt;br /&gt;
Yields of 10 tonnes (rainfed) and 19.5 tonnes (irrigated) dry matter/ha/year have been reported.[http://www.cabdirect.org/abstracts/19826744618.html;jsessionid=D942564286C537330F0EE8A0C86F1BDC] This may in part be due to its capacity of evapotranspirative adaptation in relation to the dynamic between water-transpirating and water absorbing surfaces as well as surface conductance sensitivity to soil transfer and yielding capacity.[http://www.sciencedirect.com/science/article/pii/S0168192397000920] &lt;br /&gt;
In phosphorus-deficient conditions, Brachiaria decumbens very effectively increases phytase and phosphatase activity to utilize inositol hexaphosphate in soil.[http://link.springer.com/article/10.1023/A:1004264002524#page-1]&lt;br /&gt;
Brachiaria decumbens may grow in low fertility, low pH (as low as pH 3.5) soil, while it does not respond to lime, but responds strongly to N and P fertiliser.&lt;br /&gt;
It cannot persist on heavy clays subject to waterlogging, as its root system is very fine with low tolerance of poor drainage. Brachiaria decumbens stays green well into the dry season. It is most productive on open grasslands in the lowland humid tropics with rainfall &amp;gt;1,500 mm annually and a dry season of up to 5 months. It can tolerate short-term flooding and prefers temperatures above 19ºC. It is very tolerant of heavy grazing but less tolerant under reduced light than in full sun.[http://www.tropicalforages.info/key/Forages/Media/Html/Brachiaria_decumbens.htm]&lt;br /&gt;
Grasses decompose relatively slowly. The rate of decomposition of organic matter from Brachiaria decumbens is considered intermediate. Remaining polyphenols may serve as an indicator of litter decomposition.[http://www.sciencedirect.com/science/article/pii/003807179390050L] Chopping reduced contents of acetic acid, propionic acid and butyric acid and increases NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;-N content.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-CYXB200901008.htm]&lt;br /&gt;
&lt;br /&gt;
Brachiaria decumbens spreads by seed dispersal. It is an apomictic tetraploid that reproduces mainly by facultative apospory.[http://link.springer.com/article/10.1007/BF00021394#page-1] Reproduction approximates a diploid-tetraploid-(di)haploid reproductive cycle that does not involve triploids. Natural Brachiaria decumbens tetraploid populations are widespread, whereas diploid populations are rare. Tetraploids are considered obligate aposporous apomicts, propagating clonally, with the absence of genetic variation.[http://link.springer.com/article/10.1007/s004970050170#page-1] Even though Brachiaria decumbens is considered obligate apomitic, completely sexual accession does occur. The sexual accessions are diploids (2n = 2x = IS).[http://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_37-38/43675.pdf]&lt;br /&gt;
Brachiaria decumbens may use allelopathy to outcompete other grasses. Germinating seeds and the aqueous leachates of Brachiaria decumbens reduce the germination of other grass species, such as Phalaris canariensis, Lactuca sativa and Melinis minutiflora.[http://www.scielo.br/scielo.php?pid=S1516-89132008000400021&amp;amp;script=sci_arttext] Brachiaria decumbens has abnormal nucleolar cycle behavior.[https://www.jstage.jst.go.jp/article/cytologia/67/4/67_4_355/_article]&lt;br /&gt;
&lt;br /&gt;
Brachiaria decumbens contains several saponins and sapogenins [http://www.scielo.br/scielo.php?pid=S0103-50532002000200002&amp;amp;script=sci_arttext&amp;amp;tlng=pt], including 3-spirostanols [http://europepmc.org/abstract/med/1509678], mainly diosgenin, and smilagenin, sarsasapogenin [http://europepmc.org/abstract/MED/10839316], epismilagenin and episarsasapogenin.[http://www.sciencedirect.com/science/article/pii/0039128X9390043M] Sapogenins are saponins that may contain a steroid frameworks as their key organic feature.[http://en.wikipedia.org/wiki/Sapogenin] In tilapia, saponins may increase growth and LH release [http://www.ncbi.nlm.nih.gov/pubmed/12458187], inhibit intestinal magnesium uptake [http://www.ncbi.nlm.nih.gov/pubmed/8952951] and diminish egg production by females.[http://www.ncbi.nlm.nih.gov/pubmed/11423383] In sheep, the saponins are metabolized in the rumen, and absorbed sapogenins are excreted in the bile as calcium salts of the sapogenin glucuronides.[http://link.springer.com/chapter/10.1007/978-1-4613-0413-5_32#page-1] Saponins may be degraded by aerobic microorganisms.[http://datateca.unad.edu.co/contenidos/203015/Referencias_para_lecciones_evaluativas/Unidad_4/Ecosistema_ruminal.pdf] [http://aem.asm.org/content/7/5/304.full.pdf] Composting involves degradation by aerobic microorganisms. So that decomposted Brachiaria decumbens leaves may be a safe source of silica for diatoms in water that also ends up in tilapia ponds.&lt;br /&gt;
&lt;br /&gt;
==Panicum==&lt;br /&gt;
&lt;br /&gt;
[[Image:Panicum_texanum.jpg|thumb|left| Panicum texanum [http://twig.tamu.edu/keyindex.htm]]]&lt;br /&gt;
[[Image:Panicum_texanum_3.jpg|thumb|right| Panicum texanum [https://gobotany.newenglandwild.org/species/urochloa/texana/]]]&lt;br /&gt;
[[Image:Panicum_texanum_2.jpg|thumb|left| Panicum texanum [http://twig.tamu.edu/keyindex.htm]]]&lt;br /&gt;
&lt;br /&gt;
Panicum species (&amp;#039;Panic grass&amp;#039;) are large tropical (&amp;#039;millet&amp;#039;) grasses. &amp;#039;&amp;#039;Panicum maximum&amp;#039;&amp;#039; (&amp;#039;Buffalo grass&amp;#039;) may contain 1.07% [http://www.biomedcentral.com/content/pdf/1754-6834-7-10.pdf] or 0.5% silica.[http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html]&lt;br /&gt;
&amp;#039;&amp;#039;Panicum virgatum&amp;#039;&amp;#039; (&amp;#039;Switch grass&amp;#039;) may contain 1.5% [http://www.sciencedirect.com/science/article/pii/S0961953411001516] or 2.0% silica.&lt;br /&gt;
&amp;#039;&amp;#039;Panicum commutatum&amp;#039;&amp;#039; may contain 4.2% and &amp;#039;&amp;#039;Panicum texanum&amp;#039;&amp;#039; may contain 8.0% silica [http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html] or 7%.[http://books.google.nl/books?id=or98NnK10iYC&amp;amp;pg=PA277&amp;amp;dq=Avena+sativa+Panicum+texanum+Lolium+perenne+silica&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=XyZ2VJGJKseS7AaRroGoAw&amp;amp;ved=0CCsQ6AEwAA#v=onepage&amp;amp;q=Avena%20sativa%20Panicum%20texanum%20Lolium%20perenne%20silica&amp;amp;f=false] &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;Panicum texanum&amp;#039;&amp;#039; (aka Brachiaria texana or Urochloa texana, aka Buffalo grass, Colorado grass, Texas signal grass or Texas millet) has up 2 cm wide leaves (7.5-28 cm long) that are covered with soft minute hairs on both surfaces. Its stems may be up to 136 cm long, and the grass may grow 80 cm high.[http://oak.ppws.vt.edu/scott/weed_id/pante.htm] It is a common troublesome weed in many commercial crops, including field corn (maize)[http://www.wssajournals.org/doi/abs/10.1614/WT-05-145.1?journalCode=wete] and peanut.[http://www.peanutscience.com/doi/abs/10.3146/0095-3679(2005)32%5B68:TPPTII%5D2.0.CO%3B2] It grows best at higher temperatures. It may grow well at 30°C during the day and 24°C during the night. Its low temperature threshold for growth is ~11°C. Panicum texanum attains 25% of its maximum growth at an average daily temperature of 21°C, 50% at 24°C and 75% at 26°C. Partitioning of biomass into stems increases with higher nocturnal temperatures.[http://www.jstor.org/discover/10.2307/4044888?sid=21105506200593&amp;amp;uid=3738736&amp;amp;uid=4&amp;amp;uid=2]&lt;br /&gt;
Panicum texanum may produce 53,000 [http://www.buffelgrassseed.com/ seeds] per plant.[http://www.jstor.org/discover/10.2307/4044490?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21105315757003] (and therefore used by bird-enthusiasts[http://www.pogueagri.com/Texas_Panicum.aspx]) It grows along streams, in open fields, on prairies and on deep moist soils, southern USA and Mexico. Both annual and perennial. It is used for hay.[http://books.google.nl/books?id=8ieqQs7hIREC&amp;amp;pg=PA2310&amp;amp;lpg=PA2310&amp;amp;dq=Panicum+texanum+Africa&amp;amp;source=bl&amp;amp;ots=q15caM-8Ki&amp;amp;sig=CxZPDRCEW9lFse1cHF1gi_aWwJE&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=ZaV4VJnhFozEPJPBgbAF&amp;amp;ved=0CCEQ6AEwAA#v=onepage&amp;amp;q=Panicum%20texanum%20Africa&amp;amp;f=false] It is best adapted to well-drained sandy soils, and usually produces 23,000 seeds per plant.[http://www.jstor.org/discover/10.2307/4044889?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21105316671323] Vegetative stage Panicum texanum may contain 16% crude protein. It may contain 11% crude protein at flower/boot stage and 8% crude protein at fruit/head stage. Subsequent invitro dry matter digestibility may range from 74 to 52%.[http://www.pubs.ext.vt.edu/418/418-150/418-150_pdf.pdf] Panicum texanum occurs in Queensland and New South Wales (Australia), Arizona, California. New Mexico, Texas. Alabama, Arkansas, Florida, Georgia, Louisiana and South Carolina (USA) and North-east Zacatecas (Mexico).[http://grassworld.myspecies.info/sites/grassworld.myspecies.info/files/UV_2.pdf] Panicum texanum is preferentially consumed by quails.[http://www.jstor.org/discover/10.2307/3795575?sid=21105506200593&amp;amp;uid=3738736&amp;amp;uid=4&amp;amp;uid=2]&lt;br /&gt;
&lt;br /&gt;
==Other Poaceae==&lt;br /&gt;
Wetland Gramineae may accumulate up to 15% dry weight silica.[http://www.ncbi.nlm.nih.gov/pubmed/15012222/] Besides grasses, &amp;#039;&amp;#039;Pleioblastus chino&amp;#039;&amp;#039; 5.1% (a bamboo species) may contain 5.1% silica, &amp;#039;&amp;#039;Marchantia polymorpha&amp;#039;&amp;#039; (common liverwort or umbrella liverwort) may contain 5.5%, &amp;#039;&amp;#039;Eleocharis uniglumis&amp;#039;&amp;#039; (spikesedges in aquatic or mesic habitats) 6.7% and Arundinaria gigantea (also a bamboo species) may contain 8.8% silica. [http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html]&lt;br /&gt;
&lt;br /&gt;
==Arundinaria gigantea==&lt;br /&gt;
[[Image:Arundinaria_gigantea.jpg|thumb|left| Arundinaria gigantea [http://www.bamboogarden.com/Arundinaria%20gigantea%20ssp.%20gigantea.htm © Ned Jaquith]]]&lt;br /&gt;
[[Image:Arundinaria_gigantea_2.jpg|thumb|right| Arundinaria gigantea [http://galleryhip.com/arundinaria-gigantea.html]]]&lt;br /&gt;
Arundinaria gigantea leaves may contain 8.8% [http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html] or even up to 18.76% silica[http://aob.oxfordjournals.org/content/56/2/157.short] It is not a wetland specie [http://link.springer.com/article/10.1007/s11273-011-9233-3#page-1] but thrives in sandy, well-drained mineral soils.[http://www.castaneajournal.org/doi/abs/10.2179/08-038R3.1a]&lt;br /&gt;
&amp;#039;&amp;#039;Arundinaria gigantea&amp;#039;&amp;#039; (aka Giant cane, River cane or Canebrake bamboo). &amp;#039;&amp;#039;Arundinaria gigantea var. tecta&amp;#039;&amp;#039; or &amp;#039;&amp;#039;Arundinaria tecta&amp;#039;&amp;#039; (Switch cane) is smaller (up to 2 m high) and adapted to more aquatic conditions. [http://herbarium.usu.edu/webmanual/info2.asp?name=Arundinaria_gigantea&amp;amp;type=treatment] Colonies of Arundinaria tecta flower every four or five years, those of Arundinaria gigantea every 40- 50 years.[http://thekeep.eiu.edu/cgi/viewcontent.cgi?article=1180&amp;amp;context=bio_fac] &lt;br /&gt;
&lt;br /&gt;
Arundinaria gigantea is a perennial evergreen Bamboo from hard, tough rhizomes, growing up to 9 m high. Its flowers have both male and female organs and are pollinated by Wind. It grows well in sandy, loamy and clay, well-drained, moist soils. It can grow in acid, neutral and basic soils, and is indifferent of (the lack of) direct sunlight exposure. Arundaria gigantea is intolerant of drought, requiring abundant moisture and plenty of organic matter in the soil. It prefers warm, humid, damp conditions.[http://www.pfaf.org/user/Plant.aspx?LatinName=Arundinaria+gigantea] It has low calciumcarbonate tolerance.[http://www.wildflower.org/plants/result.php?id_plant=ARGI] Arundinaria gigantea may monopolize newly freed space and resources through rapid clonal growth.[http://www.esajournals.org/doi/abs/10.1890/07-1255.1] Intense rains cause transportation of sediments. Giant cane very effectively filters sediment because its dense population slows the velocity of surface runoff and because its fine root system increases soil porosity (promoting infiltration) and tightly binding soil particles.[http://link.springer.com/article/10.1007/s11270-006-3111-2#page-1] Giant cane may reduce ground water nitrate levels by 90%, through plant assimilation, denitrification and by upwelling ground water through the porous soil.[http://onlinelibrary.wiley.com/doi/10.1111/j.1752-1688.2003.tb04389.x/abstract]&lt;br /&gt;
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In bamboo stands, culms are connected to each other through rhizomes and regenerate by shooting sprouts. New sprouts attain full size within a few months.[http://link.springer.com/article/10.1007/BF02347241#page-1]&lt;br /&gt;
Propagation of giant cane can be carried out either sexually by seeds or asexually through transplanting culms. Because clusters of flowering culms are often monoclonal, sexual reproduction is often characterized by gregarious flowering, followed by death of the flowering culms and possibly of the attached rhizomes.[http://www.castaneajournal.org/doi/abs/10.2179/08-032R1.1] Hybridization occurs naturally among this species, with putative hybrids.[http://www.amjbot.org/content/97/3/471.short] Seeds are often low in viability (82–95% viable[http://www.bioone.org/doi/abs/10.3159/07-RA-054.1]), and rarely available because Giant cane rarely flowers. The seeds need to be surface sown. Compost needs to be kept moist. Germination is usually swift, but may also take up to 6 months. Maximizing seed germination offers the ability to exploit seed produced on existing canebrakes. Of the six temperature regimes tested on two populations, maximum germination occurred using roll towels, under a 35/25 or 30/20°C temperature regime.[http://www.castaneajournal.org/doi/abs/10.2179/08-042R2.1] The seedlings need to grow in light shade, in a high fertility sandy medium. [http://www.pfaf.org/user/Plant.aspx?LatinName=Arundinaria+gigantea] Levels of 5 g./L nitrogen significantly increases seedling growth when plants are grown under non-shaded conditions. Giant cane growth is enhanced with increased light levels.[http://www.castaneajournal.org/doi/abs/10.2179/08-060R3.1] Fertilizing  (N, P, and K) establishing giant cane may reduce mortality, and periodic burning can increase density and spread of this species, which should promote establishment over the long-term.[http://onlinelibrary.wiley.com/doi/10.1111/j.1526-100X.2009.00560.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
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Propagation by digging and transplanting culms and allowing for subsequent spreading of rhizomes is labor intensive and cumbersome. &lt;br /&gt;
Using rhizome cuttings to produce planting stock requires a method for the successful culm production. Surface-planted rhizomes produced 75 culms compared to&lt;br /&gt;
the 26 culms produced by buried rhizomes. Surface-planted rhizomes with 10+ internodes averaged the fewest number of internodes (7.9) needed to produce at least 1 culm. In other words, cutting rhizomes up into sections with fewer internodes (as was the case in the 2 and 4 internode pieces) resulted in fewer culms produced for a given amount of available rhizome tissue. Exposure of rhizomes to sunlight during propagation increases the number of culms that are produced, particularly for those that are greater than 10 internodes long. &lt;br /&gt;
Rhizomes become photosynthetic and thus provide energy needed to help stimulate production and the growth of culms. Exposure to light of normally shaded or light-excluded tissues may also stimulate bud break from dormant buds, as is seen in the production of epicormic shoots in trees. Relatively small rhizome sections (22 to 30 cm) offer the advantage of being able to set out more propagules with the same amount of collected plant material. Additionally, smaller rhizomes are easier to handle for outplanting.[http://www.rngr.net/publications/proceedings/2003/PDF.2004-06-08.0223/at_download/file] Macropropagation from rhizome sections derived from stock maintained in pot-in-pot container production allows for relatively easy harvest. Rhizome sections 2-3 nodes in length soaked for 60 minutes in warm water or 1000 ppm BAP (6-benzylaminopurine) showed greatest shooting yield. Individual mother plants have the potential to yield 400 viable clones.[http://www.castaneajournal.org/doi/abs/10.2179/08-042R2.1]&lt;br /&gt;
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It is recommended that rhizome propagules be yellowish, 38 to 102 cm long, with at least 10 good buds. Rhizomes from younger planted stands have higher survival in the greenhouse; having a higher number of buds may lead to a higher probability of a bud to produce a culm.&lt;br /&gt;
Potential culm size (height and diameter) is a reflection of available food reserves in the rhizomes. Using longer rhizomes provides more available non-structural carbohydrates. With the development of new shoots, carbohydrate concentration in the rhizoma decreases. Planting rhizomes directly in the field requires less effort, time, and resources than initial growing in the greenhouse.&lt;br /&gt;
Both origin (putative genotype) and stand development (collection source) influence survival and growth of culms generated from rhizomes. Obtaining stock directly from natural&lt;br /&gt;
stands tends to produce taller culms but survival was similar to rhizomes collected from plantings. While natural stand rhizomes are larger in diameter and provide taller initial culms, they do not provide the same number of buds per unit length as a plantation source. Having buds that form both new culms and new rhizomes is desirable and choosing propagules with fewer buds lowers any chances of that happening. Macropropagation success in containers can increase with rhizomes that have more buds per unit length and greater diameter.[http://www.nrs.fs.fed.us/pubs/gtr/gtr-p-24%20papers/02brendecke-p-24.pdf]&lt;br /&gt;
&lt;br /&gt;
==Low-silica grasses for Armyworms==&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Armyworm Armyworm] eggs may be used to mass-rear [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings], and its pupa and young larvae to feed [http://www.waiwiki.org/index.php?title=Tilapia tilapia]. High silica in grasses and sedges inhibits Armyworm growth. The addition of silicon to the soil increases the size, shape, number and density of spines and phytoliths in the leaves of grasses.[http://www.york.ac.uk/media/biology/images/foci/downloads/REF_5_PlantBiol_2013%20SH.pdf] And when defoliated by herbivores, silicate contents may increase 4-fold in response [http://link.springer.com/article/10.1007%2Fs00442-007-0703-5], but not nearly so much in grasses clipped with scissors.[http://www.york.ac.uk/media/biology/images/foci/downloads/REF_5_PlantBiol_2013%20SH.pdf][http://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1047&amp;amp;context=napcproceedings] Particularly in Africa the effects of grazing on silica contents are great, but not the effects of burning by fire.[http://www.ksu.edu/biology/faculty_pages/blair/Meltzer%20et%20al.%20Biogeochem%202010.pdf] Silica is deposited as phytoliths in leaves. These are harder than tooth enamel.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636089/] Phytoliths may also disrupt microbial action in the gut.[http://www.ncbi.nlm.nih.gov/pubmed/21236130]&lt;br /&gt;
Spodoptera exempta cannot easily adapt to physical defences such as silica. Silica in the leaves of grasses (2-5% of dry leaf mass [http://www.ncbi.nlm.nih.gov/pubmed/16638012]) acts as a defence. Even with short-term exposure, but also progressively impacting with time, silica reduces the conversion efficiency of food into bodymass, and the amount of nitrogen absorbed from their food, leading to reduced growth rates. &lt;br /&gt;
Exposure to silica-rich diets also caused an extremely rapid increased mandible wear.[http://www.ncbi.nlm.nih.gov/pubmed/18771503] This results in mechanical protection of resources in chlorenchyma cells. As a result of this (and of the disrupting influence of phytoliths on microbial action in the gut), less chlorophyll is released after grinding and more chlorophyll is retained after passing through the gut.[http://aob.oxfordjournals.org/content/102/4/653.full]&lt;br /&gt;
&lt;br /&gt;
Low silica grasses/sedges are Agrostis scabra (Ticklegrass, a tumbleweed ; 0.3% silica), Anthoxanthum odoratum (0.6% ; Vanilla grass in Eurasia), Arrhenatherum elatius (0.9% ; tall oatgrass in temperate Europe), Carex aquatilis (arctic and temperate water sedge), Carex flavicans, Carex subspathacea, Chasmanthium sessiliflorum (0.7% ; longleaf woodoats in temperate USA), Cymbopogon citratus (0.8% ; lemon grass in South-east Asia), Cyperus alopecuroides (aquatic tropical/temperate sedge), Dactylis glomerata {0.6% ; a cool-season perennial C3 bunchgrass), Juncus roemerianus (0.2% ; black needlerush in salt marshes and estuaries), Puccinellia phryganodes (arctic and temperate saltgrass), Scirpus tabernaemontani (0.1% ; temperate grassweed), Spartina alterniflora (0.7% ; Smooth Cordgrass in estuarine salt marshes), Spartina patens (0.8% ; saltmeadow cordgrass in brackish coastal marshes), Sporobolus cryptandrus (0.7% ; Sand dropseed in prairie), Stipa comata (0.7% ; needle/feather grass in savanna/prairie), Triticosecale spp.(0.1% ; Triticale, a wheat hybrid), Zea mays (0.8% ; maize), Zoysia japonica (0.3% ; creeping grass in temperate regions) and Setaria sphacelata (South African pigeon grass aka African bristlegrass aka Golden millet).[http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html]&lt;br /&gt;
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Grasses that utilize water more efficiently, have lower silica contents. As a result, perennial C4 grasses such as Prairie cordgrass (Spartina pectinata), Switchgrass (2.0% ; Panicum virgatum), Big bluestem (1.4% ; Andropogon gerardii) and Prairie sandreed (Calamovilfa longifolia) contain relatively little silica. [http://www.reap-canada.com/online_library/feedstock_biomass/24%20Strategies%20to.pdf]Panicum coloratum (White buffalograss; a medium-high grass) may contain 1.21% silica.[http://www.pnas.org/content/80/3/790.full.pdf] It is drought-tolerant and does well in hot climates. Other perennial C4 grasses are Yellow Indiangrass (Sorghastrum nutans, 2.0% silica) and Bermudagrass.&lt;br /&gt;
&lt;br /&gt;
==Cynodon dactylon==&lt;br /&gt;
[[Image:Cynodon_dactylon.jpg|thumb|left| Bermuda grass [http://keyserver.lucidcentral.org/weeds/data/03030800-0b07-490a-8d04-0605030c0f01/media/Html/Cynodon_dactylon_var._dactylon.htm © Sheldon Navie]]]&lt;br /&gt;
[[Image:Cynodon_dactylon_2.jpg|thumb|right| Bermuda grass [http://www.tropicalforages.info/key/Forages/Media/Html/Cynodon_dactylon.htm]]]&lt;br /&gt;
[[Image:Cynodon_dactylon_3.jpg|thumb|left| Seed-head on stem [http://keyserver.lucidcentral.org/weeds/data/03030800-0b07-490a-8d04-0605030c0f01/media/Html/Cynodon_dactylon_var._dactylon.htm © Sheldon Navie]]]&lt;br /&gt;
Bermuda grass (&amp;#039;&amp;#039;Cynodon dactylon&amp;#039;&amp;#039;) is also a perennial C4 grass. Grasses preferred by Armyworm (Spodoptera exempta) larvae are sweet grasses in the genus Cynodon. [http://www.infonet-biovision.org/default/ct/95/pests] Silica contents in Bermudagrass may be 0.15% [http://books.google.nl/books?id=or98NnK10iYC&amp;amp;pg=PA277&amp;amp;lpg=PA277&amp;amp;dq=Cynodon+dactylon+silica+content&amp;amp;source=bl&amp;amp;ots=ND2se1oT0b&amp;amp;sig=X3xoymnrrfzys6Syi-5u54uO0RM&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=sgz-U8WbA_Dy7AbioYGICg&amp;amp;ved=0CFUQ6AEwBQ#v=onepage&amp;amp;q=Cynodon%20dactylon%20silica%20content&amp;amp;f=false] or 1.5%.[http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html] Silica contents may be lower when the grass grows in sand, and higher when growing in clay.[http://www.reap-canada.com/online_library/feedstock_biomass/24%20Strategies%20to.pdf]&lt;br /&gt;
Bermuda grass is rare in Ghana, but abundant when present (up to 60 cm tall), does not tolerate shadow and prefers sandy, muddy and well drained soils. It is frequent in Mali, Ivory Coast and Nigeria. [http://portal.wikwio.org/species/show/525]. When supplemented, silica accumulation in Bermudagrass is high, but post-supplemental retention is low.[http://www.tandfonline.com/doi/abs/10.1080/01904167.2013.810248#.U_4Mv_l_vQM] &lt;br /&gt;
The higher the nitrogen content of the leaves, the higher the Armyworm fecundity. In general, Armyworm fitness is hardly affected by leave nitrogen levels.[http://link.springer.com/article/10.1007/BF00320995#page-1] Net reproduction rate of Spodoptera exempta is greater on Bermuda grass (Cynodon dactylon) and maize than on Kikuyu grass, Pennisetum clandestinum, Guinea grass, Panicum maximum and Setaria plicatilis.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=7302920&amp;amp;fileId=S1742758400001612][http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=7302920&amp;amp;fileId=S1742758400001612] Larvae offered a choice of maize and Bermudagrass showed a preference for Bermudagrass. Survival was higher on Bermudagrass than on maize but the developmental time was longer.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2377708&amp;amp;fileId=S0007485300008312]&lt;br /&gt;
&lt;br /&gt;
Bermuda grass may spread quickly via creeping stems (i.e. stolons and rhizomes).[http://keyserver.lucidcentral.org/weeds/data/03030800-0b07-490a-8d04-0605030c0f01/media/Html/Cynodon_dactylon_var._dactylon.htm] Rhizomes (root-like stems) can penetrate 40-50 cm in clay soil and 70-80 cm in sand. It is foliage dense and 10-40 cm tall (rarely to 90 cm). Bermuda grass does best in relatively fertile, well-drained soils with pH over 5.5 with mean daily temperatures above 24°C (Optimum temperature for growth is 35°C[[http://www.fao.org/ag/AGP/AGPC/doc/GBASE/DATA/PF000208.HTM]]). It occurs over an average annual rainfall range of 625-1,750 mm, up to 4,300 mm. It is not shade tolerant. It is very drought tolerant by virtue of rhizome survival through drought-induced dormancy over periods of up to 7 months. Tolerates at least several weeks of deep flooding.[http://www.tropicalforages.info/key/Forages/Media/Html/Cynodon_dactylon.htm]&lt;br /&gt;
&lt;br /&gt;
Bermuda grass may be cut when 30-40 cm tall or every 4-6 weeks, usually when in full bloom. 4 cuttings per year are possible. A stubble height of 5-10 cm gives good regrowth and maintains sward density. Renovate by ploughing or discing when sod-bound. [http://www.tropicalforages.info/key/Forages/Media/Html/Cynodon_dactylon.htm] Harvesting Bermuda grass at 4 weeks (close cut) increases crude protein in comparison with a 8-week cut. Crude protein varies from 8.3% in mature to 14% in young grass, up to 22% in nitrogen-fertilized grass. For protein production, nitrogen utilization from fertilizer (eg manure) begins to decline with 450 kg/ha. Dry matter may contain 1.1% oxalic acid. When sod-bound, renovate by ploughing or discing.[http://www.fao.org/ag/AGP/AGPC/doc/GBASE/DATA/PF000208.HTM]&lt;br /&gt;
&lt;br /&gt;
Bermuda grass may be propagated by seed or vegetatively (turfs or stolon/rhizome pieces). Normally sown at 5-10 kg/ha dehulled seed, the higher rate being used for more rapid cover. Seed is best sown onto a very well prepared, fine, weed-free seedbed and rolled in. Seedlings usually root down quickly. Improved varieties are usually planted vegetatively due to low seed set or to avoid genetic drift. Turfs or sprigs can be planted at 3.5-7 m³/ha (40-80 bu/ac) or on a 90 cm (or less) grid, into a roughly or well-prepared seedbed, but rolling is still essential. Bermuda grass responds well to improved fertility, with applications of a minimum of 10 kg/ha/month N and up to 60 kg/ha/month N necessary for moderate to high productivity.[http://www.tropicalforages.info/key/Forages/Media/Html/Cynodon_dactylon.htm]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4410</id>
		<title>Composting toilet</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Composting_toilet&amp;diff=4410"/>
		<updated>2014-12-23T20:00:14Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
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&lt;div&gt;[[Image:Urine_diversion.jpg|thumb|left| urine diversion ]]&lt;br /&gt;
[[Image:Composting_toilet.jpg|thumb|right| [http://en.wikipedia.org/wiki/Composting_toilet#mediaviewer/File:Clivus_Multrum_Composting_toilet_with_urine_diversion-dehydration.svg Clivus Multrum] adapted for solar ventilation and urine re-use&lt;br /&gt;
- 1. Humus compartment 2. Ventilation inflow 3. WC 4. Urinal 5. Urine collection 6. Manual paddles 7. Solar heating of ventilation outflow ]]&lt;br /&gt;
[[Image:Urine_diversion_2.jpg|thumb|left| urine diversion - squatting [http://en.wikipedia.org/wiki/Urine-diverting_dry_toilet#mediaviewer/File:Inside_of_the_UDDT_(5332645379).jpg]]]&lt;br /&gt;
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It is estimated that the excreta from 80 persons could fertilize a hectare. A single adult eats 250 kg of cereals per year, which may be grown on less than 250 m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;, fertilised by ~50% of the urine of that person, mixed with the waste water used by that person.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf]&lt;br /&gt;
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Composting toilets aerobically decompose waste material, by aerobic microorganisms in a highly aerated, warm (32°-49°) and moist (humidity &amp;gt;80%) environment, with ample carbon (sawdust as the sole agent will lead to clogging[http://www.lismore.nsw.gov.au/infocouncil/Open/2013/OC_13082013_ATT_EXCLUDED_WEB.HTM]). During composting, mineralization, and humification occur simultaneously and are the main processes leading to degradation of the fresh organic matter. Organic matter humification in composting occurs when lignin and its degradation products are degraded by the microorganisms.[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] Optimally, the volume of the resulting compost may be about 10% of input.[http://en.wikipedia.org/wiki/Composting_toilet] &lt;br /&gt;
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Continuous composting toilets are single container toilets that receive excrement which decomposes as it moves slowly through the container and is removed as compost from the end-product chamber. The container is permanently fitted under the toilet seat, and never has to be fully emptied as the compost can be gradually removed when it reaches the end-product chamber. [http://www.yourhome.gov.au/water/waterless-toilets] To make the fresh material move down the slope, the slope needs to be slippery, eg bathroom tiles. Natural zeolite may be used as medium for the sorption of ammonia from wastewater and subsequently as nitrogen releaser in cultures of Spirulina platensis.[http://www.sciencedirect.com/science/article/pii/S0960852413019627]&lt;br /&gt;
&lt;br /&gt;
In a non-urine-diverting system, 90% of what goes into a composting toilet is water. Compost piles need to be damp to work well, but most composting toilets suffer from too much water. (ideally 45 to 70% moisture content [http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets])&lt;br /&gt;
Ammonia may evaporate as nitrogen in a gas form, which is vented out along with carbon dioxide and water vapor.[http://www.google.com/patents/US4096592]&lt;br /&gt;
Evaporation is the primary way a composting toilet gets rid of excess water. Warmth and air flow through the unit assist the evaporation process. Every composting toilet has a vertical vent pipe to carry off moisture. Air flows across the drying trays, around and through the pile, then up the vent to the outside of the building.&lt;br /&gt;
In a cold environment, the low-grade heat produced by composting is supposed to provide sufficient updraft to carry vapor up the vent.[http://buttecreekwatershed.org/BMP/test2/Book6last.htm] In tropical conditions, passive solar heating may create an air draft.&lt;br /&gt;
&lt;br /&gt;
If operation conditions for thermophilic composting are adequate (moisture content 50 to 60%, carbon to nitrogen ratio 30 to 35 and mixing with bulking material), the temperature will rise to between 50 and 65 °C (WHO 2006). Such temperatures will effectively inactivate pathogens (WHO 2006).[http://www.sswm.info/category/implementation-tools/water-use/hardware/toilet-systems/composting-toilets] If moisture content is too high, anaerobic organisms will thrive, creating undesirable odors from anaerobic digestion.&lt;br /&gt;
&lt;br /&gt;
==Carbon / Nitrogen ratio==&lt;br /&gt;
Dry material, which contains carbon (such as dry leaves or grasses) increases composting properties. It regulates moisture and the carbon to nitrogen ratio (C/N) and enhances the composting process. The optimal carbon to nitrogen ratio for thermophilic composting is 20 to 35 (WHO 2006). The C/N ratio of humanure (135 to 270 g / person / day) is about 5 to 10 (5%-7% nitrogen) The C/N ratio of grass clippings may be 12-19 and they may contain 2.4% nitrogen. The C/N ratio of leaves may be 48 and they may contain 0.9% nitrogen.[http://www.weblife.org/humanure/chapter3_7.html] Thus 200 g humanure plus 225 g leaves may render a combined C/N ratio of 30.&lt;br /&gt;
The C/N ratio of Panicum texanum is 32.9:1.[http://www.researchgate.net/publication/24186263_Nematode_Population_Fluctuations_during_Decomposition_of_Specific_Organic_Amendments] Thus 200 g humanure plus 1379 to 1724 g. Panicum texanum may render a combined C/N raio of 30.&lt;br /&gt;
&lt;br /&gt;
Crude protein in the leaves of Giant cane (Arundinaria gigantea) is relatively low in young leaves.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=8259377&amp;amp;fileId=S1742170510000566] The C/N ratio in Giant cane leaves may be 19.4 (2.4% nitrogen and 45.8% carbon).[http://www.ars.usda.gov/SP2UserFiles/ad_hoc/19320000PosterGallery/AFGC2004_Bamboo_Final.pdf] Bamboo shoots may contain 0.6% nitrogen. Overall bamboo litter (culm, branches, leaves) may contain 1.4% nitrogen.[http://www.sciencedirect.com/science/article/pii/S0378112704006693] The carbon concentration in decomposing bamboo culms may be 50%, irrespective of decomposition level. Mean nitrogen concentration may be 1%.(going up from ~0.3% as decomposition advances) [https://www.deepdyve.com/lp/springer-journals/characteristics-of-culm-structure-and-carbon-and-nitrogen-pP0nfb7X0z/7] Bamboo biochar and wood biochar have the same C/N ratio[http://www.jesc.ac.cn/jesc_En/ch/reader/download_new_edit_content.aspx?file_no=201303180000002&amp;amp;journal_id=jesc_En] The C/N ratio of softwood may be 640, of hardwood may be 560 and that of sawdust 500, which goes down as it rots (down to 200).[http://www.weblife.org/humanure/chapter3_7.html] That is because the level of carbon (and Na, K) goes down faster than the level of nitrogen (and P).[http://link.springer.com/article/10.1007/BF00346060#page-1] Nitrogen is usually retained until 50% of the carbon remains.[http://link.springer.com/article/10.1007/s10021-004-0026-x#page-1] For the supply of carbon, one may process wood into sawdust, or one may let the chopped wood decompose naturally (with a lower C/N ratio). &lt;br /&gt;
&lt;br /&gt;
For a resulting combined C/N ratio of 30:&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 21 g bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum (C/N=32.9) + 11 g bamboo sawdust (if C/N=400)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 63 g partially decomposed bamboo wood (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum (C/N=32.9) + 24 g partially decomposed bamboo culms (if C/N=200)&lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Giant cane leaves (C/N=19.4) + 252 g partially decomposed bamboo culms (if C/N=50) &lt;br /&gt;
* 200 g humanure (C/N=7.5) + 200 g Panicum texanum (C/N=32.9) + 200 g partially decomposed bamboo culms (if C/N=50)&lt;br /&gt;
&lt;br /&gt;
==Urine==&lt;br /&gt;
[[Image:Urine-nutrients.jpg|thumb|left| Urine composition - a [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_3.jpg|thumb|right| Urine composition - c [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
[[Image:Urine-nutrients_2.jpg|thumb|left| Urine composition - b [http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf © NASA]]]&lt;br /&gt;
Excessive ammonia from urine inhibits the microbial processes in the chamber. With urine diversion, less bulking agent is needed and the C/N ratio is naturally enhanced.&lt;br /&gt;
The C/N ratio of urine (1-2 L / person / day) is about 0.8 and its nitrogen content is ~15-18%.[http://www.weblife.org/humanure/chapter3_7.html]&lt;br /&gt;
Urine can contain up to 90 percent of the nitrogen, up to 50 percent of the phosphorus, and up to 70 percent of the potassium present in human excreta.[http://www2.gtz.de/Dokumente/oe44/ecosan/en-fighting-urine-blindness-1998.pdf] It may contain 9.3 g/L urea, 1.9 g/L chloride, 1.2 g/L sodium and 0.8 g/L potassium.[http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19710023044.pdf] &lt;br /&gt;
In healthy individuals it is usually pathogen free, although undiluted it may contain inorganic salts and organic compounds at levels toxic to plants. Ureum decomposes into ammonia, which smells.&lt;br /&gt;
&lt;br /&gt;
When applying urine to pastures, 14 to 28% of the nitrogen may evaporate in 2 weeks. 50% of that will be lost within the first 24 hours.[http://www.publish.csiro.au/?paper=AR9820097] The level of nitrogen volatilized as ammonia increases with increasing temperature.[http://link.springer.com/article/10.1007/BF00335848] Urine can be used as a source of complementary nitrogen in high carbon compost. When diluted with water at a 1:8 ratio, it can be used as a fertilizer [http://www.liquidgoldbook.com/], comparable to commercial fertilizers.[http://modernfarmer.com/2014/01/human-pee-proven-fertilizer-future/] &lt;br /&gt;
&lt;br /&gt;
[http://www.waiwiki.org/index.php?title=Algae_for_Tilapia#Spirulina Spirulina platensis] may be grown on human urine.[http://link.springer.com/article/10.1631/jzus.2007.A1846#page-1] Spirulina platensis may use several nitrogen sources; most effectively sodium nitrate, ammonium nitrate and urea.[http://link.springer.com/article/10.1023/A:1011925022941#page-1] At pH 8.4, urea may be used as a source of nitrogen up to 1.5 g./L [http://link.springer.com/article/10.1007%2FBF02179776#page-1]. Best cellular growth may occur at 500 mg/l of urea.[http://www.sciencedirect.com/science/article/pii/S0960852403002359] Growth may be optimally when the urea feeding rate is slowly increased [http://www.sciencedirect.com/science/article/pii/S0044848604005952] and at 29°C. [http://onlinelibrary.wiley.com/doi/10.1002/bit.21097/abstract] Best results are obtained by continuous urea addition in exponentially increasing amount, at 30°C.[http://www.sciencedirect.com/science/article/pii/S0961953402000545] Intermittent addition of urea yields results similar to those obtained by the continuous feeding.[http://www.sciencedirect.com/science/article/pii/S0144860904000263] Spirulina platensis grows better on urea than on potassium nitrate.[http://link.springer.com/article/10.1385/ABAB:112:3:143#page-1]&lt;br /&gt;
Though Spirulina platensis may tolerate high levels of urea better [http://link.springer.com/article/10.1007/s10295-005-0025-8#page-1], it is ammonium resistant at high pH.[http://pcp.oxfordjournals.org/content/32/7/953.short] The uptake of ammonia in Spirulina platensis (supporting optimal growth) is pH dependent with an optimum at pH 9.3, proceeding at the same rates in the light and in the dark.[http://pcp.oxfordjournals.org/content/30/2/303.short] High productivity is achieved when ammonia is maintained at up to 50% of the total nitrogen.[http://www.sciencedirect.com/science/article/pii/S0960852410018213]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Armyworm&amp;diff=4400</id>
		<title>Armyworm</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Armyworm&amp;diff=4400"/>
		<updated>2014-12-21T19:51:00Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
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&lt;div&gt;African Armyworm - Spodoptera exempta&lt;br /&gt;
&lt;br /&gt;
[[Image:eggs_about_to_hatch.jpg|thumb|left| eggs about to hatch [http://www.infonet-biovision.org/default/ct/95/pests © A.M. Varela]]]&lt;br /&gt;
[[Image:hatched_larvae.jpg|thumb|left| hatched larvae]]&lt;br /&gt;
[[Image:young_larvae.jpg|thumb|left| young larvae [http://www.infonet-biovision.org/default/ct/95/pests © A.M. Varela]]]&lt;br /&gt;
[[Image:Spodoptera_exempta_3.jpg|thumb|left| final stage @ low density [http://www.ljmu.ac.uk/NSP/98748.htm]]]&lt;br /&gt;
[[Image:Spodoptera_exempta_5.jpg|thumb|left| final stage @ high density [http://icosamp.ecoport.org/armyw.html]]]&lt;br /&gt;
[[Image:Spodoptera_exempta_marching.jpg|thumb|left| marching worms [http://www.africa.upenn.edu/eue_web/worm0599.htm © UN-EUE]]]&lt;br /&gt;
[[Image:Spodoptera_exempta_pupa.jpg|thumb|left| pupa [http://article.wn.com/view/2014/06/27/NIW_Insect_of_the_day_African_armyworm_27_Jun_2014_Harper_Ad/ © NIW]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:moth.jpg|thumb|left| moth [http://dwpicture.com.au/picture.asp?picture=108221 © copyright of The DW Stock Picture Library]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Spodoptera_exempta_outbreak_map.jpg|thumb|left| outbreak map [http://www.lancaster.ac.uk/armyworm/what-is-african-armyworm]]]&lt;br /&gt;
[[Image:Praying_mantis.jpg|thumb|right| Praying mantis [http://maximpiessen.com/praying-mantids/ © MaximPiessen.com]]]&lt;br /&gt;
[[Image:Praying_mantis_2.jpg|thumb|right| Praying mantis [http://outbackjoe.com/2012/12/12/praying-mantis-eating-moth/ © OutbackJoe]]]&lt;br /&gt;
[[Image:Lacewing.jpg|thumb|right| Lacewing [http://www.kunafin.com/lacewings.htm © Kunafin]]]&lt;br /&gt;
&lt;br /&gt;
The African Armyworm (2.5 to 4 cm long) is also called Okalombo, Mysteryworm, Kommandowurm or nutgrass armyworm. It is the larval stage of a moth.&lt;br /&gt;
It does well on wet conditions and do not proliferate in dry conditions. It has been observed in East Africa and African countries south of the Sahara, Saudi Arabia, Yemen, pacific islands, south-east Asia and Australia.[http://link.springer.com/article/10.1007%2FBF00140333#page-1] In Ghana it has been recorded on maize.[http://ghana.ipm-info.org/list_insects.htm#Maize] The moths are active at night, particularly between 20:00 and 22:00, feeding on nectar. The larvae are active during daytime. &lt;br /&gt;
The moths (av. 1.6 cm long, wingspan 3 cm) live about 10 days (5 to 16 days). The female moth (only) needs water to complete oviposition.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1985.tb00019.x/abstract] (hence outbreaks coincide with rainfall after droughts) One female maximally lays about 1000 eggs in total; 500 to 600 on average. Eggs have a 0.5 cm diameter. During a period of 10 days the cream-green eggs are laid at night (before midnight, with a smaller peak at about 02·00–04·00 h.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2377708&amp;amp;fileId=S0007485300008312]) in 2 or 3 batches of 10 to 400 in one or more layers on leaves or other surfaces. The egg mass is covered with down and black hairs from the female. Right before hatching, the black head of the larvae makes the eggs turn dark. The eggs hatch in 2–5 days, usually in the early morning. The hatched larvae are greenish-translucent and just 1 mm long. As they feed on the youngest foliage of plants, they gradually become green.&lt;br /&gt;
Larval stage lasts 14 to 22 days. Pupae are formed just (2-3 cm) below the soil surface in a soil cell, in preferrably soft, moist ground. The pupae are mahogany-brown and 1 cm to 1.5 cm long. The pupal phase takes 7 to 15 days. In East Africa the total lifecycle lasts about 25 days at an average temperature of 26°C.[http://www.infonet-biovision.org/default/ct/95/pests] Spodoptera exempta may have up to 13 generations per year. &lt;br /&gt;
&lt;br /&gt;
==Density==&lt;br /&gt;
At high density (&amp;gt; 8 / L container [http://onlinelibrary.wiley.com/doi/10.1046/j.1570-7458.1998.00273.x/abstract], up to 1000 / m2) the caterpillars are black with yellow stripes, and exposed to the sun, avoiding shade. At low density, they are green or brown (and larger [http://onlinelibrary.wiley.com/doi/10.1046/j.1570-7458.1998.00273.x/abstract]), and hide in the shade. They turn black when they are 10 days old. The larvae are not just darker, but also more resistant to baculovirus infection when reared at high density (gregarious form) compared to being reared singly (solitary form). This is due to elevated vertical transmission of infection in gregarious caterpillars. [http://www.ncbi.nlm.nih.gov/pubmed/18038154] The gregarious larvae have higher nitrogen conversion efficiency on an extreme protein-limiting diet, and accumulate more lipid per amount of carbohydrate consumed on carbohydrate-deficient diets.[http://www.ncbi.nlm.nih.gov/pubmed/15670864] Though the gregarious form grows faster, the ultimate sizes of the caterpillars and moths differed little from the solitary form.[http://www.jstor.org/discover/10.2307/2402085?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104662649883] Protein levels, glyceride and carbohydrate are comparable in the two forms (gregarious and solitary).[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756&amp;amp;fileId=S0007485300012165]&lt;br /&gt;
At emergence, however, abdominal glyceride contents of gragarious moths is 2.5 to 6.1 times greater. Well hydrated gregarious moths lay twice as many eggs as well hydrated solitary phase moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756]&lt;br /&gt;
&lt;br /&gt;
==Temperature==&lt;br /&gt;
Temperature dominantly affects larval and pupal development time, larval and pupal survivorship and pupal weight. &lt;br /&gt;
The higher the temperature (34°C, 28°C or 22°C), the faster they grow.&lt;br /&gt;
Survivorship in larval and pupal stage is generally lower at 22°C. Larvae reared at high temperature exhibit lighter coloration.[http://wvsu.edu.ph/ircgs/effects-of-larval-rearing-temperature-and-host-plant-quality-on-growth-development-and-survival-of-african-armyworm-spodoptera-exempta-walker-lepidoptera-noctuidae/] larval mortality positively correlates with rainfall, and negatively with sunshine.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2377708&amp;amp;fileId=S0007485300008312]&lt;br /&gt;
&lt;br /&gt;
==Diet==&lt;br /&gt;
The Spodoptera exempta larvae feed on all types of wild grasses (Poaceae) and sedges (Cyperaceae), and on early stages of grains (incl. oat, sugarcane, maize, wheat and rice). Their preferred grasses are sweet grasses in the genus Cynodon. They grow faster (and bigger) on well watered plants (80-90% moisture content) vs water stressed plants (60-70% MC). Survivorship is generally lower on water stressed plants in very young and older larvae.[http://www.infonet-biovision.org/default/ct/95/pests] The larvae posess receptors with sensitivity for specific sugars.[http://www.ncbi.nlm.nih.gov/pubmed/858367] They prefer more carbohydrate over more protein.[http://www.ncbi.nlm.nih.gov/pubmed/15670864] But the opposite is true when virally or parasitically infected.[http://www.ncbi.nlm.nih.gov/pubmed/24033221] &lt;br /&gt;
&lt;br /&gt;
High silica in grasses and sedges inhibits Armyworm growth. Seven of the ten most important food crops are silicon accumulators (up to over 10% in Wheat and 6% in ryegrass). The [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae silica content] of specific grasses can be considerably higher than in many other plants. Spodoptera exempta cannot easily adapt to physical defences such as silica. Silica in the leaves of grasses (2-5% of dry leaf mass [http://www.ncbi.nlm.nih.gov/pubmed/16638012]) acts as a defence. Even with short-term exposure, but also progressively impacting with time, silica reduces the conversion efficiency of food into bodymass, and the amount of nitrogen absorbed from their food, leading to reduced growth rates. &lt;br /&gt;
Exposure to silica-rich diets also caused an extremely rapid increased mandible wear.[http://www.ncbi.nlm.nih.gov/pubmed/18771503] This results in mechanical protection of resources in chlorenchyma cells. As a result of this (and of the disrupting influence of phytoliths on microbial action in the gut), less chlorophyll is released after grinding and more chlorophyll is retained after passing through the gut.[http://aob.oxfordjournals.org/content/102/4/653.full]&lt;br /&gt;
&lt;br /&gt;
Setaria sphacelata (South African pigeon grass aka African bristlegrass aka Golden millet) is also a low-silica grass.[http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html] Armyworms may feed on Setaria sphacelata [http://www.tropicalforages.info/key/Forages/Media/Html/Setaria_sphacelata_var._anceps.htm], but Setaria sphacelata may be very high in oxalate (an anti-nutrient).[http://www.unboundmedicine.com/harrietlane/ub/citation/702506/An_outbreak_of_%22kikuyu_poisoning%22_in_Western_Transvaal_] [http://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740270314/abstract]&lt;br /&gt;
&lt;br /&gt;
Grasses preferred by Armyworm larvae are sweet grasses in the genus [http://www.waiwiki.org/index.php?title=Silica_in_Poaceae#Cynodon_dactylon Cynodon]. [http://www.infonet-biovision.org/default/ct/95/pests] Bermudagrass may contain 0.15% silica [http://books.google.nl/books?id=or98NnK10iYC&amp;amp;pg=PA277&amp;amp;lpg=PA277&amp;amp;dq=Cynodon+dactylon+silica+content&amp;amp;source=bl&amp;amp;ots=ND2se1oT0b&amp;amp;sig=X3xoymnrrfzys6Syi-5u54uO0RM&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=sgz-U8WbA_Dy7AbioYGICg&amp;amp;ved=0CFUQ6AEwBQ#v=onepage&amp;amp;q=Cynodon%20dactylon%20silica%20content&amp;amp;f=false] or 1.5% silica.[http://aob.oxfordjournals.org/content/96/6/1027/T1.expansion.html]&lt;br /&gt;
Net reproduction rate of Spodoptera exempta is greater on Bermuda grass (Cynodon dactylon) and maize than on Kikuyu grass, Pennisetum clandestinum, Guinea grass, Panicum maximum and Setaria plicatilis.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=7302920&amp;amp;fileId=S1742758400001612][http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=7302920&amp;amp;fileId=S1742758400001612] Larvae offered a choice of maize and Bermudagrass showed a preference for Bermudagrass. Survival was higher on Bermudagrass than on maize but the developmental time was longer.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2377708&amp;amp;fileId=S0007485300008312]  &lt;br /&gt;
&lt;br /&gt;
S. exempta may also be succesfully reared on thin lamellae. (5 g. / individual, 60% survival)[http://www.cabdirect.org/abstracts/19926782362.html;jsessionid=182A650567F0EAB465A752FB05768CEF] or dried maize-leaf powder renewed at weekly intervals. (&amp;gt; 90% survival. At 25°C eggs hatched in 3 days, larval period was 12–16 days and pupal period 10-12 days.) [http://onlinelibrary.wiley.com/doi/10.1111/j.1570-7458.1975.tb02374.x/abstract] Rearing armyworm on a simple diet with milled good quality grass as the main diet constituent will not negatively affect fecundity of adults.[http://www.researchkenya.or.ke/node/28860] The higher the nitrogen content of the leaves, the higher the Armyworm fecundity. In general, Armyworm fitness is hardly affected by leaf&amp;#039;s nitrogen levels.[http://link.springer.com/article/10.1007/BF00320995#page-1]&lt;br /&gt;
&lt;br /&gt;
==Mass rearing==&lt;br /&gt;
Armyworms may be mass reared at 24 to 25°C, 70RH and a 12h light:12h dark cycle. The armyworm performs best on a 80:20 protein:carbs diet (preferrably casein:sucrose). They cannot use maltose for optimal growth.[http://www.sith.itb.ac.id/profile/publikasi-ia/Consumption%20and%20utilization%20Spodoptera-intan-ahmad.pdf] &lt;br /&gt;
Armyworm moths may be used to rear [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] Armyworms may be used to feed fish (like [http://www.waiwiki.org/index.php?title=Tilapia tilapia]) or to rear Orius insidiosus and Agriosphodrus dohrni, and Armyworm eggs may be used to feed [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings].&lt;br /&gt;
The 2 cm long Assassin Bug (Agriosphodrus dohrni), native to China, India, Indonesia) may also be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
&lt;br /&gt;
There is an annual Insect Rearing Workshop at the United States Department of Agriculture’s laboratory at Mississippi State University, organized by the entomology department.&lt;br /&gt;
It was initiated by Dr. Davis and other experts who have over a period of more than 50 years developed an entire technology to breed large numbers of insects. During a week of lectures, labs and conversation, he and several colleagues pass on knowledge learned during a half-century of research, improvisation and hunch. The wisdom includes how to organize a lab to keep insects free of disease and contamination, how to create untainted artificial diets and when to add wild genes to keep a colony healthy.[http://www.nytimes.com/2008/01/08/science/08bugs.html?pagewanted=print&amp;amp;_r=0]&lt;br /&gt;
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[u]Nectar[/u]&lt;br /&gt;
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Newly-emerged Spodoptera exempta moth feed avidly on sources such as nectar and honey. Nectar is mainly water and sugars (55% sucrose, 24% glucose, 21% fructose), plus various aromas [http://www.ncbi.nlm.nih.gov/pubmed/22742490] (including benzyl acetone [http://www.ncbi.nlm.nih.gov/pubmed/20096581], butyric acid, ethyl 3-methylbutyrate, 2-methylbutyric acid, acetic acid, benzaldehyde, homofuraneol, gamma-dodecalactone and beta-damascenone, delta-decalactone and methional[http://www.ncbi.nlm.nih.gov/pubmed/12568564]) and other traces (incl. repellents [http://www.ncbi.nlm.nih.gov/pubmed/18755975]). The aroma profiles of flowers are influenced by 1,4-dimethoxybenzene and 1,2,4-trimethoxybenzene, of which nectar is the main source.[http://www.ncbi.nlm.nih.gov/pubmed/16195845] The ratios of sugars and water in nectars vary greatly.[http://link.springer.com/article/10.1007/s00360-011-0639-2#page-1]&lt;br /&gt;
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Major nectar sources are Maple (Acer), Tulip-tree (Liriodendron), Black locust (Robinia pseudoacacia], Basswood (Tilia), Aster, Canola (Brassica napus, Brassica rapa), Knapweed (Centaurea), Blue weed (Echium vulgare), Soybean (Glycine soja), Clover (Melilotus sp.), Smartweed (Polygonum sp.), Dandelion (Taraxacum officinale),  &lt;br /&gt;
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Spodoptera exempta moths also need water. Sucrose (and not protein / amino acids) may increase fecundity and longevity in female moths, but strictly only water is required to achieve their reproductive potential (to commence oviposition). [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1985.tb00019.x/abstract] Nectar sources are specifically important for migrating moths.[http://afrsweb.usda.gov/SP2UserFiles/person/26446/LIFE-HISTORY%20TRADE-OFFS%20IN%20INSECTS%202.pdf] Similar to bees, one may feed the moths with sugar-water instead of water.&lt;br /&gt;
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==Parasitic and viral threats==&lt;br /&gt;
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Within the Spodoptera genus, Spodoptera exempta is highly genetically diverse. One study revealed 17 different haplotypes within a single population of armyworms [http://www.ncbi.nlm.nih.gov/pubmed/23061984], with a size variation of approximately 115 to 153 kb. [http://www.ncbi.nlm.nih.gov/pubmed/20600096] This is in support of the notion that Spodoptera exempta is specifically adapted for survival at low population densities.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=7597460&amp;amp;fileId=S1742758400022633] It also explains why S. exempta (the high-density form) may readily become resistant to pathogens. Spodoptera exempta shows density–dependent phase polyphenism; it may develop different phenotypes at high and low population densities. This gives S. exempta the opportunity to invest heavily in polyphenism when this is required the most; when the likelihood of exposure to pathogens is the highest (at high-density population levels). In reared Spodoptera exempta, death is usually caused by a nuclear polyhedrosis virus.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2377708&amp;amp;fileId=S0007485300008312] &lt;br /&gt;
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* Spodoptera exempta is parasitized by Meteoridea (rare parasitic wasps). Parasitism was high (around 50%) in the low density samples (below 25 larvae/m2) and low (below 17%) in the highest density ones (about 250–1200 larvae/m2).[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2390432&amp;amp;fileId=S0007485300015054]&lt;br /&gt;
* The same is true for Baculovirus resistance; Larvae reared at a high density were found to be considerably more resistant to a nuclear polyhedrosis virus than those reared in isolation.[http://rspb.royalsocietypublishing.org/content/265/1407/1787.short] &lt;br /&gt;
&lt;br /&gt;
Nevertheless, prior to natural selection overcoming a pathogen threat, initial losses may be very high; In unsterile conditions of maintenance, almost all laboratory cultures from wild-caught female moths sustained heavy infections of a nuclear polyhedrosis virus. The appearance of the symptoms of polyhedrosis was accelerated at higher temperatures, and the incidence was higher in more crowded cultures. Transmission took place from contaminated leaves to larvae and probably from one larva to another. There was evidence also of generation-to-generation transmission through the egg.&lt;br /&gt;
The effect on polyhedra of immersion in alkali solutions was investigated; It was found that immersion for 15 minutes in 0.2% caustic potash effectively dissolved the matrix and inactivated the virus. When all equipment was sterilized in this way (or, later, with 0.4% caustic potash) apparently virus-free cultures were obtained by rearing the larvae from wild-caught females in isolation and breeding from those that survived to become adults.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2618312&amp;amp;fileId=S0007485300057072]&lt;br /&gt;
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When observing 3 generations, it appeared that most moths of the first generation migrated, after emerging from pupae in the ground, the smallest laid eggs locally. Their caterpillars were small, passive, heavily parasitized and most of the moths which developed from the survivors laid eggs locally. But vigour was regained in the third generation; this was expressed by large caterpillars of both passive and active forms, little parasites and large migrant moths.[http://www.jstor.org/discover/10.2307/2402085?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104662649883]&lt;br /&gt;
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=Spodoptera litura=&lt;br /&gt;
[[Image:Spodoptera_litura.jpg|thumb|centre| Spodoptera litura [http://en.wikipedia.org/wiki/Spodoptera_litura#mediaviewer/File:Spodoptera_litura1.jpg © KENPEI]]]&lt;br /&gt;
[[Image:Spodoptera_litura_egg_mass.jpg|thumb|left| S. litura egg mass [http://www.padil.gov.au/pests-and-diseases/pest/main/136292/2952 © Amy Carmichael - QUT]]]&lt;br /&gt;
[[Image:Spodoptera_litura_pupa.jpg|thumb|right| S. litura pupa [http://katerpillarkrishna.blogspot.nl/2012/03/spodoptera-lituraarmyworm-and.html]]]&lt;br /&gt;
[[Image:Spodoptera_litura_larvae.jpg|thumb|left| S. litura larvae [http://www.padil.gov.au/pests-and-diseases/pest/main/136292/2953# © Amy Carmichael - QUT]]]&lt;br /&gt;
[[Image:Spodoptera_litura_moth.jpg|thumb|right| S. litura moth [http://www.flickr.com/photos/13940097@N04/2985543967 © Shipher Wu]]]&lt;br /&gt;
[[Image:Spodoptera_litura_moth_2.jpg|thumb|right| S. litura moth [http://www.brisbaneinsects.com/brisbane_noctuidae/ClusterCaterpillar.htm © brisbaneinsects.com]]]&lt;br /&gt;
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Spodoptera litura, the Tropical Armyworm, is also known as Cluster caterpillar, Cotton leafworm and Tobacco cutworm. It is native to The Philippines, Malasya, Indonesia and Australia and feeds mostly on plants from the dicotyledonous family. The moth may grow 2 cm long. The green lacewing Mallada boninensis may be reared on neonates of Spodoptera litura. Compared to a diet of neonates of Helicoverpa armigera (African bollworm) and Earias vitella (Spotted bollworm), Mallada boninensis longevity and reproductive potential was better on neonates of Spodoptera litura.[http://www.indianjournals.com/ijor.aspx?target=ijor:tbs&amp;amp;volume=6&amp;amp;issue=6&amp;amp;article=033]&lt;br /&gt;
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Spodoptera moths feed on nectar, which is mainly sugar and water, virtually deficit in protein. Female moths must therefore rely on their larval diet to provision sufficient protein pool for vitellogenesis. Females caterpillars are more efficient at converting protein intake to body protein growth than males, reflecting their high protein investment for egg production. Males are more efficient at depositing lipids from carbohydrate intake, which may be related to their greater propensity to migrate.[http://www.sciencedirect.com/science/article/pii/S0022191010001952]&lt;br /&gt;
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Relative to pale individuals (low density), dark larvae (the high-density phenotype) exhibited higher haemolymph and cuticular phenoloxidase (PO) activity and a stronger melanotic encapsulation response to an artificial parasite inserted into the haemocoel.[http://onlinelibrary.wiley.com/doi/10.1111/j.0021-8790.2004.00806.x/full] PO is a key enzyme in the synthesis of the melanin pigment and implicated in resistance to a range of pathogens in the haemolymph, midgut and cuticle.[http://www.ncbi.nlm.nih.gov/pubmed/7831100][http://www.sciencedirect.com/science/article/pii/S0022201196900063]. The density of haemocytes in the haemolymph is indicative of the ability to encapsulate metazoan parasites.&lt;br /&gt;
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.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4276</id>
		<title>Tilapia</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Tilapia&amp;diff=4276"/>
		<updated>2014-12-15T17:42:18Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spacing, Word order, Grammer&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Feeding==&lt;br /&gt;
[[Image:Table_1.jpg|thumb|left| Table 1 for feeding tilapia [http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf © Alaa A. El-Dahhar]]]&lt;br /&gt;
[[Image:Chaoborus.jpg|thumb|right| Chaoborus [http://en.wikipedia.org/wiki/Glassworm#mediaviewer/File:GlasswormLateralView.JPG © Viridiflavus]]]&lt;br /&gt;
[[Image:Table_2.jpg|thumb|left| Table 2 for feeding tilapia [http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf © BFAR]]]&lt;br /&gt;
[[Image:Chironomus.jpg|thumb|right| Chironomus [http://www.thamesvalleybirds.co.uk/insect-invertebrate-macro-photographs/11156-non-biting-midge-male-probably-chironomus-plumosus.html © ladylouise62]]]&lt;br /&gt;
[[Image:Ceriodaphnia_reticulata.jpg|thumb|right| Water fleas [http://www.micromagus.net/microscopes/pondlife_cladocera.html © micromagus.net]]]&lt;br /&gt;
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Tilapia may survive on [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] or [http://www.waiwiki.org/index.php?title=Lemna_For_Fish duckweed] alone, but combined feeding results in higher growth rates. Feeding on algae. feeding most intensively occurs between 12.00 and 18.00, and in this time span the fish may consume over 3% of their bodyweight.[http://cabdirect.org/abstracts/20013100658.html;jsessionid=1F937711ACC14AC20505C4692DBC7CB3] Tilapia may also feed on insects / larvae [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2028.2004.00503.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false], such as water fleas (Ceriodaphnia &amp;lt; 1mm long) [http://link.springer.com/article/10.1007/BF00016658#page-1] (1&amp;gt; Daphnia &amp;lt;5 mm long; which cannot easily avoid predation) [http://www.int-res.com/articles/ame/25/a025p215.pdf] (or Daphniamenucoensis, which are halophylic), Chaoborus midge larva (&amp;lt; 2cm) and Chironomus midges.[http://www.nativefishlab.net/library/textpdf/17926.pdf] Superior growth is achieved by partial replacement with feed from animal origin, such as commercial fishfeed or insects (eg [http://www.waiwiki.org/index.php?title=Fruit_flies fruit flies]) and [http://www.waiwiki.org/index.php?title=Armyworm worms]. Water transparency contributes to the inclusion of insects in Nile tilapia diets.[http://www.sciencedirect.com/science/article/pii/S0075951110000204] Protein and energy of the animal-based foodstuffs are more available to Oreochromis niloticus than that of the plant-based foodstuffs.[http://www.sciencedirect.com/science/article/pii/0044848687902298] &lt;br /&gt;
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In outdoor tanks fecundity and final mean weight is higher in tilapia fed 3% bw compared to lower feeding rates.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1997.t01-1-00864.x/abstract] In adult tilapia, the optimal interval between feedings is 4 to 5 hours. At shorter intervals, eaten food will surpass the already filled stomach, and become waste. Fry may be fed 8 to 10 times a day.[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf] Energy retention is highest in juvenile Tilapia (av. 34 g.) fed 3 times daily. Protein retention is highest in fish fed 5 times daily.[https://evols.library.manoa.hawaii.edu/handle/10524/19120] Tilapia may fast up to a week with compensatory enhanced growth after refeeding.[http://www.tandfonline.com/doi/abs/10.1300/J028v18n03_02#.VEZlXvnV9JA]&lt;br /&gt;
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Table 1 (on the left) can be used by fish farmers to adjust feeding rate and frequency after determination of the hatchery constant by sampling and weighing the fish. The hatchery constant will depend on water quality, stocking density etc.[http://www.arabaqs.org/journal/vol_2/1/Text%2007%20-%2001.pdf]&lt;br /&gt;
Table 2 (on the left) is a schedule for the administration of commercial fish feed.&lt;br /&gt;
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==Protein==&lt;br /&gt;
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Protein requirement for maximum performance of tilapia larva is 35 -&amp;gt;50%, decreasing with increasing fish size.[http://jn.nutrition.org/content/111/6/1001.long][http://www.pjbs.org/pjnonline/fin988.pdf][http://www.sciencedirect.com/science/article/pii/004484869290278S] In tilapia fry the feed conversion ratio and the highest protein growth rate are highest with a 45% crude protein diet, but the protein efficiency ratio and protein productive value was highest with the 25% crude protein diet. Fingerlings and advanced juveniles showed optimum growth performance with the 35% crude protein diet.[http://www.sciencedirect.com/science/article/pii/S0044848609008849] In young tilapia, out of artificial diets with dietary protein levels of 20%, 30%, 40% and 50%, best growth and food conversion efficiency was obtained with 40% dietary protein.[http://www.sciencedirect.com/science/article/pii/0044848688900075] Maximum food conversion efficiency and growth of young tilapia was obtained using the diet containing 30% protein (compared to 20%, 40% and 50% protein), decreasing with increasing protein levels.[http://www.sciencedirect.com/science/article/pii/0044848688900075]&lt;br /&gt;
Juvenile tilapia need 30-40% protein, while adult tilapia need 20-30% dietary protein for optimum performance.&lt;br /&gt;
Tilapia broodstock need 35-40% protein for optimum reproduction, spawning efficiency, and larval growth and survival.[http://www.sciencedirect.com/science/article/pii/S0044848696013658] [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1998.00206.x/abstract][http://www.sciencedirect.com/science/article/pii/S0044848602002211]. In tilapia average 62 g. weight and 18 cm long, there was no significant increase in growth rate with increasing dietary protein levels (from 25% to 30%).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Excess protein results in unionized ammonia in the environment.[http://www.sciencedirect.com/science/article/pii/S0044848609008849]&lt;br /&gt;
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Duckweeds grown in enriched water may contain 30 to 40% protein.[http://pubs.acs.org/doi/pdf/10.1021/jf60230a040] Wolffia arrhiza (dry weight) duckweed may contain up to 40% protein.[http://www.ncbi.nlm.nih.gov/pubmed/16232450] Lemna paucicostata dry mass protein contents may range from 26% to 45%.[http://www.sciencedirect.com/science/article/pii/0304377088900794] Lemna minor grown in swine lagoon wastewater contained 32% protein.[http://www.sciencedirect.com/science/article/pii/S0960852412012291]&lt;br /&gt;
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Green [http://www.waiwiki.org/index.php?title=Algae_for_Tilapia algae] (Chlorella vulgaris, Scenedesmus obliquus) and blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) may accumalate protein from 8 to 54% as available nitrogen increases. [http://www.sciencedirect.com/science/article/pii/S0031942200803040] Spirulina platensis contains 50 to 65% protein (10% is non-protein nitrogen)[http://pubs.acs.org/doi/abs/10.1021/jf00105a022], or even up to 71.9% protein.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2012.05303.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
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Insect true protein contents may be reasonably estimated via crude protein (nitrogen x 6.25).[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Crude protein content of feeder insects (housefly, fly larvae, cockroach nymphs, tebo worms) may range from 15.5 to 19.7% [http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Total crude protein in dry matter is 32.5% in earth worms and 64.4% in adult crickets (water content &amp;gt;50%)&lt;br /&gt;
Protein contents of Armyworm (Spodoptera exempta) moths are similar in gregarious and solitary moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756]&lt;br /&gt;
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Silkworm (Bombyx mori) pupae are a rich source of high quality protein.[http://www.sciencedirect.com/science/article/pii/S0278691506000147] In silkworm larvae, two storage proteins may account for 60% of total fat body protein (80% of the soluble protein) in females, compared to only 20% of the total fat body protein in males.[http://www.sciencedirect.com/science/article/pii/0020179080900244] &lt;br /&gt;
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Mealworms (Tenebrio molitor) from commercial source contained 22.3% protein.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae but contained 76% protein on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847] Mealworms contain 53% (Tenebrio molitor) and 45% (Zophobas morio; &amp;#039;Superworm&amp;#039;) protein in dry matter.[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0051145] (Egg production in Tenebrio molitor is 640 eggs / female / year, and 1500 eggs / female / year in Zophobas morio) Yellow mealworms (Tenebrio molitor; 4.8-182.7 mg) fed on wheat flour and brewers yeast contained 24.3-27.6% protein.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] A 40% protein fish meal diet with up to 40% replacement of fish meal with mealworm (Tenebrio molitor) meal does not affect growth of catfish. Catfish fed diets with up to 80% replacement of fish meal with the worm meal still displayed good growth and feed utilization efficiency. [http://onlinelibrary.wiley.com/doi/10.1046/j.1355-557x.2001.00024.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Cuticle larval / pupal mealworm proteins are more water-soluble at low temperatures (4 to 10°C) and become more hydrophobic at 25°C.[http://www.sciencedirect.com/science/article/pii/S0965174802000450] These cuticle proteins (as in the locust) lack acidic amino acid residues, methionine, cysteine and tryptophan.[http://www.sciencedirect.com/science/article/pii/096517489400048M]&lt;br /&gt;
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The digestibility coefficients for protein in Oreochromis niloticus are: soybean meal (91%), fish meal (86%), ground corn (83%), wheat middlings (75%), poultry-offal meal (74%), and brewers grain (63%).[http://www.sciencedirect.com/science/article/pii/0044848687902298]&lt;br /&gt;
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Protease activity in Tilapia is relatively low, in comparison to Grass carp (Ctenopharyngododn idellus), Common carp (Cyprinus carpiol), silver carp (Hypophthaimichthys molitrix) and bighead carp (Aristichys nobilis Richardson).[http://en.cnki.com.cn/Article_en/CJFDTOTAL-BEAR199302007.htm] Alkaline protease activity in Oreochromis niloticus is significantly inhibited by the ingestion of soybean meal, corn gluten meal and wheat bran, in comparison to the sensitivity to protease inhibitors in seabream and sole.[http://www.sciencedirect.com/science/article/pii/S0305049199000243]&lt;br /&gt;
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==Fat==&lt;br /&gt;
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Tilapia do well on fat versus carbohydrates as a source of energy.[http://www.ncbi.nlm.nih.gov/pubmed/22004562] Excess dietary lipids, however, are readily stored in the carcass and the viscera, but are not utilized for improving growth or food utilization efficiency.[http://www.sciencedirect.com/science/article/pii/004484869190224U]&lt;br /&gt;
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Larvae of mealworms, crickets, waxworms and fruit flies (Drosophila melanogaster) on average have 30% of dry matter higher fat content than adult species.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Newly emerged Armyworm moths may (still) contain high levels of (glyceride [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1993.tb00462.x/abstract]) lipid (largely in the abdominal), the quantity depends on the larval feeding conditions. Both the Armyworm larvae and young adult moths have the capacity to accumulate lipid reserves in excess. Big female moths contain relatively much fat. The moths are able to supplement their lipid reserves following carbohydrate uptake.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.1986.tb00433.x/abstract] Gregarious larva have 2.5 to 6.1 higher abdominal glyceride contents than solitaria phase moths.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2361756] The stored fat may serve as fuel for long migratory flights.[http://www.sciencedirect.com/science/article/pii/0305049188901484] In feeder insects (house fly, fly larvae, cockroach nymphs, tebo worms), crude fat may range from 1.9% to 29.4%.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.21012/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Yellow mealworms (4.8-182.7 mg) fed on wheat flour and brewers yeast contained 12.0-12.5% fat.[http://thescipub.com/abstract/10.3844/ajabssp.2009.319.331] Mealworms (Tenebrio molitor) from commercial source contained 15% fat.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503] Mealworms (Tenebrio molitor) fed fermented grain, leaves and vegetable wastes reached half the weight of conventionally reared larvae and contained 6.4% fat on dry weight basis.[http://www.sciencedirect.com/science/article/pii/S0094576512000847]&lt;br /&gt;
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Energy content for silkworms is 674 kcal/kg and 2741 kcal/kg for waxworms.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10031/abstract]&lt;br /&gt;
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In regard to gross energy, the digestibilities in Oreochromis niloticus are: animal oil (93%), fish meal (80%), ground corn (76%), poultry-offal meal (59%), wheat middlings (58%), soybean meal (56%), and brewers grain (30%).[http://www.sciencedirect.com/science/article/pii/0044848687902298] Replacing sunflower oil (69% omega-6; 0% omega-3) with perilla oil (50 to 60% ALA/omega-3) changed fatty acid profiles in Nile tilapia, which stabilized after 20 days under the new diet. Total omega-3 in muscle tissue fat increased from 6.4% to 18.2% (almost 3-fold), particularly due to a 9.4 fold increase in ALA levels.[http://www.ncbi.nlm.nih.gov/pubmed/24262550]&lt;br /&gt;
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Spirulina may contain 7% fat (dry matter)[http://www.ejbiotechnology.info/content/vol9/issue4/full/5/]. At low nitrogen (N) levels, green algae (Chlorella vulgaris and Scenedesmus obliquus) contain 45% lipids of biomass, containing mainly 16:0 and 18:1 fatty acids. At high N levels, total lipids dropped to 20% of the dry weight, with elevated levels of polyunsaturated C16 and C18 fatty acids [[http://www.sciencedirect.com/science/article/pii/S0031942200803040] during the initial stages of growth. Towards the end of growth the main lipids contained mainly saturated fatty acids (mostly 18:1 and 16:0).[http://www.sciencedirect.com/science/article/pii/S0031942200803052] In blue-green algae (Anacystis nidulans, Microcystis aeruginosa, Oscillatoria rubescens, Spirulina platensis) lipid composition was reported not to be affected by N concentrations [http://www.sciencedirect.com/science/article/pii/S0031942200803040], but by sodium-nitrate as well as by temperature.[http://www.znaturforsch.com/ac/v59c/s59c0055.pdf] The fatty acids 18:3n−3, 18:2n−6, 18:0, 18:1 and 16:0 dominate in microalgae (eg Chlorella vulgaris, Scenedesmus abundans, Monoraphidium minitum), but are highest in Chlorella vulgaris. This difference is not reflected in their predator, Brachionus calyciflorus, and in Tilapia feeding on Brachionus calyciflorus. The Tilapia zillii larvae contained relatively much 22:6n−3, which is indicative for the capacity to elongate and desaturate dietary linoleic and linolenic acid.[http://www.sciencedirect.com/science/article/pii/S0044848699000137]&lt;br /&gt;
&lt;br /&gt;
==Carbohydrates==&lt;br /&gt;
&lt;br /&gt;
Tilapia is relatively well equipped (due to alpha-Amylases[http://www.ncbi.nlm.nih.gov/pubmed/11250550] produced by Aeromonas, Bacteroidaceae and Clostridium strains[http://www.ncbi.nlm.nih.gov/pubmed/9081303]) for utilizing carbohydrates.[http://www.ncbi.nlm.nih.gov/pubmed/23168215] Tilapia can utilize up to 46% dietary starch without growth retardation.[http://www.researchgate.net/publication/240652906_Effects_of_dietary_carbohydrate_level_on_growth_and_body_composition_of_juvenile_tilapia_Oreochromis_niloticusxO._aureus] Tilapias do better on starch than on glucose (even when the glucose diet is supplemented with chromic oxide) [http://www.ncbi.nlm.nih.gov/pubmed/7722702] In tilapia, polysaccharides have antibacterial properties, increasing resistance to infections.[http://www.ncbi.nlm.nih.gov/pubmed/9280393] &lt;br /&gt;
&lt;br /&gt;
Starch consists of amylose and amylopectin. Amylopectin is more susceptible to enzymatic cleavage. A high-dietary amylose-amylopectin ratio (&amp;gt; 0.24; as in corn starch) decreases starch digestibility and postprandial peak blood glucose and triglycerides, and inhibits feed efficiency and growth in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Amylopectin accounts for about 75% of the starch weight in reserve starch (in storage organs) [http://www.sciencedirect.com/science/article/pii/S1360138598013429] and typically more than 90% in transitory starch (in photosynthetic organs)[http://www.ncbi.nlm.nih.gov/pubmed/12068097/]. Spirodela polyrrhiza amylose content was 21%[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 25% in potatoes[http://link.springer.com/article/10.1007/BF02849767#page-1], 7-44% in barley[http://ajcn.nutrition.org/content/59/5/1075.short] and 25–28 in wheat.[http://www.sciencedirect.com/science/article/pii/S0924224405003572] &lt;br /&gt;
&lt;br /&gt;
Feeds with smaller granule size have higher starch digestibility than those with bigger granules. Spirodela polyrrhiza granules range from 1 to 8 μ [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1965.tb01814.x/abstract], compared to 2-55 μ in wheat starch [http://link.springer.com/article/10.1023/A:1022255309597#page-1], 7 to 22 µ in corn starch and 8 to 110 µ in potatoes [http://www.karger.com/Article/Abstract/196856].&lt;br /&gt;
&lt;br /&gt;
The cell walls of green algae (Chlorophyta) contain cellulose (and pectose, the outer layer) and carbohydrates are stored as starch.&lt;br /&gt;
&lt;br /&gt;
Mealworms (Tenebrio molitor) from commercial source contained 3.6% carbohydrates.[http://www.jstor.org/discover/10.2307/20094161?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21104785277503]&lt;br /&gt;
&lt;br /&gt;
Organic matter of manure (eg chicken manure [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract]) hardly contributes directly to fish growth; only indirectly through carbon incorporated in algae.[http://www.sciencedirect.com/science/article/pii/0044848690901154]&lt;br /&gt;
&lt;br /&gt;
==Chito-oligosaccharides==&lt;br /&gt;
&lt;br /&gt;
In tilapia stomach, intestine, and serum, chitinases are present. [http://www.ncbi.nlm.nih.gov/pubmed/17126584] Chitinases are enzymes required to enzymatically decompose chitin. Chitin is an amino polysaccharide; a long chain of many monosaccharide (N-acetylglucosamine) units. A chito-oligosaccharide is a short chain of just a few linked units of N-acetylglucosamine. Chitin may be split into chito-oligosaccharides. Chitin plus other composite materials (eg glycoproteins, calcium carbonate) forms the exoskeletons of insects and the flexible body wall of larva / caterpillars (eg silkworms[http://www.sciencedirect.com/science/article/pii/S0144861705005412] and fruit flies[http://www.ncbi.nlm.nih.gov/pubmed/807670]). Apparent digestibility coefficient of chitin ingested by Oreochromis niloticus from crayfish exoskeleton meal was 69.3%.[http://www.sciencedirect.com/science/article/pii/S0044848604007148] The peritrophic matrix of the tobacco hornworm (Manduca sexta) may contain 40% chitin.[http://www.sciencedirect.com/science/article/pii/0965174895000534] (Peritrophic matrices usually contain 3 to 13% chitin[http://jeb.biologists.org/content/206/24/4393.full]) Wild-caught earthworms may contain 51% chitin of dry matter.[http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract] Crustaceans may contain 12 to 20% chitin.[http://business.highbeam.com/435986/article-1G1-328419079/chitin-extraction-crustacean-shells-using-biological] Arthropod species (eg crustaceans, arachnids) with a chitin content of over 50% (of total weight) are eliminated unchanged by Tilapia zillii [http://www.sciencedirect.com/science/article/pii/0044848678900157] Insects may contain 0.3 to 5% chitin. (1.2 to 14% of dry matter)[http://www.ncbi.nlm.nih.gov/pubmed/19360565] Chitin is also an ingredient of cell walls in algae.[http://www.ncbi.nlm.nih.gov/pubmed/3910139][http://www.ncbi.nlm.nih.gov/pubmed/23559820] Dietary intake of chito-oligosaccharides can improve intestinal health, and improve tilapia resistance to infection.[http://www.ncbi.nlm.nih.gov/pubmed/25038280] Dietary intake of chito-oligosaccharides at up to 4 g/kg may considerably improve growth, survival and immune response.[http://wenku.baidu.com/view/6da4fdd250e2524de5187ec7.html] Dietary intake of chito-oligosaccharides at 3 to 5 g/kg for juvenile GIFT tilapia may increase growth performance, nonspecific immunity and promote blood lipid metabolism.[http://www.agrpaper.com/applied-research-of-chito-oligosaccharide-on-gift-tilapia-oreochromis-niloticus.htm]&lt;br /&gt;
&lt;br /&gt;
==Vitamins &amp;amp; Minerals==&lt;br /&gt;
Oxalic acid is an anti-nutrient, reducing nutrient conversion in tilapia. High calcium available to duckweeds results in higher duckweed oxalic acid contents.[http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2004.00923.x/pdf] Lower duckweed-calcium may be compensated for by higher calcium in worms fed to tilapia. In mealworms supplemented with Ca from CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; (4 to 12%), the Ca content of the mealworms increased linearly with the Ca content of the substrate during the first 24 hours and decreased after over one week, especially at the higher levels of Ca supplementation. The Ca in these mealworms was 76% as bioavailable. [http://www.bioone.org/doi/abs/10.1638/1042-7260(2000)031%5B0512:ITCCOM%5D2.0.CO%3B2] Ca concentrations of silkworms also increased in a linear fashion with increasing levels of dietary Ca. Gut-loading diets for mealworms should be supplemented to contain 9% calcium, iron (51 mg/kg) and manganese (31 mg/kg). Gut-loading diets for silkworms should be supplemented to contain 23 g calcium per kg feed.[http://onlinelibrary.wiley.com/doi/10.1002/zoo.10082/abstract]&lt;br /&gt;
&lt;br /&gt;
Insects (mealworms, waxworms, crickets, fruitflies) on average have low calcium concentrations (0.11%), but sufficient concentrations of Cu, Fe, Mg, P, and Zn to meet known requirements of domestic birds and mammals. Supermealworms (Zophobas morio) and waxworms contain deficient levels of manganese. Earthworms meet all dietary mineral requirements. [http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2361(1998)17:2%3C123::AID-ZOO7%3E3.0.CO;2-B/abstract]&lt;br /&gt;
&lt;br /&gt;
Intestinal microorganisms in Tilapia nilotica produce at least 11.2 ng of vitamin B12 per g of body weight per day. In 16-weeks without Dietary B12, fish weight increased 8-fold (normal growth), and liver-stored B12 did not decrease.[http://www.tandfonline.com/doi/abs/10.1577/1548-8659(1982)111%3C485%3AISADNO%3E2.0.CO%3B2#.VCvqCfl_s10] Magnesium absorption from the gastrointestinal tract may be highly efficient in Oreochromis mossambicus.[http://www.ncbi.nlm.nih.gov/pubmed/24194247]&lt;br /&gt;
&lt;br /&gt;
==Supplements==&lt;br /&gt;
[[Image:Allspice.jpg|thumb|right| Allspice [http://en.wikipedia.org/wiki/Allspice#mediaviewer/File:AllspiceBowl.JPG © Jonathunder]]]&lt;br /&gt;
[[Image:Babysbreath.jpg|thumb|right| Baby&amp;#039;s breath [http://en.wikipedia.org/wiki/Gypsophila_paniculata#mediaviewer/File:Gypsophila_paniculata.jpg © PiPi]]]&lt;br /&gt;
&lt;br /&gt;
* Saponin-rich plants such as Quillaja saponaria (Soap bark tree), yucca and Sapindus saponaria (wingleaf soapberry, western soapberry, jaboncillo) may increase growth of (and influence male to female ratio in) tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/22444893] Gypsophila paniculata (baby&amp;#039;s breath) and Quillaja saponaria (both high in saponins) may induce release of LH (Luteinizing hormone)[http://www.ncbi.nlm.nih.gov/pubmed/15792626] Supplementing Tilapia fry with 700 mg/kg extracted Quillaja saponin extract resulted in significantly increased growth, higher number of males, higher LH release and higher serum cholesterol.[http://www.ncbi.nlm.nih.gov/pubmed/12458187] Saponin supplementation diminishes egg production by females.[http://www.ncbi.nlm.nih.gov/pubmed/11423383] Intestinal magnesium uptake in tilapia is stimulated by chloride and inhibited by saponins (which may induce intravesicular magnesium accumulation.[http://www.ncbi.nlm.nih.gov/pubmed/8952951] In tilapia, saponins selectively permeabilize the plasma membrane.[http://www.ncbi.nlm.nih.gov/pubmed/24194248]&lt;br /&gt;
* Allspice Pimenta dioica (10 g/kg fish) acts as a growth promoter to improve feed utilization and weight gain in Mozambique Tilapia fry and acts an antimicrobial agent to enhance disease resistance during first feeding of fry.[http://www.ncbi.nlm.nih.gov/pubmed/25229484] Allspice (aka Jamaica pepper, myrtle pepper, pimenta, pimento, English pepper, newspice) is dried unripe berries of Pimenta dioica.&lt;br /&gt;
* Honey bee pollen (2.5% (w/v)) increases growth performance parameters, feed efficiency ratio and immunological, hematological and biochemical parameters in young Nile tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/25086230]&lt;br /&gt;
* A supplemental mixture (at 0.5 to 2% w/w) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle may increase lysozyme, superoxide dismutase and peroxidase activity, decrease malondialdehyde levels and reduce mortality in Aeromonas hydrophila-challenged tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24925761] 20 days administration of 1% Astragalus root (Radix astragalin seu Hedysari) plus Angelica Root (Angelicae Sinensis) at a ratio of 5:1 (w/w) increased body weight of carp relative to controls.[http://www.ncbi.nlm.nih.gov/pubmed/15123322] Another study found no effect on growth by an mixture of Astragalus membranaceus, orange peel, hawthorn, pilose asiabell root indigowoad root, taraxacum and malt.[http://www.ncbi.nlm.nih.gov/pubmed/18378466] Astragalus (aka milkvetch, locoweed) belongs to the legume family Fabaceae. Dietary Astragalus polysaccharides supplementation may improve growth performance and immune parameters of tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/24657260] Calycosin is a major isoflavonoid extracted from astragalus. It has shown to have proangiogenic effects.[http://www.ncbi.nlm.nih.gov/pubmed/21909574] Two isomeres extracted from Astragalus membranceus may stimulate proliferation in human fetal lung diploid fibroblast cells.[http://www.ncbi.nlm.nih.gov/pubmed/14659591] &lt;br /&gt;
* Survival in Streptococcus agalactiae challenged tilapia is increased by inclusion of 0.1% Sophora flavescens (root extract) in the diet.[http://www.ncbi.nlm.nih.gov/pubmed/23092731] Sophora flavescens (member of the Fabaceae family) roots contain quinolizidine alkaloids, including matrine, oxymatrine and lupeol. Matrine inhibits cell proliferation [http://www.ncbi.nlm.nih.gov/pubmed/24653570] (including proliferation of cancer cells)[http://www.ncbi.nlm.nih.gov/pubmed/24137393][http://www.ncbi.nlm.nih.gov/pubmed/24137358] Matrine prevents cardiac arrhythmias by inhibiting ouabain-induced calcium overload in guinea pigs.[http://www.ncbi.nlm.nih.gov/pubmed/24680622] Matrine has neurotoxic properties in Zebrafish (Danio rerio) larvae.[http://www.ncbi.nlm.nih.gov/pubmed/24911943]&lt;br /&gt;
&lt;br /&gt;
==Dissolved oxygen==&lt;br /&gt;
Fish take up oxygen through the limited gill surface area. At low dissolved oxygen (DO), more metabolic energy is used by the ventilatory system to maintain O&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; uptake, which limits weight gain and growth.[http://www.sciencedirect.com/science/article/pii/0044848694902364] Feed intake and growth at 5.6 ppm (mg/L) DO is significantly higher than at 3.0 ppm, particularly in big fish.[http://www.sciencedirect.com/science/article/pii/S0044848608000021] For fish over 200 grams, feed intake continuous to increase over 3.0 ppm DO (with 5.5 ppm being the incipient DO), but digestibility is higher at suboptimal DO.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2011.02882.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
DO levels in ponds increase during the night due to vertical circulation. Organic materials consume oxygen during decomposition.[http://deepblue.lib.umich.edu/bitstream/handle/2027.42/27066/0000056.pdf?sequence=1] DO levels sharply decline after feeding. Dissolved oxygen levels should be maintained above 5.0 ppm for best growth. At DO levels between 3.0–5.0 ppm feeding should be reduced, and feeding should be stopped at DO levels below 3.0 ppm. Low DO increases the toxicity of ammonia (NH&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; unionized ammonia &amp;gt; 2.0 mg/L is lethal for tilapia, growth is inhibited below 1.0 mg/L). &lt;br /&gt;
[http://agrilifecdn.tamu.edu/fisheries/files/2013/09/NCRAC-Fact-Sheet-Series-No.-114-Feeding-Tilapia-in-Intensive-Recirculating-Systems.pdf]&lt;br /&gt;
Under recirculated water conditions, mean body weight of Oreochromis aureus was higher at 6.5 ppm DO (mg/L), but food conversion ratio was best at 3.7 ppm DO.[http://www.sciencedirect.com/science/article/pii/0144860995000135]&lt;br /&gt;
Intermediate densities of phytoplankton produce higher dissolved oxygen concentrations. Dissolved oxygen levels may be raised by increasing algal growth, not necessarily by reducing algal biomass.[http://www.sciencedirect.com/science/article/pii/0044848688903572] DO concentrations at dawn is not related to manure input.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1993.tb00623.x/abstract] Low DO increases mortality due to Streptococcus infection.[http://www.sciencedirect.com/science/article/pii/S004484860000346X]&lt;br /&gt;
&lt;br /&gt;
==Salinity==&lt;br /&gt;
[[Image:Saisola_soda_3.jpg|thumb|left| Salsola soda [http://www.invasive.org/browse/detail.cfm?imgnum=5374910 © Joseph M. DiTomaso]]]&lt;br /&gt;
[[Image:Saisola_soda_2.jpg|thumb|right| Salsola soda [http://www.magicgardenseeds.com/SAL02 © MagicGardenSeeds]]]&lt;br /&gt;
&lt;br /&gt;
Oreochromis niloticus may fairly tolerate salinity up to 10 ppt [http://www.sciencedirect.com/science/article/pii/0044848685901024] Tilapia may tolerate 0.5% salinity (5 ppt).[http://region2.bfar.da.gov.ph/Downloads/grow-out%20tilapia%20final.pdf] &lt;br /&gt;
At 8 g / L salinity adult Oreochromis niloticus may be stocked at 40 fish / m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;. At 15 g/L salinity total production is more than halfed.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2006.01605.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
Energy required by Oreochromis niloticus for osmoregulation is the least in the absence of an osmotic gradient (11.6‰ salinity) [http://www.nrcresearchpress.com/doi/abs/10.1139/f69-277#.VEY_ufnV9JA] In freshwater, heating water to temperatures above 27°C is not justifiable, while at 18 or 36 ppt salinity, heating water to 32°C can maximize growth rates without lowering growth efficiency.[http://www.sciencedirect.com/science/article/pii/004484869390392C] Oreochromis mossambicus grows more rapidly in brackish water. (50% seawater)[http://www.ncbi.nlm.nih.gov/pubmed/11248987] Seawater salinity is 35 ppt.&lt;br /&gt;
&lt;br /&gt;
==Alkalinity &amp;amp; pH==&lt;br /&gt;
[[Image:Saisola_kali_3.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_3.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Saisola_kali_2.jpg|thumb|left| Salsola kali [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Salsola-kali © Jean-Paul Peltier]]]&lt;br /&gt;
[[Image:Halogeton_sativus_2.jpg|thumb|right| Halogeton sativus [http://www.teline.fr/eng/Photographs/All-Families/Amaranthaceae/Halogeton-sativus © Jean-Paul Peltier]]]&lt;br /&gt;
&lt;br /&gt;
Alkalinity is the &amp;quot;buffering&amp;quot; capacity of water to resist a change in pH (pH fluctuations). To increase pH, add sodium carbonate (Na&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;CO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;) or calcium carbonate (CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;). Sodium carbonate may be extracted (as Barilla) from the ash of plants growing in sodium-rich soils, such as Halogeton sativus (aka Salsola sativa), Salsola soda and Salsola kali. The harvested plants are dried and subsequently burned. From this ash, the (water soluble) sodium carbonate is extracted with water. This solution is boiled dry, resulting in &amp;quot;barilla&amp;quot;.[http://en.wikipedia.org/wiki/Barilla] Calcium carbonate is the main component in snailshells and eggshells, and present in rocks (particularly limestone, chalk, marble and travertine).&lt;br /&gt;
&lt;br /&gt;
To increase alkalinity, one may add sodium bicarbonate. To create sodium bicarbonate, sodium carbonate may be dissolved in water and treated with carbon dioxide. Solid sodium bicarbonate will precipitate.&lt;br /&gt;
To increase the alkalinity of 10.000 L water with 10 ppm, add 168 g. of sodium bicarbonate.[http://www.expertpool.biz/ph_alkalinity.htm]&lt;br /&gt;
Adding sodium carbonate or calcium carbonate decreases the level of free ammonium ions.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
The appropriate levels for aquaculture are 20-150 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L alkalinity and 7.4-8.2 pH.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
In general, culture waters with pH below 7.0 and total alkalinity below 50 mg /L will get benefits by liming.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] The withdrawal of CO2 by microalgae turns carbonate and bicarbonate ions into the major forms of inorganic carbon in water and increases its alkalinity. Sodium carbonate is more effective than calcium carbonate to increase water alkalinity. Adding sodium carbonate to water with 20 mg/L CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; alkalinity, increasing alkalinity up to 77 meq/L, does not affect Oreochromis niloticus growth rates. Calcium carbonate is better for tilapia growth, as increasing water hardness as well.[http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/download/8510/8510] Water alkalinity of 35 to 53 mg calcium carbonate / L water is optimum for larval tilapia growth.[http://www.ablimno.org.br/acta/pdf/acta_limnologica_contents1604E_files/Art4_16(4).pdf] For optimum Oreochromis niloticus growth, the total hardness/total alkalinity ratio needs to be greater than 1.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf] The best conditions for Oreochromis niloticus growth are 7.4-8.2 pH; total alkalinity &amp;gt; 50 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; / L; calcium hardness &amp;gt; 140 mg CaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;/L and free CO&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; &amp;lt; 7 mg/L.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
&lt;br /&gt;
Total hardness refers to the content of soluble Ca&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; and Mg&amp;lt;sup&amp;gt;2+&amp;lt;/sup&amp;gt; in the water. Tilapia need to absorb these ions from the gills for several phyiological functions. When total alkalinity is much greater than hardness, water pH may reach 11 in the mid-afternoon due to photosynthesis, because more free CO&amp;lt;sup&amp;gt;2-&amp;lt;/sup&amp;gt;&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; remains in solution.[http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/viewFile/12003/pdf]&lt;br /&gt;
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==Water flow==&lt;br /&gt;
A flow rate of 0.5 L/min per kg biomass appears to be desirable for intensive culture of tilapia.[http://www.sciencedirect.com/science/article/pii/004484869190073G]&lt;br /&gt;
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==Stocking density==&lt;br /&gt;
Tilapia need to be stocked by age group to prevent cannabalism. The risk of cannabalism increases with increasing size differences.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA465&amp;amp;dq=algae+tilapia&amp;amp;ots=BVjAbOF-zV&amp;amp;sig=aEIMIY5ekRp5adhtpwsOIqPQTMQ#v=onepage&amp;amp;q=algae%20tilapia&amp;amp;f=false] Given reasonable assumptions about production costs, the optimal final density of Oreochromis niloticus in a tank-based flow-through system is 73.7 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.ncbi.nlm.nih.gov/pubmed/23915501]&lt;br /&gt;
Combined net yield of both caged and open-pond tilapia was highest in the treatment with 50 large fish (av. 141 g.) per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;.[http://www.sciencedirect.com/science/article/pii/S0044848696013774]&lt;br /&gt;
Broodstock may be best stocked at 4 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.1999.00311.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
Red tilapia stocked in a pulsed-flow aquaculture system at densities of 10 and 20 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; had significantly higher individual weights than fish stocked at 30, 50, and 70 fish/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt;, due to limiting water quality; ammonia concentrations may be high (4 mg/L), and dissolved oxygen low (2 mg/L).[http://www.tandfonline.com/doi/abs/10.1300/J028v05n01_08#.VEY9SvnV9JA] Oreochromis niloticus fry may grow optimally when stocked at 5 fry / L and fed at 30% bodyweight / day.[http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2109.2002.00700.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Oreochromis niloticus fingerlings may be stocked at a density of 42.6/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; with a production of 18–20 kg/m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; in 210 days at a water flow rate of 1 L/min/kg fish biomass.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.1989.tb00440.x/abstract] With dissolved oxygen levels below 2 ppm (or even below 1 ppm) most of the time, stocking 3 fish per m&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; was more profitable due to increased mortaility and lower growth rates at higher stocking densities (6 and 9 fish per m&amp;lt;sup&amp;gt;2&amp;lt;/sup&amp;gt;), despite similar water qualities.[http://cals.arizona.edu/azaqua/ista/ista6/ista6web/pdf/487.pdf]&lt;br /&gt;
&lt;br /&gt;
==Growth==&lt;br /&gt;
Tilapia nilotica fingerlings grow optimally on diets containing 30-40% protein, 12-15% lipids, and 30-40% digestible carbohydrate.[http://ir.kagoshima-u.ac.jp/bitstream/10232/15661/1/AN00181784_v6-1_p56-71.pdf]&lt;br /&gt;
Where extra protein (from 25% to 30%) did not increase growth in young tilapia (av. 18 cm long, 62 g. weight), increasing feeding levels to 2% of bodyweight / day did.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2004.01201.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false]&lt;br /&gt;
&lt;br /&gt;
Feed efficiency and protein efficiency ratio are better and juvenile fish grow better at 28°C than at 22°C and 34°C. Liver gluconeogenesis and lipogenesis are increased by low temperature (22°C).[http://www.ncbi.nlm.nih.gov/pubmed/24556257] Growth and feed utilization efficiency is similar in genetically improved farmed tilapia (GIFT), genetically male Nile tilapia (GMNT) and conventional Nile tilapia (CNT).[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2007.01679.x/abstract?deniedAccessCustomisedMessage=&amp;amp;userIsAuthenticated=false] Large Oreochromis niloticus (av. 288 g.) grow less in recirculating aquaculture systems compared to flow-through systems. The opposite is true for small fish (av. 81 g.)[http://www.sciencedirect.com/science/article/pii/S0044848609008254]&lt;br /&gt;
&lt;br /&gt;
The use of tuna by-product meal as a total replacement for fish meal into tilapia diets increases oxidative stress.[http://www.ncbi.nlm.nih.gov/pubmed/25139749]&lt;br /&gt;
&lt;br /&gt;
==Masculinization==&lt;br /&gt;
Estrogen synthesis is crucial for ovarian differentiation, and transcription of the aromatase gene can be proposed as a key step in that process in fish. Treatments with an aromatase inhibitor (ATD, 1,4,6- androstatriene-3-17-dione) result in 75.3% masculinization of an all-female (XX) population in tilapia (dosage 150 mg/kg of food). The effectiveness of the aromatase inhibition by ATD is demonstrated by the marked decrease of the gonadal aromatase activity in treated animals versus control.[http://www.ncbi.nlm.nih.gov/pubmed/10471475]&lt;br /&gt;
&lt;br /&gt;
Aromatase activity as a key factor in sexual differentiation in Oreochromis niloticus.[http://www.ncbi.nlm.nih.gov/pubmed/11424212] The most sensitive time to aromatase inhibitors lies in the first week (between 7 and 14 days post hatch). Treatment with the aromatase inhibitor Fadrozole (nonsteroidal) showed a dose-dependent increase in the percentage of males from 0 to 200 mg per kg. At higher doses (200 to 500 mg / kg), the percentage of males remained more or less constant (92.5-96.0%).[http://www.ncbi.nlm.nih.gov/pubmed/10861549]&lt;br /&gt;
&lt;br /&gt;
The masculinizing actions of 17alpha-methyltestosterone (MT) are most potent at up to day 20 of age.[http://www.ncbi.nlm.nih.gov/pubmed/11694764]&lt;br /&gt;
&lt;br /&gt;
Treating female tilapia Oreochromis niloticus with methyltestosterone (at a dose of 50 mcg/g diet) resulted in 100% masculinization.[http://www.ncbi.nlm.nih.gov/pubmed/16115634] Fry may be fed a commercial diet (SRT-95) with 30 ppm α-methyltestosterone for 21 days to yield 99% males.[http://books.google.nl/books?hl=nl&amp;amp;lr=&amp;amp;id=k6UTNUgLXMIC&amp;amp;oi=fnd&amp;amp;pg=PA183&amp;amp;dq=stocking+density+oreochromis+niloticus+flow-through&amp;amp;ots=BVjC9QG1uV&amp;amp;sig=oZEoAvtMefOYOjPjqXQ-tbd_z_w#v=onepage&amp;amp;q&amp;amp;f=false]&lt;br /&gt;
&lt;br /&gt;
Treatment with tamoxifen and letrozole (200mg/kg feed) to fingerlings of O. niloticus for 60 days brought about 98.5% masculinization.&lt;br /&gt;
Treatment with 17α methyltestosterone (35 mg/kg feed) to fingerlings of O. mossambicus after 8 days post hatch for 60 days obtained 100% sex reversed males with excellent growth.[http://www.ncbi.nlm.nih.gov/pubmed/23063432]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. The critical period for elevated-temperature-induced masculinization lies between days 10 and 15 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] Higher temperatures (during 5 days) before they are 5 days old induces deformities. Masculinization is induced at elevated temperatures (28 to 32C) during 5 days after 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577] The advantage of producing faster growing males of Nile tilapia at high temperature would hardly compensate the loss of production incurred during the masculinising treatment.[http://www.sciencedirect.com/science/article/pii/S004484860000452X]&lt;br /&gt;
&lt;br /&gt;
==Feminization==&lt;br /&gt;
The critical period for low-temperature-induced feminization lies between days 5 and 10 post-hatch.[http://www.ncbi.nlm.nih.gov/pubmed/16356746] The period of maximal feminizing action of 17beta-estradiol (E(2)) upon sex ratio is before 10 days posthatching in tilapia (Oreochromis mossambicus).[http://www.ncbi.nlm.nih.gov/pubmed/11694764]&lt;br /&gt;
&lt;br /&gt;
During the restricted developmental period temperature is of great influence. Higher temperatures (during 5 days) before they are 5 days old induces deformities. Gonadal feminization is induced at lower temperatures (20°C) for 5 days before 10 days old.[http://www.ncbi.nlm.nih.gov/pubmed/10684577]&lt;br /&gt;
&lt;br /&gt;
==Parasites==&lt;br /&gt;
Tilapia may be infected with: &lt;br /&gt;
&lt;br /&gt;
* Nematodes / roundworms, such as Contracaecum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Contracaecum multipapillatum [http://www.ncbi.nlm.nih.gov/pubmed/24573461]), Paracamallanus cyathopharynx, Procamallanus laevionchus [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Rhabdochona esseniae [http://www.ncbi.nlm.nih.gov/pubmed/7596571], Rhabdochona paski [http://www.ncbi.nlm.nih.gov/pubmed/23595492], Gnathostoma spinigerum, Gnathostoma doloresi and Gnathostoma hispidum.[http://www.ajtmh.org/content/58/3/316.long] Human gnathostomiasis infection is related to ingestion of &amp;quot;ceviche&amp;quot;.[http://www.ncbi.nlm.nih.gov/pubmed/2588073]&lt;br /&gt;
* Cestodes (aka tapeworms), such as Amirthalingamia sp. (eg Amirthalingamia macracantha; higher prevalence and intensity in wet season [http://www.ncbi.nlm.nih.gov/pubmed/21972071]), and Cyclustera sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] (eg Cyclustera magna [http://www.ncbi.nlm.nih.gov/pubmed/15357392]) &lt;br /&gt;
* Trematodes / flukes (encysted metacercariae)[http://www.ncbi.nlm.nih.gov/pubmed/19795754], such as Clonorchis sinensis (human liver trematode)[http://www.ncbi.nlm.nih.gov/pubmed/22471793], Tylodelphys sp.[http://www.ncbi.nlm.nih.gov/pubmed/24407916], Stictodora tridactyla (in brackish-water fish)[http://www.ncbi.nlm.nih.gov/pubmed/2332641], Ribeiroia marini [http://www.ncbi.nlm.nih.gov/pubmed/24996], Clinostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/12015820] ((eg Clinostomum Complanatum, Clinostomum tilapiae, Euclinostomum hetereostomum [http://www.ncbi.nlm.nih.gov/pubmed/23113215]), Diplostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Neascus sp, Acanthostomum sp.[http://www.ncbi.nlm.nih.gov/pubmed/23327305], Bolbophorus sp.[http://www.ncbi.nlm.nih.gov/pubmed/21972071], Haplorchis yokogawai [http://www.ncbi.nlm.nih.gov/pubmed/21073146], Haplorchis pumilio, Haplorchis taichui, Centrocestus formosanus, Stellantchas musfalcatus, Echinochasmus japonicus [http://www.ncbi.nlm.nih.gov/pubmed/18564743], Heterophyes heterophyes, Heterophyes aequalis, Pygidiopsis genata, Stictodora sp., Phagicola sp.[http://www.ncbi.nlm.nih.gov/pubmed/18339484] (eg Phagicola ascolonga[http://www.ncbi.nlm.nih.gov/pubmed/21073146]) and Prohemostomumn vivax.[http://www.ncbi.nlm.nih.gov/pubmed/18383799] These trematodes loose their viability after 48 hours at -5°C.[http://www.ncbi.nlm.nih.gov/pubmed/19795748] The conditions of gas supersaturation (gas pressure &amp;gt; 111.2% saturation; N supersaturation, O2 undersaturation) may result in heavy monogenetic trematode infections in tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/9234554] In Thailand, fish-borne zoonotic trematode (FZT) metacercarial infections were found in Nile tilapia from cage (2.5%) and pond aquaculture systems (10%) and in wild caught fish.(53%) [http://www.ncbi.nlm.nih.gov/pubmed/24029716] In China, tilapia from nursery and grow-out ponds were sampled from monoculture, polyculture and integrated aquaculture systems, revealing a 1.5% prevalence of FZT infections (Heterophyidae and Echinostomatidae); lower than in wild caught fish. Integrated systems using animal manure and latrine wastes as fertilizer did not show a higher prevalence.[http://www.ncbi.nlm.nih.gov/pubmed/24018184] In Vietnam, the overall FZT prevalence in tilapia from wastewater ponds was 2.0%, but much higher in tilapia from farm ponds. [http://www.ncbi.nlm.nih.gov/pubmed/22471793]&lt;br /&gt;
* Monogenea (very small flatworms), such as Dactylogyrus minutus [http://www.ncbi.nlm.nih.gov/pubmed/24407916], Neobenedenia melleni [http://www.ncbi.nlm.nih.gov/pubmed/7707197][http://www.ncbi.nlm.nih.gov/pubmed/1597779], Enterogyrus cichlidarum (associated with overcrowding [http://www.ncbi.nlm.nih.gov/pubmed/7472888] and infection intensity increases with (tilapia) host size[http://www.ncbi.nlm.nih.gov/pubmed/2242958]), Gyrodactylus malalai (ectoparasite)[http://www.ncbi.nlm.nih.gov/pubmed/22807048], Gyrodactylus ergensi [http://www.ncbi.nlm.nih.gov/pubmed/19838735], Scutogyrus longicornis [http://www.ncbi.nlm.nih.gov/pubmed/25054495] and of the genus Cichlidogyrus [http://www.ncbi.nlm.nih.gov/pubmed/22436463], such as Cichlidogyrus tilapiae, Cichlidogyrus sclerosus [http://www.ncbi.nlm.nih.gov/pubmed/21791155], Cichlidogyrus dossoui [http://www.ncbi.nlm.nih.gov/pubmed/23327305], Cichlidogyrus halli [[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Cichlidogyrus thurstonae [http://www.ncbi.nlm.nih.gov/pubmed/14707506], Cichlidogyrus berradae, Cichlidogyrus revesati, Cichlidogyrus legendrei and Cichlidogyrus lemoallei.[http://www.ncbi.nlm.nih.gov/pubmed/14535345] The highest parasite number was recorded in reservoir-dwelling, and lowest in stream-dwelling tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21791155] Gyrodactylus niloticus infection may particularly become lethal (in tilapia) when followed by exposure to the bacterial pathogen Streptococcus iniae.[http://www.ncbi.nlm.nih.gov/pubmed/17394525] Low stocking density and low water temperature may result in lower monogenea parasitism rate.[http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Acanthocephala (thorny-headed worms), such as Acanthogyrus (Acanthosentis) tilapiae.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] and Polyacanthorhynchus kenyensis [http://www.ncbi.nlm.nih.gov/pubmed/12015820] In Kenya, infection rates in wild Tilapia zillii ranged from 4.1 to 77.7%.[http://www.ncbi.nlm.nih.gov/pubmed/9332980] &lt;br /&gt;
* Ciliatea (&amp;#039;small flagella&amp;#039;), such as Epistylis sp.[http://www.ncbi.nlm.nih.gov/pubmed/25054495], Paratrichodina africana [http://www.ncbi.nlm.nih.gov/pubmed/23731856], Trichodina compacta [http://www.ncbi.nlm.nih.gov/pubmed/21755153], Trichodina magna [[http://www.ncbi.nlm.nih.gov/pubmed/25054495] and Ichthyophthirius multifiliis.[http://www.ncbi.nlm.nih.gov/pubmed/22311586] Ichthyophthirius multifiliis load increases susceptibility and mortality of tilapia to Streptococcus iniae (Gram-positive bacteria) infection.[http://www.ncbi.nlm.nih.gov/pubmed/19750806] &lt;br /&gt;
* Dinophyceae (Dinoflagellata; &amp;quot;red tide&amp;quot;), such as Piscinoodinium pillulare.[http://www.ncbi.nlm.nih.gov/pubmed/25054495] (a dominant parasite in Brasilian tilapia ponds)[http://www.ncbi.nlm.nih.gov/pubmed/21755153] &lt;br /&gt;
* Myxosporea, such as Henneguya suprabranchiae [http://www.ncbi.nlm.nih.gov/pubmed/18516619], Myxobolus heterosporus [http://www.ncbi.nlm.nih.gov/pubmed/15819436] (induces total destruction of the intestine structure [http://www.ncbi.nlm.nih.gov/pubmed/12911060]), Myxobolus nounensis [http://www.ncbi.nlm.nih.gov/pubmed/11031757], Myxobolus dossoui (host size effect), Myxobolus zillii (seasonal pattern) [http://www.ncbi.nlm.nih.gov/pubmed/11383569], Myxobolus microcapsularis [http://www.int-res.com/articles/dao/44/d044p217.pdf], Myxobolus agolus, Myxobolus brachysporus, Myxobolus clarii, Myxobolus cichlidarum, Myxobolus tilapiae and Myxobolus camerounensis.[http://www.ncbi.nlm.nih.gov/pubmed/19069850]&lt;br /&gt;
* Conoidasida (unicellular), such as Goussia cichlidarum.[http://www.ncbi.nlm.nih.gov/pubmed/17885764]&lt;br /&gt;
* Phyllopharyngea (single-cell parasites), such as Chilodonella hexasticha.[http://www.ncbi.nlm.nih.gov/pubmed/22902259]&lt;br /&gt;
* Trhypochthoniellus longisetus longisetus, a mite.[http://www.ncbi.nlm.nih.gov/pubmed/21553570]&lt;br /&gt;
* Lamproglena sp. (Lernaeidae; small crustaceans) [http://www.ncbi.nlm.nih.gov/pubmed/20563429]&lt;br /&gt;
* Pentasomida (tongue worms; tiny crustaceans), such as Leiperia cincinnalis [http://www.ncbi.nlm.nih.gov/pubmed/9809320], Subtriquetra wedli [http://www.ncbi.nlm.nih.gov/pubmed/10192834] and Subtriquetra riley.[http://www.ncbi.nlm.nih.gov/pubmed/9809320]&lt;br /&gt;
* Anodontites trapesialis (the larval stage of this mussel).[http://www.ncbi.nlm.nih.gov/pubmed/12048579]&lt;br /&gt;
&lt;br /&gt;
No parasite vaccines exist.[http://www.ncbi.nlm.nih.gov/pubmed/15757476] Tilapia are relatively resistant to parasitic infections.[http://www.ncbi.nlm.nih.gov/pubmed/18537032] The prevalence of heterophyid encysted metacercariae infection decreases as fish size increases.[http://www.ncbi.nlm.nih.gov/pubmed/20644958] When dissolved oxygen, temperature, pH, nitrite and ammonia are within normal rate parasites may cause little harm to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19500461] Parasitic infection by Acanthogyrus tilapiae provokes an aggressive host response.[http://www.ncbi.nlm.nih.gov/pubmed/17106224] Tilapia may produce an induced humoral immune response against Cichlidogyrus sp.[http://www.ncbi.nlm.nih.gov/pubmed/18564741] Oreochromis mossambicus initiated a strong immune protection by direct exposure with even a small number of parasites.[http://www.ncbi.nlm.nih.gov/pubmed/21739314] Gyrodactylus numbers fluctuate independently of temperature. In anticipation of immune defenses reaching the fish surface through mucus, Gyrodactylids (Monogenea) worms progressively move away from fins with high mucus cell density to those with low density.[http://www.ncbi.nlm.nih.gov/pubmed/21840127] Tilapia may also develop resistance to Neobenedenia sp. (Monogenea) infection.[http://www.ncbi.nlm.nih.gov/pubmed/21381523]&lt;br /&gt;
&lt;br /&gt;
Freshwater mollusks in tilapia ponds, such as Melania tuberculata, Melanoides turricula, Pomacea flagellata, Haitia cubensis and Anodontiles luteola, may host various parasites that are harmful to tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/21250483] The Biomphalaria sp. snails are often found in tilapia ponds (fresh water reservoirs are their natural habitat[http://www.ncbi.nlm.nih.gov/pubmed/11100444]) and are intermediates for Schistosoma mansoni.[http://www.scielo.br/scielo.php?script=sci_arttext&amp;amp;pid=S0074-02762001000900008&amp;amp;lng=en&amp;amp;nrm=iso&amp;amp;tlng=en] Biomphalaria cf. havanensis is an intermediate host for Diplostomum ompactum, causing higher levels of infections in cultured tilapia than wild tilapia.[http://www.ncbi.nlm.nih.gov/pubmed/19452167] The snail Bulinus truncatus is an intermediate host for Clinostomum tilapiae, Euclinostomum heterostomum, Bolbophorus levantinus and Neascus-type metacercariae. The snail Melanoides tuberculata is an intermediate host for Centrocestus sp. and Haplorchis sp. The snail Melanopsis costata is an intermediate host for Pygidiopsis genata, Phagicola longa.[http://www.ncbi.nlm.nih.gov/pubmed/12911062] The snail Thiara granifera is commonly infected with Haplorchis pumilio.[http://www.ncbi.nlm.nih.gov/pubmed/12015833] Dogs, cats and pigs may also be intermediate hosts for metacercariae.[http://www.ncbi.nlm.nih.gov/pubmed/18564743]&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Mango&amp;diff=4275</id>
		<title>Mango</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Mango&amp;diff=4275"/>
		<updated>2014-12-15T14:25:44Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Comparable to oranges and clementines, mangoes are among the most all-round fruits, regarding nutrients. Mangoes are cultivated in (sub)tropical regions all over the world.&lt;br /&gt;
&lt;br /&gt;
=Low-fibre, sweet mango species=&lt;br /&gt;
If you use a juice extractor like the [http://www.thekitchn.com/product-review-hurom-slow-juic-131492 Hurom] / [http://versapers.fr/ Versapers] (masticating slow juicer), the stringy fibres from species like Tommy Atkins or Haden will clog your juicer (more so than pineapples). The chamber will fill up with long fibres that simply never get discarded, so that you need to empty and clean it after every few mangoes. With mangoes like Kent (#11 on the list), you may endlessly (&amp;gt;100 mangoes) continue juicing without having to empty or clean the chamber in between, because the short fibres are immediately discarded as pulp (as with apples, pears, clementines).&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Anwar Ratol==&lt;br /&gt;
[[Image:Anwar_Ratol.jpg|thumb|right| Anwar Ratol [http://freshfruitpakistan.blogspot.nl/2013/04/anwar-ratol-top-variety-of-mango.html © H. Khalid]]]&lt;br /&gt;
&lt;br /&gt;
* #1 (aka Anwer Rathol, Anwar Retaul or Anwar Rataul)&lt;br /&gt;
* Small size (av. 180 g), medium thick skin&lt;br /&gt;
* Matures from green to pale bright yellow &lt;br /&gt;
* Very sweet (TSS 26-28%)&lt;br /&gt;
* Fibreless flesh, medium juicy&lt;br /&gt;
* Originally from Rataul, in Bagpat district, in the province of Uttar Pradesh, India. &lt;br /&gt;
* Harvested from June to July (India and Punjab in Pakistan)&lt;br /&gt;
* Keeps well in storage&lt;br /&gt;
&lt;br /&gt;
==Alphonso==&lt;br /&gt;
[[Image:Alphonso.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #2 (aka Alphanso, Bombay)&lt;br /&gt;
* Small, roundish / oblong (av. 200 g.)&lt;br /&gt;
* Smooth, (from green to) pale greenish-yellow to deep orange peel (deepening with ripening)&lt;br /&gt;
* Smooth, firm flesh. Intense reddish-orange colour&lt;br /&gt;
* Sweet, somewhat sour (17% sugar; sugar/acid ratio: 39; TSS 21-23%) and flavoured&lt;br /&gt;
* Virtually without fibre (1%), medium juicy&lt;br /&gt;
* Originally from Western India&lt;br /&gt;
* Main production in Maharashtra and Gujarat&lt;br /&gt;
* Highest quality in Sindhudurg, Ratnagiri (Natwarlal plantation), Raigad and Thane districts&lt;br /&gt;
* Harvested from early April to early July (India) and July (some districts in Sindh, Pakistan)&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
* Tolerates high humidity&lt;br /&gt;
* Good keeping quality (up to 4 weeks at 11° C)&lt;br /&gt;
&lt;br /&gt;
==Dashehari==&lt;br /&gt;
[[Image:Dashehari.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #3 (aka Dasheri, Doshehri, Aman Dusehri or Dussehri)&lt;br /&gt;
* Small, oblong (av. 175 g)&lt;br /&gt;
* Matures from light green to light yellow, thin skin&lt;br /&gt;
* Sweet (TSS 21-22%), lemon yellow flesh&lt;br /&gt;
* Fibreless, medium juicy&lt;br /&gt;
* Very small stone&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Dasheri, Uttar Pradesh, India.&lt;br /&gt;
* Harvested from early June to July, mainly Malihabad, Uttar Pradesh (and Punjab)&lt;br /&gt;
&lt;br /&gt;
==Bagan Pali==&lt;br /&gt;
[[Image:Bagan_Pali.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #4 (aka Begun Palli, Began Phali, Banganapalli or Baganpali)&lt;br /&gt;
* Large (av. 550 g.), oval&lt;br /&gt;
* Sweet (TSS 18-20%)&lt;br /&gt;
* Fibreless, little juicy, yellow flesh &lt;br /&gt;
* Matures from dark green to yellow light green or yellow-orange&lt;br /&gt;
* Thin, smooth, shiny skin&lt;br /&gt;
* Originally from Sindh, Pakistan and/or Banganapalle in Andhra Pradesh, India.&lt;br /&gt;
* Harvested from July to August (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Sindhri==&lt;br /&gt;
[[Image:Sindhri.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #5 (aka Sindhry)&lt;br /&gt;
* Medium to large (av. 300 g., av. 400 g in Pakistan)&lt;br /&gt;
* Elongated with pointy curve&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 36; TSS 15-17%), flavoured&lt;br /&gt;
* Little fibre (3% - 5%), little juicy&lt;br /&gt;
* From green to deep matt lemon yellow thin peel (somewhat wrinkly) when ripe&lt;br /&gt;
* Deep yellow-orange flesh; soft and melting&lt;br /&gt;
* Originally from Mir Pur Khaas in Sindh&lt;br /&gt;
* Harvested from late May to July (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Chaunsa==&lt;br /&gt;
[[Image:Chaunsa.jpg|thumb|right|]]&lt;br /&gt;
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* #6 (aka Sammar Bahisht Chausa)&lt;br /&gt;
* Large (av. 350 g.), oblong&lt;br /&gt;
* Sweet (18% sugar; sugar/acid ratio: 58; TSS 18-20%)&lt;br /&gt;
* Soft, succulent flesh &lt;br /&gt;
* Medium fibre, large stone, medium juicy (delicious for eating, too stringy for the slow juicer)&lt;br /&gt;
* Thin, pale, matt yellow-greenish peel (wrinkly and slightly deeper when ripe)&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Pakistan&lt;br /&gt;
* Harvested mainly in the Rahim Yar Khan and Multan (Sahiwal) districts of Punjab in Pakistan&lt;br /&gt;
* Harvested from June to early September (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Maya==&lt;br /&gt;
&lt;br /&gt;
* #7&lt;br /&gt;
* Medium, round (av. 300 g.)&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 42; TSS 16-17%) and flavoured&lt;br /&gt;
* Red blush peel (with yellow tinge when ripe)&lt;br /&gt;
* Smooth, deep/pale yellow flesh (soft when ripe)&lt;br /&gt;
* Medium fibre, very juicy&lt;br /&gt;
* Harvested from mid-June to early July (Gambia)&lt;br /&gt;
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==Gopalbogh==&lt;br /&gt;
&lt;br /&gt;
* #8 (aka Gopal Bhogue)&lt;br /&gt;
* Small size (average 190 g.)&lt;br /&gt;
* Yellow-green peel, with small seeds&lt;br /&gt;
* Virtually without fibre&lt;br /&gt;
* Very sweet (21% sugar)&lt;br /&gt;
* Good quality mainly grown in Rajshahi region, especially in Chapainawabganj district (Bangladesh).&lt;br /&gt;
* Harvest from mid May to mid June (Bangladesh)&lt;br /&gt;
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==Sweet Elena==&lt;br /&gt;
[[Image:Sweet_Elena.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #9&lt;br /&gt;
* Large sized, ovoid (av. 350 g.)&lt;br /&gt;
* Thin, orange peel&lt;br /&gt;
* Yellow to orange flesh&lt;br /&gt;
* Little fibre&lt;br /&gt;
* Sweet (19% sugar)&lt;br /&gt;
* Originally (as a carabao variety) from Sta. Cruz, Zambales (Philippines)&lt;br /&gt;
* Harvest from March to April (Locloc, Palauig in northern Zambales, Philippines)&lt;br /&gt;
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==Ataulfo==&lt;br /&gt;
[[Image:Ataulfo.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #10 (aka Adaulfo, Adolfo, Champagne, Manila or honey mango, related to Alphonso)&lt;br /&gt;
* Small, oblong shape (av. 225 g.)&lt;br /&gt;
* With ripening, peel turns from pale green to gold-yellow (yellow to orange and wrinkles when fully ripe)&lt;br /&gt;
* Deep yellow flesh (gold when ripe), little fibre and thin pit&lt;br /&gt;
* Smooth, sweet (15% sugar), and buttery / creamy flesh&lt;br /&gt;
* Spicy flavour &lt;br /&gt;
* Best temperature is 28°C, tolerates 1100 to 3000 mm annual rainfall &lt;br /&gt;
* Originally from Chiapas, Mexico&lt;br /&gt;
* Harvest from mid-October to late December (Ecuador); February to August (Ghana)&lt;br /&gt;
* May be stored in the fridge for 2 weeks&lt;br /&gt;
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==Kent==&lt;br /&gt;
[[Image:Kent.jpg|thumb|right|]]&lt;br /&gt;
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* #11&lt;br /&gt;
* Large, rounded oval (500 to 800 g.)&lt;br /&gt;
* Smooth, waxy thick peel (green with some yellow and red; yellow increases with ripening)&lt;br /&gt;
* Soft, melting, juicy flesh (from deep yellow to deep orange when fully ripe)&lt;br /&gt;
* Limited fibre (dry weight: 0.4 - 2%), small seed&lt;br /&gt;
* Sweet (13% sugar; 14ºBrix [http://www.m.elewa.org/JAPS/2013/17.3/5.pdf])&lt;br /&gt;
* Originally from Mexico / Florida&lt;br /&gt;
* Harvest from January to March and May to August (Ghana)&lt;br /&gt;
* Large tree (up to 20 m.)&lt;br /&gt;
* No storage keeping below 13°C&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
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=Mango Cultivation=&lt;br /&gt;
==Conditions==&lt;br /&gt;
The best soil for mango trees has a pH between 5.5 and 7.5 (high pH may cause leaf tip burn). The temperature range for growing mangoes is 15 to 40°C. Mango does best within a temperature range of 24 to 27°C. The Indian race is in general intolerant of humidity (susceptible to anthracnose), the Philippine race tolerates excess moisture. The life span of each tree is approximately 100 years. Economic bearing life is 30 to 50 years.&lt;br /&gt;
Mango trees need deep soil (up to 2 m) for their root systems. Grafted saplings yield a more superior quality of fruit. Fruits are borne by mango trees from 4 to 5 years after planting. Full bearing starts at 6 to 7 years.&lt;br /&gt;
Grafted mango plants start bearing blossoms one to two years after planting, provided that there is no high humidity or intense rain during the flowering period (dryness induces flowering).&lt;br /&gt;
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==Rootstock Propagation==&lt;br /&gt;
[[Image:Mango_grafting.jpg|thumb|left| [http://www.muftisays.com/blog/ummi+taalib/2718_20-03-2012/shaikhmurid-relationship.html © ummi taalib]]] &lt;br /&gt;
[[Image:Grafted_seedling.jpg|thumb|centre| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
[[Image:Grafted_seedlings.jpg|thumb|right| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
Trees grown from mango seeds (and naturally, sexually propagated) will be very different from the tree those mangoes came from (such &amp;#039;wild mangoes&amp;#039; usually taste bad). That is why rootstocks (raised from seeds) are asexually propagated. Matured seedlings (about 60 cm tall) are grafted by inarching; a bud from a tree producing good quality mangoes is artificially attached to the rootstock. This will create a new tree with two parts: the lower (wild) part and the upper part, which is an exact copy of the tree you took this bud from. Any bud growing from the lower part needs to be pruned off immediately. The upper part needs to grow and produce branches, and eventually fruits. During the first month after planting, the young trees need to be irrigated every 3 days. During the next 2 months, they need to be irrigated once a week. After that they should not be watered for a couple of months. Each year they need a dry season of a few months. Young plants need 10 to 30 kg organic manure per plant. Don&amp;#039;t use a chemical fertilizer on young plants. Once the tree is 2 years old, you may start using a controlled release fertilizer (with higher nitrogen), like 12-5-9 or 12-8-34 (= ratios N:P:K); 1 tbsp. in 1 L warm water / meter tree height. Or just manure.  &lt;br /&gt;
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Rootstock branches should always be pruned off. To prevent viral infections, only prune using a very sharp, sterilized knife (sterilize on the spot with a propane torch).&lt;br /&gt;
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==Production technology== &lt;br /&gt;
Space mango trees about 12 meters apart. Adult mango trees need about 500-750 mm of water per year. In the wet season irrigated once per 8 to 10 days. In the dry season once per 15 to 20 days. Mango trees need dry and wet spells alternately (also kills various pathogens). Don&amp;#039;t fertilize mature trees during fruit bearing, and not too much irrigation. If during the blooming season the soil contains much nutrients (fertilizer) and water, the tree will grow, but not flower and bear fruits. &lt;br /&gt;
You may either:&lt;br /&gt;
* A: Apply 80 to 100 kg manure per year (eg humanure from [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets]), after all fruits are picked. Or:&lt;br /&gt;
* B: Apply 3-4 kg SSP, 2-3 kg Potassium Sulphate and 2-3 kg Urea before flowering. Plus 2-3 kg Urea in 2 doses after fruit setting. Or:&lt;br /&gt;
* C: Use a controlled release fertilizer once a year, such as 4-4-8 (= ratios N:P:K), after all fruits are picked (1 tbsp. in 1 L warm water / meter tree height).&lt;br /&gt;
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After fruit harvest, prune off diseased, dried, broken branches and those touching the ground. Every 3 to 4 years about 15 to 20% of old wood should be removed.&lt;br /&gt;
Picking should be done when the fruit is fully developed and mature. Natural drop of the fruit is the main indication that the fruit is ready for picking. Expected yields vary from 40 to 100 kg per tree.&lt;br /&gt;
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One cannot rear any animals (except for carnivores) on land with mango trees, as mango leaves are deadly poisonous.&lt;br /&gt;
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=Bugs=&lt;br /&gt;
&lt;br /&gt;
==Aphids==&lt;br /&gt;
[[Image:Propylea_quatuordecimpunctata.jpg|thumb|right| Predatory ladybird [http://www.colpolon.biol.uni.wroc.pl/propylea%20quatuordecimpunctata.htm]]]&lt;br /&gt;
[[Image:Aphids_on_mango.png|thumb|left| Aphids [http://www.infonet-biovision.org/default/ct/124/crops#_1804_1202 © Varela]]]&lt;br /&gt;
[[Image:Mallada_sp_2.jpg|thumb|right| Mallada sp. © Texas University]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
&lt;br /&gt;
Aphids (plant lice; Toxoptera odinae) are small (1-4 mm), living in clusters. They pierce plant tissue and suck the sap of the leaves, which may cause the leaves to bend, twist or roll up. Aphids also transmit plant viruses. Some species produce winged offspring, &amp;quot;alates&amp;quot;, that may readily disperse. Dry (and warm) weather stimulates increases in aphid numbers. Some species of ants (eg Dolichoderus cuspidatus and Formica aerate) protect and &amp;#039;farm&amp;#039; aphids, feeding on the honeydew released by the aphids&amp;#039; alimentary (gut) canals. Insects secreting honeydew may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould] &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Plant flowering plants at the boarders to attract beneficial insects. Plant &amp;#039;trap crops&amp;#039; (dill, nasturtiums, timothy grass) to monitor aphid numbers. Check the trap crops every 3 days. Infested trap crops need to be burned. Aphid&amp;#039;s natural enemies include predatory ladybirds (eg Propylea quatuordecimpunctata), hooverfly larvae, parasitic wasps, aphid midge larvae, crab spiders (Thomisidae) and lacewings (Neuroptera). Exposing aphid populations to natural predators may be successful particularly when its hot, because once bitten they release an alarm pheromone and the others all jump off the plant. Jumping aphids may experience a heat shock (&amp;gt; 35°C) since the ground may be much warmer than the shady tree. This heat shock usually sterilizes the aphids (killing the bacteria on aphids that provide nutrients essential for aphid reproduction). [http://www.sciencedaily.com/releases/2007/04/070419172046.htm]&lt;br /&gt;
&lt;br /&gt;
[u]Crab spiders (Thomisidae)[/u]; Crab spiders are ambush hunters (they don&amp;#039;t build webs) that not just eat aphids, but may also hunt ants that farm aphids. Some of these crab spiders, Xysticus sp, may however not just eat ants, but also predatory beetles (that eat aphids).&lt;br /&gt;
&lt;br /&gt;
[u]Green lacewings (Chrysopidae)[/u]; One may buy millions of lacewings (as captive-bred eggs) for biological pest control, as reared for that purpose in many countries. A female lacewing may plant over 100 eggs on plants infested with aphids. These eggs hang on a thin 1 cm long stalk, most often under a leaf. The larvae of most species of lacewings are specialised predators that eat aphids (and coccids such as mango scales, and caterpillars). These larvae sway their heads from left to right, and back, until they strike something they can eat. They have mouthparts that can pierce and suck the aphids. Many (adult) lacewing species also feed on nectar and pollen, but particularly Chrysopidae (eg Mallada signata, Mallada boninensis) are mainly predatory (one adult may eat 15 aphids per day). One may attract Chrysopidae by planting crops that attract them (mainly Asteraceae and Apiaceae), such as calliopsis (Coreopsis), cosmos (Cosmos), sunflowers (Helianthus), dandelion (Taraxacum), dill (Anethum) and angelica (Angelica). &lt;br /&gt;
&lt;br /&gt;
[u]Chemicals[/u]; If you have no other option and immediate action is required: Use Folido 50% EC at the rate of 0.45 L per 450 L water / acre. (also kills natural aphid enemies)&lt;br /&gt;
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==Fruit Flies==&lt;br /&gt;
[[Image:Fruit_fly_maggots.jpg|thumb|centre| Maggots [http://www.infonet-biovision.org/default/ct/124/crops © A. M. Varela]]]&lt;br /&gt;
[[Image:Bactrocera_invadens.jpg|thumb|left| B. invadens [https://www.flickr.com/photos/iaea_imagebank/6776806998/ © IAEA]]]&lt;br /&gt;
[[Image:Fopius_arisanus.jpg|thumb|right| F. arisanus [http://www.tephritid.com/digital.php?act=page&amp;amp;pid=28&amp;amp;id=25 © tephritid.com]]]&lt;br /&gt;
[[Image:Ceratitis_capitata.jpg|thumb|left| C. capitata [http://josedotinzulza.edublogs.org/2013/04/21/how-much-damage-can-make-a-fly/ © José Inzulza]]]&lt;br /&gt;
[[Image:Diachasmimorpha_longicaudata.jpg|thumb|right| D. longicaudata [http://www.oocities.org/brisbane_parawasps/FruitFlyParasitoid.htm]]]&lt;br /&gt;
[[Image:Ceratitis_fasciventris.jpg|thumb|left| C. fasciventris [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Oecophylla_longinoda.jpg|thumb|right| O. longinoda [http://www.alexanderwild.com/Ants/Taxonomic-List-of-Ant-Genera/Oecophylla/i-tMfJXKG © A.Wild]]]&lt;br /&gt;
[[Image:Ceratitis_cosyra.jpg|thumb|left| C. cosyra [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Jackson_trap.jpg|thumb|right| Jackson trap]]&lt;br /&gt;
[[Image:Ceratitis_rosa.jpg|thumb|left| C. rosa [http://wzciq.wenzhou.gov.cn/art/2011/1/12/art_6811_63595.html © wzciq]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis eating fly [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
In 2013, the export of mangoes from Ghana and 27 other African countries was banned from major international markets mainly due to fruit fly infestation. The European Union imposed a ban from May 1 2014, on import of mangoes from India, also due to fruit fly infestation. &lt;br /&gt;
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The fruit flies (aka peacock flies; 4-7 mm long) that may form a very severe mango cultivation pest are not the Drosophilae (2-4 mm long), but the colourful Tephritidae family (&amp;gt; 5000 species). These attack mango fruits throughout the season and lay their eggs right under the (affected) skin of mature green or ripe fruits. They have three generations and multiply very rapidly. Within one or two days the eggs hatch into white maggots. The maggots feed on the fruit, which starts to rot. After 4 to 17 days, the maggots make holes in the skin and leave the fruit to pupate. &lt;br /&gt;
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The main fruit fly pests of mango in Africa are Ceratitis capitata (Medfly), C. fasciventris, C. rosa and C. cosyra (the most destructive). C. cosyra is however rapidly displaced by Bactrocera invadens (endemic to Sri Lanka)[http://www.ncbi.nlm.nih.gov/pubmed/19610411][http://www.ncbi.nlm.nih.gov/pubmed/22251685], being predominantly a low-land pest.[http://www.ncbi.nlm.nih.gov/pubmed/16923206] B. invadens populations quickly increase with the onset of the raining season[http://www.ncbi.nlm.nih.gov/pubmed/19449630] and also lay eggs in young fruits. Fruit fly populations peak in the late dry season.[http://www.ncbi.nlm.nih.gov/pubmed/17598527]&lt;br /&gt;
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[u]Control[/u]: Collect all the fallen and affected fruits and bury them deep (&amp;gt;50 cm) into the soil. Or you can use them to breed maggots in a sealed room for fish feed. Natural enemies of tephritids include Diapriidae (tiny wasps; &amp;lt;8mm), Braconidae (also parasitoid wasps) and Oecophylla longinoda (Latreille; a weaver ant). &lt;br /&gt;
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C. cosyra, but particularly C. fasciventris and Medfly may be effectively controlled by using the host-marking pheromone.[http://www.ncbi.nlm.nih.gov/pubmed/23356072] B. invadens [http://www.ncbi.nlm.nih.gov/pubmed/24293246], the Mediterranean fruit fly (C. capitata) and the Mexican fruit fly (Anastrepha ludens) may be effectively controlled by the &amp;#039;sterile insect technique&amp;#039; (releasing overwhelming numbers of sterile insects). The effects of sterile flies are greater than those for parasitoid wasps, but they are complementary.[http://www.ncbi.nlm.nih.gov/pubmed/15568340] &lt;br /&gt;
Medfly eggs and instars (right under the peel) may be killed by immersing Ataulfo mangoes for 95 min in warm water at 47°C, also positively modifying pH (producing more palatable fruits), but can also produce a loss of firmness and weight (5%).[http://www.ncbi.nlm.nih.gov/pubmed/23356057] B. invadens is no more heat tolerant than Medfly.[http://www.ncbi.nlm.nih.gov/pubmed/21404834] &lt;br /&gt;
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[u]Bait traps[/u]; &lt;br /&gt;
Pheromone traps (85% methyl-eugenol, 5% Naled (insecticide), 10% saturated sugar) can be used for trapping male flies (Jackson trap). A three-component attractant (ammonium acetate, putrescine, and trimethylamine lures) may be used to trap female population of Medfly. (No wind: 28 m. range).[http://www.ncbi.nlm.nih.gov/pubmed/21061993] Malathion-bait sprays and Phloxine B (a xanthene dye; D&amp;amp;C Red #28) are equally effective.[http://www.ncbi.nlm.nih.gov/pubmed/11777044]&lt;br /&gt;
Of six commercial food-based attractants, Nulure captured the greatest proportion of females: 74% compared with 51-68%. But Mazoferm E802 (with or without Spinosad) and Torula yeast captured 2.4-2.6 times more females and 3.4-4.0 times more males than Nulure (also more effective than GF-120, Hymlure and Biolure).[http://www.ncbi.nlm.nih.gov/pubmed/24665714]&lt;br /&gt;
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[u]Fopius arisanus (Sonan)[/u]; F. arisanus is a tiny but most effective[http://www.ncbi.nlm.nih.gov/pubmed/12241567] and the most dominant parasitoid wasp. As parasitism increases, fruit fly infestation decreases.[http://www.ncbi.nlm.nih.gov/pubmed/17598524] Of 3 commonly used bait sprays, which all suppress fruit fly populations, Spinosad and Phloxine B bait sprays are less harmful to these wasps than Malathion bait spray. [http://www.ncbi.nlm.nih.gov/pubmed/11561838]   &lt;br /&gt;
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[u]Diachasmimorpha longicaudata[/u]; D. longicaudata (longtailed fruit fly wasp) is the most important parasitoid wasp used as part of integrated pest management programs against fruit flies such as B. invadens and Ceratitis species. They lay one or more (when hosts are scarce) eggs in fruit fly larva.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014816/] Introduction of D. longicaudata poses minimal competitive risk to F. arisanus.[http://www.ncbi.nlm.nih.gov/pubmed/12241567]&lt;br /&gt;
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[u]Oecophylla longinoda[/u]; O. longinoda is a predator weaver ant that may eat whatever insect or bug they may catch, including caterpillars, beetles, honey bees, driver ants (Dorylus nigricans Ill.) and larvae. O. longinoda is a natural enemy of fruit flies as it eats their larvae. This weaver ant is fiercely territorial and will keep other species of ants (like aphids-farming black ants) at bay. The ants continuously patrol the tree that they live in and may also catch egg-laying female fruit flies in the act, and eat them. B. invadens and C. spp numbers on Kent mangoes may be greatly reduced by Oecophylla longinoda.[http://www.ncbi.nlm.nih.gov/pubmed/17598527] &lt;br /&gt;
Weaver ants, however, also feed on honeydew. They may farm a wide range of honeydew-producing Homoptera (bugs with sucking mouthparts, including scales, aphids and mealybugs), but are mostly associated with Coccids (Saissetia; no virus vector). Only when other Coccids are scarce or unavailable, O. longinoda may farm aphids and mealybugs (unarmoured scale insects that are virus vectors). Mainly in small trees and shrubs, the mechanical damage caused by Coccids is related to the size of the attendant ant colony. Dense populations of O. longinoda are supported by interplanting with clove.[http://www.cabdirect.org/abstracts/19540500227.html;jsessionid=36A454B839F0DEEADFA83F99A7206E6D]&lt;br /&gt;
For the protection of their harvested honeydew, these weaver ants weave a translucent silken tent around living leaves. They use the silk produced by their larvae. It is claimed that pheromones produced by the weaver ants repel egglaying fruit flies. A study in Senegal, however, found that pheromones produced by O. longinoda did hardly repel egglaying females of B. invadens.[http://www.ddrn.dk/filer/forum/File/Abstract_Christina_Abel.pdf]&lt;br /&gt;
On the other hand, trees colonized by O. longinoda (in Ghana) had only 6 to 10% fly infestation, compared to 3% fly infestation in trees treated with Cypermethrin plus Dimethoate. (1614 mg per tree)[http://ugspace.ug.edu.gh/handle/123456789/2387] &lt;br /&gt;
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[u]Praying mantids[/u] may also catch and eat fruit flies. Praying mantids are opportunist predators and do not farm any insects or other bugs. Particularly young mantids may not yet be too large to prey on fruit flies (tephredids). The younger and/or smaller the praying mantids are, the smaller the insects (and any other living creature they may catch) they prey on. Praying mantid nymphs initially feed on aphids and fruit flies (drosophila), and as they grow, they skip to larger preys, such as tephritids, and eventually moths and even much bigger preys.&lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option, GF-120 (Dow chemical) may greatly (81-89% after 7-10 weeks) reduce the number of B. invadens pupae per kg mango.[http://www.ncbi.nlm.nih.gov/pubmed/19449630] Or use Dioptries 80% at the rate of 1 L in 450 L water or Malathion 57% at the rate of 0.5 L to 450 L water / acre. Broad-spectrum insecticides are most damaging to fruit flies&amp;#039; natural enemies such as parasitoid wasps.[http://www.ncbi.nlm.nih.gov/pubmed/19886446] &lt;br /&gt;
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==Long-horn Beetles==&lt;br /&gt;
[[Image:Stem_borer.jpg|thumb|left| Stem Borer [http://www.bitterrootrestoration.com/mango/stem-borer-batocera-rufomaculata.html ©]]]&lt;br /&gt;
[[Image:Dastarcus_helophoroides.jpg|thumb|right| D. helophoroides[https://www.ffpri.affrc.go.jp/labs/seibut/bcg/bcg00284.html ©]]]&lt;br /&gt;
[[Image:Batocera_rufomaculata_2.jpg|thumb|left| Batocera rufomaculata [http://agritech.tnau.ac.in/crop_protection/crop_prot_crop_insectpest%20_Mango_pest&amp;amp;disease.html © TNAU]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Batocera_rubus.jpg|thumb|left| Batocera rubus [http://163.20.112.34/~afu/77v.htm © ]]]&lt;br /&gt;
[[Image:Batocera_baby.jpg|thumb|left| baby Batocera [https://www.flickr.com/photos/bondingtool/14291758294 © Samantha - thebondingtool.com]]]&lt;br /&gt;
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* [u]The (red-spotted) Stem Borer (Batocera rufomaculata)[/u], is a large long-horned beetle (up to 5 cm long body). It cuts incisions on the bark and lays its eggs (up to 200) inside the cuts or cracks of the (damaged) tree bark, trunk or cavities (eg exposed roots). The beetles feed on the bark of living trees and eat the green of young shoots. The grub of the stem borer pupates and feeds inside the stem of mango trees (and other fruit trees, including durian, figs, avocado, jackfruit and cashew nuts), creating a tunnel towards the surface (up to 2 cm in diameter), eventually becoming a beetle. Sap (from the tree) and frass (or coarse sawdust, from the larvae) may drip from the holes. Older larvae may also tunnel deeper in the trunk or branches. This causes drying of branches, and the tree may die. The incidence of infestation of trunk borer is highly influenced by maximum temperature. Infestation is highest when temperature and humidity are high.[http://www.ncbi.nlm.nih.gov/pubmed/24498801] For reproduction, it needs to lay its eggs in a stem that is at least 7 cm in diameter. The Stem Borer has been observed in the west coast of Africa, Madagascar, Mauritius, Réunion, Seychelles, Turkey, Israel, Syria, Iraq, Iran, Pakistan, India, Bangladesh, Sri Lanka, China, Nepal, Thailand, Indonesia, Malaysia and the West Indies. Adults may live up to 4 months. Full grown larva may be 8 to 10 cm long, and may live up to a year, causing a lot of damage. Larval and prepupal + pupal stage lasts about 280 and 24-29 days.[http://www.actahort.org/books/787/787_41.htm]&lt;br /&gt;
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* [u]The (yellow-spotted) Mango Longhorned Beetle (Batocera rubus)[/u] is also a wood borer and about equally large as the Stem Borer. Full grown larvae are 6 to 8 cm long. It attacks various fruit trees, including mango, breadfruit and figs, but also bonsay trees, rubber trees and freshly felled timber. Batocera rubus has been observed in Pakistan, India, Bangladesh, China, Cambodia, Indonesia, Malaysia, Phillipines, Thailand, Taiwan and Vietnam.&lt;br /&gt;
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[u]Natural enemies[/u]; Tiny parasitoid wasps such as Oobius agrili sp.n. (1 mm long), Spathius agrili, Avetianella batocera, Tetrastichus planipennisi and Avetianella xystrocerae sp.n (1.5 mm long) have been shown effective against (the larvae of) some specific wood-boring beetles (Agrilus planipennis, Agrilus sexsignatus and Xystrocera globosa)[http://www.emeraldashborer.info/files/Zhang%20et%20al%202005%20Oobius_Leah.pdf][http://www.fs.fed.us/nrs/pubs/other/2013/2013_McManus_FHTET-13-01.pdf]. Batocera rufus (the mango longhorn beetle), is parasitized (its eggs) by  Louricia ovivora, gen. et sp. n.(a moth), and Ooencyrtus batocerae, sp. n.(a wasp) [http://cabdirect.org/abstracts/19360501471.html;jsessionid=A9E2E64A372836E8E24F2B4E97287DC9] Oobius batocera is an opportunistic parasitic wasp that parasitizes various insect eggs, including beetle eggs. [http://www.nhm.ac.uk/research-curation/research/projects/chalcidoids/database/synonyms.dsml?FamilyCode=EE&amp;amp;ValFamTrib=&amp;amp;VALGENUS=Oobius&amp;amp;VALSPECIES=batocerae&amp;amp;VALAUTHOR=(Ferri%E8re)&amp;amp;VALDATE=1936&amp;amp;ValidAuthBracket=false&amp;amp;HOMCODE=0&amp;amp;&amp;amp;listPageURL=listChalcids.dsml%3FSuperfamily%3DChalcidoidea%26Family%3DEncyrtidae%26Genus%3DOobius] &lt;br /&gt;
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There are also various mites that parasitize Batocera longhorned beetles.[http://www.jstor.org/discover/10.2307/3225250?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21103843600301] Tetrapolipus diastocerae n. sp., Tetrapolipus afrobatocerae n. sp., Tetrapolipus ramarajui n. sp. and Tetrapolipus seemani n. sp. are described. Tetrapolipus afrobatocerae has been observed in Africa, Tetrapolipus hunteri in Australia.[http://www.researchgate.net/publication/233099056_The_Genus_Tetrapolipus_(Acari_Podapolipidae)_Parasites_of_Tropical_and_Semitropical_Cerambycidae_(Coleoptera)_New_Distribution_Records_and_Descriptions_of_Four_New_Species] Tetrapolipus sulawesiensis is a mite that parasitizes Batocera herculus.[http://d.wanfangdata.com.cn/periodical_dwfl200202010.aspx] &lt;br /&gt;
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[u]Dastarcus helophoroides[/u]; This predator/parasitoid beetle (originally from China and Japan) is an important natural enemy of longhorned beetles. In the larval stage they attack various long horn beetles, including Batocera horsfieldi and Anoplophora glabripennis. Populations are attracted specifically by the frass of their original host. Eggs are laid twice a year, near the host entrance hole, frass-extrusion holes or larval tunnel walls, guided by semiochemicals. The freshly hatched (at about 21°C) first instars (0.7 mm in length) have legs, and actively move to the host. They bite and paralyze the host, and then suck hemolymph and degenerated tissues from the dead or paralyzed prey. Adults can live over 4 years. [http://link.springer.com/article/10.1007%2Fs10526-009-9224-y#page-1] Mass production is feasible if hatched larvae are fed cerambycid larvae until they are about 8 mm in length, and subsequently reared on artificial diet (silkworm pupa powder, dry yeasts, yeast extract, sucrose, peptone, squid liver oil, preservatives and distilled water). The full grown larvae are about 18 mm in length.[http://link.springer.com/article/10.1023%2FA%3A1009936609401#page-1] Adults don&amp;#039;t readily disperse; they need to be released on each tree separately. And even if you release them at 1.2 m height, their effectiveness decreases with increasing height in the tree. Yet their effectiveness may be very high; up to 85% host mortality. [https://www.ffpri.affrc.go.jp/labs/kanko/401-1.pdf]&lt;br /&gt;
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[u]Control[/u]; The stem borer is active during the night and attracted by light, so that light traps may be effective. Bacillus thuringiensis is a bacterium commonly used as a biological pesticide, and naturally present in the gut of various caterpillars. This bacterium is toxic to many insects. A specific strain of this bacterium, Bacillus thuringiensis ZQ-89 is also toxic to adult long-horned beetles.[http://www.ncbi.nlm.nih.gov/pubmed/21332894] Prevent the stem borer from laying eggs in the bark by applying a thick layer of coaltar on the stem. Or one may paint (at 0.5%) adult mango trees with monocrotophos or phosalone.[http://www.cabdirect.org/abstracts/19790379463.html] One may also specifically apply the insecticides to the potentially affected sites (cracks and cavities), and to twigs and young shoots to deter feeding by adults. One may poke with a hard wire to physically damage, and/or inject insecticides where larvae are suspected. Chloroform, ethyl acetate, Metasystox [demeton-S-methyl] and a mixture of petroleum and kerosene oil (at 5 ml/bore) and ethylene dibromide (at 3 ml/bore) gave 100% mortality of B. rufomaculata larvae.[http://www.cabdirect.org/abstracts/19881107545.html] Plaster the holes with wet clay, aluminium phosphide tablets (3 g/hole) and dichlorvos (0.1% spray).[http://www.cabdirect.org/abstracts/19790379463.html] Among the chemical treatments, Imidacloprid 17.8% SL, Thiamethoxame 25% WG was found best in management of mango stem borer.[http://www.gifre.org/admin/papers/gjbahs/management-vol-2-4-gjbahs.pdf]&lt;br /&gt;
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==Mango Hoppers==&lt;br /&gt;
[[Image:Idioscopus_niveosparsus.jpg|thumb|left| I. niveosparsus [http://163.20.112.34/~afu/641x.htm ©]]]&lt;br /&gt;
[[Image:Mallada_basalis.jpg|thumb|right| Mallada sp. [http://3insect.blogspot.nl/2012/06/mallada-basalis.html © Chen KunTsan]]]&lt;br /&gt;
[[Image:Idioscopus_clypealis.jpg|thumb|left| I. clypealis [http://163.20.112.34/~afu/641y.htm ©]]]&lt;br /&gt;
[[Image:Mallada_signata_3.jpg|thumb|right| Mallada signata [http://www.oocities.org/brisbane_lacewings/Chrysopidae.htm © Keith Power]]]&lt;br /&gt;
[[Image:Idioscopus_nitidilus.jpg|thumb|left| I. nitidilus [http://tech.groups.yahoo.com/group/pestnet/message/6414 ©]]]&lt;br /&gt;
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Mango hoppers:&lt;br /&gt;
* Idioscoynio chypeabis&lt;br /&gt;
* Idioscopus niveosparsus&lt;br /&gt;
* Idioscopus clypealis&lt;br /&gt;
* Idioscopus nitidulus&lt;br /&gt;
* Amritodus atkinsoni aka mango leafhopper&lt;br /&gt;
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Mango hopper nymphs as well as adults puncture and suck the sap of tender parts of the mango plant. Heavy drainage will cause the leaves to curl and dry. Infested flowers will shrivel and turn brown. Hoppers also secrete honeydew, which may cause sooty mould, inhibiting the leaves from obtaining energy from daylight. Sufficient spacing of trees and pruning of overlapping branches are essential in preventing hopper infestation, as shade (and high humidity) favour hopper multiplication.&lt;br /&gt;
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[u]Natural enemies[/u]:&lt;br /&gt;
Parasites: Aprostocetus sp., Gonatocerus sp. and Polynema sp. parasitize eggs of Idioscopus clypealis and Idioscopus nitidulus.[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
A preparation of the fungus Beauveria bassiana is also somewhat effective against mango hoppers. Chrysopa lacciperda [Plesiochrysa lacciperda] and Mallada boninensis predate on on nymphs of I. clypealis, I. nitidulus and Amritodus atkinsoni.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9]&lt;br /&gt;
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[u]Mallada boninensis[/u]; This green lacewing is an effective [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=5&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFQQFjAE&amp;amp;url=http%3A%2F%2Fwww.researchjournal.co.in%2Fonline%2FIJAS%2FIJAS%25203(2)%2F3_A-164-166.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNGZ9IidKvjO6s1zNs0HvbUMpKKPBg] generalist predator, feeding on mealy bugs (Mani and Krishnamoorthi, 1987), white flies (Selvakumaran et aI., 1996), bollworms and aphids (Kabissa et al., 1996) and on nymphs of Aphis gossypii, Aphis craccivora and Rhopalosiphum maidi [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=3&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEAQFjAC&amp;amp;url=http%3A%2F%2Fwww.ets-staffing.com%2Fcurrentbiotica%2Fjournals6-issueiii%2Fcb-6-3-short-notes-3.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNETNWfEOrZznoJjj0ZrFFOINJ2liw], blackflies, psylla and leaf miner. Green lacewings are recommended for the integrated pest management programme (Nehare et al., 2004). M. boninensis feeds well on Corcyra cephalonica eggs[http://cabdirect.org/abstracts/20113350526.html;jsessionid=69EA3BE15508EF9C029CE688C342FD4D] (laboratory host) C. cephalonica can be mass reared on Jowar along with groundnut, streptomycin, vitamin complex, Na and K salts.&lt;br /&gt;
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[u]Control[/u]: &lt;br /&gt;
Spray Imidacloprid (0.005%; 0.3 ml/L water), acephate 75SP (1.5 g/L water), Etofenprox (0.03%), Phosalone (1.5 ml/L water), Carbaryl (0.15%; 3 g/L water), Monocrotophos (0.04%), Phosphamidon (0.05%) or Methyl Parathion (0.05%). &lt;br /&gt;
* Spray 3 times; First spray should be given during the early stage of flower formation. The second spray should be given at a flower size of 6 to 8 cm, still before blooming. The third spray should be given after fruit setting, when the fruits are the size of a pea. Or:&lt;br /&gt;
* First spray should be given during the early stage of flower formation. The second spray should be given 2 weeks later. Or:&lt;br /&gt;
* Spray 3 times; First spray of imidacloprid (0.005%; 0.3 ml/L water) should be given during the early stage of flower formation. The second spray, thiamethoxam (0.005%; 0.2 g./L water) or acephate 75SP (1.5 g/L water) should be given at after fruit setting. If hopper infestation persists, the third spray, of carbaryl (0.15%; 3 g/L water) should be given prior to fruit maturation.&lt;br /&gt;
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Imidacloprid residues persist in peel for 60 days and in pulp for 50 days. Mature Dashehari fruits at harvest (after 85 days of spraying) were free from imidacloprid residues.[http://www.ncbi.nlm.nih.gov/pubmed/23196371]&lt;br /&gt;
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==Mango Midges==&lt;br /&gt;
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[u]Inflorescence Midge[/u]&lt;br /&gt;
[[Image:Platygaster_sp.jpg|thumb|right| Platygaster sp. [http://liuzhen.000space.com/insect/organism.php?id=1367&amp;amp;type=list]]]&lt;br /&gt;
[[Image:Inflorescence_midge.jpg|thumb|left| [http://www.bitterrootrestoration.com/mango/inflorescence-midge-erosomyia-indica.html Erosomyia indica ©]]]&lt;br /&gt;
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This midge (Erosomyia indica) lays eggs in new inflorescence (preventing flower and fruit set) and new leaves around it, and then the larvae will eat their way through. The maggots penetrate and feed on the tender parts of the plant, such as shoots, buds and flower buds. The flowers will dry and fall off. For pupation, the mature larvae will drop into the soil. The adult midge lives only one day and doesn&amp;#039;t cause any damage. Maggots from eggs laid on new fruits will eat their way into the fruit, which will turn yellow and drop.[http://www.actahort.org/books/231/231_15.htm] The inflorescence midge is an important pest in India in particular.&lt;br /&gt;
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Infloresence midge is parasitized by Platygaster sp., Eupelmus sp. and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19881115612.html;jsessionid=E4CDBFFBEE75C82EDA1BA2CA7B5F1DD2], which all also parasitize Gall Midges. The inflorescence midge is also parasitized by Aprostocetus sp.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9] and Mirufens longifunculata. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false]&lt;br /&gt;
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Another mango midge, Procystiphora mangiferae is parasitized by Pirene sp. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], which also parasitizes mango gall midges.&lt;br /&gt;
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Chemicals: If you have no other option, Parathion, Dimethoate, Phosphamidon and Endosulfan much reduced infestations on mango shoots.[http://www.cabdirect.org/abstracts/19740517480.html;jsessionid=59B14F930CB8EDFD2F1B50F1B04C7692] The most effective insecticide for control appeared to be phosphamidon (Dimecron), whether mixed with diazinon or not, as a foliar spray. [http://www.cabi.org/isc/abstract/19770549886]&lt;br /&gt;
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[u]Gall midges[/u]&lt;br /&gt;
[[Image:Mango_galls.jpg|thumb|left| [http://www.infonet-biovision.org/default/ct/124/crops © A.M. Varela]]]&lt;br /&gt;
[[Image:Eupelmus_urozonus.jpg|thumb|right| Eupelmus urozonus [http://www.biolib.cz/en/image/id150459/ © Miroslav Fiala]]]&lt;br /&gt;
[[Image:Mango_galls_P_matteiana.jpg|thumb|left| P. matteiana galls [http://www.pests.blogfa.com/8907.aspx © RJ Gagné]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Gall_midget_exit_holes.jpg|thumb|left| Exit holes [http://biostor.org/reference/55262 © RJ Gagné]]]&lt;br /&gt;
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There are various mango gall flies:&lt;br /&gt;
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- Erosomyia mangifereae or Procontarinia frugivora has been observed in the Philippines, India, West Indies and Brazil.&lt;br /&gt;
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- Asynapta sp. in West Indies.&lt;br /&gt;
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- Dasyneura mangifereae in Hawaii.&lt;br /&gt;
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- P. allahabadensis, P. biharana, P. brunneigallicola, P. viridigallicola, P. echinogalliperda, P. keshopurensis, P. mangifoliae, P. tenuispatha, P. amraeomyia and Dasyneura amaramanjarae have been observed in India (and some in Pakistan).&lt;br /&gt;
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- P. mangicola in China and Japan.&lt;br /&gt;
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- P. matteiana (1.5 mm long) in India, Kenya, Mauritius, Java and Réunion[http://biostor.org/reference/55262][http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=4&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFEQFjAD&amp;amp;url=http%3A%2F%2Fwww.geocities.ws%2Fmdce2005%2FMDCE2005%2F27-98_102.pdf&amp;amp;ei=HS5zU-P3DomXyQOd_IHgCA&amp;amp;usg=AFQjCNHlZtUS8qYFE2lCVSqh3ow0K4ukLg&amp;amp;sig2=vW-1UFdjzeE3l58kmc8eMg&amp;amp;bvm=bv.66699033,d.bGQ]. &lt;br /&gt;
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The tiny (1-2 mm) mango gall flies lay their eggs on the surface of young leaves, buds, shoots and flowers. After hatching, the maggots will eat their way into the plant tissue. This will show as galls (3-4mm) on the leaves. Once mature, they drop to the ground to pupate. The holes that they left behind are susceptible to fungal infections. Infested fruits initially show small (1mm) brownish lesions, which grow as the fruit grows. In young fruits, the exit holes are usually near the point of attachment, on the bottom side. Most infested fruits fall to the ground before ripening. Optimal conditions for reproduction is 26°C and a relative humidity of 70 to 80%. Infestation particularly occurs at bud-burst stage, at fruit set and on tender leaves of new flushes.[http://openagricola.nal.usda.gov/Record/IND92003672]&lt;br /&gt;
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[u]Natural enemies[/u]; Parasitic insects such as Platygaster sp., Eupelmus sp., Tetrastichus sp., Systasis dasyneurae [http://www.cabdirect.org/abstracts/19881115612.html], Pirene sp. and the predator ants Formica rufa (aka red wood ant, native to Europe and North America), Oecophylla longinoda (weaver ant native to West and Central Africa) and Oecophylla smaragdina (native to South-east Asia) and Camponotus sp. (aka carpenter ant, native to forests world wide).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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[u]Baits[/u]; Coriander oil attracts Erosomyia. D. amaramanjarae is attracted by a mixture of glycine, sodium hydroxide, ammonium carbonate and strong ammonia solution. Bordeaux mixture is a repellent. &lt;br /&gt;
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[u]Chemicals[/u]; Various systemic insecticides are equally effective: Dimecron (phosphamidon), Anthio (formothion) and Metasystox (demeton-S-methyl). Most effective is a mixture of phosphamidon (0.03%) and diazinon (0.03%).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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==Mango Moths==&lt;br /&gt;
[[Image:Indarbela_quadrinotata.jpg|thumb|left| Indarbela quadrinotata [http://www.agriplaza.in/orange-protection.htm#indarbela © Agriplaza]]]&lt;br /&gt;
[[Image:Podagrionella_habitus.jpg|thumb|right| Podagrionella [http://www.waspweb.org/Chalcidoidea/Torymidae/Toryminae/Podagrionini/Podagrionella/index.htm © S. van Noort]]]&lt;br /&gt;
[[Image:Indarbela_quadrinotata_2.jpg|thumb|left| Indarbela quadrinotata [http://www.ku.ac.th/e-magazine/september43/bark_feeders/ © KUAC]]]&lt;br /&gt;
[[Image:Eudocima_materna.jpg|thumb|left| Eudocima materna ]]&lt;br /&gt;
[[Image:Mantis_religiosa_5.jpg|thumb|right| Mantid eating large moth [http://www.agefotostock.com/en/Stock-Images/Rights-Managed/K60-589612 © Oriol Cabrero]]]&lt;br /&gt;
[[Image:Eudocima_materna_2.jpg|thumb|left| Eudocima materna [http://www.indianaturewatch.net/displayimage.php?id=285084 © Sivakumar V K]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Eudocima_homaena_2.jpg|thumb|left| Eudocima homaena [http://gaga.biodiv.tw/9702bx/049.htm © GaGa]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Eudocima_homaena_4.jpg|thumb|left| Eudocima homaena [http://blog.xuite.net/mbbrgs/twblog1/137791571-%E9%91%B2%E8%90%BD%E8%91%89%E5%A4%9C%E8%9B%BE++Eudocima+homaena++(Hubner,+1823)]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-l5.php © PathPiva]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella_2.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-1.php © PathPiva]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Orthaga_exvinacea.jpg|thumb|left| Orthaga exvinacea [http://www.nbaii.res.in/insectpests/images/Orthaga-exvinacea7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Orthaga_euadrusalis.jpg|thumb|left| Orthaga euadrusalis [http://www.jpmoth.org/~dmoth/62_Pyralidae/6003_Epipaschiinae/60031001_Orthaga_euadrusalis_1865/Orthaga%20eudrusalis%200208287801.jpg]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_2.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42845]]]&lt;br /&gt;
[[Image:Aleiodes_indiscretus.jpg|thumb|right| Aleiodes indiscretus [http://commons.wikimedia.org/wiki/File:Aleiodes_indiscretus_wasp_parasitizing_gypsy_moth_caterpillar.jpg © Agricultural Research Service]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42848]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_3.jpg|thumb|left| Citripestis eutraphera [http://www.boldsystems.org/index.php/Taxbrowser_Taxonpage?taxid=375866 © ANIC/BIO]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_2.jpg|thumb|left| P. jocosatrix [http://www.nbaii.res.in/insectpests/images/Penicillaria-jocosatrix7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_3.jpg|thumb|left| P. jocosatrix [http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar © DAFF]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix.jpg|thumb|left| P. jocosatrix [http://www.flickr.com/photos/bettaman/3973171117/ ©]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Caterpillar.jpg|thumb|left| Deanolis sublimbalis [http://www.padil.gov.au/pests-and-diseases/pest/main/136263/43328 © S. Eyres]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Moth.jpg|thumb|left| Deanolis sublimbalis [http://www.ces.csiro.au/aicn/name_s/b_1295.htm © DAFF]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Chlumetia_transversa_2.jpg|thumb|left| C. transversa [http://www.nbaii.res.in/insectpests/Chlumetia-transversa.php © NBAII]]]&lt;br /&gt;
[[Image:Chlumetia_transversa.jpg|thumb|left| C. transversa [http://www.inaturalist.org/observations/572035 © T Bonebrake]]]&lt;br /&gt;
[[Image:Goryphus_basilaris.jpg|thumb|right| Goryphus basilaris [http://www.hkwildlife.net/viewthread.php?tid=49119 © Daydream]]]&lt;br /&gt;
[[Image:Parasa_lepida.jpg|thumb|left| P. lepida [http://fanseab.exblog.jp/9707580]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Parasa_lepida_2.jpg|thumb|left| P. lepida [http://www.isan.clubs.chula.ac.th/para_norkhai/?transaction=post_view.php&amp;amp;cat_main=all&amp;amp;id_main=301&amp;amp;star=270&amp;amp;pg=28 © ปิ่นลม]]]&lt;br /&gt;
[[Image:Parus_major.jpg|thumb|right| Parus major [http://en.wikipedia.org/wiki/Parus_major#mediaviewer/File:Parus_major_2_Luc_Viatour.jpg © Luc Viatour / www.Lucnix.be]]]&lt;br /&gt;
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[u]Bark Eating Caterpillar[/u]&lt;br /&gt;
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The Bark Eating Caterpillar (Indarbela quadrinotata and Indarbela tetraonis) aka Mango Bark Feeder has been observed in India, Bangladesh and Sri Lanka. The moth has a wingspan of maximally 2 cm. This wood boring insect may attack a wide variety of trees, including mango, guava, jujube, pomegranate and apple trees. In plantations, infestation generally starts with the onset of premonsoon rains. The main symptom is plastered dark-brown masses on tree trunks and large branches. These webbed masses consist of chewed on wood remainders / frass, excrements and silken thread. Eggs are laid in clusters on the bark of trees. Initially, the newly hatched larvae occur in groups browsing on the bark. Then they migrate and lodge in shoots and buds. As the larvae grow, they start tunneling into the wood, up to 25 cm deep. This tunnel is kept free of frass. The frass and excreta from the tunnels are used for constructing the extended tunnel mouth (sleeve). As the larvae get bigger, they make the tunnel wider. The larvae hide in these tunnels during the day and come out at night to eat the surrounding bark of the tree. The rate of feeding is faster during the hottest months. The larva minimally eats 16 mg / day, at 12.5 degrees Celsius. The maximum is 166 mg/day at 35 degrees Celsius. Similarly, food consumption is maximum at 96% RH and minimum at 56% RH.&lt;br /&gt;
When large patches of bark are eaten away, the tree gets increasingly exposed to various bacteria, fungi etc. And if substantial areas of bark have been eaten, the tree will eventually die. Prolonged water stress decreases the resistance of the host tree. The larvae pupate inside the tunnel and the emerging moth leaves its &amp;quot;pupal skin&amp;quot; at the mouth of the borer hole. The pupa is 1.5 cm long. The larval stage lasts for about 8 months. The pupal period lasts for about 9 days. It has a little more than one generation per year.  [http://www.tropecol.com/pdf/open/PDF_49_1/09%20Shashidharan.pdf]&lt;br /&gt;
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Natural enemies; Two fungi; Aspergillus candidus and Beauveria bassiana have been reported to cause mortality of the Bark Eating Caterpillar in the field. Laboratory trials have indicated 100% larval mortality by these fungi. The Bark Eating Caterpillar is parasitized by Podagrionella indarbelae (&amp;#039;metallic&amp;#039; parasitic wasps), which may be fed the eggs of I. tetraonis.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf] Podagrionella is found in tropical African countries, Senegal, Algeria, Malawi, France, Spain, India, Thailand, Indonesia, The Philippines, Australia&lt;br /&gt;
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Control; prevent overcrowding by sufficient spacing in between trees. One may insert a sharp metallic probe/spike into each tunnel/hole to kill the larvae. One may seal the tunnel entrance using tar or wax. Of the various insecticides screened against this insect, monocrotophos (0.1 %), quinalphos (0.1 %) and fenvalerate (0.08%) gave best results.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf]&lt;br /&gt;
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[u]Fruit-piercing Moths[/u] &lt;br /&gt;
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Eudocima (aka Othreis) are very large moths (up to 10 cm wingspan) with orange abdomen. The most widespread specie is [http://www.waiwiki.org/index.php?title=Eudocima_phalonia Eudocima fullonia] (aka Eudocima phalonia)[http://www.hindawi.com/journals/tswj/2011/753484/] Eudocima moths look like dead leaves when in hiding mode. Until they show their bright orange hindwings with broad black margins and a large black spot in the middle. Mature larvae are 4 to 5 cm long. These moths have been observed in virtually any tropical country, except for the Americas. The adults fly (very well), feed and mate during the night. In tropical countries, they live up to a month. The adults feed on fruit juice by puncturing the fruit. The resulting wound is used by various bugs and diseases to attack the fruit (including mango, banana, grapes, orange, clementine, kiwi, lychee, pineapple, peach, apricot, papaya apple and pear). Eggs are laid singly, or in masses (up to 750 in a cluster, which may be used for rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings]), on any part of the plant. In tropical conditions, eggs hatch within 4 days. Larvae may feed around the clock and mature in 3 weeks, after which they pupate in cocoons of silk-spun leaves. Pupation favors wet conditions and occurs in 12 to 18 days.[http://www.aphis.usda.gov/plant_health/plant_pest_info/pest_detection/downloads/pra/efulloniapra.pdf] Pupation is always in a roomy cell made of living leaves woven together with silk and slightly lined; the pupa is attached to this lining by the cremaster.[http://www.mothsofborneo.com/part-15-16/calpini/calpini_4.php] Temperatures below 16°C during the activity period after dusk prevents feeding, mating and oviposition.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] &lt;br /&gt;
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Their larvae do not feed on mangoes, nor the mango trees. Instead, the larvae feed on very specific Fabaceae and Menispermaceae. Variation in the alkaloids associated with certain menisperm genera (eg quaternary alkaloids in Tinospora[https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2007-969933]) may explain the very specific (or even exclusive) moth–host plant relationships.[http://www.publish.csiro.au/?paper=SB9960227] Eudocima larvae are known to feed extensively on Stephania japonica (Menispermaceae) and its varieties.[http://www.calodema.com/freefiles/412.pdf] In Australia the larvae of Eudocima salaminia feed almost exclusively on the forest vine, Stephania japonica.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] &lt;br /&gt;
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Eudocima materna feeds exclusively on the genus [http://www.waiwiki.org/index.php?title=Eudocima_phalonia#Tinospora Tinospora].[http://onlinelibrary.wiley.com/doi/10.1111/j.1442-9993.1993.tb00471.x/abstract] (widespread in tropical regions [http://link.springer.com/article/10.1007/s00606-004-0293-1#page-1], including Africa [http://informahealthcare.com/doi/abs/10.1080/14756360802333075]). &lt;br /&gt;
Eudocima materna occurs in Ghana (spotted at Vane and Sokode-Etoe, close to Ho in Volta region), Ethiopia, Brazil, Ecuador [http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=140] Nigeria, Pakistan, India, Australia, Costa Rica [http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=40][http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=20][http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=00] and Mexico [http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=120]. Eudocima materna and fullonia moths attack citrus and mango [http://www.cabdirect.org/abstracts/19370500524.html], but particularly tomato.[http://www.aapmhe.in/index.php/pmhe/article/view/42]&lt;br /&gt;
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Eudocima homaena may feed on Quisqualis indica.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41], Achyranthes, Cocculus, Cyclea, Menispermum and Tiliacora species.[http://www.en.inforapid.org/index/index.php?search=Cocculus] Eudocima jordani and Eudocima fullonia may be generalist feeders.[http://www.researchgate.net/publication/249439696_Differential_habitat_affinities_of_five_species_of_fruitpiercing_moths_(Lepidoptera_Noctuidae)_in_their_utilization_of_Tinospora_smilacina_Benth._as_a_larval_host_plant_in_north_Queensland]&lt;br /&gt;
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Control; Regularly check the orchard during the night using a flashlight. The adult Eudocima moths have large eyes that glow red when illuminated. Adult [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] may eat large moths such as Fruit-piercing moths. The female Praying mantis lays a few hundred eggs in a cluster (ootheca) attached to an object like a branch or trunk. Large species of mantids may have a length of up to 12 cm. Their colour is usually adapted to the environment they naturally live in. They have about 2 generations per year. Young mantids look like adults, but don&amp;#039;t have wings. Adult females often have no wings either, or reduced wings.&lt;br /&gt;
Eudocima are parasitized by Euplectrus [http://link.springer.com/article/10.1023/B:BICO.0000036439.74117.2f#page-1][http://www.aapmhe.in/index.php/pmhe/article/view/42] and Trichogramma.[http://images.peabody.yale.edu/lepsoc/jls/2010s/2011/2011-65-1-053.pdf]&lt;br /&gt;
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[u]Honeydew Moth[/u]&lt;br /&gt;
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The Honeydew Moth (Cryptoblabes gnidiella aka Albinia casazzar aka Albinia wockiana) aka Christmasberry Moth survives only in warm climates (all over the world). It attacks a large variety of crops, including mangoes, banana, oranges, clementines, grapes, loquats, pomegranates, avocado, coffee, maize and rice. The caterpillars (1 cm long) mainly feed superficially on fruits, but may also feed on leaves, flower, shoots and bark. The newly hatched larvae and the adults, however, solely feed on honeydew. The adults are active during the night. Females mate 1 to 4 times during their entire life. One Honeydew Moth may lay up to 100 eggs within 2 days after mating, on fruit or leaves (averagely 150 in total, during lifetime). These eggs hatch within one week (or longer, in colder regions). It has several generations per year, depending on the climate. Adult moths are good flyers (1 to 2 cm wingspan) and may rapidly spread to poorly maintained orchards (infested with honeydew).&lt;br /&gt;
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Natural enemies; Scolothrips sexmaculatus and Orius spp. prey on the eggs and early-instar larvae. The Honeydew Moth is parasitized by Phanerotoma sp.[http://www.cabdirect.org/abstracts/19740514736.html] and Trichogramma Platneri (both parasitoid wasps).&lt;br /&gt;
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Trichogramma are extremely tiny wasps (&amp;lt; 0.5 mm). They insert their eggs into the eggs of the moth. The larvae feed, grow and pupate inside the host egg, which converts the colour of the host egg from white to black. The emerging wasp eats its way out and is ready to mate. The eggs from unmated females will produce only males, whereas the eggs from mated females will produce both males and females. Adult Trichogramma feed on nectar from flowers. One female wasp may parasitize 50 moth eggs during her life. The optimum conditions for Trichogramma are 20 to 27 degrees Celsius and 60% RH. Most insecticides kill Trichogramma.&lt;br /&gt;
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Control; This moth is attracted by honeydew secreted by aphids, scale insects and other honeydew secreting bugs. It is therefore most effectively controlled by controlling all honeydew secreting bugs. Its instars are highly susceptible to Bacillus thuringiensis.&lt;br /&gt;
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[u]Leaf Webber[/u]&lt;br /&gt;
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These brown moths (Orthaga euadrusalis and Orthaga exvinacea) are a minor mango (and Cashew nut) pest. They have been observed in India and Indonesia. Females mate only one time during their whole life.[http://www.ncbi.nlm.nih.gov/pubmed/20136014] The leaf webber lays eggs on young leaves and buds and creates a web around pods, leaves and flowers for its larvae to pupate in. Such a webbed cluster may contain several larvae. The Leaf Webber causes relatively very little damage, as the larvae merely scrape and eat from the surface of the leaves.&lt;br /&gt;
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Control: Application of Serratia marcescens (a bacterium), Aspergillus flavus (a fungus) and Beauveria bassiana (a fungus) are all very effective.[http://www.cabdirect.org/abstracts/19810586732.html] The leaf webber is parasitized by Brachymeria lasus, Hormius sp., Pediobius bruchicida and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html] and also by Trichogramma chilonis and Trichogramma pretiosum.[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=37933]&lt;br /&gt;
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[u]Mango Fruit Borer[/u];&lt;br /&gt;
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The Mango fruit borer (Citripestis eutraphera; a moth) has been observed in Indonesia, India and Australia. Its lays its eggs (&amp;lt; 1 mm) on fruit or stalk. The larvae feed on mango pulp (and cashews) and cause premature fruit drop, mainly in young fruit. The larvae hatch in 2 days. They are initially white, then red-violet when young, turning to dark blue as they grow. Hatched larvae initially feed on the peel of the fruit. Then they bore into into the fruit and feed for 12 to 15 days before they pupate in the fallen fruit, or in the soil. Often there is more than one larva in a fruit with a total of 15 recorded in one fruit.[http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42846].&lt;br /&gt;
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Control: Its natural enemies are parasitoid wasps (Aleiodes sp. and Euplectrus sp.) and a parasitoid fly (Blepharella lateralis).&lt;br /&gt;
[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=9416] To protect the stems, cover them with a cloth or Jute and paste charcoal over it. Fostoxin tablets can also be placed and sealed in the holes made by the borers.&lt;br /&gt;
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[u]Mango Shoot Borer[/u]&lt;br /&gt;
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Mango Shoot Borer (Penicillaria jocosatrix) or Velvet mango slug; P. jocosatrix (wingspan 2 - 2.5 cm) has been observed in Indonesia, The Philippines, Malaysia, Vietnam, Guam, Hawaii, Thailand and Australia. Its larvae; the caterpillar of this moth (up to 3 cm long) causes damage to shoots, stems, inflorescence, flowers and fruits of the mango tree. Pestalotiopsis anacardiacearum sp. nov. (fungal leaf rot) is found on dead mango leaves associated with P. jocosatrix (the Mango Shoot Borer).[http://biotaxa.org/Phytotaxa/article/view/phytotaxa.99.2.1] Eggs hatch in 3-5 days. Larval development takes 8-10 days. Mature larvae pupate in the soil and the moth emerges 16-20 days later.[http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar]&lt;br /&gt;
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Natural enemies: Euplectrus sp. (a parasitoid wasp) and Blepharella lateralis (a parasitoid fly) may effectively decrease Mango shoot borer infestation [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], and are also effective against the Mango fruit borer.&lt;br /&gt;
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[u]Mango Seed Borer[/u]&lt;br /&gt;
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The Mango Seed Borer (Deanolis sublimbalis aka Autocharis albizonalis) is the Red banded mango caterpillar. It is a mango pest in Australia, Burma, The Philippines, Malaysia, Vietnam, China, Indonesia and Papua New Guinea. Severe infestation may cause up to 50% yield reduction. This grey snout moth (wingspan 2 cm) lays its eggs on the top of the mango. Its larvae (one or more per fruit) develop within the fruit in 2 to 3 weeks. Initially they feed on the pulp, and then on the seed. The fruit will split, rot and drop. The mature larvae will pupate in the soil.&lt;br /&gt;
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Natural enemies: Rychium attrisimum are wasps that feed on the larvae of the Mango Seed Borer as they leave the fruit.[http://www.planthealthaustralia.com.au/wp-content/uploads/2013/03/Red-banded-mango-caterpillar-CP.pdf] Trichogramma chilonis and Trichogramma chilotreae are tiny parasitoid wasps that parasitize the eggs of the Mango Seed Borer and hundreds of other moths. They are so small that with the naked eye you cannot see what they are. They have a wingspan of 0.5 mm. They live about 2 weeks, and may lay several eggs in a single moth egg.&lt;br /&gt;
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Chemicals: If you have no other option, Deltamethrin and Cyfluthrin are most effective.&lt;br /&gt;
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[u]Mango Tip Borer[/u]&lt;br /&gt;
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Mango Tip / Shoot / Bark / Trunk / Twig Borer (Chlumetia transversa) aka aka Shoot Boring Caterpillar is a serious mango (and litchi) pest. The major symptom is drying of the tip of young shoots. Female moths lay their eggs on tender leaves. Newly hatched larvae bore into these leaves. As they grow they bore into young shoots near the growing point, and subsequently tunnel down. The leaves of affected shoots will droop. It has four generations each year, and in colder regions overwinters as pupae in young branches and bark. Generations are overlapped.[http://europepmc.org/abstract/CBA/367089] Full grown caterpillars are 2 to 2.5 cm long.&lt;br /&gt;
C. transversa has been observed in Indonesia, The Philippines, Malaysia, Thailand, Taiwan, China, India, Pakistan, Sri Lanka and Australia. &lt;br /&gt;
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The Mango Tip Borer is parasitised by Bracon greeni, Meteorus sp. and Goryphus sp [http://www.cabdirect.org/abstracts/19810586735.html], which are all parasitoid wasps. Its larvae are also parasitized by the fly Megaselia chlumetiae sp. nov. Its larvae enter the host and develop by consuming the host&amp;#039;s internal tissues. Pupariation normally takes place within the host&amp;#039;s empty skin.[http://www.cabdirect.org/abstracts/19921163681.html]&lt;br /&gt;
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Control; If you have no other option, spraying 2.5% deltamethrin EC for three times may be most effective (87% control efficiency) and the least harmful to the tree. Four insecticides tested; 80% dichlorvos EC(dilution of 1250), 47.5% chlorpyrifos EC(dilution of 1000), 2.5% deltamethrin EC (dilution of 2500) and 2% abamectin EC(dilution of 2000) were applied on mango trees during 2 months. Second best was 2% abamectin EC at a dilution of 2000. The other two insecticides were less effective, which might be due to the development of resistance in pests against these insecticides on account of longer repeated applications.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GXNY200910010.htm]&lt;br /&gt;
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[u]Nettle Caterpillar[/u]&lt;br /&gt;
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The Nettle Caterpillar, aka the Blue-striped Nettle Grub (Parasa lepida aka Latoia lepida; 2 to 3 cm long) has been observed in China, Pakistan, India, Sri Lanka, Bangladesh, Vietnam, Thailand, Malaysia, Japan and especially Indonesia. Parasa lepida may infest various fruit trees, including mango, cherry, persimmon, Indian almond and rose apple, and also coffee, tea, cocoa and palms, with a preference for coconut trees. The caterpillar of this species have spines containing a poison that causes serious irritation and inflammation in humans upon contact. It feeds on the leaves and shoots of the mango tree. Initially, defoliation will be localized, affecting only one or a few trees. Thus the infestation is easily identified. Subsequently, as new generations of this pest emerge, the infested area may rapidly expand. The female moth lays eggs on mango leaves. The cocoons are laid side by side. The cocoons are so hard and stout that they remain on the trunks for 5 to 6 years after spinning. Cocoons from which adults have successfully emerged have clear emergence holes. Newly hatched caterpillars will feed on the underside of the epidermis, stripping it off the leaflets. They often begin where the eggs were laid; at the tip. Then they eat the edges of the leaflet and eat large areas of the lamina. When they have finished developing, the whole leaflet will have been consumed systematically from tip to base, leaving only the midrib. On this midrib, the notched indentations are the only visible remainders left by the caterpillars. [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=38935]&lt;br /&gt;
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Parasa lepida is predated on by Agriosphodrus dohrni (The Assassin Bug, native to China, India, Indonesia). This 2 cm long Assasin Bug may be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
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The great tit (Parus major) is a predator of Parasa lepida cocoons in Japan. The most effective parasites are Apanteles parasae (a parasitoid wasp) and Chaetexorista javana (a parasitoid fly).[http://www.cabdirect.org/abstracts/19820596771.html]&lt;br /&gt;
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Salt spray from the sea and the resulting plant stress (considerable damage) may negatively affect the Parasa lepida moth.[http://www.bioone.org/doi/pdf/10.3956/0031-0603-83.3.193]&lt;br /&gt;
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==Mango Scales==&lt;br /&gt;
[[Image:Coccus_viridis.jpg|thumb|left| Coccus viridis [http://www.insectimages.org/browse/detail.cfm?imgnum=5385208 © J.W. Lotz]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| M. signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
[[Image:Coccus_hesperidum.jpg|thumb|left| Coccus hesperidum [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/09Cocc_hesperidum/4hesperidumHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Ceroplastes_floridensis.jpg|thumb|left| Ceroplastes floridensis [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/03Cero_floridensis/4floridensisHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Chilocorus_kuwanae.jpg|thumb|right| C. kuwanae [http://animal.memozee.com/view.php?tid=2&amp;amp;did=10472 © Jinsuk Kim]]]&lt;br /&gt;
[[Image:Aulacaspis_tubercularis.jpg|thumb|left| Aulacaspis tubercularis[http://gaga.biodiv.tw/new23/9409/076.htm]]]&lt;br /&gt;
[[Image:Azya_orbigera.jpg|thumb|right| A. orbigera [http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php © E Arcaya]]]&lt;br /&gt;
[[Image:Drosicha_mangiferae.jpg|thumb|left| D. mangiferae [http://www.jugantor.com/last-page/2014/04/19/89802 ©]]]&lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri.jpg|thumb|right| C. montrouzieri[http://www.ozanimals.com/Insect/Mealybug-Ladybird/Cryptolaemus/montrouzieri.html ©]]]&lt;br /&gt;
[[Image:Rastrococcus_iceryoides.jpg|thumb|left| R. iceryoides [http://www.nbaii.res.in/insectpests/Rastrococcus-iceryoides.php © NBAII]]] &lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri_larva.jpg|thumb|right| C. montrouzieri larva [http://www.insectimages.org/ © J.W. Lotz, FDACS Bugwood.org]]]&lt;br /&gt;
[[Image:Paracoccus_marginatus.jpg|thumb|left| Papaya mealybug [http://www.ablturismo.com/avispa-enviada-por-correo-salva-cultivos-en-la-india/ ©]]]&lt;br /&gt;
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The main mango scales are:&lt;br /&gt;
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* Coccids ; Coccus viridus (soft green scales) and Coccus hesperidum (soft brown scales)&lt;br /&gt;
* wax scales ; Ceroplastes spp.&lt;br /&gt;
* armoured scales ; Aulacaspis tubercularis, Chloropulvinaria polygonata (the Mango Green Shield Scale) and Aspidiotus destructor.&lt;br /&gt;
* Mealybugs (pseudococcidae) ; Drosicha mangiferae, Rastrococcus iceryoides (&amp;quot;Mango mealybugs&amp;quot;) and Paracoccus marginatus (&amp;quot;Papaya mealybug&amp;quot;, which is also a mango pest)&lt;br /&gt;
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Mango scales suck the sap from the leaves, and as a result, the leaves start drying. This may cause branches to die, blemished fruits and premature dropping. Scales are small insects (1 to 7 mm), generally immobile; as if shells are glued to the plant. Only the newly hatched crawlers (emerging from under a big scale) move to their feeding site. Soft scales produce honeydew, which may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould]. Armoured scales don&amp;#039;t excrete honeydew. The papaya mealybug has been observed in Mexico, Florida, Antigua, Cuba, Dominican Republic, Guadeloupe, Haiti, St Kitts, Martinique, Puerto Rico, St Martin, St Barthélémy, Virgin Islands.[http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=6&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEIQFjAF&amp;amp;url=http%3A%2F%2Fwww.cabi.org%2FISC%2FFullTextPDF%2F2013%2F20133231104.pdf&amp;amp;ei=VeZ8U_eFNaqX1AXh_YHYDA&amp;amp;usg=AFQjCNGWK-GGi3IRhAfSNBg6nah3XhoKWw&amp;amp;bvm=bv.67229260,d.d2k]&lt;br /&gt;
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[u]Natural enemies[/u]; Natural enemies of scales include parasitic wasps, ladybird beetles and lacewings, which are killed by broad-spectrum pesticides. Because of their honeydew secretion, scales may be protected / farmed by specific ants (eg arboreal ants (Azteca instabilis) farming soft green scales).[http://www.ncbi.nlm.nih.gov/pubmed/18559179] Some fungal pathogens effect this symbiotic interaction.[http://www.ncbi.nlm.nih.gov/pubmed/23905728] Parasitoid insects use the honeydew secreted by coccids (and aphids etc) as an infochemical to locate their hosts.[http://www.ncbi.nlm.nih.gov/pubmed/15112724] R. iceryoides is parasitized by Dinocarsis sp., Microterys flavus, Metastenus concinnus and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
Of the parasitoids Acerophagus papayae and Anagyrus loecki, A. papayae is the most effective in decimating the papaya mealybug. (Meyerdirk)&lt;br /&gt;
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[u]Lacewings[/u]; Most lacewings (also) feed on plant material, such as pollen. Particularly green lacewings such as Mallada boninensis, Mallada astur and Mallada signata are mainly predatory and very effective. They are relatively small (length up to 1.5 cm, including wings). Adults are active during the night, and may be attracted by flashlight. Eggs are attached to leaves and hatch within 4 days. The larval period lasts 12 days, and the pupal period lasts about 9 days. Adults live just one month. One may commercially rear millions of green lacewings for biological control of mango pests.&lt;br /&gt;
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[u]Cryptolaemus montrouzieri[/u] (aka Mealybug Ladybird) is a (4 mm long) predatory ladybird that eats mealybugs, soft scales (Coccidae) and armoured scales (Diaspididae). Originally from Australia, introduced in Florida and commercially available as a mealybug control agent. It needs warm, humid weather. Survival temperature range is 15-35°C. Life cycle on average is 30 days under optimum conditions: 23 °C and 60-70% humidity. Optimal temperature for adults is 28°C. They are active between 16°C and 33°C, and 70 to 80% RH. Longevity of females is 70 days max. It produces 4 generations per year. Development is accelerated at higher temperatures. At 25°C it may lay a total of 200 to 700 eggs (av. 400), at the rate of averagely 9 eggs per day. They preferrably lay their eggs in the egg mass of mealybugs. The eggs hatch in 5 to 6 days. There are 4 larval stages, and the entire larval period lasts about 12 to 17 days. At pupation stage they are about 13 mm long. Adults emerge after 7 to 10 days. These beetles prefer high concentrations of mealybugs to predate on, and will fly away when concentrations of mealybugs are lower.[http://www.arabscientist.org/english/page/11/] During its larval development, one C. montrouzieri may eat 2400 eggs of C. polygonata.[http://www.cabdirect.org/abstracts/19981112241.html] Unfortunately, the filaments produced by its larvae look very similar to mealybugs. Initially release 1 or 2 new active beetles per square meter in the morning, before it gets hot. Subsequently regularly release 2 to 3 beetles per tree. The beetles will be killed by pesticides, but tolerate copper-fungicides.&lt;br /&gt;
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[u]Chilocorus kuwanae[/u]; This ladybird beetle is a primary predator of wax scales (Ceroplastes sp.). This ladybird beetle is attracted by (spraying) methyl jasmonate, mainly due to the terpenoid compound alpha-pinene [http://www.ncbi.nlm.nih.gov/pubmed/19825299]&lt;br /&gt;
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[u]Microweisea coccidivora[/u]; a tiny (1mm long) predatory ladybird beetle from Florida and South Carolina (USA) that feeds particularly on armoured scales.&lt;br /&gt;
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[u]Azya orbigera[/u]; The larvae of this ladybird beetle predate on Coccus viridis. These larvae are relatively immune to ant attacks (ants that farm coccids), due to their sticky waxy filaments. Also, the presence of ants reduces A. orbigera larvae getting parasitized.[http://www.ncbi.nlm.nih.gov/pubmed/18348805] A. orbigera has been observed in Colombia, Guyana, Venezuela, Bélize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, México, USA, Trinidad and the West Indies.[http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php]&lt;br /&gt;
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[u]Sumnius cardoni[/u] (a predatory ladybird) predates on nymphs of D. mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19810586735.html] &lt;br /&gt;
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[u]Coccodiplosis sp[/u] (a parasitic gall midge)[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9], Cybocephalus sp. (a predatory beetle) and Scymnus coccivora (a predatory ladybird) predate on R. iceryoides (mealybug). &lt;br /&gt;
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[u]Cryptochetum sp.[/u] (parasitic flies) parasitize the 1st- and 2nd-instar nymphs of the Drosicha mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
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[u]Coccophagus scutellaris[/u] parasitizes Coccus hesperidum by laying eggs in its gut.[http://www.ncbi.nlm.nih.gov/pubmed/747455] When parasited and parasiteless hosts are available, the female of Coccophagus deposits more eggs on the latter.[http://www.ncbi.nlm.nih.gov/pubmed/7283545]&lt;br /&gt;
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[u]Thomsonisca pakistanensis[/u] is a parasitic wasp that most commonly parasitizes Aspidiotus destructor. A. destructor is also parasitized by Aneristus ceroplastae (tiny parasitic wasp), Comperiella bifasciata (parasitic wasp also effective against yellow scales; Aonidiella citrina[http://www.publish.csiro.au/paper/ZO9710053.htm]), Chartocerus sp. and Chrysonotomyia sp. (both also parasitic wasps) [http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
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[u]Aphytis sp[/u]; This tiny (2 to 3 mm long) parasitic wasp parasitizes Aulacaspis tubercularis (up to 46% parasitism in a test).[http://www.actahort.org/books/509/509_96.htm]&lt;br /&gt;
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[u]Lecanicillium lecanii[/u]; This fungus kills various coccids, including Coccus hesperidum.[http://www.ncbi.nlm.nih.gov/pubmed/20863711] Also Ceroplastes sp. may be killed by Lecanicillium lecanii. Adding body materials of life coccids to a multi-generation culture (medium), significantly keeps the vigor and higher virulence of this fungus.[http://www.ncbi.nlm.nih.gov/pubmed/20387464]&lt;br /&gt;
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[u]Control[/u]: Collect the affected leaves and burn them. Spraying chlorogenic acid (a phenol naturally present in coffee beans) stimulates locomotory activity of the green scale Coccus viridis, thus minimizing their feeding. [http://www.ncbi.nlm.nih.gov/pubmed/20857759]&lt;br /&gt;
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If you have no other option: Use Metasystox 25% EC at the rate of 0.3 L in 450 L water or Fotidal 50 EC at the rate of 0.5 L in 450 L water / acre. Or spray mineral oil at low concentrations (not damaging the trees) after fruit picking, but not during flowering. Don&amp;#039;t spray during droughts or excessive heat.&lt;br /&gt;
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==Weevils==&lt;br /&gt;
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[u]The Mango Stone Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_mangiferae.jpg|thumb|left| Mango Stone Weevil [http://en.wikipedia.org/wiki/Sternochetus_mangiferae#mediaviewer/File:Mango_seed_weevil_2.jpg © P.Jeganathan]]]&lt;br /&gt;
[[Image:Oecophylla_smaragdina.jpg|thumb|right| Stone Weevil captured by O. smaragdina [http://www.cabi.org/isc/datasheet/16434 © Renkang Peng]]]&lt;br /&gt;
[[Image:Mantis_religiosa.jpg|thumb|right| Praying mantis [http://www.statesymbolsusa.org/Connecticut/insect_praying_mantis.html © Andy Williams / CritterZone]]]&lt;br /&gt;
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The Stone Weevil (Sternochetus/Curculio/Rhynchaenus/Cryptorhynchus mangiferae) aka Mango Seed / Nut Weevil is a short weevil; 7 mm to 1 cm long and 4 mm wide. It possesses a tough exoskeleton. Weevils are a type of beetle. The scales of the Stone Weevil may be of various colours; shades of black, grey, red or yellow. The Stone Weevil has been observed in many mango-producing countries all over the world (including Ghana, Nigeria, Kenya, Mozambique, Tanzania, Uganda, Zambia, India, Bangladesh, Indonesia, Thailand, Malaysia, Australia, USA)[http://www.cabi.org/isc/datasheet/16434]. Adult weevils can hardly fly, and this pest spreads by fruit transports. At dusk and during the night, the adults feed on leaves and young shoots. The female lays her eggs on and just under the surface of the peel of young (about 3 cm long) mangoes. Each female can lay up to 15 eggs per day and may lay several eggs on one fruit. The Stone Weevil prefers the sweetest (high-sugar) varieties of mango. After laying an egg, the weevil will make an incision in the fruit to let the sap from the fruit cover the egg, and to make it stick to the fruit. The amber-coloured sap will harden out, and remain as a protective resin mark over the egg. Newly emerged grubs are white and slender, and have no legs. They bore their way through the pulp towards the seed, within 2 days. Larvae and pupae develop inside the fruit. Pupation occurs in the seed. No external symptoms of attack are readily visible on infested fruits. Once matured (which takes up to 2 months), they eat their way out. About 25% of adult the weevils over-winter in the seed. They can live 2 years. One generation is produced each year.&lt;br /&gt;
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Natural enemies; The Stone Weevil is effectively predated on by Weaver ants such as Oecophylla smaragdina and Oecophylla longinoda.[http://iiste.org/Journals/index.php/JBAH/article/view/12284] Weaver ants, however, may also farm (for the produced honeydew) and protect aphids and various mango scales against their natural enemies. &lt;br /&gt;
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Weevils are also predated on by [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids], which may eat anything they can catch, including flies, beetles and moths. Adults readily mate in captivity and have one generation per season. Rearing mantids requires rearing of other insects, such as aphids and drosophilae (for nymphs), tephritids (for young mantids) and moths (for adult mantids) in large quantities. Females lay their eggs in a sticky cluster of several cm long. Nymphs (very tiny praying mantids) emerge from eggs. The developing nymphs need to be separated and fed aphids/drosophilae, or they will become cannibalistic.&lt;br /&gt;
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Control; Collect and destroy all fallen fruits, seeds, leaves, twigs etc. If you have no other option, spraying Fenthion (0.01%) on the trees at the time of oviposition is found to be effective. Fenthion, however, is extremely toxic to beneficial insects [http://www.actahort.org/books/509/509_96.htm], so that applying Fenthion may lead to secondary infestation of Mealybugs. A single spray of tiametoksam (Actara™ SC 240g/ℓ a.i.) at the roots during flower set may be effective for seasonal control.[http://etd.uovs.ac.za/ETD-db/theses/available/etd-09182009-083002/unrestricted/LouwCE.pdf] In Alphonso and Bagan Pali mangoes, a single spray (at dusk) of Monocrotophos 36EC 1ml per 1 litre of water was found to be very effective (97.5% to 100% mortality) [http://issuu.com/kisanadmin/docs/mango1/16] In a laboratory test, Beauveria bassiana was somewhat effective (30% mortality in 14 days), but in an orchard setting there was no effect at all.[http://www.cabdirect.org/abstracts/19971101160.html]&lt;br /&gt;
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[u]The Mango Pulp Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_frigidus.jpg|thumb|left| Mango Pulp Weevil [http://www.thaibugs.com/?page_id=284 © Thaibugs]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
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The Mango Pulp Weevil (Sternochetus frigidus or Cryptorrhynchus gravis) has been observed in Bangladesh, India, Indonesia, Malaysia, Myanmar,&lt;br /&gt;
Pakistan, Papua New Guinea, Philippines, Singapore and Thailand. This female weevil lays eggs (25-60 eggs / week) on mango fruits (minimum 6 cm diameter). Newly hatched larvae tunnel into the fruit pulp. The larvae form a chamber (constructed of frass) next to the seed, from which they eat their way through the pulp. Pupation occurs within these chambers. Matured weevils leave the ripe fruit through an exit hole in the peel. Prior to this exit, nothing shows that the fruit is infected. They are fully matured after 6 weeks; able to mate, repeatedly. One complete life cycle varies from 34-35 days. More than half of adult weevils hibernate in seeds. Mango Pulp Weevils are very poor flyers (maximum 90 cm horizontally).[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Thrips==&lt;br /&gt;
[[Image:Frankliniella_occidentalis.jpg|thumb|left| F. occidentalis [http://conabio.inaturalist.org/taxa/243761-Frankliniella © Th0th]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Thrips, or thunderflies, thunderbugs, storm flies, thunderblights, storm bugs, corn flies or corn lice may feed on many plants. Frankliniella bispinosa, Frankliniella kelliae (Florida, Caribbeans) and Frankliniella occidentalis (California, Israel) also feed on mango. They feed on young leaves, shoots and flowers, causing discoloration and deformation.&lt;br /&gt;
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Thrips are tiny (1 mm long on average; varying from 0.5 mm to 1.4 cm). They are not good flyers, but may readily be carried by the wind. Given the right conditions, they may rapidly multiply and form large swarms. To inspect trees for thrips infestation, just hold a bucket and shake a branch, and some thrips will fall off the infested branch into the bucket.&lt;br /&gt;
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Control: Natural enemies of thrips are Green lacewings (eg Mallada boninensis, Mallada signata), Orius insidiosus, Anystis agilis and Hypoaspis aculifer. Biological insecticides such as Beauveria bassiana and Verticillium lecanii may be effective.&lt;br /&gt;
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[u]Orius insidiosus[/u] is a 3 mm long pirate bug (aka insidious flower bug). They feed on thrips, mites, small caterpillars and various insect (including aphids&amp;#039;) eggs and larvae, and also pollen. They prefer eating thrips over aphids.[http://www.ncbi.nlm.nih.gov/pubmed/18845007] Orius may kill several thrips per day, beyond their need for nutrients. Adults fly well, and may readily disperse, looking for prey. O. insidiosus may be mass reared on [http://www.waiwiki.org/index.php?title=Armyworm armyworms]. They may occasionally sting people, which may hurt, but is completely harmless. Eggs are laid and embedded in the plant tissue of the fruit, stem, leave stalks and big veins at the underside of leaves. From eggs to adults, Orius goes through 5 larval stages. This takes about 16 to 18 days at 25°C. They are predatory at each stage (except as pupae). Adults live 3 to 4 weeks. As Orius albidipennis lays more eggs and has a shorter lifecycle, it may be a better biocontrol agent than Orius insidiosus.[http://eurekamag.com/research/030/621/030621437.php] Release at 2 to 3 Orius per square meter.&lt;br /&gt;
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==Whiteflies==&lt;br /&gt;
[[Image:Trialeurodes_vaporariorum.jpg|thumb|left| Trialeurodes vaporariorum [http://en.wikipedia.org/wiki/File:Weisse-Fliege.jpg © Gaucho]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| Orius insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Paraleyrodes_bondar.jpg|thumb|left| Paraleyrodes bondar [http://manateeornprod.wordpress.com/2012/02/22/new-whitefly-in-florida/ © UF/IFAS]]]&lt;br /&gt;
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Whiteflies (incl. Aleurodicus rugioperculatus, Aleurodicus dispersus, Aleurothrixus floccosus, Aleyrodes proletella, Bemisia tabaci, Paraleyrodes bondar, Siphoninus phillyreae, Trialeurodes vaporariorum) attack various crops, including mango, orange, tomato and watermelon. &lt;br /&gt;
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Whiteflies suck the saps from leaves, causing direct damage. They are also vectors of various diseases, including viruses. Additionally they may produce a lot of honeydew, which may lead to sooty mould. The density of the whitefly is positively correlated with maximum temperature and negatively correlated with relative humidity.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Natural enemies[/u]; Various animals predate on whiteflies, including green lacewings, Orius insidiosus and ladybird beetles. The minute parasitic wasps Encarsia guadeloupae and Encarsia haitiensi very effectively cause reduction in the population of Aleurodicus despersus.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Control[/u]&lt;br /&gt;
Dissolve 1 kg of sugar per 1 L of water. Poor in plastic transparent bottle. Drill holes in the upper part (above water level); small enough for whiteflies to enter, but not for bees. Put the lid on. Hang 2 to 3 bottles in each tree at flower set. Renew weekly.&lt;br /&gt;
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=Integrated Pest Management=&lt;br /&gt;
Integrated Pest Management (IPM) integrates proper orchard managment (such as sufficient spacing of trees, pruning off overlapping branches, the use of bait traps and removing affected fruits and plant parts) and the use of a variety of anti-bugs (natural enemies of mango bugs), so that they are complementary and sufficiently reduce all pest populations, minimizing the use of pesticides.&lt;br /&gt;
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Below is an example of the complementary use of anti-bugs, for both predation and parasitism.&lt;br /&gt;
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Preliminary requirements: A sealed, ventilated room. Constant supply of mangoes, or grapes, kiwi, lychee, pineapple, peach, apricot, papaya, apple or pear, to feed and rear Drosophilae (2-4mm long &amp;#039;fruit flies&amp;#039;), Tephritids (4-7 mm long &amp;#039;true&amp;#039; fruit flies) and Eudocima sp. (fruit piercing moths; up to 10 cm wingspan). One section needs daylight exposure, for growing Fabacae / Menispermacae for the Eudocima sp. to lay their eggs, and for their larvae to feed on the leaves. This sealed room should rather be too large than too small to prevent a lack of food for the reared predators. Any surplus of eggs and/or larvae may be sold as fish feed.&lt;br /&gt;
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* Rearing [http://www.waiwiki.org/index.php?title=Praying_mantis Praying Mantids] to predate on moths, caterpillars, weevils and young Longicorn beetles by adults, and on fruit flies, hoppers and midgets by young mantids. These mantids may be reared on fruit flies  (Drosophila and Tephretids) and specific moths. Young mantids should be separated after hatching, in boxes. Nymphs should be fed Drosophilae. Young mantids should be fed Tephretids. Adult mantids are fed Eudocima moths or Armyworm moths.&lt;br /&gt;
* Rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings] (Mallada signata, Mallada boninensis) to predate on aphids, scales, hoppers and thrips in the field. These lacewings may be reared on Eudocima sp. eggs collected from the Fabacae / Menispermacae in the sealed room, or on Armyworm eggs. &lt;br /&gt;
* Rearing the parasitic wasps Fopius arisanus and/or Diachasmimorpha longicaudata to parasitize Tephritids (fruit flies). Tephritid eggs should be collected from fruits in the sealed room. To prevent the fruit fly eggs from drying out, the eggs should be kept on a moist substrate. To prevent molding, a constant air current should be provided during the time required for parasitoid development.&lt;br /&gt;
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The second phase may be more labour intensive. To rear Tetrastichus sp. and Euplectrus sp. or Trichogramma sp. for the purpose of parasitizing scales, moths, midges and longicorn beetles, one most collect eggs from all species in the field. Instead, one may also rear subject pests.&lt;br /&gt;
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As an alternative for green lacewings, one may use the combination of Cryptolaemus montrouzieri (mass reared on Plancoccus citri on potatoes or squash [http://www.arabscientist.org/english/page/11/], for predating on scales) and Orius insidiosus (mass reared on [http://www.waiwiki.org/index.php?title=Armyworm Armyworms] (which are best reared on Bermudagrass), for predating on aphids, thrips and small caterpillars). This allowes for a more targeted approach (if there is only 1 pest, eg scales). This may alsobe a better combination (together with Praying mantids) because adult Praying mantids prefer to predate on lacewings (15 mm long) over the much smaller C. montrouzieri (4 mm) and O. orius (3 mm).&lt;br /&gt;
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=Diseases=&lt;br /&gt;
Bugs cause damage to the mango tree, which makes the tree more susceptible to disease. Many bugs are also disease vectors; they transport the viruses, fungi etc. to the mango tree. Successfully combatting mango bugs, is therefore key to prevention and control of mango diseases. Using chemicals to fight mango diseases and/or bugs, however, may actually result in killing natural enemies of the bugs that spread disease.&lt;br /&gt;
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==Anthracnose==&lt;br /&gt;
[[Image:Anthracnose.jpg|thumb|left| Anthracnose [http://www.indiancropdiseases.com/mango.aspx]]]&lt;br /&gt;
[[Image:Anthracnose-2.jpg|thumb|right| Anthracnose [http://hawaiiplantdisease.net/cpg/displayimage.php?pid=397 © Nelson]]]&lt;br /&gt;
Anthracnose is caused by the fungi Colletotrichum acutatum [http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2003.93.5.579] and Glomerella cingulata [http://www.idosi.org/wjas/wjas4(6)/8.pdf][http://www.jofamericanscience.org/journals/am-sci/am0608/46_3350am0608_361_367.pdf]. It particularly affects young shoots, flowers and fruits. The disease is stimulated by high humidity, frequent rains or mists, and a temperature range of 24 to 32°C. The results of this disease are black spots on panicles, leaf spot, blossom blight, withered tip, twig blight and fruit rot. Particulary young and tender shoots and foliage are affected, causing die back of young branches. Wounded twigs may also be infected and may sometimes die off. Fruits develop black spots and rotting and young fruits may shrivel and prematurely drop. If the entire inflorescence is destroyed, no fruits will set. &lt;br /&gt;
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[u]Where?[/u] It widely occurs in the orchard and in storage, Anthracnose has been reported in Argentina, Florida, Hawaii, India, Pakistan, Philippines, Sri Lanka, South Africa and Trinidad.&lt;br /&gt;
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[u]Control[/u]: Burn all fallen leaves. Prune affected twigs and burn them. Apply Bordeaux paste on cut ends. To prevent blossom infection, spray twice at 15 days interval during flowering with Carbendazirn (Bavistin 0.1%). To prevent foliar infection, spray copper fungicides (0.3%) twice a week. Apply a teaspoon of dish detergent per spray load to make the copper sulfate stick. Prevent the copper sulfate from dripping on the ground to prevent contamination of the soil and the roots of the tree.&lt;br /&gt;
Picked mangoes should be submerged for 15 minutes in hot water (52°C) with Carbendazim (0.1%). One may also dip the mangoes in 500 ppm Benomyl and 900 ppm Thiobendazole. C. acutatum is also effectively inhibited by the synthetic strigolactone GR24.[http://www.ncbi.nlm.nih.gov/pubmed/21688170]&lt;br /&gt;
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==Bacterial Canker==&lt;br /&gt;
Bacterial black spot (bacterial canker) is a bacterial disease caused by Xanthomonas campestris pv. mangiferaeindicae. It affects many varieties. Initially, irregular small water soaked lesions will appear on the leaves. These will extend into patches of dead tissue. The leaves will turn yellow and die. The lesions will also appear on the the stalk of the leaves, twigs and fruits. The fruits will then turn brown and black, and burst open. The released fluid is filled with bacteria that will contaminate the rest of the tree or other fruits in storage.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Agrimycin-100 (0.01%) or Streptocycline (0.01%), 3 times at 10 days interval. Subsequently spray Copper Oxychloride (0.3%) or Carbendazim (Bavistin 0.1%) once a month.&lt;br /&gt;
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==Die Back==&lt;br /&gt;
Die back (Botryodiplodia (Lasiodiplodia) theobromae aka Physalospora disrupta/quercuum/rhodina/glandicola) may occur any time of the year. It is characterized by advancing discoloration and darkening of the bark. It extends along the veins of the leaves, causing browning and upwards rolling of the margins. Eventually the leaves shrivel and fall. Twigs and branches dry and defoliate, and may drain a yellow-brown gummy fluid. It may look as if the tree has been exposed to a bush fire.&lt;br /&gt;
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[u]Control[/u]: Prune diseased twigs 10 cm below the affected area. Paste the cut ends with Copper Oxychloride (0.3%) and also spray Copper Oxychloride (0.3%) on affected trees.&lt;br /&gt;
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==Diplodia Stem-end Rot==&lt;br /&gt;
This fungus (Lasiodiplodia theobromae) may enter the mango where the skin or stem has been injured mechanically. It will form a black circle around the pedicel.&lt;br /&gt;
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[u]Control[/u]: Prevent mechanical injuries. After picking, submerge mangoes for 15 minutes in hot water (53°C) with Carbendazirn (0.1%).&lt;br /&gt;
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==Mango Black Blight==&lt;br /&gt;
Black blight is caused by a fungus (Capnodium mangiferum).&lt;br /&gt;
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==Mango Blight==&lt;br /&gt;
Mango blight is caused by Erwinia bacteria (Enterobacteriaceae). Many spots appear on the leaves which cause a reduction in growth and yield. &lt;br /&gt;
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[u]Control[/u]: Use Dithane M 45 at the rate of 1.7g/L (450 L water per acre).&lt;br /&gt;
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==Mango Malformation==&lt;br /&gt;
Mango malformation (Fusarium mangiferae) has been reported in Australia, Bangladesh, Brazil, Central America, Cuba, Egypt, India, Israel, Malaysia, Mexico, Pakistan, South Africa, Sudan, Switzerland, UAE and the USA. It is a disease caused by fusarium species [http://www.mycologia.org/content/94/4/722.full][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.6.456] (F. subglutinans from Egypt, Florida, Israel, Malaysia and South Africa, F. sterilihyphosum from Brazil and South Africa, and Fusarium sp. nov. and F. proliferatum from Malaysia)[http://www.ncbi.nlm.nih.gov/pubmed/18943188][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-96-0667 Free Full Text]. The disease is stimulated by relatively cool conditions; 10 to 27°C, and merely survive hotter conditions. In this disease the leaves and inflorescence of the mango tree are badly deformed and gradually dry up. There is no fruit setting and hence no production. &lt;br /&gt;
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[u]Control[/u]: Various fungicides, including Captan, Benomyl and Thiram have been reported to be effective to some extend. &lt;br /&gt;
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==Mango Scabs==&lt;br /&gt;
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Mango Scabs (Denticularia mangiferae aka Sphaceloma mangiferae aka Elsinoë mangiferae) survives on young tissues (young leaves, shoots, flower, fruits) of the mango tree only. Adult tissue is not susceptible. Its spores (and their germination) requires rain splash and free water to produce new infections.[http://www.cerambycoidea.com/titles/affa2004.pdf] Moisture conditions stimulate its growth. Though this parasite is not a fungus but in a persistent parasitic relationship with the mango tree, it produces symptoms that look somewhat familiar to those of anthracnose (a fungus) infections. It starts with small light- or dark brown or grey spots on the underside of leaves and fruit. These spots get bigger and darker with time. In the center of this lesion a cracked texture will appear. If the host tissue survives, lesions may develop into scar tissue. When infestation is severe, there is loss of leaves and fruit.&lt;br /&gt;
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[u]Control[/u]; Remove and destroy all fallen leaves, twigs, branches etc. Spray copper hydroxide or copper oxychloride on a very regular basis, particularly in the rainy season. Without chemical control, losses as high as 90% have been observed.[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Phoma Blight==&lt;br /&gt;
Phoma blight (Phoma glomerata) is a fungus that affects the fibre in the top layer of old leaves. It produces small, irregular, angular lesions. As the lesions grown, the colour changes from yellow-brown to cinnamon. Many small spots may form overwhelming patches and result defoliation of affected leaves. Phoma glomerate very effectively produces an enzyme that converts the remainders (glucose) of fibre (from the leaves) into it the main structural component (N-acetyl-D-glucosamine)[http://www.ncbi.nlm.nih.gov/pubmed/15553782] in its cell walls, thus enabling its own growth.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Benomyl (0.2%), and 20 days later spray 0.3% Miltox (Copper Oxychloride plus Zineb). Quadris (azoxystrobin) combined with thymol at a non-fungitoxic concentration produces much higher growth inhibition of Phoma glomerata than the fungicide alone.[http://www.ncbi.nlm.nih.gov/pubmed/22408641]&lt;br /&gt;
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==Powdery Mildew==&lt;br /&gt;
Powdery mildew (Oidium mangiferae) affects virtually all mango varieties. Characteristically, a white superficial powdery fungal grows on the inflorescence and tender leaves, stalk of panicles, flowers and young fruits. The affected flowers and fruits will pre-maturely drop, or fruit setting does not occur at all. The disease is initiated and developed by warm (&amp;gt;17°C) dry weather, but during the flowering stimulated by prolonged rains or mists and by cool nights. The disease has been reported in Brazil, India, Jamaica, Pakistan, Sri Lanka, South Africa and the U.S.A. &lt;br /&gt;
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[u]Control[/u]: Alternately spray wettable sulphur (0.2% ; 2 g Sulfex/L), Tridemorph (O.1% ; 1 ml Calixin/L) and Bavistin (0.1%) at 15 days interval. Start spraying when the panicle (flower cluster) starts to emerge. Or spray with Carbendazim (0.1%) or Tridemefon (0.05%) or wettable sulphur (0.3%) alone (3 days in a row).&lt;br /&gt;
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[u]Control-2[/u]: Similar to A. quisqualis AQ10 Biofungicide in commercial use for biocontrol of powdery mildew, Phoma glomerata (see [http://www.waiwiki.org/index.php?title=Mango#Phoma_Blight Phoma Blight] above) may act as mycoparasite of powdery mildew. Phoma glomerata can colonize and suppress development of powdery mildew.[http://www.ncbi.nlm.nih.gov/pubmed/10618259] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91841/ Full free text]&lt;br /&gt;
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==Red Rust==&lt;br /&gt;
Red rust (Cephaleuros virescens aka C. parasiticus) initially causes round and slightly elevated green-grey spots mainly on leaves. They turn into rusty red spots and may form bigger patches. This reduces the photosynthetic capacity of the leaves. In severely infected trees, twigs will remain stunted, the bark and twigs will get thick and the leaves will dry up and drop off. &lt;br /&gt;
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[u]Control[/u]: Spray Copper Oxychloride (0.3%) 3 times.&lt;br /&gt;
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==Sooty Mould==&lt;br /&gt;
Sooty mould (Meliola mangiferae) may be the result of too many insects in your orchard. Scale insects, mealy bug and hoppers secrete honey dew. The honey dew sticks to the leaves, feeding fungal growth. This results in a black sooty mould over the entire surface of leaves and twigs, affecting the photosynthetic capacity of the leaves. Uncontrolled, the tree may completely turn black.&lt;br /&gt;
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[u]Control[/u]: Prune and burn the affected branches. Spray Wettasulf (0.2%) with+ Metacid (0.1 %) and gum acacia (0.3%). Ants feed on the honeydew excreted by soft scales, preventing accumulation of sooty moulds, but they also protect the scales from natural enemies.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
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	<entry>
		<id>http://www.waiwiki.org/index.php?title=Mango&amp;diff=4274</id>
		<title>Mango</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Mango&amp;diff=4274"/>
		<updated>2014-12-14T21:52:44Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
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&lt;div&gt;Comparable to oranges and clementines, mangoes are among the most all-round fruits, regarding nutrients. Mangoes are cultivated in (sub)tropical regions all over the world.&lt;br /&gt;
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=Low-fibre, sweet mango species=&lt;br /&gt;
If you use a juice extractor like the [http://www.thekitchn.com/product-review-hurom-slow-juic-131492 Hurom] / [http://versapers.fr/ Versapers] (masticating slow juicer), the stringy fibres from species like Tommy Atkins or Haden will clog your juicer (more so than pineapples). The chamber will fill up with long fibres that simply never get discarded, so that you need to empty and clean it after every few mangoes. With mangoes like Kent (#11 on the list), you may endlessly (&amp;gt;100 mangoes) continue juicing without having to empty or clean the chamber in between, because the short fibres are immediately discarded as pulp (as with apples, pears, clementines).&lt;br /&gt;
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==Anwar Ratol==&lt;br /&gt;
[[Image:Anwar_Ratol.jpg|thumb|right| Anwar Ratol [http://freshfruitpakistan.blogspot.nl/2013/04/anwar-ratol-top-variety-of-mango.html © H. Khalid]]]&lt;br /&gt;
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* #1 (aka Anwer Rathol, Anwar Retaul or Anwar Rataul)&lt;br /&gt;
* Small size (av. 180 g), medium thick skin&lt;br /&gt;
* Matures from green to pale bright yellow &lt;br /&gt;
* Very sweet (TSS 26-28%)&lt;br /&gt;
* Fibreless flesh, medium juicy&lt;br /&gt;
* Originally from Rataul, in Bagpat district, in the province of Uttar Pradesh, India. &lt;br /&gt;
* Harvested from June to July (India and Punjab in Pakistan)&lt;br /&gt;
* Keeps well in storage&lt;br /&gt;
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==Alphonso==&lt;br /&gt;
[[Image:Alphonso.jpg|thumb|right|]]&lt;br /&gt;
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* #2 (aka Alphanso, Bombay)&lt;br /&gt;
* Small, roundish / oblong (av. 200 g.)&lt;br /&gt;
* Smooth, (from green to) pale greenish-yellow to deep orange peel (deepening with ripening)&lt;br /&gt;
* Smooth, firm flesh. Intense reddish-orange colour&lt;br /&gt;
* Sweet, somewhat sour (17% sugar; sugar/acid ratio: 39; TSS 21-23%) and flavoured&lt;br /&gt;
* Virtually without fibre (1%), medium juicy&lt;br /&gt;
* Originally from Western India&lt;br /&gt;
* Main production in Maharashtra and Gujarat&lt;br /&gt;
* Highest quality in Sindhudurg, Ratnagiri (Natwarlal plantation), Raigad and Thane districts&lt;br /&gt;
* Harvested from early April to early July (India) and July (some districts in Sindh, Pakistan)&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
* Tolerates high humidity&lt;br /&gt;
* Good keeping quality (up to 4 weeks at 11° C)&lt;br /&gt;
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==Dashehari==&lt;br /&gt;
[[Image:Dashehari.jpg|thumb|right|]]&lt;br /&gt;
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* #3 (aka Dasheri, Doshehri, Aman Dusehri or Dussehri)&lt;br /&gt;
* Small, oblong (av. 175 g)&lt;br /&gt;
* Matures from light green to light yellow, thin skin&lt;br /&gt;
* Sweet (TSS 21-22%), lemon yellow flesh&lt;br /&gt;
* Fibreless, medium juicy&lt;br /&gt;
* Very small stone&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Dasheri, Uttar Pradesh, India.&lt;br /&gt;
* Harvested from early June to July, mainly Malihabad, Uttar Pradesh (and Punjab)&lt;br /&gt;
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==Bagan Pali==&lt;br /&gt;
[[Image:Bagan_Pali.jpg|thumb|right|]]&lt;br /&gt;
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* #4 (aka Begun Palli, Began Phali, Banganapalli or Baganpali)&lt;br /&gt;
* Large (av. 550 g.), oval&lt;br /&gt;
* Sweet (TSS 18-20%)&lt;br /&gt;
* Fibreless, little juicy, yellow flesh &lt;br /&gt;
* Matures from dark green to yellow light green or yellow-orange&lt;br /&gt;
* Thin, smooth, shiny skin&lt;br /&gt;
* Originally from Sindh, Pakistan and/or Banganapalle in Andhra Pradesh, India.&lt;br /&gt;
* Harvested from July to August (Pakistan)&lt;br /&gt;
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==Sindhri==&lt;br /&gt;
[[Image:Sindhri.jpg|thumb|right|]]&lt;br /&gt;
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* #5 (aka Sindhry)&lt;br /&gt;
* Medium to large (av. 300 g., av. 400 g in Pakistan)&lt;br /&gt;
* Elongated with pointy curve&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 36; TSS 15-17%), flavoured&lt;br /&gt;
* Little fibre (3% - 5%), little juicy&lt;br /&gt;
* From green to deep matt lemon yellow thin peel (somewhat wrinkly) when ripe&lt;br /&gt;
* Deep yellow-orange flesh; soft and melting&lt;br /&gt;
* Originally from Mir Pur Khaas in Sindh&lt;br /&gt;
* Harvested from late May to July (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Chaunsa==&lt;br /&gt;
[[Image:Chaunsa.jpg|thumb|right|]]&lt;br /&gt;
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* #6 (aka Sammar Bahisht Chausa)&lt;br /&gt;
* Large (av. 350 g.), oblong&lt;br /&gt;
* Sweet (18% sugar; sugar/acid ratio: 58; TSS 18-20%)&lt;br /&gt;
* Soft, succulent flesh &lt;br /&gt;
* Medium fibre, large stone, medium juicy (delicious for eating, too stringy for the slow juicer)&lt;br /&gt;
* Thin, pale, matt yellow-greenish peel (wrinkly and slightly deeper when ripe)&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Pakistan&lt;br /&gt;
* Harvested mainly in the Rahim Yar Khan and Multan (Sahiwal) districts of Punjab in Pakistan&lt;br /&gt;
* Harvested from June to early September (Pakistan)&lt;br /&gt;
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==Maya==&lt;br /&gt;
&lt;br /&gt;
* #7&lt;br /&gt;
* Medium, round (av. 300 g.)&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 42; TSS 16-17%) and flavoured&lt;br /&gt;
* Red blush peel (with yellow tinge when ripe)&lt;br /&gt;
* Smooth, deep/pale yellow flesh (soft when ripe)&lt;br /&gt;
* Medium fibre, very juicy&lt;br /&gt;
* Harvested from mid-June to early July (Gambia)&lt;br /&gt;
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==Gopalbogh==&lt;br /&gt;
&lt;br /&gt;
* #8 (aka Gopal Bhogue)&lt;br /&gt;
* Small size (average 190 g.)&lt;br /&gt;
* Yellow-green peel, with small seeds&lt;br /&gt;
* Virtually without fibre&lt;br /&gt;
* Very sweet (21% sugar)&lt;br /&gt;
* Good quality mainly grown in Rajshahi region, especially in Chapainawabganj district (Bangladesh).&lt;br /&gt;
* Harvest from mid May to mid June (Bangladesh)&lt;br /&gt;
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==Sweet Elena==&lt;br /&gt;
[[Image:Sweet_Elena.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #9&lt;br /&gt;
* Large sized, ovoid (av. 350 g.)&lt;br /&gt;
* Thin, orange peel&lt;br /&gt;
* Yellow to orange flesh&lt;br /&gt;
* Little fibre&lt;br /&gt;
* Sweet (19% sugar)&lt;br /&gt;
* Originally (as a carabao variety) from Sta. Cruz, Zambales (Philippines)&lt;br /&gt;
* Harvest from March to April (Locloc, Palauig in northern Zambales, Philippines)&lt;br /&gt;
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==Ataulfo==&lt;br /&gt;
[[Image:Ataulfo.jpg|thumb|right|]]&lt;br /&gt;
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* #10 (aka Adaulfo, Adolfo, Champagne, Manila or honey mango, related to Alphonso)&lt;br /&gt;
* Small, oblong shape (av. 225 g.)&lt;br /&gt;
* With ripening, peel turns from pale green to gold-yellow (yellow to orange and wrinkles when fully ripe)&lt;br /&gt;
* Deep yellow flesh (gold when ripe), little fibre and thin pit&lt;br /&gt;
* Smooth, sweet (15% sugar), and buttery / creamy flesh&lt;br /&gt;
* Spicy flavour &lt;br /&gt;
* Best temperature is 28°C, tolerates 1100 to 3000 mm annual rainfall &lt;br /&gt;
* Originally from Chiapas, Mexico&lt;br /&gt;
* Harvest from mid-October to late December (Ecuador); February to August (Ghana)&lt;br /&gt;
* May be stored in the fridge for 2 weeks&lt;br /&gt;
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==Kent==&lt;br /&gt;
[[Image:Kent.jpg|thumb|right|]]&lt;br /&gt;
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* #11&lt;br /&gt;
* Large, rounded oval (500 to 800 g.)&lt;br /&gt;
* Smooth, waxy thick peel (green with some yellow and red; yellow increases with ripening)&lt;br /&gt;
* Soft, melting, juicy flesh (from deep yellow to deep orange when fully ripe)&lt;br /&gt;
* Limited fibre (dry weight: 0.4 - 2%), small seed&lt;br /&gt;
* Sweet (13% sugar; 14ºBrix [http://www.m.elewa.org/JAPS/2013/17.3/5.pdf])&lt;br /&gt;
* Originally from Mexico / Florida&lt;br /&gt;
* Harvest from January to March and May to August (Ghana)&lt;br /&gt;
* Large tree (up to 20 m.)&lt;br /&gt;
* No storage keeping below 13°C&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
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=Mango Cultivation=&lt;br /&gt;
==Conditions==&lt;br /&gt;
The best soil for mango trees has a pH between 5.5 and 7.5 (high pH may cause leaf tip burn). The temperature range for growing mangoes is 15 to 40°C. Mango does best within a temperature range of 24 to 27°C. The Indian race is in general intolerant of humidity (susceptible to anthracnose), the Philippine race tolerates excess moisture. The life span of each tree is approximately 100 years. Economic bearing life is 30 to 50 years.&lt;br /&gt;
Mango trees need deep soil (up to 2 m) for their root systems. Grafted saplings yield a more superior quality of fruit. Fruits are borne by mango trees from 4 to 5 years after planting. Full bearing starts at 6 to 7 years.&lt;br /&gt;
Grafted mango plants start bearing blossoms one to two years after planting, provided that there is no high humidity or intense rain during the flowering period (dryness induces flowering).&lt;br /&gt;
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==Rootstock Propagation==&lt;br /&gt;
[[Image:Mango_grafting.jpg|thumb|left| [http://www.muftisays.com/blog/ummi+taalib/2718_20-03-2012/shaikhmurid-relationship.html © ummi taalib]]] &lt;br /&gt;
[[Image:Grafted_seedling.jpg|thumb|centre| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
[[Image:Grafted_seedlings.jpg|thumb|right| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
Trees grown from mango seeds (and naturally, sexually propagated) will be very different from the tree those mangoes came from (such &amp;#039;wild mangoes&amp;#039; usually taste bad). That is why rootstocks (raised from seeds) are asexually propagated. Matured seedlings (about 60 cm tall) are grafted by inarching; a bud from a tree producing good quality mangoes is artificially attached to the rootstock. This will create a new tree with two parts: the lower (wild) part and the upper part, which is an exact copy of the tree you took this bud from. Any bud growing from the lower part needs to be pruned off immediately. The upper part needs to grow and produce branches, and eventually fruits. During the first month after planting, the young trees need to be irrigated every 3 days. During the next 2 months, they need to be irrigated once a week. After that they should not be watered for a couple of months. Each year they need a dry season of a few months. Young plants need 10 to 30 kg organic manure per plant. Don&amp;#039;t use a chemical fertilizer on young plants. Once the tree is 2 years old, you may start using a controlled release fertilizer (with higher nitrogen), like 12-5-9 or 12-8-34 (= ratios N:P:K); 1 tbsp. in 1 L warm water / meter tree height. Or just manure.  &lt;br /&gt;
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Rootstock branches should always be pruned off. To prevent viral infections, only prune using a very sharp, sterilized knife (sterilize on the spot with a propane torch).&lt;br /&gt;
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==Production technology== &lt;br /&gt;
Space mango trees about 12 meters apart. Adult mango trees need about 500-750 mm of water per year. In the wet season irrigated once per 8 to 10 days. In the dry season once per 15 to 20 days. Mango trees need dry and wet spells alternately (also kills various pathogens). Don&amp;#039;t fertilize mature trees during fruit bearing, and not too much irrigation. If during the blooming season the soil contains much nutrients (fertilizer) and water, the tree will grow, but not flower and bear fruits. &lt;br /&gt;
You may either:&lt;br /&gt;
* A: Apply 80 to 100 kg manure per year (eg humanure from [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets]), after all fruits are picked. Or:&lt;br /&gt;
* B: Apply 3-4 kg SSP, 2-3 kg Potassium Sulphate and 2-3 kg Urea before flowering. Plus 2-3 kg Urea in 2 doses after fruit setting. Or:&lt;br /&gt;
* C: Use a controlled release fertilizer once a year, such as 4-4-8 (= ratios N:P:K), after all fruits are picked (1 tbsp. in 1 L warm water / meter tree height).&lt;br /&gt;
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After fruit harvest, prune off diseased, dried, broken branches and those touching the ground. Every 3 to 4 years about 15 to 20% of old wood should be removed.&lt;br /&gt;
Picking should be done when the fruit is fully developed and mature. Natural drop of the fruit is the main indication that the fruit is ready for picking. Expected yields vary from 40 to 100 kg per tree.&lt;br /&gt;
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One cannot rear any animals (except for carnivores) on land with mango trees, as mango leaves are deadly poisonous.&lt;br /&gt;
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=Bugs=&lt;br /&gt;
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==Aphids==&lt;br /&gt;
[[Image:Propylea_quatuordecimpunctata.jpg|thumb|right| Predatory ladybird [http://www.colpolon.biol.uni.wroc.pl/propylea%20quatuordecimpunctata.htm]]]&lt;br /&gt;
[[Image:Aphids_on_mango.png|thumb|left| Aphids [http://www.infonet-biovision.org/default/ct/124/crops#_1804_1202 © Varela]]]&lt;br /&gt;
[[Image:Mallada_sp_2.jpg|thumb|right| Mallada sp. © Texas University]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
&lt;br /&gt;
Aphids (plant lice; Toxoptera odinae) are small (1-4 mm), living in clusters. They pierce plant tissue and suck the sap of the leaves, which may cause the leaves to bend, twist or roll up. Aphids also transmit plant viruses. Some species produce winged offspring, &amp;quot;alates&amp;quot;, that may readily disperse. Dry (and warm) weather stimulates increases in aphid numbers. Some species of ants (eg Dolichoderus cuspidatus and Formica aerate) protect and &amp;#039;farm&amp;#039; aphids, feeding on the honeydew released by the aphids&amp;#039; alimentary (gut) canals. Insects secreting honeydew may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould] &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Plant flowering plants at the boarders to attract beneficial insects. Plant &amp;#039;trap crops&amp;#039; (dill, nasturtiums, timothy grass) to monitor aphid numbers. Check the trap crops every 3 days. Infested trap crops need to be burned. Aphid&amp;#039;s natural enemies include predatory ladybirds (eg Propylea quatuordecimpunctata), hooverfly larvae, parasitic wasps, aphid midge larvae, crab spiders (Thomisidae) and lacewings (Neuroptera). Exposing aphid populations to natural predators may be successful particularly when its hot, because once bitten they release an alarm pheromone and the others all jump off the plant. Jumping aphids may experience a heat shock (&amp;gt; 35°C) since the ground may be much warmer than the shady tree. This heat shock usually sterilizes the aphids (killing the bacteria on aphids that provide nutrients essential for aphid reproduction). [http://www.sciencedaily.com/releases/2007/04/070419172046.htm]&lt;br /&gt;
&lt;br /&gt;
[u]Crab spiders (Thomisidae)[/u]; Crab spiders are ambush hunters (they don&amp;#039;t build webs) that not just eat aphids, but may also hunt ants that farm aphids. Some of these crab spiders, Xysticus sp, may however not just eat ants, but also predatory beetles (that eat aphids).&lt;br /&gt;
&lt;br /&gt;
[u]Green lacewings (Chrysopidae)[/u]; One may buy millions of lacewings (as captive-bred eggs) for biological pest control, as reared for that purpose in many countries. A female lacewing may plant over 100 eggs on plants infested with aphids. These eggs hang on a thin 1 cm long stalk, most often under a leaf. The larvae of most species of lacewings are specialised predators that eat aphids (and coccids such as mango scales, and caterpillars). These larvae sway their heads from left to right, and back, until they strike something they can eat. They have mouthparts that can pierce and suck the aphids. Many (adult) lacewing species also feed on nectar and pollen, but particularly Chrysopidae (eg Mallada signata, Mallada boninensis) are mainly predatory (one adult may eat 15 aphids per day). One may attract Chrysopidae by planting crops that attract them (mainly Asteraceae and Apiaceae), such as calliopsis (Coreopsis), cosmos (Cosmos), sunflowers (Helianthus), dandelion (Taraxacum), dill (Anethum) and angelica (Angelica). &lt;br /&gt;
&lt;br /&gt;
[u]Chemicals[/u]; If you have no other option and immediate action is required: Use Folido 50% EC at the rate of 0.45 L per 450 L water / acre. (also kills natural aphid enemies)&lt;br /&gt;
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==Fruit Flies==&lt;br /&gt;
[[Image:Fruit_fly_maggots.jpg|thumb|centre| Maggots [http://www.infonet-biovision.org/default/ct/124/crops © A. M. Varela]]]&lt;br /&gt;
[[Image:Bactrocera_invadens.jpg|thumb|left| B. invadens [https://www.flickr.com/photos/iaea_imagebank/6776806998/ © IAEA]]]&lt;br /&gt;
[[Image:Fopius_arisanus.jpg|thumb|right| F. arisanus [http://www.tephritid.com/digital.php?act=page&amp;amp;pid=28&amp;amp;id=25 © tephritid.com]]]&lt;br /&gt;
[[Image:Ceratitis_capitata.jpg|thumb|left| C. capitata [http://josedotinzulza.edublogs.org/2013/04/21/how-much-damage-can-make-a-fly/ © José Inzulza]]]&lt;br /&gt;
[[Image:Diachasmimorpha_longicaudata.jpg|thumb|right| D. longicaudata [http://www.oocities.org/brisbane_parawasps/FruitFlyParasitoid.htm]]]&lt;br /&gt;
[[Image:Ceratitis_fasciventris.jpg|thumb|left| C. fasciventris [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Oecophylla_longinoda.jpg|thumb|right| O. longinoda [http://www.alexanderwild.com/Ants/Taxonomic-List-of-Ant-Genera/Oecophylla/i-tMfJXKG © A.Wild]]]&lt;br /&gt;
[[Image:Ceratitis_cosyra.jpg|thumb|left| C. cosyra [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Jackson_trap.jpg|thumb|right| Jackson trap]]&lt;br /&gt;
[[Image:Ceratitis_rosa.jpg|thumb|left| C. rosa [http://wzciq.wenzhou.gov.cn/art/2011/1/12/art_6811_63595.html © wzciq]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis eating fly [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
In 2013, the export of mangoes from Ghana and 27 other African countries was banned from major international markets mainly due to fruit fly infestation. The European Union imposed a ban from May 1 2014, on import of mangoes from India, also due to fruit fly infestation. &lt;br /&gt;
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The fruit flies (aka peacock flies; 4-7 mm long) that may form a very severe mango cultivation pest are not the Drosophilae (2-4 mm long), but the colourful Tephritidae family (&amp;gt; 5000 species). These attack mango fruits throughout the season and lay their eggs right under the (affected) skin of mature green or ripe fruits. They have three generations and multiply very rapidly. Within one or two days the eggs hatch into white maggots. The maggots feed on the fruit, which starts to rot. After 4 to 17 days, the maggots make holes in the skin and leave the fruit to pupate. &lt;br /&gt;
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The main fruit fly pests of mango in Africa are Ceratitis capitata (Medfly), C. fasciventris, C. rosa and C. cosyra (the most destructive). C. cosyra is however rapidly displaced by Bactrocera invadens (endemic to Sri Lanka)[http://www.ncbi.nlm.nih.gov/pubmed/19610411][http://www.ncbi.nlm.nih.gov/pubmed/22251685], being predominantly a low-land pest.[http://www.ncbi.nlm.nih.gov/pubmed/16923206] B. invadens populations quickly increase with the onset of the raining season[http://www.ncbi.nlm.nih.gov/pubmed/19449630] and also lay eggs in young fruits. Fruit fly populations peak in the late dry season.[http://www.ncbi.nlm.nih.gov/pubmed/17598527]&lt;br /&gt;
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[u]Control[/u]: Collect all the fallen and affected fruits and bury them deep (&amp;gt;50 cm) into the soil. Or you can use them to breed maggots in a sealed room for fish feed. Natural enemies of tephritids include Diapriidae (tiny wasps; &amp;lt;8mm), Braconidae (also parasitoid wasps) and Oecophylla longinoda (Latreille; a weaver ant). &lt;br /&gt;
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C. cosyra, but particularly C. fasciventris and Medfly may be effectively controlled by using the host-marking pheromone.[http://www.ncbi.nlm.nih.gov/pubmed/23356072] B. invadens [http://www.ncbi.nlm.nih.gov/pubmed/24293246], the Mediterranean fruit fly (C. capitata) and the Mexican fruit fly (Anastrepha ludens) may be effectively controlled by the &amp;#039;sterile insect technique&amp;#039; (releasing overwhelming numbers of sterile insects). The effects of sterile flies are greater than those for parasitoid wasps, but they are complementary.[http://www.ncbi.nlm.nih.gov/pubmed/15568340] &lt;br /&gt;
Medfly eggs and instars (right under the peel) may be killed by immersing Ataulfo mangoes for 95 min in warm water at 47°C, also positively modifying pH (producing more palatable fruits), but can also produce a loss of firmness and weight (5%).[http://www.ncbi.nlm.nih.gov/pubmed/23356057] B. invadens is no more heat tolerant than Medfly.[http://www.ncbi.nlm.nih.gov/pubmed/21404834] &lt;br /&gt;
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[u]Bait traps[/u]; &lt;br /&gt;
Pheromone traps (85% methyl-eugenol, 5% Naled (insecticide), 10% saturated sugar) can be used for trapping male flies (Jackson trap). A three-component attractant (ammonium acetate, putrescine, and trimethylamine lures) may be used to trap female population of Medfly. (No wind: 28 m. range).[http://www.ncbi.nlm.nih.gov/pubmed/21061993] Malathion-bait sprays and Phloxine B (a xanthene dye; D&amp;amp;C Red #28) are equally effective.[http://www.ncbi.nlm.nih.gov/pubmed/11777044]&lt;br /&gt;
Of six commercial food-based attractants, Nulure captured the greatest proportion of females: 74% compared with 51-68%. But Mazoferm E802 (with or without Spinosad) and Torula yeast captured 2.4-2.6 times more females and 3.4-4.0 times more males than Nulure (also more effective than GF-120, Hymlure and Biolure).[http://www.ncbi.nlm.nih.gov/pubmed/24665714]&lt;br /&gt;
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[u]Fopius arisanus (Sonan)[/u]; F. arisanus is a tiny but most effective[http://www.ncbi.nlm.nih.gov/pubmed/12241567] and the most dominant parasitoid wasp. As parasitism increases, fruit fly infestation decreases.[http://www.ncbi.nlm.nih.gov/pubmed/17598524] Of 3 commonly used bait sprays, which all suppress fruit fly populations, Spinosad and Phloxine B bait sprays are less harmful to these wasps than Malathion bait spray. [http://www.ncbi.nlm.nih.gov/pubmed/11561838]   &lt;br /&gt;
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[u]Diachasmimorpha longicaudata[/u]; D. longicaudata (longtailed fruit fly wasp) is the most important parasitoid wasp used as part of integrated pest management programs against fruit flies such as B. invadens and Ceratitis species. They lay one or more (when hosts are scarce) eggs in fruit fly larva.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014816/] Introduction of D. longicaudata poses minimal competitive risk to F. arisanus.[http://www.ncbi.nlm.nih.gov/pubmed/12241567]&lt;br /&gt;
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[u]Oecophylla longinoda[/u]; O. longinoda is a predator weaver ant that may eat whatever insect or bug they may catch, including caterpillars, beetles, honey bees, driver ants (Dorylus nigricans Ill.) and larvae. O. longinoda is a natural enemy of fruit flies as it eats their larvae. This weaver ant is fiercely territorial and will keep other species of ants (like aphids-farming black ants) at bay. The ants continuously patrol the tree that they live in and may also catch egg-laying female fruit flies in the act, and eat them. B. invadens and C. spp numbers on Kent mangoes may be greatly reduced by Oecophylla longinoda.[http://www.ncbi.nlm.nih.gov/pubmed/17598527] &lt;br /&gt;
Weaver ants, however, also feed on honeydew. They may farm a wide range of honeydew-producing Homoptera (bugs with sucking mouthparts, including scales, aphids and mealybugs), but are mostly associated with Coccids (Saissetia; no virus vector). Only when other Coccids are scarce or unavailable, O. longinoda may farm aphids and mealybugs (unarmoured scale insects that are virus vectors). Mainly in small trees and shrubs, the mechanical damage caused by Coccids is related to the size of the attendant ant colony. Dense populations of O. longinoda are supported by interplanting with clove.[http://www.cabdirect.org/abstracts/19540500227.html;jsessionid=36A454B839F0DEEADFA83F99A7206E6D]&lt;br /&gt;
For the protection of their harvested honeydew, these weaver ants weave a translucent silken tent around living leaves. They use the silk produced by their larvae. It is claimed that pheromones produced by the weaver ants repel egglaying fruit flies. A study in Senegal, however, found that pheromones produced by O. longinoda did hardly repel egglaying females of B. invadens.[http://www.ddrn.dk/filer/forum/File/Abstract_Christina_Abel.pdf]&lt;br /&gt;
On the other hand, trees colonized by O. longinoda (in Ghana) had only 6 to 10% fly infestation, compared to 3% fly infestation in trees treated with Cypermethrin plus Dimethoate. (1614 mg per tree)[http://ugspace.ug.edu.gh/handle/123456789/2387] &lt;br /&gt;
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[u]Praying mantids[/u] may also catch and eat fruit flies. Praying mantids are opportunist predators and do not farm any insects or other bugs. Particularly young mantids may not yet be too large to prey on fruit flies (tephredids). The younger and/or smaller the praying mantids are, the smaller the insects (and any other living creature they may catch) they prey on. Praying mantid nymphs initially feed on aphids and fruit flies (drosophila), and as they grow, they skip to larger preys, such as tephritids, and eventually moths and even much bigger preys.&lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option, GF-120 (Dow chemical) may greatly (81-89% after 7-10 weeks) reduce the number of B. invadens pupae per kg mango.[http://www.ncbi.nlm.nih.gov/pubmed/19449630] Or use Dioptries 80% at the rate of 1 L in 450 L water or Malathion 57% at the rate of 0.5 L to 450 L water / acre. Broad-spectrum insecticides are most damaging to fruit flies&amp;#039; natural enemies such as parasitoid wasps.[http://www.ncbi.nlm.nih.gov/pubmed/19886446] &lt;br /&gt;
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==Long-horn Beetles==&lt;br /&gt;
[[Image:Stem_borer.jpg|thumb|left| Stem Borer [http://www.bitterrootrestoration.com/mango/stem-borer-batocera-rufomaculata.html ©]]]&lt;br /&gt;
[[Image:Dastarcus_helophoroides.jpg|thumb|right| D. helophoroides[https://www.ffpri.affrc.go.jp/labs/seibut/bcg/bcg00284.html ©]]]&lt;br /&gt;
[[Image:Batocera_rufomaculata_2.jpg|thumb|left| Batocera rufomaculata [http://agritech.tnau.ac.in/crop_protection/crop_prot_crop_insectpest%20_Mango_pest&amp;amp;disease.html © TNAU]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Batocera_rubus.jpg|thumb|left| Batocera rubus [http://163.20.112.34/~afu/77v.htm © ]]]&lt;br /&gt;
[[Image:Batocera_baby.jpg|thumb|left| baby Batocera [https://www.flickr.com/photos/bondingtool/14291758294 © Samantha - thebondingtool.com]]]&lt;br /&gt;
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* [u]The (red-spotted) Stem Borer (Batocera rufomaculata)[/u], is a large long-horned beetle (up to 5 cm long body). It cuts incisions on the bark and lays its eggs (up to 200) inside the cuts or cracks of the (damaged) tree bark, trunk or cavities (eg exposed roots). The beetles feed on the bark of living trees and eat the green of young shoots. The grub of the stem borer pupates and feeds inside the stem of mango trees (and other fruit trees, including durian, figs, avocado, jackfruit and cashew nuts), creating a tunnel towards the surface (up to 2 cm in diameter), eventually becoming a beetle. Sap (from the tree) and frass (or coarse sawdust, from the larvae) may drip from the holes. Older larvae may also tunnel deeper in the trunk or branches. This causes drying of branches, and the tree may die. The incidence of infestation of trunk borer is highly influenced by maximum temperature. Infestation is highest when temperature and humidity are high.[http://www.ncbi.nlm.nih.gov/pubmed/24498801] For reproduction, it needs to lay its eggs in a stem that is at least 7 cm in diameter. The Stem Borer has been observed in the west coast of Africa, Madagascar, Mauritius, Réunion, Seychelles, Turkey, Israel, Syria, Iraq, Iran, Pakistan, India, Bangladesh, Sri Lanka, China, Nepal, Thailand, Indonesia, Malaysia and the West Indies. Adults may live up to 4 months. Full grown larva may be 8 to 10 cm long, and may live up to a year, causing a lot of damage. Larval and prepupal + pupal stage lasts about 280 and 24-29 days.[http://www.actahort.org/books/787/787_41.htm]&lt;br /&gt;
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* [u]The (yellow-spotted) Mango Longhorned Beetle (Batocera rubus)[/u] is also a wood borer and about equally large as the Stem Borer. Full grown larvae are 6 to 8 cm long. It attacks various fruit trees, including mango, breadfruit and figs, but also bonsay trees, rubber trees and freshly felled timber. Batocera rubus has been observed in Pakistan, India, Bangladesh, China, Cambodia, Indonesia, Malaysia, Phillipines, Thailand, Taiwan and Vietnam.&lt;br /&gt;
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[u]Natural enemies[/u]; Tiny parasitoid wasps such as Oobius agrili sp.n. (1 mm long), Spathius agrili, Avetianella batocera, Tetrastichus planipennisi and Avetianella xystrocerae sp.n (1.5 mm long) have been shown effective against (the larvae of) some specific wood-boring beetles (Agrilus planipennis, Agrilus sexsignatus and Xystrocera globosa)[http://www.emeraldashborer.info/files/Zhang%20et%20al%202005%20Oobius_Leah.pdf][http://www.fs.fed.us/nrs/pubs/other/2013/2013_McManus_FHTET-13-01.pdf]. Batocera rufus (the mango longhorn beetle), is parasitized (its eggs) by  Louricia ovivora, gen. et sp. n.(a moth), and Ooencyrtus batocerae, sp. n.(a wasp) [http://cabdirect.org/abstracts/19360501471.html;jsessionid=A9E2E64A372836E8E24F2B4E97287DC9] Oobius batocera is an opportunistic parasytic wasp that parasitizes various insect eggs, including beetle eggs. [http://www.nhm.ac.uk/research-curation/research/projects/chalcidoids/database/synonyms.dsml?FamilyCode=EE&amp;amp;ValFamTrib=&amp;amp;VALGENUS=Oobius&amp;amp;VALSPECIES=batocerae&amp;amp;VALAUTHOR=(Ferri%E8re)&amp;amp;VALDATE=1936&amp;amp;ValidAuthBracket=false&amp;amp;HOMCODE=0&amp;amp;&amp;amp;listPageURL=listChalcids.dsml%3FSuperfamily%3DChalcidoidea%26Family%3DEncyrtidae%26Genus%3DOobius] &lt;br /&gt;
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There are also various mites that parasitize Batocera longhorned beetles.[http://www.jstor.org/discover/10.2307/3225250?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21103843600301] Tetrapolipus diastocerae n. sp., Tetrapolipus afrobatocerae n. sp., Tetrapolipus ramarajui n. sp. and Tetrapolipus seemani n. sp. are described. Tetrapolipus afrobatocerae has been observed in Africa, Tetrapolipus hunteri in Australia.[http://www.researchgate.net/publication/233099056_The_Genus_Tetrapolipus_(Acari_Podapolipidae)_Parasites_of_Tropical_and_Semitropical_Cerambycidae_(Coleoptera)_New_Distribution_Records_and_Descriptions_of_Four_New_Species] Tetrapolipus sulawesiensis is a mite that parasitizes Batocera herculus.[http://d.wanfangdata.com.cn/periodical_dwfl200202010.aspx] &lt;br /&gt;
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[u]Dastarcus helophoroides[/u]; This predator/parasitoid beetle (originally from China and Japan) is an important natural enemy of longhorned beetles. In the larval stage they attack various long horn beetles, including Batocera horsfieldi and Anoplophora glabripennis. Populations are attracted specifically by the frass of their original host. Eggs are laid twice a year, near the host entrance hole, frass-extrusion holes or larval tunnel walls, guided by semiochemicals. The freshly hatched (at about 21°C) first instars (0.7 mm in length) have legs, and actively move to the host. They bite and paralyze the host, and then suck hemolymph and degenerated tissues from the dead or paralyzed prey. Adults can live over 4 years. [http://link.springer.com/article/10.1007%2Fs10526-009-9224-y#page-1] Mass production is feasible if hatched larvae are fed cerambycid larvae until they are about 8 mm in length, and subsequently reared on artificial diet (silkworm pupa powder, dry yeasts, yeast extract, sucrose, peptone, squid liver oil, preservatives and distilled water). The full grown larvae are about 18 mm in length.[http://link.springer.com/article/10.1023%2FA%3A1009936609401#page-1] Adults don&amp;#039;t readily disperse; they need to be released on each tree separately. And even if you release them at 1.2 m height, their effectiveness decreases with increasing height in the tree. Yet their effectiveness may be very high; up to 85% host mortality. [https://www.ffpri.affrc.go.jp/labs/kanko/401-1.pdf]&lt;br /&gt;
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[u]Control[/u]; The stem borer is active during the night and attracted by light, so that light traps may be effective. Bacillus thuringiensis is a bacterium commonly used as a biological pesticide, and naturally present in the gut of various caterpillars. This bacterium is toxic to many insects. A specific strain of this bacterium, Bacillus thuringiensis ZQ-89 is also toxic to adult long-horned beetles.[http://www.ncbi.nlm.nih.gov/pubmed/21332894] Prevent the stem borer from laying eggs in the bark by applying a thick layer of coaltar on the stem. Or one may paint (at 0.5%) adult mango trees with monocrotophos or phosalone.[http://www.cabdirect.org/abstracts/19790379463.html] One may also specifically apply the insecticides to the potentially affected sites (cracks and cavities), and to twigs and young shoots to deter feeding by adults. One may poke with a hard wire to physically damage, and/or inject insecticides where larvae are suspected. Chloroform, ethyl acetate, Metasystox [demeton-S-methyl] and a mixture of petroleum and kerosene oil (at 5 ml/bore) and ethylene dibromide (at 3 ml/bore) gave 100% mortality of B. rufomaculata larvae.[http://www.cabdirect.org/abstracts/19881107545.html] Plaster the holes with wet clay, aluminium phosphide tablets (3 g/hole) and dichlorvos (0.1% spray).[http://www.cabdirect.org/abstracts/19790379463.html] Among the chemical treatments, Imidacloprid 17.8% SL, Thiamethoxame 25% WG was found best in management of mango stem borer.[http://www.gifre.org/admin/papers/gjbahs/management-vol-2-4-gjbahs.pdf]&lt;br /&gt;
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==Mango Hoppers==&lt;br /&gt;
[[Image:Idioscopus_niveosparsus.jpg|thumb|left| I. niveosparsus [http://163.20.112.34/~afu/641x.htm ©]]]&lt;br /&gt;
[[Image:Mallada_basalis.jpg|thumb|right| Mallada sp. [http://3insect.blogspot.nl/2012/06/mallada-basalis.html © Chen KunTsan]]]&lt;br /&gt;
[[Image:Idioscopus_clypealis.jpg|thumb|left| I. clypealis [http://163.20.112.34/~afu/641y.htm ©]]]&lt;br /&gt;
[[Image:Mallada_signata_3.jpg|thumb|right| Mallada signata [http://www.oocities.org/brisbane_lacewings/Chrysopidae.htm © Keith Power]]]&lt;br /&gt;
[[Image:Idioscopus_nitidilus.jpg|thumb|left| I. nitidilus [http://tech.groups.yahoo.com/group/pestnet/message/6414 ©]]]&lt;br /&gt;
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Mango hoppers:&lt;br /&gt;
* Idioscoynio chypeabis&lt;br /&gt;
* Idioscopus niveosparsus&lt;br /&gt;
* Idioscopus clypealis&lt;br /&gt;
* Idioscopus nitidulus&lt;br /&gt;
* Amritodus atkinsoni aka mango leafhopper&lt;br /&gt;
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Mango hopper nymphs as well as adults puncture and suck the sap of tender parts of the mango plant. Heavy drainage will cause the leaves to curl and dry. Infested flowers will shrivel and turn brown. Hoppers also secrete honeydew, which may cause sooty mould, inhibiting the leaves from obtaining energy from daylight. Sufficient spacing of trees and pruning of overlapping branches are essential in preventing hopper infestation, as shade (and high humidity) favour hopper multiplication.&lt;br /&gt;
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[u]Natural enemies[/u]:&lt;br /&gt;
Parasites: Aprostocetus sp., Gonatocerus sp. and Polynema sp. parasitize eggs of Idioscopus clypealis and Idioscopus nitidulus.[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
A preparation of the fungus Beauveria bassiana is also somewhat effective against mango hoppers. Chrysopa lacciperda [Plesiochrysa lacciperda] and Mallada boninensis predate on on nymphs of I. clypealis, I. nitidulus and Amritodus atkinsoni.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9]&lt;br /&gt;
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[u]Mallada boninensis[/u]; This green lacewing is an effective [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=5&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFQQFjAE&amp;amp;url=http%3A%2F%2Fwww.researchjournal.co.in%2Fonline%2FIJAS%2FIJAS%25203(2)%2F3_A-164-166.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNGZ9IidKvjO6s1zNs0HvbUMpKKPBg] generalist predator, feeding on mealy bugs (Mani and Krishnamoorthi, 1987), white flies (Selvakumaran et aI., 1996), bollworms and aphids (Kabissa et al., 1996) and on nymphs of Aphis gossypii, Aphis craccivora and Rhopalosiphum maidi [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=3&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEAQFjAC&amp;amp;url=http%3A%2F%2Fwww.ets-staffing.com%2Fcurrentbiotica%2Fjournals6-issueiii%2Fcb-6-3-short-notes-3.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNETNWfEOrZznoJjj0ZrFFOINJ2liw], blackflies, psylla and leaf miner. Green lacewings are recommended for the integrated pest management programme (Nehare et al., 2004). M. boninensis feeds well on Corcyra cephalonica eggs[http://cabdirect.org/abstracts/20113350526.html;jsessionid=69EA3BE15508EF9C029CE688C342FD4D] (laboratory host) C. cephalonica can be mass reared on Jowar along with groundnut, streptomycin, vitamin complex, Na and K salts.&lt;br /&gt;
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[u]Control[/u]: &lt;br /&gt;
Spray Imidacloprid (0.005%; 0.3 ml/L water), acephate 75SP (1.5 g/L water), Etofenprox (0.03%), Phosalone (1.5 ml/L water), Carbaryl (0.15%; 3 g/L water), Monocrotophos (0.04%), Phosphamidon (0.05%) or Methyl Parathion (0.05%). &lt;br /&gt;
* Spray 3 times; First spray should be given during the early stage of flower formation. The second spray should be given at a flower size of 6 to 8 cm, still before blooming. The third spray should be given after fruit setting, when the fruits are the size of a pea. Or:&lt;br /&gt;
* First spray should be given during the early stage of flower formation. The second spray should be given 2 weeks later. Or:&lt;br /&gt;
* Spray 3 times; First spray of imidacloprid (0.005%; 0.3 ml/L water) should be given during the early stage of flower formation. The second spray, thiamethoxam (0.005%; 0.2 g./L water) or acephate 75SP (1.5 g/L water) should be given at after fruit setting. If hopper infestation persists, the third spray, of carbaryl (0.15%; 3 g/L water) should be given prior to fruit maturation.&lt;br /&gt;
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Imidacloprid residues persist in peel for 60 days and in pulp for 50 days. Mature Dashehari fruits at harvest (after 85 days of spraying) were free from imidacloprid residues.[http://www.ncbi.nlm.nih.gov/pubmed/23196371]&lt;br /&gt;
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==Mango Midges==&lt;br /&gt;
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[u]Inflorescence Midge[/u]&lt;br /&gt;
[[Image:Platygaster_sp.jpg|thumb|right| Platygaster sp. [http://liuzhen.000space.com/insect/organism.php?id=1367&amp;amp;type=list]]]&lt;br /&gt;
[[Image:Inflorescence_midge.jpg|thumb|left| [http://www.bitterrootrestoration.com/mango/inflorescence-midge-erosomyia-indica.html Erosomyia indica ©]]]&lt;br /&gt;
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This midge (Erosomyia indica) lays eggs in new inflorescence (preventing flower and fruit set) and new leaves around it, and then the larvae will eat their way through. The maggots penetrate and feed on the tender parts of the plant, such as shoots, buds and flower buds. The flowers will dry and fall off. For pupation, the mature larvae will drop into the soil. The adult midge lives only one day and doesn&amp;#039;t cause any damage. Maggots from eggs laid on new fruits will eat their way into the fruit, which will turn yellow and drop.[http://www.actahort.org/books/231/231_15.htm] The inflorescence midge is an important pest in India in particular.&lt;br /&gt;
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Infloresence midge is parasitized by Platygaster sp., Eupelmus sp. and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19881115612.html;jsessionid=E4CDBFFBEE75C82EDA1BA2CA7B5F1DD2], which all also parasitize Gall Midges. The inflorescence midge is also parasitized by Aprostocetus sp.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9] and Mirufens longifunculata. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false]&lt;br /&gt;
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Another mango midge, Procystiphora mangiferae is parasitized by Pirene sp. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], which also parasitizes mango gall midges.&lt;br /&gt;
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Chemicals: If you have no other option, Parathion, Dimethoate, Phosphamidon and Endosulfan much reduced infestations on mango shoots.[http://www.cabdirect.org/abstracts/19740517480.html;jsessionid=59B14F930CB8EDFD2F1B50F1B04C7692] The most effective insecticide for control appeared to be phosphamidon (Dimecron), whether mixed with diazinon or not, as a foliar spray. [http://www.cabi.org/isc/abstract/19770549886]&lt;br /&gt;
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[u]Gall midges[/u]&lt;br /&gt;
[[Image:Mango_galls.jpg|thumb|left| [http://www.infonet-biovision.org/default/ct/124/crops © A.M. Varela]]]&lt;br /&gt;
[[Image:Eupelmus_urozonus.jpg|thumb|right| Eupelmus urozonus [http://www.biolib.cz/en/image/id150459/ © Miroslav Fiala]]]&lt;br /&gt;
[[Image:Mango_galls_P_matteiana.jpg|thumb|left| P. matteiana galls [http://www.pests.blogfa.com/8907.aspx © RJ Gagné]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Gall_midget_exit_holes.jpg|thumb|left| Exit holes [http://biostor.org/reference/55262 © RJ Gagné]]]&lt;br /&gt;
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There are various mango gall flies:&lt;br /&gt;
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- Erosomyia mangifereae or Procontarinia frugivora has been observed in the Philippines, India, West Indies and Brazil.&lt;br /&gt;
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- Asynapta sp. in West Indies.&lt;br /&gt;
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- Dasyneura mangifereae in Hawaii.&lt;br /&gt;
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- P. allahabadensis, P. biharana, P. brunneigallicola, P. viridigallicola, P. echinogalliperda, P. keshopurensis, P. mangifoliae, P. tenuispatha, P. amraeomyia and Dasyneura amaramanjarae have been observed in India (and some in Pakistan).&lt;br /&gt;
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- P. mangicola in China and Japan.&lt;br /&gt;
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- P. matteiana (1.5 mm long) in India, Kenya, Mauritius, Java and Réunion[http://biostor.org/reference/55262][http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=4&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFEQFjAD&amp;amp;url=http%3A%2F%2Fwww.geocities.ws%2Fmdce2005%2FMDCE2005%2F27-98_102.pdf&amp;amp;ei=HS5zU-P3DomXyQOd_IHgCA&amp;amp;usg=AFQjCNHlZtUS8qYFE2lCVSqh3ow0K4ukLg&amp;amp;sig2=vW-1UFdjzeE3l58kmc8eMg&amp;amp;bvm=bv.66699033,d.bGQ]. &lt;br /&gt;
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The tiny (1-2 mm) mango gall flies lay their eggs on the surface of young leaves, buds, shoots and flowers. After hatching, the maggots will eat their way into the plant tissue. This will show as galls (3-4mm) on the leaves. Once mature, they drop to the ground to pupate. The holes that they left behind are susceptible to fungal infections. Infested fruits initially show small (1mm) brownish lesions, which grow as the fruit grows. In young fruits, the exit holes are usually near the point of attachment, on the bottom side. Most infested fruits fall to the ground before ripening. Optimal conditions for reproduction is 26°C and a relative humidity of 70 to 80%. Infestation particularly occurs at bud-burst stage, at fruit set and on tender leaves of new flushes.[http://openagricola.nal.usda.gov/Record/IND92003672]&lt;br /&gt;
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[u]Natural enemies[/u]; Parasitic insects such as Platygaster sp., Eupelmus sp., Tetrastichus sp., Systasis dasyneurae [http://www.cabdirect.org/abstracts/19881115612.html], Pirene sp. and the predator ants Formica rufa (aka red wood ant, native to Europe and North America), Oecophylla longinoda (weaver ant native to West and Central Africa) and Oecophylla smaragdina (native to South-east Asia) and Camponotus sp. (aka carpenter ant, native to forests world wide).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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[u]Baits[/u]; Coriander oil attracts Erosomyia. D. amaramanjarae is attracted by a mixture of glycine, sodium hydroxide, ammonium carbonate and strong ammonia solution. Bordeaux mixture is a repellent. &lt;br /&gt;
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[u]Chemicals[/u]; Various systemic insecticides are equally effective: Dimecron (phosphamidon), Anthio (formothion) and Metasystox (demeton-S-methyl). Most effective is a mixture of phosphamidon (0.03%) and diazinon (0.03%).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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==Mango Moths==&lt;br /&gt;
[[Image:Indarbela_quadrinotata.jpg|thumb|left| Indarbela quadrinotata [http://www.agriplaza.in/orange-protection.htm#indarbela © Agriplaza]]]&lt;br /&gt;
[[Image:Podagrionella_habitus.jpg|thumb|right| Podagrionella [http://www.waspweb.org/Chalcidoidea/Torymidae/Toryminae/Podagrionini/Podagrionella/index.htm © S. van Noort]]]&lt;br /&gt;
[[Image:Indarbela_quadrinotata_2.jpg|thumb|left| Indarbela quadrinotata [http://www.ku.ac.th/e-magazine/september43/bark_feeders/ © KUAC]]]&lt;br /&gt;
[[Image:Eudocima_materna.jpg|thumb|left| Eudocima materna ]]&lt;br /&gt;
[[Image:Mantis_religiosa_5.jpg|thumb|right| Mantid eating large moth [http://www.agefotostock.com/en/Stock-Images/Rights-Managed/K60-589612 © Oriol Cabrero]]]&lt;br /&gt;
[[Image:Eudocima_materna_2.jpg|thumb|left| Eudocima materna [http://www.indianaturewatch.net/displayimage.php?id=285084 © Sivakumar V K]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Eudocima_homaena_2.jpg|thumb|left| Eudocima homaena [http://gaga.biodiv.tw/9702bx/049.htm © GaGa]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Eudocima_homaena_4.jpg|thumb|left| Eudocima homaena [http://blog.xuite.net/mbbrgs/twblog1/137791571-%E9%91%B2%E8%90%BD%E8%91%89%E5%A4%9C%E8%9B%BE++Eudocima+homaena++(Hubner,+1823)]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-l5.php © PathPiva]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella_2.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-1.php © PathPiva]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Orthaga_exvinacea.jpg|thumb|left| Orthaga exvinacea [http://www.nbaii.res.in/insectpests/images/Orthaga-exvinacea7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Orthaga_euadrusalis.jpg|thumb|left| Orthaga euadrusalis [http://www.jpmoth.org/~dmoth/62_Pyralidae/6003_Epipaschiinae/60031001_Orthaga_euadrusalis_1865/Orthaga%20eudrusalis%200208287801.jpg]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_2.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42845]]]&lt;br /&gt;
[[Image:Aleiodes_indiscretus.jpg|thumb|right| Aleiodes indiscretus [http://commons.wikimedia.org/wiki/File:Aleiodes_indiscretus_wasp_parasitizing_gypsy_moth_caterpillar.jpg © Agricultural Research Service]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42848]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_3.jpg|thumb|left| Citripestis eutraphera [http://www.boldsystems.org/index.php/Taxbrowser_Taxonpage?taxid=375866 © ANIC/BIO]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_2.jpg|thumb|left| P. jocosatrix [http://www.nbaii.res.in/insectpests/images/Penicillaria-jocosatrix7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_3.jpg|thumb|left| P. jocosatrix [http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar © DAFF]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix.jpg|thumb|left| P. jocosatrix [http://www.flickr.com/photos/bettaman/3973171117/ ©]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Caterpillar.jpg|thumb|left| Deanolis sublimbalis [http://www.padil.gov.au/pests-and-diseases/pest/main/136263/43328 © S. Eyres]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Moth.jpg|thumb|left| Deanolis sublimbalis [http://www.ces.csiro.au/aicn/name_s/b_1295.htm © DAFF]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Chlumetia_transversa_2.jpg|thumb|left| C. transversa [http://www.nbaii.res.in/insectpests/Chlumetia-transversa.php © NBAII]]]&lt;br /&gt;
[[Image:Chlumetia_transversa.jpg|thumb|left| C. transversa [http://www.inaturalist.org/observations/572035 © T Bonebrake]]]&lt;br /&gt;
[[Image:Goryphus_basilaris.jpg|thumb|right| Goryphus basilaris [http://www.hkwildlife.net/viewthread.php?tid=49119 © Daydream]]]&lt;br /&gt;
[[Image:Parasa_lepida.jpg|thumb|left| P. lepida [http://fanseab.exblog.jp/9707580]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Parasa_lepida_2.jpg|thumb|left| P. lepida [http://www.isan.clubs.chula.ac.th/para_norkhai/?transaction=post_view.php&amp;amp;cat_main=all&amp;amp;id_main=301&amp;amp;star=270&amp;amp;pg=28 © ปิ่นลม]]]&lt;br /&gt;
[[Image:Parus_major.jpg|thumb|right| Parus major [http://en.wikipedia.org/wiki/Parus_major#mediaviewer/File:Parus_major_2_Luc_Viatour.jpg © Luc Viatour / www.Lucnix.be]]]&lt;br /&gt;
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[u]Bark Eating Caterpillar[/u]&lt;br /&gt;
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The Bark Eating Caterpillar (Indarbela quadrinotata and Indarbela tetraonis) aka Mango Bark Feeder has been observed in India, Bangladesh and Sri Lanka. The moth has a wingspan of maximally 2 cm. This wood boring insect may attack a wide variety of trees, including mango, guava, jujube, pomegranate and apple trees. In plantations, infestation generally starts with the onset of premonsoon rains. The main symptom is plastered dark-brown masses on tree trunks and large branches. These webbed masses consist of chewed on wood remainders / frass, excrements and silken thread. Eggs are laid in clusters on the bark of trees. Initially, the newly hatched larvae occur in groups browsing on the bark. Then they migrate and lodge in shoots and buds. As the larvae grow, they start tunneling into the wood, up to 25 cm deep. This tunnel is kept free of frass. The frass and excreta from the tunnels are used for constructing the extended tunnel mouth (sleeve). As the larvae get bigger, they make the tunnel wider. The larvae hide in these tunnels during the day and come out at night to eat the surrounding bark of the tree. The rate of feeding is faster during the hottest months. The larva minimally eats 16 mg / day, at 12.5 degrees Celsius. The maximum is 166 mg/day at 35 degrees Celsius. Similarly, food consumption is maximum at 96% RH and minimum at 56% RH.&lt;br /&gt;
When large patches of bark are eaten away, the tree gets increasingly exposed to various bacteria, fungi etc. And if substantial areas of bark have been eaten, the tree will eventually die. Prolonged water stress decreases the resistance of the host tree. The larvae pupate inside the tunnel and the emerging moth leaves its &amp;quot;pupal skin&amp;quot; at the mouth of the borer hole. The pupa is 1.5 cm long. The larval stage lasts for about 8 months. The pupal period lasts for about 9 days. It has a little more than one generation per year.  [http://www.tropecol.com/pdf/open/PDF_49_1/09%20Shashidharan.pdf]&lt;br /&gt;
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Natural enemies; Two fungi; Aspergillus candidus and Beauveria bassiana have been reported to cause mortality of the Bark Eating Caterpillar in the field. Laboratory trials have indicated 100% larval mortality by these fungi. The Bark Eating Caterpillar is parasitized by Podagrionella indarbelae (&amp;#039;metallic&amp;#039; parasitic wasps), which may be fed the eggs of I. tetraonis.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf] Podagrionella is found in tropical African countries, Senegal, Algeria, Malawi, France, Spain, India, Thailand, Indonesia, The Philippines, Australia&lt;br /&gt;
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Control; prevent overcrowding by sufficient spacing in between trees. One may insert a sharp metallic probe/spike into each tunnel/hole to kill the larvae. One may seal the tunnel entrance using tar or wax. Of the various insecticides screened against this insect, monocrotophos (0.1 %), quinalphos (0.1 %) and fenvalerate (0.08%) gave best results.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf]&lt;br /&gt;
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[u]Fruit-piercing Moths[/u] &lt;br /&gt;
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Eudocima (aka Othreis) are very large moths (up to 10 cm wingspan) with orange abdomen. The most widespread specie is [http://www.waiwiki.org/index.php?title=Eudocima_phalonia Eudocima fullonia] (aka Eudocima phalonia)[http://www.hindawi.com/journals/tswj/2011/753484/] Eudocima moths look like dead leaves when in hiding mode. Until they show their bright orange hindwings with broad black margins and a large black spot in the middle. Mature larvae are 4 to 5 cm long. These moths have been observed in virtually any tropical country, except for the Americas. The adults fly (very well), feed and mate during the night. In tropical countries, they live up to a month. The adults feed on fruit juice by puncturing the fruit. The resulting wound is used by various bugs and diseases to attack the fruit (including mango, banana, grapes, orange, clementine, kiwi, lychee, pineapple, peach, apricot, papaya apple and pear). Eggs are laid singly, or in masses (up to 750 in a cluster, which may be used for rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings]), on any part of the plant. In tropical conditions, eggs hatch within 4 days. Larvae may feed around the clock and mature in 3 weeks, after which they pupate in cocoons of silk-spun leaves. Pupation favors wet conditions and occurs in 12 to 18 days.[http://www.aphis.usda.gov/plant_health/plant_pest_info/pest_detection/downloads/pra/efulloniapra.pdf] Pupation is always in a roomy cell made of living leaves woven together with silk and slightly lined; the pupa is attached to this lining by the cremaster.[http://www.mothsofborneo.com/part-15-16/calpini/calpini_4.php] Temperatures below 16°C during the activity period after dusk prevents feeding, mating and oviposition.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] &lt;br /&gt;
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Their larvae do not feed on mangoes, nor the mango trees. Instead, the larvae feed on very specific Fabaceae and Menispermaceae. Variation in the alkaloids associated with certain menisperm genera (eg quaternary alkaloids in Tinospora[https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2007-969933]) may explain the very specific (or even exclusive) moth–host plant relationships.[http://www.publish.csiro.au/?paper=SB9960227] Eudocima larvae are known to feed extensively on Stephania japonica (Menispermaceae) and its varieties.[http://www.calodema.com/freefiles/412.pdf] In Australia the larvae of Eudocima salaminia feed almost exclusively on the forest vine, Stephania japonica.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] &lt;br /&gt;
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Eudocima materna feeds exclusively on the genus [http://www.waiwiki.org/index.php?title=Eudocima_phalonia#Tinospora Tinospora].[http://onlinelibrary.wiley.com/doi/10.1111/j.1442-9993.1993.tb00471.x/abstract] (widespread in tropical regions [http://link.springer.com/article/10.1007/s00606-004-0293-1#page-1], including Africa [http://informahealthcare.com/doi/abs/10.1080/14756360802333075]). &lt;br /&gt;
Eudocima materna occurs in Ghana (spotted at Vane and Sokode-Etoe, close to Ho in Volta region), Ethiopia, Brazil, Ecuador [http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=140] Nigeria, Pakistan, India, Australia, Costa Rica [http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=40][http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=20][http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=00] and Mexico [http://www.gbif.org/occurrence/search?taxon_key=4687389&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=120]. Eudocima materna and fullonia moths attack citrus and mango [http://www.cabdirect.org/abstracts/19370500524.html], but particularly tomato.[http://www.aapmhe.in/index.php/pmhe/article/view/42]&lt;br /&gt;
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Eudocima homaena may feed on Quisqualis indica.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41], Achyranthes, Cocculus, Cyclea, Menispermum and Tiliacora species.[http://www.en.inforapid.org/index/index.php?search=Cocculus] Eudocima jordani and Eudocima fullonia may be generalist feeders.[http://www.researchgate.net/publication/249439696_Differential_habitat_affinities_of_five_species_of_fruitpiercing_moths_(Lepidoptera_Noctuidae)_in_their_utilization_of_Tinospora_smilacina_Benth._as_a_larval_host_plant_in_north_Queensland]&lt;br /&gt;
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Control; Regularly check the orchard during the night using a flashlight. The adult Eudocima moths have large eyes that glow red when illuminated. Adult [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] may eat large moths such as Fruit-piercing moths. The female Praying mantis lays a few hundred eggs in a cluster (ootheca) attached to an object like a branch or trunk. Large species of mantids may have a length of up to 12 cm. Their colour is usually adapted to the environment they naturally live in. They have about 2 generations per year. Young mantids look like adults, but don&amp;#039;t have wings. Adult females often have no wings either, or reduced wings.&lt;br /&gt;
Eudocima are parasitized by Euplectrus [http://link.springer.com/article/10.1023/B:BICO.0000036439.74117.2f#page-1][http://www.aapmhe.in/index.php/pmhe/article/view/42] and Trichogramma.[http://images.peabody.yale.edu/lepsoc/jls/2010s/2011/2011-65-1-053.pdf]&lt;br /&gt;
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[u]Honeydew Moth[/u]&lt;br /&gt;
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The Honeydew Moth (Cryptoblabes gnidiella aka Albinia casazzar aka Albinia wockiana) aka Christmasberry Moth survives only in warm climates (all over the world). It attacks a large variety of crops, including mangoes, banana, oranges, clementines, grapes, loquats, pomegranates, avocado, coffee, maize and rice. The caterpillars (1 cm long) mainly feed superficially on fruits, but may also feed on leaves, flower, shoots and bark. The newly hatched larvae and the adults, however, solely feed on honeydew. The adults are active during the night. Females mate 1 to 4 times during their entire life. One Honeydew Moth may lay up to 100 eggs within 2 days after mating, on fruit or leaves (averagely 150 in total, during lifetime). These eggs hatch within one week (or longer, in colder regions). It has several generations per year, depending on the climate. Adult moths are good flyers (1 to 2 cm wingspan) and may rapidly spread to poorly maintained orchards (infested with honeydew).&lt;br /&gt;
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Natural enemies; Scolothrips sexmaculatus and Orius spp. prey on the eggs and early-instar larvae. The Honeydew Moth is parasitized by Phanerotoma sp.[http://www.cabdirect.org/abstracts/19740514736.html] and Trichogramma Platneri (both parasitoid wasps).&lt;br /&gt;
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Trichogramma are extremely tiny wasps (&amp;lt; 0.5 mm). They insert their eggs into the eggs of the moth. The larvae feed, grow and pupate inside the host egg, which converts the colour of the host egg from white to black. The emerging wasp eats its way out and is ready to mate. The eggs from unmated females will produce only males, whereas the eggs from mated females will produce both males and females. Adult Trichogramma feed on nectar from flowers. One female wasp may parasitize 50 moth eggs during her life. The optimimum conditions for Trichogramma are 20 to 27 degrees Celsius and 60% RH. Most insecticides kill Trichogramma.&lt;br /&gt;
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Control; This moth is attracted by honeydew secreted by aphids, scale insects and other honeydew secreting bugs. It is therefore most effectively controlled by controlling all honeydew secreting bugs. Its instars are highly susceptible to Bacillus thuringiensis.&lt;br /&gt;
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[u]Leaf Webber[/u]&lt;br /&gt;
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These brown moths (Orthaga euadrusalis and Orthaga exvinacea) are a minor mango (and Cashew nut) pest. They have been observed in India and Indonesia. Females mate only one time during their whole life.[http://www.ncbi.nlm.nih.gov/pubmed/20136014] The leaf webber lays eggs on young leaves and buds and creates a web around pods, leaves and flowers for its larvae to pupate in. Such a webbed cluster may contain several larvae. The Leaf Webber causes relatively very little damage, as the larvae merely scrape and eat from the surface of the leaves.&lt;br /&gt;
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Control: Application of Serratia marcescens (a bacterium), Aspergillus flavus (a fungus) and Beauveria bassiana (a fungus) are all very effective.[http://www.cabdirect.org/abstracts/19810586732.html] The leaf webber is parasitized by Brachymeria lasus, Hormius sp., Pediobius bruchicida and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html] and also by Trichogramma chilonis and Trichogramma pretiosum.[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=37933]&lt;br /&gt;
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[u]Mango Fruit Borer[/u];&lt;br /&gt;
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The Mango fruit borer (Citripestis eutraphera; a moth) has been observed in Indonesia, India and Australia. Its lays its eggs (&amp;lt; 1 mm) on fruit or stalk. The larvae feed on mango pulp (and cashews) and cause premature fruit drop, mainly in young fruit. The larvae hatch in 2 days. They are initially white, then red-violet when young, turning to dark blue as they grow. Hatched larvae initially feed on the peel of the fruit. Then they bore into into the fruit and feed for 12 to 15 days before they pupate in the fallen fruit, or in the soil. Often there is more than one larva in a fruit with a total of 15 recorded in one fruit.[http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42846].&lt;br /&gt;
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Control: Its natural enemies are parasitoid wasps (Aleiodes sp. and Euplectrus sp.) and a parasitoid fly (Blepharella lateralis).&lt;br /&gt;
[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=9416] To protect the stems, cover them with a cloth or Jute and paste charcoal over it. Fostoxin tablets can also be placed and sealed in the holes made by the borers.&lt;br /&gt;
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[u]Mango Shoot Borer[/u]&lt;br /&gt;
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Mango Shoot Borer (Penicillaria jocosatrix) or Velvet mango slug; P. jocosatrix (wingspan 2 - 2.5 cm) has been observed in Indonesia, The Philippines, Malaysia, Vietnam, Guam, Hawaii, Thailand and Australia. Its larvae; the caterpillar of this moth (up to 3 cm long) causes damage to shoots, stems, inflorescence, flowers and fruits of the mango tree. Pestalotiopsis anacardiacearum sp. nov. (fungal leaf rot) is found on dead mango leaves associated with P. jocosatrix (the Mango Shoot Borer).[http://biotaxa.org/Phytotaxa/article/view/phytotaxa.99.2.1] Eggs hatch in 3-5 days. Larval development takes 8-10 days. Mature larvae pupate in the soil and the moth emerges 16-20 days later.[http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar]&lt;br /&gt;
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Natural enemies: Euplectrus sp. (a parasitoid wasp) and Blepharella lateralis (a parasitoid fly) may effectively decrease Mango shoot borer infestation [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], and are also effective against the Mango fruit borer.&lt;br /&gt;
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[u]Mango Seed Borer[/u]&lt;br /&gt;
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The Mango Seed Borer (Deanolis sublimbalis aka Autocharis albizonalis) is the Red banded mango caterpillar. It is a mango pest in Australia, Burma, The Philippines, Malaysia, Vietnam, China, Indonesia and Papua New Guinea. Severe infestation may cause up to 50% yield reduction. This grey snout moth (wingspan 2 cm) lays its eggs on the top of the mango. Its larvae (one or more per fruit) develop within the fruit in 2 to 3 weeks. Initially they feed on the pulp, and then on the seed. The fruit will split, rot and drop. The mature larvae will pupate in the soil.&lt;br /&gt;
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Natural enemies: Rychium attrisimum are wasps that feed on the larvae of the Mano Seed Borer as they leave the fruit.[http://www.planthealthaustralia.com.au/wp-content/uploads/2013/03/Red-banded-mango-caterpillar-CP.pdf] Trichogramma chilonis and Trichogramma chilotreae are tiny parasitoid wasps that parasitize the eggs of the Mango Seed Borer and hundreds of other moths. They are so small that with the naked eye you cannot see what they are. They have a wingspan of 0.5 mm. They live about 2 weeks, and may lay several eggs in a single moth egg.&lt;br /&gt;
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Chemicals: If you have no other option, Deltamethrin and Cyfluthrin are most effective.&lt;br /&gt;
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[u]Mango Tip Borer[/u]&lt;br /&gt;
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Mango Tip / Shoot / Bark / Trunk / Twig Borer (Chlumetia transversa) aka aka Shoot Boring Caterpillar is a serious mango (and litchi) pest. The major symptom is drying of the tip of young shoots. Female moths lay their eggs on tender leaves. Newly hatched larvae bore into these leaves. As they grow they bore into young shoots near the growing point, and subsequently tunnel down. The leaves of affected shoots will droop. It has four generations each year, and in colder regions overwinters as pupae in young branches and bark. Generations are overlapped.[http://europepmc.org/abstract/CBA/367089] Full grown caterpillars are 2 to 2.5 cm long.&lt;br /&gt;
C. transversa has been observed in Indonesia, The Philippines, Malaysia, Thailand, Taiwan, China, India, Pakistan, Sri Lanka and Australia. &lt;br /&gt;
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The Mango Tip Borer is parasitised by Bracon greeni, Meteorus sp. and Goryphus sp [http://www.cabdirect.org/abstracts/19810586735.html], which are all parasitoid wasps. Its larvae are also parasitized by the fly Megaselia chlumetiae sp. nov. Its larvae enter the host and develop by consuming the host&amp;#039;s internal tissues. Pupariation normally took place within the host&amp;#039;s empty skin.[http://www.cabdirect.org/abstracts/19921163681.html]&lt;br /&gt;
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Control; If you have no other option, spraying 2.5% deltamethrin EC for three times may be most effective (87% control efficiency) and the least harmful to the tree. Four insecticides tested; 80% dichlorvos EC(dilution of 1250), 47.5% chlorpyrifos EC(dilution of 1000), 2.5% deltamethrin EC (dilution of 2500) and 2% abamectin EC(dilution of 2000) were applied on mango trees during 2 months. Second best was 2% abamectin EC at a dilution of 2000. The other two insecticides were less effective, which might be due to the development of resistance in pests against these insecticides on account of longer repeated applications.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GXNY200910010.htm]&lt;br /&gt;
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[u]Nettle Caterpillar[/u]&lt;br /&gt;
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The Nettle Caterpillar, aka the Blue-striped Nettle Grub (Parasa lepida aka Latoia lepida; 2 to 3 cm long) has been observed in China, Pakistan, India, Sri Lanka, Bangladesh, Vietnam, Thailand, Malaysia, Japan and especially Indonesia. Parasa lepida may infest various fruit trees, including mango, cherry, persimmon, Indian almond and rose apple, and also coffee, tea, cocoa and palms, with a preference for coconut trees. The caterpillar of this species have spines containing a poison that causes serious irritation and inflammation in humans upon contact. It feeds on the leaves and shoots of the mango tree. Initially, defoliation will be localized, affecting only one or a few trees. Thus the infestation is easily identified. Subsequently, as new generations of this pest emerge, the infested area may rapidly expand. The female moth lays eggs on mango leaves. The cocoons are laid side by side. The cocoons are so hard and stout that they remain on the trunks for 5 to 6 years after spinning. Cocoons from which adults have successfully emerged have clear emergence holes. Newly hatched caterpillars will feed on the underside of the epidermis, stripping it off the leaflets. They often begin where the eggs were laid; at the tip. Then they eat the edges of the leaflet and eat large areas of the lamina. When they have finished developing, the whole leaflet will have been consumed systematically from tip to base, leaving only the midrib. On this midrib, the notched indentations are the only visible remainders left by the caterpillars. [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=38935]&lt;br /&gt;
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Parasa lepida is predated on by Agriosphodrus dohrni (The Assassin Bug, native to China, India, Indonesia). This 2 cm long Assasin Bug may be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
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The great tit (Parus major) is a predator of Parasa lepida cocoons in Japan. The most effective parasites are Apanteles parasae (a parasitoid wasp) and Chaetexorista javana (a parasitoid fly).[http://www.cabdirect.org/abstracts/19820596771.html]&lt;br /&gt;
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Salt spray from the sea and the resulting plant stress (considerable damage) may negatively affect the Parasa lepida moth.[http://www.bioone.org/doi/pdf/10.3956/0031-0603-83.3.193]&lt;br /&gt;
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==Mango Scales==&lt;br /&gt;
[[Image:Coccus_viridis.jpg|thumb|left| Coccus viridis [http://www.insectimages.org/browse/detail.cfm?imgnum=5385208 © J.W. Lotz]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| M. signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
[[Image:Coccus_hesperidum.jpg|thumb|left| Coccus hesperidum [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/09Cocc_hesperidum/4hesperidumHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Ceroplastes_floridensis.jpg|thumb|left| Ceroplastes floridensis [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/03Cero_floridensis/4floridensisHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Chilocorus_kuwanae.jpg|thumb|right| C. kuwanae [http://animal.memozee.com/view.php?tid=2&amp;amp;did=10472 © Jinsuk Kim]]]&lt;br /&gt;
[[Image:Aulacaspis_tubercularis.jpg|thumb|left| Aulacaspis tubercularis[http://gaga.biodiv.tw/new23/9409/076.htm]]]&lt;br /&gt;
[[Image:Azya_orbigera.jpg|thumb|right| A. orbigera [http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php © E Arcaya]]]&lt;br /&gt;
[[Image:Drosicha_mangiferae.jpg|thumb|left| D. mangiferae [http://www.jugantor.com/last-page/2014/04/19/89802 ©]]]&lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri.jpg|thumb|right| C. montrouzieri[http://www.ozanimals.com/Insect/Mealybug-Ladybird/Cryptolaemus/montrouzieri.html ©]]]&lt;br /&gt;
[[Image:Rastrococcus_iceryoides.jpg|thumb|left| R. iceryoides [http://www.nbaii.res.in/insectpests/Rastrococcus-iceryoides.php © NBAII]]] &lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri_larva.jpg|thumb|right| C. montrouzieri larva [http://www.insectimages.org/ © J.W. Lotz, FDACS Bugwood.org]]]&lt;br /&gt;
[[Image:Paracoccus_marginatus.jpg|thumb|left| Papaya mealybug [http://www.ablturismo.com/avispa-enviada-por-correo-salva-cultivos-en-la-india/ ©]]]&lt;br /&gt;
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The main mango scales are:&lt;br /&gt;
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* Coccids ; Coccus viridus (soft green scales) and Coccus hesperidum (soft brown scales)&lt;br /&gt;
* wax scales ; Ceroplastes spp.&lt;br /&gt;
* armoured scales ; Aulacaspis tubercularis, Chloropulvinaria polygonata (the Mango Green Shield Scale) and Aspidiotus destructor.&lt;br /&gt;
* Mealybugs (pseudococcidae) ; Drosicha mangiferae, Rastrococcus iceryoides (&amp;quot;Mango mealybugs&amp;quot;) and Paracoccus marginatus (&amp;quot;Papaya mealybug&amp;quot;, which is also a mango pest)&lt;br /&gt;
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Mango scales suck the sap from the leaves, and as a result, the leaves start drying. This may cause branches to die, blemished fruits and premature dropping. Scales are small insects (1 to 7 mm), generally immobile; as if shells are glued to the plant. Only the newly hatched crawlers (emerging from under a big scale) move to their feeding site. Soft scales produce honeydew, which may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould]. Armoured scales don&amp;#039;t excrete honeydew. The papaya mealybug has been observed in Mexico, Florida, Antigua, Cuba, Dominican Republic, Guadeloupe, Haiti, St Kitts, Martinique, Puerto Rico, St Martin, St Barthélémy, Virgin Islands.[http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=6&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEIQFjAF&amp;amp;url=http%3A%2F%2Fwww.cabi.org%2FISC%2FFullTextPDF%2F2013%2F20133231104.pdf&amp;amp;ei=VeZ8U_eFNaqX1AXh_YHYDA&amp;amp;usg=AFQjCNGWK-GGi3IRhAfSNBg6nah3XhoKWw&amp;amp;bvm=bv.67229260,d.d2k]&lt;br /&gt;
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[u]Natural enemies[/u]; Natural enemies of scales include parasitic wasps, ladybird beetles and lacewings, which are killed by broad-spectrum pesticides. Because of their honeydew secretion, scales may be protected / farmed by specific ants (eg arboreal ants (Azteca instabilis) farming soft green scales).[http://www.ncbi.nlm.nih.gov/pubmed/18559179] Some fungal pathogens effect this symbiotic interaction.[http://www.ncbi.nlm.nih.gov/pubmed/23905728] Parasitoid insects use the honeydew secreted by coccids (and aphids etc) as an infochemical to locate their hosts.[http://www.ncbi.nlm.nih.gov/pubmed/15112724] R. iceryoides is parasitized by Dinocarsis sp., Microterys flavus, Metastenus concinnus and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
Of the parasitoids Acerophagus papayae and Anagyrus loecki, A. papayae is the most effective in decimating the papaya mealybug. (Meyerdirk)&lt;br /&gt;
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[u]Lacewings[/u]; Most lacewings (also) feed on plant material, such as pollen. Particularly green lacewings such as Mallada boninensis, Mallada astur and Mallada signata are mainly predatory and very effective. They are relatively small (length up to 1.5 cm, including wings). Adults are active during the night, and may be attracted by flashlight. Eggs are attached to leaves and hatch within 4 days. The larval period lasts 12 days, and the pupal period lasts about 9 days. Adults live just one month. One may commercially rear millions of green lacewings for biological control of mango pests.&lt;br /&gt;
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[u]Cryptolaemus montrouzieri[/u] (aka Mealybug Ladybird) is a (4 mm long) predatory ladybird that eats mealybugs, soft scales (Coccidae) and armoured scales (Diaspididae). Originally from Australia, introduced in Florida and commercially available as a mealybug control agent. It needs warm, humid weather. Survival temperature range is 15-35°C. Life cycle on average is 30 days under optimum conditions: 23 °C and 60-70% humidity. Optimal temperature for adults is 28°C. They are active between 16°C and 33°C, and 70 to 80% RH. Longevity of females is 70 days max. It produces 4 generations per year. Development is accelerated at higher temperatures. At 25°C it may lay a total of 200 to 700 eggs (av. 400), at the rate of averagely 9 eggs per day. They preferrably lay their eggs in the egg mass of mealybugs. The eggs hatch in 5 to 6 days. There are 4 larval stages, and the entire larval period lasts about 12 to 17 days. At pupation stage they are about 13 mm long. Adults emerge after 7 to 10 days. These beetles prefer high concentrations of mealybugs to predate on, and will fly away when concentrations of mealybugs are lower.[http://www.arabscientist.org/english/page/11/] During its larval development, one C. montrouzieri may eat 2400 eggs of C. polygonata.[http://www.cabdirect.org/abstracts/19981112241.html] Unfortunately, the filaments produced by its larvae look very similar to mealybugs. Initially release 1 or 2 new active beetles per square meter in the morning, before it gets hot. Subsequently regularly release 2 to 3 beetles per tree. The beetles will be killed by pesticides, but tolerate copper-fungicides.&lt;br /&gt;
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[u]Chilocorus kuwanae[/u]; This ladybird beetle is a primary predator of wax scales (Ceroplastes sp.). This ladybird beetle is attracted by (spraying) methyl jasmonate, mainly due to the terpenoid compound alpha-pinene [http://www.ncbi.nlm.nih.gov/pubmed/19825299]&lt;br /&gt;
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[u]Microweisea coccidivora[/u]; a tiny (1mm long) predatory ladybird beetle from Florida and South Carolina (USA) that feeds particularly on armoured scales.&lt;br /&gt;
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[u]Azya orbigera[/u]; The larvae of this ladybird beetle predate on Coccus viridis. These larvae are relatively immune to ant attacks (ants that farm coccids), due to their sticky waxy filaments. Also, the presence of ants reduces A. orbigera larvae getting parasitized.[http://www.ncbi.nlm.nih.gov/pubmed/18348805] A. orbigera has been observed in Colombia, Guyana, Venezuela, Bélize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, México, USA, Trinidad and the West Indies.[http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php]&lt;br /&gt;
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[u]Sumnius cardoni[/u] (a predatory ladybird) predates on nymphs of D. mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19810586735.html] &lt;br /&gt;
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[u]Coccodiplosis sp[/u] (a parasitic gall midge)[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9], Cybocephalus sp. (a predatory beetle) and Scymnus coccivora (a predatory ladybird) predate on R. iceryoides (mealybug). &lt;br /&gt;
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[u]Cryptochetum sp.[/u] (parasitic flies) parasitize the 1st- and 2nd-instar nymphs of the Drosicha mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
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[u]Coccophagus scutellaris[/u] parasitizes Coccus hesperidum by laying eggs in its gut.[http://www.ncbi.nlm.nih.gov/pubmed/747455] When parasited and parasiteless hosts are available, the female of Coccophagus deposits more eggs on the latter.[http://www.ncbi.nlm.nih.gov/pubmed/7283545]&lt;br /&gt;
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[u]Thomsonisca pakistanensis[/u] is a parasitic wasp that most commonly parasitizes Aspidiotus destructor. A. destructor is also parasitized by Aneristus ceroplastae (tiny parasytic wasp), Comperiella bifasciata (parasytic wasp also effective against yellow scales; Aonidiella citrina[http://www.publish.csiro.au/paper/ZO9710053.htm]), Chartocerus sp. and Chrysonotomyia sp. (both also parasytic wasps) [http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
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[u]Aphytis sp[/u]; This tiny (2 to 3 mm long) parasytic wasp parasitizes Aulacaspis tubercularis (up to 46% parasitism in a test).[http://www.actahort.org/books/509/509_96.htm]&lt;br /&gt;
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[u]Lecanicillium lecanii[/u]; This fungus kills various coccids, including Coccus hesperidum.[http://www.ncbi.nlm.nih.gov/pubmed/20863711] Also Ceroplastes sp. may be killed by Lecanicillium lecanii. Adding body materials of life coccids to a multi-generation culture (medium), significantly keeps the vigor and higher virulence of this fungus.[http://www.ncbi.nlm.nih.gov/pubmed/20387464]&lt;br /&gt;
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[u]Control[/u]: Collect the affected leaves and burn them. Spraying chlorogenic acid (a phenol naturally present in coffee beans) stimulates locomotory activity of the green scale Coccus viridis, thus minimizing their feeding. [http://www.ncbi.nlm.nih.gov/pubmed/20857759]&lt;br /&gt;
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If you have no other option: Use Metasystox 25% EC at the rate of 0.3 L in 450 L water or Fotidal 50 EC at the rate of 0.5 L in 450 L water / acre. Or spray mineral oil at low concentrations (not damaging the trees) after fruit picking, but not during flowering. Don&amp;#039;t spray during droughts or excessive heat.&lt;br /&gt;
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==Weevils==&lt;br /&gt;
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[u]The Mango Stone Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_mangiferae.jpg|thumb|left| Mango Stone Weevil [http://en.wikipedia.org/wiki/Sternochetus_mangiferae#mediaviewer/File:Mango_seed_weevil_2.jpg © P.Jeganathan]]]&lt;br /&gt;
[[Image:Oecophylla_smaragdina.jpg|thumb|right| Stone Weevil captured by O. smaragdina [http://www.cabi.org/isc/datasheet/16434 © Renkang Peng]]]&lt;br /&gt;
[[Image:Mantis_religiosa.jpg|thumb|right| Praying mantis [http://www.statesymbolsusa.org/Connecticut/insect_praying_mantis.html © Andy Williams / CritterZone]]]&lt;br /&gt;
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The Stone Weevil (Sternochetus/Curculio/Rhynchaenus/Cryptorhynchus mangiferae) aka Mango Seed / Nut Weevil is a short weevil; 7 mm to 1 cm long and 4 mm wide. It possesses a tough exoskeleton. Weevils are a type of beetle. The scales of the Stone Weevil may be of various colours; shades of black, grey, red or yellow. The Stone Weevil has been observed in many mango-producing countries all over the world (including Ghana, Nigeria, Kenya, Mozambique, Tanzania, Uganda, Zambia, India, Bangladesh, Indonesia, Thailand, Malaysia, Australia, USA)[http://www.cabi.org/isc/datasheet/16434]. Adult weevils can hardly fly, and this pest spreads by fruit transports. At dusk and during the night, the adults feed on leaves and young shoots. The female lays her eggs on and just under the surface of the peel of young (about 3 cm long) mangoes. Each female can lay up to 15 eggs per day and may lay several eggs on one fruit. The Stone Weevil prefers the sweetest (high-sugar) varieties of mango. After laying an egg, the weevil will make an incision in the fruit to let the sap from the fruit cover the egg, and to make it stick to the fruit. The amber-coloured sap will harden out, and remain as a protective resin mark over the egg. Newly emerged grubs are white and slender, and have no legs. They bore their way through the pulp towards the seed, within 2 days. Larvae and pupae develop inside the fruit. Pupation occurs in the seed. No external symptoms of attack are readily visible on infested fruits. Once matured (which takes up to 2 months), they eat their way out. About 25% of adult the weevils over-winter in the seed. They can live 2 years. One generation is produced each year.&lt;br /&gt;
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Natural enemies; The Stone Weevil is effectively predated on by Weaver ants such as Oecophylla smaragdina and Oecophylla longinoda.[http://iiste.org/Journals/index.php/JBAH/article/view/12284] Weaver ants, however, may also farm (for the produced honeydew) and protect aphids and various mango scales against their natural enemies. &lt;br /&gt;
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Weevils are also predated on by [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids], which may eat anything they can catch, including flies, beetles and moths. Adults readily mate in captivity and have one generation per season. Rearing mantids requires rearing of other insects, such as aphids and drosophilae (for nymphs), tephritids (for young mantids) and moths (for adult mantids) in large quantities. Females lay their eggs in a sticky cluster of several cm long. Nymphs (very tiny praying mantids) emerge from eggs. The developing nymphs need to be separated and fed aphids/drosophilae, or they will become cannibalistic.&lt;br /&gt;
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Control; Collect and destroy all fallen fruits, seeds, leaves, twigs etc. If you have no other option, spraying Fenthion (0.01%) on the trees at the time of oviposition is found to be effective. Fenthion, however, is extremely toxic to beneficial insects [http://www.actahort.org/books/509/509_96.htm], so that applying Fenthion may lead to secondary infestation of Mealybugs. A single spray of tiametoksam (Actara™ SC 240g/ℓ a.i.) at the roots during flower set may be effective for seasonal control.[http://etd.uovs.ac.za/ETD-db/theses/available/etd-09182009-083002/unrestricted/LouwCE.pdf] In Alphonso and Bagan Pali mangoes, a single spray (at dusk) of Monocrotophos 36EC 1ml per 1 litre of water was found to be very effective (97.5% to 100% mortality) [http://issuu.com/kisanadmin/docs/mango1/16] In a laboratory test, Beauveria bassiana was somewhat effective (30% mortality in 14 days), but in an orchard setting there was no effect at all.[http://www.cabdirect.org/abstracts/19971101160.html]&lt;br /&gt;
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[u]The Mango Pulp Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_frigidus.jpg|thumb|left| Mango Pulp Weevil [http://www.thaibugs.com/?page_id=284 © Thaibugs]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
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The Mango Pulp Weevil (Sternochetus frigidus or Cryptorrhynchus gravis) has been observed in Bangladesh, India, Indonesia, Malaysia, Myanmar,&lt;br /&gt;
Pakistan, Papua New Guinea, Philippines, Singapore and Thailand. This female weevil lays eggs (25-60 eggs / week) on mango fruits (minimum 6 cm diameter). Newly hatched larvae tunnel into the fruit pulp. The larvae form a chamber (constructed of frass) next to the seed, from which they eat their way through the pulp. Pupation occurs within these chambers. Matured weevils leave the ripe fruit through an exit hole in the peel. Prior to this exit, nothing shows that the fruit is infected. They are fully matured after 6 weeks; able to mate, repeatedly. One complete life cycle varies from 34-35 days. More than half of adult weevils hibernate in seeds. Mango Pulp Weevils are very poor flyers (maximum 90 cm horizontally).[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Thrips==&lt;br /&gt;
[[Image:Frankliniella_occidentalis.jpg|thumb|left| F. occidentalis [http://conabio.inaturalist.org/taxa/243761-Frankliniella © Th0th]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Thrips, or thunderflies, thunderbugs, storm flies, thunderblights, storm bugs, corn flies or corn lice may feed on many plants. Frankliniella bispinosa, Frankliniella kelliae (Florida, Caribbeans) and Frankliniella occidentalis (California, Israel) also feed on mango. They feed on young leaves, shoots and flowers, causing discoloration and deformation.&lt;br /&gt;
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Thrips are tiny (1 mm long on average; varying from 0.5 mm to 1.4 cm). They are not good flyers, but may readily be carried by the wind. Given the right conditions, they may rapidly multiply and form large swarms. To inspect trees for thrips infestation, just hold a bucket and shake a branch, and some thrips will fall off the infested branch into the bucket.&lt;br /&gt;
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Control: Natural enemies of thrips are Green lacewings (eg Mallada boninensis, Mallada signata), Orius insidiosus, Anystis agilis and Hypoaspis aculifer. Biological insecticides such as Beauveria bassiana and Verticillium lecanii may be effective.&lt;br /&gt;
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[u]Orius insidiosus[/u] is a 3 mm long pirate bug (aka insidious flower bug). They feed on thrips, mites, small caterpillars and various insect (including aphids&amp;#039;) eggs and larvae, and also pollen. They prefer eating thrips over aphids.[http://www.ncbi.nlm.nih.gov/pubmed/18845007] Orius may kill several thrips per day, beyond their need for nutrients. Adults fly well, and may readily disperse, looking for prey. O. insidiosus may be mass reared on [http://www.waiwiki.org/index.php?title=Armyworm armyworms]. They may occasionally sting people, which may hurt, but is completely harmless. Eggs are laid and embedded in the plant tissue of the fruit, stem, leave stalks and big veins at the underside of leaves. From eggs to adults, Orius goes through 5 larval stages. This takes about 16 to 18 days at 25°C. They are predatory at each stage (except as pupae). Adults live 3 to 4 weeks. As Orius albidipennis lays more eggs and has a shorter lifecycle, it may be a better biocontrol agent than Orius insidiosus.[http://eurekamag.com/research/030/621/030621437.php] Release at 2 to 3 Orius per square meter.&lt;br /&gt;
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==Whiteflies==&lt;br /&gt;
[[Image:Trialeurodes_vaporariorum.jpg|thumb|left| Trialeurodes vaporariorum [http://en.wikipedia.org/wiki/File:Weisse-Fliege.jpg © Gaucho]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| Orius insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Paraleyrodes_bondar.jpg|thumb|left| Paraleyrodes bondar [http://manateeornprod.wordpress.com/2012/02/22/new-whitefly-in-florida/ © UF/IFAS]]]&lt;br /&gt;
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Whiteflies (incl. Aleurodicus rugioperculatus, Aleurodicus dispersus, Aleurothrixus floccosus, Aleyrodes proletella, Bemisia tabaci, Paraleyrodes bondar, Siphoninus phillyreae, Trialeurodes vaporariorum) attack various crops, including mango, orange, tomato and watermelon. &lt;br /&gt;
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Whiteflies suck the saps from leaves, causing direct damage. They are also vectors of various diseases, including viruses. Additionally they may produce a lot of honeydew, which may lead to sooty mould. The density of the whitefly is positively correlated with maximum temperature and negatively correlated with relative humidity.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Natural enemies[/u]; Various animals predate on whiteflies, including green lacewings, Orius insidiosus and ladybird beetles. The minute parasitic wasps Encarsia guadeloupae and Encarsia haitiensi very effectively cause reduction in the population of Aleurodicus despersus.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Control[/u]&lt;br /&gt;
Dissolve 1 kg of sugar per 1 L of water. Poor in plastic transparent bottle. Drill holes in the upper part (above water level); small enough for whiteflies to enter, but not for bees. Put the lid on. Hang 2 to 3 bottles in each tree at flower set. Renew weekly.&lt;br /&gt;
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=Integrated Pest Management=&lt;br /&gt;
Integrated Pest Management (IPM) integrates proper orchard managment (such as sufficient spacing of trees, pruning off overlapping branches, the use of bait traps and removing affected fruits and plant parts) and the use of a variety of anti-bugs (natural enemies of mango bugs), so that they are complementary and sufficiently reduce all pest populations, minimizing the use of pesticides.&lt;br /&gt;
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Below is an example of the complementary use of anti-bugs, for both predation and parasitism.&lt;br /&gt;
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Preliminary requirements: A sealed, ventilated room. Constant supply of mangoes, or grapes, kiwi, lychee, pineapple, peach, apricot, papaya, apple or pear, to feed and rear Drosophilae (2-4mm long &amp;#039;fruit flies&amp;#039;), Tephritids (4-7 mm long &amp;#039;true&amp;#039; fruit flies) and Eudocima sp. (fruit piercing moths; up to 10 cm wingspan). One section needs daylight exposure, for growing Fabacae / Menispermacae for the Eudocima sp. to lay their eggs, and for their larvae to feed on the leaves. This sealed room should rather be too large than too small to prevent a lack of food for the reared predators. Any surplus of eggs and/or larvae may be sold as fish feed.&lt;br /&gt;
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* Rearing [http://www.waiwiki.org/index.php?title=Praying_mantis Praying Mantids] to predate on moths, caterpillars, weevils and young Longicorn beetles by adults, and on fruit flies, hoppers and midgets by young mantids. These mantids may be reared on fruit flies  (Drosophila and Tephretids) and specific moths. Young mantids should be separated after hatching, in boxes. Nymphs should be fed Drosophilae. Young mantids should be fed Tephretids. Adult mantids are fed Eudocima moths or Armyworm moths.&lt;br /&gt;
* Rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings] (Mallada signata, Mallada boninensis) to predate on aphids, scales, hoppers and thrips in the field. These lacewings may be reared on Eudocima sp. eggs collected from the Fabacae / Menispermacae in the sealed room, or on Armyworm eggs. &lt;br /&gt;
* Rearing the parasitic wasps Fopius arisanus and/or Diachasmimorpha longicaudata to parasitize Tephritids (fruit flies). Tephritid eggs should be collected from fruits in the sealed room. To prevent the fruit fly eggs from drying out, the eggs should be kept on a moist substrate. To prevent molding, a constant air current should be provided during the time required for parasitoid development.&lt;br /&gt;
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The second phase may be more labour intensive. To rear Tetrastichus sp. and Euplectrus sp. or Trichogramma sp. for the purpose of parasitizing scales, moths, midges and longicorn beetles, one most collect eggs from all species in the field. Instead, one may also rear subject pests.&lt;br /&gt;
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As an alternative for green lacewings, one may use the combination of Cryptolaemus montrouzieri (mass reared on Plancoccus citri on potatoes or squash [http://www.arabscientist.org/english/page/11/], for predating on scales) and Orius insidiosus (mass reared on [http://www.waiwiki.org/index.php?title=Armyworm Armyworms] (which are best reared on Bermudagrass), for predating on aphids, thrips and small caterpillars). This allowes for a more targeted approach (if there is only 1 pest, eg scales). This may alsobe a better combination (together with Praying mantids) because adult Praying mantids prefer to predate on lacewings (15 mm long) over the much smaller C. montrouzieri (4 mm) and O. orius (3 mm).&lt;br /&gt;
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=Diseases=&lt;br /&gt;
Bugs cause damage to the mango tree, which makes the tree more susceptible to disease. Many bugs are also disease vectors; they transport the viruses, fungi etc. to the mango tree. Successfully combatting mango bugs, is therefore key to prevention and control of mango diseases. Using chemicals to fight mango diseases and/or bugs, however, may actually result in killing natural enemies of the bugs that spread disease.&lt;br /&gt;
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==Anthracnose==&lt;br /&gt;
[[Image:Anthracnose.jpg|thumb|left| Anthracnose [http://www.indiancropdiseases.com/mango.aspx]]]&lt;br /&gt;
[[Image:Anthracnose-2.jpg|thumb|right| Anthracnose [http://hawaiiplantdisease.net/cpg/displayimage.php?pid=397 © Nelson]]]&lt;br /&gt;
Anthracnose is caused by the fungi Colletotrichum acutatum [http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2003.93.5.579] and Glomerella cingulata [http://www.idosi.org/wjas/wjas4(6)/8.pdf][http://www.jofamericanscience.org/journals/am-sci/am0608/46_3350am0608_361_367.pdf]. It particularly affects young shoots, flowers and fruits. The disease is stimulated by high humidity, frequent rains or mists, and a temperature range of 24 to 32°C. The results of this disease are black spots on panicles, leaf spot, blossom blight, withered tip, twig blight and fruit rot. Particulary young and tender shoots and foliage are affected, causing die back of young branches. Wounded twigs may also be infected and may sometimes die off. Fruits develop black spots and rotting and young fruits may shrivel and prematurely drop. If the entire inflorescence is destroyed, no fruits will set. &lt;br /&gt;
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[u]Where?[/u] It widely occurs in the orchard and in storage, Anthracnose has been reported in Argentina, Florida, Hawaii, India, Pakistan, Philippines, Sri Lanka, South Africa and Trinidad.&lt;br /&gt;
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[u]Control[/u]: Burn all fallen leaves. Prune affected twigs and burn them. Apply Bordeaux paste on cut ends. To prevent blossom infection, spray twice at 15 days interval during flowering with Carbendazirn (Bavistin 0.1%). To prevent foliar infection, spray copper fungicides (0.3%) twice a week. Apply a teaspoon of dish detergent per spray load to make the copper sulfate stick. Prevent the copper sulfate from dripping on the ground to prevent contamination of the soil and the roots of the tree.&lt;br /&gt;
Picked mangoes should be submerged for 15 minutes in hot water (52°C) with Carbendazim (0.1%). One may also dip the mangoes in 500 ppm Benomyl and 900 ppm Thiobendazole. C. acutatum is also effectively inhibited by the synthetic strigolactone GR24.[http://www.ncbi.nlm.nih.gov/pubmed/21688170]&lt;br /&gt;
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==Bacterial Canker==&lt;br /&gt;
Bacterial black spot (bacterial canker) is a bacterial disease caused by Xanthomonas campestris pv. mangiferaeindicae. It affects many varieties. Initially, irregular small water soaked lesions will appear on the leaves. These will extend into patches of dead tissue. The leaves will turn yellow and die. The lesions will also appear on the the stalk of the leaves, twigs and fruits. The fruits will then turn brown and black, and burst open. The released fluid is filled with bacteria that will contaminate the rest of the tree or other fruits in storage.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Agrimycin-100 (0.01%) or Streptocycline (0.01%), 3 times at 10 days interval. Subsequently spray Copper Oxychloride (0.3%) or Carbendazim (Bavistin 0.1%) once a month.&lt;br /&gt;
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==Die Back==&lt;br /&gt;
Die back (Botryodiplodia (Lasiodiplodia) theobromae aka Physalospora disrupta/quercuum/rhodina/glandicola) may occur any time of the year. It is characterized by advancing discoloration and darkening of the bark. It extends along the veins of the leaves, causing browning and upwards rolling of the margins. Eventually the leaves shrivel and fall. Twigs and branches dry and defoliate, and may drain a yellow-brown gummy fluid. It may look as if the tree has been exposed to a bush fire.&lt;br /&gt;
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[u]Control[/u]: Prune diseased twigs 10 cm below the affected area. Paste the cut ends with Copper Oxychloride (0.3%) and also spray Copper Oxychloride (0.3%) on affected trees.&lt;br /&gt;
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==Diplodia Stem-end Rot==&lt;br /&gt;
This fungus (Lasiodiplodia theobromae) may enter the mango where the skin or stem has been injured mechanically. It will form a black circle around the pedicel.&lt;br /&gt;
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[u]Control[/u]: Prevent mechanical injuries. After picking, submerge mangoes for 15 minutes in hot water (53°C) with Carbendazirn (0.1%).&lt;br /&gt;
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==Mango Black Blight==&lt;br /&gt;
Black blight is caused by a fungus (Capnodium mangiferum).&lt;br /&gt;
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==Mango Blight==&lt;br /&gt;
Mango blight is caused by Erwinia bacteria (Enterobacteriaceae). Many spots appear on the leaves which cause a reduction in growth and yield. &lt;br /&gt;
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[u]Control[/u]: Use Dithane M 45 at the rate of 1.7g/L (450 L water per acre).&lt;br /&gt;
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==Mango Malformation==&lt;br /&gt;
Mango malformation (Fusarium mangiferae) has been reported in Australia, Bangladesh, Brazil, Central America, Cuba, Egypt, India, Israel, Malaysia, Mexico, Pakistan, South Africa, Sudan, Switzerland, UAE and the USA. It is a disease caused by fusarium species [http://www.mycologia.org/content/94/4/722.full][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.6.456] (F. subglutinans from Egypt, Florida, Israel, Malaysia and South Africa, F. sterilihyphosum from Brazil and South Africa, and Fusarium sp. nov. and F. proliferatum from Malaysia)[http://www.ncbi.nlm.nih.gov/pubmed/18943188][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-96-0667 Free Full Text]. The disease is stimulated by relatively cool conditions; 10 to 27°C, and merely survive hotter conditions. In this disease the leaves and inflorescence of the mango tree are badly deformed and gradually dry up. There is no fruit setting and hence no production. &lt;br /&gt;
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[u]Control[/u]: Various fungicides, including Captan, Benomyl and Thiram have been reported to be effective to some extend. &lt;br /&gt;
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==Mango Scabs==&lt;br /&gt;
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Mango Scabs (Denticularia mangiferae aka Sphaceloma mangiferae aka Elsinoë mangiferae) survives on young tissues (young leaves, shoots, flower, fruits) of the mango tree only. Adult tissue is not susceptible. Its spores (and their germination) requires rain splash and free water to produce new infections.[http://www.cerambycoidea.com/titles/affa2004.pdf] Moisture conditions stimulate its growth. Though this parasite is not a fungus but in a persistent parasytic relationship with the mango tree, it produces symptoms that look somewhat familiar to those of anthracnose (a fungus) infections. It starts with small light- or dark brown or grey spots on the underside of leaves and fruit. These spots get bigger and darker with time. In the center of this lesion a cracked texture will appear. If the host tissue survives, lesions may develop into scar tissue. When infestation is severe, there is loss of leaves and fruit.&lt;br /&gt;
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[u]Control[/u]; Remove and destroy all fallen leaves, twigs, branches etc. Spray copper hydroxide or copper oxychloride on a very regular basis, particularly in the rainy season. Without chemical control, losses as high as 90% have been observed.[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Phoma Blight==&lt;br /&gt;
Phoma blight (Phoma glomerata) is a fungus that affects the fibre in the top layer of old leaves. It produces small, irregular, angular lesions. As the lesions grown, the colour changes from yellow-brown to cinnamon. Many small spots may form overwhelming patches and result defoliation of affected leaves. Phoma glomerate very effectively produces an enzyme that converts the remainders (glucose) of fibre (from the leaves) into it the main structural component (N-acetyl-D-glucosamine)[http://www.ncbi.nlm.nih.gov/pubmed/15553782] in its cell walls, thus enabling its own growth.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Benomyl (0.2%), and 20 days later spray 0.3% Miltox (Copper Oxychloride plus Zineb). Quadris (azoxystrobin) combined with thymol at a non-fungitoxic concentration produces much higher growth inhibition of Phoma glomerata than the fungicide alone.[http://www.ncbi.nlm.nih.gov/pubmed/22408641]&lt;br /&gt;
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==Powdery Mildew==&lt;br /&gt;
Powdery mildew (Oidium mangiferae) affects virtually all mango varieties. Characteristically, a white superficial powdery fungal grows on the inflorescence and tender leaves, stalk of panicles, flowers and young fruits. The affected flowers and fruits will pre-maturely drop, or fruit setting does not occur at all. The disease is initiated and developed by warm (&amp;gt;17°C) dry weather, but during the flowering stimulated by prolonged rains or mists and by cool nights. The disease has been reported in Brazil, India, Jamaica, Pakistan, Sri Lanka, South Africa and the U.S.A. &lt;br /&gt;
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[u]Control[/u]: Alternately spray wettable sulphur (0.2% ; 2 g Sulfex/L), Tridemorph (O.1% ; 1 ml Calixin/L) and Bavistin (0.1%) at 15 days interval. Start spraying when the panicle (flower cluster) starts to emerge. Or spray with Carbendazim (0.1%) or Tridemefon (0.05%) or wettable sulphur (0.3%) alone (3 days in a row).&lt;br /&gt;
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[u]Control-2[/u]: Similar to A. quisqualis AQ10 Biofungicide in commercial use for biocontrol of powdery mildew, Phoma glomerata (see [http://www.waiwiki.org/index.php?title=Mango#Phoma_Blight Phoma Blight] above) may act as mycoparasite of powdery mildew. Phoma glomerata can colonize and suppress development of powdery mildew.[http://www.ncbi.nlm.nih.gov/pubmed/10618259] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91841/ Full free text]&lt;br /&gt;
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==Red Rust==&lt;br /&gt;
Red rust (Cephaleuros virescens aka C. parasiticus) initially causes round and slightly elevated green-grey spots mainly on leaves. They turn into rusty red spots and may form bigger patches. This reduces the photosynthetic capacity of the leaves. In severely infected trees, twigs will remain stunted, the bark and twigs will get thick and the leaves will dry up and drop off. &lt;br /&gt;
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[u]Control[/u]: Spray Copper Oxychloride (0.3%) 3 times.&lt;br /&gt;
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==Sooty Mould==&lt;br /&gt;
Sooty mould (Meliola mangiferae) may be the result of too many insects in your orchard. Scale insects, mealy bug and hoppers secrete honey dew. The honey dew sticks to the leaves, feeding fungal growth. This results in a black sooty mould over the entire surface of leaves and twigs, affecting the photosynthetic capacity of the leaves. Uncontrolled, the tree may completely turn black.&lt;br /&gt;
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[u]Control[/u]: Prune and burn the affected branches. Spray Wettasulf (0.2%) with+ Metacid (0.1 %) and gum acacia (0.3%). Ants feed on the honeydew excreted by soft scales, preventing accumulation of sooty moulds, but they also protect the scales from natural enemies.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
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	<entry>
		<id>http://www.waiwiki.org/index.php?title=Mango&amp;diff=4244</id>
		<title>Mango</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Mango&amp;diff=4244"/>
		<updated>2014-12-12T16:02:53Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
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&lt;div&gt;Comparable to oranges and clementines, mangoes are among the most all-round fruits, regarding nutrients. Mangoes are cultivated in (sub)tropical regions all over the world.&lt;br /&gt;
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=Low-fibre, sweet mango species=&lt;br /&gt;
If you use a juice extractor like the [http://www.thekitchn.com/product-review-hurom-slow-juic-131492 Hurom] / [http://versapers.fr/ Versapers] (masticating slow juicer), the stringy fibres from species like Tommy Atkins or Haden will clog your juicer (more so than pineapples). The chamber will fill up with long fibres that simply never get discarded, so that you need to empty and clean it after every few mangoes. With mangoes like Kent (#11 on the list), you may endlessly (&amp;gt;100 mangoes) continue juicing without having to empty or clean the chamber in between, because the short fibres are immediately discarded as pulp (as with apples, pears, clementines).&lt;br /&gt;
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==Anwar Ratol==&lt;br /&gt;
[[Image:Anwar_Ratol.jpg|thumb|right| Anwar Ratol [http://freshfruitpakistan.blogspot.nl/2013/04/anwar-ratol-top-variety-of-mango.html © H. Khalid]]]&lt;br /&gt;
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* #1 (aka Anwer Rathol, Anwar Retaul or Anwar Rataul)&lt;br /&gt;
* Small size (av. 180 g), medium thick skin&lt;br /&gt;
* Matures from green to pale bright yellow &lt;br /&gt;
* Very sweet (TSS 26-28%)&lt;br /&gt;
* Fibreless flesh, medium juicy&lt;br /&gt;
* Originally from Rataul, in Bagpat district, in the province of Uttar Pradesh, India. &lt;br /&gt;
* Harvested from June to July (India and Punjab in Pakistan)&lt;br /&gt;
* Keeps well in storage&lt;br /&gt;
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==Alphonso==&lt;br /&gt;
[[Image:Alphonso.jpg|thumb|right|]]&lt;br /&gt;
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* #2 (aka Alphanso, Bombay)&lt;br /&gt;
* Small, roundish / oblong (av. 200 g.)&lt;br /&gt;
* Smooth, (from green to) pale greenish-yellow to deep orange peel (deepening with ripening)&lt;br /&gt;
* Smooth, firm flesh. Intense reddish-orange colour&lt;br /&gt;
* Sweet, somewhat sour (17% sugar; sugar/acid ratio: 39; TSS 21-23%) and flavoured&lt;br /&gt;
* Virtually without fibre (1%), medium juicy&lt;br /&gt;
* Originally from Western India&lt;br /&gt;
* Main production in Maharashtra and Gujarat&lt;br /&gt;
* Highest quality in Sindhudurg, Ratnagiri (Natwarlal plantation), Raigad and Thane districts&lt;br /&gt;
* Harvested from early April to early July (India) and July (some districts in Sindh, Pakistan)&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
* Tolerates high humidity&lt;br /&gt;
* Good keeping quality (up to 4 weeks at 11° C)&lt;br /&gt;
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==Dashehari==&lt;br /&gt;
[[Image:Dashehari.jpg|thumb|right|]]&lt;br /&gt;
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* #3 (aka Dasheri, Doshehri, Aman Dusehri or Dussehri)&lt;br /&gt;
* Small, oblong (av. 175 g)&lt;br /&gt;
* Matures from light green to light yellow, thin skin&lt;br /&gt;
* Sweet (TSS 21-22%), lemon yellow flesh&lt;br /&gt;
* Fibreless, medium juicy&lt;br /&gt;
* Very small stone&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Dasheri, Uttar Pradesh, India.&lt;br /&gt;
* Harvested from early June to July, mainly Malihabad, Uttar Pradesh (and Punjab)&lt;br /&gt;
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==Bagan Pali==&lt;br /&gt;
[[Image:Bagan_Pali.jpg|thumb|right|]]&lt;br /&gt;
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* #4 (aka Begun Palli, Began Phali, Banganapalli or Baganpali)&lt;br /&gt;
* Large (av. 550 g.), oval&lt;br /&gt;
* Sweet (TSS 18-20%)&lt;br /&gt;
* Fibreless, little juicy, yellow flesh &lt;br /&gt;
* Matures from dark green to yellow light green or yellow-orange&lt;br /&gt;
* Thin, smooth, shiny skin&lt;br /&gt;
* Originally from Sindh, Pakistan and/or Banganapalle in Andhra Pradesh, India.&lt;br /&gt;
* Harvested from July to August (Pakistan)&lt;br /&gt;
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==Sindhri==&lt;br /&gt;
[[Image:Sindhri.jpg|thumb|right|]]&lt;br /&gt;
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* #5 (aka Sindhry)&lt;br /&gt;
* Medium to large (av. 300 g., av. 400 g in Pakistan)&lt;br /&gt;
* Elongated with pointy curve&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 36; TSS 15-17%), flavoured&lt;br /&gt;
* Little fibre (3% - 5%), little juicy&lt;br /&gt;
* From green to deep matt lemon yellow thin peel (somewhat wrinkly) when ripe&lt;br /&gt;
* Deep yellow-orange flesh; soft and melting&lt;br /&gt;
* Originally from Mir Pur Khaas in Sindh&lt;br /&gt;
* Harvested from late May to July (Pakistan)&lt;br /&gt;
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==Chaunsa==&lt;br /&gt;
[[Image:Chaunsa.jpg|thumb|right|]]&lt;br /&gt;
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* #6 (aka Sammar Bahisht Chausa)&lt;br /&gt;
* Large (av. 350 g.), oblong&lt;br /&gt;
* Sweet (18% sugar; sugar/acid ratio: 58; TSS 18-20%)&lt;br /&gt;
* Soft, succulent flesh &lt;br /&gt;
* Medium fibre, large stone, medium juicy (delicious for eating, too stringy for the slow juicer)&lt;br /&gt;
* Thin, pale, matt yellow-greenish peel (wrinkly and slightly deeper when ripe)&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Pakistan&lt;br /&gt;
* Harvested mainly in the Rahim Yar Khan and Multan (Sahiwal) districts of Punjab in Pakistan&lt;br /&gt;
* Harvested from June to early September (Pakistan)&lt;br /&gt;
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==Maya==&lt;br /&gt;
&lt;br /&gt;
* #7&lt;br /&gt;
* Medium, round (av. 300 g.)&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 42; TSS 16-17%) and flavoured&lt;br /&gt;
* Red blush peel (with yellow tinge when ripe)&lt;br /&gt;
* Smooth, deep/pale yellow flesh (soft when ripe)&lt;br /&gt;
* Medium fibre, very juicy&lt;br /&gt;
* Harvested from mid-June to early July (Gambia)&lt;br /&gt;
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==Gopalbogh==&lt;br /&gt;
&lt;br /&gt;
* #8 (aka Gopal Bhogue)&lt;br /&gt;
* Small size (average 190 g.)&lt;br /&gt;
* Yellow-green peel, with small seeds&lt;br /&gt;
* Virtually without fibre&lt;br /&gt;
* Very sweet (21% sugar)&lt;br /&gt;
* Good quality mainly grown in Rajshahi region, especially in Chapainawabganj district (Bangladesh).&lt;br /&gt;
* Harvest from mid May to mid June (Bangladesh)&lt;br /&gt;
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==Sweet Elena==&lt;br /&gt;
[[Image:Sweet_Elena.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #9&lt;br /&gt;
* Large sized, ovoid (av. 350 g.)&lt;br /&gt;
* Thin, orange peel&lt;br /&gt;
* Yellow to orange flesh&lt;br /&gt;
* Little fibre&lt;br /&gt;
* Sweet (19% sugar)&lt;br /&gt;
* Originally (as a carabao variety) from Sta. Cruz, Zambales (Philippines)&lt;br /&gt;
* Harvest from March to April (Locloc, Palauig in northern Zambales, Philippines)&lt;br /&gt;
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==Ataulfo==&lt;br /&gt;
[[Image:Ataulfo.jpg|thumb|right|]]&lt;br /&gt;
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* #10 (aka Adaulfo, Adolfo, Champagne, Manila or honey mango, related to Alphonso)&lt;br /&gt;
* Small, oblong shape (av. 225 g.)&lt;br /&gt;
* With ripening, peel turns from pale green to gold-yellow (yellow to orange and wrinkles when fully ripe)&lt;br /&gt;
* Deep yellow flesh (gold when ripe), little fibre and thin pit&lt;br /&gt;
* Smooth, sweet (15% sugar), and buttery / creamy flesh&lt;br /&gt;
* Spicy flavour &lt;br /&gt;
* Best temperature is 28°C, tolerates 1100 to 3000 mm annual rainfall &lt;br /&gt;
* Originally from Chiapas, Mexico&lt;br /&gt;
* Harvest from mid-October to late December (Ecuador); February to August (Ghana)&lt;br /&gt;
* May be stored in the fridge for 2 weeks&lt;br /&gt;
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==Kent==&lt;br /&gt;
[[Image:Kent.jpg|thumb|right|]]&lt;br /&gt;
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* #11&lt;br /&gt;
* Large, rounded oval (500 to 800 g.)&lt;br /&gt;
* Smooth, waxy thick peel (green with some yellow and red; yellow increases with ripening)&lt;br /&gt;
* Soft, melting, juicy flesh (from deep yellow to deep orange when fully ripe)&lt;br /&gt;
* Limited fibre (dry weight: 0.4 - 2%), small seed&lt;br /&gt;
* Sweet (13% sugar; 14ºBrix [http://www.m.elewa.org/JAPS/2013/17.3/5.pdf])&lt;br /&gt;
* Originally from Mexico / Florida&lt;br /&gt;
* Harvest from January to March and May to August (Ghana)&lt;br /&gt;
* Large tree (up to 20 m.)&lt;br /&gt;
* No storage keeping below 13°C&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
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=Mango Cultivation=&lt;br /&gt;
==Conditions==&lt;br /&gt;
The best soil for mango trees has a pH between 5.5 and 7.5 (high pH may cause leaf tip burn). The temperature range for growing mangoes is 15 to 40°C. Mango does best within a temperature range of 24 to 27°C. The Indian race is in general intolerant of humidity (susceptible to anthracnose), the Philippine race tolerates excess moisture. The life span of each tree is approximately 100 years. Economic bearing life is 30 to 50 years.&lt;br /&gt;
Mango trees need deep soil (up to 2 m) for their root systems. Grafted saplings yield a more superior quality of fruit. Fruits are borne by mango trees from 4 to 5 years after planting. Full bearing starts at 6 to 7 years.&lt;br /&gt;
Grafted mango plants start bearing blossoms one to two years after planting, provided that there is no high humidity or intense rain during the flowering period (dryness induces flowering).&lt;br /&gt;
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==Rootstock Propagation==&lt;br /&gt;
[[Image:Mango_grafting.jpg|thumb|left| [http://www.muftisays.com/blog/ummi+taalib/2718_20-03-2012/shaikhmurid-relationship.html © ummi taalib]]] &lt;br /&gt;
[[Image:Grafted_seedling.jpg|thumb|centre| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
[[Image:Grafted_seedlings.jpg|thumb|right| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
Trees grown from mango seeds (and naturally, sexually propagated) will be very different from the tree those mangoes came from (such &amp;#039;wild mangoes&amp;#039; usually taste bad). That is why rootstocks (raised from seeds) are asexually propagated. Matured seedlings (about 60 cm tall) are grafted by inarching; a bud from a tree producing good quality mangoes is artificially attached to the rootstock. This will create a new tree with two parts: the lower (wild) part and the upper part, which is an exact copy of the tree you took this bud from. Any bud growing from the lower part needs to be pruned off immediately. The upper part needs to grow and produce branches, and eventually fruits. During the first month after planting, the young trees need to be irrigated every 3 days. During the next 2 months, they need to be irrigated once a week. After that they should not be watered for a couple of months. Each year they need a dry season of a few months. Young plants need 10 to 30 kg organic manure per plant. Don&amp;#039;t use a chemical fertilizer on young plants. Once the tree is 2 years old, you may start using a controlled release fertilizer (with higher nitrogen), like 12-5-9 or 12-8-34 (= ratios N:P:K); 1 tbsp. in 1 L warm water / meter tree height. Or just manure.  &lt;br /&gt;
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Rootstock branches should always be pruned off. To prevent viral infections, only prune using a very sharp, sterilized knife (sterilize on the spot with a propane torch).&lt;br /&gt;
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==Production technology== &lt;br /&gt;
Space mango trees about 12 meters apart. Adult mango trees need about 500-750 mm of water per year. In the wet season irrigated once per 8 to 10 days. In the dry season once per 15 to 20 days. Mango trees need dry and wet spells alternately (also kills various pathogens). Don&amp;#039;t fertilize mature trees during fruit bearing, and not too much irrigation. If during the blooming season the soil contains much nutrients (fertilizer) and water, the tree will grow, but not flower and bear fruits. &lt;br /&gt;
You may either:&lt;br /&gt;
* A: Apply 80 to 100 kg manure per year (eg humanure from [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets]), after all fruits are picked. Or:&lt;br /&gt;
* B: Apply 3-4 kg SSP, 2-3 kg Potassium Sulphate and 2-3 kg Urea before flowering. Plus 2-3 kg Urea in 2 doses after fruit setting. Or:&lt;br /&gt;
* C: Use a controlled release fertilizer once a year, such as 4-4-8 (= ratios N:P:K), after all fruits are picked (1 tbsp. in 1 L warm water / meter tree height).&lt;br /&gt;
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After fruit harvest, prune off diseased, dried, broken branches and those touching the ground. Every 3 to 4 years about 15 to 20% of old wood should be removed.&lt;br /&gt;
Picking should be done when the fruit is fully developed and mature. Natural drop of the fruit is the main indication that the fruit is ready for picking. Expected yields vary from 40 to 100 kg per tree.&lt;br /&gt;
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One cannot rear any animals (except for carnivores) on land with mango trees, as mango leaves are deadly poisonous.&lt;br /&gt;
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=Bugs=&lt;br /&gt;
&lt;br /&gt;
==Aphids==&lt;br /&gt;
[[Image:Propylea_quatuordecimpunctata.jpg|thumb|right| Predatory ladybird [http://www.colpolon.biol.uni.wroc.pl/propylea%20quatuordecimpunctata.htm]]]&lt;br /&gt;
[[Image:Aphids_on_mango.png|thumb|left| Aphids [http://www.infonet-biovision.org/default/ct/124/crops#_1804_1202 © Varela]]]&lt;br /&gt;
[[Image:Mallada_sp_2.jpg|thumb|right| Mallada sp. © Texas University]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
&lt;br /&gt;
Aphids (plant lice; Toxoptera odinae) are small (1-4 mm), living in clusters. They pierce plant tissue and suck the sap of the leaves, which may cause the leaves to bend, twist or roll up. Aphids also transmit plant viruses. Some species produce winged offspring, &amp;quot;alates&amp;quot;, that may readily disperse. Dry (and warm) weather stimulates increases in aphid numbers. Some species of ants (eg Dolichoderus cuspidatus and Formica aerate) protect and &amp;#039;farm&amp;#039; aphids, feeding on the honeydew released by the aphids&amp;#039; alimentary (gut) canals. Insects secreting honeydew may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould] &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Plant flowering plants at the boarders to attract beneficial insects. Plant &amp;#039;trap crops&amp;#039; (dill, nasturtiums, timothy grass) to monitor aphid numbers. Check the trap crops every 3 days. Infested trap crops need to be burned. Aphid&amp;#039;s natural enemies include predatory ladybirds (eg Propylea quatuordecimpunctata), hooverfly larvae, parasitic wasps, aphid midge larvae, crab spiders (Thomisidae) and lacewings (Neuroptera). Exposing aphid populations to natural predators may be successful particularly when its hot, because once bitten they release an alarm pheromone and the others all jump off the plant. Jumping aphids may experience a heat shock (&amp;gt; 35°C) since the ground may be much warmer than the shady tree. This heat shock usually sterilizes the aphids (killing the bacteria on aphids that provide nutrients essential for aphid reproduction). [http://www.sciencedaily.com/releases/2007/04/070419172046.htm]&lt;br /&gt;
&lt;br /&gt;
[u]Crab spiders (Thomisidae)[/u]; Crab spiders are ambush hunters (they don&amp;#039;t build webs) that not just eat aphids, but may also hunt ants that farm aphids. Some of these crab spiders, Xysticus sp, may however not just eat ants, but also predatory beetles (that eat aphids).&lt;br /&gt;
&lt;br /&gt;
[u]Green lacewings (Chrysopidae)[/u]; One may buy millions of lacewings (as captive-bred eggs) for biological pest control, as reared for that purpose in many countries. A female lacewing may plant over 100 eggs on plants infested with aphids. These eggs hang on a thin 1 cm long stalk, most often under a leaf. The larvae of most species of lacewings are specialised predators that eat aphids (and coccids such as mango scales, and caterpillars). These larvae sway their heads from left to right, and back, until they strike something they can eat. They have mouthparts that can pierce and suck the aphids. Many (adult) lacewing species also feed on nectar and pollen, but particularly Chrysopidae (eg Mallada signata, Mallada boninensis) are mainly predatory (one adult may eat 15 aphids per day). One may attract Chrysopidae by planting crops that attract them (mainly Asteraceae and Apiaceae), such as calliopsis (Coreopsis), cosmos (Cosmos), sunflowers (Helianthus), dandelion (Taraxacum), dill (Anethum) and angelica (Angelica). &lt;br /&gt;
&lt;br /&gt;
[u]Chemicals[/u]; If you have no other option and immediate action is required: Use Folido 50% EC at the rate of 0.45 L per 450 L water / acre. (also kills natural aphid enemies)&lt;br /&gt;
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==Fruit Flies==&lt;br /&gt;
[[Image:Fruit_fly_maggots.jpg|thumb|centre| Maggots [http://www.infonet-biovision.org/default/ct/124/crops © A. M. Varela]]]&lt;br /&gt;
[[Image:Bactrocera_invadens.jpg|thumb|left| B. invadens [https://www.flickr.com/photos/iaea_imagebank/6776806998/ © IAEA]]]&lt;br /&gt;
[[Image:Fopius_arisanus.jpg|thumb|right| F. arisanus [http://www.tephritid.com/digital.php?act=page&amp;amp;pid=28&amp;amp;id=25 © tephritid.com]]]&lt;br /&gt;
[[Image:Ceratitis_capitata.jpg|thumb|left| C. capitata [http://josedotinzulza.edublogs.org/2013/04/21/how-much-damage-can-make-a-fly/ © José Inzulza]]]&lt;br /&gt;
[[Image:Diachasmimorpha_longicaudata.jpg|thumb|right| D. longicaudata [http://www.oocities.org/brisbane_parawasps/FruitFlyParasitoid.htm]]]&lt;br /&gt;
[[Image:Ceratitis_fasciventris.jpg|thumb|left| C. fasciventris [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Oecophylla_longinoda.jpg|thumb|right| O. longinoda [http://www.alexanderwild.com/Ants/Taxonomic-List-of-Ant-Genera/Oecophylla/i-tMfJXKG © A.Wild]]]&lt;br /&gt;
[[Image:Ceratitis_cosyra.jpg|thumb|left| C. cosyra [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Jackson_trap.jpg|thumb|right| Jackson trap]]&lt;br /&gt;
[[Image:Ceratitis_rosa.jpg|thumb|left| C. rosa [http://wzciq.wenzhou.gov.cn/art/2011/1/12/art_6811_63595.html © wzciq]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis eating fly [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
In 2013, the export of mangoes from Ghana and 27 other African countries was banned from major international markets mainly due to fruit fly infestation. The European Union imposed a ban from May 1 2014, on import of mangoes from India, also due to fruit fly infestation. &lt;br /&gt;
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The fruit flies (aka peacock flies; 4-7 mm long) that may form a very severe mango cultivation pest are not the Drosophilae (2-4 mm long), but the colourful Tephritidae family (&amp;gt; 5000 species). These attack mango fruits throughout the season and lay their eggs right under the (affected) skin of mature green or ripe fruits. They have three generations and multiply very rapidly. Within one or two days the eggs hatch into white maggots. The maggots feed on the fruit, which starts to rot. After 4 to 17 days, the maggots make holes in the skin and leave the fruit to pupate. &lt;br /&gt;
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The main fruit fly pests of mango in Africa are Ceratitis capitata (Medfly), C. fasciventris, C. rosa and C. cosyra (the most destructive). C. cosyra is however rapidly displaced by Bactrocera invadens (endemic to Sri Lanka)[http://www.ncbi.nlm.nih.gov/pubmed/19610411][http://www.ncbi.nlm.nih.gov/pubmed/22251685], being predominantly a low-land pest.[http://www.ncbi.nlm.nih.gov/pubmed/16923206] B. invadens populations quickly increase with the onset of the raining season[http://www.ncbi.nlm.nih.gov/pubmed/19449630] and also lay aggs in young fruits. Fruit fly populations peak in the late dry season.[http://www.ncbi.nlm.nih.gov/pubmed/17598527]&lt;br /&gt;
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[u]Control[/u]: Collect all the fallen and affected fruits and bury them deep (&amp;gt;50 cm) into the soil. Or you can use them to breed maggots in a sealed room for fish feed. Natural enemies of tephritids include Diapriidae (tiny wasps; &amp;lt;8mm), Braconidae (also parasitoid wasps) and Oecophylla longinoda (Latreille; a weaver ant). &lt;br /&gt;
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C. cosyra, but particularly C. fasciventris and Medfly may be effectively controlled by using the host-marking pheromone.[http://www.ncbi.nlm.nih.gov/pubmed/23356072] B. invadens [http://www.ncbi.nlm.nih.gov/pubmed/24293246], the Mediterranean fruit fly (C. capitata) and the Mexican fruit fly (Anastrepha ludens) may be effectively controlled by the &amp;#039;sterile insect technique&amp;#039; (releasing overwhelming numbers of sterile insects). The effects of sterile flies are greater than those for parasitoid wasps, but they are complementary.[http://www.ncbi.nlm.nih.gov/pubmed/15568340] &lt;br /&gt;
Medfly eggs and instars (right under the peel) may be killed by immersing Ataulfo mangoes for 95 min in warm water at 47°C, also positively modifying pH (producing more palatable fruits), but can also produce a loss of firmness and weight (5%).[http://www.ncbi.nlm.nih.gov/pubmed/23356057] B. invadens is no more heat tolerant than Medfly.[http://www.ncbi.nlm.nih.gov/pubmed/21404834] &lt;br /&gt;
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[u]Bait traps[/u]; &lt;br /&gt;
Pheromone traps (85% methyl-eugenol, 5% Naled (insecticide), 10% saturated sugar) can be used for trapping male flies (Jackson trap). A three-component attractant (ammonium acetate, putrescine, and trimethylamine lures) may be used to trap female population of Medfly. (No wind: 28 m. range).[http://www.ncbi.nlm.nih.gov/pubmed/21061993] Malathion-bait sprays and Phloxine B (a xanthene dye; D&amp;amp;C Red #28) are equally effective.[http://www.ncbi.nlm.nih.gov/pubmed/11777044]&lt;br /&gt;
Of six commercial food-based attractants, Nulure captured the greatest proportion of females: 74% compared with 51-68%. But Mazoferm E802 (with or without Spinosad) and Torula yeast captured 2.4-2.6 times more females and 3.4-4.0 times more males than Nulure (also more effective than GF-120, Hymlure and Biolure).[http://www.ncbi.nlm.nih.gov/pubmed/24665714]&lt;br /&gt;
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[u]Fopius arisanus (Sonan)[/u]; F. arisanus is a tiny but most effective[http://www.ncbi.nlm.nih.gov/pubmed/12241567] and the most dominant parasitoid wasp. As parasitism increases, fruit fly infestation decreases.[http://www.ncbi.nlm.nih.gov/pubmed/17598524] Of 3 commonly used bait sprays, which all suppress fruit fly populations, Spinosad and Phloxine B bait sprays are less harmful to these wasps than Malathion bait spray. [http://www.ncbi.nlm.nih.gov/pubmed/11561838]   &lt;br /&gt;
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[u]Diachasmimorpha longicaudata[/u]; D. longicaudata (longtailed fruit fly wasp) is the most important parasitoid wasp used as part of integrated pest management programs against fruit flies such as B. invadens and Ceratitis species. They lay one or more (when hosts are scarse) eggs in fruit fly larva.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014816/] Introduction of D. longicaudata poses minimal competitive risk to F. arisanus.[http://www.ncbi.nlm.nih.gov/pubmed/12241567]&lt;br /&gt;
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[u]Oecophylla longinoda[/u]; O. longinoda is a predator weaver ant that may eat whatever insect or bug they may catch, including caterpillars, beatles, honey bees, driver ants (Dorylus nigricans Ill.) and larvae. O. longinoda is a natural enemy of fruit flies as it eats their larvae. This weaver ant is fiercely territorial and will keep other species of ants (like aphids-farming black ants) at bay. The ants continuously patrol the tree that they live in and may also catch egg-laying female fruit flies in the act, and eat them. B. invadens and C. spp numbers on Kent mangoes may be greatly reduced by Oecophylla longinoda.[http://www.ncbi.nlm.nih.gov/pubmed/17598527] &lt;br /&gt;
Weaver ants, however, also feed on honeydew. They may farm a wide range of honeydew-producing Homoptera (bugs with sucking mouthparts, including scales, aphids and mealybugs), but are mostly associated with Coccids (Saissetia; no virus vector). Only when other Coccids are scarce or unavailable, O. longinoda may farm aphids and mealybugs (unarmoured scale insects that are virus vectors). Mainly in small trees and shrubs, the mechanical damage caused by Coccids is related to the size of the attendant ant colony. Dense populations of O. longinoda are supported by interplanting with clove.[http://www.cabdirect.org/abstracts/19540500227.html;jsessionid=36A454B839F0DEEADFA83F99A7206E6D]&lt;br /&gt;
For the protection of their harvested honeydew, these weaver ants weave a translucent silken tent around living leaves. They use the silk produced by their larvae. It is claimed that pheromones produced by the weaver ants repel egglaying fruit flies. A study in Senegal, however, found that pheromones produced by O. longinoda did hardly repel egglaying females of B. invadens.[http://www.ddrn.dk/filer/forum/File/Abstract_Christina_Abel.pdf]&lt;br /&gt;
On the other hand, trees colonized by O. longinoda (in Ghana) had only 6 to 10% fly infestation, compared to 3% fly infestation in trees treated with Cypermethrin plus Dimethoate. (1614 mg per tree)[http://ugspace.ug.edu.gh/handle/123456789/2387] &lt;br /&gt;
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[u]Praying mantids[/u] may also catch and eat fruit flies. Praying mantids are opportunist predators and do not farm any insects or other bugs. Particularly young mantids may not yet be too large to prey on fruit flies (tephredids). The younger and/or smaller the praying mantids are, the smaller the insects (and any other living creature they may catch) they prey on. Praying mantid nymphs initially feed on aphids and fruit flies (drosophila), and as they grow, they skip to larger preys, such as tephritids, and eventually moths and even much bigger preys.&lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option, GF-120 (Dow chemical) may greatly (81-89% after 7-10 weeks) reduce the number of B. invadens pupae per kg mango.[http://www.ncbi.nlm.nih.gov/pubmed/19449630] Or use Dioptries 80% at the rate of 1 L in 450 L water or Malathion 57% at the rate of 0.5 L to 450 L water / acre. Broad-spectrum insecticides are most damaging to fruit flies&amp;#039; natural enemies such as parasitoid wasps.[http://www.ncbi.nlm.nih.gov/pubmed/19886446] &lt;br /&gt;
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==Long-horn Beetles==&lt;br /&gt;
[[Image:Stem_borer.jpg|thumb|left| Stem Borer [http://www.bitterrootrestoration.com/mango/stem-borer-batocera-rufomaculata.html ©]]]&lt;br /&gt;
[[Image:Dastarcus_helophoroides.jpg|thumb|right| D. helophoroides[https://www.ffpri.affrc.go.jp/labs/seibut/bcg/bcg00284.html ©]]]&lt;br /&gt;
[[Image:Batocera_rufomaculata_2.jpg|thumb|left| Batocera rufomaculata [http://agritech.tnau.ac.in/crop_protection/crop_prot_crop_insectpest%20_Mango_pest&amp;amp;disease.html © TNAU]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Batocera_rubus.jpg|thumb|left| Batocera rubus [http://163.20.112.34/~afu/77v.htm © ]]]&lt;br /&gt;
[[Image:Batocera_baby.jpg|thumb|left| baby Batocera [https://www.flickr.com/photos/bondingtool/14291758294 © Samantha - thebondingtool.com]]]&lt;br /&gt;
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* [u]The (red-spotted) Stem Borer (Batocera rufomaculata)[/u], is a large long-horned beetle (up to 5 cm long body). It cuts incisions on the bark and lays its eggs (up to 200) inside the cuts or cracks of the (damaged) tree bark, trunk or cavities (eg exposed roots). The beetles feed on the bark of living trees and eat the green of young shoots. The grub of the stem borer pupates and feeds inside the stem of mango trees (and other fruit trees, including durian, figs, avocado, jackfruit and cashew nuts), creating a tunnel towards the surface (up to 2 cm in diameter), eventually becoming a beetle. Sap (from the tree) and frass (or coarse sawdust, from the larvae) may drip from the holes. Older larvae may also tunnel deeper in the trunk or branches. This causes drying of branches, and the tree may die. The incidence of infestation of trunk borer is highly influenced by maximum temperature. Infestation is highest when temperature and humidity are high.[http://www.ncbi.nlm.nih.gov/pubmed/24498801] For reproduction, it needs to lay its eggs in a stem that is at least 7 cm in diameter. The Stem Borer has been observed in the west coast of Africa, Madagascar, Mauritius, Réunion, Seychelles, Turkey, Israel, Syria, Iraq, Iran, Pakistan, India, Bangladesh, Sri Lanka, China, Nepal, Thailand, Indonesia, Malaysia and the West Indies. Adults may live up to 4 months. Full grown larva may be 8 to 10 cm long, and may live up to a year, causing a lot of damage. Larval and prepupal + pupal stage lasts about 280 and 24-29 days.[http://www.actahort.org/books/787/787_41.htm]&lt;br /&gt;
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* [u]The (yellow-spotted) Mango Longhorned Beetle (Batocera rubus)[/u] is also a wood borer and about equally large as the Stem Borer. Full grown larvae are 6 to 8 cm long. It attacks various fruit trees, including mango, breadfruit and figs, but also bonsay trees, rubber trees and freshly felled timber. Batocera rubus has been observed in Pakistan, India, Bangladesh, China, Cambodia, Indonesia, Malaysia, Phillipines, Thailand, Taiwan and Vietnam.&lt;br /&gt;
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[u]Natural enemies[/u]; Tiny parasitoid wasps such as Oobius agrili sp.n. (1 mm long), Spathius agrili, Avetianella batocera, Tetrastichus planipennisi and Avetianella xystrocerae sp.n (1.5 mm long) have been shown effective against (the larvae of) some specific wood-boring beetles (Agrilus planipennis, Agrilus sexsignatus and Xystrocera globosa)[http://www.emeraldashborer.info/files/Zhang%20et%20al%202005%20Oobius_Leah.pdf][http://www.fs.fed.us/nrs/pubs/other/2013/2013_McManus_FHTET-13-01.pdf]. Batocera rufus (the mango longhorn beetle), is parasitized (its eggs) by  Louricia ovivora, gen. et sp. n.(a moth), and Ooencyrtus batocerae, sp. n.(a wasp) [http://cabdirect.org/abstracts/19360501471.html;jsessionid=A9E2E64A372836E8E24F2B4E97287DC9] Oobius batocera is an opportunistic parasytic wasp that parasitizes various insect eggs, including beetle eggs. [http://www.nhm.ac.uk/research-curation/research/projects/chalcidoids/database/synonyms.dsml?FamilyCode=EE&amp;amp;ValFamTrib=&amp;amp;VALGENUS=Oobius&amp;amp;VALSPECIES=batocerae&amp;amp;VALAUTHOR=(Ferri%E8re)&amp;amp;VALDATE=1936&amp;amp;ValidAuthBracket=false&amp;amp;HOMCODE=0&amp;amp;&amp;amp;listPageURL=listChalcids.dsml%3FSuperfamily%3DChalcidoidea%26Family%3DEncyrtidae%26Genus%3DOobius] &lt;br /&gt;
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There are also various mites that parasitize Batocera longhorned beetles.[http://www.jstor.org/discover/10.2307/3225250?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21103843600301] Tetrapolipus diastocerae n. sp., Tetrapolipus afrobatocerae n. sp., Tetrapolipus ramarajui n. sp. and Tetrapolipus seemani n. sp. are described. Tetrapolipus afrobatocerae has been observed in Africa, Tetrapolipus hunteri in Australia.[http://www.researchgate.net/publication/233099056_The_Genus_Tetrapolipus_(Acari_Podapolipidae)_Parasites_of_Tropical_and_Semitropical_Cerambycidae_(Coleoptera)_New_Distribution_Records_and_Descriptions_of_Four_New_Species] Tetrapolipus sulawesiensis is a mite that parasitizes Batocera herculus.[http://d.wanfangdata.com.cn/periodical_dwfl200202010.aspx] &lt;br /&gt;
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[u]Dastarcus helophoroides[/u]; This predator/parasitoid beetle (originally from China and Japan) is an important natural enemy of longhorned beetles. In the larval stage they attack various long horn beetles, including Batocera horsfieldi and Anoplophora glabripennis. Populations are attracted specifically by the frass of their original host. Eggs are laid twice a year, near the host entrance hole, frass-extrusion holes or larval tunnel walls, guided by semiochemicals. The freshly hatched (at about 21°C) first instars (0.7 mm in length) have legs, and actively move to the host. They bite and paralyze the host, and then suck hemolymph and degenerated tissues from the dead or paralyzed prey. Adults can live over 4 years. [http://link.springer.com/article/10.1007%2Fs10526-009-9224-y#page-1] Mass production is feasible if hatched larvae are fed cerambycid larvae until they are about 8 mm in length, and subsequently reared on artificial diet (silkworm pupa powder, dry yeasts, yeast extract, sucrose, peptone, squid liver oil, preservatives and distilled water). The full grown larvae are about 18 mm in length.[http://link.springer.com/article/10.1023%2FA%3A1009936609401#page-1] Adults don&amp;#039;t readily disperse; they need to be released on each tree separately. And even if you release them at 1.2 m height, their effectiveness decreases with increasing height in the tree. Yet their effectiveness may be very high; up to 85% host mortality. [https://www.ffpri.affrc.go.jp/labs/kanko/401-1.pdf]&lt;br /&gt;
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[u]Control[/u]; The stem borer is active during the night and attracted by light, so that light traps may be effective. Bacillus thuringiensis is a bacterium commonly used as a biological pesticide, and naturally present in the gut of various caterpillars. This bacterium is toxic to many insects. A specific strain of this bacterium, Bacillus thuringiensis ZQ-89 is also toxic to adult long-horned beetles.[http://www.ncbi.nlm.nih.gov/pubmed/21332894] Prevent the stem borer from laying eggs in the bark by applying a thick layer of coaltar on the stem. Or one may paint (at 0.5%) adult mango trees with monocrotophos or phosalone.[http://www.cabdirect.org/abstracts/19790379463.html] One may also specifically apply the insecticides to the potentially affected sites (cracks and cavities), and to twigs and young shoots to deter feeding by adults. One may poke with a hard wire to physically damage, and/or inject insecticides where larvae are suspected. Chloroform, ethyl acetate, Metasystox [demeton-S-methyl] and a mixture of petroleum and kerosene oil (at 5 ml/bore) and ethylene dibromide (at 3 ml/bore) gave 100% mortality of B. rufomaculata larvae.[http://www.cabdirect.org/abstracts/19881107545.html] Plaster the holes with wet clay, aluminium phosphide tablets (3 g/hole) and dichlorvos (0.1% spray).[http://www.cabdirect.org/abstracts/19790379463.html] Among the chemical treatments, Imidacloprid 17.8% SL, Thiamethoxame 25% WG was found best in management of mango stem borer.[http://www.gifre.org/admin/papers/gjbahs/management-vol-2-4-gjbahs.pdf]&lt;br /&gt;
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==Mango Hoppers==&lt;br /&gt;
[[Image:Idioscopus_niveosparsus.jpg|thumb|left| I. niveosparsus [http://163.20.112.34/~afu/641x.htm ©]]]&lt;br /&gt;
[[Image:Mallada_basalis.jpg|thumb|right| Mallada sp. [http://3insect.blogspot.nl/2012/06/mallada-basalis.html © Chen KunTsan]]]&lt;br /&gt;
[[Image:Idioscopus_clypealis.jpg|thumb|left| I. clypealis [http://163.20.112.34/~afu/641y.htm ©]]]&lt;br /&gt;
[[Image:Mallada_signata_3.jpg|thumb|right| Mallada signata [http://www.oocities.org/brisbane_lacewings/Chrysopidae.htm © Keith Power]]]&lt;br /&gt;
[[Image:Idioscopus_nitidilus.jpg|thumb|left| I. nitidilus [http://tech.groups.yahoo.com/group/pestnet/message/6414 ©]]]&lt;br /&gt;
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Mango hoppers:&lt;br /&gt;
* Idioscoynio chypeabis&lt;br /&gt;
* Idioscopus niveosparsus&lt;br /&gt;
* Idioscopus clypealis&lt;br /&gt;
* Idioscopus nitidulus&lt;br /&gt;
* Amritodus atkinsoni aka mango leafhopper&lt;br /&gt;
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Mango hopper nymphs as well as adults puncture and suck the sap of tender parts of the mango plant. Heavy drainage will cause the leaves to curl and dry. Infested flowers will shrivel and turn brown. Hoppers also secrete honeydew, which may cause sooty mould, inhibiting the leaves from obtaining energy from daylight. Sufficient spacing of trees and pruning of overlapping branches are essential in preventing hopper infestation, as shade (and high humidity) favour hopper multiplication.&lt;br /&gt;
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[u]Natural enemies[/u]:&lt;br /&gt;
Parasites: Aprostocetus sp., Gonatocerus sp. and Polynema sp. parasitize eggs of Idioscopus clypealis and Idioscopus nitidulus.[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
A preparation of the fungus Beauveria bassiana is also somewhat effective against mango hoppers. Chrysopa lacciperda [Plesiochrysa lacciperda] and Mallada boninensis predate on on nymphs of I. clypealis, I. nitidulus and Amritodus atkinsoni.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9]&lt;br /&gt;
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[u]Mallada boninensis[/u]; This green lacewing is an effective [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=5&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFQQFjAE&amp;amp;url=http%3A%2F%2Fwww.researchjournal.co.in%2Fonline%2FIJAS%2FIJAS%25203(2)%2F3_A-164-166.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNGZ9IidKvjO6s1zNs0HvbUMpKKPBg] generalist predator, feeding on mealy bugs (Mani and Krishnamoorthi, 1987), white flies (Selvakumaran et aI., 1996), bollworms and aphids (Kabissa et al., 1996) and on nymphs of Aphis gossypii, Aphis craccivora and Rhopalosiphum maidi [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=3&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEAQFjAC&amp;amp;url=http%3A%2F%2Fwww.ets-staffing.com%2Fcurrentbiotica%2Fjournals6-issueiii%2Fcb-6-3-short-notes-3.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNETNWfEOrZznoJjj0ZrFFOINJ2liw], blackflies, psylla and leaf miner. Green lacewings are recommended for the integrated pest management programme (Nehare et al., 2004). M. boninensis feeds well on Corcyra cephalonica eggs[http://cabdirect.org/abstracts/20113350526.html;jsessionid=69EA3BE15508EF9C029CE688C342FD4D] (laboratory host) C. cephalonica can be mass reared on Jowar along with groundnut, streptomycin, vitamin complex, Na and K salts.&lt;br /&gt;
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[u]Control[/u]: &lt;br /&gt;
Spray Imidacloprid (0.005%; 0.3 ml/L water), acephate 75SP (1.5 g/L water), Etofenprox (0.03%), Phosalone (1.5 ml/L water), Carbaryl (0.15%; 3 g/L water), Monocrotophos (0.04%), Phosphamidon (0.05%) or Methyl Parathion (0.05%). &lt;br /&gt;
* Spray 3 times; First spray should be given during the early stage of flower formation. The second spray should be given at a flower size of 6 to 8 cm, still before blooming. The third spray should be given after fruit setting, when the fruits are the size of a pea. Or:&lt;br /&gt;
* First spray should be given during the early stage of flower formation. The second spray should be given 2 weeks later. Or:&lt;br /&gt;
* Spray 3 times; First spray of imidacloprid (0.005%; 0.3 ml/L water) should be given during the early stage of flower formation. The second spray, thiamethoxam (0.005%; 0.2 g./L water) or acephate 75SP (1.5 g/L water) should be given at after fruit setting. If hopper infestation persists, the third spray, of carbaryl (0.15%; 3 g/L water) should be given prior to fruit maturation.&lt;br /&gt;
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Imidacloprid residues persist in peel for 60 days and in pulp for 50 days. Mature Dashehari fruits at harvest (after 85 days of spraying) were free from imidacloprid residues.[http://www.ncbi.nlm.nih.gov/pubmed/23196371]&lt;br /&gt;
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==Mango Midges==&lt;br /&gt;
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[u]Inflorescence Midge[/u]&lt;br /&gt;
[[Image:Platygaster_sp.jpg|thumb|right| Platygaster sp. [http://liuzhen.000space.com/insect/organism.php?id=1367&amp;amp;type=list]]]&lt;br /&gt;
[[Image:Inflorescence_midge.jpg|thumb|left| [http://www.bitterrootrestoration.com/mango/inflorescence-midge-erosomyia-indica.html Erosomyia indica ©]]]&lt;br /&gt;
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This midge (Erosomyia indica) lays eggs in new inflorescence (preventing flower and fruit set) and new leaves around it, and then the larvae will eat their way through. The maggots penetrate and feed on the tender parts of the plant, such as shoots, buds and flower buds. The flowers will dry and fall off. For pupation, the mature larvae will drop into the soil. The adult midge lives only one day and doesn&amp;#039;t cause any damage. Maggots from eggs laid on new fruits will eat their way into the fruit, which will turn yellow and drop.[http://www.actahort.org/books/231/231_15.htm] The inflorescence midge is an important pest in India in particular.&lt;br /&gt;
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Infloresence midge is parasitized by Platygaster sp., Eupelmus sp. and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19881115612.html;jsessionid=E4CDBFFBEE75C82EDA1BA2CA7B5F1DD2], which all also parasitize Gall Midges. The inflorescence midge is also parasitized by Aprostocetus sp.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9] and Mirufens longifunculata. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false]&lt;br /&gt;
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Another mango midge, Procystiphora mangiferae is parasitized by Pirene sp. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], which also parasitizes mango gall midges.&lt;br /&gt;
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Chemicals: If you have no other option, Parathion, Dimethoate, Phosphamidon and Endosulfan much reduced infestations on mango shoots.[http://www.cabdirect.org/abstracts/19740517480.html;jsessionid=59B14F930CB8EDFD2F1B50F1B04C7692] The most effective insecticide for control appeared to be phosphamidon (Dimecron), whether mixed with diazinon or not, as a foliar spray. [http://www.cabi.org/isc/abstract/19770549886]&lt;br /&gt;
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[u]Gall midges[/u]&lt;br /&gt;
[[Image:Mango_galls.jpg|thumb|left| [http://www.infonet-biovision.org/default/ct/124/crops © A.M. Varela]]]&lt;br /&gt;
[[Image:Eupelmus_urozonus.jpg|thumb|right| Eupelmus urozonus [http://www.biolib.cz/en/image/id150459/ © Miroslav Fiala]]]&lt;br /&gt;
[[Image:Mango_galls_P_matteiana.jpg|thumb|left| P. matteiana galls [http://www.pests.blogfa.com/8907.aspx © RJ Gagné]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Gall_midget_exit_holes.jpg|thumb|left| Exit holes [http://biostor.org/reference/55262 © RJ Gagné]]]&lt;br /&gt;
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There are various mango gall flies:&lt;br /&gt;
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- Erosomyia mangifereae or Procontarinia frugivora has been observed in the Philippines, India, West Indies and Brazil.&lt;br /&gt;
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- Asynapta sp. in West Indies.&lt;br /&gt;
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- Dasyneura mangifereae in Hawaii.&lt;br /&gt;
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- P. allahabadensis, P. biharana, P. brunneigallicola, P. viridigallicola, P. echinogalliperda, P. keshopurensis, P. mangifoliae, P. tenuispatha, P. amraeomyia and Dasyneura amaramanjarae have been observed in India (and some in Pakistan).&lt;br /&gt;
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- P. mangicola in China and Japan.&lt;br /&gt;
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- P. matteiana (1.5 mm long) in India, Kenya, Mauritius, Java and Réunion[http://biostor.org/reference/55262][http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=4&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFEQFjAD&amp;amp;url=http%3A%2F%2Fwww.geocities.ws%2Fmdce2005%2FMDCE2005%2F27-98_102.pdf&amp;amp;ei=HS5zU-P3DomXyQOd_IHgCA&amp;amp;usg=AFQjCNHlZtUS8qYFE2lCVSqh3ow0K4ukLg&amp;amp;sig2=vW-1UFdjzeE3l58kmc8eMg&amp;amp;bvm=bv.66699033,d.bGQ]. &lt;br /&gt;
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The tiny (1-2 mm) mango gall flies lay their eggs on the surface of young leaves, buds, shoots and flowers. After hatching, the maggots will eat their way into the plant tissue. This will show as galls (3-4mm) on the leaves. Once mature, they drop to the ground to pupate. The holes that they left behind are susceptible to fungal infections. Infested fruits initially show small (1mm) brownish lesions, which grow as the fruit grows. In young fruits, the exit holes are usually near the point of attachment, on the bottom side. Most infested fruits fall to the ground before ripening. Optimal conditions for reproduction is 26°C and a relative humidity of 70 to 80%. Infestation particularly occurs at bud-burst stage, at fruit set and on tender leaves of new flushes.[http://openagricola.nal.usda.gov/Record/IND92003672]&lt;br /&gt;
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[u]Natural enemies[/u]; Parasitic insects such as Platygaster sp., Eupelmus sp., Tetrastichus sp., Systasis dasyneurae [http://www.cabdirect.org/abstracts/19881115612.html], Pirene sp. and the predator ants Formica rufa (aka red wood ant, native to Europe and North America), Oecophylla longinoda (weaver ant native to West and Central Africa) and Oecophylla smaragdina (native to South-east Asia) and Camponotus sp. (aka carpenter ant, native to forests world wide).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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[u]Baits[/u]; Coriander oil attracts Erosomyia. D. amaramanjarae is attracted by a mixture of glycine, sodium hydroxide, ammonium carbonate and strong ammonia solution. Bordeaux mixture is a repellent. &lt;br /&gt;
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[u]Chemicals[/u]; Various systemic insecticides are equally effective: Dimecron (phosphamidon), Anthio (formothion) and Metasystox (demeton-S-methyl). Most effective is a mixture of phosphamidon (0.03%) and diazinon (0.03%).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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==Mango Moths==&lt;br /&gt;
[[Image:Indarbela_quadrinotata.jpg|thumb|left| Indarbela quadrinotata [http://www.agriplaza.in/orange-protection.htm#indarbela © Agriplaza]]]&lt;br /&gt;
[[Image:Podagrionella_habitus.jpg|thumb|right| Podagrionella [http://www.waspweb.org/Chalcidoidea/Torymidae/Toryminae/Podagrionini/Podagrionella/index.htm © S. van Noort]]]&lt;br /&gt;
[[Image:Indarbela_quadrinotata_2.jpg|thumb|left| Indarbela quadrinotata [http://www.ku.ac.th/e-magazine/september43/bark_feeders/ © KUAC]]]&lt;br /&gt;
[[Image:Eudocima_materna.jpg|thumb|left| Eudocima materna ]]&lt;br /&gt;
[[Image:Mantis_religiosa_5.jpg|thumb|right| Mantid eating large moth [http://www.agefotostock.com/en/Stock-Images/Rights-Managed/K60-589612 © Oriol Cabrero]]]&lt;br /&gt;
[[Image:Eudocima_materna_2.JPG|thumb|left| Eudocima materna [http://www.indianaturewatch.net/displayimage.php?id=285084 © Sivakumar V K]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Eudocima_homaena_2.jpg|thumb|left| Eudocima homaena [http://gaga.biodiv.tw/9702bx/049.htm © GaGa]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Eudocima_homaena_4.jpg|thumb|left| Eudocima homaena [http://blog.xuite.net/mbbrgs/twblog1/137791571-%E9%91%B2%E8%90%BD%E8%91%89%E5%A4%9C%E8%9B%BE++Eudocima+homaena++(Hubner,+1823)]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-l5.php © PathPiva]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella_2.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-1.php © PathPiva]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Orthaga_exvinacea.jpg|thumb|left| Orthaga exvinacea [http://www.nbaii.res.in/insectpests/images/Orthaga-exvinacea7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Orthaga_euadrusalis.jpg|thumb|left| Orthaga euadrusalis [http://www.jpmoth.org/~dmoth/62_Pyralidae/6003_Epipaschiinae/60031001_Orthaga_euadrusalis_1865/Orthaga%20eudrusalis%200208287801.jpg]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_2.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42845]]]&lt;br /&gt;
[[Image:Aleiodes_indiscretus.jpg|thumb|right| Aleiodes indiscretus [http://commons.wikimedia.org/wiki/File:Aleiodes_indiscretus_wasp_parasitizing_gypsy_moth_caterpillar.jpg © Agricultural Research Service]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42848]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_3.jpg|thumb|left| Citripestis eutraphera [http://www.boldsystems.org/index.php/Taxbrowser_Taxonpage?taxid=375866 © ANIC/BIO]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_2.jpg|thumb|left| P. jocosatrix [http://www.nbaii.res.in/insectpests/images/Penicillaria-jocosatrix7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_3.jpg|thumb|left| P. jocosatrix [http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar © DAFF]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix.jpg|thumb|left| P. jocosatrix [http://www.flickr.com/photos/bettaman/3973171117/ ©]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Caterpillar.jpg|thumb|left| Deanolis sublimbalis [http://www.padil.gov.au/pests-and-diseases/pest/main/136263/43328 © S. Eyres]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Moth.jpg|thumb|left| Deanolis sublimbalis [http://www.ces.csiro.au/aicn/name_s/b_1295.htm © DAFF]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Chlumetia_transversa_2.jpg|thumb|left| C. transversa [http://www.nbaii.res.in/insectpests/Chlumetia-transversa.php © NBAII]]]&lt;br /&gt;
[[Image:Chlumetia_transversa.jpg|thumb|left| C. transversa [http://www.inaturalist.org/observations/572035 © T Bonebrake]]]&lt;br /&gt;
[[Image:Goryphus_basilaris.jpg|thumb|right| Goryphus basilaris [http://www.hkwildlife.net/viewthread.php?tid=49119 © Daydream]]]&lt;br /&gt;
[[Image:Parasa_lepida.jpg|thumb|left| P. lepida [http://fanseab.exblog.jp/9707580]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Parasa_lepida_2.jpg|thumb|left| P. lepida [http://www.isan.clubs.chula.ac.th/para_norkhai/?transaction=post_view.php&amp;amp;cat_main=all&amp;amp;id_main=301&amp;amp;star=270&amp;amp;pg=28 © ปิ่นลม]]]&lt;br /&gt;
[[Image:Parus_major.jpg|thumb|right| Parus major [http://en.wikipedia.org/wiki/Parus_major#mediaviewer/File:Parus_major_2_Luc_Viatour.jpg © Luc Viatour / www.Lucnix.be]]]&lt;br /&gt;
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[u]Bark Eating Caterpillar[/u]&lt;br /&gt;
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The Bark Eating Caterpillar (Indarbela quadrinotata and Indarbela tetraonis) aka Mango Bark Feeder has been observed in India, Bangladesh and Sri Lanka. The moth has a wingspan of maximally 2 cm. This wood boring insect may attack a wide variety of trees, including mango, guava, jujube, pomegranate and apple trees. In plantations, infestation generally starts with the onset of premonsoon rains. The main symptom is plastered dark-brown masses on tree trunks and large branches. These webbed masses consist of chewed on wood remainders / frass, excrements and silken thread. Eggs are laid in clusters on the bark of trees. Initially, the newly hatched larvae occur in groups browsing on the bark. Then they migrate and lodge in shoots and buds. As the larvae grow, they start tunneling into the wood, up to 25 cm deep. This tunnel is kept free of frass. The frass and excreta from the tunnels are used for constructing the extended tunnel mouth (sleeve). As the larvae get bigger, they make the tunnel wider. The larvae hide in these tunnels during the day and come out at night to eat the surrounding bark of the tree. The rate of feeding is faster during the hottest months. The larva minimally eats 16 mg / day, at 12.5 degrees Celsius. The maximum is 166 mg/day at 35 degrees Celsius. Similarly, food consumption is maximum at 96% RH and minimum at 56% RH.&lt;br /&gt;
When large patches of bark are eaten away, the tree gets increasingly exposed to various bacteria, fungi etc. And if substantial areas of bark have been eaten, the tree will eventually die. Prolonged water stress decreases the resistance of the host tree. The larvae pupate inside the tunnel and the emerging moth leaves its &amp;quot;pupal skin&amp;quot; at the mouth of the borer hole. The pupa is 1.5 cm long. The larval stage lasts for about 8 months. The pupal period lasts for about 9 days. It has a little more than one generation per year.  [http://www.tropecol.com/pdf/open/PDF_49_1/09%20Shashidharan.pdf]&lt;br /&gt;
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Natural enemies; Two fungi; Aspergillus candidus and Beauveria bassiana have been reported to cause mortality of the Bark Eating Caterpillar in the field. Laboratory trials have indicated 100% larval mortality by these fungi. The Bark Eating Caterpillar is parasitized by Podagrionella indarbelae (&amp;#039;metallic&amp;#039; parasitic wasps), which may be fed the eggs of I. tetraonis.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf] Podagrionella is found in tropical African countries, Senegal, Algeria, Malawi, France, Spain, India, Thailand, Indonesia, The Philippines, Australia&lt;br /&gt;
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Control; prevent overcrowding by sufficient spacing in between trees. One may insert a sharp metallic probe/spike into each tunnel/hole to kill the larvae. One may seal the tunnel entrance using tar or wax. Of the various insecticides screened against this insect, monocrotophos (0.1 %), quinalphos (0.1 %) and fenvalerate (0.08%) gave best results.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf]&lt;br /&gt;
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[u]Fruit-piercing Moths[/u] &lt;br /&gt;
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Eudocima (aka Othreis) are very large moths (up to 10 cm wingspan) with orange abdomen. The most widespread specie is [http://www.waiwiki.org/index.php?title=Eudocima_phalonia Eudocima fullonia] (aka Eudocima phalonia)[http://www.hindawi.com/journals/tswj/2011/753484/] Eudocima moths look like dead leaves when in hiding mode. Until they show their bright orange hindwings with broad black margins and a large black spot in the middle. Mature larvae are 4 to 5 cm long. These moths have been observed in virtually any tropical country, except for the Americas. The adults fly (very well), feed and mate during the night. In tropical countries, they live up to a month. The adults feed on fruit juice by puncturing the fruit. The resulting wound is used by various bugs and diseases to attack the fruit (including mango, banana, grapes, orange, clementine, kiwi, lychee, pineapple, peach, apricot, papaya apple and pear). Eggs are laid singly, or in masses (up to 750 in a cluster, which may be used for rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings]), on any part of the plant. In tropical conditions, eggs hatch within 4 days. Larvae may feed around the clock and mature in 3 weeks, after which they pupate in cocoons of silk-spun leaves. Pupation favors wet conditions and occurs in 12 to 18 days.[http://www.aphis.usda.gov/plant_health/plant_pest_info/pest_detection/downloads/pra/efulloniapra.pdf] Pupation is always in a roomy cell made of living leaves woven together with silk and slightly lined; the pupa is attached to this lining by the cremaster.[http://www.mothsofborneo.com/part-15-16/calpini/calpini_4.php] Temperatures below 16°C during the activity period after dusk prevents feeding, mating and oviposition.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] &lt;br /&gt;
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Their larvae do not feed on mangoes, nor the mango trees. Instead, the larvae feed on very specific Fabaceae and Menispermaceae. Variation in the alkaloids associated with certain menisperm genera (eg quaternary alkaloids in Tinospora[https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2007-969933]) may explain the very specific (or even exclusive) moth–host plant relationships.[http://www.publish.csiro.au/?paper=SB9960227] Eudocima larvae are known to feed extensively on Stephania japonica (Menispermaceae) and its varieties.[http://www.calodema.com/freefiles/412.pdf] In Australia the larvae of Eudocima salaminia feed almost exclusively on the forest vine, Stephania japonica.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] Eudocima materna feeds exclusively on Tinospora cordifolia [https://books.google.nl/books?id=UxVFMwEACAAJ&amp;amp;dq=inauthor:%22BHUMANNAVAR+BASAVARAJ+S%22&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=MHaIVL2mBMLwUvDcg5gF&amp;amp;ved=0CCIQ6AEwAA] (widespread in tropical regions [http://link.springer.com/article/10.1007/s00606-004-0293-1#page-1], including Africa[http://informahealthcare.com/doi/abs/10.1080/14756360802333075]).Eudocima homaena may feed on Quisqualis indica.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41], Achyranthes, Cocculus, Cyclea, Menispermum and Tiliacora species.[http://www.en.inforapid.org/index/index.php?search=Cocculus] Eudocima jordani and Eudocima fullonia may be generalist feeders.[http://www.researchgate.net/publication/249439696_Differential_habitat_affinities_of_five_species_of_fruitpiercing_moths_(Lepidoptera_Noctuidae)_in_their_utilization_of_Tinospora_smilacina_Benth._as_a_larval_host_plant_in_north_Queensland]&lt;br /&gt;
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Control; Regularly check the orchard during the night using a flashlight. The adult Eudocima moths have large eyes that glow red when illuminated. Adult [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] may eat large moths such as Fruit-piercing moths. The female Praying mantis lays a few hundred eggs in a cluster (ootheca) attached to an object like a branch or trunk. Large species of mantids may have a length of up to 12 cm. Their colour is usually adapted to the environment they naturally live in. They have about 2 generations per year. Young mantids look like adults, but don&amp;#039;t have wings. Adult females often have no wings either, or reduced wings.&lt;br /&gt;
Eudocima are parasitized by Euplectrus [http://link.springer.com/article/10.1023/B:BICO.0000036439.74117.2f#page-1][http://www.aapmhe.in/index.php/pmhe/article/view/42] and Trichogramma.[http://images.peabody.yale.edu/lepsoc/jls/2010s/2011/2011-65-1-053.pdf]&lt;br /&gt;
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[u]Honeydew Moth[/u]&lt;br /&gt;
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The Honeydew Moth (Cryptoblabes gnidiella aka Albinia casazzar aka Albinia wockiana) aka Christmasberry Moth survives only in warm climates (all over the world). It attacks a large variety of crops, including mangoes, banana, oranges, clementines, grapes, loquats, pomegranates, avocado, coffee, maize and rice. The caterpillars (1 cm long) mainly feed superficially on fruits, but may also feed on leaves, flower, shoots and bark. The newly hatched larvae and the adults, however, solely feed on honeydew. The adults are active during the night. Females mate 1 to 4 times during their entire life. One Honeydew Moth may lay up to 100 eggs within 2 days after mating, on fruit or leaves (averagely 150 in total, during lifetime). These eggs hatch within one week (or longer, in colder regions). It has several generations per year, depending on the climate. Adult moths are good flyers (1 to 2 cm wingspan) and may rapidly spread to poorly maintained orchards (infested with honeydew).&lt;br /&gt;
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Natural enemies; Scolothrips sexmaculatus and Orius spp. prey on the eggs and early-instar larvae. The Honeydew Moth is parasitized by Phanerotoma sp.[http://www.cabdirect.org/abstracts/19740514736.html] and Trichogramma Platneri (both parasitoid wasps).&lt;br /&gt;
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Trichogramma are extremely tiny wasps (&amp;lt; 0.5 mm). They insert their eggs into the eggs of the moth. The larvae feed, grow and pupate inside the host egg, which converts the colour of the host egg from white to black. The emerging wasp eats its way out and is ready to mate. The eggs from unmated females will produce only males, whereas the eggs from mated females will produce both males and females. Adult Trichogramma feed on nectar from flowers. One female wasp may parasitize 50 moth eggs during her life. The optimimum conditions for Trichogramma are 20 to 27 degrees Celsius and 60% RH. Most insecticides kill Trichogramma.&lt;br /&gt;
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Control; This moth is attracted by honeydew secreted by aphids, scale insects and other honeydew secreting bugs. It is therefore most effectively controlled by controlling all honeydew secreting bugs. Its instars are highly susceptible to Bacillus thuringiensis.&lt;br /&gt;
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[u]Leaf Webber[/u]&lt;br /&gt;
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These brown moths (Orthaga euadrusalis and Orthaga exvinacea) are a minor mango (and Cashew nut) pest. They have been observed in India and Indonesia. Females mate only one time during their whole life.[http://www.ncbi.nlm.nih.gov/pubmed/20136014] The leaf webber lays eggs on young leaves and buds and creates a web around pods, leaves and flowers for its larvae to pupate in. Such a webbed cluster may contain several larvae. The Leaf Webber causes relatively very little damage, as the larvae merely scrape and eat from the surface of the leaves.&lt;br /&gt;
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Control: Application of Serratia marcescens (a bacterium), Aspergillus flavus (a fungus) and Beauveria bassiana (a fungus) are all very effective.[http://www.cabdirect.org/abstracts/19810586732.html] The leaf webber is parasitized by Brachymeria lasus, Hormius sp., Pediobius bruchicida and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html] and also by Trichogramma chilonis and Trichogramma pretiosum.[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=37933]&lt;br /&gt;
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[u]Mango Fruit Borer[/u];&lt;br /&gt;
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The Mango fruit borer (Citripestis eutraphera; a moth) has been observed in Indonesia, India and Australia. Its lays its eggs (&amp;lt; 1 mm) on fruit or stalk. The larvae feed on mango pulp (and cashews) and cause premature fruit drop, mainly in young fruit. The larvae hatch in 2 days. They are initially white, then red-violet when young, turning to dark blue as they grow. Hatched larvae initially feed on the peel of the fruit. Then they bore into into the fruit and feed for 12 to 15 days before they pupate in the fallen fruit, or in the soil. Often there is more than one larva in a fruit with a total of 15 recorded in one fruit.[http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42846].&lt;br /&gt;
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Control: Its natural enemies are parasitoid wasps (Aleiodes sp. and Euplectrus sp.) and a parasitoid fly (Blepharella lateralis).&lt;br /&gt;
[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=9416] To protect the stems, cover them with a cloth or Jute and paste charcoal over it. Fostoxin tablets can also be placed and sealed in the holes made by the borers.&lt;br /&gt;
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[u]Mango Shoot Borer[/u]&lt;br /&gt;
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Mango Shoot Borer (Penicillaria jocosatrix) or Velvet mango slug; P. jocosatrix (wingspan 2 - 2.5 cm) has been observed in Indonesia, The Philippines, Malaysia, Vietnam, Guam, Hawaii, Thailand and Australia. Its larvae; the caterpillar of this moth (up to 3 cm long) causes damage to shoots, stems, inflorescence, flowers and fruits of the mango tree. Pestalotiopsis anacardiacearum sp. nov. (fungal leaf rot) is found on dead mango leaves associated with P. jocosatrix (the Mango Shoot Borer).[http://biotaxa.org/Phytotaxa/article/view/phytotaxa.99.2.1] Eggs hatch in 3-5 days. Larval development takes 8-10 days. Mature larvae pupate in the soil and the moth emerges 16-20 days later.[http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar]&lt;br /&gt;
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Natural enemies: Euplectrus sp. (a parasitoid wasp) and Blepharella lateralis (a parasitoid fly) may effectively decrease Mango shoot borer infestation [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], and are also effective against the Mango fruit borer.&lt;br /&gt;
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[u]Mango Seed Borer[/u]&lt;br /&gt;
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The Mango Seed Borer (Deanolis sublimbalis aka Autocharis albizonalis) is the Red banded mango caterpillar. It is a mango pest in Australia, Burma, The Philippines, Malaysia, Vietnam, China, Indonesia and Papua New Guinea. Severe infestation may cause up to 50% yield reduction. This grey snout moth (wingspan 2 cm) lays its eggs on the top of the mango. Its larvae (one or more per fruit) develop within the fruit in 2 to 3 weeks. Initially they feed on the pulp, and then on the seed. The fruit will split, rot and drop. The mature larvae will pupate in the soil.&lt;br /&gt;
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Natural enemies: Rychium attrisimum are wasps that feed on the larvae of the Mano Seed Borer as they leave the fruit.[http://www.planthealthaustralia.com.au/wp-content/uploads/2013/03/Red-banded-mango-caterpillar-CP.pdf] Trichogramma chilonis and Trichogramma chilotreae are tiny parasitoid wasps that parasitize the eggs of the Mango Seed Borer and hundreds of other moths. They are so small that with the naked eye you cannot see what they are. They have a wingspan of 0.5 mm. They live about 2 weeks, and may lay several eggs in a single moth egg.&lt;br /&gt;
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Chemicals: If you have no other option, Deltamethrin and Cyfluthrin are most effective.&lt;br /&gt;
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[u]Mango Tip Borer[/u]&lt;br /&gt;
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Mango Tip / Shoot / Bark / Trunk / Twig Borer (Chlumetia transversa) aka aka Shoot Boring Caterpillar is a serious mango (and litchi) pest. The major symptom is drying of the tip of young shoots. Female moths lay their eggs on tender leaves. Newly hatched larvae bore into these leaves. As they grow they bore into young shoots near the growing point, and subsequently tunnel down. The leaves of affected shoots will droop. It has four generations each year, and in colder regions overwinters as pupae in young branches and bark. Generations are overlapped.[http://europepmc.org/abstract/CBA/367089] Full grown caterpillars are 2 to 2.5 cm long.&lt;br /&gt;
C. transversa has been observed in Indonesia, The Philippines, Malaysia, Thailand, Taiwan, China, India, Pakistan, Sri Lanka and Australia. &lt;br /&gt;
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The Mango Tip Borer is parasitised by Bracon greeni, Meteorus sp. and Goryphus sp [http://www.cabdirect.org/abstracts/19810586735.html], which are all parasitoid wasps. Its larvae are also parasitized by the fly Megaselia chlumetiae sp. nov. Its larvae enter the host and develop by consuming the host&amp;#039;s internal tissues. Pupariation normally took place within the host&amp;#039;s empty skin.[http://www.cabdirect.org/abstracts/19921163681.html]&lt;br /&gt;
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Control; If you have no other option, spraying 2.5% deltamethrin EC for three times may be most effective (87% control efficiency) and the least harmful to the tree. Four insecticides tested; 80% dichlorvos EC(dilution of 1250), 47.5% chlorpyrifos EC(dilution of 1000), 2.5% deltamethrin EC (dilution of 2500) and 2% abamectin EC(dilution of 2000) were applied on mango trees during 2 months. Second best was 2% abamectin EC at a dilution of 2000. The other two insecticides were less effective, which might be due to the development of resistance in pests against these insecticides on account of longer repeated applications.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GXNY200910010.htm]&lt;br /&gt;
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[u]Nettle Caterpillar[/u]&lt;br /&gt;
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The Nettle Caterpillar, aka the Blue-striped Nettle Grub (Parasa lepida aka Latoia lepida; 2 to 3 cm long) has been observed in China, Pakistan, India, Sri Lanka, Bangladesh, Vietnam, Thailand, Malaysia, Japan and especially Indonesia. Parasa lepida may infest various fruit trees, including mango, cherry, persimmon, Indian almond and rose apple, and also coffee, tea, cocoa and palms, with a preference for coconut trees. The caterpillar of this species have spines containing a poison that causes serious irritation and inflammation in humans upon contact. It feeds on the leaves and shoots of the mango tree. Initially, defoliation will be localized, affecting only one or a few trees. Thus the infestation is easily identified. Subsequently, as new generations of this pest emerge, the infested area may rapidly expand. The female moth lays eggs on mango leaves. The cocoons are laid side by side. The cocoons are so hard and stout that they remain on the trunks for 5 to 6 years after spinning. Cocoons from which adults have successfully emerged have clear emergence holes. Newly hatched caterpillars will feed on the underside of the epidermis, stripping it off the leaflets. They often begin where the eggs were laid; at the tip. Then they eat the edges of the leaflet and eat large areas of the lamina. When they have finished developing, the whole leaflet will have been consumed systematically from tip to base, leaving only the midrib. On this midrib, the notched indentations are the only visible remainders left by the caterpillars. [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=38935]&lt;br /&gt;
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Parasa lepida is predated on by Agriosphodrus dohrni (The Assassin Bug, native to China, India, Indonesia). This 2 cm long Assasin Bug may be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
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The great tit (Parus major) is a predator of Parasa lepida cocoons in Japan. The most effective parasites are Apanteles parasae (a parasitoid wasp) and Chaetexorista javana (a parasitoid fly).[http://www.cabdirect.org/abstracts/19820596771.html]&lt;br /&gt;
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Salt spray from the sea and the resulting plant stress (considerable damage) may negatively affect the Parasa lepida moth.[http://www.bioone.org/doi/pdf/10.3956/0031-0603-83.3.193]&lt;br /&gt;
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==Mango Scales==&lt;br /&gt;
[[Image:Coccus_viridis.jpg|thumb|left| Coccus viridis [http://www.insectimages.org/browse/detail.cfm?imgnum=5385208 © J.W. Lotz]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| M. signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
[[Image:Coccus_hesperidum.jpg|thumb|left| Coccus hesperidum [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/09Cocc_hesperidum/4hesperidumHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Ceroplastes_floridensis.jpg|thumb|left| Ceroplastes floridensis [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/03Cero_floridensis/4floridensisHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Chilocorus_kuwanae.jpg|thumb|right| C. kuwanae [http://animal.memozee.com/view.php?tid=2&amp;amp;did=10472 © Jinsuk Kim]]]&lt;br /&gt;
[[Image:Aulacaspis_tubercularis.jpg|thumb|left| Aulacaspis tubercularis[http://gaga.biodiv.tw/new23/9409/076.htm]]]&lt;br /&gt;
[[Image:Azya_orbigera.jpg|thumb|right| A. orbigera [http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php © E Arcaya]]]&lt;br /&gt;
[[Image:Drosicha_mangiferae.jpg|thumb|left| D. mangiferae [http://www.jugantor.com/last-page/2014/04/19/89802 ©]]]&lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri.jpg|thumb|right| C. montrouzieri[http://www.ozanimals.com/Insect/Mealybug-Ladybird/Cryptolaemus/montrouzieri.html ©]]]&lt;br /&gt;
[[Image:Rastrococcus_iceryoides.jpg|thumb|left| R. iceryoides [http://www.nbaii.res.in/insectpests/Rastrococcus-iceryoides.php © NBAII]]] &lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri_larva.jpg|thumb|right| C. montrouzieri larva [http://www.insectimages.org/ © J.W. Lotz, FDACS Bugwood.org]]]&lt;br /&gt;
[[Image:Paracoccus_marginatus.jpg|thumb|left| Papaya mealybug [http://www.ablturismo.com/avispa-enviada-por-correo-salva-cultivos-en-la-india/ ©]]]&lt;br /&gt;
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The main mango scales are:&lt;br /&gt;
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* Coccids ; Coccus viridus (soft green scales) and Coccus hesperidum (soft brown scales)&lt;br /&gt;
* wax scales ; Ceroplastes spp.&lt;br /&gt;
* armoured scales ; Aulacaspis tubercularis, Chloropulvinaria polygonata (the Mango Green Shield Scale) and Aspidiotus destructor.&lt;br /&gt;
* Mealybugs (pseudococcidae) ; Drosicha mangiferae, Rastrococcus iceryoides (&amp;quot;Mango mealybugs&amp;quot;) and Paracoccus marginatus (&amp;quot;Papaya mealybug&amp;quot;, which is also a mango pest)&lt;br /&gt;
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Mango scales suck the sap from the leaves, and as a result, the leaves start drying. This may cause branches to die, blemished fruits and premature dropping. Scales are small insects (1 to 7 mm), generally immobile; as if shells are glued to the plant. Only the newly hatched crawlers (emerging from under a big scale) move to their feeding site. Soft scales produce honeydew, which may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould]. Armoured scales don&amp;#039;t excrete honeydew. The papaya mealybug has been observed in Mexico, Florida, Antigua, Cuba, Dominican Republic, Guadeloupe, Haiti, St Kitts, Martinique, Puerto Rico, St Martin, St Barthélémy, Virgin Islands.[http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=6&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEIQFjAF&amp;amp;url=http%3A%2F%2Fwww.cabi.org%2FISC%2FFullTextPDF%2F2013%2F20133231104.pdf&amp;amp;ei=VeZ8U_eFNaqX1AXh_YHYDA&amp;amp;usg=AFQjCNGWK-GGi3IRhAfSNBg6nah3XhoKWw&amp;amp;bvm=bv.67229260,d.d2k]&lt;br /&gt;
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[u]Natural enemies[/u]; Natural enemies of scales include parasitic wasps, ladybird beetles and lacewings, which are killed by broad-spectrum pesticides. Because of their honeydew secretion, scales may be protected / farmed by specific ants (eg arboreal ants (Azteca instabilis) farming soft green scales).[http://www.ncbi.nlm.nih.gov/pubmed/18559179] Some fungal pathogens effect this symbiotic interaction.[http://www.ncbi.nlm.nih.gov/pubmed/23905728] Parasitoid insects use the honeydew secreted by coccids (and aphids etc) as an infochemical to locate their hosts.[http://www.ncbi.nlm.nih.gov/pubmed/15112724] R. iceryoides is parasitized by Dinocarsis sp., Microterys flavus, Metastenus concinnus and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
Of the parasitoids Acerophagus papayae and Anagyrus loecki, A. papayae is the most effective in decimating the papaya mealybug. (Meyerdirk)&lt;br /&gt;
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[u]Lacewings[/u]; Most lacewings (also) feed on plant material, such as pollen. Particularly green lacewings such as Mallada boninensis, Mallada astur and Mallada signata are mainly predatory and very effective. They are relatively small (length up to 1.5 cm, including wings). Adults are active during the night, and may be attracted by flashlight. Eggs are attached to leaves and hatch within 4 days. The larval period lasts 12 days, and the pupal period lasts about 9 days. Adults live just one month. One may commercially rear millions of green lacewings for biological control of mango pests.&lt;br /&gt;
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[u]Cryptolaemus montrouzieri[/u] (aka Mealybug Ladybird) is a (4 mm long) predatory ladybird that eats mealybugs, soft scales (Coccidae) and armoured scales (Diaspididae). Originally from Australia, introduced in Florida and commercially available as a mealybug control agent. It needs warm, humid weather. Survival temperature range is 15-35°C. Life cycle on average is 30 days under optimum conditions: 23 °C and 60-70% humidity. Optimal temperature for adults is 28°C. They are active between 16°C and 33°C, and 70 to 80% RH. Longevity of females is 70 days max. It produces 4 generations per year. Development is accelerated at higher temperatures. At 25°C it may lay a total of 200 to 700 eggs (av. 400), at the rate of averagely 9 eggs per day. They preferrably lay their eggs in the egg mass of mealybugs. The eggs hatch in 5 to 6 days. There are 4 larval stages, and the entire larval period lasts about 12 to 17 days. At pupation stage they are about 13 mm long. Adults emerge after 7 to 10 days. These beetles prefer high concentrations of mealybugs to predate on, and will fly away when concentrations of mealybugs are lower.[http://www.arabscientist.org/english/page/11/] During its larval development, one C. montrouzieri may eat 2400 eggs of C. polygonata.[http://www.cabdirect.org/abstracts/19981112241.html] Unfortunately, the filaments produced by its larvae look very similar to mealybugs. Initially release 1 or 2 new active beetles per square meter in the morning, before it gets hot. Subsequently regularly release 2 to 3 beetles per tree. The beetles will be killed by pesticides, but tolerate copper-fungicides.&lt;br /&gt;
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[u]Chilocorus kuwanae[/u]; This ladybird beetle is a primary predator of wax scales (Ceroplastes sp.). This ladybird beetle is attracted by (spraying) methyl jasmonate, mainly due to the terpenoid compound alpha-pinene [http://www.ncbi.nlm.nih.gov/pubmed/19825299]&lt;br /&gt;
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[u]Microweisea coccidivora[/u]; a tiny (1mm long) predatory ladybird beetle from Florida and South Carolina (USA) that feeds particularly on armoured scales.&lt;br /&gt;
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[u]Azya orbigera[/u]; The larvae of this ladybird beetle predate on Coccus viridis. These larvae are relatively immune to ant attacks (ants that farm coccids), due to their sticky waxy filaments. Also, the presence of ants reduces A. orbigera larvae getting parasitized.[http://www.ncbi.nlm.nih.gov/pubmed/18348805] A. orbigera has been observed in Colombia, Guyana, Venezuela, Bélize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, México, USA, Trinidad and the West Indies.[http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php]&lt;br /&gt;
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[u]Sumnius cardoni[/u] (a predatory ladybird) predates on nymphs of D. mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19810586735.html] &lt;br /&gt;
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[u]Coccodiplosis sp[/u] (a parasitic gall midge)[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9], Cybocephalus sp. (a predatory beetle) and Scymnus coccivora (a predatory ladybird) predate on R. iceryoides (mealybug). &lt;br /&gt;
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[u]Cryptochetum sp.[/u] (parasitic flies) parasitize the 1st- and 2nd-instar nymphs of the Drosicha mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
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[u]Coccophagus scutellaris[/u] parasitizes Coccus hesperidum by laying eggs in its gut.[http://www.ncbi.nlm.nih.gov/pubmed/747455] When parasited and parasiteless hosts are available, the female of Coccophagus deposits more eggs on the latter.[http://www.ncbi.nlm.nih.gov/pubmed/7283545]&lt;br /&gt;
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[u]Thomsonisca pakistanensis[/u] is a parasitic wasp that most commonly parasitizes Aspidiotus destructor. A. destructor is also parasitized by Aneristus ceroplastae (tiny parasytic wasp), Comperiella bifasciata (parasytic wasp also effective against yellow scales; Aonidiella citrina[http://www.publish.csiro.au/paper/ZO9710053.htm]), Chartocerus sp. and Chrysonotomyia sp. (both also parasytic wasps) [http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
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[u]Aphytis sp[/u]; This tiny (2 to 3 mm long) parasytic wasp parasitizes Aulacaspis tubercularis (up to 46% parasitism in a test).[http://www.actahort.org/books/509/509_96.htm]&lt;br /&gt;
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[u]Lecanicillium lecanii[/u]; This fungus kills various coccids, including Coccus hesperidum.[http://www.ncbi.nlm.nih.gov/pubmed/20863711] Also Ceroplastes sp. may be killed by Lecanicillium lecanii. Adding body materials of life coccids to a multi-generation culture (medium), significantly keeps the vigor and higher virulence of this fungus.[http://www.ncbi.nlm.nih.gov/pubmed/20387464]&lt;br /&gt;
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[u]Control[/u]: Collect the affected leaves and burn them. Spraying chlorogenic acid (a phenol naturally present in coffee beans) stimulates locomotory activity of the green scale Coccus viridis, thus minimizing their feeding. [http://www.ncbi.nlm.nih.gov/pubmed/20857759]&lt;br /&gt;
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If you have no other option: Use Metasystox 25% EC at the rate of 0.3 L in 450 L water or Fotidal 50 EC at the rate of 0.5 L in 450 L water / acre. Or spray mineral oil at low concentrations (not damaging the trees) after fruit picking, but not during flowering. Don&amp;#039;t spray during droughts or excessive heat.&lt;br /&gt;
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==Weevils==&lt;br /&gt;
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[u]The Mango Stone Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_mangiferae.jpg|thumb|left| Mango Stone Weevil [http://en.wikipedia.org/wiki/Sternochetus_mangiferae#mediaviewer/File:Mango_seed_weevil_2.jpg © P.Jeganathan]]]&lt;br /&gt;
[[Image:Oecophylla_smaragdina.jpg|thumb|right| Stone Weevil captured by O. smaragdina [http://www.cabi.org/isc/datasheet/16434 © Renkang Peng]]]&lt;br /&gt;
[[Image:Mantis_religiosa.jpg|thumb|right| Praying mantis [http://www.statesymbolsusa.org/Connecticut/insect_praying_mantis.html © Andy Williams / CritterZone]]]&lt;br /&gt;
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The Stone Weevil (Sternochetus/Curculio/Rhynchaenus/Cryptorhynchus mangiferae) aka Mango Seed / Nut Weevil is a short weevil; 7 mm to 1 cm long and 4 mm wide. It possesses a tough exoskeleton. Weevils are a type of beetle. The scales of the Stone Weevil may be of various colours; shades of black, grey, red or yellow. The Stone Weevil has been observed in many mango-producing countries all over the world (including Ghana, Nigeria, Kenya, Mozambique, Tanzania, Uganda, Zambia, India, Bangladesh, Indonesia, Thailand, Malaysia, Australia, USA)[http://www.cabi.org/isc/datasheet/16434]. Adult weevils can hardly fly, and this pest spreads by fruit transports. At dusk and during the night, the adults feed on leaves and young shoots. The female lays her eggs on and just under the surface of the peel of young (about 3 cm long) mangoes. Each female can lay up to 15 eggs per day and may lay several eggs on one fruit. The Stone Weevil prefers the sweetest (high-sugar) varieties of mango. After laying an egg, the weevil will make an incision in the fruit to let the sap from the fruit cover the egg, and to make it stick to the fruit. The amber-coloured sap will harden out, and remain as a protective resin mark over the egg. Newly emerged grubs are white and slender, and have no legs. They bore their way through the pulp towards the seed, within 2 days. Larvae and pupae develop inside the fruit. Pupation occurs in the seed. No external symptoms of attack are readily visible on infested fruits. Once matured (which takes up to 2 months), they eat their way out. About 25% of adult the weevils over-winter in the seed. They can live 2 years. One generation is produced each year.&lt;br /&gt;
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Natural enemies; The Stone Weevil is effectively predated on by Weaver ants such as Oecophylla smaragdina and Oecophylla longinoda.[http://iiste.org/Journals/index.php/JBAH/article/view/12284] Weaver ants, however, may also farm (for the produced honeydew) and protect aphids and various mango scales against their natural enemies. &lt;br /&gt;
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Weevils are also predated on by [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids], which may eat anything they can catch, including flies, beetles and moths. Adults readily mate in captivity and have one generation per season. Rearing mantids requires rearing of other insects, such as aphids and drosophilae (for nymphs), tephritids (for young mantids) and moths (for adult mantids) in large quantities. Females lay their eggs in a sticky cluster of several cm long. Nymphs (very tiny praying mantids) emerge from eggs. The developing nymphs need to be separated and fed aphids/drosophilae, or they will become cannibalistic.&lt;br /&gt;
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Control; Collect and destroy all fallen fruits, seeds, leaves, twigs etc. If you have no other option, spraying Fenthion (0.01%) on the trees at the time of oviposition is found to be effective. Fenthion, however, is extremely toxic to beneficial insects [http://www.actahort.org/books/509/509_96.htm], so that applying Fenthion may lead to secondary infestation of Mealybugs. A single spray of tiametoksam (Actara™ SC 240g/ℓ a.i.) at the roots during flower set may be effective for seasonal control.[http://etd.uovs.ac.za/ETD-db/theses/available/etd-09182009-083002/unrestricted/LouwCE.pdf] In Alphonso and Bagan Pali mangoes, a single spray (at dusk) of Monocrotophos 36EC 1ml per 1 litre of water was found to be very effective (97.5% to 100% mortality) [http://issuu.com/kisanadmin/docs/mango1/16] In a laboratory test, Beauveria bassiana was somewhat effective (30% mortality in 14 days), but in an orchard setting there was no effect at all.[http://www.cabdirect.org/abstracts/19971101160.html]&lt;br /&gt;
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[u]The Mango Pulp Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_frigidus.jpg|thumb|left| Mango Pulp Weevil [http://www.thaibugs.com/?page_id=284 © Thaibugs]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
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The Mango Pulp Weevil (Sternochetus frigidus or Cryptorrhynchus gravis) has been observed in Bangladesh, India, Indonesia, Malaysia, Myanmar,&lt;br /&gt;
Pakistan, Papua New Guinea, Philippines, Singapore and Thailand. This female weevil lays eggs (25-60 eggs / week) on mango fruits (minimum 6 cm diameter). Newly hatched larvae tunnel into the fruit pulp. The larvae form a chamber (constructed of frass) next to the seed, from which they eat their way through the pulp. Pupation occurs within these chambers. Matured weevils leave the ripe fruit through an exit hole in the peel. Prior to this exit, nothing shows that the fruit is infected. They are fully matured after 6 weeks; able to mate, repeatedly. One complete life cycle varies from 34-35 days. More than half of adult weevils hibernate in seeds. Mango Pulp Weevils are very poor flyers (maximum 90 cm horizontally).[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Thrips==&lt;br /&gt;
[[Image:Frankliniella_occidentalis.jpg|thumb|left| F. occidentalis [http://conabio.inaturalist.org/taxa/243761-Frankliniella © Th0th]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Thrips, or thunderflies, thunderbugs, storm flies, thunderblights, storm bugs, corn flies or corn lice may feed on many plants. Frankliniella bispinosa, Frankliniella kelliae (Florida, Caribbeans) and Frankliniella occidentalis (California, Israel) also feed on mango. They feed on young leaves, shoots and flowers, causing discoloration and deformation.&lt;br /&gt;
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Thrips are tiny (1 mm long on average; varying from 0.5 mm to 1.4 cm). They are not good flyers, but may readily be carried by the wind. Given the right conditions, they may rapidly multiply and form large swarms. To inspect trees for thrips infestation, just hold a bucket and shake a branch, and some thrips will fall off the infested branch into the bucket.&lt;br /&gt;
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Control: Natural enemies of thrips are Green lacewings (eg Mallada boninensis, Mallada signata), Orius insidiosus, Anystis agilis and Hypoaspis aculifer. Biological insecticides such as Beauveria bassiana and Verticillium lecanii may be effective.&lt;br /&gt;
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[u]Orius insidiosus[/u] is a 3 mm long pirate bug (aka insidious flower bug). They feed on thrips, mites, small caterpillars and various insect (including aphids&amp;#039;) eggs and larvae, and also pollen. They prefer eating thrips over aphids.[http://www.ncbi.nlm.nih.gov/pubmed/18845007] Orius may kill several thrips per day, beyond their need for nutrients. Adults fly well, and may readily disperse, looking for prey. O. insidiosus may be mass reared on [http://www.waiwiki.org/index.php?title=Armyworm armyworms]. They may occasionally sting people, which may hurt, but is completely harmless. Eggs are laid and embedded in the plant tissue of the fruit, stem, leave stalks and big veins at the underside of leaves. From eggs to adults, Orius goes through 5 larval stages. This takes about 16 to 18 days at 25°C. They are predatory at each stage (except as pupae). Adults live 3 to 4 weeks. As Orius albidipennis lays more eggs and has a shorter lifecycle, it may be a better biocontrol agent than Orius insidiosus.[http://eurekamag.com/research/030/621/030621437.php] Release at 2 to 3 Orius per square meter.&lt;br /&gt;
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==Whiteflies==&lt;br /&gt;
[[Image:Trialeurodes_vaporariorum.jpg|thumb|left| Trialeurodes vaporariorum [http://en.wikipedia.org/wiki/File:Weisse-Fliege.jpg © Gaucho]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| Orius insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Paraleyrodes_bondar.jpg|thumb|left| Paraleyrodes bondar [http://manateeornprod.wordpress.com/2012/02/22/new-whitefly-in-florida/ © UF/IFAS]]]&lt;br /&gt;
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Whiteflies (incl. Aleurodicus rugioperculatus, Aleurodicus dispersus, Aleurothrixus floccosus, Aleyrodes proletella, Bemisia tabaci, Paraleyrodes bondar, Siphoninus phillyreae, Trialeurodes vaporariorum) attack various crops, including mango, orange, tomato and watermelon. &lt;br /&gt;
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Whiteflies suck the saps from leaves, causing direct damage. They are also vectors of various diseases, including viruses. Additionally they may produce a lot of honeydew, which may lead to sooty mould. The density of the whitefly is positively correlated with maximum temperature and negatively correlated with relative humidity.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Natural enemies[/u]; Various animals predate on whiteflies, including green lacewings, Orius insidiosus and ladybird beetles. The minute parasitic wasps Encarsia guadeloupae and Encarsia haitiensi very effectively cause reduction in the population of Aleurodicus despersus.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Control[/u]&lt;br /&gt;
Dissolve 1 kg of sugar per 1 L of water. Poor in plastic transparent bottle. Drill holes in the upper part (above water level); small enough for whiteflies to enter, but not for bees. Put the lid on. Hang 2 to 3 bottles in each tree at flower set. Renew weekly.&lt;br /&gt;
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=Integrated Pest Management=&lt;br /&gt;
Integrated Pest Management (IPM) integrates proper orchard managment (such as sufficient spacing of trees, pruning off overlapping branches, the use of bait traps and removing affected fruits and plant parts) and the use of a variety of anti-bugs (natural enemies of mango bugs), so that they are complementary and sufficiently reduce all pest populations, minimizing the use of pesticides.&lt;br /&gt;
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Below is an example of the complementary use of anti-bugs, for both predation and parasitism.&lt;br /&gt;
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Preliminary requirements: A sealed, ventilated room. Constant supply of mangoes, or grapes, kiwi, lychee, pineapple, peach, apricot, papaya, apple or pear, to feed and rear Drosophilae (2-4mm long &amp;#039;fruit flies&amp;#039;), Tephritids (4-7 mm long &amp;#039;true&amp;#039; fruit flies) and Eudocima sp. (fruit piercing moths; up to 10 cm wingspan). One section needs daylight exposure, for growing Fabacae / Menispermacae for the Eudocima sp. to lay their eggs, and for their larvae to feed on the leaves. This sealed room should rather be too large than too small to prevent a lack of food for the reared predators. Any surplus of eggs and/or larvae may be sold as fish feed.&lt;br /&gt;
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* Rearing [http://www.waiwiki.org/index.php?title=Praying_mantis Praying Mantids] to predate on moths, caterpillars, weevils and young Longicorn beetles by adults, and on fruit flies, hoppers and midgets by young mantids. These mantids may be reared on fruit flies  (Drosophila and Tephretids) and specific moths. Young mantids should be separated after hatching, in boxes. Nymphs should be fed Drosophilae. Young mantids should be fed Tephretids. Adult mantids are fed Eudocima moths or Armyworm moths.&lt;br /&gt;
* Rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings] (Mallada signata, Mallada boninensis) to predate on aphids, scales, hoppers and thrips in the field. These lacewings may be reared on Eudocima sp. eggs collected from the Fabacae / Menispermacae in the sealed room, or on Armyworm eggs. &lt;br /&gt;
* Rearing the parasitic wasps Fopius arisanus and/or Diachasmimorpha longicaudata to parasitize Tephritids (fruit flies). Tephritid eggs should be collected from fruits in the sealed room. To prevent the fruit fly eggs from drying out, the eggs should be kept on a moist substrate. To prevent molding, a constant air current should be provided during the time required for parasitoid development.&lt;br /&gt;
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The second phase may be more labour intensive. To rear Tetrastichus sp. and Euplectrus sp. or Trichogramma sp. for the purpose of parasitizing scales, moths, midges and longicorn beetles, one most collect eggs from all species in the field. Instead, one may also rear subject pests.&lt;br /&gt;
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As an alternative for green lacewings, one may use the combination of Cryptolaemus montrouzieri (mass reared on Plancoccus citri on potatoes or squash [http://www.arabscientist.org/english/page/11/], for predating on scales) and Orius insidiosus (mass reared on [http://www.waiwiki.org/index.php?title=Armyworm Armyworms] (which are best reared on Bermudagrass), for predating on aphids, thrips and small caterpillars). This allowes for a more targeted approach (if there is only 1 pest, eg scales). This may alsobe a better combination (together with Praying mantids) because adult Praying mantids prefer to predate on lacewings (15 mm long) over the much smaller C. montrouzieri (4 mm) and O. orius (3 mm).&lt;br /&gt;
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=Diseases=&lt;br /&gt;
Bugs cause damage to the mango tree, which makes the tree more susceptible to disease. Many bugs are also disease vectors; they transport the viruses, fungi etc. to the mango tree. Successfully combatting mango bugs, is therefore key to prevention and control of mango diseases. Using chemicals to fight mango diseases and/or bugs, however, may actually result in killing natural enemies of the bugs that spread disease.&lt;br /&gt;
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==Anthracnose==&lt;br /&gt;
[[Image:Anthracnose.jpg|thumb|left| Anthracnose [http://www.indiancropdiseases.com/mango.aspx]]]&lt;br /&gt;
[[Image:Anthracnose-2.jpg|thumb|right| Anthracnose [http://hawaiiplantdisease.net/cpg/displayimage.php?pid=397 © Nelson]]]&lt;br /&gt;
Anthracnose is caused by the fungi Colletotrichum acutatum [http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2003.93.5.579] and Glomerella cingulata [http://www.idosi.org/wjas/wjas4(6)/8.pdf][http://www.jofamericanscience.org/journals/am-sci/am0608/46_3350am0608_361_367.pdf]. It particularly affects young shoots, flowers and fruits. The disease is stimulated by high humidity, frequent rains or mists, and a temperature range of 24 to 32°C. The results of this disease are black spots on panicles, leaf spot, blossom blight, withered tip, twig blight and fruit rot. Particulary young and tender shoots and foliage are affected, causing die back of young branches. Wounded twigs may also be infected and may sometimes die off. Fruits develop black spots and rotting and young fruits may shrivel and prematurely drop. If the entire inflorescence is destroyed, no fruits will set. &lt;br /&gt;
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[u]Where?[/u] It widely occurs in the orchard and in storage, Anthracnose has been reported in Argentina, Florida, Hawaii, India, Pakistan, Philippines, Sri Lanka, South Africa and Trinidad.&lt;br /&gt;
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[u]Control[/u]: Burn all fallen leaves. Prune affected twigs and burn them. Apply Bordeaux paste on cut ends. To prevent blossom infection, spray twice at 15 days interval during flowering with Carbendazirn (Bavistin 0.1%). To prevent foliar infection, spray copper fungicides (0.3%) twice a week. Apply a teaspoon of dish detergent per spray load to make the copper sulfate stick. Prevent the copper sulfate from dripping on the ground to prevent contamination of the soil and the roots of the tree.&lt;br /&gt;
Picked mangoes should be submerged for 15 minutes in hot water (52°C) with Carbendazim (0.1%). One may also dip the mangoes in 500 ppm Benomyl and 900 ppm Thiobendazole. C. acutatum is also effectively inhibited by the synthetic strigolactone GR24.[http://www.ncbi.nlm.nih.gov/pubmed/21688170]&lt;br /&gt;
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==Bacterial Canker==&lt;br /&gt;
Bacterial black spot (bacterial canker) is a bacterial disease caused by Xanthomonas campestris pv. mangiferaeindicae. It affects many varieties. Initially, irregular small water soaked lesions will appear on the leaves. These will extend into patches of dead tissue. The leaves will turn yellow and die. The lesions will also appear on the the stalk of the leaves, twigs and fruits. The fruits will then turn brown and black, and burst open. The released fluid is filled with bacteria that will contaminate the rest of the tree or other fruits in storage.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Agrimycin-100 (0.01%) or Streptocycline (0.01%), 3 times at 10 days interval. Subsequently spray Copper Oxychloride (0.3%) or Carbendazim (Bavistin 0.1%) once a month.&lt;br /&gt;
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==Die Back==&lt;br /&gt;
Die back (Botryodiplodia (Lasiodiplodia) theobromae aka Physalospora disrupta/quercuum/rhodina/glandicola) may occur any time of the year. It is characterized by advancing discoloration and darkening of the bark. It extends along the veins of the leaves, causing browning and upwards rolling of the margins. Eventually the leaves shrivel and fall. Twigs and branches dry and defoliate, and may drain a yellow-brown gummy fluid. It may look as if the tree has been exposed to a bush fire.&lt;br /&gt;
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[u]Control[/u]: Prune diseased twigs 10 cm below the affected area. Paste the cut ends with Copper Oxychloride (0.3%) and also spray Copper Oxychloride (0.3%) on affected trees.&lt;br /&gt;
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==Diplodia Stem-end Rot==&lt;br /&gt;
This fungus (Lasiodiplodia theobromae) may enter the mango where the skin or stem has been injured mechanically. It will form a black circle around the pedicel.&lt;br /&gt;
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[u]Control[/u]: Prevent mechanical injuries. After picking, submerge mangoes for 15 minutes in hot water (53°C) with Carbendazirn (0.1%).&lt;br /&gt;
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==Mango Black Blight==&lt;br /&gt;
Black blight is caused by a fungus (Capnodium mangiferum).&lt;br /&gt;
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==Mango Blight==&lt;br /&gt;
Mango blight is caused by Erwinia bacteria (Enterobacteriaceae). Many spots appear on the leaves which cause a reduction in growth and yield. &lt;br /&gt;
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[u]Control[/u]: Use Dithane M 45 at the rate of 1.7g/L (450 L water per acre).&lt;br /&gt;
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==Mango Malformation==&lt;br /&gt;
Mango malformation (Fusarium mangiferae) has been reported in Australia, Bangladesh, Brazil, Central America, Cuba, Egypt, India, Israel, Malaysia, Mexico, Pakistan, South Africa, Sudan, Switzerland, UAE and the USA. It is a disease caused by fusarium species [http://www.mycologia.org/content/94/4/722.full][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.6.456] (F. subglutinans from Egypt, Florida, Israel, Malaysia and South Africa, F. sterilihyphosum from Brazil and South Africa, and Fusarium sp. nov. and F. proliferatum from Malaysia)[http://www.ncbi.nlm.nih.gov/pubmed/18943188][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-96-0667 Free Full Text]. The disease is stimulated by relatively cool conditions; 10 to 27°C, and merely survive hotter conditions. In this disease the leaves and inflorescence of the mango tree are badly deformed and gradually dry up. There is no fruit setting and hence no production. &lt;br /&gt;
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[u]Control[/u]: Various fungicides, including Captan, Benomyl and Thiram have been reported to be effective to some extend. &lt;br /&gt;
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==Mango Scabs==&lt;br /&gt;
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Mango Scabs (Denticularia mangiferae aka Sphaceloma mangiferae aka Elsinoë mangiferae) survives on young tissues (young leaves, shoots, flower, fruits) of the mango tree only. Adult tissue is not susceptible. Its spores (and their germination) requires rain splash and free water to produce new infections.[http://www.cerambycoidea.com/titles/affa2004.pdf] Moisture conditions stimulate its growth. Though this parasite is not a fungus but in a persistent parasytic relationship with the mango tree, it produces symptoms that look somewhat familiar to those of anthracnose (a fungus) infections. It starts with small light- or dark brown or grey spots on the underside of leaves and fruit. These spots get bigger and darker with time. In the center of this lesion a cracked texture will appear. If the host tissue survives, lesions may develop into scar tissue. When infestation is severe, there is loss of leaves and fruit.&lt;br /&gt;
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[u]Control[/u]; Remove and destroy all fallen leaves, twigs, branches etc. Spray copper hydroxide or copper oxychloride on a very regular basis, particularly in the rainy season. Without chemical control, losses as high as 90% have been observed.[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Phoma Blight==&lt;br /&gt;
Phoma blight (Phoma glomerata) is a fungus that affects the fibre in the top layer of old leaves. It produces small, irregular, angular lesions. As the lesions grown, the colour changes from yellow-brown to cinnamon. Many small spots may form overwhelming patches and result defoliation of affected leaves. Phoma glomerate very effectively produces an enzyme that converts the remainders (glucose) of fibre (from the leaves) into it the main structural component (N-acetyl-D-glucosamine)[http://www.ncbi.nlm.nih.gov/pubmed/15553782] in its cell walls, thus enabling its own growth.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Benomyl (0.2%), and 20 days later spray 0.3% Miltox (Copper Oxychloride plus Zineb). Quadris (azoxystrobin) combined with thymol at a non-fungitoxic concentration produces much higher growth inhibition of Phoma glomerata than the fungicide alone.[http://www.ncbi.nlm.nih.gov/pubmed/22408641]&lt;br /&gt;
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==Powdery Mildew==&lt;br /&gt;
Powdery mildew (Oidium mangiferae) affects virtually all mango varieties. Characteristically, a white superficial powdery fungal grows on the inflorescence and tender leaves, stalk of panicles, flowers and young fruits. The affected flowers and fruits will pre-maturely drop, or fruit setting does not occur at all. The disease is initiated and developed by warm (&amp;gt;17°C) dry weather, but during the flowering stimulated by prolonged rains or mists and by cool nights. The disease has been reported in Brazil, India, Jamaica, Pakistan, Sri Lanka, South Africa and the U.S.A. &lt;br /&gt;
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[u]Control[/u]: Alternately spray wettable sulphur (0.2% ; 2 g Sulfex/L), Tridemorph (O.1% ; 1 ml Calixin/L) and Bavistin (0.1%) at 15 days interval. Start spraying when the panicle (flower cluster) starts to emerge. Or spray with Carbendazim (0.1%) or Tridemefon (0.05%) or wettable sulphur (0.3%) alone (3 days in a row).&lt;br /&gt;
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[u]Control-2[/u]: Similar to A. quisqualis AQ10 Biofungicide in commercial use for biocontrol of powdery mildew, Phoma glomerata (see [http://www.waiwiki.org/index.php?title=Mango#Phoma_Blight Phoma Blight] above) may act as mycoparasite of powdery mildew. Phoma glomerata can colonize and suppress development of powdery mildew.[http://www.ncbi.nlm.nih.gov/pubmed/10618259] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91841/ Full free text]&lt;br /&gt;
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==Red Rust==&lt;br /&gt;
Red rust (Cephaleuros virescens aka C. parasiticus) initially causes round and slightly elevated green-grey spots mainly on leaves. They turn into rusty red spots and may form bigger patches. This reduces the photosynthetic capacity of the leaves. In severely infected trees, twigs will remain stunted, the bark and twigs will get thick and the leaves will dry up and drop off. &lt;br /&gt;
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[u]Control[/u]: Spray Copper Oxychloride (0.3%) 3 times.&lt;br /&gt;
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==Sooty Mould==&lt;br /&gt;
Sooty mould (Meliola mangiferae) may be the result of too many insects in your orchard. Scale insects, mealy bug and hoppers secrete honey dew. The honey dew sticks to the leaves, feeding fungal growth. This results in a black sooty mould over the entire surface of leaves and twigs, affecting the photosynthetic capacity of the leaves. Uncontrolled, the tree may completely turn black.&lt;br /&gt;
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[u]Control[/u]: Prune and burn the affected branches. Spray Wettasulf (0.2%) with+ Metacid (0.1 %) and gum acacia (0.3%). Ants feed on the honeydew excreted by soft scales, preventing accumulation of sooty moulds, but they also protect the scales from natural enemies.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
	</entry>
	<entry>
		<id>http://www.waiwiki.org/index.php?title=Mango&amp;diff=4225</id>
		<title>Mango</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Mango&amp;diff=4225"/>
		<updated>2014-12-11T19:44:06Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling, Spacing, Notation&lt;/p&gt;
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&lt;div&gt;Comparable to oranges and clementines, mangoes are among the most all-round fruits, regarding nutrients. Mangoes are cultivated in (sub)tropical regions all over the world.&lt;br /&gt;
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=Low-fibre, sweet mango species=&lt;br /&gt;
If you use a juice extractor like the [http://www.thekitchn.com/product-review-hurom-slow-juic-131492 Hurom] / [http://versapers.fr/ Versapers] (masticating slow juicer), the stringy fibres from species like Tommy Atkins or Haden will clog your juicer (more so than pineapples). The chamber will fill up with long fibres that simply never get discarded, so that you need to empty and clean it after every few mangoes. With mangoes like Kent (#11 on the list), you may endlessly (&amp;gt;100 mangoes) continue juicing without having to empty or clean the chamber in between, because the short fibres are immediately discarded as pulp (as with apples, pears, clementines).&lt;br /&gt;
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==Anwar Ratol==&lt;br /&gt;
[[Image:Anwar_Ratol.jpg|thumb|right| Anwar Ratol [http://freshfruitpakistan.blogspot.nl/2013/04/anwar-ratol-top-variety-of-mango.html © H. Khalid]]]&lt;br /&gt;
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* #1 (aka Anwer Rathol, Anwar Retaul or Anwar Rataul)&lt;br /&gt;
* Small size (av. 180 g), medium thick skin&lt;br /&gt;
* Matures from green to pale bright yellow &lt;br /&gt;
* Very sweet (TSS 26-28%)&lt;br /&gt;
* Fibreless flesh, medium juicy&lt;br /&gt;
* Originally from Rataul, in Bagpat district, in the province of Uttar Pradesh, India. &lt;br /&gt;
* Harvested from June to July (India and Punjab in Pakistan)&lt;br /&gt;
* Keeps well in storage&lt;br /&gt;
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==Alphonso==&lt;br /&gt;
[[Image:Alphonso.jpg|thumb|right|]]&lt;br /&gt;
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* #2 (aka Alphanso, Bombay)&lt;br /&gt;
* Small, roundish / oblong (av. 200 g.)&lt;br /&gt;
* Smooth, (from green to) pale greenish-yellow to deep orange peel (deepening with ripening)&lt;br /&gt;
* Smooth, firm flesh. Intense reddish-orange colour&lt;br /&gt;
* Sweet, somewhat sour (17% sugar; sugar/acid ratio: 39; TSS 21-23%) and flavoured&lt;br /&gt;
* Virtually without fibre (1%), medium juicy&lt;br /&gt;
* Originally from Western India&lt;br /&gt;
* Main production in Maharashtra and Gujarat&lt;br /&gt;
* Highest quality in Sindhudurg, Ratnagiri (Natwarlal plantation), Raigad and Thane districts&lt;br /&gt;
* Harvested from early April to early July (India) and July (some districts in Sindh, Pakistan)&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
* Tolerates high humidity&lt;br /&gt;
* Good keeping quality (up to 4 weeks at 11° C)&lt;br /&gt;
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==Dashehari==&lt;br /&gt;
[[Image:Dashehari.jpg|thumb|right|]]&lt;br /&gt;
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* #3 (aka Dasheri, Doshehri, Aman Dusehri or Dussehri)&lt;br /&gt;
* Small, oblong (av. 175 g)&lt;br /&gt;
* Matures from light green to light yellow, thin skin&lt;br /&gt;
* Sweet (TSS 21-22%), lemon yellow flesh&lt;br /&gt;
* Fibreless, medium juicy&lt;br /&gt;
* Very small stone&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Dasheri, Uttar Pradesh, India.&lt;br /&gt;
* Harvested from early June to July, mainly Malihabad, Uttar Pradesh (and Punjab)&lt;br /&gt;
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==Bagan Pali==&lt;br /&gt;
[[Image:Bagan_Pali.jpg|thumb|right|]]&lt;br /&gt;
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* #4 (aka Begun Palli, Began Phali, Banganapalli or Baganpali)&lt;br /&gt;
* Large (av. 550 g.), oval&lt;br /&gt;
* Sweet (TSS 18-20%)&lt;br /&gt;
* Fibreless, little juicy, yellow flesh &lt;br /&gt;
* Matures from dark green to yellow light green or yellow-orange&lt;br /&gt;
* Thin, smooth, shiny skin&lt;br /&gt;
* Originally from Sindh, Pakistan and/or Banganapalle in Andhra Pradesh, India.&lt;br /&gt;
* Harvested from July to August (Pakistan)&lt;br /&gt;
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==Sindhri==&lt;br /&gt;
[[Image:Sindhri.jpg|thumb|right|]]&lt;br /&gt;
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* #5 (aka Sindhry)&lt;br /&gt;
* Medium to large (av. 300 g., av. 400 g in Pakistan)&lt;br /&gt;
* Elongated with pointy curve&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 36; TSS 15-17%), flavoured&lt;br /&gt;
* Little fibre (3% - 5%), little juicy&lt;br /&gt;
* From green to deep matt lemon yellow thin peel (somewhat wrinkly) when ripe&lt;br /&gt;
* Deep yellow-orange flesh; soft and melting&lt;br /&gt;
* Originally from Mir Pur Khaas in Sindh&lt;br /&gt;
* Harvested from late May to July (Pakistan)&lt;br /&gt;
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==Chaunsa==&lt;br /&gt;
[[Image:Chaunsa.jpg|thumb|right|]]&lt;br /&gt;
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* #6 (aka Sammar Bahisht Chausa)&lt;br /&gt;
* Large (av. 350 g.), oblong&lt;br /&gt;
* Sweet (18% sugar; sugar/acid ratio: 58; TSS 18-20%)&lt;br /&gt;
* Soft, succulent flesh &lt;br /&gt;
* Medium fibre, large stone, medium juicy (delicious for eating, too stringy for the slow juicer)&lt;br /&gt;
* Thin, pale, matt yellow-greenish peel (wrinkly and slightly deeper when ripe)&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Pakistan&lt;br /&gt;
* Harvested mainly in the Rahim Yar Khan and Multan (Sahiwal) districts of Punjab in Pakistan&lt;br /&gt;
* Harvested from June to early September (Pakistan)&lt;br /&gt;
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==Maya==&lt;br /&gt;
&lt;br /&gt;
* #7&lt;br /&gt;
* Medium, round (av. 300 g.)&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 42; TSS 16-17%) and flavoured&lt;br /&gt;
* Red blush peel (with yellow tinge when ripe)&lt;br /&gt;
* Smooth, deep/pale yellow flesh (soft when ripe)&lt;br /&gt;
* Medium fibre, very juicy&lt;br /&gt;
* Harvested from mid-June to early July (Gambia)&lt;br /&gt;
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==Gopalbogh==&lt;br /&gt;
&lt;br /&gt;
* #8 (aka Gopal Bhogue)&lt;br /&gt;
* Small size (average 190 g.)&lt;br /&gt;
* Yellow-green peel, with small seeds&lt;br /&gt;
* Virtually without fibre&lt;br /&gt;
* Very sweet (21% sugar)&lt;br /&gt;
* Good quality mainly grown in Rajshahi region, especially in Chapainawabganj district (Bangladesh).&lt;br /&gt;
* Harvest from mid May to mid June (Bangladesh)&lt;br /&gt;
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==Sweet Elena==&lt;br /&gt;
[[Image:Sweet_Elena.jpg|thumb|right|]]&lt;br /&gt;
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* #9&lt;br /&gt;
* Large sized, ovoid (av. 350 g.)&lt;br /&gt;
* Thin, orange peel&lt;br /&gt;
* Yellow to orange flesh&lt;br /&gt;
* Little fibre&lt;br /&gt;
* Sweet (19% sugar)&lt;br /&gt;
* Originally (as a carabao variety) from Sta. Cruz, Zambales (Philippines)&lt;br /&gt;
* Harvest from March to April (Locloc, Palauig in northern Zambales, Philippines)&lt;br /&gt;
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==Ataulfo==&lt;br /&gt;
[[Image:Ataulfo.jpg|thumb|right|]]&lt;br /&gt;
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* #10 (aka Adaulfo, Adolfo, Champagne, Manila or honey mango, related to Alphonso)&lt;br /&gt;
* Small, oblong shape (av. 225 g.)&lt;br /&gt;
* With ripening, peel turns from pale green to gold-yellow (yellow to orange and wrinkles when fully ripe)&lt;br /&gt;
* Deep yellow flesh (gold when ripe), little fibre and thin pit&lt;br /&gt;
* Smooth, sweet (15% sugar), and buttery / creamy flesh&lt;br /&gt;
* Spicy flavour &lt;br /&gt;
* Best temperature is 28°C, tolerates 1100 to 3000 mm annual rainfall &lt;br /&gt;
* Originally from Chiapas, Mexico&lt;br /&gt;
* Harvest from mid-October to late December (Ecuador); February to August (Ghana)&lt;br /&gt;
* May be stored in the fridge for 2 weeks&lt;br /&gt;
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==Kent==&lt;br /&gt;
[[Image:Kent.jpg|thumb|right|]]&lt;br /&gt;
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* #11&lt;br /&gt;
* Large, rounded oval (500 to 800 g.)&lt;br /&gt;
* Smooth, waxy thick peel (green with some yellow and red; yellow increases with ripening)&lt;br /&gt;
* Soft, melting, juicy flesh (from deep yellow to deep orange when fully ripe)&lt;br /&gt;
* Limited fibre (dry weight: 0.4 - 2%), small seed&lt;br /&gt;
* Sweet (13% sugar; 14ºBrix [http://www.m.elewa.org/JAPS/2013/17.3/5.pdf])&lt;br /&gt;
* Originally from Mexico / Florida&lt;br /&gt;
* Harvest from January to March and May to August (Ghana)&lt;br /&gt;
* Large tree (up to 20 m.)&lt;br /&gt;
* No storage keeping below 13°C&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
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=Mango Cultivation=&lt;br /&gt;
==Conditions==&lt;br /&gt;
The best soil for mango trees has a pH between 5.5 and 7.5 (high pH may cause leaf tip burn). The temperature range for growing mangoes is 15 to 40°C. Mango does best within a temperature range of 24 to 27°C. The Indian race is in general intolerant of humidity (susceptible to anthracnose), the Philippine race tolerates excess moisture. The life span of each tree is approximately 100 years. Economic bearing life is 30 to 50 years.&lt;br /&gt;
Mango trees need deep soil (up to 2 m) for their root systems. Grafted saplings yield a more superior quality of fruit. Fruits are borne by mango trees from 4 to 5 years after planting. Full bearing starts at 6 to 7 years.&lt;br /&gt;
Grafted mango plants start bearing blossoms one to two years after planting, provided that there is no high humidity or intense rain during the flowering period (dryness induces flowering).&lt;br /&gt;
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==Rootstock Propagation==&lt;br /&gt;
[[Image:Mango_grafting.jpg|thumb|left| [http://www.muftisays.com/blog/ummi+taalib/2718_20-03-2012/shaikhmurid-relationship.html © ummi taalib]]] &lt;br /&gt;
[[Image:Grafted_seedling.jpg|thumb|centre| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
[[Image:Grafted_seedlings.jpg|thumb|right| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
Trees grown from mango seeds (and naturally, sexually propagated) will be very different from the tree those mangoes came from (such &amp;#039;wild mangoes&amp;#039; usually taste bad). That is why rootstocks (raised from seeds) are asexually propagated. Matured seedlings (about 60 cm tall) are grafted by inarching; a bud from a tree producing good quality mangoes is artificially attached to the rootstock. This will create a new tree with two parts: the lower (wild) part and the upper part, which is an exact copy of the tree you took this bud from. Any bud growing from the lower part needs to be pruned off immediately. The upper part needs to grow and produce branches, and eventually fruits. During the first month after planting, the young trees need to be irrigated every 3 days. During the next 2 months, they need to be irrigated once a week. After that they should not be watered for a couple of months. Each year they need a dry season of a few months. Young plants need 10 to 30 kg organic manure per plant. Don&amp;#039;t use a chemical fertilizer on young plants. Once the tree is 2 years old, you may start using a controlled release fertilizer (with higher nitrogen), like 12-5-9 or 12-8-34 (= ratios N:P:K); 1 tbsp. in 1 L warm water / meter tree height. Or just manure.  &lt;br /&gt;
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Rootstock branches should always be pruned off. To prevent viral infections, only prune using a very sharp, sterilized knife (sterilize on the spot with a propane torch).&lt;br /&gt;
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==Production technology== &lt;br /&gt;
Space mango trees about 12 meters apart. Adult mango trees need about 500-750 mm of water per year. In the wet season irrigated once per 8 to 10 days. In the dry season once per 15 to 20 days. Mango trees need dry and wet spells alternately (also kills various pathogens). Don&amp;#039;t fertilize mature trees during fruit bearing, and not too much irrigation. If during the blooming season the soil contains much nutrients (fertilizer) and water, the tree will grow, but not flower and bear fruits. &lt;br /&gt;
You may either:&lt;br /&gt;
* A: Apply 80 to 100 kg manure per year (eg humanure from [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets]), after all fruits are picked. Or:&lt;br /&gt;
* B: Apply 3-4 kg SSP, 2-3 kg Potassium Sulphate and 2-3 kg Urea before flowering. Plus 2-3 kg Urea in 2 doses after fruit setting. Or:&lt;br /&gt;
* C: Use a controlled release fertilizer once a year, such as 4-4-8 (= ratios N:P:K), after all fruits are picked (1 tbsp. in 1 L warm water / meter tree height).&lt;br /&gt;
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After fruit harvest, prune off diseased, dried, broken branches and those touching the ground. Every 3 to 4 years about 15 to 20% of old wood should be removed.&lt;br /&gt;
Picking should be done when the fruit is fully developed and mature. Natural drop of the fruit is the main indication that the fruit is ready for picking. Expected yields vary from 40 to 100 kg per tree.&lt;br /&gt;
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One cannot rear any animals (except for carnivores) on land with mango trees, as mango leaves are deadly poisonous.&lt;br /&gt;
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=Bugs=&lt;br /&gt;
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==Aphids==&lt;br /&gt;
[[Image:Propylea_quatuordecimpunctata.jpg|thumb|right| Predatory ladybird [http://www.colpolon.biol.uni.wroc.pl/propylea%20quatuordecimpunctata.htm]]]&lt;br /&gt;
[[Image:Aphids_on_mango.png|thumb|left| Aphids [http://www.infonet-biovision.org/default/ct/124/crops#_1804_1202 © Varela]]]&lt;br /&gt;
[[Image:Mallada_sp_2.jpg|thumb|right| Mallada sp. © Texas University]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
&lt;br /&gt;
Aphids (plant lice; Toxoptera odinae) are small (1-4 mm), living in clusters. They pierce plant tissue and suck the sap of the leaves, which may cause the leaves to bend, twist or roll up. Aphids also transmit plant viruses. Some species produce winged offspring, &amp;quot;alates&amp;quot;, that may readily disperse. Dry (and warm) weather stimulates increases in aphid numbers. Some species of ants (eg Dolichoderus cuspidatus and Formica aerate) protect and &amp;#039;farm&amp;#039; aphids, feeding on the honeydew released by the aphids&amp;#039; alimentary (gut) canals. Insects secreting honeydew may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould] &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Plant flowering plants at the boarders to attract beneficial insects. Plant &amp;#039;trap crops&amp;#039; (dill, nasturtiums, timothy grass) to monitor aphid numbers. Check the trap crops every 3 days. Infested trap crops need to be burned. Aphid&amp;#039;s natural enemies include predatory ladybirds (eg Propylea quatuordecimpunctata), hooverfly larvae, parasitic wasps, aphid midge larvae, crab spiders (Thomisidae) and lacewings (Neuroptera). Exposing aphid populations to natural predators may be successful particularly when its hot, because once bitten they release an alarm pheromone and the others all jump off the plant. Jumping aphids may experience a heat shock (&amp;gt; 35°C) since the ground may be much warmer than the shady tree. This heat shock usually sterilizes the aphids (killing the bacteria on aphids that provide nutrients essential for aphid reproduction). [http://www.sciencedaily.com/releases/2007/04/070419172046.htm]&lt;br /&gt;
&lt;br /&gt;
[u]Crab spiders (Thomisidae)[/u]; Crab spiders are ambush hunters (they don&amp;#039;t build webs) that not just eat aphids, but may also hunt ants that farm aphids. Some of these crab spiders, Xysticus sp, may however not just eat ants, but also predatory beetles (that eat aphids).&lt;br /&gt;
&lt;br /&gt;
[u]Green lacewings (Chrysopidae)[/u]; One may buy millions of lacewings (as captive-bred eggs) for biological pest control, as reared for that purpose in many countries. A female lacewing may plant over 100 eggs on plants infested with aphids. These eggs hang on a thin 1 cm long stalk, most often under a leaf. The larvae of most species of lacewings are specialised predators that eat aphids (and coccids such as mango scales, and caterpillars). These larvae sway their heads from left to right, and back, until they strike something they can eat. They have mouthparts that can pierce and suck the aphids. Many (adult) lacewing species also feed on nectar and pollen, but particularly Chrysopidae (eg Mallada signata, Mallada boninensis) are mainly predatory (one adult may eat 15 aphids per day). One may attract Chrysopidae by planting crops that attract them (mainly Asteraceae and Apiaceae), such as calliopsis (Coreopsis), cosmos (Cosmos), sunflowers (Helianthus), dandelion (Taraxacum), dill (Anethum) and angelica (Angelica). &lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option and immediate action is required: Use Folido 50% EC at the rate of 0.45 L per 450 L water / acre. (also kills natural aphid enemies)&lt;br /&gt;
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==Fruit Flies==&lt;br /&gt;
[[Image:Fruit_fly_maggots.jpg|thumb|centre| Maggots [http://www.infonet-biovision.org/default/ct/124/crops © A. M. Varela]]]&lt;br /&gt;
[[Image:Bactrocera_invadens.jpg|thumb|left| B. invadens [https://www.flickr.com/photos/iaea_imagebank/6776806998/ © IAEA]]]&lt;br /&gt;
[[Image:Fopius_arisanus.jpg|thumb|right| F. arisanus [http://www.tephritid.com/digital.php?act=page&amp;amp;pid=28&amp;amp;id=25 © tephritid.com]]]&lt;br /&gt;
[[Image:Ceratitis_capitata.jpg|thumb|left| C. capitata [http://josedotinzulza.edublogs.org/2013/04/21/how-much-damage-can-make-a-fly/ © José Inzulza]]]&lt;br /&gt;
[[Image:Diachasmimorpha_longicaudata.jpg|thumb|right| D. longicaudata [http://www.oocities.org/brisbane_parawasps/FruitFlyParasitoid.htm]]]&lt;br /&gt;
[[Image:Ceratitis_fasciventris.jpg|thumb|left| C. fasciventris [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Oecophylla_longinoda.jpg|thumb|right| O. longinoda [http://www.alexanderwild.com/Ants/Taxonomic-List-of-Ant-Genera/Oecophylla/i-tMfJXKG © A.Wild]]]&lt;br /&gt;
[[Image:Ceratitis_cosyra.jpg|thumb|left| C. cosyra [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Jackson_trap.jpg|thumb|right| Jackson trap]]&lt;br /&gt;
[[Image:Ceratitis_rosa.jpg|thumb|left| C. rosa [http://wzciq.wenzhou.gov.cn/art/2011/1/12/art_6811_63595.html © wzciq]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis eating fly [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
In 2013, the export of mangoes from Ghana and 27 other African countries was banned from major international markets mainly due to fruit fly infestation. The European Union imposed a ban from May 1 2014, on import of mangoes from India, also due to fruit fly infestation. &lt;br /&gt;
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The fruit flies (aka peacock flies; 4-7 mm long) that may form a very severe mango cultivation pest are not the Drosophilae (2-4 mm long), but the colourful Tephritidae family (&amp;gt; 5000 species). These attack mango fruits throughout the season and lay their eggs right under the (affected) skin of mature green or ripe fruits. They have three generations and multiply very rapidly. Within one or two days the eggs hatch into white maggots. The maggots feed on the fruit, which starts to rot. After 4 to 17 days, the maggots make holes in the skin and leave the fruit to pupate. &lt;br /&gt;
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The main fruit fly pests of mango in Africa are Ceratitis capitata (Medfly), C. fasciventris, C. rosa and C. cosyra (the most destructive). C. cosyra is however rapidly displaced by Bactrocera invadens (endemic to Sri Lanka)[http://www.ncbi.nlm.nih.gov/pubmed/19610411][http://www.ncbi.nlm.nih.gov/pubmed/22251685], being predominantly a low-land pest.[http://www.ncbi.nlm.nih.gov/pubmed/16923206] B. invadens populations quickly increase with the onset of the raining season[http://www.ncbi.nlm.nih.gov/pubmed/19449630] and also lay aggs in young fruits. Fruit fly populations peak in the late dry season.[http://www.ncbi.nlm.nih.gov/pubmed/17598527]&lt;br /&gt;
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[u]Control[/u]: Collect all the fallen and affected fruits and bury them deep (&amp;gt;50 cm) into the soil. Or you can use them to breed maggots in a sealed room for fish feed. Natural enemies of tephritids include Diapriidae (tiny wasps; &amp;lt;8mm), Braconidae (also parasitoid wasps) and Oecophylla longinoda (Latreille; a weaver ant). &lt;br /&gt;
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C. cosyra, but particularly C. fasciventris and Medfly may be effectively controlled by using the host-marking pheromone.[http://www.ncbi.nlm.nih.gov/pubmed/23356072] B. invadens [http://www.ncbi.nlm.nih.gov/pubmed/24293246], the Mediterranean fruit fly (C. capitata) and the Mexican fruit fly (Anastrepha ludens) may be effectively controlled by the &amp;#039;sterile insect technique&amp;#039; (releasing overwhelming numbers of sterile insects). The effects of sterile flies are greater than those for parasitoid wasps, but they are complementary.[http://www.ncbi.nlm.nih.gov/pubmed/15568340] &lt;br /&gt;
Medfly eggs and instars (right under the peel) may be killed by immersing Ataulfo mangoes for 95 min in warm water at 47°C, also positively modifying pH (producing more palatable fruits), but can also produce a loss of firmness and weight (5%).[http://www.ncbi.nlm.nih.gov/pubmed/23356057] B. invadens is no more heat tolerant than Medfly.[http://www.ncbi.nlm.nih.gov/pubmed/21404834] &lt;br /&gt;
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[u]Bait traps[/u]; &lt;br /&gt;
Pheromone traps (85% methyl-eugenol, 5% Naled (insecticide), 10% saturated sugar) can be used for trapping male flies (Jackson trap). A three-component attractant (ammonium acetate, putrescine, and trimethylamine lures) may be used to trap female population of Medfly. (No wind: 28 m. range).[http://www.ncbi.nlm.nih.gov/pubmed/21061993] Malathion-bait sprays and Phloxine B (a xanthene dye; D&amp;amp;C Red #28) are equally effective.[http://www.ncbi.nlm.nih.gov/pubmed/11777044]&lt;br /&gt;
Of six commercial food-based attractants, Nulure captured the greatest proportion of females: 74% compared with 51-68%. But Mazoferm E802 (with or without Spinosad) and Torula yeast captured 2.4-2.6 times more females and 3.4-4.0 times more males than Nulure (also more effective than GF-120, Hymlure and Biolure).[http://www.ncbi.nlm.nih.gov/pubmed/24665714]&lt;br /&gt;
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[u]Fopius arisanus (Sonan)[/u]; F. arisanus is a tiny but most effective[http://www.ncbi.nlm.nih.gov/pubmed/12241567] and the most dominant parasitoid wasp. As parasitism increases, fruit fly infestation decreases.[http://www.ncbi.nlm.nih.gov/pubmed/17598524] Of 3 commonly used bait sprays, which all suppress fruit fly populations, Spinosad and Phloxine B bait sprays are less harmful to these wasps than Malathion bait spray. [http://www.ncbi.nlm.nih.gov/pubmed/11561838]   &lt;br /&gt;
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[u]Diachasmimorpha longicaudata[/u]; D. longicaudata (longtailed fruit fly wasp) is the most important parasitoid wasp used as part of integrated pest management programs against fruit flies such as B. invadens and Ceratitis species. They lay one or more (when hosts are scarse) eggs in fruit fly larva.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014816/] Introduction of D. longicaudata poses minimal competitive risk to F. arisanus.[http://www.ncbi.nlm.nih.gov/pubmed/12241567]&lt;br /&gt;
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[u]Oecophylla longinoda[/u]; O. longinoda is a predator weaver ant that may eat whatever insect or bug they may catch, including caterpillars, beatles, honey bees, driver ants (Dorylus nigricans Ill.) and larvae. O. longinoda is a natural enemy of fruit flies as it eats their larvae. This weaver ant is fiercely territorial and will keep other species of ants (like aphids-farming black ants) at bay. The ants continuously patrol the tree that they live in and may also catch egg-laying female fruit flies in the act, and eat them. B. invadens and C. spp numbers on Kent mangoes may be greatly reduced by Oecophylla longinoda.[http://www.ncbi.nlm.nih.gov/pubmed/17598527] &lt;br /&gt;
Weaver ants, however, also feed on honeydew. They may farm a wide range of honeydew-producing Homoptera (bugs with sucking mouthparts, including scales, aphids and mealybugs), but are mostly associated with Coccids (Saissetia; no virus vector). Only when other Coccids are scarce or unavailable, O. longinoda may farm aphids and mealybugs (unarmoured scale insects that are virus vectors). Mainly in small trees and shrubs, the mechanical damage caused by Coccids is related to the size of the attendant ant colony. Dense populations of O. longinoda are supported by interplanting with clove.[http://www.cabdirect.org/abstracts/19540500227.html;jsessionid=36A454B839F0DEEADFA83F99A7206E6D]&lt;br /&gt;
For the protection of their harvested honeydew, these weaver ants weave a translucent silken tent around living leaves. They use the silk produced by their larvae. It is claimed that pheromones produced by the weaver ants repel egglaying fruit flies. A study in Senegal, however, found that pheromones produced by O. longinoda did hardly repel egglaying females of B. invadens.[http://www.ddrn.dk/filer/forum/File/Abstract_Christina_Abel.pdf]&lt;br /&gt;
On the other hand, trees colonized by O. longinoda (in Ghana) had only 6 to 10% fly infestation, compared to 3% fly infestation in trees treated with Cypermethrin plus Dimethoate. (1614 mg per tree)[http://ugspace.ug.edu.gh/handle/123456789/2387] &lt;br /&gt;
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[u]Praying mantids[/u] may also catch and eat fruit flies. Praying mantids are opportunist predators and do not farm any insects or other bugs. Particularly young mantids may not yet be too large to prey on fruit flies (tephredids). The younger and/or smaller the praying mantids are, the smaller the insects (and any other living creature they may catch) they prey on. Praying mantid nymphs initially feed on aphids and fruit flies (drosophila), and as they grow, they skip to larger preys, such as tephritids, and eventually moths and even much bigger preys.&lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option, GF-120 (Dow chemical) may greatly (81-89% after 7-10 weeks) reduce the number of B. invadens pupae per kg mango.[http://www.ncbi.nlm.nih.gov/pubmed/19449630] Or use Dioptries 80% at the rate of 1 L in 450 L water or Malathion 57% at the rate of 0.5 L to 450 L water / acre. Broad-spectrum insecticides are most damaging to fruit flies&amp;#039; natural enemies such as parasitoid wasps.[http://www.ncbi.nlm.nih.gov/pubmed/19886446] &lt;br /&gt;
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==Long-horn Beetles==&lt;br /&gt;
[[Image:Stem_borer.jpg|thumb|left| Stem Borer [http://www.bitterrootrestoration.com/mango/stem-borer-batocera-rufomaculata.html ©]]]&lt;br /&gt;
[[Image:Dastarcus_helophoroides.jpg|thumb|right| D. helophoroides[https://www.ffpri.affrc.go.jp/labs/seibut/bcg/bcg00284.html ©]]]&lt;br /&gt;
[[Image:Batocera_rufomaculata_2.jpg|thumb|left| Batocera rufomaculata [http://agritech.tnau.ac.in/crop_protection/crop_prot_crop_insectpest%20_Mango_pest&amp;amp;disease.html © TNAU]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Batocera_rubus.jpg|thumb|left| Batocera rubus [http://163.20.112.34/~afu/77v.htm © ]]]&lt;br /&gt;
[[Image:Batocera_baby.jpg|thumb|left| baby Batocera [https://www.flickr.com/photos/bondingtool/14291758294 © Samantha - thebondingtool.com]]]&lt;br /&gt;
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* [u]The (red-spotted) Stem Borer (Batocera rufomaculata)[/u], is a large long-horned beetle (up to 5 cm long body). It cuts incisions on the bark and lays its eggs (up to 200) inside the cuts or cracks of the (damaged) tree bark, trunk or cavities (eg exposed roots). The beetles feed on the bark of living trees and eat the green of young shoots. The grub of the stem borer pupates and feeds inside the stem of mango trees (and other fruit trees, including durian, figs, avocado, jackfruit and cashew nuts), creating a tunnel towards the surface (up to 2 cm in diameter), eventually becoming a beetle. Sap (from the tree) and frass (or coarse sawdust, from the larvae) may drip from the holes. Older larvae may also tunnel deeper in the trunk or branches. This causes drying of branches, and the tree may die. The incidence of infestation of trunk borer is highly influenced by maximum temperature. Infestation is highest when temperature and humidity are high.[http://www.ncbi.nlm.nih.gov/pubmed/24498801] For reproduction, it needs to lay its eggs in a stem that is at least 7 cm in diameter. The Stem Borer has been observed in the west coast of Africa, Madagascar, Mauritius, Réunion, Seychelles, Turkey, Israel, Syria, Iraq, Iran, Pakistan, India, Bangladesh, Sri Lanka, China, Nepal, Thailand, Indonesia, Malaysia and the West Indies. Adults may live up to 4 months. Full grown larva may be 8 to 10 cm long, and may live up to a year, causing a lot of damage. Larval and prepupal + pupal stage lasts about 280 and 24-29 days.[http://www.actahort.org/books/787/787_41.htm]&lt;br /&gt;
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* [u]The (yellow-spotted) Mango Longhorned Beetle (Batocera rubus)[/u] is also a wood borer and about equally large as the Stem Borer. Full grown larvae are 6 to 8 cm long. It attacks various fruit trees, including mango, breadfruit and figs, but also bonsay trees, rubber trees and freshly felled timber. Batocera rubus has been observed in Pakistan, India, Bangladesh, China, Cambodia, Indonesia, Malaysia, Phillipines, Thailand, Taiwan and Vietnam.&lt;br /&gt;
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[u]Natural enemies[/u]; Tiny parasitoid wasps such as Oobius agrili sp.n. (1 mm long), Spathius agrili, Avetianella batocera, Tetrastichus planipennisi and Avetianella xystrocerae sp.n (1.5 mm long) have been shown effective against (the larvae of) some specific wood-boring beetles (Agrilus planipennis, Agrilus sexsignatus and Xystrocera globosa)[http://www.emeraldashborer.info/files/Zhang%20et%20al%202005%20Oobius_Leah.pdf][http://www.fs.fed.us/nrs/pubs/other/2013/2013_McManus_FHTET-13-01.pdf]. Batocera rufus (the mango longhorn beetle), is parasitized (its eggs) by  Louricia ovivora, gen. et sp. n.(a moth), and Ooencyrtus batocerae, sp. n.(a wasp) [http://cabdirect.org/abstracts/19360501471.html;jsessionid=A9E2E64A372836E8E24F2B4E97287DC9] Oobius batocera is an opportunistic parasytic wasp that parasitizes various insect eggs, including beetle eggs. [http://www.nhm.ac.uk/research-curation/research/projects/chalcidoids/database/synonyms.dsml?FamilyCode=EE&amp;amp;ValFamTrib=&amp;amp;VALGENUS=Oobius&amp;amp;VALSPECIES=batocerae&amp;amp;VALAUTHOR=(Ferri%E8re)&amp;amp;VALDATE=1936&amp;amp;ValidAuthBracket=false&amp;amp;HOMCODE=0&amp;amp;&amp;amp;listPageURL=listChalcids.dsml%3FSuperfamily%3DChalcidoidea%26Family%3DEncyrtidae%26Genus%3DOobius] &lt;br /&gt;
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There are also various mites that parasitize Batocera longhorned beetles.[http://www.jstor.org/discover/10.2307/3225250?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21103843600301] Tetrapolipus diastocerae n. sp., Tetrapolipus afrobatocerae n. sp., Tetrapolipus ramarajui n. sp. and Tetrapolipus seemani n. sp. are described. Tetrapolipus afrobatocerae has been observed in Africa, Tetrapolipus hunteri in Australia.[http://www.researchgate.net/publication/233099056_The_Genus_Tetrapolipus_(Acari_Podapolipidae)_Parasites_of_Tropical_and_Semitropical_Cerambycidae_(Coleoptera)_New_Distribution_Records_and_Descriptions_of_Four_New_Species] Tetrapolipus sulawesiensis is a mite that parasitizes Batocera herculus.[http://d.wanfangdata.com.cn/periodical_dwfl200202010.aspx] &lt;br /&gt;
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[u]Dastarcus helophoroides[/u]; This predator/parasitoid beetle (originally from China and Japan) is an important natural enemy of longhorned beetles. In the larval stage they attack various long horn beetles, including Batocera horsfieldi and Anoplophora glabripennis. Populations are attracted specifically by the frass of their original host. Eggs are laid twice a year, near the host entrance hole, frass-extrusion holes or larval tunnel walls, guided by semiochemicals. The freshly hatched (at about 21°C) first instars (0.7 mm in length) have legs, and actively move to the host. They bite and paralyze the host, and then suck hemolymph and degenerated tissues from the dead or paralyzed prey. Adults can live over 4 years. [http://link.springer.com/article/10.1007%2Fs10526-009-9224-y#page-1] Mass production is feasible if hatched larvae are fed cerambycid larvae until they are about 8 mm in lentgh, and subsequently reared on artificial diet (silkworm pupa powder, dry yeasts, yeast extract, sucrose, peptone, squid oiver oil, preservatives and distilled water). The full grown larvae are about 18 mm in length.[http://link.springer.com/article/10.1023%2FA%3A1009936609401#page-1] Adults don&amp;#039;t readily disperse; they need to be released on each tree separately. And even if you release them at 1.2 m height, their effectiveness decreases with increasing height in the tree. Yet their effectiveness may be very high; up to 85% host mortality. [https://www.ffpri.affrc.go.jp/labs/kanko/401-1.pdf]&lt;br /&gt;
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[u]Control[/u]; The stem borer is active during the night and attracted by light, so that light traps may be effective. Bacillus thuringiensis is a bacterium commonly used as a biological pesticide, and naturally present in the gut of various catterpillars. This bacterium is toxic to many insects. A specific strain of this bacterium, Bacillus thuringiensis ZQ-89 is also toxic to adult long-horned beetles.[http://www.ncbi.nlm.nih.gov/pubmed/21332894] Prevent the stem borer from laying eggs in the bark by applying a thick layer of coaltar on the stem. Or one may paint (at 0.5%) adult mango trees with monocrotophos or phosalone.[http://www.cabdirect.org/abstracts/19790379463.html] One may also specifically apply the insectisides to the potentially affected sites (cracks and cavities), and to twigs and young shoots to deter feeding by adults. One may poke with a hard wire to physically damage, and/or inject insectisides where larvae are suspected. Chloroform, ethyl acetate, Metasystox [demeton-S-methyl] and a mixture of petroleum and kerosene oil (at 5 ml/bore) and ethylene dibromide (at 3 ml/bore) gave 100% mortality of B. rufomaculata larvae.[http://www.cabdirect.org/abstracts/19881107545.html] Plaster the holes with wet clay, aluminium phosphide tablets (3 g/hole) and dichlorvos (0.1% spray).[http://www.cabdirect.org/abstracts/19790379463.html] Among the chemical treatments, Imidacloprid 17.8% SL, Thiamethoxame 25% WG was found best in management of mango stem borer.[http://www.gifre.org/admin/papers/gjbahs/management-vol-2-4-gjbahs.pdf]&lt;br /&gt;
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==Mango Hoppers==&lt;br /&gt;
[[Image:Idioscopus_niveosparsus.jpg|thumb|left| I. niveosparsus [http://163.20.112.34/~afu/641x.htm ©]]]&lt;br /&gt;
[[Image:Mallada_basalis.jpg|thumb|right| Mallada sp. [http://3insect.blogspot.nl/2012/06/mallada-basalis.html © Chen KunTsan]]]&lt;br /&gt;
[[Image:Idioscopus_clypealis.jpg|thumb|left| I. clypealis [http://163.20.112.34/~afu/641y.htm ©]]]&lt;br /&gt;
[[Image:Mallada_signata_3.jpg|thumb|right| Mallada signata [http://www.oocities.org/brisbane_lacewings/Chrysopidae.htm © Keith Power]]]&lt;br /&gt;
[[Image:Idioscopus_nitidilus.jpg|thumb|left| I. nitidilus [http://tech.groups.yahoo.com/group/pestnet/message/6414 ©]]]&lt;br /&gt;
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Mango hoppers:&lt;br /&gt;
* Idioscoynio chypeabis&lt;br /&gt;
* Idioscopus niveosparsus&lt;br /&gt;
* Idioscopus clypealis&lt;br /&gt;
* Idioscopus nitidulus&lt;br /&gt;
* Amritodus atkinsoni aka mango leafhopper&lt;br /&gt;
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Mango hopper nymphs as well as adults puncture and suck the sap of tender parts of the mango plant. Heavy drainage will cause the leaves to curl and dry. Infested flowers will shrivel and turn brown. Hoppers also secrete honeydew, which may cause sooty mould, inhibiting the leaves from obtaining energy from daylight. Sufficient spacing of trees and pruning of overlapping branches are essential in preventing hopper infestation, as shade (and high humidity) favour hopper multiplication.&lt;br /&gt;
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[u]Natural enemies[/u]:&lt;br /&gt;
Parasites: Aprostocetus sp., Gonatocerus sp. and Polynema sp. parasitize eggs of Idioscopus clypealis and Idioscopus nitidulus.[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
A preparation of the fungus Beauveria bassiana is also somewhat effective against mango hoppers. Chrysopa lacciperda [Plesiochrysa lacciperda] and Mallada boninensis predate on on nymphs of I. clypealis, I. nitidulus and Amritodus atkinsoni.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9]&lt;br /&gt;
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[u]Mallada boninensis[/u]; This green lacewing is an effective [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=5&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFQQFjAE&amp;amp;url=http%3A%2F%2Fwww.researchjournal.co.in%2Fonline%2FIJAS%2FIJAS%25203(2)%2F3_A-164-166.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNGZ9IidKvjO6s1zNs0HvbUMpKKPBg] generalist predator, feeding on mealy bugs (Mani and Krishnamoorthi, 1987), white flies (Selvakumaran et aI., 1996), bollworms and aphids (Kabissa et al., 1996) and on nymphs of Aphis gossypii, Aphis craccivora and Rhopalosiphum maidi [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=3&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEAQFjAC&amp;amp;url=http%3A%2F%2Fwww.ets-staffing.com%2Fcurrentbiotica%2Fjournals6-issueiii%2Fcb-6-3-short-notes-3.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNETNWfEOrZznoJjj0ZrFFOINJ2liw], blackflies, psylla and leaf miner. Green lacewings are recommended for the integrated pest management programme (Nehare et al., 2004). M. boninensis feeds well on Corcyra cephalonica eggs[http://cabdirect.org/abstracts/20113350526.html;jsessionid=69EA3BE15508EF9C029CE688C342FD4D] (laboratory host) C. cephalonica can be mass reared on Jowar along with groundnut, streptomycin, vitamin complex, Na and K salts.&lt;br /&gt;
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[u]Control[/u]: &lt;br /&gt;
Spray Imidacloprid (0.005%; 0.3 ml/L water), acephate 75SP (1.5 g/L water), Etofenprox (0.03%), Phosalone (1.5 ml/L water), Carbaryl (0.15%; 3 g/L water), Monocrotophos (0.04%), Phosphamidon (0.05%) or Methyl Parathion (0.05%). &lt;br /&gt;
* Spray 3 times; First spray should be given during the early stage of flower formation. The second spray should be given at a flower size of 6 to 8 cm, still before blooming. The third spray should be given after fruit setting, when the fruits are the size of a pea. Or:&lt;br /&gt;
* First spray should be given during the early stage of flower formation. The second spray should be given 2 weeks later. Or:&lt;br /&gt;
* Spray 3 times; First spray of imidacloprid (0.005%; 0.3 ml/L water) should be given during the early stage of flower formation. The second spray, thiamethoxam (0.005%; 0.2 g./L water) or acephate 75SP (1.5 g/L water) should be given at after fruit setting. If hopper infestation persists, the third spray, of carbaryl (0.15%; 3 g/L water) should be given prior to fruit maturation.&lt;br /&gt;
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Imidacloprid residues persist in peel for 60 days and in pulp for 50 days. Mature Dashehari fruits at harvest (after 85 days of spraying) were free from imidacloprid residues.[http://www.ncbi.nlm.nih.gov/pubmed/23196371]&lt;br /&gt;
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==Mango Midges==&lt;br /&gt;
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[u]Inflorescence Midge[/u]&lt;br /&gt;
[[Image:Platygaster_sp.jpg|thumb|right| Platygaster sp. [http://liuzhen.000space.com/insect/organism.php?id=1367&amp;amp;type=list]]]&lt;br /&gt;
[[Image:Inflorescence_midge.jpg|thumb|left| [http://www.bitterrootrestoration.com/mango/inflorescence-midge-erosomyia-indica.html Erosomyia indica ©]]]&lt;br /&gt;
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This midge (Erosomyia indica) lays eggs in new inflorescence (preventing flower and fruit set) and new leaves around it, and then the larvae will eat their way through. The maggots penetrate and feed on the tender parts of the plant, such as shoots, buds and flower buds. The flowers will dry and fall off. For pupation, the mature larvae will drop into the soil. The adult midge lives only one day and doesn&amp;#039;t cause any damage. Maggots from eggs laid on new fruits will eat their way into the fruit, which will turn yellow and drop.[http://www.actahort.org/books/231/231_15.htm] The inflorescence midge is an important pest in India in particular.&lt;br /&gt;
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Infloresence midge is parasitized by Platygaster sp., Eupelmus sp. and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19881115612.html;jsessionid=E4CDBFFBEE75C82EDA1BA2CA7B5F1DD2], which all also parasitize Gall Midges. The inflorescence midge is also parasitized by Aprostocetus sp.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9] and Mirufens longifunculata. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false]&lt;br /&gt;
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Another mango midge, Procystiphora mangiferae is parasitized by Pirene sp. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], which also parasitizes mango gall midges.&lt;br /&gt;
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Chemicals: If you have no other option, Parathion, Dimethoate, Phosphamidon and Endosulfan much reduced infestations on mango shoots.[http://www.cabdirect.org/abstracts/19740517480.html;jsessionid=59B14F930CB8EDFD2F1B50F1B04C7692] The most effective insecticide for control appeared to be phosphamidon (Dimecron), whether mixed with diazinon or not, as a foliar spray. [http://www.cabi.org/isc/abstract/19770549886]&lt;br /&gt;
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[u]Gall midges[/u]&lt;br /&gt;
[[Image:Mango_galls.jpg|thumb|left| [http://www.infonet-biovision.org/default/ct/124/crops © A.M. Varela]]]&lt;br /&gt;
[[Image:Eupelmus_urozonus.jpg|thumb|right| Eupelmus urozonus [http://www.biolib.cz/en/image/id150459/ © Miroslav Fiala]]]&lt;br /&gt;
[[Image:Mango_galls_P_matteiana.jpg|thumb|left| P. matteiana galls [http://www.pests.blogfa.com/8907.aspx © RJ Gagné]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Gall_midget_exit_holes.jpg|thumb|left| Exit holes [http://biostor.org/reference/55262 © RJ Gagné]]]&lt;br /&gt;
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There are various mango gall flies:&lt;br /&gt;
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- Erosomyia mangifereae or Procontarinia frugivora has been observed in the Philippines, India, West Indies and Brazil.&lt;br /&gt;
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- Asynapta sp. in West Indies.&lt;br /&gt;
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- Dasyneura mangifereae in Hawaii.&lt;br /&gt;
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- P. allahabadensis, P. biharana, P. brunneigallicola, P. viridigallicola, P. echinogalliperda, P. keshopurensis, P. mangifoliae, P. tenuispatha, P. amraeomyia and Dasyneura amaramanjarae have been observed in India (and some in Pakistan).&lt;br /&gt;
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- P. mangicola in China and Japan.&lt;br /&gt;
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- P. matteiana (1.5 mm long) in India, Kenya, Mauritius, Java and Réunion[http://biostor.org/reference/55262][http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=4&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFEQFjAD&amp;amp;url=http%3A%2F%2Fwww.geocities.ws%2Fmdce2005%2FMDCE2005%2F27-98_102.pdf&amp;amp;ei=HS5zU-P3DomXyQOd_IHgCA&amp;amp;usg=AFQjCNHlZtUS8qYFE2lCVSqh3ow0K4ukLg&amp;amp;sig2=vW-1UFdjzeE3l58kmc8eMg&amp;amp;bvm=bv.66699033,d.bGQ]. &lt;br /&gt;
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The tiny (1-2 mm) mango gall flies lay their eggs on the surface of young leaves, buds, shoots and flowers. After hatching, the maggots will eat their way into the plant tissue. This will show as galls (3-4mm) on the leaves. Once mature, they drop to the ground to pupate. The holes that they left behind are susceptible to fungal infections. Infested fruits initially show small (1mm) brownish lesions, which grow as the fruit grows. In young fruits, the exit holes are usually near the point of attachment, on the bottom side. Most infested fruits fall to the ground before ripening. Optimal conditions for reproduction is 26°C and a relative humidity of 70 to 80%. Infestation particularly occurs at bud-burst stage, at fruit set and on tender leaves of new flushes.[http://openagricola.nal.usda.gov/Record/IND92003672]&lt;br /&gt;
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[u]Natural enemies[/u]; Parasitic insects such as Platygaster sp., Eupelmus sp., Tetrastichus sp., Systasis dasyneurae [http://www.cabdirect.org/abstracts/19881115612.html], Pirene sp. and the predator ants Formica rufa (aka red wood ant, native to Europe and North America), Oecophylla longinoda (weaver ant native to West and Central Africa) and Oecophylla smaragdina (native to South-east Asia) and Camponotus sp. (aka carpenter ant, native to forests world wide).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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[u]Baits[/u]; Coriander oil attracts Erosomyia. D. amaramanjarae is attracted by a mixture of glycine, sodium hydroxide, ammonium carbonate and strong ammonia solution. Bordeaux mixture is a repellent. &lt;br /&gt;
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[u]Chemicals[/u]; Various systemic insecticides are equally effective: Dimecron (phosphamidon), Anthio (formothion) and Metasystox (demeton-S-methyl). Most effective is a mixture of phosphamidon (0.03%) and diazinon (0.03%).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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==Mango Moths==&lt;br /&gt;
[[Image:Indarbela_quadrinotata.jpg|thumb|left| Indarbela quadrinotata [http://www.agriplaza.in/orange-protection.htm#indarbela © Agriplaza]]]&lt;br /&gt;
[[Image:Podagrionella_habitus.jpg|thumb|right| Podagrionella [http://www.waspweb.org/Chalcidoidea/Torymidae/Toryminae/Podagrionini/Podagrionella/index.htm © S. van Noort]]]&lt;br /&gt;
[[Image:Indarbela_quadrinotata_2.jpg|thumb|left| Indarbela quadrinotata [http://www.ku.ac.th/e-magazine/september43/bark_feeders/ © KUAC]]]&lt;br /&gt;
[[Image:Eudocima_phalonia.jpg|thumb|left| Eudocima fullonia 3&amp;lt;sub&amp;gt;rd&amp;lt;/sub&amp;gt; instar [http://www.ccs-hk.org/DM/butterfly/Noctuid/Eudocima-phalonia.html © Light Creations]]]&lt;br /&gt;
[[Image:Mantis_religiosa_5.jpg|thumb|right| Mantid eating large moth [http://www.agefotostock.com/en/Stock-Images/Rights-Managed/K60-589612 © Oriol Cabrero]]]&lt;br /&gt;
[[Image:Eudocima_fullonia_2.jpg|thumb|left| Eudocima fullonia [https://www.flickr.com/photos/bettaman/5191646324/ © Shipher Wu]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Eudocima_fullonia_cocoon.jpg|thumb|left| Eudocima fullonia cocoon [http://www.ccs-hk.org/DM/butterfly/Noctuid/Eudocima-phalonia.html © Light Creations]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Eudocima_homaena.jpg|thumb|left| Eudocima homaena [https://www.flickr.com/photos/suedechen/8063437610/ © Suede Chen]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-l5.php © PathPiva]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella_2.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-1.php © PathPiva]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Orthaga_exvinacea.jpg|thumb|left| Orthaga exvinacea [http://www.nbaii.res.in/insectpests/images/Orthaga-exvinacea7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Orthaga_euadrusalis.jpg|thumb|left| Orthaga euadrusalis [http://www.jpmoth.org/~dmoth/62_Pyralidae/6003_Epipaschiinae/60031001_Orthaga_euadrusalis_1865/Orthaga%20eudrusalis%200208287801.jpg]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_2.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42845]]]&lt;br /&gt;
[[Image:Aleiodes_indiscretus.jpg|thumb|right| Aleiodes indiscretus [http://commons.wikimedia.org/wiki/File:Aleiodes_indiscretus_wasp_parasitizing_gypsy_moth_caterpillar.jpg © Agricultural Research Service]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42848]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_3.jpg|thumb|left| Citripestis eutraphera [http://www.boldsystems.org/index.php/Taxbrowser_Taxonpage?taxid=375866 © ANIC/BIO]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_2.jpg|thumb|left| P. jocosatrix [http://www.nbaii.res.in/insectpests/images/Penicillaria-jocosatrix7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_3.jpg|thumb|left| P. jocosatrix [http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar © DAFF]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix.jpg|thumb|left| P. jocosatrix [http://www.flickr.com/photos/bettaman/3973171117/ ©]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Caterpillar.jpg|thumb|left| Deanolis sublimbalis [http://www.padil.gov.au/pests-and-diseases/pest/main/136263/43328 © S. Eyres]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Moth.jpg|thumb|left| Deanolis sublimbalis [http://www.ces.csiro.au/aicn/name_s/b_1295.htm © DAFF]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Chlumetia_transversa_2.jpg|thumb|left| C. transversa [http://www.nbaii.res.in/insectpests/Chlumetia-transversa.php © NBAII]]]&lt;br /&gt;
[[Image:Chlumetia_transversa.jpg|thumb|left| C. transversa [http://www.inaturalist.org/observations/572035 © T Bonebrake]]]&lt;br /&gt;
[[Image:Goryphus_basilaris.jpg|thumb|right| Goryphus basilaris [http://www.hkwildlife.net/viewthread.php?tid=49119 © Daydream]]]&lt;br /&gt;
[[Image:Parasa_lepida.jpg|thumb|left| P. lepida [http://fanseab.exblog.jp/9707580]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Parasa_lepida_2.jpg|thumb|left| P. lepida [http://www.isan.clubs.chula.ac.th/para_norkhai/?transaction=post_view.php&amp;amp;cat_main=all&amp;amp;id_main=301&amp;amp;star=270&amp;amp;pg=28 © ปิ่นลม]]]&lt;br /&gt;
[[Image:Parus_major.jpg|thumb|right| Parus major [http://en.wikipedia.org/wiki/Parus_major#mediaviewer/File:Parus_major_2_Luc_Viatour.jpg © Luc Viatour / www.Lucnix.be]]]&lt;br /&gt;
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[u]Bark Eating Caterpillar[/u]&lt;br /&gt;
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The Bark Eating Caterpillar (Indarbela quadrinotata and Indarbela tetraonis) aka Mango Bark Feeder has been observed in India, Bangladesh and Sri Lanka. The moth has a wingspan of maximally 2 cm. This wood boring insect may attack a wide variety of trees, including mango, guava, jujube, pomegranate and apple trees. In plantations, infestation generally starts with the onset of premonsoon rains. The main symptom is plastered dark-brown masses on tree trunks and large branches. These webbed masses consist of chewed on wood remainders / frass, excrements and silken thread. Eggs are laid in clusters on the bark of trees. Initially, the newly hatched larvae occur in groups browsing on the bark. Then they migrate and lodge in shoots and buds. As the larvae grow, they start tunneling into the wood, up to 25 cm deep. This tunnel is kept free of frass. The frass and excreta from the tunnels are used for constructing the extended tunnel mouth (sleeve). As the larvae get bigger, they make the tunnel wider. The larvae hide in these tunnels during the day and come out at night to eat the surrounding bark of the tree. The rate of feeding is faster during the hottest months. The larva minimally eats 16 mg / day, at 12.5 degrees Celsius. The maximum is 166 mg/day at 35 degrees Celsius. Similarly, food consumption is maximum at 96% RH and minimum at 56% RH.&lt;br /&gt;
When large patches of bark are eaten away, the tree gets increasingly exposed to various bacteria, fungi etc. And if substantial areas of bark have been eaten, the tree will eventually die. Prolonged water stress decreases the resistance of the host tree. The larvae pupate inside the tunnel and the emerging moth leaves its &amp;quot;pupal skin&amp;quot; at the mouth of the borer hole. The pupa is 1.5 cm long. The larval stage lasts for about 8 months. The pupal period lasts for about 9 days. It has a little more than one generation per year.  [http://www.tropecol.com/pdf/open/PDF_49_1/09%20Shashidharan.pdf]&lt;br /&gt;
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Natural enemies; Two fungi; Aspergillus candidus and Beauveria bassiana have been reported to cause mortality of the Bark Eating Caterpillar in the field. Laboratory trials have indicated 100% larval mortality by these fungi. The Bark Eating Caterpillar is parasitized by Podagrionella indarbelae (&amp;#039;metallic&amp;#039; parasitic wasps), which may be fed the eggs of I. tetraonis.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf] Podagrionella is found in tropical African countries, Senegal, Algeria, Malawi, France, Spain, India, Thailand, Indonesia, The Philippines, Australia&lt;br /&gt;
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Control; prevent overcrowding by sufficient spacing in between trees. One may insert a sharp metallic probe/spike into each tunnel/hole to kill the larvae. One may seal the tunnel entrance using tar or wax. Of the various insecticides screened against this insect, monocrotophos (0.1 %), quinalphos (0.1 %) and fenvalerate (0.08%) gave best results.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf]&lt;br /&gt;
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[u]Fruit-piercing Moths[/u] &lt;br /&gt;
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Eudocima (aka Othreis) are very large moths (up to 10 cm wingspan) with orange abdomen. The most widespread specie is Eudocima fullonia (aka Eudocima phalonia)[http://www.hindawi.com/journals/tswj/2011/753484/], one of the most damaging in many tropical lowland regions from West Africa eastwards to the Pacific [http://www.thaiscience.info/journals/Article/Biological%20and%20taxonomic%20studies%20on%20immature%20and%20adult%20fruit-piercing%20moths%20in%20nepal,%20with%20reference.pdf], also predominant in India.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41] Eudocima moths look like dead leaves when in hiding mode. Until they show their bright orange hindwings with broad black margins and a large black spot in the middle. Mature larvae are 4 to 5 cm long. These moths have been observed in virtually any tropical country, except for the Americas. The adults fly (very well), feed and mate during the night. In tropical countries, they live up to a month. The adults feed on fruit juice by puncturing the fruit. The resulting wound is used by various bugs and diseases to attack the fruit (including mango, banana, grapes, orange, clementine, kiwi, lychee, pineapple, peach, apricot, papaya apple and pear). Eggs are laid singly, or in masses (up to 750 in a cluster, which may be used for rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings]), on any part of the plant. In tropical conditions, eggs hatch within 4 days. Larvae may feed around the clock and mature in 3 weeks, after which they pupate in cocoons of silk-spun leaves. Pupation favors wet conditions and occurs in 12 to 18 days.[http://www.aphis.usda.gov/plant_health/plant_pest_info/pest_detection/downloads/pra/efulloniapra.pdf] Pupation is always in a roomy cell made of living leaves woven together with silk and slightly lined; the pupa is attached to this lining by the cremaster.[http://www.mothsofborneo.com/part-15-16/calpini/calpini_4.php] Temperatures below 16°C during the activity period after dusk prevents feeding, mating and oviposition.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] Eudocima fullonia was most destructive in Nigeria and Sierra Leone.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41]&lt;br /&gt;
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Their larvae do not feed on mangoes, nor the mango trees. Instead, the larvae feed on very specific Fabaceae and Menispermaceae. Variation in the alkaloids associated with certain menisperm genera (eg quaternary alkaloids in Tinospora[https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2007-969933]) may explain the very specific (or even exclusive) moth–host plant relationships.[http://www.publish.csiro.au/?paper=SB9960227] Eudocima larvae are known to feed extensively on Stephania japonica (Menispermaceae) and its varieties.[http://www.calodema.com/freefiles/412.pdf] In Australia the larvae of Eudocima salaminia feed almost exclusively on the forest vine, Stephania japonica.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] Eudocima materna feeds exclusively on Tinospora cordifolia [https://books.google.nl/books?id=UxVFMwEACAAJ&amp;amp;dq=inauthor:%22BHUMANNAVAR+BASAVARAJ+S%22&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=MHaIVL2mBMLwUvDcg5gF&amp;amp;ved=0CCIQ6AEwAA] (widespread in tropical regions [http://link.springer.com/article/10.1007/s00606-004-0293-1#page-1], including Africa[http://informahealthcare.com/doi/abs/10.1080/14756360802333075]).Eudocima homaena may feed on Quisqualis indica.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41], Achyranthes, Cocculus, Cyclea, Menispermum and Tiliacora species.[http://www.en.inforapid.org/index/index.php?search=Cocculus] Eudocima jordani and Eudocima fullonia may be generalist feeders.[http://www.researchgate.net/publication/249439696_Differential_habitat_affinities_of_five_species_of_fruitpiercing_moths_(Lepidoptera_Noctuidae)_in_their_utilization_of_Tinospora_smilacina_Benth._as_a_larval_host_plant_in_north_Queensland]&lt;br /&gt;
Eudocima fullonia larva feed on Erythrina variegata (Indian coral tree in Eastern Africa) and Stephania japonica [http://www.nhm.ac.uk/resources/research-curation/projects/chalcidoids/pdf_x/sandsli2005.pdf], but growth is better and mortality much lower when feeding on Sarcopetalum harveyanum and particularly Tinospora smilacina (Menispermaceae - the moonseed family), in comparison to Stephania japonica. Tiliacora are also readily accepted by Eudocima fullonia larva.[http://onlinelibrary.wiley.com/doi/10.1111/j.1570-7458.1994.tb01803.x/abstract] Eudocima fullonia larva feed on leaves of Erythrina and vines in the Menispermaceae [http://www.cabdirect.org/abstracts/20066600377.html], including Tinospora homosepala [http://www.wptrc.org/userfiles/file/Physiol%20Entomol-2005.pdf], Tinospora sinensis [http://www.ccs-hk.org/DM/butterfly/Noctuid/Eudocima-phalonia.html], Tinospora cordifolia (in India, Myanmar and Sri Lanka) [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=23012], Tinospora baenzigeri, Tinospora crispa, Tiliacora triandra  (in Thailand).[http://www.thaiscience.info/journals/Article/Biological%20and%20taxonomic%20studies%20on%20immature%20and%20adult%20fruit-piercing%20moths%20in%20nepal,%20with%20reference.pdf] Cocculus hirsutus [http://www.actahort.org/members/showpdf?booknrarnr=890_81], Carronia multisepala, Hypserpa decumbens (in Australia) [http://onlinelibrary.wiley.com/doi/10.1111/j.1570-7458.1994.tb01803.x/abstract] and Legnephora moorei (Australia) [http://onlinelibrary.wiley.com/doi/10.1111/j.1442-9993.1993.tb00471.x/abstract]&lt;br /&gt;
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Tinospora smilacina (aka Snake vine, Tinospora walcottii, Tinospora berneyi) is a tropical herbaceous vine endemic to Australia [http://keys.trin.org.au/key-server/data/0e0f0504-0103-430d-8004-060d07080d04/media/Html/taxon/Tinospora_smilacina.htm], found along the boundaries of dry rainforest and dry eucalyptus forest.[http://www.somemagneticislandplants.com.au/index.php/plants/600-tinospora-smilacina] Tinospora fragosa is present in south Africa.[http://www.sciencedirect.com/science/article/pii/S0254629910001158] In Ghana, the following species occur: Tiliacora leonensis [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=120], Tiliacora funifera [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=80], Tiliacora dinklagei [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=140], Tiliacora louisii [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=240] and Tiliacora dielsiana [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=260] &lt;br /&gt;
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Control; Regularly check the orchard during the night using a flashlight. The adult Eudocima moths have large eyes that glow red when illuminated. Adult [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] may eat large moths such as Fruit-piercing moths. The female Praying mantis lays a few hundred eggs in a cluster (ootheca) attached to an object like a branch or trunk. Large species of mantids may have a length of up to 12 cm. Their colour is usually adapted to the environment they naturally live in. They have about 2 generations per year. Young mantids look like adults, but don&amp;#039;t have wings. Adult females often have no wings either, or reduced wings.&lt;br /&gt;
Eudocima fullonia is parasitized by Euplectrus maternus [http://link.springer.com/article/10.1023/B:BICO.0000036439.74117.2f#page-1], Euplectrus melanocephalus [http://www.aapmhe.in/index.php/pmhe/article/view/42] and Trichogramma.[http://images.peabody.yale.edu/lepsoc/jls/2010s/2011/2011-65-1-053.pdf]&lt;br /&gt;
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[u]Honeydew Moth[/u]&lt;br /&gt;
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The Honeydew Moth (Cryptoblabes gnidiella aka Albinia casazzar aka Albinia wockiana) aka Christmasberry Moth survives only in warm climates (all over the world). It attacks a large variety of crops, including mangoes, banana, oranges, clementines, grapes, loquats, pomegranates, avocado, coffee, maize and rice. The caterpillars (1 cm long) mainly feed superficially on fruits, but may also feed on leaves, flower, shoots and bark. The newly hatched larvae and the adults, however, solely feed on honeydew. The adults are active during the night. Females mate 1 to 4 times during their entire life. One Honeydew Moth may lay up to 100 eggs within 2 days after mating, on fruit or leaves (averagely 150 in total, during lifetime). These eggs hatch within one week (or longer, in colder regions). It has several generations per year, depending on the climate. Adult moths are good flyers (1 to 2 cm wingspan) and may rapidly spread to poorly maintained orchards (infested with honeydew).&lt;br /&gt;
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Natural enemies; Scolothrips sexmaculatus and Orius spp. prey on the eggs and early-instar larvae. The Honeydew Moth is parasitized by Phanerotoma sp.[http://www.cabdirect.org/abstracts/19740514736.html] and Trichogramma Platneri (both parasitoid wasps).&lt;br /&gt;
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Trichogramma are extremely tiny wasps (&amp;lt; 0.5 mm). They insert their eggs into the eggs of the moth. The larvae feed, grow and pupate inside the host egg, which converts the colour of the host egg from white to black. The emerging wasp eats its way out and is ready to mate. The eggs from unmated females will produce only males, whereas the eggs from mated females will produce both males and females. Adult Trichogramma feed on nectar from flowers. One female wasp may parasitize 50 moth eggs during her life. The optimimum conditions for Trichogramma are 20 to 27 degrees Celsius and 60% RH. Most insecticides kill Trichogramma.&lt;br /&gt;
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Control; This moth is attracted by honeydew secreted by aphids, scale insects and other honeydew secreting bugs. It is therefore most effectively controlled by controlling all honeydew secreting bugs. Its instars are highly susceptible to Bacillus thuringiensis.&lt;br /&gt;
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[u]Leaf Webber[/u]&lt;br /&gt;
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These brown moths (Orthaga euadrusalis and Orthaga exvinacea) are a minor mango (and Cashew nut) pest. They have been observed in India and Indonesia. Females mate only one time during their whole life.[http://www.ncbi.nlm.nih.gov/pubmed/20136014] The leaf webber lays eggs on young leaves and buds and creates a web around pods, leaves and flowers for its larvae to pupate in. Such a webbed cluster may contain several larvae. The Leaf Webber causes relatively very little damage, as the larvae merely scrape and eat from the surface of the leaves.&lt;br /&gt;
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Control: Application of Serratia marcescens (a bacterium), Aspergillus flavus (a fungus) and Beauveria bassiana (a fungus) are all very effective.[http://www.cabdirect.org/abstracts/19810586732.html] The leaf webber is parasitized by Brachymeria lasus, Hormius sp., Pediobius bruchicida and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html] and also by Trichogramma chilonis and Trichogramma pretiosum.[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=37933]&lt;br /&gt;
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[u]Mango Fruit Borer[/u];&lt;br /&gt;
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The Mango fruit borer (Citripestis eutraphera; a moth) has been observed in Indonesia, India and Australia. Its lays its eggs (&amp;lt; 1 mm) on fruit or stalk. The larvae feed on mango pulp (and cashews) and cause premature fruit drop, mainly in young fruit. The larvae hatch in 2 days. They are initially white, then red-violet when young, turning to dark blue as they grow. Hatched larvae initially feed on the peel of the fruit. Then they bore into into the fruit and feed for 12 to 15 days before they pupate in the fallen fruit, or in the soil. Often there is more than one larva in a fruit with a total of 15 recorded in one fruit.[http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42846].&lt;br /&gt;
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Control: Its natural enemies are parasitoid wasps (Aleiodes sp. and Euplectrus sp.) and a parasitoid fly (Blepharella lateralis).&lt;br /&gt;
[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=9416] To protect the stems, cover them with a cloth or Jute and paste charcoal over it. Fostoxin tablets can also be placed and sealed in the holes made by the borers.&lt;br /&gt;
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[u]Mango Shoot Borer[/u]&lt;br /&gt;
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Mango Shoot Borer (Penicillaria jocosatrix) or Velvet mango slug; P. jocosatrix (wingspan 2 - 2.5 cm) has been observed in Indonesia, The Philippines, Malaysia, Vietnam, Guam, Hawaii, Thailand and Australia. Its larvae; the caterpillar of this moth (up to 3 cm long) causes damage to shoots, stems, inflorescence, flowers and fruits of the mango tree. Pestalotiopsis anacardiacearum sp. nov. (fungal leaf rot) is found on dead mango leaves associated with P. jocosatrix (the Mango Shoot Borer).[http://biotaxa.org/Phytotaxa/article/view/phytotaxa.99.2.1] Eggs hatch in 3-5 days. Larval development takes 8-10 days. Mature larvae pupate in the soil and the moth emerges 16-20 days later.[http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar]&lt;br /&gt;
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Natural enemies: Euplectrus sp. (a parasitoid wasp) and Blepharella lateralis (a parasitoid fly) may effectively decrease Mango shoot borer infestation [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], and are also effective against the Mango fruit borer.&lt;br /&gt;
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[u]Mango Seed Borer[/u]&lt;br /&gt;
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The Mango Seed Borer (Deanolis sublimbalis aka Autocharis albizonalis) is the Red banded mango caterpillar. It is a mango pest in Australia, Burma, The Philippines, Malaysia, Vietnam, China, Indonesia and Papua New Guinea. Severe infestation may cause up to 50% yield reduction. This grey snout moth (wingspan 2 cm) lays its eggs on the top of the mango. Its larvae (one or more per fruit) develop within the fruit in 2 to 3 weeks. Initially they feed on the pulp, and then on the seed. The fruit will split, rot and drop. The mature larvae will pupate in the soil.&lt;br /&gt;
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Natural enemies: Rychium attrisimum are wasps that feed on the larvae of the Mano Seed Borer as they leave the fruit.[http://www.planthealthaustralia.com.au/wp-content/uploads/2013/03/Red-banded-mango-caterpillar-CP.pdf] Trichogramma chilonis and Trichogramma chilotreae are tiny parasitoid wasps that parasitize the eggs of the Mango Seed Borer and hundreds of other moths. They are so small that with the naked eye you cannot see what they are. They have a wingspan of 0.5 mm. They live about 2 weeks, and may lay several eggs in a single moth egg.&lt;br /&gt;
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Chemicals: If you have no other option, Deltamethrin and Cyfluthrin are most effective.&lt;br /&gt;
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[u]Mango Tip Borer[/u]&lt;br /&gt;
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Mango Tip / Shoot / Bark / Trunk / Twig Borer (Chlumetia transversa) aka aka Shoot Boring Caterpillar is a serious mango (and litchi) pest. The major symptom is drying of the tip of young shoots. Female moths lay their eggs on tender leaves. Newly hatched larvae bore into these leaves. As they grow they bore into young shoots near the growing point, and subsequently tunnel down. The leaves of affected shoots will droop. It has four generations each year, and in colder regions overwinters as pupae in young branches and bark. Generations are overlapped.[http://europepmc.org/abstract/CBA/367089] Full grown caterpillars are 2 to 2.5 cm long.&lt;br /&gt;
C. transversa has been observed in Indonesia, The Philippines, Malaysia, Thailand, Taiwan, China, India, Pakistan, Sri Lanka and Australia. &lt;br /&gt;
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The Mango Tip Borer is parasitised by Bracon greeni, Meteorus sp. and Goryphus sp [http://www.cabdirect.org/abstracts/19810586735.html], which are all parasitoid wasps. Its larvae are also parasitized by the fly Megaselia chlumetiae sp. nov. Its larvae enter the host and develop by consuming the host&amp;#039;s internal tissues. Pupariation normally took place within the host&amp;#039;s empty skin.[http://www.cabdirect.org/abstracts/19921163681.html]&lt;br /&gt;
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Control; If you have no other option, spraying 2.5% deltamethrin EC for three times may be most effective (87% control efficiency) and the least harmful to the tree. Four insecticides tested; 80% dichlorvos EC(dilution of 1250), 47.5% chlorpyrifos EC(dilution of 1000), 2.5% deltamethrin EC (dilution of 2500) and 2% abamectin EC(dilution of 2000) were applied on mango trees during 2 months. Second best was 2% abamectin EC at a dilution of 2000. The other two insecticides were less effective, which might be due to the development of resistance in pests against these insecticides on account of longer repeated applications.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GXNY200910010.htm]&lt;br /&gt;
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[u]Nettle Caterpillar[/u]&lt;br /&gt;
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The Nettle Caterpillar, aka the Blue-striped Nettle Grub (Parasa lepida aka Latoia lepida; 2 to 3 cm long) has been observed in China, Pakistan, India, Sri Lanka, Bangladesh, Vietnam, Thailand, Malaysia, Japan and especially Indonesia. Parasa lepida may infest various fruit trees, including mango, cherry, persimmon, Indian almond and rose apple, and also coffee, tea, cocoa and palms, with a preference for coconut trees. The caterpillar of this species have spines containing a poison that causes serious irritation and inflammation in humans upon contact. It feeds on the leaves and shoots of the mango tree. Initially, defoliation will be localized, affecting only one or a few trees. Thus the infestation is easily identified. Subsequently, as new generations of this pest emerge, the infested area may rapidly expand. The female moth lays eggs on mango leaves. The cocoons are laid side by side. The cocoons are so hard and stout that they remain on the trunks for 5 to 6 years after spinning. Cocoons from which adults have successfully emerged have clear emergence holes. Newly hatched caterpillars will feed on the underside of the epidermis, stripping it off the leaflets. They often begin where the eggs were laid; at the tip. Then they eat the edges of the leaflet and eat large areas of the lamina. When they have finished developing, the whole leaflet will have been consumed systematically from tip to base, leaving only the midrib. On this midrib, the notched indentations are the only visible remainders left by the caterpillars. [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=38935]&lt;br /&gt;
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Parasa lepida is predated on by Agriosphodrus dohrni (The Assassin Bug, native to China, India, Indonesia). This 2 cm long Assasin Bug may be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
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The great tit (Parus major) is a predator of Parasa lepida cocoons in Japan. The most effective parasites are Apanteles parasae (a parasitoid wasp) and Chaetexorista javana (a parasitoid fly).[http://www.cabdirect.org/abstracts/19820596771.html]&lt;br /&gt;
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Salt spray from the sea and the resulting plant stress (considerable damage) may negatively affect the Parasa lepida moth.[http://www.bioone.org/doi/pdf/10.3956/0031-0603-83.3.193]&lt;br /&gt;
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==Mango Scales==&lt;br /&gt;
[[Image:Coccus_viridis.jpg|thumb|left| Coccus viridis [http://www.insectimages.org/browse/detail.cfm?imgnum=5385208 © J.W. Lotz]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| M. signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
[[Image:Coccus_hesperidum.jpg|thumb|left| Coccus hesperidum [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/09Cocc_hesperidum/4hesperidumHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Ceroplastes_floridensis.jpg|thumb|left| Ceroplastes floridensis [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/03Cero_floridensis/4floridensisHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Chilocorus_kuwanae.jpg|thumb|right| C. kuwanae [http://animal.memozee.com/view.php?tid=2&amp;amp;did=10472 © Jinsuk Kim]]]&lt;br /&gt;
[[Image:Aulacaspis_tubercularis.jpg|thumb|left| Aulacaspis tubercularis[http://gaga.biodiv.tw/new23/9409/076.htm]]]&lt;br /&gt;
[[Image:Azya_orbigera.jpg|thumb|right| A. orbigera [http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php © E Arcaya]]]&lt;br /&gt;
[[Image:Drosicha_mangiferae.jpg|thumb|left| D. mangiferae [http://www.jugantor.com/last-page/2014/04/19/89802 ©]]]&lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri.jpg|thumb|right| C. montrouzieri[http://www.ozanimals.com/Insect/Mealybug-Ladybird/Cryptolaemus/montrouzieri.html ©]]]&lt;br /&gt;
[[Image:Rastrococcus_iceryoides.jpg|thumb|left| R. iceryoides [http://www.nbaii.res.in/insectpests/Rastrococcus-iceryoides.php © NBAII]]] &lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri_larva.jpg|thumb|right| C. montrouzieri larva [http://www.insectimages.org/ © J.W. Lotz, FDACS Bugwood.org]]]&lt;br /&gt;
[[Image:Paracoccus_marginatus.jpg|thumb|left| Papaya mealybug [http://www.ablturismo.com/avispa-enviada-por-correo-salva-cultivos-en-la-india/ ©]]]&lt;br /&gt;
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The main mango scales are:&lt;br /&gt;
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* Coccids ; Coccus viridus (soft green scales) and Coccus hesperidum (soft brown scales)&lt;br /&gt;
* wax scales ; Ceroplastes spp.&lt;br /&gt;
* armoured scales ; Aulacaspis tubercularis, Chloropulvinaria polygonata (the Mango Green Shield Scale) and Aspidiotus destructor.&lt;br /&gt;
* Mealybugs (pseudococcidae) ; Drosicha mangiferae, Rastrococcus iceryoides (&amp;quot;Mango mealybugs&amp;quot;) and Paracoccus marginatus (&amp;quot;Papaya mealybug&amp;quot;, which is also a mango pest)&lt;br /&gt;
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Mango scales suck the sap from the leaves, and as a result, the leaves start drying. This may cause branches to die, blemished fruits and premature dropping. Scales are small insects (1 to 7 mm), generally immobile; as if shells are glued to the plant. Only the newly hatched crawlers (emerging from under a big scale) move to their feeding site. Soft scales produce honeydew, which may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould]. Armoured scales don&amp;#039;t excrete honeydew. The papaya mealybug has been observed in Mexico, Florida, Antigua, Cuba, Dominican Republic, Guadeloupe, Haiti, St Kitts, Martinique, Puerto Rico, St Martin, St Barthélémy, Virgin Islands.[http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=6&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEIQFjAF&amp;amp;url=http%3A%2F%2Fwww.cabi.org%2FISC%2FFullTextPDF%2F2013%2F20133231104.pdf&amp;amp;ei=VeZ8U_eFNaqX1AXh_YHYDA&amp;amp;usg=AFQjCNGWK-GGi3IRhAfSNBg6nah3XhoKWw&amp;amp;bvm=bv.67229260,d.d2k]&lt;br /&gt;
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[u]Natural enemies[/u]; Natural enemies of scales include parasitic wasps, ladybird beetles and lacewings, which are killed by broad-spectrum pesticides. Because of their honeydew secretion, scales may be protected / farmed by specific ants (eg arboreal ants (Azteca instabilis) farming soft green scales).[http://www.ncbi.nlm.nih.gov/pubmed/18559179] Some fungal pathogens effect this symbiotic interaction.[http://www.ncbi.nlm.nih.gov/pubmed/23905728] Parasitoid insects use the honeydew secreted by coccids (and aphids etc) as an infochemical to locate their hosts.[http://www.ncbi.nlm.nih.gov/pubmed/15112724] R. iceryoides is parasitized by Dinocarsis sp., Microterys flavus, Metastenus concinnus and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
Of the parasitoids Acerophagus papayae and Anagyrus loecki, A. papayae is the most effective in decimating the papaya mealybug. (Meyerdirk)&lt;br /&gt;
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[u]Lacewings[/u]; Most lacewings (also) feed on plant material, such as pollen. Particularly green lacewings such as Mallada boninensis, Mallada astur and Mallada signata are mainly predatory and very effective. They are relatively small (length up to 1.5 cm, including wings). Adults are active during the night, and may be attracted by flashlight. Eggs are attached to leaves and hatch within 4 days. The larval period lasts 12 days, and the pupal period lasts about 9 days. Adults live just one month. One may commercially rear millions of green lacewings for biological control of mango pests.&lt;br /&gt;
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[u]Cryptolaemus montrouzieri[/u] (aka Mealybug Ladybird) is a (4 mm long) predatory ladybird that eats mealybugs, soft scales (Coccidae) and armoured scales (Diaspididae). Originally from Australia, introduced in Florida and commercially available as a mealybug control agent. It needs warm, humid weather. Survival temperature range is 15-35°C. Life cycle on average is 30 days under optimum conditions: 23 °C and 60-70% humidity. Optimal temperature for adults is 28°C. They are active between 16°C and 33°C, and 70 to 80% RH. Longevity of females is 70 days max. It produces 4 generations per year. Development is accelerated at higher temperatures. At 25°C it may lay a total of 200 to 700 eggs (av. 400), at the rate of averagely 9 eggs per day. They preferrably lay their eggs in the egg mass of mealybugs. The eggs hatch in 5 to 6 days. There are 4 larval stages, and the entire larval period lasts about 12 to 17 days. At pupation stage they are about 13 mm long. Adults emerge after 7 to 10 days. These beetles prefer high concentrations of mealybugs to predate on, and will fly away when concentrations of mealybugs are lower.[http://www.arabscientist.org/english/page/11/] During its larval development, one C. montrouzieri may eat 2400 eggs of C. polygonata.[http://www.cabdirect.org/abstracts/19981112241.html] Unfortunately, the filaments produced by its larvae look very similar to mealybugs. Initially release 1 or 2 new active beetles per square meter in the morning, before it gets hot. Subsequently regularly release 2 to 3 beetles per tree. The beetles will be killed by pesticides, but tolerate copper-fungicides.&lt;br /&gt;
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[u]Chilocorus kuwanae[/u]; This ladybird beetle is a primary predator of wax scales (Ceroplastes sp.). This ladybird beetle is attracted by (spraying) methyl jasmonate, mainly due to the terpenoid compound alpha-pinene [http://www.ncbi.nlm.nih.gov/pubmed/19825299]&lt;br /&gt;
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[u]Microweisea coccidivora[/u]; a tiny (1mm long) predatory ladybird beetle from Florida and South Carolina (USA) that feeds particularly on armoured scales.&lt;br /&gt;
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[u]Azya orbigera[/u]; The larvae of this ladybird beetle predate on Coccus viridis. These larvae are relatively immune to ant attacks (ants that farm coccids), due to their sticky waxy filaments. Also, the presence of ants reduces A. orbigera larvae getting parasitized.[http://www.ncbi.nlm.nih.gov/pubmed/18348805] A. orbigera has been observed in Colombia, Guyana, Venezuela, Bélize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, México, USA, Trinidad and the West Indies.[http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php]&lt;br /&gt;
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[u]Sumnius cardoni[/u] (a predatory ladybird) predates on nymphs of D. mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19810586735.html] &lt;br /&gt;
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[u]Coccodiplosis sp[/u] (a parasitic gall midge)[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9], Cybocephalus sp. (a predatory beetle) and Scymnus coccivora (a predatory ladybird) predate on R. iceryoides (mealybug). &lt;br /&gt;
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[u]Cryptochetum sp.[/u] (parasitic flies) parasitize the 1st- and 2nd-instar nymphs of the Drosicha mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
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[u]Coccophagus scutellaris[/u] parasitizes Coccus hesperidum by laying eggs in its gut.[http://www.ncbi.nlm.nih.gov/pubmed/747455] When parasited and parasiteless hosts are available, the female of Coccophagus deposits more eggs on the latter.[http://www.ncbi.nlm.nih.gov/pubmed/7283545]&lt;br /&gt;
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[u]Thomsonisca pakistanensis[/u] is a parasitic wasp that most commonly parasitizes Aspidiotus destructor. A. destructor is also parasitized by Aneristus ceroplastae (tiny parasytic wasp), Comperiella bifasciata (parasytic wasp also effective against yellow scales; Aonidiella citrina[http://www.publish.csiro.au/paper/ZO9710053.htm]), Chartocerus sp. and Chrysonotomyia sp. (both also parasytic wasps) [http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
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[u]Aphytis sp[/u]; This tiny (2 to 3 mm long) parasytic wasp parasitizes Aulacaspis tubercularis (up to 46% parasitism in a test).[http://www.actahort.org/books/509/509_96.htm]&lt;br /&gt;
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[u]Lecanicillium lecanii[/u]; This fungus kills various coccids, including Coccus hesperidum.[http://www.ncbi.nlm.nih.gov/pubmed/20863711] Also Ceroplastes sp. may be killed by Lecanicilliurn lecanii. Adding body materials of life coccids to a multi-generation culture (medium), significantly keeps the vigor and higher virulence of this fungus.[http://www.ncbi.nlm.nih.gov/pubmed/20387464]&lt;br /&gt;
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[u]Control[/u]: Collect the affected leaves and burn them. Spraying chlorogenic acid (a phenol naturally present in coffee beans) stimulates locomotory activity of the green scale Coccus viridis, thus minimizing their feeding. [http://www.ncbi.nlm.nih.gov/pubmed/20857759]&lt;br /&gt;
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If you have no other option: Use Metasystox 25% EC at the rate of 0.3 L in 450 L water or Fotidal 50 EC at the rate of 0.5 L in 450 L water / acre. Or spray mineral oil at low concentrations (not damaging the trees) after fruit picking, but not during flowering. Don&amp;#039;t spray during droughts or excessive heat.&lt;br /&gt;
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==Weevils==&lt;br /&gt;
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[u]The Mango Stone Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_mangiferae.jpg|thumb|left| Mango Stone Weevil [http://en.wikipedia.org/wiki/Sternochetus_mangiferae#mediaviewer/File:Mango_seed_weevil_2.jpg © P.Jeganathan]]]&lt;br /&gt;
[[Image:Oecophylla_smaragdina.jpg|thumb|right| Stone Weevil captured by O. smaragdina [http://www.cabi.org/isc/datasheet/16434 © Renkang Peng]]]&lt;br /&gt;
[[Image:Mantis_religiosa.jpg|thumb|right| Praying mantis [http://www.statesymbolsusa.org/Connecticut/insect_praying_mantis.html © Andy Williams / CritterZone]]]&lt;br /&gt;
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The Stone Weevil (Sternochetus/Curculio/Rhynchaenus/Cryptorhynchus mangiferae) aka Mango Seed / Nut Weevil is a short weevil; 7 mm to 1 cm long and 4 mm wide. It possesses a tough exoskeleton. Weevils are a type of beetle. The scales of the Stone Weevil may be of various colours; shades of black, grey, red or yellow. The Stone Weevil has been observed in many mango-producing countries all over the world (including Ghana, Nigeria, Kenya, Mozambique, Tanzania, Uganda, Zambia, India, Bangladesh, Indonesia, Thailand, Malaysia, Australia, USA)[http://www.cabi.org/isc/datasheet/16434]. Adult weevils can hardly fly, and this pest spreads by fruit transports. At dusk and during the night, the adults feed on leaves and young shoots. The female lays her eggs on and just under the surface of the peel of young (about 3 cm long) mangoes. Each female can lay up to 15 eggs per day and may lay several eggs on one fruit. The Stone Weevil prefers the sweetest (high-sugar) varieties of mango. After laying an egg, the weevil will make an incision in the fruit to let the sap from the fruit cover the egg, and to make it stick to the fruit. The amber-coloured sap will harden out, and remain as a protective resin mark over the egg. Newly emerged grubs are white and slender, and have no legs. They bore their way through the pulp towards the seed, within 2 days. Larvae and pupae develop inside the fruit. Pupation occurs in the seed. No external symptoms of attack are readily visible on infested fruits. Once matured (which takes up to 2 months), they eat their way out. About 25% of adult the weevils over-winter in the seed. They can live 2 years. One generation is produced each year.&lt;br /&gt;
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Natural enemies; The Stone Weevil is effectively predated on by Weaver ants such as Oecophylla smaragdina and Oecophylla longinoda.[http://iiste.org/Journals/index.php/JBAH/article/view/12284] Weaver ants, however, may also farm (for the produced honeydew) and protect aphids and various mango scales against their natural enemies. &lt;br /&gt;
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Weevils are also predated on by [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids], which may eat anything they can catch, including flies, beetles and moths. Adults readily mate in captivity and have one generation per season. Rearing mantids requires rearing of other insects, such as aphids and drosophilae (for nymphs), tephritids (for young mantids) and moths (for adult mantids) in large quantities. Females lay their eggs in a sticky cluster of several cm long. Nymphs (very tiny praying mantids) emerge from eggs. The developing nymphs need to be separated and fed aphids/drosophilae, or they will become cannibalistic.&lt;br /&gt;
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Control; Collect and destroy all fallen fruits, seeds, leaves, twigs etc. If you have no other option, spraying Fenthion (0.01%) on the trees at the time of oviposition is found to be effective. Fenthion, however, is extremely toxic to beneficial insects [http://www.actahort.org/books/509/509_96.htm], so that applying Fenthion may lead to secondary infestation of Mealybugs. A single spray of tiametoksam (Actara™ SC 240g/ℓ a.i.) at the roots during flower set may be effective for seasonal control.[http://etd.uovs.ac.za/ETD-db/theses/available/etd-09182009-083002/unrestricted/LouwCE.pdf] In Alphonso and Bagan Pali mangoes, a single spray (at dusk) of Monocrotophos 36EC 1ml per 1 litre of water was found to be very effective (97.5% to 100% mortality) [http://issuu.com/kisanadmin/docs/mango1/16] In a laboratory test, Beauveria bassiana was somewhat effective (30% mortality in 14 days), but in an orchard setting there was no effect at all.[http://www.cabdirect.org/abstracts/19971101160.html]&lt;br /&gt;
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[u]The Mango Pulp Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_frigidus.jpg|thumb|left| Mango Pulp Weevil [http://www.thaibugs.com/?page_id=284 © Thaibugs]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
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The Mango Pulp Weevil (Sternochetus frigidus or Cryptorrhynchus gravis) has been observed in Bangladesh, India, Indonesia, Malaysia, Myanmar,&lt;br /&gt;
Pakistan, Papua New Guinea, Philippines, Singapore and Thailand. This female weevil lays eggs (25-60 eggs / week) on mango fruits (minimum 6 cm diameter). Newly hatched larvae tunnel into the fruit pulp. The larvae form a chamber (constructed of frass) next to the seed, from which they eat their way through the pulp. Pupation occurs within these chambers. Matured weevils leave the ripe fruit through an exit hole in the peel. Prior to this exit, nothing shows that the fruit is infected. They are fully matured after 6 weeks; able to mate, repeatedly. One complete life cycle varies from 34-35 days. More than half of adult weevils hibernate in seeds. Mango Pulp Weevils are very poor flyers (maximum 90 cm horizontally).[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Thrips==&lt;br /&gt;
[[Image:Frankliniella_occidentalis.jpg|thumb|left| F. occidentalis [http://conabio.inaturalist.org/taxa/243761-Frankliniella © Th0th]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Thrips, or thunderflies, thunderbugs, storm flies, thunderblights, storm bugs, corn flies or corn lice may feed on many plants. Frankliniella bispinosa, Frankliniella kelliae (Florida, Caribbeans) and Frankliniella occidentalis (California, Israel) also feed on mango. They feed on young leaves, shoots and flowers, causing discoloration and deformation.&lt;br /&gt;
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Thrips are tiny (1 mm long on average; varying from 0.5 mm to 1.4 cm). They are not good flyers, but may readily be carried by the wind. Given the right conditions, they may rapidly multiply and form large swarms. To inspect trees for thrips infestation, just hold a bucket and shake a branch, and some thrips will fall off the infested branch into the bucket.&lt;br /&gt;
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Control: Natural enemies of thrips are Green lacewings (eg Mallada boninensis, Mallada signata), Orius insidiosus, Anystis agilis and Hypoaspis aculifer. Biological insecticides such as Beauveria bassiana and Verticillium lecanii may be effective.&lt;br /&gt;
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[u]Orius insidiosus[/u] is a 3 mm long pirate bug (aka insidious flower bug). They feed on thrips, mites, small caterpillars and various insect (including aphids&amp;#039;) eggs and larvae, and also pollen. They prefer eating thrips over aphids.[http://www.ncbi.nlm.nih.gov/pubmed/18845007] Orius may kill several thrips per day, beyond their need for nutrients. Adults fly well, and may readily disperse, looking for prey. O. insidiosus may be mass reared on [http://www.waiwiki.org/index.php?title=Armyworm armyworms]. They may occasionally sting people, which may hurt, but is completely harmless. Eggs are laid and embedded in the plant tissue of the fruit, stem, leave stalks and big veins at the underside of leaves. From eggs to adults, Orius goes through 5 larval stages. This takes about 16 to 18 days at 25°C. They are predatory at each stage (except as pupae). Adults live 3 to 4 weeks. As Orius albidipennis lays more eggs and has a shorter lifecycle, it may be a better biocontrol agent than Orius insidiosus.[http://eurekamag.com/research/030/621/030621437.php] Release at 2 to 3 Orius per square meter.&lt;br /&gt;
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==Whiteflies==&lt;br /&gt;
[[Image:Trialeurodes_vaporariorum.jpg|thumb|left| Trialeurodes vaporariorum [http://en.wikipedia.org/wiki/File:Weisse-Fliege.jpg © Gaucho]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| Orius insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Paraleyrodes_bondar.jpg|thumb|left| Paraleyrodes bondar [http://manateeornprod.wordpress.com/2012/02/22/new-whitefly-in-florida/ © UF/IFAS]]]&lt;br /&gt;
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Whiteflies (incl. Aleurodicus rugioperculatus, Aleurodicus dispersus, Aleurothrixus floccosus, Aleyrodes proletella, Bemisia tabaci, Paraleyrodes bondar, Siphoninus phillyreae, Trialeurodes vaporariorum) attack various crops, including mango, orange, tomato and watermelon. &lt;br /&gt;
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Whiteflies suck the saps from leaves, causing direct damage. They are also vectors of various diseases, including viruses. Additionally they may produce a lot of honeydew, which may lead to sooty mould. The density of the whitefly is positively correlated with maximum temperature and negatively correlated with relative humidity.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Natural enemies[/u]; Various animals predate on whiteflies, including green lacewings, Orius insidiosus and ladybird beetles. The minute parasitic wasps Encarsia guadeloupae and Encarsia haitiensi very effectively cause reduction in the population of Aleurodicus despersus.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Control[/u]&lt;br /&gt;
Dissolve 1 kg of sugar per 1 L of water. Poor in plastic transparent bottle. Drill holes in the upper part (above water level); small enough for whiteflies to enter, but not for bees. Put the lid on. Hang 2 to 3 bottles in each tree at flower set. Renew weekly.&lt;br /&gt;
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=Integrated Pest Management=&lt;br /&gt;
Integrated Pest Management (IPM) integrates proper orchard managment (such as sufficient spacing of trees, pruning off overlapping branches, the use of bait traps and removing affected fruits and plant parts) and the use of a variety of anti-bugs (natural enemies of mango bugs), so that they are complementary and sufficiently reduce all pest populations, minimizing the use of pesticides.&lt;br /&gt;
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Below is an example of the complementary use of anti-bugs, for both predation and parasitism.&lt;br /&gt;
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Preliminary requirements: A sealed, ventilated room. Constant supply of mangoes, or grapes, kiwi, lychee, pineapple, peach, apricot, papaya, apple or pear, to feed and rear Drosophilae (2-4mm long &amp;#039;fruit flies&amp;#039;), Tephritids (4-7 mm long &amp;#039;true&amp;#039; fruit flies) and Eudocima sp. (fruit piercing moths; up to 10 cm wingspan). One section needs daylight exposure, for growing Fabacae / Menispermacae for the Eudocima sp. to lay their eggs, and for their larvae to feed on the leaves. This sealed room should rather be too large than too small to prevent a lack of food for the reared predators. Any surplus of eggs and/or larvae may be sold as fish feed.&lt;br /&gt;
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* Rearing [http://www.waiwiki.org/index.php?title=Praying_mantis Praying Mantids] to predate on moths, caterpillars, weevils and young Longicorn beetles by adults, and on fruit flies, hoppers and midgets by young mantids. These mantids may be reared on fruit flies  (Drosophila and Tephretids) and specific moths. Young mantids should be separated after hatching, in boxes. Nymphs should be fed Drosophilae. Young mantids should be fed Tephretids. Adult mantids are fed Eudocima moths or Armyworm moths.&lt;br /&gt;
* Rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings] (Mallada signata, Mallada boninensis) to predate on aphids, scales, hoppers and thrips in the field. These lacewings may be reared on Eudocima sp. eggs collected from the Fabacae / Menispermacae in the sealed room, or on Armyworm eggs. &lt;br /&gt;
* Rearing the parasitic wasps Fopius arisanus and/or Diachasmimorpha longicaudata to parasitize Tephritids (fruit flies). Tephritid eggs should be collected from fruits in the sealed room. To prevent the fruit fly eggs from drying out, the eggs should be kept on a moist substrate. To prevent molding, a constant air current should be provided during the time required for parasitoid development.&lt;br /&gt;
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The second phase may be more labour intensive. To rear Tetrastichus sp. and Euplectrus sp. or Trichogramma sp. for the purpose of parasitizing scales, moths, midges and longicorn beetles, one most collect eggs from all species in the field. Instead, one may also rear subject pests.&lt;br /&gt;
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As an alternative for green lacewings, one may use the combination of Cryptolaemus montrouzieri (mass reared on Plancoccus citri on potatoes or squash [http://www.arabscientist.org/english/page/11/], for predating on scales) and Orius insidiosus (mass reared on [http://www.waiwiki.org/index.php?title=Armyworm Armyworms] (which are best reared on Bermudagrass), for predating on aphids, thrips and small caterpillars). This allowes for a more targeted approach (if there is only 1 pest, eg scales). This may alsobe a better combination (together with Praying mantids) because adult Praying mantids prefer to predate on lacewings (15 mm long) over the much smaller C. montrouzieri (4 mm) and O. orius (3 mm).&lt;br /&gt;
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=Diseases=&lt;br /&gt;
Bugs cause damage to the mango tree, which makes the tree more susceptible to disease. Many bugs are also disease vectors; they transport the viruses, fungi etc. to the mango tree. Successfully combatting mango bugs, is therefore key to prevention and control of mango diseases. Using chemicals to fight mango diseases and/or bugs, however, may actually result in killing natural enemies of the bugs that spread disease.&lt;br /&gt;
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==Anthracnose==&lt;br /&gt;
[[Image:Anthracnose.jpg|thumb|left| Anthracnose [http://www.indiancropdiseases.com/mango.aspx]]]&lt;br /&gt;
[[Image:Anthracnose-2.jpg|thumb|right| Anthracnose [http://hawaiiplantdisease.net/cpg/displayimage.php?pid=397 © Nelson]]]&lt;br /&gt;
Anthracnose is caused by the fungi Colletotrichum acutatum [http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2003.93.5.579] and Glomerella cingulata [http://www.idosi.org/wjas/wjas4(6)/8.pdf][http://www.jofamericanscience.org/journals/am-sci/am0608/46_3350am0608_361_367.pdf]. It particularly affects young shoots, flowers and fruits. The disease is stimulated by high humidity, frequent rains or mists, and a temperature range of 24 to 32°C. The results of this disease are black spots on panicles, leaf spot, blossom blight, withered tip, twig blight and fruit rot. Particulary young and tender shoots and foliage are affected, causing die back of young branches. Wounded twigs may also be infected and may sometimes die off. Fruits develop black spots and rotting and young fruits may shrivel and prematurely drop. If the entire inflorescence is destroyed, no fruits will set. &lt;br /&gt;
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[u]Where?[/u] It widely occurs in the orchard and in storage, Anthracnose has been reported in Argentina, Florida, Hawaii, India, Pakistan, Philippines, Sri Lanka, South Africa and Trinidad.&lt;br /&gt;
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[u]Control[/u]: Burn all fallen leaves. Prune affected twigs and burn them. Apply Bordeaux paste on cut ends. To prevent blossom infection, spray twice at 15 days interval during flowering with Carbendazirn (Bavistin 0.1%). To prevent foliar infection, spray copper fungicides (0.3%) twice a week. Apply a teaspoon of dish detergent per spray load to make the copper sulfate stick. Prevent the copper sulfate from dripping on the ground to prevent contamination of the soil and the roots of the tree.&lt;br /&gt;
Picked mangoes should be submerged for 15 minutes in hot water (52°C) with Carbendazim (0.1%). One may also dip the mangoes in 500 ppm Benomyl and 900 ppm Thiobendazole. C. acutatum is also effectively inhibited by the synthetic strigolactone GR24.[http://www.ncbi.nlm.nih.gov/pubmed/21688170]&lt;br /&gt;
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==Bacterial Canker==&lt;br /&gt;
Bacterial black spot (bacterial canker) is a bacterial disease caused by Xanthomonas campestris pv. mangiferaeindicae. It affects many varieties. Initially, irregular small water soaked lesions will appear on the leaves. These will extend into patches of dead tissue. The leaves will turn yellow and die. The lesions will also appear on the the stalk of the leaves, twigs and fruits. The fruits will then turn brown and black, and burst open. The released fluid is filled with bacteria that will contaminate the rest of the tree or other fruits in storage.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Agrimycin-100 (0.01%) or Streptocycline (0.01%), 3 times at 10 days interval. Subsequently spray Copper Oxychloride (0.3%) or Carbendazim (Bavistin 0.1%) once a month.&lt;br /&gt;
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==Die Back==&lt;br /&gt;
Die back (Botryodiplodia (Lasiodiplodia) theobromae aka Physalospora disrupta/quercuum/rhodina/glandicola) may occur any time of the year. It is characterized by advancing discoloration and darkening of the bark. It extends along the veins of the leaves, causing browning and upwards rolling of the margins. Eventually the leaves shrivel and fall. Twigs and branches dry and defoliate, and may drain a yellow-brown gummy fluid. It may look as if the tree has been exposed to a bush fire.&lt;br /&gt;
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[u]Control[/u]: Prune diseased twigs 10 cm below the affected area. Paste the cut ends with Copper Oxychloride (0.3%) and also spray Copper Oxychloride (0.3%) on affected trees.&lt;br /&gt;
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==Diplodia Stem-end Rot==&lt;br /&gt;
This fungus (Lasiodiplodia theobromae) may enter the mango where the skin or stem has been injured mechanically. It will form a black circle around the pedicel.&lt;br /&gt;
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[u]Control[/u]: Prevent mechanical injuries. After picking, submerge mangoes for 15 minutes in hot water (53°C) with Carbendazirn (0.1%).&lt;br /&gt;
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==Mango Black Blight==&lt;br /&gt;
Black blight is caused by a fungus (Capnodium mangiferum).&lt;br /&gt;
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==Mango Blight==&lt;br /&gt;
Mango blight is caused by Erwinia bacteria (Enterobacteriaceae). Many spots appear on the leaves which cause a reduction in growth and yield. &lt;br /&gt;
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[u]Control[/u]: Use Dithane M 45 at the rate of 1.7g/L (450 L water per acre).&lt;br /&gt;
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==Mango Malformation==&lt;br /&gt;
Mango malformation (Fusarium mangiferae) has been reported in Australia, Bangladesh, Brazil, Central America, Cuba, Egypt, India, Israel, Malaysia, Mexico, Pakistan, South Africa, Sudan, Switzerland, UAE and the USA. It is a disease caused by fusarium species [http://www.mycologia.org/content/94/4/722.full][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.6.456] (F. subglutinans from Egypt, Florida, Israel, Malaysia and South Africa, F. sterilihyphosum from Brazil and South Africa, and Fusarium sp. nov. and F. proliferatum from Malaysia)[http://www.ncbi.nlm.nih.gov/pubmed/18943188][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-96-0667 Free Full Text]. The disease is stimulated by relatively cool conditions; 10 to 27°C, and merely survive hotter conditions. In this disease the leaves and inflorescence of the mango tree are badly deformed and gradually dry up. There is no fruit setting and hence no production. &lt;br /&gt;
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[u]Control[/u]: Various fungicides, including Captan, Benomyl and Thiram have been reported to be effective to some extend. &lt;br /&gt;
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==Mango Scabs==&lt;br /&gt;
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Mango Scabs (Denticularia mangiferae aka Sphaceloma mangiferae aka Elsinoë mangiferae) survives on young tissues (young leaves, shoots, flower, fruits) of the mango tree only. Adult tissue is not susceptible. Its spores (and their germination) requires rain splash and free water to produce new infections.[http://www.cerambycoidea.com/titles/affa2004.pdf] Moisture conditions stimulate its growth. Though this parasite is not a fungus but in a persistent parasytic relationship with the mango tree, it produces symptoms that look somewhat familiar to those of anthracnose (a fungus) infections. It starts with small light- or dark brown or grey spots on the underside of leaves and fruit. These spots get bigger and darker with time. In the center of this lesion a cracked texture will appear. If the host tissue survives, lesions may develop into scar tissue. When infestation is severe, there is loss of leaves and fruit.&lt;br /&gt;
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[u]Control[/u]; Remove and destroy all fallen leaves, twigs, branches etc. Spray copper hydroxide or copper oxychloride on a very regular basis, particularly in the rainy season. Without chemical control, losses as high as 90% have been observed.[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Phoma Blight==&lt;br /&gt;
Phoma blight (Phoma glomerata) is a fungus that affects the fibre in the top layer of old leaves. It produces small, irregular, angular lesions. As the lesions grown, the colour changes from yellow-brown to cinnamon. Many small spots may form overwhelming patches and result defoliation of affected leaves. Phoma glomerate very effectively produces an enzyme that converts the remainders (glucose) of fibre (from the leaves) into it the main structural component (N-acetyl-D-glucosamine)[http://www.ncbi.nlm.nih.gov/pubmed/15553782] in its cell walls, thus enabling its own growth.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Benomyl (0.2%), and 20 days later spray 0.3% Miltox (Copper Oxychloride plus Zineb). Quadris (azoxystrobin) combined with thymol at a non-fungitoxic concentration produces much higher growth inhibition of Phoma glomerata than the fungicide alone.[http://www.ncbi.nlm.nih.gov/pubmed/22408641]&lt;br /&gt;
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==Powdery Mildew==&lt;br /&gt;
Powdery mildew (Oidium mangiferae) affects virtually all mango varieties. Characteristically, a white superficial powdery fungal grows on the inflorescence and tender leaves, stalk of panicles, flowers and young fruits. The affected flowers and fruits will pre-maturely drop, or fruit setting does not occur at all. The disease is initiated and developed by warm (&amp;gt;17°C) dry weather, but during the flowering stimulated by prolonged rains or mists and by cool nights. The disease has been reported in Brazil, India, Jamaica, Pakistan, Sri Lanka, South Africa and the U.S.A. &lt;br /&gt;
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[u]Control[/u]: Alternately spray wettable sulphur (0.2% ; 2 g Sulfex/L), Tridemorph (O.1% ; 1 ml Calixin/L) and Bavistin (0.1%) at 15 days interval. Start spraying when the panicle (flower cluster) starts to emerge. Or spray with Carbendazim (0.1%) or Tridemefon (0.05%) or wettable sulphur (0.3%) alone (3 days in a row).&lt;br /&gt;
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[u]Control-2[/u]: Similar to A. quisqualis AQ10 Biofungicide in commercial use for biocontrol of powdery mildew, Phoma glomerata (see [http://www.waiwiki.org/index.php?title=Mango#Phoma_Blight Phoma Blight] above) may act as mycoparasite of powdery mildew. Phoma glomerata can colonize and suppress development of powdery mildew.[http://www.ncbi.nlm.nih.gov/pubmed/10618259] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91841/ Full free text]&lt;br /&gt;
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==Red Rust==&lt;br /&gt;
Red rust (Cephaleuros virescens aka C. parasiticus) initially causes round and slightly elevated green-grey spots mainly on leaves. They turn into rusty red spots and may form bigger patches. This reduces the photosynthetic capacity of the leaves. In severely infected trees, twigs will remain stunted, the bark and twigs will get thick and the leaves will dry up and drop off. &lt;br /&gt;
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[u]Control[/u]: Spray Copper Oxychloride (0.3%) 3 times.&lt;br /&gt;
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==Sooty Mould==&lt;br /&gt;
Sooty mould (Meliola mangiferae) may be the result of too many insects in your orchard. Scale insects, mealy bug and hoppers secrete honey dew. The honey dew sticks to the leaves, feeding fungal growth. This results in a black sooty mould over the entire surface of leaves and twigs, affecting the photosynthetic capacity of the leaves. Uncontrolled, the tree may completely turn black.&lt;br /&gt;
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[u]Control[/u]: Prune and burn the affected branches. Spray Wettasulf (0.2%) with+ Metacid (0.1 %) and gum acacia (0.3%). Ants feed on the honeydew excreted by soft scales, preventing accumulation of sooty moulds, but they also protect the scales from natural enemies.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
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	<entry>
		<id>http://www.waiwiki.org/index.php?title=Mango&amp;diff=4216</id>
		<title>Mango</title>
		<link rel="alternate" type="text/html" href="http://www.waiwiki.org/index.php?title=Mango&amp;diff=4216"/>
		<updated>2014-12-11T18:02:26Z</updated>

		<summary type="html">&lt;p&gt;Aytundra: Spelling&lt;/p&gt;
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&lt;div&gt;Comparable to oranges and clementines, mangoes are among the most all-round fruits, regarding nutrients. Mangoes are cultivated in (sub)tropical regions all over the world.&lt;br /&gt;
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=Low-fibre, sweet mango species=&lt;br /&gt;
If you use a juice extractor like the [http://www.thekitchn.com/product-review-hurom-slow-juic-131492 Hurom] / [http://versapers.fr/ Versapers] (masticating slow juicer), the stringy fibres from species like Tommy Atkins or Haden will clog your juicer (more so than pineapples). The chamber will fill up with long fibres that simply never get discarded, so that you need to empty and clean it after every few mangoes. With mangoes like Kent (#11 on the list), you may endlessly (&amp;gt;100 mangoes) continue juicing without having to empty or clean the chamber in between, because the short fibres are immediately discarded as pulp (as with apples, pears, clementines).&lt;br /&gt;
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==Anwar Ratol==&lt;br /&gt;
[[Image:Anwar_Ratol.jpg|thumb|right| Anwar Ratol [http://freshfruitpakistan.blogspot.nl/2013/04/anwar-ratol-top-variety-of-mango.html © H. Khalid]]]&lt;br /&gt;
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* #1 (aka Anwer Rathol, Anwar Retaul or Anwar Rataul)&lt;br /&gt;
* Small size (av. 180 g), medium thick skin&lt;br /&gt;
* Matures from green to pale bright yellow &lt;br /&gt;
* Very sweet (TSS 26-28%)&lt;br /&gt;
* Fibreless flesh, medium juicy&lt;br /&gt;
* Originally from Rataul, in Bagpat district, in the province of Uttar Pradesh, India. &lt;br /&gt;
* Harvested from June to July (India and Punjab in Pakistan)&lt;br /&gt;
* Keeps well in storage&lt;br /&gt;
&lt;br /&gt;
==Alphonso==&lt;br /&gt;
[[Image:Alphonso.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #2 (aka Alphanso, Bombay)&lt;br /&gt;
* Small, roundish / oblong (av. 200 g.)&lt;br /&gt;
* Smooth, (from green to) pale greenish-yellow to deep orange peel (deepening with ripening)&lt;br /&gt;
* Smooth, firm flesh. Intense reddish-orange colour&lt;br /&gt;
* Sweet, somewhat sour (17% sugar; sugar/acid ratio: 39; TSS 21-23%) and flavoured&lt;br /&gt;
* Virtually without fibre (1%), medium juicy&lt;br /&gt;
* Originally from Western India&lt;br /&gt;
* Main production in Maharashtra and Gujarat&lt;br /&gt;
* Highest quality in Sindhudurg, Ratnagiri (Natwarlal plantation), Raigad and Thane districts&lt;br /&gt;
* Harvested from early April to early July (India) and July (some districts in Sindh, Pakistan)&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
* Tolerates high humidity&lt;br /&gt;
* Good keeping quality (up to 4 weeks at 11° C)&lt;br /&gt;
&lt;br /&gt;
==Dashehari==&lt;br /&gt;
[[Image:Dashehari.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #3 (aka Dasheri, Doshehri, Aman Dusehri or Dussehri)&lt;br /&gt;
* Small, oblong (av. 175 g)&lt;br /&gt;
* Matures from light green to light yellow, thin skin&lt;br /&gt;
* Sweet (TSS 21-22%), lemon yellow flesh&lt;br /&gt;
* Fibreless, medium juicy&lt;br /&gt;
* Very small stone&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Dasheri, Uttar Pradesh, India.&lt;br /&gt;
* Harvested from early June to July, mainly Malihabad, Uttar Pradesh (and Punjab)&lt;br /&gt;
&lt;br /&gt;
==Bagan Pali==&lt;br /&gt;
[[Image:Bagan_Pali.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #4 (aka Begun Palli, Began Phali, Banganapalli or Baganpali)&lt;br /&gt;
* Large (av. 550 g.), oval&lt;br /&gt;
* Sweet (TSS 18-20%)&lt;br /&gt;
* Fibreless, little juicy, yellow flesh &lt;br /&gt;
* Matures from dark green to yellow light green or yellow-orange&lt;br /&gt;
* Thin, smooth, shiny skin&lt;br /&gt;
* Originally from Sindh, Pakistan and/or Banganapalle in Andhra Pradesh, India.&lt;br /&gt;
* Harvested from July to August (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Sindhri==&lt;br /&gt;
[[Image:Sindhri.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #5 (aka Sindhry)&lt;br /&gt;
* Medium to large (av. 300 g., av. 400 g in Pakistan)&lt;br /&gt;
* Elongated with pointy curve&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 36; TSS 15-17%), flavoured&lt;br /&gt;
* Little fibre (3% - 5%), little juicy&lt;br /&gt;
* From green to deep matt lemon yellow thin peel (somewhat wrinkly) when ripe&lt;br /&gt;
* Deep yellow-orange flesh; soft and melting&lt;br /&gt;
* Originally from Mir Pur Khaas in Sindh&lt;br /&gt;
* Harvested from late May to July (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Chaunsa==&lt;br /&gt;
[[Image:Chaunsa.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #6 (aka Sammar Bahisht Chausa)&lt;br /&gt;
* Large (av. 350 g.), oblong&lt;br /&gt;
* Sweet (18% sugar; sugar/acid ratio: 58; TSS 18-20%)&lt;br /&gt;
* Soft, succulent flesh &lt;br /&gt;
* Medium fibre, large stone, medium juicy (delicious for eating, too stringy for the slow juicer)&lt;br /&gt;
* Thin, pale, matt yellow-greenish peel (wrinkly and slightly deeper when ripe)&lt;br /&gt;
* Heavy bearer&lt;br /&gt;
* Originally from Pakistan&lt;br /&gt;
* Harvested mainly in the Rahim Yar Khan and Multan (Sahiwal) districts of Punjab in Pakistan&lt;br /&gt;
* Harvested from June to early September (Pakistan)&lt;br /&gt;
&lt;br /&gt;
==Maya==&lt;br /&gt;
&lt;br /&gt;
* #7&lt;br /&gt;
* Medium, round (av. 300 g.)&lt;br /&gt;
* Sweet (17% sugar; sugar/acid ratio: 42; TSS 16-17%) and flavoured&lt;br /&gt;
* Red blush peel (with yellow tinge when ripe)&lt;br /&gt;
* Smooth, deep/pale yellow flesh (soft when ripe)&lt;br /&gt;
* Medium fibre, very juicy&lt;br /&gt;
* Harvested from mid-June to early July (Gambia)&lt;br /&gt;
&lt;br /&gt;
==Gopalbogh==&lt;br /&gt;
&lt;br /&gt;
* #8 (aka Gopal Bhogue)&lt;br /&gt;
* Small size (average 190 g.)&lt;br /&gt;
* Yellow-green peel, with small seeds&lt;br /&gt;
* Virtually without fibre&lt;br /&gt;
* Very sweet (21% sugar)&lt;br /&gt;
* Good quality mainly grown in Rajshahi region, especially in Chapainawabganj district (Bangladesh).&lt;br /&gt;
* Harvest from mid May to mid June (Bangladesh)&lt;br /&gt;
&lt;br /&gt;
==Sweet Elena==&lt;br /&gt;
[[Image:Sweet_Elena.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #9&lt;br /&gt;
* Large sized, ovoid (av. 350 g.)&lt;br /&gt;
* Thin, orange peel&lt;br /&gt;
* Yellow to orange flesh&lt;br /&gt;
* Little fibre&lt;br /&gt;
* Sweet (19% sugar)&lt;br /&gt;
* Originally (as a carabao variety) from Sta. Cruz, Zambales (Philippines)&lt;br /&gt;
* Harvest from March to April (Locloc, Palauig in northern Zambales, Philippines)&lt;br /&gt;
&lt;br /&gt;
==Ataulfo==&lt;br /&gt;
[[Image:Ataulfo.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #10 (aka Adaulfo, Adolfo, Champagne, Manila or honey mango, related to Alphonso)&lt;br /&gt;
* Small, oblong shape (av. 225 g.)&lt;br /&gt;
* With ripening, peel turns from pale green to gold-yellow (yellow to orange and wrinkles when fully ripe)&lt;br /&gt;
* Deep yellow flesh (gold when ripe), little fibre and thin pit&lt;br /&gt;
* Smooth, sweet (15% sugar), and buttery / creamy flesh&lt;br /&gt;
* Spicy flavour &lt;br /&gt;
* Best temperature is 28°C, tolerates 1100 to 3000 mm annual rainfall &lt;br /&gt;
* Originally from Chiapas, Mexico&lt;br /&gt;
* Harvest from mid-October to late December (Ecuador); February to August (Ghana)&lt;br /&gt;
* May be stored in the fridge for 2 weeks&lt;br /&gt;
&lt;br /&gt;
==Kent==&lt;br /&gt;
[[Image:Kent.jpg|thumb|right|]]&lt;br /&gt;
&lt;br /&gt;
* #11&lt;br /&gt;
* Large, rounded oval (500 to 800 g.)&lt;br /&gt;
* Smooth, waxy thick peel (green with some yellow and red; yellow increases with ripening)&lt;br /&gt;
* Soft, melting, juicy flesh (from deep yellow to deep orange when fully ripe)&lt;br /&gt;
* Limited fibre (dry weight: 0.4 - 2%), small seed&lt;br /&gt;
* Sweet (13% sugar; 14ºBrix [http://www.m.elewa.org/JAPS/2013/17.3/5.pdf])&lt;br /&gt;
* Originally from Mexico / Florida&lt;br /&gt;
* Harvest from January to March and May to August (Ghana)&lt;br /&gt;
* Large tree (up to 20 m.)&lt;br /&gt;
* No storage keeping below 13°C&lt;br /&gt;
* Fairly disease resistant&lt;br /&gt;
&lt;br /&gt;
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&lt;br /&gt;
=Mango Cultivation=&lt;br /&gt;
==Conditions==&lt;br /&gt;
The best soil for mango trees has a pH between 5.5 and 7.5 (high pH may cause leaf tip burn). The temperature range for growing mangoes is 15 to 40°C. Mango does best within a temperature range of 24 to 27°C. The Indian race is in general intolerant of humidity (susceptible to anthracnose), the Philippine race tolerates excess moisture. The life span of each tree is approximately 100 years. Economic bearing life is 30 to 50 years.&lt;br /&gt;
Mango trees need deep soil (up to 2 m) for their root systems. Grafted saplings yield a more superior quality of fruit. Fruits are borne by mango trees from 4 to 5 years after planting. Full bearing starts at 6 to 7 years.&lt;br /&gt;
Grafted mango plants start bearing blossoms one to two years after planting, provided that there is no high humidity or intense rain during the flowering period (dryness induces flowering).&lt;br /&gt;
&lt;br /&gt;
==Rootstock Propagation==&lt;br /&gt;
[[Image:Mango_grafting.jpg|thumb|left| [http://www.muftisays.com/blog/ummi+taalib/2718_20-03-2012/shaikhmurid-relationship.html © ummi taalib]]] &lt;br /&gt;
[[Image:Grafted_seedling.jpg|thumb|centre| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
[[Image:Grafted_seedlings.jpg|thumb|right| [http://materialsandmethodsofgrafting.blogspot.nl/ © Boopaloo]]]&lt;br /&gt;
Trees grown from mango seeds (and naturally, sexually propagated) will be very different from the tree those mangoes came from (such &amp;#039;wild mangoes&amp;#039; usually taste bad). That is why rootstocks (raised from seeds) are asexually propagated. Matured seedlings (about 60 cm tall) are grafted by inarching; a bud from a tree producing good quality mangoes is artificially attached to the rootstock. This will create a new tree with two parts: the lower (wild) part and the upper part, which is an exact copy of the tree you took this bud from. Any bud growing from the lower part needs to be pruned off immediately. The upper part needs to grow and produce branches, and eventually fruits. During the first month after planting, the young trees need to be irrigated every 3 days. During the next 2 months, they need to be irrigated once a week. After that they should not be watered for a couple of months. Each year they need a dry season of a few months. Young plants need 10 to 30 kg organic manure per plant. Don&amp;#039;t use a chemical fertilizer on young plants. Once the tree is 2 years old, you may start using a controlled release fertilizer (with higher nitrogen), like 12-5-9 or 12-8-34 (= ratios N:P:K); 1 tbsp. in 1 L warm water / meter tree height. Or just manure.  &lt;br /&gt;
&lt;br /&gt;
Rootstock branches should always be pruned off. To prevent viral infections, only prune using a very sharp, sterilized knife (sterilize on the spot with a propane torch).&lt;br /&gt;
&lt;br /&gt;
==Production technology== &lt;br /&gt;
Space mango trees about 12 meters apart. Adult mango trees need about 500-750 mm of water per year. In the wet season irrigated once per 8 to 10 days. In the dry season once per 15 to 20 days. Mango trees need dry and wet spells alternately (also kills various pathogens). Don&amp;#039;t fertilize mature trees during fruit bearing, and not too much irrigation. If during the blooming season the soil contains much nutrients (fertilizer) and water, the tree will grow, but not flower and bear fruits. &lt;br /&gt;
You may either:&lt;br /&gt;
* A: Apply 80 to 100 kg manure per year (eg humanure from [http://www.waiwiki.org/index.php?title=Composting_toilet composting toilets]), after all fruits are picked. Or:&lt;br /&gt;
* B: Apply 3-4 kg SSP, 2-3 kg Potassium Sulphate and 2-3 kg Urea before flowering. Plus 2-3 kg Urea in 2 doses after fruit setting. Or:&lt;br /&gt;
* C: Use a controlled release fertilizer once a year, such as 4-4-8 (= ratios N:P:K), after all fruits are picked (1 tbsp. in 1 L warm water / meter tree height).&lt;br /&gt;
 &lt;br /&gt;
After fruit harvest, prune off diseased, dried, broken branches and those touching the ground. Every 3 to 4 years about 15 to 20% of old wood should be removed.&lt;br /&gt;
Picking should be done when the fruit is fully developed and mature. Natural drop of the fruit is the main indication that the fruit is ready for picking. Expected yields vary from 40 to 100 kg per tree.&lt;br /&gt;
&lt;br /&gt;
One cannot rear any animals (except for carnivores) on land with mango trees, as mango leaves are deadly poisonous.&lt;br /&gt;
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&lt;br /&gt;
=Bugs=&lt;br /&gt;
&lt;br /&gt;
==Aphids==&lt;br /&gt;
[[Image:Propylea_quatuordecimpunctata.jpg|thumb|right| Predatory ladybird [http://www.colpolon.biol.uni.wroc.pl/propylea%20quatuordecimpunctata.htm]]]&lt;br /&gt;
[[Image:Aphids_on_mango.png|thumb|left| Aphids [http://www.infonet-biovision.org/default/ct/124/crops#_1804_1202 © Varela]]]&lt;br /&gt;
[[Image:Mallada_sp_2.jpg|thumb|right| Mallada sp. © Texas University]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
&lt;br /&gt;
Aphids (plant lice; Toxoptera odinae) are small (1-4 mm), living in clusters. They pierce plant tissue and suck the sap of the leaves, which may cause the leaves to bend, twist or roll up. Aphids also transmit plant viruses. Some species produce winged offspring, &amp;quot;alates&amp;quot;, that may readily disperse. Dry (and warm) weather stimulates increases in aphid numbers. Some species of ants (eg Dolichoderus cuspidatus and Formica aerate) protect and &amp;#039;farm&amp;#039; aphids, feeding on the honeydew released by the aphids&amp;#039; alimentary (gut) canals. Insects secreting honeydew may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould] &lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Plant flowering plants at the boarders to attract beneficial insects. Plant &amp;#039;trap crops&amp;#039; (dill, nasturtiums, timothy grass) to monitor aphid numbers. Check the trap crops every 3 days. Infested trap crops need to be burned. Aphid&amp;#039;s natural enemies include predatory ladybirds (eg Propylea quatuordecimpunctata), hooverfly larvae, parasitic wasps, aphid midge larvae, crab spiders (Thomisidae) and lacewings (Neuroptera). Exposing aphid populations to natural predators may be successful particularly when its hot, because once bitten they release an alarm pheromone and the others all jump off the plant. Jumping aphids may experience a heat shock (&amp;gt; 35°C) since the ground may be much warmer than the shady tree. This heat shock usually sterilizes the aphids (killing the bacteria on aphids that provide nutrients essential for aphid reproduction). [http://www.sciencedaily.com/releases/2007/04/070419172046.htm]&lt;br /&gt;
&lt;br /&gt;
[u]Crab spiders (Thomisidae)[/u]; Crab spiders are ambush hunters (they don&amp;#039;t build webs) that not just eat aphids, but may also hunt ants that farm aphids. Some of these crab spiders, Xysticus sp, may however not just eat ants, but also predatory beetles (that eat aphids).&lt;br /&gt;
&lt;br /&gt;
[u]Green lacewings (Chrysopidae)[/u]; One may buy millions of lacewings (as captive-bred eggs) for biological pest control, as reared for that purpose in many countries. A female lacewing may plant over 100 eggs on plants infested with aphids. These eggs hang on a thin 1 cm long stalk, most often under a leaf. The larvae of most species of lacewings are specialised predators that eat aphids (and coccids such as mango scales, and caterpillars). These larvae sway their heads from left to right, and back, until they strike something they can eat. They have mouthparts that can pierce and suck the aphids. Many (adult) lacewing species also feed on nectar and pollen, but particularly Chrysopidae (eg Mallada signata, Mallada boninensis) are mainly predatory (one adult may eat 15 aphids per day). One may attract Chrysopidae by planting crops that attract them (mainly Asteraceae and Apiaceae), such as calliopsis (Coreopsis), cosmos (Cosmos), sunflowers (Helianthus), dandelion (Taraxacum), dill (Anethum) and angelica (Angelica). &lt;br /&gt;
&lt;br /&gt;
[u]Chemicals[/u]; If you have no other option and immediate action is required: Use Folido 50% EC at the rate of 0.45 L per 450 L water / acre. (also kills natural aphid enemies)&lt;br /&gt;
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==Fruit Flies==&lt;br /&gt;
[[Image:Fruit_fly_maggots.jpg|thumb|centre| Maggots [http://www.infonet-biovision.org/default/ct/124/crops © A. M. Varela]]]&lt;br /&gt;
[[Image:Bactrocera_invadens.jpg|thumb|left| B. invadens [https://www.flickr.com/photos/iaea_imagebank/6776806998/ © IAEA]]]&lt;br /&gt;
[[Image:Fopius_arisanus.jpg|thumb|right| F. arisanus [http://www.tephritid.com/digital.php?act=page&amp;amp;pid=28&amp;amp;id=25 © tephritid.com]]]&lt;br /&gt;
[[Image:Ceratitis_capitata.jpg|thumb|left| C. capitata [http://josedotinzulza.edublogs.org/2013/04/21/how-much-damage-can-make-a-fly/ © José Inzulza]]]&lt;br /&gt;
[[Image:Diachasmimorpha_longicaudata.jpg|thumb|right| D. longicaudata [http://www.oocities.org/brisbane_parawasps/FruitFlyParasitoid.htm]]]&lt;br /&gt;
[[Image:Ceratitis_fasciventris.jpg|thumb|left| C. fasciventris [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Oecophylla_longinoda.jpg|thumb|right| O. longinoda [http://www.alexanderwild.com/Ants/Taxonomic-List-of-Ant-Genera/Oecophylla/i-tMfJXKG © A.Wild]]]&lt;br /&gt;
[[Image:Ceratitis_cosyra.jpg|thumb|left| C. cosyra [http://www.infonet-biovision.org/print/images/93/pests © Copeland]]]&lt;br /&gt;
[[Image:Jackson_trap.jpg|thumb|right| Jackson trap]]&lt;br /&gt;
[[Image:Ceratitis_rosa.jpg|thumb|left| C. rosa [http://wzciq.wenzhou.gov.cn/art/2011/1/12/art_6811_63595.html © wzciq]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis eating fly [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
In 2013, the export of mangoes from Ghana and 27 other African countries was banned from major international markets mainly due to fruit fly infestation. The European Union imposed a ban from May 1 2014, on import of mangoes from India, also due to fruit fly infestation. &lt;br /&gt;
&lt;br /&gt;
The fruit flies (aka peacock flies; 4-7 mm long) that may form a very severe mango cultivation pest are not the Drosophilae (2-4 mm long), but the colourful Tephritidae family (&amp;gt; 5000 species). These attack mango fruits throughout the season and lay their eggs right under the (affected) skin of mature green or ripe fruits. They have three generations and multiply very rapidly. Within one or two days the eggs hatch into white maggots. The maggots feed on the fruit, which starts to rot. After 4 to 17 days, the maggots make holes in the skin and leave the fruit to pupate. &lt;br /&gt;
&lt;br /&gt;
The main fruit fly pests of mango in Africa are Ceratitis capitata (Medfly), C. fasciventris, C. rosa and C. cosyra (the most destructive). C. cosyra is however rapidly displaced by Bactrocera invadens (endemic to Sri Lanka)[http://www.ncbi.nlm.nih.gov/pubmed/19610411][http://www.ncbi.nlm.nih.gov/pubmed/22251685], being predominantly a low-land pest.[http://www.ncbi.nlm.nih.gov/pubmed/16923206] B. invadens populations quickly increase with the onset of the raining season[http://www.ncbi.nlm.nih.gov/pubmed/19449630] and also lay aggs in young fruits. Fruit fly populations peak in the late dry season.[http://www.ncbi.nlm.nih.gov/pubmed/17598527]&lt;br /&gt;
&lt;br /&gt;
[u]Control[/u]: Collect all the fallen and affected fruits and bury them deep (&amp;gt;50 cm) into the soil. Or you can use them to breed maggots in a sealed room for fish feed. Natural enemies of tephritids include Diapriidae (tiny wasps; &amp;lt;8mm), Braconidae (also parasitoid wasps) and Oecophylla longinoda (Latreille; a weaver ant). &lt;br /&gt;
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C. cosyra, but particularly C. fasciventris and Medfly may be effectively controlled by using the host-marking pheromone.[http://www.ncbi.nlm.nih.gov/pubmed/23356072] B. invadens [http://www.ncbi.nlm.nih.gov/pubmed/24293246], the Mediterranean fruit fly (C. capitata) and the Mexican fruit fly (Anastrepha ludens) may be effectively controlled by the &amp;#039;sterile insect technique&amp;#039; (releasing overwhelming numbers of sterile insects). The effects of sterile flies are greater than those for parasitoid wasps, but they are complementary.[http://www.ncbi.nlm.nih.gov/pubmed/15568340] &lt;br /&gt;
Medfly eggs and instars (right under the peel) may be killed by immersing Ataulfo mangoes for 95 min in warm water at 47°C, also positively modifying pH (producing more palatable fruits), but can also produce a loss of firmness and weight (5%).[http://www.ncbi.nlm.nih.gov/pubmed/23356057] B. invadens is no more heat tolerant than Medfly.[http://www.ncbi.nlm.nih.gov/pubmed/21404834] &lt;br /&gt;
&lt;br /&gt;
[u]Bait traps[/u]; &lt;br /&gt;
Pheromone traps (85% methyl-eugenol, 5% Naled (insecticide), 10% saturated sugar) can be used for trapping male flies (Jackson trap). A three-component attractant (ammonium acetate, putrescine, and trimethylamine lures) may be used to trap female population of Medfly. (No wind: 28 m. range).[http://www.ncbi.nlm.nih.gov/pubmed/21061993] Malathion-bait sprays and Phloxine B (a xanthene dye; D&amp;amp;C Red #28) are equally effective.[http://www.ncbi.nlm.nih.gov/pubmed/11777044]&lt;br /&gt;
Of six commercial food-based attractants, Nulure captured the greatest proportion of females: 74% compared with 51-68%. But Mazoferm E802 (with or without Spinosad) and Torula yeast captured 2.4-2.6 times more females and 3.4-4.0 times more males than Nulure (also more effective than GF-120, Hymlure and Biolure).[http://www.ncbi.nlm.nih.gov/pubmed/24665714]&lt;br /&gt;
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[u]Fopius arisanus (Sonan)[/u]; F. arisanus is a tiny but most effective[http://www.ncbi.nlm.nih.gov/pubmed/12241567] and the most dominant parasitoid wasp. As parasitism increases, fruit fly infestation decreases.[http://www.ncbi.nlm.nih.gov/pubmed/17598524] Of 3 commonly used bait sprays, which all suppress fruit fly populations, Spinosad and Phloxine B bait sprays are less harmful to these wasps than Malathion bait spray. [http://www.ncbi.nlm.nih.gov/pubmed/11561838]   &lt;br /&gt;
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[u]Diachasmimorpha longicaudata[/u]; D. longicaudata (longtailed fruit fly wasp) is the most important parasitoid wasp used as part of integrated pest management programs against fruit flies such as B. invadens and Ceratitis species. They lay one or more (when hosts are scarse) eggs in fruit fly larva.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014816/] Introduction of D. longicaudata poses minimal competitive risk to F. arisanus.[http://www.ncbi.nlm.nih.gov/pubmed/12241567]&lt;br /&gt;
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[u]Oecophylla longinoda[/u]; O. longinoda is a predator weaver ant that may eat whatever insect or bug they may catch, including caterpillars, beatles, honey bees, driver ants (Dorylus nigricans Ill.) and larvae. O. longinoda is a natural enemy of fruit flies as it eats their larvae. This weaver ant is fiercely territorial and will keep other species of ants (like aphids-farming black ants) at bay. The ants continuously patrol the tree that they live in and may also catch egg-laying female fruit flies in the act, and eat them. B. invadens and C. spp numbers on Kent mangoes may be greatly reduced by Oecophylla longinoda.[http://www.ncbi.nlm.nih.gov/pubmed/17598527] &lt;br /&gt;
Weaver ants, however, also feed on honeydew. They may farm a wide range of honeydew-producing Homoptera (bugs with sucking mouthparts, including scales, aphids and mealybugs), but are mostly associated with Coccids (Saissetia; no virus vector). Only when other Coccids are scarce or unavailable, O. longinoda may farm aphids and mealybugs (unarmoured scale insects that are virus vectors). Mainly in small trees and shrubs, the mechanical damage caused by Coccids is related to the size of the attendant ant colony. Dense populations of O. longinoda are supported by interplanting with clove.[http://www.cabdirect.org/abstracts/19540500227.html;jsessionid=36A454B839F0DEEADFA83F99A7206E6D]&lt;br /&gt;
For the protection of their harvested honeydew, these weaver ants weave a translucent silken tent around living leaves. They use the silk produced by their larvae. It is claimed that pheromones produced by the weaver ants repel egglaying fruit flies. A study in Senegal, however, found that pheromones produced by O. longinoda did hardly repel egglaying females of B. invadens.[http://www.ddrn.dk/filer/forum/File/Abstract_Christina_Abel.pdf]&lt;br /&gt;
On the other hand, trees colonized by O. longinoda (in Ghana) had only 6 to 10% fly infestation, compared to 3% fly infestation in trees treated with Cypermethrin plus Dimethoate. (1614 mg per tree)[http://ugspace.ug.edu.gh/handle/123456789/2387] &lt;br /&gt;
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[u]Praying mantids[/u] may also catch and eat fruit flies. Praying mantids are opportunist predators and do not farm any insects or other bugs. Particularly young mantids may not yet be too large to prey on fruit flies (tephredids). The younger and/or smaller the praying mantids are, the smaller the insects (and any other living creature they may catch) they prey on. Praying mantid nymphs initially feed on aphids and fruit flies (drosophila), and as they grow, they skip to larger preys, such as tephritids, and eventually moths and even much bigger preys.&lt;br /&gt;
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[u]Chemicals[/u]; If you have no other option, GF-120 (Dow chemical) may greatly (81-89% after 7-10 weeks) reduce the number of B. invadens pupae per kg mango.[http://www.ncbi.nlm.nih.gov/pubmed/19449630] Or use Dioptries 80% at the rate of 1 L in 450 L water or Malathion 57% at the rate of 0.5 L to 450 L water / acre. Broad-spectrum insecticides are most damaging to fruit flies&amp;#039; natural enemies such as parasitoid wasps.[http://www.ncbi.nlm.nih.gov/pubmed/19886446] &lt;br /&gt;
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==Long-horn Beetles==&lt;br /&gt;
[[Image:Stem_borer.jpg|thumb|left| Stem Borer [http://www.bitterrootrestoration.com/mango/stem-borer-batocera-rufomaculata.html ©]]]&lt;br /&gt;
[[Image:Dastarcus_helophoroides.jpg|thumb|right| D. helophoroides[https://www.ffpri.affrc.go.jp/labs/seibut/bcg/bcg00284.html ©]]]&lt;br /&gt;
[[Image:Batocera_rufomaculata_2.jpg|thumb|left| Batocera rufomaculata [http://agritech.tnau.ac.in/crop_protection/crop_prot_crop_insectpest%20_Mango_pest&amp;amp;disease.html © TNAU]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Batocera_rubus.jpg|thumb|left| Batocera rubus [http://163.20.112.34/~afu/77v.htm © ]]]&lt;br /&gt;
[[Image:Batocera_baby.jpg|thumb|left| baby Batocera [https://www.flickr.com/photos/bondingtool/14291758294 © Samantha - thebondingtool.com]]]&lt;br /&gt;
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* [u]The (red-spotted) Stem Borer (Batocera rufomaculata)[/u], is a large long-horned beetle (up to 5 cm long body). It cuts incisions on the bark and lays its eggs (up to 200) inside the cuts or cracks of the (damaged) tree bark, trunk or cavities (eg exposed roots). The beetles feed on the bark of living trees and eat the green of young shoots. The grub of the stem borer pupates and feeds inside the stem of mango trees (and other fruit trees, including durian, figs, avocado, jackfruit and cashew nuts), creating a tunnel towards the surface (up to 2 cm in diameter), eventually becoming a beetle. Sap (from the tree) and frass (or coarse sawdust, from the larvae) may drip from the holes. Older larvae may also tunnel deeper in the trunk or branches. This causes drying of branches, and the tree may die. The incidence of infestation of trunk borer is highly influenced by maximum temperature. Infestation is highest when temperature and humidity are high.[http://www.ncbi.nlm.nih.gov/pubmed/24498801] For reproduction, it needs to lay its eggs in a stem that is at least 7 cm in diameter. The Stem Borer has been observed in the west coast of Africa, Madagascar, Mauritius, Réunion, Seychelles, Turkey, Israel, Syria, Iraq, Iran, Pakistan, India, Bangladesh, Sri Lanka, China, Nepal, Thailand, Indonesia, Malaysia and the West Indies. Adults may live up to 4 months. Full grown larva may be 8 to 10 cm long, and may live up to a year, causing a lot of damage. Larval and prepupal + pupal stage lasts about 280 and 24-29 days.[http://www.actahort.org/books/787/787_41.htm]&lt;br /&gt;
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* [u]The (yellow-spotted) Mango Longhorned Beetle (Batocera rubus)[/u] is also a wood borer and about equally large as the Stem Borer. Full grown larvae are 6 to 8 cm long. It attacks various fruit trees, including mango, breadfruit and figs, but also bonsay trees, rubber trees and freshly felled timber. Batocera rubus has been observed in Pakistan, India, Bangladesh, China, Cambodia, Indonesia, Malaysia, Phillipines, Thailand, Taiwan and Vietnam.&lt;br /&gt;
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[u]Natural enemies[/u]; Tiny parasitoid wasps such as Oobius agrili sp.n. (1 mm long), Spathius agrili, Avetianella batocera, Tetrastichus planipennisi and Avetianella xystrocerae sp.n (1.5 mm long) have been shown effective against (the larvae of) some specific wood-boring beetles (Agrilus planipennis, Agrilus sexsignatus and Xystrocera globosa)[http://www.emeraldashborer.info/files/Zhang%20et%20al%202005%20Oobius_Leah.pdf][http://www.fs.fed.us/nrs/pubs/other/2013/2013_McManus_FHTET-13-01.pdf]. Batocera rufus (the mango longhorn beetle), is parasitized (its eggs) by  Louricia ovivora, gen. et sp. n.(a moth), and Ooencyrtus batocerae, sp. n.(a wasp) [http://cabdirect.org/abstracts/19360501471.html;jsessionid=A9E2E64A372836E8E24F2B4E97287DC9] Oobius batocera is an opportunistic parasytic wasp that parasitizes various insect eggs, including beetle eggs. [http://www.nhm.ac.uk/research-curation/research/projects/chalcidoids/database/synonyms.dsml?FamilyCode=EE&amp;amp;ValFamTrib=&amp;amp;VALGENUS=Oobius&amp;amp;VALSPECIES=batocerae&amp;amp;VALAUTHOR=(Ferri%E8re)&amp;amp;VALDATE=1936&amp;amp;ValidAuthBracket=false&amp;amp;HOMCODE=0&amp;amp;&amp;amp;listPageURL=listChalcids.dsml%3FSuperfamily%3DChalcidoidea%26Family%3DEncyrtidae%26Genus%3DOobius] &lt;br /&gt;
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There are also various mites that parasitize Batocera longhorned beetles.[http://www.jstor.org/discover/10.2307/3225250?uid=3738736&amp;amp;uid=2&amp;amp;uid=4&amp;amp;sid=21103843600301] Tetrapolipus diastocerae n. sp., Tetrapolipus afrobatocerae n. sp., Tetrapolipus ramarajui n. sp. and Tetrapolipus seemani n. sp. are described. Tetrapolipus afrobatocerae has been observed in Africa, Tetrapolipus hunteri in Australia.[http://www.researchgate.net/publication/233099056_The_Genus_Tetrapolipus_(Acari_Podapolipidae)_Parasites_of_Tropical_and_Semitropical_Cerambycidae_(Coleoptera)_New_Distribution_Records_and_Descriptions_of_Four_New_Species] Tetrapolipus sulawesiensis is a mite that parasitizes Batocera herculus.[http://d.wanfangdata.com.cn/periodical_dwfl200202010.aspx] &lt;br /&gt;
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[u]Dastarcus helophoroides[/u]; This predator/parasitoid beetle (originally from China and Japan) is an important natural enemy of longhorned beetles. In the larval stage they attack various long horn beetles, including Batocera horsfieldi and Anoplophora glabripennis. Populations are attracted specifically by the frass of their original host. Eggs are laid twice a year, near the host entrance hole, frass-extrusion holes or larval tunnel walls, guided by semiochemicals. The freshly hatched (at about 21°C) first instars (0.7 mm in length) have legs, and actively move to the host. They bite and paralyze the host, and then suck hemolymph and degenerated tissues from the dead or paralyzed prey. Adults can live over 4 years. [http://link.springer.com/article/10.1007%2Fs10526-009-9224-y#page-1] Mass production is feasible if hatched larvae are fed cerambycid larvae until they are about 8 mm in lentgh, and subsequently reared on artificial diet (silkworm pupa powder, dry yeasts, yeast extract, sucrose, peptone, squid oiver oil, preservatives and distilled water). The full grown larvae are about 18 mm in length.[http://link.springer.com/article/10.1023%2FA%3A1009936609401#page-1] Adults don&amp;#039;t readily disperse; they need to be released on each tree separately. And even if you release them at 1.2 m height, their effectiveness decreases with increasing height in the tree. Yet their effectiveness may be very high; up to 85% host mortality. [https://www.ffpri.affrc.go.jp/labs/kanko/401-1.pdf]&lt;br /&gt;
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[u]Control[/u]; The stem borer is active during the night and attracted by light, so that light traps may be effective. Bacillus thuringiensis is a bacterium commonly used as a biological pesticide, and naturally present in the gut of various catterpillars. This bacterium is toxic to many insects. A specific strain of this bacterium, Bacillus thuringiensis ZQ-89 is also toxic to adult long-horned beetles.[http://www.ncbi.nlm.nih.gov/pubmed/21332894] Prevent the stem borer from laying eggs in the bark by applying a thick layer of coaltar on the stem. Or one may paint (at 0.5%) adult mango trees with monocrotophos or phosalone.[http://www.cabdirect.org/abstracts/19790379463.html] One may also specifically apply the insectisides to the potentially affected sites (cracks and cavities), and to twigs and young shoots to deter feeding by adults. One may poke with a hard wire to physically damage, and/or inject insectisides where larvae are suspected. Chloroform, ethyl acetate, Metasystox [demeton-S-methyl] and a mixture of petroleum and kerosene oil (at 5 ml/bore) and ethylene dibromide (at 3 ml/bore) gave 100% mortality of B. rufomaculata larvae.[http://www.cabdirect.org/abstracts/19881107545.html] Plaster the holes with wet clay, aluminium phosphide tablets (3 g/hole) and dichlorvos (0.1% spray).[http://www.cabdirect.org/abstracts/19790379463.html] Among the chemical treatments, Imidacloprid 17.8% SL, Thiamethoxame 25% WG was found best in management of mango stem borer.[http://www.gifre.org/admin/papers/gjbahs/management-vol-2-4-gjbahs.pdf]&lt;br /&gt;
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==Mango Hoppers==&lt;br /&gt;
[[Image:Idioscopus_niveosparsus.jpg|thumb|left| I. niveosparsus [http://163.20.112.34/~afu/641x.htm ©]]]&lt;br /&gt;
[[Image:Mallada_basalis.jpg|thumb|right| Mallada sp. [http://3insect.blogspot.nl/2012/06/mallada-basalis.html © Chen KunTsan]]]&lt;br /&gt;
[[Image:Idioscopus_clypealis.jpg|thumb|left| I. clypealis [http://163.20.112.34/~afu/641y.htm ©]]]&lt;br /&gt;
[[Image:Mallada_signata_3.jpg|thumb|right| Mallada signata [http://www.oocities.org/brisbane_lacewings/Chrysopidae.htm © Keith Power]]]&lt;br /&gt;
[[Image:Idioscopus_nitidilus.jpg|thumb|left| I. nitidilus [http://tech.groups.yahoo.com/group/pestnet/message/6414 ©]]]&lt;br /&gt;
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Mango hoppers:&lt;br /&gt;
* Idioscoynio chypeabis&lt;br /&gt;
* Idioscopus niveosparsus&lt;br /&gt;
* Idioscopus clypealis&lt;br /&gt;
* Idioscopus nitidulus&lt;br /&gt;
* Amritodus atkinsoni aka mango leafhopper&lt;br /&gt;
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Mango hopper nymphs as well as adults puncture and suck the sap of tender parts of the mango plant. Heavy drainage will cause the leaves to curl and dry. Infested flowers will shrivel and turn brown. Hoppers also secrete honeydew, which may cause sooty mould, inhibiting the leaves from obtaining energy from daylight. Sufficient spacing of trees and pruning of overlapping branches are essential in preventing hopper infestation, as shade (and high humidity) favour hopper multiplication.&lt;br /&gt;
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[u]Natural enemies[/u]:&lt;br /&gt;
Parasites: Aprostocetus sp., Gonatocerus sp. and Polynema sp. parasitize eggs of Idioscopus clypealis and Idioscopus nitidulus.[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
A preparation of the fungus Beauveria bassiana is also somewhat effective against mango hoppers. Chrysopa lacciperda [Plesiochrysa lacciperda] and Mallada boninensis predate on on nymphs of I. clypealis, I. nitidulus and Amritodus atkinsoni.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9]&lt;br /&gt;
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[u]Mallada boninensis[/u]; This green lacewing is an effective [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=5&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFQQFjAE&amp;amp;url=http%3A%2F%2Fwww.researchjournal.co.in%2Fonline%2FIJAS%2FIJAS%25203(2)%2F3_A-164-166.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNGZ9IidKvjO6s1zNs0HvbUMpKKPBg] generalist predator, feeding on mealy bugs (Mani and Krishnamoorthi, 1987), white flies (Selvakumaran et aI., 1996), bollworms and aphids (Kabissa et al., 1996) and on nymphs of Aphis gossypii, Aphis craccivora and Rhopalosiphum maidi [http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=3&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEAQFjAC&amp;amp;url=http%3A%2F%2Fwww.ets-staffing.com%2Fcurrentbiotica%2Fjournals6-issueiii%2Fcb-6-3-short-notes-3.pdf&amp;amp;ei=laVvU-Evg9E589aBEA&amp;amp;usg=AFQjCNETNWfEOrZznoJjj0ZrFFOINJ2liw], blackflies, psylla and leaf miner. Green lacewings are recommended for the integrated pest management programme (Nehare et al., 2004). M. boninensis feeds well on Corcyra cephalonica eggs[http://cabdirect.org/abstracts/20113350526.html;jsessionid=69EA3BE15508EF9C029CE688C342FD4D] (laboratory host) C. cephalonica can be mass reared on Jowar along with groundnut, streptomycin, vitamin complex, Na and K salts.&lt;br /&gt;
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[u]Control[/u]: &lt;br /&gt;
Spray Imidacloprid (0.005%; 0.3 ml/L water), acephate 75SP (1.5 g/L water), Etofenprox (0.03%), Phosalone (1.5 ml/L water), Carbaryl (0.15%; 3 g/L water), Monocrotophos (0.04%), Phosphamidon (0.05%) or Methyl Parathion (0.05%). &lt;br /&gt;
* Spray 3 times; First spray should be given during the early stage of flower formation. The second spray should be given at a flower size of 6 to 8 cm, still before blooming. The third spray should be given after fruit setting, when the fruits are the size of a pea. Or:&lt;br /&gt;
* First spray should be given during the early stage of flower formation. The second spray should be given 2 weeks later. Or:&lt;br /&gt;
* Spray 3 times; First spray of imidacloprid (0.005%; 0.3 ml/L water) should be given during the early stage of flower formation. The second spray, thiamethoxam (0.005%; 0.2 g./L water) or acephate 75SP (1.5 g/L water) should be given at after fruit setting. If hopper infestation persists, the third spray, of carbaryl (0.15%; 3 g/L water) should be given prior to fruit maturation.&lt;br /&gt;
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Imidacloprid residues persist in peel for 60 days and in pulp for 50 days. Mature Dashehari fruits at harvest (after 85 days of spraying) were free from imidacloprid residues.[http://www.ncbi.nlm.nih.gov/pubmed/23196371]&lt;br /&gt;
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==Mango Midges==&lt;br /&gt;
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[u]Inflorescence Midge[/u]&lt;br /&gt;
[[Image:Platygaster_sp.jpg|thumb|right| Platygaster sp. [http://liuzhen.000space.com/insect/organism.php?id=1367&amp;amp;type=list]]]&lt;br /&gt;
[[Image:Inflorescence_midge.jpg|thumb|left| [http://www.bitterrootrestoration.com/mango/inflorescence-midge-erosomyia-indica.html Erosomyia indica ©]]]&lt;br /&gt;
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This midge (Erosomyia indica) lays eggs in new inflorescence (preventing flower and fruit set) and new leaves around it, and then the larvae will eat their way through. The maggots penetrate and feed on the tender parts of the plant, such as shoots, buds and flower buds. The flowers will dry and fall off. For pupation, the mature larvae will drop into the soil. The adult midge lives only one day and doesn&amp;#039;t cause any damage. Maggots from eggs laid on new fruits will eat their way into the fruit, which will turn yellow and drop.[http://www.actahort.org/books/231/231_15.htm] The inflorescence midge is an important pest in India in particular.&lt;br /&gt;
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Infloresence midge is parasitized by Platygaster sp., Eupelmus sp. and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19881115612.html;jsessionid=E4CDBFFBEE75C82EDA1BA2CA7B5F1DD2], which all also parasitize Gall Midges. The inflorescence midge is also parasitized by Aprostocetus sp.[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9] and Mirufens longifunculata. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false]&lt;br /&gt;
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Another mango midge, Procystiphora mangiferae is parasitized by Pirene sp. [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], which also parasitizes mango gall midges.&lt;br /&gt;
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Chemicals: If you have no other option, Parathion, Dimethoate, Phosphamidon and Endosulfan much reduced infestations on mango shoots.[http://www.cabdirect.org/abstracts/19740517480.html;jsessionid=59B14F930CB8EDFD2F1B50F1B04C7692] The most effective insecticide for control appeared to be phosphamidon (Dimecron), whether mixed with diazinon or not, as a foliar spray. [http://www.cabi.org/isc/abstract/19770549886]&lt;br /&gt;
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[u]Gall midges[/u]&lt;br /&gt;
[[Image:Mango_galls.jpg|thumb|left| [http://www.infonet-biovision.org/default/ct/124/crops © A.M. Varela]]]&lt;br /&gt;
[[Image:Eupelmus_urozonus.jpg|thumb|right| Eupelmus urozonus [http://www.biolib.cz/en/image/id150459/ © Miroslav Fiala]]]&lt;br /&gt;
[[Image:Mango_galls_P_matteiana.jpg|thumb|left| P. matteiana galls [http://www.pests.blogfa.com/8907.aspx © RJ Gagné]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Gall_midget_exit_holes.jpg|thumb|left| Exit holes [http://biostor.org/reference/55262 © RJ Gagné]]]&lt;br /&gt;
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There are various mango gall flies:&lt;br /&gt;
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- Erosomyia mangifereae or Procontarinia frugivora has been observed in the Philippines, India, West Indies and Brazil.&lt;br /&gt;
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- Asynapta sp. in West Indies.&lt;br /&gt;
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- Dasyneura mangifereae in Hawaii.&lt;br /&gt;
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- P. allahabadensis, P. biharana, P. brunneigallicola, P. viridigallicola, P. echinogalliperda, P. keshopurensis, P. mangifoliae, P. tenuispatha, P. amraeomyia and Dasyneura amaramanjarae have been observed in India (and some in Pakistan).&lt;br /&gt;
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- P. mangicola in China and Japan.&lt;br /&gt;
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- P. matteiana (1.5 mm long) in India, Kenya, Mauritius, Java and Réunion[http://biostor.org/reference/55262][http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=4&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CFEQFjAD&amp;amp;url=http%3A%2F%2Fwww.geocities.ws%2Fmdce2005%2FMDCE2005%2F27-98_102.pdf&amp;amp;ei=HS5zU-P3DomXyQOd_IHgCA&amp;amp;usg=AFQjCNHlZtUS8qYFE2lCVSqh3ow0K4ukLg&amp;amp;sig2=vW-1UFdjzeE3l58kmc8eMg&amp;amp;bvm=bv.66699033,d.bGQ]. &lt;br /&gt;
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The tiny (1-2 mm) mango gall flies lay their eggs on the surface of young leaves, buds, shoots and flowers. After hatching, the maggots will eat their way into the plant tissue. This will show as galls (3-4mm) on the leaves. Once mature, they drop to the ground to pupate. The holes that they left behind are susceptible to fungal infections. Infested fruits initially show small (1mm) brownish lesions, which grow as the fruit grows. In young fruits, the exit holes are usually near the point of attachment, on the bottom side. Most infested fruits fall to the ground before ripening. Optimal conditions for reproduction is 26°C and a relative humidity of 70 to 80%. Infestation particularly occurs at bud-burst stage, at fruit set and on tender leaves of new flushes.[http://openagricola.nal.usda.gov/Record/IND92003672]&lt;br /&gt;
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[u]Natural enemies[/u]; Parasitic insects such as Platygaster sp., Eupelmus sp., Tetrastichus sp., Systasis dasyneurae [http://www.cabdirect.org/abstracts/19881115612.html], Pirene sp. and the predator ants Formica rufa (aka red wood ant, native to Europe and North America), Oecophylla longinoda (weaver ant native to West and Central Africa) and Oecophylla smaragdina (native to South-east Asia) and Camponotus sp. (aka carpenter ant, native to forests world wide).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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[u]Baits[/u]; Coriander oil attracts Erosomyia. D. amaramanjarae is attracted by a mixture of glycine, sodium hydroxide, ammonium carbonate and strong ammonia solution. Bordeaux mixture is a repellent. &lt;br /&gt;
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[u]Chemicals[/u]; Various systemic insecticides are equally effective: Dimecron (phosphamidon), Anthio (formothion) and Metasystox (demeton-S-methyl). Most effective is a mixture of phosphamidon (0.03%) and diazinon (0.03%).[http://www.cabdirect.org/abstracts/19881103898.html]&lt;br /&gt;
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==Mango Moths==&lt;br /&gt;
[[Image:Indarbela_quadrinotata.jpg|thumb|left| Indarbela quadrinotata [http://www.agriplaza.in/orange-protection.htm#indarbela © Agriplaza]]]&lt;br /&gt;
[[Image:Podagrionella_habitus.jpg|thumb|right| Podagrionella [http://www.waspweb.org/Chalcidoidea/Torymidae/Toryminae/Podagrionini/Podagrionella/index.htm © S. van Noort]]]&lt;br /&gt;
[[Image:Indarbela_quadrinotata_2.jpg|thumb|left| Indarbela quadrinotata [http://www.ku.ac.th/e-magazine/september43/bark_feeders/ © KUAC]]]&lt;br /&gt;
[[Image:Mantis_religiosa_5.jpg|thumb|right| Mantid eating large moth [http://www.agefotostock.com/en/Stock-Images/Rights-Managed/K60-589612 © Oriol Cabrero]]]&lt;br /&gt;
[[Image:Eudocima_fullonia.jpg|thumb|left| Eudocima fullonia [http://www.iwaidja.org/site/animals/ © Iwaidja Inyman]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Eudocima_homaena_2.jpg|thumb|left| Eudocima homaena [http://gaga.biodiv.tw/9702bx/049.htm © Gaga]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Eudocima_homaena.jpg|thumb|left| Eudocima homaena [https://www.flickr.com/photos/suedechen/8063437610/ © Suede Chen]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-l5.php © PathPiva]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Cryptoblabes_gnidiella_2.jpg|thumb|left| Cryptoblabes gnidiella [http://pathpiva.wifeo.com/cryptoblabes-gnidiella-1.php © PathPiva]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Orthaga_exvinacea.jpg|thumb|left| Orthaga exvinacea [http://www.nbaii.res.in/insectpests/images/Orthaga-exvinacea7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Orthaga_euadrusalis.jpg|thumb|left| Orthaga euadrusalis [http://www.jpmoth.org/~dmoth/62_Pyralidae/6003_Epipaschiinae/60031001_Orthaga_euadrusalis_1865/Orthaga%20eudrusalis%200208287801.jpg]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_2.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42845]]]&lt;br /&gt;
[[Image:Aleiodes_indiscretus.jpg|thumb|right| Aleiodes indiscretus [http://commons.wikimedia.org/wiki/File:Aleiodes_indiscretus_wasp_parasitizing_gypsy_moth_caterpillar.jpg © Agricultural Research Service]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera.jpg|thumb|left| Citripestis eutraphera [http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42848]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Citripestis_eutraphera_3.jpg|thumb|left| Citripestis eutraphera [http://www.boldsystems.org/index.php/Taxbrowser_Taxonpage?taxid=375866 © ANIC/BIO]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_2.jpg|thumb|left| P. jocosatrix [http://www.nbaii.res.in/insectpests/images/Penicillaria-jocosatrix7.jpg © NBAII]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix_3.jpg|thumb|left| P. jocosatrix [http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar © DAFF]]]&lt;br /&gt;
[[Image:Euplectrus_sp.jpg|thumb|right| Euplectrus sp [http://www.pbase.com/tmurray74/chalcid_wasps_eulophidae © Tom Murray]]]&lt;br /&gt;
[[Image:Penicillaria_jocosatrix.jpg|thumb|left| P. jocosatrix [http://www.flickr.com/photos/bettaman/3973171117/ ©]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Caterpillar.jpg|thumb|left| Deanolis sublimbalis [http://www.padil.gov.au/pests-and-diseases/pest/main/136263/43328 © S. Eyres]]]&lt;br /&gt;
[[Image:Deanolis_sublimbalis_Moth.jpg|thumb|left| Deanolis sublimbalis [http://www.ces.csiro.au/aicn/name_s/b_1295.htm © DAFF]]]&lt;br /&gt;
[[Image:Trichogramma.jpg|thumb|right| Trichogramma sp. [http://agritech.tnau.ac.in/farm_enterprises/Farm%20enterprises_%20bio%20pesticides.html © DAFF]]]&lt;br /&gt;
[[Image:Chlumetia_transversa_2.jpg|thumb|left| C. transversa [http://www.nbaii.res.in/insectpests/Chlumetia-transversa.php © NBAII]]]&lt;br /&gt;
[[Image:Chlumetia_transversa.jpg|thumb|left| C. transversa [http://www.inaturalist.org/observations/572035 © T Bonebrake]]]&lt;br /&gt;
[[Image:Goryphus_basilaris.jpg|thumb|right| Goryphus basilaris [http://www.hkwildlife.net/viewthread.php?tid=49119 © Daydream]]]&lt;br /&gt;
[[Image:Parasa_lepida.jpg|thumb|left| P. lepida [http://fanseab.exblog.jp/9707580]]]&lt;br /&gt;
[[Image:Agriosphodrus_dohrni.jpg|thumb|right| Agriosphodrus dohrni [http://www.melodymcfarland.com/?paged=25 © Melody]]]&lt;br /&gt;
[[Image:Parasa_lepida_2.jpg|thumb|left| P. lepida [http://www.isan.clubs.chula.ac.th/para_norkhai/?transaction=post_view.php&amp;amp;cat_main=all&amp;amp;id_main=301&amp;amp;star=270&amp;amp;pg=28 © ปิ่นลม]]]&lt;br /&gt;
[[Image:Parus_major.jpg|thumb|right| Parus major [http://en.wikipedia.org/wiki/Parus_major#mediaviewer/File:Parus_major_2_Luc_Viatour.jpg © Luc Viatour / www.Lucnix.be]]]&lt;br /&gt;
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[u]Bark Eating Caterpillar[/u]&lt;br /&gt;
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The Bark Eating Caterpillar (Indarbela quadrinotata and Indarbela tetraonis) aka Mango Bark Feeder has been observed in India, Bangladesh and Sri Lanka. The moth has a wingspan of maximally 2 cm. This wood boring insect may attack a wide variety of trees, including mango, guava, jujube, pomegranate and apple trees. In plantations, infestation generally starts with the onset of premonsoon rains. The main symptom is plastered dark-brown masses on tree trunks and large branches. These webbed masses consist of chewed on wood remainders / frass, excrements and silken thread. Eggs are laid in clusters on the bark of trees. Initially, the newly hatched larvae occur in groups browsing on the bark. Then they migrate and lodge in shoots and buds. As the larvae grow, they start tunneling into the wood, up to 25 cm deep. This tunnel is kept free of frass. The frass and excreta from the tunnels are used for constructing the extended tunnel mouth (sleeve). As the larvae get bigger, they make the tunnel wider. The larvae hide in these tunnels during the day and come out at night to eat the surrounding bark of the tree. The rate of feeding is faster during the hottest months. The larva minimally eats 16 mg / day, at 12.5 degrees Celsius. The maximum is 166 mg/day at 35 degrees Celsius. Similarly, food consumption is maximum at 96% RH and minimum at 56% RH.&lt;br /&gt;
When large patches of bark are eaten away, the tree gets increasingly exposed to various bacteria, fungi etc. And if substantial areas of bark have been eaten, the tree will eventually die. Prolonged water stress decreases the resistance of the host tree. The larvae pupate inside the tunnel and the emerging moth leaves its &amp;quot;pupal skin&amp;quot; at the mouth of the borer hole. The pupa is 1.5 cm long. The larval stage lasts for about 8 months. The pupal period lasts for about 9 days. It has a little more than one generation per year.  [http://www.tropecol.com/pdf/open/PDF_49_1/09%20Shashidharan.pdf]&lt;br /&gt;
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Natural enemies; Two fungi; Aspergillus candidus and Beauveria bassiana have been reported to cause mortality of the Bark Eating Caterpillar in the field. Laboratory trials have indicated 100% larval mortality by these fungi. The Bark Eating Caterpillar is parasitized by Podagrionella indarbelae (&amp;#039;metallic&amp;#039; parasitic wasps), which may be fed the eggs of I. tetraonis.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf] Podagrionella is found in tropical African countries, Senegal, Algeria, Malawi, France, Spain, India, Thailand, Indonesia, The Philippines, Australia&lt;br /&gt;
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Control; prevent overcrowding by sufficient spacing in between trees. One may insert a sharp metallic probe/spike into each tunnel/hole to kill the larvae. One may seal the tunnel entrance using tar or wax. Of the various insecticides screened against this insect, monocrotophos (0.1 %), quinalphos (0.1 %) and fenvalerate (0.08%) gave best results.[http://docs.kfri.res.in/KFRI-RR/KFRI-RR122.pdf]&lt;br /&gt;
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[u]Fruit-piercing Moths[/u] Eudocima (aka Othreis) are very large moths (up to 10 cm wingspan) with orange abdomen. The most widespread specie is Eudocima fullonia (aka Eudocima phalonia)[http://www.hindawi.com/journals/tswj/2011/753484/], one of the most damaging in many tropical lowland regions from West Africa eastwards to the Pacific [http://www.thaiscience.info/journals/Article/Biological%20and%20taxonomic%20studies%20on%20immature%20and%20adult%20fruit-piercing%20moths%20in%20nepal,%20with%20reference.pdf], also predominant in India.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41] Eudocima moths look like dead leaves when in hiding mode. Until they show their bright orange hindwings with broad black margins and a large black spot in the middle. Mature larvae are 4 to 5 cm long. These moths have been observed in virtually any tropical country, except for the Americas. The adults fly (very well), feed and mate during the night. In tropical countries, they live up to a month. The adults feed on fruit juice by puncturing the fruit. The resulting wound is used by various bugs and diseases to attack the fruit (including mango, banana, grapes, orange, clementine, kiwi, lychee, pineapple, peach, apricot, papaya apple and pear). Eggs are laid singly, or in masses (up to 750 in a cluster, which may be used for rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings]), on any part of the plant. In tropical conditions, eggs hatch within 4 days. Larvae may feed around the clock and mature in 3 weeks, after which they pupate in cocoons of silk-spun leaves. Pupation favors wet conditions and occurs in 12 to 18 days.[http://www.aphis.usda.gov/plant_health/plant_pest_info/pest_detection/downloads/pra/efulloniapra.pdf] Pupation is always in a roomy cell made of living leaves woven together with silk and slightly lined; the pupa is attached to this lining by the cremaster.[http://www.mothsofborneo.com/part-15-16/calpini/calpini_4.php] Temperatures below 16°C during the activity period after dusk prevents feeding, mating and oviposition.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] Eudocima fullonia was most destructive in Nigeria and Sierra Leone.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41]&lt;br /&gt;
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Their larvae do not feed on mangoes, nor the mango trees. Instead, the larvae feed on very specific Fabaceae and Menispermaceae. Variation in the alkaloids associated with certain menisperm genera (eg quaternary alkaloids in Tinospora[https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-2007-969933]) may explain the very specific (or even exclusive) moth–host plant relationships.[http://www.publish.csiro.au/?paper=SB9960227] Eudocima larvae are known to feed extensively on Stephania japonica (Menispermaceae) and its varieties.[http://www.calodema.com/freefiles/412.pdf] In Australia the larvae of Eudocima salaminia feed almost exclusively on the forest vine, Stephania japonica.[http://journals.cambridge.org/action/displayAbstract?fromPage=online&amp;amp;aid=2631700&amp;amp;fileId=S0007485300033563] Eudocima materna feeds exclusively on Tinospora cordifolia [https://books.google.nl/books?id=UxVFMwEACAAJ&amp;amp;dq=inauthor:%22BHUMANNAVAR+BASAVARAJ+S%22&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=MHaIVL2mBMLwUvDcg5gF&amp;amp;ved=0CCIQ6AEwAA] (widespread in tropical regions [http://link.springer.com/article/10.1007/s00606-004-0293-1#page-1], including Africa[http://informahealthcare.com/doi/abs/10.1080/14756360802333075]).Eudocima homaena may feed on Quisqualis indica.[http://www.aapmhe.in/index.php/pmhe/article/download/42/41], Achyranthes, Cocculus, Cyclea, Menispermum and Tiliacora species.[http://www.en.inforapid.org/index/index.php?search=Cocculus] Eudocima jordani and Eudocima fullonia may be generalist feeders.[http://www.researchgate.net/publication/249439696_Differential_habitat_affinities_of_five_species_of_fruitpiercing_moths_(Lepidoptera_Noctuidae)_in_their_utilization_of_Tinospora_smilacina_Benth._as_a_larval_host_plant_in_north_Queensland]&lt;br /&gt;
Eudocima fullonia larva feed on Erythrina variegata (Indian coral tree in Eastern Africa) and Stephania japonica [http://www.nhm.ac.uk/resources/research-curation/projects/chalcidoids/pdf_x/sandsli2005.pdf], but growth is better and mortaility much lower when feeding on Sarcopetalum harveyanum and particularly Tinospora smilacina (Menispermaceae - the moonseed family), in comparison to Stephania japonica. Tiliacora are also readily accepted by Eudocima fullonia larva.[http://onlinelibrary.wiley.com/doi/10.1111/j.1570-7458.1994.tb01803.x/abstract] Eudocima fullonia larva feed on leaves of Erythrina and vines in the Menispermaceae [http://www.cabdirect.org/abstracts/20066600377.html], including Tinospora homosepala [http://www.wptrc.org/userfiles/file/Physiol%20Entomol-2005.pdf], Tinospora sinensis [http://www.ccs-hk.org/DM/butterfly/Noctuid/Eudocima-phalonia.html], Tinospora cordifolia (in India, Myanmar and Sri Lanka) [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=23012], Tinospora baenzigeri, Tinospora crispa, Tiliacora triandra  (in Thailand).[http://www.thaiscience.info/journals/Article/Biological%20and%20taxonomic%20studies%20on%20immature%20and%20adult%20fruit-piercing%20moths%20in%20nepal,%20with%20reference.pdf] Cocculus hirsutus [http://www.actahort.org/members/showpdf?booknrarnr=890_81], Carronia multisepala, Hypserpa decumbens (in Australia) [http://onlinelibrary.wiley.com/doi/10.1111/j.1570-7458.1994.tb01803.x/abstract] and Legnephora moorei (Australia) [http://onlinelibrary.wiley.com/doi/10.1111/j.1442-9993.1993.tb00471.x/abstract]&lt;br /&gt;
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Tinospora smilacina (aka Snake vine, Tinospora walcottii, Tinospora berneyi) is a tropical herbaceous vine endemic to Australia [http://keys.trin.org.au/key-server/data/0e0f0504-0103-430d-8004-060d07080d04/media/Html/taxon/Tinospora_smilacina.htm], found along the boundaries of dry rainforest and dry eucalyptus forest.[http://www.somemagneticislandplants.com.au/index.php/plants/600-tinospora-smilacina] In Ghana, the following species occur: Tiliacora leonensis [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=120], Tiliacora funifera [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=80], Tiliacora dinklagei [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=140], Tiliacora louisii [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=240] and Tiliacora dielsiana [http://www.gbif.org/occurrence/search?taxon_key=7268630&amp;amp;HAS_COORDINATE=true&amp;amp;HAS_GEOSPATIAL_ISSUE=false&amp;amp;offset=260]&lt;br /&gt;
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Control; Regularly check the orchard during the night using a flashlight. The adult Eudocima moths have large eyes that glow red when illuminated. Adult [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids] may eat large moths such as Fruit-piercing moths. The female Praying mantis lays a few hundred eggs in a cluster (ootheca) attached to an object like a branch or trunk. Large species of mantids may have a length of up to 12 cm. Their colour is usually adaptedto the environment they naturally live in. They have about 2 generations per year. Young mantids look like adults, but don&amp;#039;t have wings. Adult females often have no wings either, or reduced wings.&lt;br /&gt;
Eudocima fullonia is parasitized by Euplectrus maternus [http://link.springer.com/article/10.1023/B:BICO.0000036439.74117.2f#page-1], Euplectrus melanocephalus [http://www.aapmhe.in/index.php/pmhe/article/view/42] and Trichogramma.[http://images.peabody.yale.edu/lepsoc/jls/2010s/2011/2011-65-1-053.pdf]&lt;br /&gt;
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[u]Honeydew Moth[/u]&lt;br /&gt;
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The Honeydew Moth (Cryptoblabes gnidiella aka Albinia casazzar aka Albinia wockiana) aka Christmasberry Moth survives only in warm climates (all over the world). It attacks a large variety of crops, including mangoes, banana, oranges, clementines, grapes, loquats, pomegranates, avocado, coffee, maize and rice. The caterpillars (1 cm long) mainly feed superficially on fruits, but may also feed on leaves, flower, shoots and bark. The newly hatched larvae and the adults, however, solely feed on honeydew. The adults are active during the night. Females mate 1 to 4 times during their entire life. One Honeydew Moth may lay up to 100 eggs within 2 days after mating, on fruit or leaves (averagely 150 in total, during lifetime). These eggs hatch within one week (or longer, in colder regions). It has several generations per year, depending on the climate. Adult moths are good flyers (1 to 2 cm wingspan) and may rapidly spread to poorly maintained orchards (infested with honeydew).&lt;br /&gt;
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Natural enemies; Scolothrips sexmaculatus and Orius spp. prey on the eggs and early-instar larvae. The Honeydew Moth is parasitized by Phanerotoma sp.[http://www.cabdirect.org/abstracts/19740514736.html] and Trichogramma Platneri (both parasitoid wasps).&lt;br /&gt;
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Trichogramma are extremely tiny wasps (&amp;lt; 0.5 mm). They insert their eggs into the eggs of the moth. The larvae feed, grow and pupate inside the host egg, which converts the colour of the host egg from white to black. The emerging wasp eats its way out and is ready to mate. The eggs from unmated females will produce only males, whereas the eggs from mated females will produce both males and females. Adult Trichogramma feed on nectar from flowers. One female wasp may parasitize 50 moth eggs during her life. The optimimum conditions for Trichogramma are 20 to 27 degrees Celsius and 60% RH. Most insecticides kill Trichogramma.&lt;br /&gt;
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Control; This moth is attracted by honeydew secreted by aphids, scale insects and other honeydew secreting bugs. It is therefore most effectively controlled by controlling all honeydew secreting bugs. Its instars are highly susceptible to Bacillus thuringiensis.&lt;br /&gt;
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[u]Leaf Webber[/u]&lt;br /&gt;
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These brown moths (Orthaga euadrusalis and Orthaga exvinacea) are a minor mango (and Cashew nut) pest. They have been observed in India and Indonesia. Females mate only one time during their whole life.[http://www.ncbi.nlm.nih.gov/pubmed/20136014] The leaf webber lays eggs on young leaves and buds and creates a web around pods, leaves and flowers for its larvae to pupate in. Such a webbed cluster may contain several larvae. The Leaf Webber causes relatively very little damage, as the larvae merely scrape and eat from the surface of the leaves.&lt;br /&gt;
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Control: Application of Serratia marcescens (a bacterium), Aspergillus flavus (a fungus) and Beauveria bassiana (a fungus) are all very effective.[http://www.cabdirect.org/abstracts/19810586732.html] The leaf webber is parasitized by Brachymeria lasus, Hormius sp., Pediobius bruchicida and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html] and also by Trichogramma chilonis and Trichogramma pretiosum.[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=37933]&lt;br /&gt;
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[u]Mango Fruit Borer[/u];&lt;br /&gt;
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The Mango fruit borer (Citripestis eutraphera; a moth) has been observed in Indonesia, India and Australia. Its lays its eggs (&amp;lt; 1 mm) on fruit or stalk. The larvae feed on mango pulp (and cashews) and cause premature fruit drop, mainly in young fruit. The larvae hatch in 2 days. They are initially white, then red-violet when young, turning to dark blue as they grow. Hatched larvae initially feed on the peel of the fruit. Then they bore into into the fruit and feed for 12 to 15 days before they pupate in the fallen fruit, or in the soil. Often there is more than one larva in a fruit with a total of 15 recorded in one fruit.[http://www.padil.gov.au/pests-and-diseases/pest/main/142262/42846].&lt;br /&gt;
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Control: Its natural enemies are parasitoid wasps (Aleiodes sp. and Euplectrus sp.) and a parasitoid fly (Blepharella lateralis).&lt;br /&gt;
[http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=9416] To protect the stems, cover them with a cloth or Jute and paste charcoal over it. Fostoxin tablets can also be placed and sealed in the holes made by the borers.&lt;br /&gt;
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[u]Mango Shoot Borer[/u]&lt;br /&gt;
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Mango Shoot Borer (Penicillaria jocosatrix) or Velvet mango slug; P. jocosatrix (wingspan 2 - 2.5 cm) has been observed in Indonesia, The Philippines, Malaysia, Vietnam, Guam, Hawaii, Thailand and Australia. Its larvae; the caterpillar of this moth (up to 3 cm long) causes damage to shoots, stems, inflorescence, flowers and fruits of the mango tree. Pestalotiopsis anacardiacearum sp. nov. (fungal leaf rot) is found on dead mango leaves associated with P. jocosatrix (the Mango Shoot Borer).[http://biotaxa.org/Phytotaxa/article/view/phytotaxa.99.2.1] Eggs hatch in 3-5 days. Larval development takes 8-10 days. Mature larvae pupate in the soil and the moth emerges 16-20 days later.[http://www.daff.qld.gov.au/plants/fruit-and-vegetables/a-z-list-of-horticultural-insect-pests/mango-shoot-caterpillar]&lt;br /&gt;
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Natural enemies: Euplectrus sp. (a parasitoid wasp) and Blepharella lateralis (a parasitoid fly) may effectively decrease Mango shoot borer infestation [http://books.google.nl/books?id=t_BSs0hrAPAC&amp;amp;pg=PA106&amp;amp;lpg=PA106&amp;amp;dq=Erosomyia+indica&amp;amp;source=bl&amp;amp;ots=_Miie9pFAw&amp;amp;sig=21mI-ZT7wX1jm8Y-DVIu9-ALhes&amp;amp;hl=nl&amp;amp;sa=X&amp;amp;ei=zsnFU8mFK8mn0QX9iIGwCg&amp;amp;ved=0CIMBEOgBMAw#v=onepage&amp;amp;q=Erosomyia%20indica&amp;amp;f=false], and are also effective against the Mango fruit borer.&lt;br /&gt;
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[u]Mango Seed Borer[/u]&lt;br /&gt;
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The Mango Seed Borer (Deanolis sublimbalis aka Autocharis albizonalis) is the Red banded mango caterpillar. It is a mango pest in Australia, Burma, The Philippines, Malaysia, Vietnam, China, Indonesia and Papua New Guinea. Severe infestation may cause up to 50% yield reduction. This grey snout moth (wingspan 2 cm) lays its eggs on the top of the mango. Its larvae (one or more per fruit) develop within the fruit in 2 to 3 weeks. Initially they feed on the pulp, and then on the seed. The fruit will split, rot and drop. The mature larvae will pupate in the soil.&lt;br /&gt;
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Natural enemies: Rychium attrisimum are wasps that feed on the larvae of the Mano Seed Borer as they leave the fruit.[http://www.planthealthaustralia.com.au/wp-content/uploads/2013/03/Red-banded-mango-caterpillar-CP.pdf] Trichogramma chilonis and Trichogramma chilotreae are tiny parasitoid wasps that parasitize the eggs of the Mango Seed Borer and hundreds of other moths. They are so small that with the naked eye you cannot see what they are. They have a wingspan of 0.5 mm. They live about 2 weeks, and may lay several eggs in a single moth egg.&lt;br /&gt;
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Chemicals: If you have no other option, Deltamethrin and Cyfluthrin are most effective.&lt;br /&gt;
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[u]Mango Tip Borer[/u]&lt;br /&gt;
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Mango Tip / Shoot / Bark / Trunk / Twig Borer (Chlumetia transversa) aka aka Shoot Boring Caterpillar is a serious mango (and litchi) pest. The major symptom is drying of the tip of young shoots. Female moths lay their eggs on tender leaves. Newly hatched larvae bore into these leaves. As they grow they bore into young shoots near the growing point, and subsequently tunnel down. The leaves of affected shoots will droop. It has four generations each year, and in colder regions overwinters as pupae in young branches and bark. Generations are overlapped.[http://europepmc.org/abstract/CBA/367089] Full grown caterpillars are 2 to 2.5 cm long.&lt;br /&gt;
C. transversa has been observed in Indonesia, The Philippines, Malaysia, Thailand, Taiwan, China, India, Pakistan, Sri Lanka and Australia. &lt;br /&gt;
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The Mango Tip Borer is parasitised by Bracon greeni, Meteorus sp. and Goryphus sp [http://www.cabdirect.org/abstracts/19810586735.html], which are all parasitoid wasps. Its larvae are also parasitized by the fly Megaselia chlumetiae sp. nov. Its larvae enter the host and develop by consuming the host&amp;#039;s internal tissues. Pupariation normally took place within the host&amp;#039;s empty skin.[http://www.cabdirect.org/abstracts/19921163681.html]&lt;br /&gt;
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Control; If you have no other option, spraying 2.5% deltamethrin EC for three times may be most effective (87% control efficiency) and the least harmful to the tree. Four insecticides tested; 80% dichlorvos EC(dilution of 1250), 47.5% chlorpyrifos EC(dilution of 1000), 2.5% deltamethrin EC (dilution of 2500) and 2% abamectin EC(dilution of 2000) were applied on mango trees during 2 months. Second best was 2% abamectin EC at a dilution of 2000. The other two insecticides were less effective, which might be due to the development of resistance in pests against these insecticides on account of longer repeated applications.[http://en.cnki.com.cn/Article_en/CJFDTOTAL-GXNY200910010.htm]&lt;br /&gt;
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[u]Nettle Caterpillar[/u]&lt;br /&gt;
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The Nettle Caterpillar, aka the Blue-striped Nettle Grub (Parasa lepida aka Latoia lepida; 2 to 3 cm long) has been observed in China, Pakistan, India, Sri Lanka, Bangladesh, Vietnam, Thailand, Malaysia, Japan and especially Indonesia. Parasa lepida may infest various fruit trees, including mango, cherry, persimmon, Indian almond and rose apple, and also coffee, tea, cocoa and palms, with a preference for coconut trees. The caterpillar of this species have spines containing a poison that causes serious irritation and inflammation in humans upon contact. It feeds on the leaves and shoots of the mango tree. Initially, defoliation will be localized, affecting only one or a few trees. Thus the infestation is easily identified. Subsequently, as new generations of this pest emerge, the infested area may rapidly expand. The female moth lays eggs on mango leaves. The cocoons are laid side by side. The cocoons are so hard and stout that they remain on the trunks for 5 to 6 years after spinning. Cocoons from which adults have successfully emerged have clear emergence holes. Newly hatched caterpillars will feed on the underside of the epidermis, stripping it off the leaflets. They often begin where the eggs were laid; at the tip. Then they eat the edges of the leaflet and eat large areas of the lamina. When they have finished developing, the whole leaflet will have been consumed systematically from tip to base, leaving only the midrib. On this midrib, the notched indentations are the only visible remainders left by the caterpillars. [http://www.plantwise.org/KnowledgeBank/Datasheet.aspx?dsid=38935]&lt;br /&gt;
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Parasa lepida is predated on by Agriosphodrus dohrni (The Assassin Bug, native to China, India, Indonesia). This 2 cm long Assasin Bug may be reared (fed every 3 days) on yellow mealworms, in plastic cases, under a temperature of 23°C and RH of 50%.[http://www.researchgate.net/publication/262919166_Taxonomic_and_bionomic_notes_on_Agriosphodrus_dohrni_(Signoret)(Hemiptera_Reduviidae_Harpactorinae)] The Assassin Bug may feed on all kinds of bugs, including aphids, beetles, hoppers&amp;#039;, worms and caterpillars.&lt;br /&gt;
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The great tit (Parus major) is a predator of Parasa lepida cocoons in Japan. The most effective parasites are Apanteles parasae (a parasitoid wasp) and Chaetexorista javana (a parasitoid fly).[http://www.cabdirect.org/abstracts/19820596771.html]&lt;br /&gt;
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Salt spray from the sea and the resulting plant stress (considerable damage) may negatively affect the Parasa lepida moth.[http://www.bioone.org/doi/pdf/10.3956/0031-0603-83.3.193]&lt;br /&gt;
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==Mango Scales==&lt;br /&gt;
[[Image:Coccus_viridis.jpg|thumb|left| Coccus viridis [http://www.insectimages.org/browse/detail.cfm?imgnum=5385208 © J.W. Lotz]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| M. signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
[[Image:Coccus_hesperidum.jpg|thumb|left| Coccus hesperidum [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/09Cocc_hesperidum/4hesperidumHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Tetrastichus.jpg|thumb|right| Tetrastichus [http://bugguide.net/node/view/528762 © Tom Murray]]]&lt;br /&gt;
[[Image:Ceroplastes_floridensis.jpg|thumb|left| Ceroplastes floridensis [http://www.sel.barc.usda.gov/scalekeys/softscales/key/soft_scales/media/html/Species/03Cero_floridensis/4floridensisHabitus.html © Gill]]]&lt;br /&gt;
[[Image:Chilocorus_kuwanae.jpg|thumb|right| C. kuwanae [http://animal.memozee.com/view.php?tid=2&amp;amp;did=10472 © Jinsuk Kim]]]&lt;br /&gt;
[[Image:Aulacaspis_tubercularis.jpg|thumb|left| Aulacaspis tubercularis[http://gaga.biodiv.tw/new23/9409/076.htm]]]&lt;br /&gt;
[[Image:Azya_orbigera.jpg|thumb|right| A. orbigera [http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php © E Arcaya]]]&lt;br /&gt;
[[Image:Drosicha_mangiferae.jpg|thumb|left| D. mangiferae [http://www.jugantor.com/last-page/2014/04/19/89802 ©]]]&lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri.jpg|thumb|right| C. montrouzieri[http://www.ozanimals.com/Insect/Mealybug-Ladybird/Cryptolaemus/montrouzieri.html ©]]]&lt;br /&gt;
[[Image:Rastrococcus_iceryoides.jpg|thumb|left| R. iceryoides [http://www.nbaii.res.in/insectpests/Rastrococcus-iceryoides.php © NBAII]]] &lt;br /&gt;
[[Image:Cryptolaemus_montrouzieri_larva.jpg|thumb|right| C. montrouzieri larva [http://www.insectimages.org/ © J.W. Lotz, FDACS Bugwood.org]]]&lt;br /&gt;
[[Image:Paracoccus_marginatus.jpg|thumb|left| Papaya mealybug [http://www.ablturismo.com/avispa-enviada-por-correo-salva-cultivos-en-la-india/ ©]]]&lt;br /&gt;
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The main mango scales are:&lt;br /&gt;
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* Coccids ; Coccus viridus (soft green scales) and Coccus hesperidum (soft brown scales)&lt;br /&gt;
* wax scales ; Ceroplastes spp.&lt;br /&gt;
* armoured scales ; Aulacaspis tubercularis, Chloropulvinaria polygonata (the Mango Green Shield Scale) and Aspidiotus destructor.&lt;br /&gt;
* Mealybugs (pseudococcidae) ; Drosicha mangiferae, Rastrococcus iceryoides (&amp;quot;Mango mealybugs&amp;quot;) and Paracoccus marginatus (&amp;quot;Papaya mealybug&amp;quot;, which is also a mango pest)&lt;br /&gt;
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Mango scales suck the sap from the leaves, and as a result, the leaves start drying. This may cause branches to die, blemished fruits and premature dropping. Scales are small insects (1 to 7 mm), generally immobile; as if shells are glued to the plant. Only the newly hatched crawlers (emerging from under a big scale) move to their feeding site. Soft scales produce honeydew, which may cause [http://www.waiwiki.org/index.php?title=Mango#Sooty_Mould Sooty mould]. Armoured scales don&amp;#039;t excrete honeydew. The papaya mealybug has been observed in Mexico, Florida, Antigua, Cuba, Dominican Republic, Guadeloupe, Haiti, St Kitts, Martinique, Puerto Rico, St Martin, St Barthélémy, Virgin Islands.[http://www.google.nl/url?sa=t&amp;amp;rct=j&amp;amp;q=&amp;amp;esrc=s&amp;amp;source=web&amp;amp;cd=6&amp;amp;cad=rja&amp;amp;uact=8&amp;amp;ved=0CEIQFjAF&amp;amp;url=http%3A%2F%2Fwww.cabi.org%2FISC%2FFullTextPDF%2F2013%2F20133231104.pdf&amp;amp;ei=VeZ8U_eFNaqX1AXh_YHYDA&amp;amp;usg=AFQjCNGWK-GGi3IRhAfSNBg6nah3XhoKWw&amp;amp;bvm=bv.67229260,d.d2k]&lt;br /&gt;
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[u]Natural enemies[/u]; Natural enemies of scales include parasitic wasps, ladybird beetles and lacewings, which are killed by broad-spectrum pesticides. Because of their honeydew secretion, scales may be protected / farmed by specific ants (eg arboreal ants (Azteca instabilis) farming soft green scales).[http://www.ncbi.nlm.nih.gov/pubmed/18559179] Some fungal pathogens effect this symbiotic interaction.[http://www.ncbi.nlm.nih.gov/pubmed/23905728] Parasitoid insects use the honeydew secreted by coccids (and aphids etc) as an infochemical to locate their hosts.[http://www.ncbi.nlm.nih.gov/pubmed/15112724] R. iceryoides is parasitized by Dinocarsis sp., Microterys flavus, Metastenus concinnus and Tetrastichus sp.[http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
Of the parasitoids Acerophagus papayae and Anagyrus loecki, A. papayae is the most effective in decimating the papaya mealybug. (Meyerdirk)&lt;br /&gt;
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[u]Lacewings[/u]; Most lacewings (also) feed on plant material, such as pollen. Particularly green lacewings such as Mallada boninensis, Mallada astur and Mallada signata are mainly predatory and very effective. They are relatively small (length up to 1.5 cm, including wings). Adults are active during the night, and may be attracted by flashlight. Eggs are attached to leaves and hatch within 4 days. The larval period lasts 12 days, and the pupal period lasts about 9 days. Adults live just one month. One may commercially rear millions of green lacewings for biological control of mango pests.&lt;br /&gt;
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[u]Cryptolaemus montrouzieri[/u] (aka Mealybug Ladybird) is a (4 mm long) predatory ladybird that eats mealybugs, soft scales (Coccidae) and armoured scales (Diaspididae). Originally from Australia, introduced in Florida and commercially available as a mealybug control agent. It needs warm, humid weather. Survival temperature range is 15-35°C. Life cycle on average is 30 days under optimum conditions: 23 °C and 60-70% humidity. Optimal temperature for adults is 28°C. They are active between 16°C and 33°C, and 70 to 80% RH. Longevity of females is 70 days max. It produces 4 generations per year. Development is accelerated at higher temperatures. At 25°C it may lay a total of 200 to 700 eggs (av. 400), at the rate of averagely 9 eggs per day. They preferrably lay their eggs in the egg mass of mealybugs. The eggs hatch in 5 to 6 days. There are 4 larval stages, and the entire larval period lasts about 12 to 17 days. At pupation stage they are about 13 mm long. Adults emerge after 7 to 10 days. These beetles prefer high concentrations of mealybugs to predate on, and will fly away when concentrations of mealybugs are lower.[http://www.arabscientist.org/english/page/11/] During its larval development, one C. montrouzieri may eat 2400 eggs of C. polygonata.[http://www.cabdirect.org/abstracts/19981112241.html] Unfortunately, the filaments produced by its larvae look very similar to mealybugs. Initially release 1 or 2 new active beetles per square meter in the morning, before it gets hot. Subsequently regularly release 2 to 3 beetles per tree. The beetles will be killed by pesticides, but tolerate copper-fungicides.&lt;br /&gt;
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[u]Chilocorus kuwanae[/u]; This ladybird beetle is a primary predator of wax scales (Ceroplastes sp.). This ladybird beetle is attracted by (spraying) methyl jasmonate, mainly due to the terpenoid compound alpha-pinene [http://www.ncbi.nlm.nih.gov/pubmed/19825299]&lt;br /&gt;
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[u]Microweisea coccidivora[/u]; a tiny (1mm long) predatory ladybird beetle from Florida and South Carolina (USA) that feeds particularly on armoured scales.&lt;br /&gt;
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[u]Azya orbigera[/u]; The larvae of this ladybird beetle predate on Coccus viridis. These larvae are relatively immune to ant attacks (ants that farm coccids), due to their sticky waxy filaments. Also, the presence of ants reduces A. orbigera larvae getting parasitized.[http://www.ncbi.nlm.nih.gov/pubmed/18348805] A. orbigera has been observed in Colombia, Guyana, Venezuela, Bélize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, México, USA, Trinidad and the West Indies.[http://www.coccinellidae.cl/paginasWebPeru/Paginas/Azya_orbigera_Peru.php]&lt;br /&gt;
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[u]Sumnius cardoni[/u] (a predatory ladybird) predates on nymphs of D. mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19810586735.html] &lt;br /&gt;
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[u]Coccodiplosis sp[/u] (a parasitic gall midge)[http://www.cabdirect.org/abstracts/19931169342.html;jsessionid=C65B1314198EA578C235959537C88FD9], Cybocephalus sp. (a predatory beetle) and Scymnus coccivora (a predatory ladybird) predate on R. iceryoides (mealybug). &lt;br /&gt;
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[u]Cryptochetum sp.[/u] (parasitic flies) parasitize the 1st- and 2nd-instar nymphs of the Drosicha mangiferae (mealybug).[http://www.cabdirect.org/abstracts/19931169342.html]&lt;br /&gt;
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[u]Coccophagus scutellaris[/u] parasitizes Coccus hesperidum by laying eggs in its gut.[http://www.ncbi.nlm.nih.gov/pubmed/747455] When parasited and parasiteless hosts are available, the female of Coccophagus deposits more eggs on the latter.[http://www.ncbi.nlm.nih.gov/pubmed/7283545]&lt;br /&gt;
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[u]Thomsonisca pakistanensis[/u] is a parasitic wasp that most commonly parasitizes Aspidiotus destructor. A. destructor is also parasitized by Aneristus ceroplastae (tiny parasytic wasp), Comperiella bifasciata (parasytic wasp also effective against yellow scales; Aonidiella citrina[http://www.publish.csiro.au/paper/ZO9710053.htm]), Chartocerus sp. and Chrysonotomyia sp. (both also parasytic wasps) [http://www.cabdirect.org/abstracts/19810586735.html]&lt;br /&gt;
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[u]Aphytis sp[/u]; This tiny (2 to 3 mm long) parasytic wasp parasitizes Aulacaspis tubercularis (up to 46% parasitism in a test).[http://www.actahort.org/books/509/509_96.htm]&lt;br /&gt;
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[u]Lecanicillium lecanii[/u]; This fungus kills various coccids, including Coccus hesperidum.[http://www.ncbi.nlm.nih.gov/pubmed/20863711] Also Ceroplastes sp. may be killed by Lecanicilliurn lecanii. Adding body materials of life coccids to a multi-generation culture (medium), significantly keeps the vigor and higher virulence of this fungus.[http://www.ncbi.nlm.nih.gov/pubmed/20387464]&lt;br /&gt;
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[u]Control[/u]: Collect the affected leaves and burn them. Spraying chlorogenic acid (a phenol naturally present in coffee beans) stimulates locomotory activity of the green scale Coccus viridis, thus minimizing their feeding. [http://www.ncbi.nlm.nih.gov/pubmed/20857759]&lt;br /&gt;
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If you have no other option: Use Metasystox 25% EC at the rate of 0.3 L in 450 L water or Fotidal 50 EC at the rate of 0.5 L in 450 L water / acre. Or spray mineral oil at low concentrations (not damaging the trees) after fruit picking, but not during flowering. Don&amp;#039;t spray during droughts or excessive heat.&lt;br /&gt;
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==Weevils==&lt;br /&gt;
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[u]The Mango Stone Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_mangiferae.jpg|thumb|left| Mango Stone Weevil [http://en.wikipedia.org/wiki/Sternochetus_mangiferae#mediaviewer/File:Mango_seed_weevil_2.jpg © P.Jeganathan]]]&lt;br /&gt;
[[Image:Oecophylla_smaragdina.jpg|thumb|right| Stone Weevil captured by O. smaragdina [http://www.cabi.org/isc/datasheet/16434 © Renkang Peng]]]&lt;br /&gt;
[[Image:Mantis_religiosa.jpg|thumb|right| Praying mantis [http://www.statesymbolsusa.org/Connecticut/insect_praying_mantis.html © Andy Williams / CritterZone]]]&lt;br /&gt;
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The Stone Weevil (Sternochetus/Curculio/Rhynchaenus/Cryptorhynchus mangiferae) aka Mango Seed / Nut Weevil is a short weevil; 7 mm to 1 cm long and 4 mm wide. It possesses a tough exoskeleton. Weevils are a type of beetle. The scales of the Stone Weevil may be of various colours; shades of black, grey, red or yellow. The Stone Weevil has been observed in many mango-producing countries all over the world (including Ghana, Nigeria, Kenya, Mozambique, Tanzania, Uganda, Zambia, India, Bangladesh, Indonesia, Thailand, Malaysia, Australia, USA)[http://www.cabi.org/isc/datasheet/16434]. Adult weevils can hardly fly, and this pest spreads by fruit transports. At dusk and during the night, the adults feed on leaves and young shoots. The female lays her eggs on and just under the surface of the peel of young (about 3 cm long) mangoes. Each female can lay up to 15 eggs per day and may lay several eggs on one fruit. The Stone Weevil prefers the sweetest (high-sugar) varieties of mango. After laying an egg, the weevil will make an incision in the fruit to let the sap from the fruit cover the egg, and to make it stick to the fruit. The amber-coloured sap will harden out, and remain as a protective resin mark over the egg. Newly emerged grubs are white and slender, and have no legs. They bore their way through the pulp towards the seed, within 2 days. Larvae and pupae develop inside the fruit. Pupation occurs in the seed. No external symptoms of attack are readily visible on infested fruits. Once matured (which takes up to 2 months), they eat their way out. About 25% of adult the weevils over-winter in the seed. They can live 2 years. One generation is produced each year.&lt;br /&gt;
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Natural enemies; The Stone Weevil is effectively predated on by Weaver ants such as Oecophylla smaragdina and Oecophylla longinoda.[http://iiste.org/Journals/index.php/JBAH/article/view/12284] Weaver ants, however, may also farm (for the produced honeydew) and protect aphids and various mango scales against their natural enemies. &lt;br /&gt;
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Weevils are also predated on by [http://www.waiwiki.org/index.php?title=Praying_mantis Praying mantids], which may eat anything they can catch, including flies, beetles and moths. Adults readily mate in captivity and have one generation per season. Rearing mantids requires rearing of other insects, such as aphids and drosophilae (for nymphs), tephritids (for young mantids) and moths (for adult mantids) in large quantities. Females lay their eggs in a sticky cluster of several cm long. Nymphs (very tiny praying mantids) emerge from eggs. The developing nymphs need to be separated and fed aphids/drosophilae, or they will become cannibalistic.&lt;br /&gt;
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Control; Collect and destroy all fallen fruits, seeds, leaves, twigs etc. If you have no other option, spraying Fenthion (0.01%) on the trees at the time of oviposition is found to be effective. Fenthion, however, is extremely toxic to beneficial insects [http://www.actahort.org/books/509/509_96.htm], so that applying Fenthion may lead to secondary infestation of Mealybugs. A single spray of tiametoksam (Actara™ SC 240g/ℓ a.i.) at the roots during flower set may be effective for seasonal control.[http://etd.uovs.ac.za/ETD-db/theses/available/etd-09182009-083002/unrestricted/LouwCE.pdf] In Alphonso and Bagan Pali mangoes, a single spray (at dusk) of Monocrotophos 36EC 1ml per 1 litre of water was found to be very effective (97.5% to 100% mortality) [http://issuu.com/kisanadmin/docs/mango1/16] In a laboratory test, Beauveria bassiana was somewhat effective (30% mortality in 14 days), but in an orchard setting there was no effect at all.[http://www.cabdirect.org/abstracts/19971101160.html]&lt;br /&gt;
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[u]The Mango Pulp Weevil[/u]&lt;br /&gt;
[[Image:Sternochetus_frigidus.jpg|thumb|left| Mango Pulp Weevil [http://www.thaibugs.com/?page_id=284 © Thaibugs]]]&lt;br /&gt;
[[Image:Mantis_religiosa_4.jpg|thumb|right| Praying mantis [http://chaos-its-not-just-a-theory.com/tag/biodiversity/ © Wordpress]]]&lt;br /&gt;
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The Mango Pulp Weevil (Sternochetus frigidus or Cryptorrhynchus gravis) has been observed in Bangladesh, India, Indonesia, Malaysia, Myanmar,&lt;br /&gt;
Pakistan, Papua New Guinea, Philippines, Singapore and Thailand. This female weevil lays eggs (25-60 eggs / week) on mango fruits (minimum 6 cm diameter). Newly hatched larvae tunnel into the fruit pulp. The larvae form a chamber (constructed of frass) next to the seed, from which they eat their way through the pulp. Pupation occurs within these chambers. Matured weevils leave the ripe fruit through an exit hole in the peel. Prior to this exit, nothing shows that the fruit is infected. They are fully matured after 6 weeks; able to mate, repeatedly. One complete life cycle varies from 34-35 days. More than half of adult weevils hibernate in seeds. Mango Pulp Weevils are very poor flyers (maximum 90 cm horizontally).[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Thrips==&lt;br /&gt;
[[Image:Frankliniella_occidentalis.jpg|thumb|left| F. occidentalis [http://conabio.inaturalist.org/taxa/243761-Frankliniella © Th0th]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| O. insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Mallada_signata.jpg|thumb|right| Mallada signata [https://www.flickr.com/photos/stevpas68/4124830676/ © Steve Passiow]]]&lt;br /&gt;
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Thrips, or thunderflies, thunderbugs, storm flies, thunderblights, storm bugs, corn flies or corn lice may feed on many plants. Frankliniella bispinosa, Frankliniella kelliae (Florida, Caribbeans) and Frankliniella occidentalis (California, Israel) also feed on mango. They feed on young leaves, shoots and flowers, causing discoloration and deformation.&lt;br /&gt;
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Thrips are tiny (1 mm long on average; varying from 0.5 mm to 1.4 cm). They are not good flyers, but may readily be carried by the wind. Given the right conditions, they may rapidly multiply and form large swarms. To inspect trees for thrips infestation, just hold a bucket and shake a branch, and some thrips will fall off the infested branch into the bucket.&lt;br /&gt;
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Control: Natural enemies of thrips are Green lacewings (eg Mallada boninensis, Mallada signata), Orius insidiosus, Anystis agilis and Hypoaspis aculifer. Biological insecticides such as Beauveria bassiana and Verticillium lecanii may be effective.&lt;br /&gt;
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[u]Orius insidiosus[/u] is a 3 mm long pirate bug (aka insidious flower bug). They feed on thrips, mites, small caterpillars and various insect (including aphids&amp;#039;) eggs and larvae, and also pollen. They prefer eating thrips over aphids.[http://www.ncbi.nlm.nih.gov/pubmed/18845007] Orius may kill several thrips per day, beyond their need for nutrients. Adults fly well, and may readily disperse, looking for prey. O. insidiosus may be mass reared on [http://www.waiwiki.org/index.php?title=Armyworm armyworms]. They may occasionally sting people, which may hurt, but is completely harmless. Eggs are laid and embedded in the plant tissue of the fruit, stem, leave stalks and big veins at the underside of leaves. From eggs to adults, Orius goes through 5 larval stages. This takes about 16 to 18 days at 25°C. They are predatory at each stage (except as pupae). Adults live 3 to 4 weeks. As Orius albidipennis lays more eggs and has a shorter lifecycle, it may be a better biocontrol agent than Orius insidiosus.[http://eurekamag.com/research/030/621/030621437.php] Release at 2 to 3 Orius per square meter.&lt;br /&gt;
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==Whiteflies==&lt;br /&gt;
[[Image:Trialeurodes_vaporariorum.jpg|thumb|left| Trialeurodes vaporariorum [http://en.wikipedia.org/wiki/File:Weisse-Fliege.jpg © Gaucho]]]&lt;br /&gt;
[[Image:Orius insidiosus.jpg|thumb|right| Orius insidiosus [http://en.wikipedia.org/wiki/Orius_insidiosus#mediaviewer/File:Orius_insidiosus_from_USDA_2_(cropped).jpg © J. Dykinga USDA]]]&lt;br /&gt;
[[Image:Paraleyrodes_bondar.jpg|thumb|left| Paraleyrodes bondar [http://manateeornprod.wordpress.com/2012/02/22/new-whitefly-in-florida/ © UF/IFAS]]]&lt;br /&gt;
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Whiteflies (incl. Aleurodicus rugioperculatus, Aleurodicus dispersus, Aleurothrixus floccosus, Aleyrodes proletella, Bemisia tabaci, Paraleyrodes bondar, Siphoninus phillyreae, Trialeurodes vaporariorum) attack various crops, including mango, orange, tomato and watermelon. &lt;br /&gt;
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Whiteflies suck the saps from leaves, causing direct damage. They are also vectors of various diseases, including viruses. Additionally they may produce a lot of honeydew, which may lead to sooty mould. The density of the whitefly is positively correlated with maximum temperature and negatively correlated with relative humidity.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Natural enemies[/u]; Various animals predate on whiteflies, including green lacewings, Orius insidiosus and ladybird beetles. The minute parasitic wasps Encarsia guadeloupae and Encarsia haitiensi very effectively cause reduction in the population of Aleurodicus despersus.[http://14.139.155.167/test5/index.php/kjas/article/viewFile/1644/2462]&lt;br /&gt;
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[u]Control[/u]&lt;br /&gt;
Dissolve 1 kg of sugar per 1 L of water. Poor in plastic transparent bottle. Drill holes in the upper part (above water level); small enough for whiteflies to enter, but not for bees. Put the lid on. Hang 2 to 3 bottles in each tree at flower set. Renew weekly.&lt;br /&gt;
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=Integrated Pest Management=&lt;br /&gt;
Integrated Pest Management (IPM) integrates proper orchard managment (such as sufficient spacing of trees, pruning off overlapping branches, the use of bait traps and removing affected fruits and plant parts) and the use of a variety of anti-bugs (natural enemies of mango bugs), so that they are complementary and sufficiently reduce all pest populations, minimizing the use of pesticides.&lt;br /&gt;
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Below is an example of the complementary use of anti-bugs, for both predation and parasitism.&lt;br /&gt;
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Preliminary requirements: A sealed, ventilated room. Constant supply of mangoes, or grapes, kiwi, lychee, pineapple, peach, apricot, papaya, apple or pear, to feed and rear Drosophilae (2-4mm long &amp;#039;fruit flies&amp;#039;), Tephritids (4-7 mm long &amp;#039;true&amp;#039; fruit flies) and Eudocima sp. (fruit piercing moths; up to 10 cm wingspan). One section needs daylight exposure, for growing Fabacae / Menispermacae for the Eudocima sp. to lay their eggs, and for their larvae to feed on the leaves. This sealed room should rather be too large than too small to prevent a lack of food for the reared predators. Any surplus of eggs and/or larvae may be sold as fish feed.&lt;br /&gt;
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* Rearing [http://www.waiwiki.org/index.php?title=Praying_mantis Praying Mantids] to predate on moths, caterpillars, weevils and young Longicorn beetles by adults, and on fruit flies, hoppers and midgets by young mantids. These mantids may be reared on fruit flies  (Drosophila and Tephretids) and specific moths. Young mantids should be separated after hatching, in boxes. Nymphs should be fed Drosophilae. Young mantids should be fed Tephretids. Adult mantids are fed Eudocima moths or Armyworm moths.&lt;br /&gt;
* Rearing [http://www.waiwiki.org/index.php?title=Mallada_sp. green lacewings] (Mallada signata, Mallada boninensis) to predate on aphids, scales, hoppers and thrips in the field. These lacewings may be reared on Eudocima sp. eggs collected from the Fabacae / Menispermacae in the sealed room, or on Armyworm eggs. &lt;br /&gt;
* Rearing the parasitic wasps Fopius arisanus and/or Diachasmimorpha longicaudata to parasitize Tephritids (fruit flies). Tephritid eggs should be collected from fruits in the sealed room. To prevent the fruit fly eggs from drying out, the eggs should be kept on a moist substrate. To prevent molding, a constant air current should be provided during the time required for parasitoid development.&lt;br /&gt;
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The second phase may be more labour intensive. To rear Tetrastichus sp. and Euplectrus sp. or Trichogramma sp. for the purpose of parasitizing scales, moths, midges and longicorn beetles, one most collect eggs from all species in the field. Instead, one may also rear subject pests.&lt;br /&gt;
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As an alternative for green lacewings, one may use the combination of Cryptolaemus montrouzieri (mass reared on Plancoccus citri on potatoes or squash [http://www.arabscientist.org/english/page/11/], for predating on scales) and Orius insidiosus (mass reared on [http://www.waiwiki.org/index.php?title=Armyworm Armyworms] (which are best reared on Bermudagrass), for predating on aphids, thrips and small caterpillars). This allowes for a more targeted approach (if there is only 1 pest, eg scales). This may alsobe a better combination (together with Praying mantids) because adult Praying mantids prefer to predate on lacewings (15 mm long) over the much smaller C. montrouzieri (4 mm) and O. orius (3 mm).&lt;br /&gt;
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=Diseases=&lt;br /&gt;
Bugs cause damage to the mango tree, which makes the tree more susceptible to disease. Many bugs are also disease vectors; they transport the viruses, fungi etc. to the mango tree. Successfully combatting mango bugs, is therefore key to prevention and control of mango diseases. Using chemicals to fight mango diseases and/or bugs, however, may actually result in killing natural enemies of the bugs that spread disease.&lt;br /&gt;
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==Anthracnose==&lt;br /&gt;
[[Image:Anthracnose.jpg|thumb|left| Anthracnose [http://www.indiancropdiseases.com/mango.aspx]]]&lt;br /&gt;
[[Image:Anthracnose-2.jpg|thumb|right| Anthracnose [http://hawaiiplantdisease.net/cpg/displayimage.php?pid=397 © Nelson]]]&lt;br /&gt;
Anthracnose is caused by the fungi Colletotrichum acutatum [http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2003.93.5.579] and Glomerella cingulata [http://www.idosi.org/wjas/wjas4(6)/8.pdf][http://www.jofamericanscience.org/journals/am-sci/am0608/46_3350am0608_361_367.pdf]. It particularly affects young shoots, flowers and fruits. The disease is stimulated by high humidity, frequent rains or mists, and a temperature range of 24 to 32°C. The results of this disease are black spots on panicles, leaf spot, blossom blight, withered tip, twig blight and fruit rot. Particulary young and tender shoots and foliage are affected, causing die back of young branches. Wounded twigs may also be infected and may sometimes die off. Fruits develop black spots and rotting and young fruits may shrivel and prematurely drop. If the entire inflorescence is destroyed, no fruits will set. &lt;br /&gt;
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[u]Where?[/u] It widely occurs in the orchard and in storage, Anthracnose has been reported in Argentina, Florida, Hawaii, India, Pakistan, Philippines, Sri Lanka, South Africa and Trinidad.&lt;br /&gt;
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[u]Control[/u]: Burn all fallen leaves. Prune affected twigs and burn them. Apply Bordeaux paste on cut ends. To prevent blossom infection, spray twice at 15 days interval during flowering with Carbendazirn (Bavistin 0.1%). To prevent foliar infection, spray copper fungicides (0.3%) twice a week. Apply a teaspoon of dish detergent per spray load to make the copper sulfate stick. Prevent the copper sulfate from dripping on the ground to prevent contamination of the soil and the roots of the tree.&lt;br /&gt;
Picked mangoes should be submerged for 15 minutes in hot water (52°C) with Carbendazim (0.1%). One may also dip the mangoes in 500 ppm Benomyl and 900 ppm Thiobendazole. C. acutatum is also effectively inhibited by the synthetic strigolactone GR24.[http://www.ncbi.nlm.nih.gov/pubmed/21688170]&lt;br /&gt;
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==Bacterial Canker==&lt;br /&gt;
Bacterial black spot (bacterial canker) is a bacterial disease caused by Xanthomonas campestris pv. mangiferaeindicae. It affects many varieties. Initially, irregular small water soaked lesions will appear on the leaves. These will extend into patches of dead tissue. The leaves will turn yellow and die. The lesions will also appear on the the stalk of the leaves, twigs and fruits. The fruits will then turn brown and black, and burst open. The released fluid is filled with bacteria that will contaminate the rest of the tree or other fruits in storage.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Agrimycin-100 (0.01%) or Streptocycline (0.01%), 3 times at 10 days interval. Subsequently spray Copper Oxychloride (0.3%) or Carbendazim (Bavistin 0.1%) once a month.&lt;br /&gt;
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==Die Back==&lt;br /&gt;
Die back (Botryodiplodia (Lasiodiplodia) theobromae aka Physalospora disrupta/quercuum/rhodina/glandicola) may occur any time of the year. It is characterized by advancing discoloration and darkening of the bark. It extends along the veins of the leaves, causing browning and upwards rolling of the margins. Eventually the leaves shrivel and fall. Twigs and branches dry and defoliate, and may drain a yellow-brown gummy fluid. It may look as if the tree has been exposed to a bush fire.&lt;br /&gt;
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[u]Control[/u]: Prune diseased twigs 10 cm below the affected area. Paste the cut ends with Copper Oxychloride (0.3%) and also spray Copper Oxychloride (0.3%) on affected trees.&lt;br /&gt;
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==Diplodia Stem-end Rot==&lt;br /&gt;
This fungus (Lasiodiplodia theobromae) may enter the mango where the skin or stem has been injured mechanically. It will form a black circle around the pedicel.&lt;br /&gt;
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[u]Control[/u]: Prevent mechanical injuries. After picking, submerge mangoes for 15 minutes in hot water (53C) with Carbendazirn (0.1%).&lt;br /&gt;
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==Mango Black Blight==&lt;br /&gt;
Black blight is caused by a fungus (Capnodium mangiferum).&lt;br /&gt;
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==Mango Blight==&lt;br /&gt;
Mango blight is caused by Erwinia bacteria (Enterobacteriaceae). Many spots appear on the leaves which cause a reduction in growth and yield. &lt;br /&gt;
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[u]Control[/u]: Use Dithane M 45 at the rate of 1.7g/L (450 L water per acre).&lt;br /&gt;
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==Mango Malformation==&lt;br /&gt;
Mango malformation (Fusarium mangiferae) has been reported in Australia, Bangladesh, Brazil, Central America, Cuba, Egypt, India, Israel, Malaysia, Mexico, Pakistan, South Africa, Sudan, Switzerland, UAE and the USA. It is a disease caused by fusarium species [http://www.mycologia.org/content/94/4/722.full][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.1999.89.6.456] (F. subglutinans from Egypt, Florida, Israel, Malaysia and South Africa, F. sterilihyphosum from Brazil and South Africa, and Fusarium sp. nov. and F. proliferatum from Malaysia)[http://www.ncbi.nlm.nih.gov/pubmed/18943188][http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-96-0667 Free Full Text]. The disease is stimulated by relatively cool conditions; 10 to 27°C, and merely survive hotter conditions. In this disease the leaves and inflorescence of the mango tree are badly deformed and gradually dry up. There is no fruit setting and hence no production. &lt;br /&gt;
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[u]Control[/u]: Various fungicides, including Captan, Benomyl and Thiram have been reported to be effective to some extend. &lt;br /&gt;
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==Mango Scabs==&lt;br /&gt;
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Mango Scabs (Denticularia mangiferae aka Sphaceloma mangiferae aka Elsinoë mangiferae) survives on young tissues (young leaves, shoots, flower, fruits) of the mango tree only. Adult tissue is not susceptible. Its spores (and their germination) requires rain splash and free water to produce new infections.[http://www.cerambycoidea.com/titles/affa2004.pdf] Moisture conditions stimulate its growth. Though this parasite is not a fungus but in a persistent parasytic relationship with the mango tree, it produces symptoms that look somewhat familiar to those of anthracnose (a fungus) infections. It starts with small light- or dark brown or grey spots on the underside of leaves and fruit. These spots get bigger and darker with time. In the center of this lesion a cracked texture will appear. If the host tissue survives, lesions may develop into scar tissue. When infestation is severe, there is loss of leaves and fruit.&lt;br /&gt;
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[u]Control[/u]; Remove and destroy all fallen leaves, twigs, branches etc. Spray copper hydroxide or copper oxychloride on a very regular basis, particularly in the rainy season. Without chemical control, losses as high as 90% have been observed.[http://www.cerambycoidea.com/titles/affa2004.pdf]&lt;br /&gt;
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==Phoma Blight==&lt;br /&gt;
Phoma blight (Phoma glomerata) is a fungus that affects the fibre in the top layer of old leaves. It produces small, irregular, angular lesions. As the lesions grown, the colour changes from yellow-brown to cinnamon. Many small spots may form overwhelming patches and result defoliation of affected leaves. Phoma glomerate very effectively produces an enzyme that converts the remainders (glucose) of fibre (from the leaves) into it the main structural component (N-acetyl-D-glucosamine)[http://www.ncbi.nlm.nih.gov/pubmed/15553782] in its cell walls, thus enabling its own growth.&lt;br /&gt;
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[u]Control[/u]: Immediately spray Benomyl (0.2%), and 20 days later spray 0.3% Miltox (Copper Oxychloride plus Zineb). Quadris (azoxystrobin) combined with thymol at a non-fungitoxic concentration produces much higher growth inhibition of Phoma glomerata than the fungicide alone.[http://www.ncbi.nlm.nih.gov/pubmed/22408641]&lt;br /&gt;
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==Powdery Mildew==&lt;br /&gt;
Powdery mildew (Oidium mangiferae) affects virtually all mango varieties. Characteristically, a white superficial powdery fungal grows on the inflorescence and tender leaves, stalk of panicles, flowers and young fruits. The affected flowers and fruits will pre-maturely drop, or fruit setting does not occur at all. The disease is initiated and developed by warm (&amp;gt;17°C) dry weather, but during the flowering stimulated by prolonged rains or mists and by cool nights. The disease has been reported in Brazil, India, Jamaica, Pakistan, Sri Lanka, South Africa and the U.S.A. &lt;br /&gt;
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[u]Control[/u]: Alternately spray wettable sulphur (0.2% ; 2 g Sulfex/L), Tridemorph (O.1% ; 1 ml Calixin/L) and Bavistin (0.1%) at 15 days interval. Start spraying when the panicle (flower cluster) starts to emerge. Or spray with Carbendazim (0.1%) or Tridemefon (0.05%) or wettable sulphur (0.3%) alone (3 days in a row).&lt;br /&gt;
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[u]Control-2[/u]: Similar to A. quisqualis AQ10 Biofungicide in commercial use for biocontrol of powdery mildew, Phoma glomerata (see [http://www.waiwiki.org/index.php?title=Mango#Phoma_Blight Phoma Blight] above) may act as mycoparasite of powdery mildew. Phoma glomerata can colonize and suppress development of powdery mildew.[http://www.ncbi.nlm.nih.gov/pubmed/10618259] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91841/ Full free text]&lt;br /&gt;
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==Red Rust==&lt;br /&gt;
Red rust (Cephaleuros virescens aka C. parasiticus) initially causes round and slightly elevated green-grey spots mainly on leaves. They turn into rusty red spots and may form bigger patches. This reduces the photosynthetic capacity of the leaves. In severely infected trees, twigs will remain stunted, the bark and twigs will get thick and the leaves will dry up and drop off. &lt;br /&gt;
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[u]Control[/u]: Spray Copper Oxychloride (0.3%) 3 times.&lt;br /&gt;
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==Sooty Mould==&lt;br /&gt;
Sooty mould (Meliola mangiferae) may be the result of too many insects in your orchard. Scale insects, mealy bug and hoppers secrete honey dew. The honey dew sticks to the leaves, feeding fungal growth. This results in a black sooty mould over the entire surface of leaves and twigs, affecting the photosynthetic capacity of the leaves. Uncontrolled, the tree may completely turn black.&lt;br /&gt;
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[u]Control[/u]: Prune and burn the affected branches. Spray Wettasulf (0.2%) with+ Metacid (0.1 %) and gum acacia (0.3%). Ants feed on the honeydew excreted by soft scales, preventing accumulation of sooty moulds, but they also protect the scales from natural enemies.&lt;/div&gt;</summary>
		<author><name>Aytundra</name></author>
		
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